Nature Structural & Molecular Biology: doi:10.1038/nsmb · Supplementary Table 1: Mass...
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Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Transcript of Nature Structural & Molecular Biology: doi:10.1038/nsmb · Supplementary Table 1: Mass...
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Supplementary Table 1: Mass spectrometric identification of Lin28-asscociated proteins in mouse ES cells
Gel section
Protein name Accession # Size aa Peptides identified
1 Ddx21 (DEAD (Asp-Glu-Ala-Asp) box 21) NP_062426 851 36 1 Mov10 (Moloney leukemia virus 10) NP_032645 1004 35 1 Zcchc11 (zinc finger, CCHC domain containing 11) NP_780681 1644 3 1 Dhx36 (DEAH (Asp-Glu-Ala-His) box 36) NP_082412 1001 3 2 Pabpc1 (poly(A) binding protein, cytoplasmic 1) NP_032800 636 45 2 Pabpc4 (poly(A) binding protein, cytoplasmic 4) NP_570951 660 23 2 Igf2bp1 (insulin-like growth factor 2 mRNA binding
protein 1) NP_034081 577 9
3 Ssb (Sjogren syndrome antigen B NP_001103615 415 33 3 CSDA (cold shock domain protein A) NP_035863 292 5 4 Ybx1 (Y box protein 1) NP_035862 322 35 4 Ebna1bp2 (EBNA1 binding protein 2) NP_081208 306 3 5 Lin28 NP_665832 209 158* 5 Lin28B NP_001026942 271 6
*Includes 40 peptide sequences obtained from gel sections 1-4
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Replicate 1 Ct
Replicate 2 Ct
Replicate 1 Ct
Replicate 2 Ct
Replicate 1 Ct
Replicate 2 Ct
Replicate 1 Ct
Replicate 2 Ct
Replicate 1 Ct
Replicate 2 Ct
Replicate 1 Ct
Replicate 2 Ct
MammU6-4395470 14.66 14.68 14.31 14.70 14.68 14.71 MammU6-4395470 15.00 14.84 13.75 14.26 14.07 14.03
snoRNA135-4380912 19.98 20.14 19.74 20.10 20.06 19.93 snoRNA135-4380912 20.58 20.38 19.97 20.42 20.44 20.40
snoRNA202-4380914 18.78 18.84 18.35 18.73 18.83 18.86 snoRNA202-4380914 19.08 18.95 18.48 18.89 18.91 18.85
U87-4386735 21.39 21.52 21.24 21.82 21.66 21.94 U87-4386735 21.40 21.40 20.92 20.92 21.50 21.27
Y1-4386739 23.86 23.78 23.81 24.14 23.44 24.22 Y1-4386739 23.98 23.77 22.59 23.39 22.87
ath-miR159a-4373390 35.00 35.00 35.00 35.00 35.00 35.00 ath-miR159a-4373390 35.00 35.00 35.00 35.00 35.00 35.00
mmu-let-7a-4373169 35.00 35.00 35.00 35.00 35.00 35.00 mmu-let-7a-4373169 35.00 35.00 35.00 35.00 35.00 35.00
mmu-let-7b-4373168 28.50 28.97 26.98 27.47 27.88 27.88 mmu-let-7b-4373168 29.22 28.32 27.83 28.43 29.35 29.05
mmu-let-7c-4373167 27.22 26.96 26.05 26.73 26.83 26.93 mmu-let-7c-4373167 27.13 26.82 26.29 26.79 27.20 27.34
mmu-let-7d-4395394 30.65 32.00 25.58 25.95 28.51 28.91 mmu-let-7d-4395394 30.91 31.52 27.09 27.78 28.74 29.47
mmu-let-7e-4395517 28.31 29.04 22.31 22.43 27.11 27.86 mmu-let-7e-4395517 29.09 28.39 23.78 24.34 27.40 26.84
mmu-let-7f-4373164 35.00 35.00 35.00 35.00 35.00 35.00 mmu-let-7f-4373164 35.00 35.00 35.00 35.00 35.00 35.00
mmu-let-7g-4395393 31.00 30.81 24.54 25.27 28.85 29.30 mmu-let-7g-4395393 31.42 29.89 25.98 26.37 29.30 28.98
mmu-let-7i-4395332 33.49 31.81 26.01 26.49 28.38 28.37 mmu-let-7i-4395332 31.95 32.90 27.13 27.77 29.03 29.40
Lin28 siRNA #1 Zcchc11 siRNA #2
Supplementary Table 2. Raw RT-PCR data from P19 siRNA transfections using TaqMan Rodent MicroRNA A Array v2.0
EXPERIMENT 2Control siRNA #2 Lin28 siRNA #2 Zcchc11 siRNA #4Detector Detector
EXPERIMENT 1Control siRNA #1
mmu-miR-100-4373160 29.20 29.88 27.85 28.47 30.92 30.95 mmu-miR-100-4373160 29.97 29.30 30.20 30.10 29.69 29.16
mmu-miR-101a-4395364 23.85 23.84 23.50 23.68 24.22 23.95 mmu-miR-101a-4395364 24.23 24.13 23.95 24.12 24.23 24.03
mmu-miR-103-4373158 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-103-4373158 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-105-4395595 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-105-4395595 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-106a-4395589 16.95 16.94 16.51 16.86 16.94 16.94 mmu-miR-106a-4395589 16.97 16.89 16.33 16.84 17.03 16.86
mmu-miR-106b-4373155 21.68 21.56 21.18 21.59 21.83 21.95 mmu-miR-106b-4373155 21.84 21.57 21.18 21.65 21.73 21.53
mmu-miR-107-4373154 29.36 29.31 28.28 28.36 29.51 30.00 mmu-miR-107-4373154 29.52 29.35 28.08 28.41 28.54 28.75
mmu-miR-10a-4373153 30.15 29.02 28.98 29.98 27.95 28.95 mmu-miR-10a-4373153 29.04 30.23 29.81 31.59 29.10 29.36
mmu-miR-10b-4395329 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-10b-4395329 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-122-4395356 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-122-4395356 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-124-4373295 28.84 28.95 29.85 30.33 30.31 29.49 mmu-miR-124-4373295 29.28 29.26 30.39 29.15 28.54 29.35
mmu-miR-125a-3p-4395310 28.78 28.99 28.53 28.96 29.30 29.09 mmu-miR-125a-3p-4395310 29.87 29.97 29.88 29.62 29.89 29.96
mmu-miR-125a-5p-4395309 24.11 24.10 23.75 23.99 24.52 24.72 mmu-miR-125a-5p-4395309 24.48 24.14 23.97 24.20 24.66 24.56
mmu-miR-125b-3p-4395489 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-125b-3p-4395489 35.00 35.00 35.00 35.00 35.00 35.00
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-125b-5p-4373148 28.24 28.61 27.93 28.11 28.97 28.72 mmu-miR-125b-5p-4373148 28.53 28.33 27.97 28.41 28.41 28.23
mmu-miR-126-3p-4395339 23.68 23.55 23.37 23.85 24.16 24.08 mmu-miR-126-3p-4395339 23.76 23.41 22.97 23.31 23.56 23.41
mmu-miR-126-5p-4373269 26.83 26.79 26.29 26.76 27.37 27.30 mmu-miR-126-5p-4373269 27.11 26.95 26.60 26.97 27.18 26.96
mmu-miR-127-4373147 23.95 23.96 23.71 23.80 24.76 24.30 mmu-miR-127-4373147 24.11 23.85 23.43 23.81 23.95 23.95
mmu-miR-128a-4395327 28.75 28.96 27.81 28.35 28.75 28.66 mmu-miR-128a-4395327 28.71 28.47 27.95 28.57 28.54 28.52
mmu-miR-129-3p-4373297 26.74 26.97 26.97 26.97 26.89 26.95 mmu-miR-129-3p-4373297 26.67 26.43 25.95 26.32 26.67 26.65
mmu-miR-129-5p-4373171 30.13 30.18 29.23 32.72 29.06 29.71 mmu-miR-129-5p-4373171 29.66 30.39 29.76 30.43 29.52 30.03
mmu-miR-130a-4373145 21.96 21.97 21.45 21.96 22.33 22.31 mmu-miR-130a-4373145 22.35 21.97 21.81 22.26 22.38 22.18
mmu-miR-130b-4373144 21.97 21.95 21.47 21.95 22.40 22.48 mmu-miR-130b-4373144 22.25 21.97 21.71 22.14 22.08 22.17
mmu-miR-132-4373143 25.61 26.00 25.41 25.64 24.58 25.08 mmu-miR-132-4373143 26.06 25.92 25.50 26.00 25.83 25.88
mmu-miR-133a-4395357 27.33 27.16 27.03 27.54 27.72 27.87 mmu-miR-133a-4395357 28.00 27.77 27.20 27.50 27.39 27.32
mmu-miR-133b-4395358 30.33 30.80 30.38 31.38 31.74 32.72 mmu-miR-133b-4395358 30.63 30.45 28.77 29.05 32.53 29.46
mmu-miR-134-4373299 24.54 24.64 24.56 24.78 25.07 25.12 mmu-miR-134-4373299 24.86 24.31 23.97 24.28 24.24 24.29
mmu-miR-135a-4373140 27.91 27.97 27.75 27.96 27.85 28.24 mmu-miR-135a-4373140 28.04 27.91 27.95 28.05 28.30 27.97
mmu-miR-135b-4395372 22.93 22.93 22.63 22.49 22.98 22.92 mmu-miR-135b-4395372 23.18 22.96 22.55 23.04 22.94 23.25
mmu-miR-136-4395641 26.14 26.27 26.16 26.17 27.28 26.85 mmu-miR-136-4395641 26.68 26.52 25.96 26.15 26.35 26.16
mmu-miR-137-4373301 35.00 35.00 35.00 35.00 34.03 35.00 mmu-miR-137-4373301 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-138-4395395 25.49 25.46 25.10 25.10 25.36 25.49 mmu-miR-138-4395395 26.01 25.73 25.03 25.48 25.44 25.31
mmu-miR-139-3p-4395676 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-139-3p-4395676 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-139-5p-4395400 25.26 25.34 24.84 25.06 25.65 25.78 mmu-miR-139-5p-4395400 25.97 25.90 25.38 25.60 25.77 25.74
mmu-miR-140-4373374 23.96 23.98 23.60 23.93 24.08 24.07 mmu-miR-140-4373374 24.43 24.18 23.60 23.91 23.95 23.81
mmu-miR-141-4373137 28.43 28.46 27.66 27.97 28.39 28.17 mmu-miR-141-4373137 28.95 28.97 28.64 28.84 28.41 28.35
mmu-miR-142-3p-4373136 30.76 30.99 30.98 31.03 30.81 30.86 mmu-miR-142-3p-4373136 30.97 31.37 30.74 30.55 30.86 30.78
mmu-miR-142-5p-4395359 35.00 35.00 32.74 33.97 31.95 33.00 mmu-miR-142-5p-4395359 31.85 32.72 30.16 30.52 31.80 32.72
mmu-miR-143-4395360 31.90 31.49 29.04 30.71 31.98 30.84 mmu-miR-143-4395360 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-1-4395333 30.53 30.36 31.03 31.16 33.96 31.78 mmu-miR-1-4395333 30.97 30.24 29.34 30.12 30.16 29.97
mmu-miR-145-4395389 27.43 27.35 26.98 27.20 27.74 27.75 mmu-miR-145-4395389 27.98 27.90 27.17 27.50 28.09 27.80
mmu-miR-146a-4373132 29.98 29.73 29.39 29.41 29.77 29.70 mmu-miR-146a-4373132 29.98 30.59 30.20 30.28 30.73 30.84
mmu-miR-146b-4373178 25.44 25.04 24.96 25.28 25.67 25.64 mmu-miR-146b-4373178 25.27 25.35 24.46 25.08 25.36 25.17
mmu-miR-147-4395373 31.00 30.88 29.45 31.43 35.00 35.00 mmu-miR-147-4395373 30.90 30.18 33.01 29.08 30.67 30.67
mmu-miR-148a-4373130 25.36 25.64 24.96 25.27 25.75 25.47 mmu-miR-148a-4373130 25.82 25.15 25.51 25.23 25.19
mmu-miR-148b-4373129 28.06 28.45 27.97 27.92 28.77 28.42 mmu-miR-148b-4373129 28.62 28.22 27.64 27.96 27.98 27.90
mmu-miR-150-4373127 28.63 28.88 27.97 28.07 28.53 28.82 mmu-miR-150-4373127 29.97 29.81 28.90 29.04 29.73
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-151-3p-4373304 25.96 25.89 25.72 25.63 25.98 25.78 mmu-miR-151-3p-4373304 26.06 25.84 25.66 25.97 25.99 25.75
mmu-miR-152-4395170 25.65 25.67 24.90 24.96 25.52 25.49 mmu-miR-152-4395170 25.86 25.65 24.94 25.25 25.05 25.43
mmu-miR-153-4373305 30.90 31.78 31.18 32.62 34.61 35.00 mmu-miR-153-4373305 34.05 35.00 33.30 35.00 32.21 34.38
mmu-miR-154-4373270 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-154-4373270 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-155-4395701 24.91 24.89 24.52 24.95 24.51 24.50 mmu-miR-155-4395701 25.55 25.52 24.94 25.58 25.52 25.32
mmu-miR-15a-4373123 24.88 24.92 24.36 24.88 24.70 24.96 mmu-miR-15a-4373123 24.90 24.61 24.30 24.78 24.95 24.97
mmu-miR-15b-4373122 23.85 23.80 23.37 23.58 24.02 23.95 mmu-miR-15b-4373122 24.32 23.99 23.66 24.15 24.19 24.03
mmu-miR-16-4373121 19.08 18.99 18.54 18.94 19.48 19.20 mmu-miR-16-4373121 19.53 19.27 18.93 19.49 19.37 19.37
mmu-miR-17-4395419 16.95 16.88 16.56 16.86 16.89 16.82 mmu-miR-17-4395419 17.62 17.44 16.94 17.25 17.46 17.33
mmu-miR-181a-4373117 26.96 26.92 27.00 25.91 27.81 27.83 mmu-miR-181a-4373117 26.98 26.61 26.35 26.28 25.74 26.64
mmu-miR-181c-4373115 29.94 29.66 29.78 30.25 29.89 30.39 mmu-miR-181c-4373115 29.54 29.72 29.94 30.09 29.02 29.89
mmu-miR-182-4395729 21.97 21.96 21.70 22.29 22.13 22.29 mmu-miR-182-4395729 22.44 22.14 21.91 22.10 22.43 22.07
mmu-miR-183-4395380 25.36 25.07 24.96 25.19 25.34 25.53 mmu-miR-183-4395380 25.86 25.83 25.06 25.38 25.97 25.47
mmu-miR-184-4373113 29.95 30.64 29.54 29.97 30.84 30.66 mmu-miR-184-4373113 30.95 30.77 30.75 31.47 30.92 30.81
mmu-miR-185-4395382 27.00 27.12 26.68 27.09 27.05 27.37 mmu-miR-185-4395382 27.88 27.82 27.93 27.98 27.62 27.75
mmu-miR-186-4395396 23.86 23.95 23.71 23.96 24.49 24.46 mmu-miR-186-4395396 24.66 24.44 24.21 24.60 24.63 24.49
mmu-miR-187-4373307 27.86 27.74 27.91 27.99 27.27 27.28 mmu-miR-187-4373307 28.45 28.00 28.06 28.58 28.04 27.72
mmu-miR-188-3p-4395217 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-188-3p-4395217 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-188-5p-4395431 27.61 27.83 27.53 27.95 28.86 28.45 mmu-miR-188-5p-4395431 28.40 28.52 28.06 28.32 28.32 28.39
mmu-miR-18a-4395533 24.97 24.97 24.40 24.71 24.57 24.63 mmu-miR-18a-4395533 24.43 24.24 23.96 24.19 24.37 24.13
mmu-miR-18b-4395596 27.71 27.24 26.98 27.18 27.42 27.76 mmu-miR-18b-4395596 27.98 27.76 27.91 27.99 27.88 27.78
mmu-miR-190-4373110 30.97 31.39 30.56 30.67 31.60 31.24 mmu-miR-190-4373110 31.16 31.76 30.93 30.86 31.18 31.98
mmu-miR-191-4395410 19.63 19.78 19.22 19.56 19.97 19.84 mmu-miR-191-4395410 20.74 20.28 19.94 20.11 20.20 20.03
mmu-miR-192-4373108 26.19 26.42 25.96 26.35 26.24 26.30 mmu-miR-192-4373108 26.90 26.76 26.43 26.96 26.79 26.82
mmu-miR-193-4395361 31.40 31.96 31.08 31.63 32.97 32.72 mmu-miR-193-4395361 32.75 31.80 30.92 30.40 31.12 31.78
mmu-miR-193b-4395597 25.81 25.89 25.62 25.97 25.76 25.71 mmu-miR-193b-4395597 26.53 26.21 25.80 26.15 26.20 26.14
mmu-miR-194-4373106 27.58 27.98 27.74 27.93 27.75 27.62 mmu-miR-194-4373106 27.93 27.78 27.69 27.88 28.21 27.97
mmu-miR-195-4373105 23.86 23.76 23.44 23.95 23.97 24.07 mmu-miR-195-4373105 24.33 24.04 23.84 23.93 24.24 24.26
mmu-miR-196b-4395326 34.58 34.00 34.72 33.63 33.40 34.98 mmu-miR-196b-4395326 30.92 30.59 30.08 29.71 32.57 29.89
mmu-miR-197-4373102 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-197-4373102 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-199a-3p-4395415 28.37 28.39 28.13 28.57 29.14 29.97 mmu-miR-199a-3p-4395415 29.58 29.36 28.85 28.80 29.64 29.03
mmu-miR-199a-5p-4373272 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-199a-5p-4373272 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-19a-4373099 21.96 21.96 21.43 21.69 21.75 21.66 mmu-miR-19a-4373099 22.42 22.31 21.48 21.87 21.96 21.97
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-19b-4373098 16.99 16.81 16.29 16.71 16.49 16.59 mmu-miR-19b-4373098 17.49 17.34 16.48 16.83 16.97 16.83
mmu-miR-200a-4378069 24.97 24.97 24.55 24.81 25.11 25.03 mmu-miR-200a-4378069 25.65 25.65 25.32 25.68 25.44 25.60
mmu-miR-200b-4395362 23.87 23.96 23.46 23.71 24.11 23.92 mmu-miR-200b-4395362 24.73 24.68 23.97 24.22 24.55 24.55
mmu-miR-200c-4395411 24.37 24.27 23.47 23.98 24.13 23.95 mmu-miR-200c-4395411 24.92 24.90 24.37 24.67 24.97 24.97
mmu-miR-201-4395708 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-201-4395708 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-202-3p-4373311 27.98 27.98 27.05 27.75 27.22 27.04 mmu-miR-202-3p-4373311 28.52 28.74 27.57 28.05 28.96 28.99
mmu-miR-202-5p-4395709 30.22 30.05 29.84 29.42 30.22 29.21 mmu-miR-202-5p-4395709 31.65 30.99 31.31 30.56 32.26 32.56
mmu-miR-203-4373095 26.37 26.48 25.96 26.28 26.97 26.54 mmu-miR-203-4373095 26.90 26.74 26.93 26.79 26.96 26.82
mmu-miR-204-4373094 28.46 28.91 28.55 28.17 28.57 29.00 mmu-miR-204-4373094 28.41 28.11 28.98 28.77 28.38 28.72
mmu-miR-205-4373093 27.44 27.29 26.96 27.22 27.66 27.53 mmu-miR-205-4373093 27.94 27.71 27.89 27.99 27.68 27.56
mmu-miR-207-4373314 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-207-4373314 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-208-4373091 32.30 33.66 32.07 33.05 33.77 34.29 mmu-miR-208-4373091 33.30 32.47 33.00 32.12 32.23 33.53
mmu-miR-208b-4395401 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-208b-4395401 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-20a-4373286 18.98 18.82 18.38 18.74 18.40 18.72 mmu-miR-20a-4373286 19.55 19.49 18.96 19.30 19.58 19.54
mmu-miR-20b-4373263 18.95 18.93 18.58 18.61 18.89 18.97 mmu-miR-20b-4373263 19.77 19.55 18.95 19.30 19.39 19.50
mmu-miR-210-4373089 19.37 19.35 18.98 19.12 19.63 19.37 mmu-miR-210-4373089 20.03 19.84 19.34 19.78 19.91 19.69
mmu-miR-211-4373315 27.96 27.67 27.97 28.15 28.70 28.74 mmu-miR-211-4373315 28.33 28.58 28.27 28.11 27.92 28.14
mmu-miR-21-4373090 26.58 26.98 26.99 26.93 27.75 27.93 mmu-miR-21-4373090 27.47 26.62 25.97 26.74 26.98 26.71
mmu-miR-214-4395417 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-214-4395417 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-215-4373316 35.00 35.00 33.93 35.00 35.00 35.00 mmu-miR-215-4373316 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-216a-4395331 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-216a-4395331 35.00 35.00 35.00 35.00 35.00
mmu-miR-216b-4395437 32.78 35.00 32.80 35.00 35.00 35.00 mmu-miR-216b-4395437 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-217-4395686 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-217-4395686 35.00 35.00 35.00 35.00 34.45 34.02
mmu-miR-218-4373081 24.12 24.61 23.94 24.27 24.65 24.61 mmu-miR-218-4373081 25.01 24.86 24.58 24.87 24.90 24.93
mmu-miR-219-4373080 28.27 28.77 27.73 27.21 29.98 29.15 mmu-miR-219-4373080 29.43 29.92 29.61 29.84 30.41
mmu-miR-220-4395598 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-220-4395598 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-221-4373077 26.11 26.19 25.73 26.08 26.62 27.01 mmu-miR-221-4373077 26.41 26.34 25.95 26.19 26.44 26.05
mmu-miR-222-4395387 24.26 24.25 23.94 24.18 24.44 24.55 mmu-miR-222-4395387 24.98 24.56 24.44 24.71 24.86 24.87
mmu-miR-223-4395406 30.63 30.64 29.72 30.40 31.22 30.16 mmu-miR-223-4395406 35.00 33.60 35.00 35.00 33.04 32.33
mmu-miR-224-4395683 26.88 26.84 26.83 26.97 27.38 27.81 mmu-miR-224-4395683 27.29 27.09 26.98 27.12 27.48 27.21
mmu-miR-23a-4373074 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-23a-4373074 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-23b-4373073 28.60 28.41 27.96 27.99 28.79 28.98 mmu-miR-23b-4373073 28.50 28.28 27.96 28.37 28.68 28.32
mmu-miR-24-4373072 21.57 21.70 21.24 21.51 22.04 21.97 mmu-miR-24-4373072 22.16 21.89 21.24 21.59 21.75 21.51
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-25-4373071 23.45 23.31 22.93 23.18 23.33 23.33 mmu-miR-25-4373071 23.26 23.14 22.96 23.22 23.53 23.31
mmu-miR-26a-4395166 22.73 22.70 22.31 22.67 22.96 22.90 mmu-miR-26a-4395166 23.12 22.89 22.49 22.94 23.09 22.96
mmu-miR-26b-4395167 25.97 25.88 25.26 25.74 26.29 26.30 mmu-miR-26b-4395167 26.01 25.95 25.38 25.99 26.11 26.21
mmu-miR-27a-4373287 26.94 26.98 26.56 26.63 26.65 26.92 mmu-miR-27a-4373287 26.99 26.53 26.30 26.64 26.65 26.52
mmu-miR-27b-4373068 24.85 24.85 24.30 24.66 25.11 24.94 mmu-miR-27b-4373068 24.94 24.80 24.35 24.77 24.95 24.73
mmu-miR-28-4373067 27.46 27.32 26.96 27.30 27.81 27.63 mmu-miR-28-4373067 27.90 27.72 26.95 27.59 27.87 27.63
mmu-miR-290-3p-4395721 23.85 23.97 23.34 23.77 24.38 24.33 mmu-miR-290-3p-4395721 24.66 24.31 23.87 24.15 24.00 23.83
mmu-miR-291a-3p-4395722 20.37 20.38 19.95 20.24 20.79 20.57 mmu-miR-291a-3p-4395722 21.13 20.94 20.52 20.79 20.92 20.96
mmu-miR-291b-5p-4395667 25.34 25.51 22.51 25.26 25.79 25.95 mmu-miR-291b-5p-4395667 25.93 25.78 26.00 26.14 25.94 25.91
mmu-miR-292-3p-4395723 17.59 17.59 16.95 17.14 17.84 17.47 mmu-miR-292-3p-4395723 18.50 18.22 17.41 17.99 18.52 18.25
mmu-miR-293-4386754 18.97 18.81 18.35 18.87 19.37 19.24 mmu-miR-293-4386754 19.28 19.29 18.63 19.14 19.43 19.37
mmu-miR-294-4373326 19.13 19.23 18.60 18.97 19.44 19.25 mmu-miR-294-4373326 19.76 19.56 18.98 19.49 19.82 19.93
mmu-miR-295-4373327 18.41 18.55 17.93 18.32 18.81 18.97 mmu-miR-295-4373327 19.19 18.97 18.36 18.80 19.31 19.40
mmu-miR-296-3p-4395212 25.99 25.95 25.73 26.22 26.02 25.99 mmu-miR-296-3p-4395212 26.00 25.68 24.91 25.31 25.98 25.87
mmu-miR-296-5p-4373066 23.42 23.70 22.80 22.98 23.89 23.81 mmu-miR-296-5p-4373066 22.95 22.68 22.23 22.94 22.80 23.04
mmu-miR-297b-5p-4381049 31.61 31.26 33.47 32.62 30.36 31.30 mmu-miR-297b-5p-4381049 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-297c-4395610 28.46 27.11 28.33 27.49 28.18 27.95 mmu-miR-297c-4395610 27.87 27.99 27.95 29.27 29.74 29.68
mmu-miR-298-4395728 24.38 24.22 23.95 24.07 24.82 24.86 mmu-miR-298-4395728 24.75 24.36 23.53 24.06 23.62 23.53
mmu-miR-29a-4395223 22.77 22.75 22.63 22.93 22.93 22.93 mmu-miR-29a-4395223 23.51 23.30 22.73 23.33 23.51 23.07
mmu-miR-29b-4373288 28.97 28.94 28.80 29.33 29.15 29.13 mmu-miR-29b-4373288 29.90 29.34 28.97 29.01 29.47 29.42
mmu-miR-29c-4395171 27.21 27.21 26.99 27.42 27.15 27.72 mmu-miR-29c-4395171 27.82 27.91 27.27 27.81 27.98 28.03
mmu-miR-301a-4373064 21.97 21.97 21.56 21.89 22.05 22.47 mmu-miR-301a-4373064 22.34 22.19 21.38 21.80 21.91 21.99
mmu-miR-301b-4395730 22.40 22.40 21.90 22.17 22.96 22.72 mmu-miR-301b-4395730 23.08 22.97 22.30 22.66 22.60 22.62
mmu-miR-302a-4378070 18.30 18.20 17.97 17.99 18.47 18.35 mmu-miR-302a-4378070 18.33 18.07 17.96 18.12 18.21 18.12
mmu-miR-302b-4378071 16.98 16.96 16.44 16.96 17.03 17.12 mmu-miR-302b-4378071 17.72 17.62 16.96 17.31 17.48 17.40
mmu-miR-302c-4395688 19.91 19.66 19.33 19.64 19.98 19.95 mmu-miR-302c-4395688 19.95 19.96 19.11 19.89 19.74 19.64
mmu-miR-302d-4373063 16.95 16.95 16.61 16.93 16.92 17.10 mmu-miR-302d-4373063 17.36 17.30 16.95 17.44 17.46 17.54
mmu-miR-30a-4373061 25.45 25.52 24.95 25.33 25.52 25.38 mmu-miR-30a-4373061 25.95 25.74 25.44 25.75 25.80 25.71
mmu-miR-30b-4373290 21.95 21.98 21.38 21.77 21.65 21.84 mmu-miR-30b-4373290 22.13 21.96 21.38 21.78 21.97 21.92
mmu-miR-30c-4373060 22.01 21.95 21.62 22.03 21.72 21.87 mmu-miR-30c-4373060 22.36 22.28 21.34 21.94 22.17 22.03
mmu-miR-30d-4373059 26.63 26.65 26.05 25.51 26.91 26.52 mmu-miR-30d-4373059 26.92 26.97 26.12 27.15 27.00 26.83
mmu-miR-30e-4395334 23.44 23.47 22.92 23.23 23.48 23.49 mmu-miR-30e-4395334 24.05 23.95 23.62 23.93 24.04 23.94
mmu-miR-31-4373331 24.96 24.92 24.47 24.97 24.40 24.74 mmu-miR-31-4373331 25.49 25.29 24.96 25.43 25.54 25.26
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-320-4395388 23.90 23.69 23.41 23.97 23.71 23.99 mmu-miR-320-4395388 24.26 24.17 23.96 24.30 24.24 24.33
mmu-miR-322-4378107 26.99 26.82 26.48 26.77 27.19 26.90 mmu-miR-322-4378107 26.77 26.97 26.21 26.60 26.85 26.85
mmu-miR-323-3p-4395338 26.34 26.39 25.96 26.32 27.15 27.31 mmu-miR-323-3p-4395338 26.82 26.87 26.27 26.49 26.64 26.99
mmu-miR-32-4395220 29.92 30.30 29.76 29.97 30.43 30.73 mmu-miR-32-4395220 30.73 30.15 30.11 30.27 31.05 30.72
mmu-miR-324-3p-4395639 26.95 27.00 25.63 26.12 26.47 26.62 mmu-miR-324-3p-4395639 27.24 27.06 26.24 26.84 26.82 27.02
mmu-miR-324-5p-4373052 26.84 26.77 25.64 25.79 26.96 26.65 mmu-miR-324-5p-4373052 27.59 26.90 25.96 26.62 26.62 26.66
mmu-miR-325-4395640 30.22 30.59 29.86 31.01 30.24 30.87 mmu-miR-325-4395640 29.92 30.77 29.81 30.45 30.93 31.01
mmu-miR-328-4373049 25.86 25.87 25.27 25.63 25.73 25.98 mmu-miR-328-4373049 26.34 26.48 25.76 26.33 25.97 25.98
mmu-miR-329-4373336 30.00 30.20 30.41 30.97 32.48 31.20 mmu-miR-329-4373336 30.44 29.95 30.24 30.35 30.62 30.88
mmu-miR-330-4395341 27.91 28.27 28.36 30.24 28.30 30.70 mmu-miR-330-4395341 29.33 28.01 29.82 29.07 29.09
mmu-miR-331-3p-4373046 25.33 25.46 25.09 25.75 25.66 25.97 mmu-miR-331-3p-4373046 26.20 25.89 25.57 26.06 25.54 25.46
mmu-miR-331-5p-4395344 29.84 30.99 30.14 30.23 31.03 30.05 mmu-miR-331-5p-4395344 30.97 30.78 29.74 30.68 29.72 29.41
mmu-miR-335-3p-4395296 24.75 24.98 24.57 24.86 24.98 24.98 mmu-miR-335-3p-4395296 25.18 24.93 24.45 24.77 24.99 24.82
mmu-miR-335-5p-4373045 24.24 24.17 23.97 24.06 24.74 24.47 mmu-miR-335-5p-4373045 24.79 24.56 23.96 24.57 24.55 24.52
mmu-miR-337-3p-4395662 28.51 28.57 28.67 28.81 29.12 28.96 mmu-miR-337-3p-4395662 29.42 28.66 28.81 29.12 28.66 28.97
mmu-miR-337-5p-4395645 29.56 29.53 29.44 29.51 29.93 29.98 mmu-miR-337-5p-4395645 30.17 29.76 29.16 30.07 29.81 29.97
mmu-miR-338-3p-4395363 31.35 31.94 30.21 31.53 31.24 31.43 mmu-miR-338-3p-4395363 33.06 31.95 29.62 30.89 30.52 32.24
mmu-miR-339-3p-4395663 26.14 25.98 25.69 25.68 26.31 26.01 mmu-miR-339-3p-4395663 26.61 26.39 26.23 26.63 26.20 26.59
mmu-miR-339-5p-4395368 28.89 29.53 28.82 29.02 28.52 29.59 mmu-miR-339-5p-4395368 29.38 30.11 28.06 28.76 27.75 28.87
mmu-miR-340-3p-4395370 28.08 27.96 27.53 27.59 28.23 28.30 mmu-miR-340-3p-4395370 28.99 28.64 28.20 28.35 27.19 28.68
mmu-miR-340-5p-4395369 26.75 26.68 25.32 25.85 26.96 26.99 mmu-miR-340-5p-4395369 26.97 26.96 26.15 26.62 27.02 27.11
mmu-miR-342-3p-4395371 23.13 23.15 22.96 23.03 23.28 23.28 mmu-miR-342-3p-4395371 23.51 24.39 24.14 24.53 24.31
mmu-miR-342-5p-4395657 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-342-5p-4395657 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-344-4373340 25.76 25.89 25.66 25.98 25.46 25.62 mmu-miR-344-4373340 26.20 26.01 25.97 26.28 25.31
mmu-miR-345-3p-4395659 35.00 35.00 31.88 31.54 32.82 34.79 mmu-miR-345-3p-4395659 30.98 30.77 31.97 31.35 31.24 30.90
mmu-miR-345-5p-4395658 28.73 28.99 28.01 28.36 28.80 28.53 mmu-miR-345-5p-4395658 29.51 29.28 28.29 29.40 28.98 28.64
mmu-miR-346-4373342 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-346-4373342 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-34a-4395168 24.43 24.99 24.62 23.95 24.14 24.24 mmu-miR-34a-4395168 24.36 24.58 24.02 24.85 25.01 24.36
mmu-miR-34b-3p-4395748 25.28 25.30 24.97 25.32 25.20 25.46 mmu-miR-34b-3p-4395748 25.81 25.67 25.18 25.78 25.96 25.78
mmu-miR-34c-4373036 28.01 27.84 27.84 27.89 27.92 27.95 mmu-miR-34c-4373036 28.38 27.98 27.88 27.59 28.30 28.20
mmu-miR-350-4395660 28.70 28.85 28.05 28.31 28.96 28.50 mmu-miR-350-4395660 28.48 28.56 28.39 28.97 29.42 29.23
mmu-miR-351-4373345 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-351-4373345 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-361-4373035 26.71 26.92 26.41 26.98 27.19 27.05 mmu-miR-361-4373035 27.22 26.97 26.36 27.04 27.17 27.00
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-362-3p-4395746 30.95 30.93 30.73 30.73 31.46 31.97 mmu-miR-362-3p-4395746 30.50 30.65 29.70 30.55 31.24 30.95
mmu-miR-363-4378090 33.46 34.04 30.70 31.11 31.46 32.10 mmu-miR-363-4378090 35.00 35.00 35.00 33.80 35.00 35.00
mmu-miR-365-4373194 26.88 26.94 26.34 26.57 27.18 27.44 mmu-miR-365-4373194 26.52 26.85 26.08 26.43 26.97 26.91
mmu-miR-367-4373034 18.47 18.63 17.95 18.36 18.79 18.74 mmu-miR-367-4373034 18.65 18.55 17.95 18.58 18.70 18.61
mmu-miR-369-3p-4373032 34.17 33.13 32.33 34.00 34.26 35.00 mmu-miR-369-3p-4373032 32.19 32.07 31.66 32.79 35.00 35.00
mmu-miR-369-5p-4373195 30.79 30.91 30.71 30.95 31.96 31.97 mmu-miR-369-5p-4373195 31.13 30.95 30.60 31.28 30.91 30.86
mmu-miR-370-4395386 24.41 24.41 23.97 24.34 24.87 24.93 mmu-miR-370-4395386 24.90 24.61 24.14 24.64 24.49 24.44
mmu-miR-375-4373027 26.95 26.80 26.39 26.71 27.23 27.61 mmu-miR-375-4373027 27.03 26.69 26.70 26.95 27.38 27.65
mmu-miR-376a-4373347 24.96 24.96 24.65 24.96 25.78 25.78 mmu-miR-376a-4373347 25.30 25.03 24.66 24.95 25.11 25.09
mmu-miR-376b-4395582 25.14 25.33 24.97 25.23 25.53 25.73 mmu-miR-376b-4395582 25.97 25.67 25.22 25.53 25.88 25.99
mmu-miR-376c-4395580 25.87 25.89 25.66 25.96 26.48 26.37 mmu-miR-376c-4395580 26.46 26.29 25.76 26.12 26.47 26.37
mmu-miR-377-4373025 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-377-4373025 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-379-4373349 23.87 23.87 23.56 23.94 24.47 24.40 mmu-miR-379-4373349 23.86 23.67 23.28 23.65 23.95 23.87
mmu-miR-380-3p-4373350 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-380-3p-4373350 31.97 32.57 35.00 32.85 35.00 35.00
mmu-miR-380-5p-4395731 28.97 29.11 28.17 28.73 29.91 29.98 mmu-miR-380-5p-4395731 29.62 29.66 28.74 29.18 29.54 29.98
mmu-miR-381-4373020 30.99 28.98 27.40 28.06 28.38 29.76 mmu-miR-381-4373020 31.27 30.73 30.99 31.07 31.32 31.01
mmu-miR-382-4373019 25.76 25.98 24.87 25.99 26.16 27.11 mmu-miR-382-4373019 25.19 24.98 24.67 25.05 24.90 24.55
mmu-miR-383-4381093 32.79 30.40 34.80 34.64 32.80 33.01 mmu-miR-383-4381093 30.35 30.61 30.10 32.99 31.56 31.56
mmu-miR-384-3p-4395733 29.02 29.26 28.39 28.77 29.65 31.00 mmu-miR-384-3p-4395733 29.94 29.54 28.09 28.36 28.65 29.02
mmu-miR-384-5p-4395732 26.37 26.38 25.95 26.44 26.73 26.83 mmu-miR-384-5p-4395732 27.00 26.81 26.09 26.51 26.99 26.62
mmu-miR-409-3p-4395443 21.96 21.97 21.59 21.97 22.77 22.89 mmu-miR-409-3p-4395443 21.93 21.43 20.60 21.42 21.39 21.38
mmu-miR-409-5p-4395442 32.62 30.16 28.95 31.50 29.89 31.94 mmu-miR-409-5p-4395442 30.33 30.82 31.04 30.89 31.37 30.97
mmu-miR-410-4378093 25.37 25.34 24.96 25.30 25.97 26.14 mmu-miR-410-4378093 25.96 25.70 25.16 25.58 25.94 25.72
mmu-miR-411-4381013 23.52 23.31 23.21 23.57 23.98 23.80 mmu-miR-411-4381013 24.52 24.20 23.96 24.19 24.33 24.32
mmu-miR-423-5p-4395451 27.75 28.00 27.22 27.51 27.48 27.82 mmu-miR-423-5p-4395451 27.13 27.33 26.67 27.15 27.73 27.98
mmu-miR-425-4380926 27.70 27.36 26.97 27.76 26.84 27.80 mmu-miR-425-4380926 26.73 26.72 26.53 26.94 26.62 26.97
mmu-miR-429-4373355 24.65 24.75 23.95 24.36 24.02 24.90 mmu-miR-429-4373355 24.94 24.98 23.10 24.74 24.83 24.77
mmu-miR-431-4395173 27.45 27.21 27.79 27.45 28.32 29.44 mmu-miR-431-4395173 27.84 27.61 27.89 28.45 28.02 25.92
mmu-miR-433-4373205 25.60 25.55 24.87 24.99 25.94 25.94 mmu-miR-433-4373205 26.00 25.94 25.35 25.96 25.69 25.84
mmu-miR-434-3p-4395734 25.92 25.98 25.66 25.90 26.41 26.64 mmu-miR-434-3p-4395734 26.76 26.53 25.98 26.46 26.59 26.48
mmu-miR-434-5p-4395711 28.25 28.19 28.28 28.83 28.82 28.99 mmu-miR-434-5p-4395711 28.82 28.87 28.29 28.58 28.97 28.58
mmu-miR-448-4373206 30.91 31.78 32.68 32.97 35.00 35.00 mmu-miR-448-4373206 31.88 32.68 33.09 32.24 35.00 32.96
mmu-miR-449a-4373207 27.93 28.23 28.13 28.24 28.34 28.32 mmu-miR-449a-4373207 28.85 28.71 28.62 28.54 28.96 28.30
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-449b-4395669 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-449b-4395669 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-450a-5p-4395414 28.82 28.92 28.40 28.75 28.95 28.92 mmu-miR-450a-5p-4395414 28.94 28.54 28.27 28.54 28.69 28.75
mmu-miR-450b-5p-4386779 35.00 35.00 33.59 35.00 35.00 35.00 mmu-miR-450b-5p-4386779 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-451-4373360 35.00 35.00 35.00 35.00 32.70 32.96 mmu-miR-451-4373360 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-452-4373281 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-452-4373281 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-453-4395614 35.00 33.62 35.00 35.00 35.00 35.00 mmu-miR-453-4395614 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-455-4395585 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-455-4395585 30.82 30.30 32.77 35.00 33.06 35.00
mmu-miR-464-4373362 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-464-4373362 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-465a-3p-4395574 30.79 31.98 30.02 32.05 32.19 32.33 mmu-miR-465a-3p-4395574 35.00 35.00 35.00 35.00 35.00 34.98
mmu-miR-465a-5p-4373363 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-465a-5p-4373363 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-465b-5p-4395615 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-465b-5p-4395615 35.00 35.00 32.33 33.25 34.23 34.01
mmu-miR-466h-4395646 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-466h-4395646 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-467a-4395717 26.66 26.63 26.43 26.69 27.09 26.96 mmu-miR-467a-4395717 27.01 26.89 26.48 26.90 26.96 27.01
mmu-miR-467b-4381084 28.32 28.35 27.61 28.15 28.01 28.25 mmu-miR-467b-4381084 28.97 28.84 27.53 28.46 28.97 28.96
mmu-miR-467c-4395647 27.98 27.64 27.37 27.41 27.93 27.83 mmu-miR-467c-4395647 27.86 27.69 26.98 27.55 28.04 27.99
mmu-miR-467d-4395648 30.39 30.42 30.91 31.37 30.01 31.00 mmu-miR-467d-4395648 30.66 30.87 30.41 30.52 30.97 30.77
mmu-miR-467e-4395698 29.87 29.97 28.94 29.76 29.98 30.48 mmu-miR-467e-4395698 29.28 29.79 29.03 29.44 29.57 29.98
mmu-miR-468-4373366 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-468-4373366 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-469-4373367 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-469-4373367 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-470-4395718 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-470-4395718 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-484-4381032 21.60 21.56 21.05 21.34 21.18 21.58 mmu-miR-484-4381032 21.93 21.97 21.48 21.74 21.87 21.77
mmu-miR-486-4378096 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-486-4378096 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-487b-4378102 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-487b-4378102 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-488-4381074 30.95 30.20 29.96 30.19 31.51 30.95 mmu-miR-488-4381074 30.86 30.38 30.80 32.12 30.33 31.37
mmu-miR-489-4378114 23.80 23.75 23.59 24.00 24.28 24.43 mmu-miR-489-4378114 24.97 24.89 24.27 24.66 24.99 24.97
mmu-miR-490-4373215 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-490-4373215 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-491-4381053 28.08 27.96 27.73 27.99 28.10 28.99 mmu-miR-491-4381053 28.86 28.46 27.08 27.79 28.38 28.22
mmu-miR-493-4395649 30.39 30.07 30.27 30.31 30.74 29.96 mmu-miR-493-4395649 30.57 30.32 29.37 30.76 29.70 29.08
mmu-miR-494-4395476 24.77 24.81 24.50 24.96 25.39 25.35 mmu-miR-494-4395476 25.74 25.72 24.98 25.35 25.77 25.62
mmu-miR-495-4381078 25.73 25.61 24.95 25.33 25.98 25.78 mmu-miR-495-4381078 25.83 25.61 24.96 25.36 25.60 25.67
mmu-miR-496-4386771 28.79 31.29 28.40 29.82 29.28 29.20 mmu-miR-496-4386771 31.02 30.44 30.27 31.35 31.35 33.54
mmu-miR-497-4381046 27.91 27.81 27.15 27.71 28.36 27.96 mmu-miR-497-4381046 28.79 28.38 27.64 27.98 28.20 28.17
mmu-miR-499-4381047 35.00 33.73 34.71 35.00 34.80 35.00 mmu-miR-499-4381047 35.00 35.00 33.85 32.29 35.00 35.00
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-500-4395736 30.97 31.97 32.24 32.57 31.43 31.63 mmu-miR-500-4395736 32.79 33.00 31.45 31.19 30.70 30.30
mmu-miR-501-3p-4381069 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-501-3p-4381069 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-503-4395586 27.52 27.88 27.98 28.37 27.43 27.38 mmu-miR-503-4395586 30.22 28.91 29.00 29.41 29.62 28.28
mmu-miR-504-4395195 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-504-4395195 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-505-4381071 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-505-4381071 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-509-3p-4395651 32.73 32.84 33.45 35.00 31.25 32.49 mmu-miR-509-3p-4395651 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-509-5p-4395650 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-509-5p-4395650 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-511-4395679 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-511-4395679 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-532-3p-4395466 26.92 26.95 26.82 27.03 27.53 27.51 mmu-miR-532-3p-4395466 27.63 27.49 26.96 27.52 27.61 27.38
mmu-miR-532-5p-4380928 25.27 24.97 25.00 25.41 25.80 25.91 mmu-miR-532-5p-4380928 25.50 25.50 24.97 25.37 25.66 25.69
mmu-miR-539-4378103 29.51 30.40 30.78 32.53 29.85 31.24 mmu-miR-539-4378103 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-540-3p-4378119 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-540-3p-4378119 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-540-5p-4395691 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-540-5p-4395691 32.90 31.69 30.82 31.65 35.00 35.00
mmu-miR-542-3p-4378101 29.90 29.97 29.30 29.74 29.97 29.44 mmu-miR-542-3p-4378101 30.91 30.68 29.98 30.58 29.96 29.97
mmu-miR-542-5p-4395693 27.66 27.52 27.16 27.49 27.60 27.99 mmu-miR-542-5p-4395693 27.77 27.59 27.16 27.49 27.80 27.72
mmu-miR-543-4395487 26.39 26.39 25.85 26.23 26.72 26.98 mmu-miR-543-4395487 26.67 26.62 25.97 26.22 26.77 26.54
mmu-miR-544-4395680 31.67 32.15 31.96 31.82 35.00 33.97 mmu-miR-544-4395680 31.67 31.63 30.82 32.35 32.92 32.98
mmu-miR-546-4381044 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-546-4381044 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-547-4395694 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-547-4395694 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-551b-4380945 34.84 33.14 32.40 32.83 35.00 35.00 mmu-miR-551b-4380945 34.63 33.96 32.84 34.27 35.00 35.00
mmu-miR-574-3p-4395460 27.99 27.94 27.63 27.87 27.90 27.95 mmu-miR-574-3p-4395460 28.44 27.94 27.79 28.46 28.68 28.96
mmu-miR-582-3p-4395697 33.39 33.41 31.18 31.61 33.06 31.74 mmu-miR-582-3p-4395697 35.00 35.00 33.99 34.30 35.00 35.00
mmu-miR-582-5p-4395696 32.15 32.19 33.80 34.45 32.69 32.02 mmu-miR-582-5p-4395696 32.72 33.06 34.88 34.66 35.00 35.00
mmu-miR-590-5p-4395176 34.07 33.86 32.49 33.41 35.00 32.10 mmu-miR-590-5p-4395176 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-598-4395606 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-598-4395606 35.00 35.00 35.00 35.00 34.52 35.00
mmu-miR-615-3p-4386777 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-615-3p-4386777 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-615-5p-4395464 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-615-5p-4395464 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-652-4395463 26.22 26.33 25.78 25.99 26.14 26.50 mmu-miR-652-4395463 26.90 26.98 25.92 26.64 26.62 26.77
mmu-miR-654-3p-4395350 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-654-3p-4395350 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-654-5p-4395652 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-654-5p-4395652 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-665-4395737 32.49 33.49 32.59 32.40 33.01 32.74 mmu-miR-665-4395737 32.13 33.00 32.03 33.38 32.66 32.78
mmu-miR-666-5p-4386770 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-666-5p-4386770 32.13 32.71 31.19 31.90 33.11 31.79
mmu-miR-667-4386769 25.33 25.22 24.44 24.87 25.59 26.00 mmu-miR-667-4386769 25.42 25.08 24.72 25.00 24.99 24.72
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-668-4386767 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-668-4386767 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-669a-4381091 27.73 27.47 27.77 27.93 27.98 27.81 mmu-miR-669a-4381091 27.97 27.26 27.11 27.73 27.63 27.63
mmu-miR-670-4395561 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-670-4395561 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-671-3p-4395433 29.85 30.07 29.94 29.84 29.64 29.96 mmu-miR-671-3p-4395433 30.97 30.40 30.30 30.48 29.95 30.62
mmu-miR-672-4395438 25.83 25.70 25.44 25.97 25.95 25.54 mmu-miR-672-4395438 26.54 25.94 25.82 26.35 26.42 26.30
mmu-miR-674-4395193 26.92 26.90 26.62 27.00 27.03 27.07 mmu-miR-674-4395193 27.45 26.98 26.62 27.28 27.32 27.86
mmu-miR-675-3p-4386762 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-675-3p-4386762 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-675-5p-4386761 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-675-5p-4386761 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-676-4386776 25.55 25.32 24.99 25.32 25.99 25.83 mmu-miR-676-4386776 26.02 25.98 25.65 25.61 26.42 25.97
mmu-miR-677-4381075 31.22 30.72 30.19 31.20 32.00 31.32 mmu-miR-677-4381075 30.00 29.70 28.50 29.45 31.73 32.62
mmu-miR-679-4381077 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-679-4381077 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-680-4381079 27.32 27.49 26.98 27.32 27.42 27.80 mmu-miR-680-4381079 28.23 27.93 27.15 27.56 28.33 27.96
mmu-miR-682-4381081 25.23 24.83 25.00 25.56 24.92 mmu-miR-682-4381081 27.23 26.99 25.93 26.72 25.95 26.57
mmu-miR-683-4381082 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-683-4381082 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-684-4381083 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-684-4381083 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-685-4386748 23.96 23.93 23.67 23.94 23.54 23.70 mmu-miR-685-4386748 24.63 24.35 24.58 24.60 24.99 24.65
mmu-miR-686-4381085 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-686-4381085 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-687-4386750 22.70 22.83 22.67 22.60 22.95 22.71 mmu-miR-687-4386750 24.01 23.93 23.16 23.23 23.01 22.88
mmu-miR-708-4395452 23.64 23.53 22.95 23.40 23.63 23.88 mmu-miR-708-4395452 23.50 23.31 22.94 23.32 23.59 23.38
mmu-miR-741-4395587 31.77 30.95 30.54 30.85 32.01 31.26 mmu-miR-741-4395587 32.01 32.64 31.49 31.10 31.12 30.93
mmu-miR-742-4395573 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-742-4395573 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-743a-4395599 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-743a-4395599 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-743b-3p-4395601 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-743b-3p-4395601 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-743b-5p-4395600 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-743b-5p-4395600 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-744-4395435 26.06 25.95 25.44 25.82 26.68 26.59 mmu-miR-744-4395435 26.97 26.91 26.64 27.01 26.96 26.95
mmu-miR-770-3p-4395564 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-770-3p-4395564 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-7a-4378130 29.12 28.95 28.84 29.14 28.69 28.68 mmu-miR-7a-4378130 29.29 28.60 28.64 28.78 28.95 28.73
mmu-miR-7b-4395685 28.87 28.51 28.56 28.97 28.43 28.51 mmu-miR-7b-4395685 28.86 28.72 28.09 28.66 28.98 28.78
mmu-miR-802-4395566 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-802-4395566 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-871-4395465 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-871-4395465 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-872-4395375 25.94 25.72 25.38 26.02 26.38 26.17 mmu-miR-872-4395375 26.93 26.41 26.30 26.42 26.72 26.22
mmu-miR-873-4395467 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-873-4395467 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-874-4395379 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-874-4395379 35.00 35.00 35.00 35.00 35.00 35.00
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
mmu-miR-875-3p-4395677 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-875-3p-4395677 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-876-3p-4395594 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-876-3p-4395594 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-876-5p-4395593 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-876-5p-4395593 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-878-5p-4395670 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-878-5p-4395670 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-879-4395602 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-879-4395602 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-881-4395739 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-881-4395739 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-883a-3p-4395591 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-883a-3p-4395591 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-883a-5p-4395741 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-883a-5p-4395741 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-883b-3p-4395695 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-883b-3p-4395695 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-92a-4373013 18.93 18.95 18.27 18.71 18.62 18.63 mmu-miR-92a-4373013 18.87 18.59 18.34 18.85 18.96 18.77
mmu-miR-93-4373302 21.00 20.72 20.60 20.97 21.28 21.11 mmu-miR-93-4373302 22.10 22.07 21.46 21.91 21.97 21.68
mmu-miR-9-4373285 26.98 26.97 26.63 26.82 27.65 27.53 mmu-miR-9-4373285 27.76 27.44 26.96 27.26 27.78 27.50
mmu-miR-96-4373372 29.85 30.39 29.62 29.98 30.56 30.45 mmu-miR-96-4373372 30.10 30.25 29.76 29.24 31.09 30.45
mmu-miR-98-4373009 35.00 35.00 35.00 35.00 35.00 35.00 mmu-miR-98-4373009 35.00 35.00 35.00 35.00 35.00 35.00
mmu-miR-99a-4373008 27.75 30.00 27.22 27.78 28.25 26.41 mmu-miR-99a-4373008 28.12 28.36 28.17 28.11 28.40 28.01
mmu-miR-99b-4373007 22.98 22.75 22.21 22.75 23.09 23.45 mmu-miR-99b-4373007 22.97 22.54 22.22 22.94 23.27 22.94
rno-miR-1-4395765 31.76 32.83 31.98 32.95 31.62 32.59 rno-miR-1-4395765 31.42 32.18 30.96 31.56 32.69 30.81
rno-miR-17-3p-4395779 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-17-3p-4395779 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-190b-4395749 30.72 31.36 30.50 30.83 31.97 32.94 rno-miR-190b-4395749 30.94 31.19 31.00 30.48 32.00 31.17
rno-miR-196c-4395750 29.99 30.26 29.56 29.56 29.97 30.44 rno-miR-196c-4395750 31.14 30.96 30.19 30.59 30.97 30.52
rno-miR-207-4381096 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-207-4381096 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-20b-3p-4395753 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-20b-3p-4395753 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-219-1-3p-4395778 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-219-1-3p-4395778 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-219-2-3p-4395501 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-219-2-3p-4395501 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-224-4373187 26.71 26.71 25.97 26.47 26.99 26.96 rno-miR-224-4373187 27.36 26.97 26.38 26.58 26.99 26.98
rno-miR-327-4381108 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-327-4381108 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-333-4381109 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-333-4381109 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-336-4381111 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-336-4381111 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-339-3p-4395760 26.03 26.78 25.91 26.23 26.90 26.99 rno-miR-339-3p-4395760 27.06 27.09 26.99 27.04 26.23 26.96
rno-miR-343-4381123 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-343-4381123 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-344-3p-4381112 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-344-3p-4381112 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-344-5p-4395761 35.00 35.00 35.00 35.00 34.22 35.00 rno-miR-344-5p-4395761 35.00 35.00 35.00 35.00 34.16 35.00
rno-miR-345-3p-4395762 29.71 29.79 29.43 29.40 29.96 29.96 rno-miR-345-3p-4395762 30.18 29.81 29.46 30.26 30.23 29.95
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
rno-miR-346-4381113 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-346-4381113 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-347-4381114 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-347-4381114 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-349-4381115 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-349-4381115 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-351-4395764 32.00 32.04 31.66 32.17 31.89 32.38 rno-miR-351-4395764 33.99 32.89 31.90 32.76 32.31 32.53
rno-miR-377-4381100 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-377-4381100 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-381-4381102 28.59 27.99 27.50 28.00 28.80 28.99 rno-miR-381-4381102 29.38 28.98 28.86 28.43 28.58 28.49
rno-miR-409-5p-4381098 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-409-5p-4381098 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-421-4381122 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-421-4381122 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-450a-4381124 29.99 29.60 29.75 29.67 29.59 28.87 rno-miR-450a-4381124 29.38 29.52 27.94 27.95 30.00 29.36
rno-miR-466b-4395767 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-466b-4395767 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-466c-4395768 30.00 31.54 30.56 31.77 30.54 31.99 rno-miR-466c-4395768 31.80 32.03 29.95 30.96 32.80 33.11
rno-miR-505-4381097 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-505-4381097 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-532-5p-4395752 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-532-5p-4395752 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-543-4395766 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-543-4395766 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-598-5p-4395754 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-598-5p-4395754 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-673-4395755 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-673-4395755 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-742-4395756 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-742-4395756 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-743b-4395769 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-743b-4395769 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-758-4395180 28.00 27.94 27.75 27.85 28.76 28.82 rno-miR-758-4395180 28.67 28.22 27.98 28.27 28.43 27.99
rno-miR-760-5p-4395758 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-760-5p-4395758 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-871-4395770 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-871-4395770 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-878-4395771 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-878-4395771 35.00 35.00 35.00 35.00 35.00 35.00
rno-miR-881-4395773 35.00 35.00 35.00 35.00 35.00 35.00 rno-miR-881-4395773 35.00 35.00 35.00 35.00 35.00 35.00
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Supplementary Table 3. SiRNAs used in this study.
ON-TARGETplus siRNA duplex
Target Sequence (5’→3’) Dharmacon Catalog Number
Control siRNA #1 *Proprietary D-001810-01-05 Control siRNA #2 *Proprietary D-001810-02-05 Lin28 siRNA #1 GGGUUGUGAUGACAGGCAA Custom Lin28 siRNA #2 CGUGAUGGUUGAUAGCUAA Custom
Lin28B siRNA #1 GCCUUUAGCCUGCGAACUA J-063393-09 Lin28B siRNA #2 GGAAGUAUGGUGUGUGAUA J-063393-10 Lin28B siRNA #3 GCACUCAGAUCAAGUUAUA J-063393-11 Lin28B siRNA #4 GGUAUAAAAUCUAGGGAAG J-063393-12
Zcchc11 siRNA #1 GCAAAUCUCCUUUAACAUU J-065226-05 Zcchc11 siRNA #2 GGGCUAAGCUGUGCUAUAU J-065226-06 Zcchc11 siRNA #3 GAAAAGCAGUGAUGUUAAU J-065226-07 Zcchc11 siRNA #4 CCAAAGUGCCUAUUGUAAA J-065226-08 Zcchc6 siRNA #1 GAAUUAAUAUCACGGGAAU J-050846-09 Zcchc6 siRNA #2 AGAUCAGGCUUCAACGUAA J-050846-10 Zcchc6 siRNA #3 GAAAGUGAGGCGACGGAGA J-050846-11 Zcchc6 siRNA #4 AGAUCAGGCUCGUGAACAU J-050846-12
Pols siRNA #1 GGUGAAACGGAUCGAAACU J-050846-09 Pols siRNA #2 CCCAGGAAGUGAUCGACUA J-050846-10 Pols siRNA #3 AAAUGAUGUUGGACGGAGU J-050846-11 Pols siRNA #4 UGACAUUGACUCCGACACA J-050846-12
Papd4 siRNA #1 AAGCAGUGAUGGCGAUUUA J-065383-09 Papd4 siRNA #2 CUGUUAGAACCUCGAGAAA J-065383-10 Papd4 siRNA #3 GUUCCACCUCUUCGAGAAA J-065383-11 Papd4 siRNA #4 GGAAGAGAATAAGCGAUGA J-065383-12 Papd5 siRNA #1 CGUGAAAGUUCUAGACAAA Custom Papd5 siRNA #2 GGAUCGAGAGUGUGAUUAA Custom
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
Supplementary Table 4. TaqMan assays used in this study.
Gene Name Applied Biosystems Catalog Number
Gapdh Mm99999915_g1 Lin28 Mm00524077_m1
Lin28B Mm01190675_m1 Zcchc11 (TUTase 4/Papd3/Hs3) Mm00615428_m1
Zcchc6 (TUTase 7/Cid1/Papd6/Hs2) Mm00463475_m1 Pols (TUTase 5/Trf4-1) Mm01349513_m1
Papd4 (TUTase 2/GLD2) Mm00505733_g1 Papd5 (TUTase 3/Trf4-2) Mm01244128_m1
snoRNA-142 4380913 let-7a 4373169 let-7g 4395393
miR-21 4373090
Nature Structural & Molecular Biology: doi:10.1038/nsmb.1676
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