NCVDLS Annual Report reports... · 2014-2015 North Carolina Department of Agriculture & Consumer...

2014-2015

North Carolina Department of Agriculture & Consumer Services

Veterinary Division

Steven W. Troxler, Commissioner of Agriculture

R. Douglas Meckes, DVM, State Veterinarian

Karen W. Post, DVM, MS, Director of Laboratories

NCVDLS Annual Report Volume 4

10/30/2015

N or th C a ro l in a V e te r i na ry D ia gn os t i c L a bor a to ry Sy s te m

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Table of Contents

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2 Mission Statement/Message from the Director

3-5 Facilities and Laboratory Personnel

6 External Laboratory Advisory Committee

7 Budget and Facility Information

8 Improvements, New Equipment & New Test Procedures

8 Professional Development and Outreach

9 Proficiency Testing

10-12 NCVDLS Accession Information

Rollins Animal Disease

Diagnostic Laboratory

2101 Blue Ridge Road

Raleigh, NC 27607

(919) 733-3986

Western Animal Disease


785 Airport Road

Fletcher, NC 28732

(828) 684-8188

Northwestern Animal Disease


1689 N. Bridge St.

Elkin, NC 28621

(336) 526-2499

Griffin Animal Disease


401 Quarry Rd.

Monroe, NC 28112

(704) 289-6448

NCVDLS Facilities

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On an annual basis, we assemble summaries of the activities within the

North Carolina Veterinary Diagnostic Laboratory System (NCVDLS). The

information contained within is for the period between July 1, 2014 and June 30,

2015. Data from all four laboratories comprising our system are included.

Services have been on the increase, with an escalation of 1.72% overall in

laboratory accessions when compared to the previous fiscal year. We hope you

find this information to be both interesting and useful. Our goal, as always, is to

provide clients with accurate and timely service to meet our Mission, as stated

above. Please do not hesitate to contact me about this annual report, if you have

questions.

Respectfully yours,

Karen W. Post, DVM, MS

Diplomate ACVM

NCVDLS Mission Statement

It is the mission of the North Carolina Veterinary Diagnostic

Laboratory System to provide veterinarians, the animal industry,

and the citizens of North Carolina with accurate and timely

laboratory support services in order to diagnose, conduct

surveillance, and assist in responding to and preventing animal

disease. Both protection of public health and the food supply are

Important components of this mission.

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NCVDLS Facilities and Laboratory Personnel Rollins Animal Disease Diagnostic Laboratory

1031 Mail Service Center

Raleigh, NC 27699-1031

Phone: (919)733-3986

Fax: (919)733-0454

Director of Laboratories Karen W. Post, DVM, MS, D-ACVM

Assistant Director Richard Mock, PhD

Administrative Staff Cheryl Hearn Administrative Officer I, Section Supervisor

Mechelle Johnson Administrative Assistant I

Rebecca Boone Processing Assistant IV

Phyllis Howard Processing Assistant IV

Connie Howen Processing Assistant IV

Paul Miller Processing Assistant III

Cindy Orlosky Processing Assistant V

Brandy Rodgers Processing Assistant IV

Tamara Seago Processing Assistant V

Dell Weaver Medical Laboratory Assistant II

Bacteriology Sandy Murphy, BS, M-ASCP Medical Laboratory Supervisor I, Section Supervisor

Melissa Cox Medical Laboratory Technologist I

Lee Goldbach, MS Medical Laboratory Technologist I

Megan Flowers, BS Medical Laboratory Technician II

Richard Koch Medical Laboratory Technologist I

Elizabeth Ortiz, BS Medical Laboratory Technologist II

Bill O’Shields Medical Laboratory Assistant III

Jeri Smart, BS, Medical Laboratory Technologist I

Sommer Souther, BS Medical Laboratory Technician II

Laura Tweed Medical Laboratory Technologist I

Building Maintenance Stephen Szymczyk Maintenance Mechanic V, Section Supervisor

Joey Stephenson Maintenance Mechanic IV

Information Technology Josh Shepherd Business and Technology Applications Analyst, Section Supervisor

Talley Ouro Business and Technology Applications Analyst

Molecular Diagnostics Beverly Wood, BS, MT-ASCP Laboratory Medical Specialist, Section Supervisor

Neeti Dahal, BS, PhD Medical Laboratory Technologist II

Younghee Lee, BS Medical Laboratory Technologist II

Chad Menard, BS Medical Laboratory Technologist III

Ashlee Zack, BS, Medical Laboratory Technologist II

Pathology Services Coordinator James Trybus, DVM, D-ACVP

Avian Pathologist Tahseen Abdul Aziz, DVM, MS, PhD, D-ACPV

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Anatomic Pathologists Allison Boone, DVM, D-ACVP

Steve Rushton, DVM, D-ACVP

Alison Tucker, MS, VMD, D-ACVP

Diagnosticians Mahogany Caesar, MS, DVM

Jennifer Haugland, DVM

Stacy Robinson, DVM

Necropsy Assistant Allie Wetzel, BS Medical Laboratory Technician II

Histopathology Mary Baker, HT-ASCP Medical Laboratory Technologist III, Section Supervisor

Danice Gregorowicz Medical Laboratory Technologist I

Judy Liverman Medical Laboratory Technician II

Cindy Nipper, HT-ACSP Medical Laboratory Technologist I

Immunohistochemistry David Weil, BS, HT-ASCP Medical Laboratory Technologist I

Quality Assurance and Safety Tina Buffington, BS Medical Laboratory Supervisor III, Quality Manager

Ghazala Jawad, MS Medical Laboratory Supervisor III, Quality Manager

Paul Hutter, BS Safety Officer III, Division Safety Officer

Serology Kristen Crook, BS, PhD Medical Laboratory Technologist III, Section Supervisor

Brandon Brown, BS Medical Laboratory Technologist I

Kim Gooden Medical Laboratory Technologist I

Aisha Phipps, BS Medical Laboratory Technologist I

Virology Chad Cecil, PhD. Medical Laboratory Supervisor III, Section Supervisor

Jonathan Fox, BS Medical Laboratory Technician II

Kim Howle, BA, MS Medical Laboratory Technologist III

Fuhing Lo, BS Medical Laboratory Technician II

Angie Murphy, BS Medical Laboratory Technologist II

Excel Swann, BS Medical Laboratory Technologist I

HuiQing Tang, BS Medical Laboratory Technologist I

Amy Tyson, BS Medical Laboratory Technologist I

NCVDLS Branch Facilities Griffin Animal Disease Diagnostic Laboratory

401 Quarry Road

Monroe, NC 28112

Phone: (704)289-6448

Fax: (704)283-9660

Kim Hagans, DVM Resident Director

Reg Ridenhour, DVM Veterinary Diagnostician

Sherry Casey Processing Assistant IV

Kathy Harwood, AAS Medical Laboratory Technologist I

Carrie Lichtenhan, BS Medical Laboratory Technician II

Kristie Long Medical Laboratory Technician II

Jimmy Walters Medical Laboratory Assistant II

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Northwestern Animal Disease Diagnostic Laboratory

1689 N. Bridge St.

Elkin, NC 28621

Phone: (336)526-2499

Fax: (336)526-2603

Brad Barlow, DVM Resident Director

Jessica Kees, DVM Veterinary Diagnostician

Barbara Bright, BS Medical Laboratory Technologist II

Marcella Galyean , AAS Medical Laboratory Technician II

Nicholas Mosley Veterinary Laboratory Assistant I

Cindy Smith Processing Assistant IV

Western Animal Disease Diagnostic Laboratory

785 Airport Road

Fletcher, NC 28732

Phone: (828)684-8188

Fax: (828)687-3574

Richard Oliver, DVM Resident Director

David Drum, DVM, MS Veterinary Diagnostician

Darlene Ayers Processing Assistant IV

Lia Barth, BS Medical Laboratory Technician II

Wilson Smith Medical Laboratory Assistant II

Mary Swanson, DVM Medical Laboratory Technician II

SERVICE MILESTONES, 2013-2014

RETIREMENTS

Elsie F. Coombs Rollins Laboratory Histology 9/1/2014

Brenda Howell Northwestern Lab Admin 9/1/2014

Phyllis Howard 5 years

Nicholas Mosley 5 years

Connie Howen 5 years

Brandy Rodgers 10 years

Dr. David Drum 10 years

Tamara Seago 10 years

Dr. Kimberly Hagans 15 years

Judy Liverman 15 years

Brandon Brown 15 years

Beverly Wood 25 years

Barbara Bright 25 years

External Laboratory Advisory Committee Members

The NCVDLS established an External Laboratory Advisory Committee (ELAC) in 2005. Our

initial purpose was to better serve our clients by uniting a diverse group of veterinarians and

industry representatives to discuss the laboratory’s offered services, as well as legislation that

directly affected our mission. We are appreciative of their time and advice.

NAME

ADDRESS

Dr. Allen Cannedy

Mixed Practice

Small Ruminant and Camelid Services

110 Merlot Drive

Hillsborough, NC 27278

Dr. Eric Gonder

Poultry – Turkeys

Butterball, LLC

PO Box 10009

Goldsboro, NC 27532

Dr. Jennifer Haugland

NCVDLS

Rollins Diagnostic Laboratory



Dr. Richard Kirkman

Large Animal

20416 US Hwy 64 West

P O Box 350

Siler City, NC 27344

Dr. Shannon Jennings

Poultry – Chickens & Turkeys

House of Raeford

P O Box 699

Rose Hill, NC 28458

Dr. David Marshall

State Veterinarian

NCDA&CS, Veterinary Division



Dr. Richard Mock

NCVDLS




Dr. Karen Post

NCVDLS




Dr. Rick Sharpton

Poultry – Chickens

Perdue, Inc.

1071 Vicksburg Dr.

Winterville, NC 28590

Dr. Betsy Sigmon

Small Animal

Creature Comforts Animal Hospital

3435 Kildaire Farm Road

Cary, NC 27518

Dr. Chad Smith

Corporate Swine

Goldsboro Milling Co.

938 Miller’s Chapel Road

Goldsboro, NC 27532

Dr. Becky Tilley

Poultry – Turkeys

Butterball, LLC

PO Box 10009

Goldsboro, NC 27532

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General Funds, Funded Contracts and Cooperative Agreements 2014-2015 NCVDLS General Funds

2013/2014 Recurring Cooperative or Blanket Purchase Agreements

USDA-APHIS-VS Blanket Purchase Agreement—$24,440

reimbursement for BSE Surveillance Sample Collection and Testing


reimbursement for Scrapie Sample Collection and Testing

USDA-APHIS-WS Blanket Purchase Agreement—$70,000

reimbursement for Swine Influenza sample testing in feral swine


reimbursement for African Swine Fever and Food and Mouth Disease testing


reimbursement for SECD testing by RT-PCR

USDA-AHPIS-VS Swine Surveillance Cooperative Agreement—$62,200

reimbursement for surveillance and testing of Classical Swine Fever and PRV samples

USDA-NIFA Cooperative Agreement—$202,000

to aid the Food and Agriculture Defense Initiative for surveillance and early

detection of animal diseases

Budget Summary 2013/2014 2014/2015

Purchased Contractual Services 5321 $124,583 $174,526

Utility/Energy Services 5322 313,109 $303,378

Repair Services 5323 152,966 $101,969

Maintenance Agreements 5324 63,001 $87,921

Rentals/Leases 5325 6,847 $6,323

Travel & Other Employee Expenses 5327 43,964 $39,217

Communications & Data 5328 100,852 $99,574

Other Services 5329 12,531 $14,602

General Admin Supplies 5331 35,606 $38,141

Facility and Hardware Supplies 5332 31,664 $28,032

Vehicle/Equipment Operating Supplies 5333 1,655 $1,748

Clothing and Recreational Supplies 5335 3,755 $730

Research/Development & Edu Supplies 5337 1,224,343 $1,248,477

Other Materials & Supplies 5339 1,290 $5,811

Equipment 5345 227,235 $107,264

Art, Other Artifacts & Literature 5346 2,000 $1,822

Intangible Assets (Computer software) 5347 35,171 $3,131

Legal, Licenses & Permit Costs 5351 5,991 $6,715

Other Admin Expenses 5358 $0 $4,867

Other Expenses 5359 1,934 $4,029

Transfer of Appropriations 5389 3,754 $80,281

TOTAL 2,392,250 $2,358,558

Personal Services (Salaries, Benefits, etc.) 4,869,938 $5,257,814

TOTAL 7,262,188 $7,616,371

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2014-2015 Physical Improvements/New Equipment Purchases

Rollins Laboratory

Molecular Diagnostics—2 ABI Thermal cyclers, 1 Smart Cycler desktop computer

Serology—Heraeus Labofuge centrifuge, Thermo Upright Ultra Low Freezer

Griffin Laboratory

Xerox copier/fax combination, Toshiba telephone system

Western Laboratory

Labconco Paramount Hood

2014-2015 Test Procedures Added

Rollins Lab Molecular Diagnostics—Equine Herpes Virus Rt-PCR

Rollins Laboratory IHC—PNL2 Cell Marker Test

Rollins Laboratory Serology—Toxoplasma gondii ELISA, Q Fever ELISA

Professional Development and Outreach

FNAME MEETING CITY

BEG

DATE

Dr. Mahogany Caesar 2015 SEVPAC Tifton, GA 5/29/2015

Dr. Chad Cecil 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/15/2014

Dr. Allison Boone ACVP 2014 Annual Meeting Atlanta, GA 11/7/2014

Tina Buffington 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/17/2014

Dr. David Drum Blackmon Annual Equine Encore Conference Athens, GA 11/5/2014

Biomedical/Diagnostic Practice Mngmt Confer-

ence Knoxville, TN 12/3/2014

Dr. Kimberly Hagans 2015 Emerald Coast Veterinary Conference Destin, FL 6/3/2015

Ghazala Jawad 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/15/2014

Dr. Jessica Kees AVMA Certification Ames, IA 9/8/2014

ACVP 2014 Annual Meeting Atlanta, GA 11/9/2014

Dr. Richard Mock 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/16/2014

Dr. Karen Post 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/16/2014

Dr. Reginald Riden-

hour 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/17/2014

Dr. Stacy Robinson 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/16/2014

Dr. Steven Rushton 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/16/2014

Dr. James Trybus 2014 USAHA/AAVLD Annual Meeting Kansas City, MO 10/15/2014

Dr. Alison Tucker ACVP 2014 Annual Meeting Atlanta, GA 11/9/2014

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PROFICIENCY TESTING

All four laboratories of the NCVDLS participate in proficiency testing from outside agencies. In addition to

these external proficiency tests, laboratory sections participate in internal testing.

Blind Sample Tests are administered by section supervisors to random technologists. Samples have “dummy”

External Proficiency Testing Test Laboratory Section Administering

Agency

Internal Bacteriology QA Survey Bacterial culture identification Rollins Lab Bacteriology AAVLD

Salmonella Group D Environmental culture Rollins Lab Bacteriology NPIP

Southern Bug Exchange Inter-

Laboratory Comparison Program

Bacterial culture identification Rollins Lab Bacteriology Consortium of

Southern VDLS

Taylorella equigenitalis Bacterial identification Rollins Lab Bacteriology NVSL

Leptospirosis IHC Panel (Ring Test) Rollins Lab IHC AAVLD/NVSL

Neospora Caninum Ring Test Rollins Lab IHC AAVLD/NVSL

Avian Influenza Matrix Primer/ Probe Sets & H5 Pri-

mer/ Probe Sets, H5 & H7 PCR

Rollins Lab Molecular NAHLN

Avian Paramyxovirus -1 END/NDV for Matrix and Fusion

Primer/ Probe Sets

Rollins Lab Molecular NAHLN

Swine Influenza Virus Rt-PCR Rollins Lab Molecular NAHLN

CSF/FMD Rt-PCR Rollins Lab Molecular NAHLN

MG & MS PCR PCR Rollins Lab Molecular NPIP

African Swine Fever & Rinder-

pest Virus

PCR Rollins Lab Molecular NAHLN

PED and PDCoV PCR Rollins Lab Molecular NAHLN

Brucellosis BAPA, Card Rollins Lab Serology NVSL

Anaplasmosis c-ELISA Rollins Lab Serology NVSL

Johne's Disease ELISA Rollins Lab Serology NVSL

Leptospirosis MAT Rollins Lab Serology NVSL

MG & MS Serology Plate, ELISA & HI Rollins Lab Serology NPIP

Avian Influenza AGID Rollins Lab Virology NVSL

Bovine Leukosis Virus ELISA Rollins Lab Virology NVSL

Pseudorabies ELISA (G1 & GB), Latex Rollins Lab Virology NVSL

Bluetongue ELISA Rollins Lab Virology NVSL

Equine Infectious Anemia AGID Rollins Lab Virology NVSL

West Nile Virus Panel—Equine IgM Capture ELISA Rollins Lab Virology NVSL

Salmonella Group D Environmental culture Griffin Lab Bacteriology NPIP

MG & MS Serology Plate, ELISA & HI Griffin Lab Serology NPIP

Avian Influenza AGID Griffin Lab Virology NVSL

Brucellosis BAPA, Card Western Lab Serology NVSL

Equine Infectious Anemia AGID Western Lab Virology NVSL

Avian Influenza AGID Northwestern Lab Serology NVSL

MG & MS Serology Plate, ELISA Northwestern Lab Serology NPIP

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NCVDLS 2014-2015 *Accession Information

*Accessions may contain more than one test , discipline, and sample.

Avian 1,216,845 92.85%

Bovine 19,066 1.45%

Camelid 1,597 0.12%

Canine 26,955 2.06%

Caprine 6,493 0.50%

Equine 15,190 1.16%

Feline 7,042 0.54%

Fish 107 0.01%

Miscellaneous 2,103 0.16%

Ovine 1,334 0.10%

Porcine 13,819 1.05%

TOTAL 1,310,551 100.00%

Total Test Count by Animal Species

10%13%

74%

3%

Total Accession Count for NCVDLS: 37,483

Increase over 2013-2014 of 1.75%

Griffin Animal Disease Diagnostic Laboratory - 3,589

Northwestern Animal Disease Diagnostic Laboratory - 5,010

Rollins Animal Disease Diagnostic Laboratory - 27,586

Western Animal Disease Diagnostic Laboratory - 1,298

Fiscal Year Comparison of Accessions

FY 13/14 to FY14/15

2013-2014 2014-2015 % Increase

Rollins 27,146 27,586 1.60%

Western 1,269 1,298 2.23%

Northwestern 4,914 5,010 1.92%

Griffin 3,511 3,589 2.17%

Total 36,840 37,483 1.72%

43%

14%

33%

10%

NCVDLS Necropsy Test Count - 7,332

Rollins Laboratory - 3,184 Griffin Laboratory - 1,037

Northwestern Laboratory - 2,398 Western Laboratory - 713

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NCVDLS Total Test Count and Accession Count Comparison

Year Test Count Accession Count

2011-2012 1,261,563 32,583

2012-2013 1,261,919 34,728

2013-2014 1,310,713 36,840

2014-2015 1,327,843 37,483

NCVDLS Test Count Comparison by Laboratory Discipline

Laboratory Discipline 2011-2012 2012-2013 2013-2014 2014-2015

Bacteriology 16,258 17,647 15,843 15,277

Cytology 1,263 1,440 1,601 1,805

Histopathology 38,189 40,940 43,017 44,920

Immunohistochemistry 576 690 594 792

Molecular Diagnostics 9,642 11,689 16,125 15,302

Mycology 263 245 233 252

Necropsy 8,463 7,337 7,844 7,332

Outsource 1,843 1,419 1,299 1,270

Parasitology 2,578 2,801 3,040 3,047

Serology 913,570 886,698 935,650 940,244

Virology 268,849 291,023 285,543 297,602

TOTAL 1,261,494 1,261,929 1,310,789 1,327,843

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Top Twenty-Five Tests 2014-2015 2013-2014 2012-2013

Mycoplasma gallisepticum (ELISA) 348,352 317,774 201,779

Mycoplasma synoviae (ELISA) 345,716 317,526 202,030

Avian Influenza (AGID) 139,647 137,912 130,950

Mycoplasma synoviae (Plate) 85,082 115,098 207,844

Mycoplasma gallisepticum (Plate) 84,925 115,150 207,599

Avian Influenza (ELISA) 82,011 80,209 80,853

Mycoplasma meleagridis (ELISA) 47,522 43,295 41,633

Histopathology H & E 42,213 40,982 38,816

Infectious Bursal Disease (ELISA) 12,848 10,374 10,954

REO (ELISA) 12,520 10,263 11,113

Infectious Bronchitis Virus (ELISA) 12,178 9,410 10,896

Avian Paramyxovirus-1 (APMV-1) (ELISA) 11,951 9,349 10,688

Salmonella pullorum (Plate) 10,800 12,300 9,601

Equine Infectious Anemia (AGID) 10,531 10,868 9,426

Aerobic Culture 7,629 8,014 8,247

Avian Influenza Matrix RRT-PCR 6,643 6,994 5,109

Brucella abortus (BAPA) (Plate) 4,780 4,821 6,440

Gross Exam Poultry 4,032 4,611 4,854

Salmonella pullorum (Tube) 3,736 3,439 3,679

Chicken Anemia Virus 1:10 (CAV) (ELISA) 3,391 3,165 4,137

NPIP Salmonella Culture 3,007 2,655 3,484

Pseudorabies Virus gB (ELISA) 2,411 5,113 4,289

Antimicrobial Susceptibility 2,118 2,189 2,588

Avian Encephalomyelitis Virus (ELISA) 1,906 1,873 1,265

Johne’s ELISA 1,871 1,605 1,267

Accession Counts Comparison

Lab 2011-2012 2012-2013 2013-2014 2014-2015

Rollins Lab 23,885 25,239 27,146 27,586

Griffin Lab 3,156 3,629 3,511 3,589

Northwestern Lab 4,618 4,755 4,914 5,010

Western Lab 924 1,105 1,269 1,298

32,583 34,728 36,840 37,483

Test Counts Comparison

Lab 2011-2012 2012-2013 2013-2014 2014-2015

Rollins Lab 533,173 516,266 511,654 517,174

Griffin Lab 171,836 184,920 210,484 222,132

Northwestern Lab 554,724 558,693 586,088 586,046

Western Lab 1,761 2,050 2,563 2,491

1,261,494 1,261,929 1,310,789 1,327,843

NCVDLS Annual Report reports... · 2014-2015 North Carolina Department of Agriculture & Consumer...

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