Curriculum vitae

Name: EDUARD AYUSO LÓPEZ Date: 26/04/2014

CURRENT RESUME Last Name: Ayuso Lopez First Name: Eduard Date of birth: July 20th 1977 Address: Institut de Recherche Therapeutique-Universite de Nantes City: Nantes State/Province: Pays-de-la-Loire Zip/Postal Code: 44000 Country: France Home address: 10 Rue Bonnamen City: Nantes State/Province: Pays-de-la-Loire Zip/Postal Code: 44000 Country: France Languages: Spanish, English, Catalan, Italian, French. Degree Degree: Veterinary Medicine Year awarded: 2001 University, College, or Institution: School of Veterinary Medicine. Universitat Autònoma de Barcelona. Spain Degree: Master in Biochemistry and Molecular Biology. Year awarded: 2003 University, College, or Institution: Universitat Autònoma de Barcelona. Spain Degree: PhD, with the European Mention, in Biochemistry and Molecular Biology. Year awarded: 2006 University, College, or Institution: Universitat Autònoma de Barcelona. Spain Areas of expertise -Viral vectors (Ad, AAV and HdAd): vector design, vector manufacturing, and vector purification -In vivo gene transfer in small and large animal models

Professional experience Since September 2013.- Senior Researcher at the “Institut National de la Santé et de la Rechercher Médicale” (INSERM), Unit UMR 1089, being part of Atlantic Gene Therapies (http://www.atlantic-gene-therapies.fr/) at Nantes (France). July 2011-August 2013. Group leader. Center of Animal Biology and Gene Therapy. Universitat Autònoma de Barcelona. Barcelona. Spain. July 2006-2011. Research associate in Dr. Fatima Bosch Lab, Center of Animal Biology and Gene Therapy. Universitat Autònoma de Barcelona. Barcelona. Spain. 2007. Visitor in Dr. Katherine High lab (Children’s Hospital of Philadelphia, Philadelphia, USA) to learn new technologies for AAV production. 2001-2006 June. Ph.D Student of Biochemistry and Molecular Biology in Dr. Fatima Bosch Lab, Center of Animal Biology and Gene Therapy. Universitat Autònoma de Barcelona. Barcelona. Spain. 2005. Travel Grant. Stage (8 months) in Dr. Mauro Giacca lab. Molecular Medicine Group. International Center of Genetic Engineering and Biotechnology. Trieste. Italy. 2004. Travel grant. Stage (1 month) in Dr. Stefan Kochanek lab. Division of Gene Therapy. University of Ulm. Ulm. Germany. 2000-2001. Recipient of Student collaborator fellowship in Dr. Fatima Bosch Lab. Department of Biochemistry and Molecular Biology. Universitat Autònoma de Barcelona. Barcelona. Spain. 2000. Recipient of Socrates-Erasmus fellowship in Dr. Antonella Baldi Lab. Institute of Animal Nutrition. School of Veterinary Medicine. Università degli Studi di Milano. Milan. Italy. 1996-2000. Student collaborator in Dr. Fatima Bosch Lab. Department of Biochemistry and Molecular Biology. Universitat Autònoma de Barcelona. Barcelona. Spain.

HONOURS 2012. Extraordinary doctorate price. Awarded by the Universitat Autonoma de Barcelona. 2011. EASD/JDRF/Roche: Young Investigator Award in Innovative Therapy for Type 1 Diabetes. 2009. Young Scientist Award of CLINIGENE (European Network for the Advancement of Clinical Gene Transfer and Therapy). 2007. Young Scientist Award of EUGENE2 (European Network on Functional Genomics of Type 2 Diabetes). 2002-2005. Fellowship for the Ph. D studies “Beca de Formació de Personal Investigador”. Generalitat de Catalunya. Spain. 2005. Travel grant from Generalitat de Catalunya for a stage in Dr. Mauro Giacca lab. Molecular Medicine Group. International Center of Genetic Engineering and Biotechnology. Trieste. Italy. 2004. Travel grant from Generalitat de Catalunya for a stage in Dr. Stephan Kochanek lab. Division of Gene Therapy. University of Ulm. Ulm. Germany. 2003. Grant from “Fundación Botín” to attend the meeting: II Reunion de la Sociedad Española de Terapia Génica. Barcelona. Spain. 2000-2001. Student collaborator fellowship in the Department of Biochemistry and Molecular Biology. Universitat Autònoma de Barcelona. Spain. 2000. Socrates-Erasmus fellowship

COORDINATOR OF I+D PROJECTS PROJECT TITLE: Conversion of acinar- to β-cells in situ by controlled

expression of transcription factors FOUNDED BY: EFSD/JDRF/Roche PARTICIPANTS: Universidad Autónoma de Barcelona DURATION: 2 years START: 2011 END: 2013 MAIN INVESTIGATOR: Eduard Ayuso PARTICIPATION IN I+D PROJECTS PROJECT TITLE: Ajut per a potenciar els grups de recerca de qualitat en el

marc del Pla de Recerca de Catalunya. FOUNDED BY: CIRIT, Generalitat de Catalunya (2001SGR 00195) PARTICIPANTS: Universidad Autónoma de Barcelona DURATION: 3 years START: 2002 END: 2004 MAIN INVESTIGATOR: Fàtima Bosch Tubert PROJECT TITLE: Red de Centros: Determinantes moleculares del

metabolismo y la nutrición. Biocomunicación hormonal. Nuevas estrategias terapeúticas. Enfermedades del Metabolismo y de la Nutrición.

FOUNDED BY: INSTITUTO DE SALUD CARLOS III (C03/08) PARTICIPANTS: Universidad Autónoma de Barcelona DURATION: 3 years START: 2003 END: 2005 MAIN INVESTIGATOR: Fàtima Bosch Tubert PROJECT TITLE: Red de Grupos: Red de enfermedades metabólicas

hereditarias (REDEMETH). Enfermedades metabólicas hereditarias avances en diagnóstico clínico, bioquímico y bases moleculares y etiopatogénesis. Nuevas aproximaciones terapéuticas. Estudios epidemológicos.

FOUNDED BY: INSTITUTO DE SALUD CARLOS III (G03/054) PARTICIPANTS: Universidad Autónoma de Barcelona DURATION: 3 years START: 2003 END: 2005 MAIN INVESTIGATOR (CBATEG): Fàtima Bosch PROJECT TITLE: Red de Grupos: Defectos metabólicos y moleculares en

la diabetes mellitus y sus complicaciones. Terapia génica y celular. Diabetes mellitus y sus complicaciones.

FOUNDED BY: INSTITUTO DE SALUD CARLOS III (G03/212) PARTICIPANTS: Universidad Autónoma de Barcelona

DURATION: 3 years START: 2003 END: 2005 MAIN INVESTIGATOR: Fàtima Bosch Tubert PROJECT TITLE: European Network on Functional Genomics of Type 2

Diabetes (EUGENE2) FOUNDED BY: EU, Network of Excellence. LIFESCIHEALTH.

(NoE 512013) DURATION: 4years. START:November 2004. END:November 2008 MAIN INVESTIGATOR: Fàtima Bosch PROJECT COORDINATOR: Ulf Smith, Göteborg University, Sweden (13 groups PROJECT TITLE: Beta Cell Programming for Treatment of Diabetes

(BETACELLTHERAPY) FOUNDED BY: EU, Integrated Project (Nº 512145) DURATION: 5 years. START: January 2005 END: January 2010 MAIN INVESTIGATOR : Fàtima Bosch PROJECT CORDINATOR: Daniel Pipeleers, Vrije University, Brussel-VUB PROJECT TITLE: European Network for the Advancement of Clinical

Gene Transfer and Therapy (CLINIGENE) FOUNDED BY: EU, Network of Excellence. FP6-2004-

LIFESCIHEALTH-5 (No 018933) DURATION: 5 years START: 2006 END: 2011 MAIN INVESTIGATOR: Fàtima Bosch PROJECT CORDINATOR: Odile Cohen-Haguenauer, Ecole Normale Supérieure de

Cachan, France. PROJECT TITLE: Red de Grupos: Defectos moleculares en la Diabetes

Mellitus: Terapia génica y celular FUNDED BY: INSTITUTO DE SALUD CARLOS III (G03/212) DURATION: 1 year START: 2006 END: 2007 MAIN INVESTIGATOR: Fàtima Bosch PROJECT TITLE: Red de Centros: Diabetes y Enfermedades Metabólicas

(REDIMET) FUNDED BY: INSTITUTO DE SALUD CARLOS III

(RD06/0015/0033) DURATION: 1 year START: 2007 END: 2008 MAIN INVESTIGATOR: Fàtima Bosch PROJECT TITLE: CIBERDEM: Diabetes y Enfermedades Metabólicas FUNDED BY: INSTITUTO DE SALUD CARLOS III

(CB07/08/0037) DURATION: 4 years START: 2008 END: 2011

MAIN INVESTIGATOR: Fàtima Bosch PROJECT TITLE: Estudio del papel de los factores de crecimiento

similares a la insulina en la regeneracion de la celula ß pancreatica

FUNDED BY: Ministerio de Educación y Ciencia, Plan Nacional I+D+I (SAF2008-00962)

DURATION: 3 years START: 2009 END: 2011 MAIN INVESTIGATOR: Fàtima Bosch PROJECT TITLE: Ajut per a grups de recerca consolidats FUNDED BY: Agència de Gestió d´Ajuts Universitaris i de Recerca

(AGAUR), Generalitat de Catalunya (2009 SGR 224) DURATION: 5 years START: 2009 END: 2013 MAIN INVESTIGATOR: Fàtima Bosch PROJECT TITLE: Desarrollo de vectores adeno-associados (AAV) para la

terapia neuroprotectora en enfermedades degenerativas de la retina.

FUNDED BY: Proyectos TRACER. Ministerio de Ciencia e Innovación, en colaboración con ProRetina Therapeutics S.L. (PET2008_0282_02)

DURATION: 2 years START: 2009 END: 2011 MAIN INVESTIGATOR: Enrique de la Rosa and Fàtima Bosch

PARICIPATION IN I+D PROJECTS WITH COMPANIES:

PROJECT TITLE: Desarrollo de vectores adeno-associados (AAV) para la terapia neuroprotectora en enfermedades degenerativas de la retina.

SOURCE OF SUPPORT: ProRetina Therapeutics S.L. FUNDING DATES: 2 years FROM: 2009 TO: 2011 PRINCIPAL INVESTIGATOR: Fàtima Bosch PROJECT TITLE: Gene Therapy Approaches for Mucopolysaccharidosis

IIIA. SOURCE OF SUPPORT: Laboratorios Dr. Esteve S.A. FUNDING DATES: 18 months FROM: July 2009 TO: December 2010

PROJECT TITLE: Gene Therapy Approaches for Mucopolysaccharidosis IIIA: Continuation of preclinical studies

SOURCE OF SUPPORT: Laboratorios Dr. Esteve S.A. FUNDING DATES: 2 years FROM: January 2011 TO: December 2012 PRINCIPAL INVESTIGATOR: Fàtima Bosch PROJECT TITLE: Gene Therapy Approaches for Mucopolysaccharidosis

IIIB, IIIC, IIID. SOURCE OF SUPPORT: Laboratorios Dr. Esteve S.A. FUNDING DATES: 2 years FROM: January 2011 TO: December 2012 PRINCIPAL INVESTIGATOR: Fàtima Bosch

PATENTS:

TITLE: Vectors and sequences for the treatment of diseases. HOLDER: Universidad Autonoma de Barcelona and Laboratorios del Dr.

Esteve S.A. INVENTORS: Fàtima Bosch, Albert Ruzo and Eduard Ayuso. REQUEST NO: EP10382169.0 PRIORITY COUNTRY: Europe PRIORITY DATE: 10 June 2010 EXTENDED TO COUNTRIES: European Patent COMPANIES EXPLOITING IT: Laboratorios del Dr. Esteve S.A. TITLE: Telomerase reverse transcriptase for protection against ageing. HOLDER: Universidad Autonoma de Barcelona and Centro Nacional de

Investagionves Oncológicas (CNIO) INVENTORS: Mª Antonia Blasco, Bruno Bernardes, Fàtima Bosch and

Eduard Ayuso. REQUEST NO: EP10168341.5 PRIORITY COUNTRY: Europe PRIORITY DATE: 2 July 2010 EXTENDED TO COUNTRIES: COMPANIES EXPLOITING IT: TITLE: Gene therapy composition for use in diabetes treatment. HOLDER: Universidad Autonoma de Barcelona INVENTORS: Fàtima Bosch, Eduard Ayuso and David Callejas. REQUEST NO: EP10169309.1 PRIORITY COUNTRY: Europe PRIORITY DATE: 12 July 2010 EXTENDED TO COUNTRIES: COMPANIES EXPLOITING IT: Under discussion

TUTOR OF PhD AND MASTER THESIS

1.Title: CLONING, AMPLIFICATION AND TITRATION OF HELPER-DEPENDENT ADENOVIRAL VECTORS USING CRE66 CELL LINE AND A FLUORESCENT REPORTER GENE (Master Thesis) (2008) Student: Eustacchio Attico University: Universita degli Studi di Parma, Italy Tutor: Eduard Ayuso and Elena Maestri 2.Title: IMAGE ANALYSIS IN FEED SAFETY AND MOLECULAR BIOLOGY IN ANIMAL MODELS (PhD thesis) (2009) Student: Luca Maggioni University: Universita degli Studi di Milano, Italy Tutor: Eduard Ayuso and Luciano Pinotti 3.Title: DEVELOPMENT OF TWO DIFFERENT TECHNIQUES FOR VISUALIZATION AND MONITORIZATION OF PANCREATIC B-CELLS IN VIVO (Master Thesis) (September 2010) Student: Meritxell Morro University: Universitat Autonoma de Barcelona, Spain Tutor: Eduard Ayuso and Fatima Bosch 4.Title: GENERATION OF PANCREATIC-SPECIFIC VECTORS FOR THE STUDY OF DIABETES (Master Thesis) (September 2011) Student: Serena Marletta University: Universita degli Studi di Parma, Italy Tutor: Eduard Ayuso and Alessio Peracchi 5.Title: PANCREATIC TRANSDUCTION BY HELPER-DEPENDENT ADENOVIRAL VECTORS VIA INTRADUCTAL DELIVERY (Master Thesis) (July 2012) Student: Ramona Kratzer University: Ulm University, Ulm, Germany Tutor: Eduard Ayuso and Florian Kreppel 6.-Title: REPROGRAMMING ACINAR TO BETA CELLS. (PhD thesis) (ongoing) Student: Joan Teichenne. University: Universitat Autonoma de Barcelona, Spain. Tutor: Eduard Ayuso. 7.- Title: GENETIC MANIPULATION OF THE PANCREAS FOR THE STUDY OF DIABETES MELLITUS (PhD Thesis) (May 2014) Student: Meritxell Morro University: Universitat Autonoma de Barcelona, Spain Tutor: Eduard Ayuso and Fatima Bosch 8.- Title: GENETIC MANIPULATION OF THE PANCREAS FOR THE STUDY OF DIABETES MELLITUS (Ph Thesis) (ongoing) Student: Magalie Penaud-Budloo University: University of Nantes Tutor: Eduard Ayuso and Philippe Mouller INVITED REVIEWER Since 2014.- BMC Biotechnology Since 2011. Diabetologia Since 2011. Plos One

Since 2010. Molecular Therapy. Since 2010. Grant Reviewer for the AFM (French Association against Myopaties) Since 2008. Journal of Virological Methods Since 2006. Human Gene Therapy. Since 2006. Journal of Gene Medicine. EDITOR Since 2013. Academic editor for Plos One.

PUBLICATIONS Eduard Ayuso, Véronique Blouin, Martin Lock, Susan McGorray, Xavier Leon, Mauricio R. Alvira, Alberto Auricchio, Stephanie Bucher, Abdelwahed Chtarto, K. Reed Clark, Christophe Darmon, Monica Doria, Will Fountain, Guangping Gao, Kai Gao, Mauro Giacca, Juergen Kleinschmidt, Barbara Leuchs, Catherine Melas, Hiroaki Mizukami, Marcus Müller, Yvet Noordman, Olivier Bockstael, Keiya Ozawa, Catherine Pythoud, Marina Sumaroka, Richard Surosky, Liliane Tenenbaum, Inge van der Linden, Brigitte Weins, J. Fraser Wright, Xinhua Zhang, Lorena Zentilin, Fatima Bosch, Richard O Snyder and Philippe Moullier. MANUFACTURING AND CHARACTERIZATION OF A RECOMBINANT ADENO-ASSOCIATED VIRUS TYPE 8 REFERENCE STANDARD MATERIAL (SUBMITTED) 1.-M. Morro, J. Teichenne, V. Jimenez, R. Kratzer, S. Marletta, L. Maggioni, J. Ruberte, F. Kreppel, S. Kochanek, F. Bosch, E. Ayuso*. PANCREATIC TRANSDUCTION BY HELPER-DEPENDENT ADENOVIRAL VECTORS VIA INTRADUCTAL DELIVERY. Human Gen Ther (submitted revised version). * corresponding author. 2.- V. Haurigot, S. Marcó, A. Ribera, M. García, A. Ruzo, P. Villacampa, E. Ayuso, S. Añor, A. Andaluz, M. Pineda, G. García Fructoso, M. Molas, L. Maggioni, S. Muñoz, S. Motas, J. Ruberte, F. Mingozzi, M. Pumarola, and F. Bosch. WHOLE BODY CORRECTION OF MUCOPOLYSACCHARIDOSIS IIIA BY INTRACEREBROSPINAL FLUID GENE THERAPY. Journal of Clinical Investigation 2013 Jul 1. pii: 66778. doi: 10.1172/JCI66778 3.-Callejas D*, Mann CJ*, Ayuso E, Lage R, Grifoll I, Roca C, Andaluz A, Ruiz-de Gopegui R, Montane J, Munoz S, Ferre T, Haurigot V, Zhou S, Ruberte J, Mingozzi F, High K, Garcia F, Bosch F. TREATMENT OF DIABETES AND LONG-TERM SURVIVAL FOLLOWING INSULIN AND GLUCOKINASE GENE THERAPY. Diabetes. 2013 Feb 1. [Epub ahead of print]. * co-first authors 4.-E. Ayuso. GENE THERAPY FOR DIABETES (Teràpia gènica de la diabetes mellitus). Diabetes Avui. 2013. Epub (http://www.acdiabetis.org/documentacio.php). 5.-José A, Sobrevals L, Camacho-Sánchez JM, Huch M, Andreu N, Ayuso E, Navarro P, Alemany R, Fillat C. INTRADUCTAL DELIVERY OF ADENOVIRUSES TARGETS PANCREATIC TUMORS IN TRANSGENIC ELA-MYC MICE AND ORTHOTOPIC XENOGRAFTS. Oncotarget. 2013 [Epub ahead of print] 6.- Guegan T, Cutando L, Ayuso E, Santini E, Fisone G, Bosch F, Martinez A, Valjent E, Maldonado R, Martin M. OPERANT BEHAVIOR TO OBTAIN PALATABLE FOOD MODIFIES NEURONAL PLASTICITY IN THE BRAIN REWARD CIRCUIT.EUR NEUROPSYCHOPHARMACOL. Eur Neuropsychopharmacol. 2013 Feb;23(2):146-59

7.- L. Fernández-Sánchez, P. Lax, C. Isiegas, E. Ayuso, J. M. Ruiz, P. de la Villa, F. Bosch, E. J. de la Rosa and N. Cuenca. PROINSULIN SLOWS RETINAL DEGENERATION AND VISION LOSS IN THE P23H RAT MODEL OF RETINITIS PIGMENTOSA. Hum Gene Ther. 2012 Dec; 23(12):1290-300. 8.- Ruzo A, Marco S, Garcia M, Villacampa P, Ribera A, Ayuso E, Maggioni L, Mingozzi F, Haurigot V, Bosch F. CORRECTION OF PATHOLOGICAL ACCUMULATION OF GLYCOSAMINOGLYCANS IN CNS AND PERIPHERAL TISSUES OF MPSIIIA MICE THROUGH SYSTEMIC AAV9 GENE TRANSFER. Hum Gene Ther. 2012 Dec;23(12):1237-46. 9.- J. Agudo, E. Ayuso, V. Jimenez, A. Casellas, C. Mallol, A. Salavert, S. Tafuro, M. Obach, A. Ruzo, M. Moya, A. Pujol, and F. Bosch. VEGF-MEDIATED ISLET HYPERVASCULARIZATION AND INFLAMMATION CONTRIBUTE TO PROGRESSIVE REDUCTION OF Β-CELL MASS. Diabetes. 2012 Nov;61(11):2851-61. 10.- M. Oosterveer, C. Mataki, H. Yamamoto, T. Harach, No. Moullan, T. van Dijk, E. Ayuso, F. Bosch, C. Postic, A. Groen, J. Auwerx, and K. Schoonjans. LRH-1-DEPENDENT GLUCOSE SENSING DETERMINES THE FATE OF INTERMEDIARY METABOLISM IN MOUSE LIVER. Journal of Clinical Investigation 2012 Aug 1;122(8):2817-26. 11.- Bernardes de Jesus B, Vera E, Schneeberger K, Tejera AM, Ayuso E, Bosch F, Blasco MA. TELOMERASE GENE THERAPY IN ADULT AND OLD MICE DELAYS AGING AND INCREASES LONGEVITY WITHOUT INCREASING CANCER. EMBO Mol Med. 2012 Aug;4(8):691-704. 12.- E. Ayuso*, V. Blouin, C. Darmon, F. Bosch, M. Lock, R. Snyder, P. Moullier. REFERENCE MATERIALS FOR THE CHARACTERIZATION OF ADENO-ASSOCIATED VIRAL VECTORS. Book chapter in The CliniBook: Clinical gene transfer. Edited by Odile Cohen-Haguenauer – EDK, Paris © 2012 *corresponding author 13.- E. Ayuso, D. Callejas, C. Mann, V. Jimenez, F. Bosch. AAV GENE THERAPY FOR DIABETES MELLITUS. Book chapter in The CliniBook: Clinical gene transfer. Edited by Odile Cohen-Haguenauer – EDK, Paris © 2012 14.- E. Ayuso, F. Bosch. HIGHLIGHTS ON AAV MEDIATED GENE TRANSFER: INTRODUCTION. Book chapter in The CliniBook: Clinical gene transfer. Edited by Odile Cohen-Haguenauer – EDK, Paris © 2012 15.- Ruzo A, Garcia M, Ribera A, Villacampa P, Haurigot V, Marcó S, Ayuso E, Anguela XM, Roca C, Agudo J, Ramos D, Ruberte J, Bosch F. LIVER PRODUCTION OF SULFAMIDASE REVERSES PERIPHERAL AND AMELIORATES CNS PATHOLOGY IN MUCOPOLYSACCHARIDOSIS IIIA MICE. Mol Ther. 2012 Feb;20(2):254-66.

16.- V. Jimenez, E. Ayuso, C. Mallol, J. Agudo, A. Casellas, M. Obach, S. Muñoz, A. Salavert & F. Bosch. IN VIVO GENETIC ENGINEERING OF PANCREATIC BETA CELLS MEDIATED BY SINGLE-STRANDED ADENO-ASSOCIATED VIRAL VECTORS OF SEROTYPES 6, 8 AND 9. Diabetologia. (2011). 54:1075–1086 17.- Lock M, McGorray S, Auricchio A, Ayuso E, Beecham EJ, Blouin-Tavel V, Bosch F, Bose M, Byrne BJ, Caton T, Chiorini JA, Chtarto A, Clark KR, Conlon T, Darmon C, Doria M, Douar A, Flotte TR, Francis JD, Francois A, Giacca M, Korn MT, Korytov I, Leon X, Leuchs B, Lux G, Melas C, Mizukami H, Moullier P, Müller M, Ozawa K, Philipsberg T, Poulard K, Raupp C, Rivière C, Roosendaal SD, Samulski RJ, Soltys SM, Surosky R, Tenenbaum L, Thomas DL, van Montfort B, Veres G, Wright JF, Xu Y, Zelenaia O, Zentilin L, Snyder RO. CHARACTERIZATION OF A RECOMBINANT ADENO-ASSOCIATED VIRUS TYPE 2 REFERENCE STANDARD MATERIAL. Hum Gene Ther. (2010) Oct;21(10):1273-85 18.- E. Ayuso, C. J. Mann, X. Anguela and F. Bosch. GENE THERAPY APPROACHES FOR TYPE 1 DIABETES. Avances en Diabetología (2010) 26(1):6-12 19.- E. Ayuso, F. Mingozzi and F. Bosch. PRODUCTION, PURIFICATION AND CHARACTERIZATION OF ADENO-ASSOCIATED VECTORS. Current Gene Therapy (2010) Dec; 10(6): 423-426. 20.- Tung YC, Ayuso E, Shan X, Bosch F, O'Rahilly S, Coll AP, Yeo GS. HYPOTHALAMIC-SPECIFIC MANIPULATION OF FTO, THE ORTHOLOG OF THE HUMAN OBESITY GENE FTO, AFFECTS FOOD INTAKE IN RATS. PLOS ONE. 2010 Jan 19;5(1):e8771. 21.- Mann CJ, Ayuso E, Anguela XM, Bosch F. Skeletal muscle metabolism in the pathology and treatment of type 1 diabetes. Curr Pharm Des. (2010);16(8):1002-20. 22.- Ayuso E, Mingozzi F, Montane J, Leon X, Anguela XM, Haurigot V, Edmonson SA, Africa L, Zhou S, High KA, Bosch F, Wright JF. HIGH AAV VECTOR PURITY RESULTS IN SEROTYPE- AND TISSUE-INDEPENDENT ENHANCEMENT OF TRANSDUCTION EFFICIENCY. Gene Ther. (2010) Apr;17(4):503-10. 23.- Nacher V, Carretero A, Navarro M, Ayuso E, Ramos D, Luppo M, Rodr√≠guez A, Mendes L, Herrero-Fresneda I, Ruberte J. ENDOTHELIAL CELL TRANSDUCTION IN PRIMARY CULTURES FROM REGRESSING MESONEPHROS. Cells Tissues Organs. (2010);191(2):84-95. 24.- Haurigot V, Villacampa P, Ribera A, Llombart C, Bosch A, Nacher V, Ramos D, Ayuso E, Segovia JC, Bueren JA, Ruberte J, Bosch F. INCREASED INTRAOCULAR INSULIN-LIKE GROWTH FACTOR-I TRIGGERS BLOOD-RETINAL BARRIER BREAKDOWN. J Biol Chem. (2009) Aug 21;284(34):22961-9.

25.- Sabrina Tafuro, Eduard Ayuso, Serena Zacchigna, Lorena Zentilin, Silvia Moimas, Franca Dore, Mauro Giacca. INDUCIBLE AAV VECTORS INDUCE FUNCTIONAL ANGIOGENESIS IN ADULT ORGANISMS VIA REGULATED VEGF EXPRESSION. Cardiovasc Res. (2009) Sep 1;83(4):663-71. 26.- J. Agudo*, E. Ayuso*, V. Jimenez, A. Salavert, A. Casellas, S. Tafuro, V. Haurigot, J. Ruberte, J.C. Segovia, J. Bueren and F. Bosch. IGF-I MEDIATES REGENERATION OF ENDOCRINE PANCREAS BY INCREASING BETA CELL REPLICATION THROUGH CELL CYCLE PROTEIN MODULATION. *Both authors contribute equally to this work. Diabetologia (2008) oct;51(10):1862-72. 27.- E. Fusi, A. Baldi, F. Cheli, R. Rebucci, E. Ayuso, K. Sejrsen, S. Purup. EFFECTS OF PUTRESCINE, CADAVERINE, SPERMINE, SPERMIDINE AND Β-PHENYLETHYLAMINE ON CULTURED BOVINE MAMMARY EPITHELIAL CELLS. Italian Journal of Animal Science (2008) vol. 7, 131-140. 28.- A. Casellas, A. Salavert, J. Agudo, E. Ayuso, V. Jimenez, M. Moya, S. Muñoz, S. Franckhauser & F. Bosch. EXPRESSION OF IGF-I IN PANCREATIC ISLETS PREVENTS LYMPHOCYTIC INFILTRATION AND PROTECTS MICE FROM TYPE 1 DIABETES. Diabetes (2006) Dec;55(12):3246-55. 29.- Nacher V., Carretero A, Navarro M, Armengol C, Llombart C, Rodriguez A, Herrero-Fresneda I, Ayuso E, Ruberte J.THE QUAIL MESONEPHROS: A NEW MODEL FOR RENAL SENESCENCE?. Journal of Vascular Research (2006). 43(6):581-6. 30.- E. Ayuso, M. Chillón, F. García, J. Agudo, A. Andaluz, A. Carretero, M. Monfar, M. Moya, J. Montane, P. J. Otaegui, F. Bosch. IN VIVO GENE TRANSFER TO HEALTHY AND DIABETIC CANINE PANCREAS. Molecular Therapy (2006) 13; 747-55. 31.- F. Bosch, S. Franckhauser, P. Otaegui, T. Ferre, A. Pujol, S. Muñoz, A. Mas, A. Hidalgo, E. Ayuso, E. Riu. TRANSGENIC OVEREXPRESION STUDIES IN OBESITY AND DIABETES. Progress in obesity Research: 9. Ed John Libbey Eurotext Ltd. 2003. Chapter 61. pp 288-292. 32.- E. Ayuso, M. Chillon, J. Agudo, V. Haurigot, A. Bosch, P. Otaegui and Fàtima Bosch. IN VIVO GENE TRANSFER TO PANCREATIC ß-CELLS BY SYSTEMIC DELIVERY OF ADENOVIRAL VECTORS. Human Gene Therapy. (2004) 15 (8), 805-812 33.- J. Ruberte*, E. Ayuso*, M. Navarro, A. Carretero, V. Nacher, V. Haurigot, M. George, C. Llombart, A. Casellas, C. Costa, A. Bosch and F. Bosch. INCREASED OCULAR LEVELS OF INSULIN-LIKE GROWTH FACTOR-I IN TRANSGENIC MICE LEAD TO DIABETIC-LIKE EYE DISEASE. Journal of Clinical Investigation. (2004) 113 (8), 149-1167. *Both authors contribute equally to this work. 34.- George M., Ayuso E., Casellas A., Costa C., Devedjian JC., Bosch F. B-CELL EXPRESSION OF INSULIN-LIKE GROWTH FACTOR-I LEADS TO

RECOVERY FROM TYPE 1 DIABETES. Journal of Clinical Investigation (2002). 109:1153-1163. 35.- Arcalís T., Carretero A., Navarro M., Ayuso E., Ruberte J. VASCULOGENESIS AND ANGIOGENESIS IN THE SUBCARDINAL VENOUS PLEXUS OF QUAIL MESONEPHROS: SPATIAL AND TEMPORAL MORPHOLOGICAL ANALYSIS. Anatomy and Embriology. (2002) 205:19-28.

ABSTRACTS -Cheli F., Ayuso E., Rossi L., Dell´Orto V. Biogenic amines affect MEC proliferation. 52 th European Association for Animal Production (EAAP) Congress. The Haye, Netherlands. (2000) -Casellas A., Ayuso E., Franckhauser S., Moya M., Riu E., Ferre T., Otaegui P.J., Bosch F. Animales transgénicos que expresan IFNß especificamente en células ß. Un nuevo modelo animal de diabetes tipo I. XXIII Congreso de de la Sociedad Española de Bioquímica y Biología Molecular (SEBBM). Granada, España. (2000) -Chillón M, Ayuso E., García F., Otaegui P., Riu E., Juanola B., Bosch F. Transferencia génica mediante vectores virales para dirigir la expresión de genes exógenos a islotes pancreáticos in vivo. I Reuniónde la Sociedad Española para la Terapia Génica (SETG). Valencia, España. (2001) -George M., Ayuso E., Casellas A., Costa C., Pareja J., Moya M., Devedjian J.C., Bosch F. La expresión del factor de crecimiento semejante a la insulina (IGF-I) específicamente en células ß contraresta la diabetes tipo 1. I Reunión SETG, Valencia, España. (2001) -Casellas A., Ayuso E., García M., Franckhauser S., Moya M., Pareja J., Pascual G., Bosch F. ratones transgénicos que expresan IFNß en células ß. Un modelo para el ensayo de nuevas terapias para la diabetes tipo 1. I Reunión SETG, Valencia, España. (2001) -Arcalís T., Navarro M., Carretero A., Ayuso E., Nacher V., Ruberte J. Embryonic vascular development of quail mesonephros: paracrine and autocrine VEGF production. 3 th Congress of Spanish Society for Developmental Biology. Septiembre. Málaga. (2001). -Llombart C., Navarro M., Carretero A., Nacher V., Melgarejo V., Ayuso E., Bosch F., Ruberte J. Non-degeneration of hyaloid artery during eye development of IGF-I transgenic mouse:a model to study the Persistent Hyperplastic Primary Vitreous syndrome. XXIV European Association of Veterinary Anatomists (EAVA). Brno. Txèquia. Juliol 2002. -Ruberte J., Ayuso E., Navarro M., Carretero A., George M., Nacher V., Costa C., Casellas A., Haurigot V., Bosch F. Transgenic mice overexpressing IGF-I in the eye: A new animal model to study diabetic eye disease. European Association for the Study of Diabetes (EASD). Budapest. 2002. -Ayuso E., Ruberte J., Navarro M., Carretero A., George M., Nacher V., Costa C., Casellas A., Haurigot V., Bosch A., Bosch F. Transgenic mice overexpressing IGF-I in the eye: A new animal model for diabetic eye disease. 10th Annual Meeting of the European Society of Gene Therapy. Antibes, France. 2002.

-E. Ayuso, M. Chillon, J. Agudo, V. Haurigot, A. Bosch, P. Otaegui, F. Bosch. Transferència gènica a cèl.lules ß pancreàtiques in vivo mijançant vectors adenovirals. 7è Congrés de l´Associació Catalana de Diabetis. Bellaterra 8-10 Maig. 2003.

-A. Casellas, A. Salavert, E. Ayuso, M. Moya, M. Garcia, S. Franckhauser, S. Muñoz, J. Agudo i F. Bosch. L’expressió d’IGF-I en les cèl·lules ß prevé la resposta inflamatòria en un ratolí transgènic model de diabetis tipus 1. 7è Congrés de l´Associació Catalana de Diabetis. Bellaterra 8-10 Maig. 2003.

-A. Salavert, A. Casellas, E. Ayuso, J. Agudo, M. George, F. Bosch. Ratolins transgènics que expressen IGF-II en les cèl·lules ß desenvolupen diabetis després del tractament amb streptozotocina. 7è Congrés de l´Associació Catalana de Diabetis. Bellaterra 8-10 Maig. 2003.

-E. Ayuso, M. Chillon, J. Agudo, V. Haurigot, A. Bosch, P. Otaegui and F. Bosch. In vivo gene transfer to pancreatic ß cells using adenoviral vectors. 18th International Diabetes Federation Congress. Paris. August. 2003. -A. Casellas, A. Salavert, E. Ayuso, M. Moya, M. Garcia, S. Franckhauser, S. Muñoz, J. Agudo and F. Bosch. ß-cell expression of IGF-I prevents inflammatory response in a transgenic mouse model of autoimmune diabetes. 18th International Diabetes Federation Congress. Paris. August. 2003.

-Salavert, A. Casellas, E. Ayuso, J. Agudo, M. George and F. Bosch. Transgenic mice expressing IGF-II in ß cells develop diabetes after streptozotocin treatment. 18th International Diabetes Federation Congress. Paris. August. 2003.

-E. Ayuso, M. Chillon, F. García, J. Agudo, V. Haurigot, A. Bosch, A. Andaluz, P. Otaegui, F. Bosch. Transferencia génica in vivo a páncreas exocrino y endocrino mediante vectores adenovirales. II Reunion de la Sociedad Española de Terapia Génica. Barcelona. Marzo. 2003. -A. Salavert, A. Casellas, E. Ayuso, M. Moya, M.Garcia, S. Franckhauser, S. Muñoz, J. Agudo y F. Bosch. Caracterización de un modelo transgénico de diabetes autoimmune. papel de la expresión pancreática de IGF-I en la prevención de la respuesta inflamatoria. II Reunion de la Sociedad Española de Terapia Génica. Barcelona. Marzo. 2003.

-J.Agudo, E. Ayuso, A. Salavert, A. Casellas, T. Ferrer, A. Pujol and F. Bosch. Generation of transgenic mice overexpressing vascular endothelial growth factor in ß-cells. I Jornada del Departament de Bioquímica I Biologia Molecular. Bellaterra. Octubre. 2003. -V. Haurigot, J. Ruberte, E. Ayuso, M. Navarro, A. Carretero, M. George, V. Nacher, C. Costa, A. Casellas, A. Bosch, F. Bosch. Ratolins transgenics que sobreexpresen

IGF-I a la retina desenvolupen malaltia ocular diabètica. 7è Congrés de l´Associació Catalana de Diabetis. Bellaterra 8-10 Maig. 2003. -F. Bosch, E. Ayuso, M. Navarro, A. Carretero, V. Nacher, V. Haurigot, M. George, C. Llombart, A. Casellas, C. Costa, A. Bosch, J. Ruberte. Increased ocular levels of insulin-like growth factor-I in transgenic mice lead to diabetic eye disease. Eumorphia First Open Annual Meeting. 6-8 October. Slough, UK. 2003. -F. Bosch, E. Ayuso, M. Navarro, A. Carretero, V. Nacher, V. Haurigot, M. George, C. Llombart, A. Casellas, C. Costa, A. Bosch, J. Ruberte. Ratones transgénicos con niveles de IGF-I incrementados en el ojo desarrollan enfermedad ocular diabética. XXVI Congreso de la SEBBM. A Coruña, 15-18 Septiembre. 2003 -J. Ruberte, E. Ayuso, M. Navarro, A. Carretero, V. Nacher, V. Haurigot, M. George, C. Llombart, A. Casellas, C. Costa, A. Bosch & F. Bosch. Vascular endothelial growth factor is overexpressed in retinal glial cells of insulin-like growth factor-I transgenic mice. Angiogenesis 2 (Euroconferences). Paris. June 19-20. 2003. -E. Ayuso, M. Chillón, F. García, J. Agudo, V. Haurigot, A. Bosch, A. Andaluz, P. J. Otaegui & F. Bosch. Transferencia génica a páncreas in vivo mediante vectores adenovirales. VIII Congreso de la Sociedad Española de Virología. Barcelona. Spain. 2003. -E. Ayuso, M. Chillón, F. García, J. Agudo, A. Andaluz V. Haurigot, A. Bosch, , P. J. Otaegui & F. Bosch. Adenovirus-mediated gene transfer to mouse and dog pancreas. 7th Annual Meeting of the American Society of Gene Therapy. Minneapolis. USA. 2004. -P. Otaegui, J. Agudo, E. Ayuso, A.Salavert, A.Casellas, T. Ferré, V. Jiménez, A. Pujol and F. Bosch. Generation of transgenic mice overexpressing vascular endothelial growth factor in ß-cells. XXVII Congreso SEBBM. Lleida 2004 -A. Salavert, A. Casellas, J. Agudo, E. Ayuso, M. George and F.Bosch. Transgenic mice expressing igf-ii in ß-cells develop diabetes after treptozotocin treatment. XXVII Congreso SEBBM. Lleida 2004. -Ruberte, J., Ayuso E., Carretero, A., Nacher, V., Haurigot, V., George, M., Llombart, C., Costa, C., Bosch, A., Bosch, F. El ratón transgénico con expresión de igf-1 en el ojo reproduce las alteraciones oculares de la diabetes humana. XXVII Congreso de la Sociedad Española de Bioquímica y Biología Molecular. 2004 -E. Ayuso, M. Chillón, F. García, J. Agudo, V. Haurigot, A. Bosch, PJ. Otaegui, F. Bosch. Adenovirus-mediated gene transfer to mouse and dog pancreas in vivo. XII annual congress of the ESGT. Tampere, Finland. 2004 -J. Agudo, X. Gregory, G. Pascual, E. Ayuso, A. Salavert, M. Garcia, G. Solanes, E. Riu, T. Ferré, A. Casellas, M. Moya and F. Bosch. Expression of igf-1 recovers ß-cell mass in transgenic mice overexpressing gk specifically in ß-cells. EUMORPHIA Annual Meeting. London, 2004

-E. Ayuso, V. Jimenez, J. Agudo, V. Haurigot, J.C. Segovia, J. Bueren and F. Bosch. Bone marrow-derived cells do not contribute to endocrine pancreas regeneration in diabetic RIP/IGF-I transgenic mice. 8th Annual Meeting of the American Society of Gene Therapy. Saint Louis. USA. 2005. -Sabrina Tafuro, Eduard Ayuso, Serena Zacchigna, Lorena Zentilin and Mauro Giacca. An inducible AAV-VEGF vector system defines the proper timing and dosage of VEGF expression required for the formation of stable and mature blood vessels. XIII Congress of the European Society of Gene Therapy. Prague. 2005. -Alba Casellas, Ariana Salavert, Judith Agudo, Eduard Ayuso, Veronica Jimenez, Marta Moya, Sergio Muñoz, Sylvie Franckhauser and Fatima Bosch. Expression of IGF-I in pancreatic islets prevents lymphocytic infiltration and protects mice from type 1 diabetes. Betacelltherapy Symposium. Brussels. January 2006. -Joel Montane, Eduard Ayuso, Xavier M. Anguela, David Callejas, Chris Mann, Federico Mingozzi, Katherin A. High, Fatima Bosch. Eficient transduction of canine liver with AAV-1 using peripheral vein high-pressure delivery. XIV Annual Congress of the European Society of Gene Therapy. Athens. November. 2006. -Eduard Ayuso, Judith Agudo, Veronica Jimenez,Ariana Salavert ,Virginia Haurigot, Sabrina Tafuro , Jesus Ruberte, Jose Carlos Segovia, Juan Bueren and Fatima Bosch. Endocrine pancreas regeneration in diabetic IGF-I transgenic mice results from replication of pre-existing ß-cells rather than from bone marrow cell recruitment and differentiation. Betacelltherapy Symposium. Brussels. January 2007. - Judith Agudo, Eduard Ayuso, Verónica Jimenez, Ariana Salavert, Virginia

Haurigot, Sabrina Tafuro, Jesús Ruberte, José Carlos Segovia, Juan Bueren, Fàtima Bosch. Endocrine pancreas regeneration in diabetic IGF-I transgenic mice results from replication of pre-existing ß-cells rather than from bone marrow cell recruitment and differentiation.. American Diabetes Association´s 67th Scientific Sessions. Chicago, USA, 22-26 Juny 2007.

-Eduard Ayuso, Joel Montane, Xavier M. Anguela, David Callejas, Chris Mann, Felix García, Anna Andaluz, Federico Mingozzi, Katherin A. High, Fatima Bosch. High-preassure delivery via peripheral vein facilitates transduction of canine liver with AAV vectors.10th Annual Meeting American Society of Gene Therapy. Seattle. June. 2007. -A. Filipe, E. Ayuso, JAAM Kamps JAAM, G. Molema, M. Giacca, MC de Lima MC, S. Simões. Targeted gene delivery to activated endothelial cells. XV Annual Congress of the European Society of Gene Therapy. Rotterdam. November. 2007. -Eduardo Ayuso , Joel Montane , Xavi M. Anguela , David Callejas, Christopher J. Mann, Ana Andaluz, Felix Garcia, Federico Mingozzi, Katherine A. High , Fatima Bosch. Incremento de la transducción hepática mediante inyección hidrodinámica de vectores adenoasociados. IV Congreso Sociedad Española de Terapia Génica y Celular. Madrid. Spain. 2007. - Eduardo Ayuso, Verónica Jiménez, Judith Agudo, Pedro J. Otaegui, Sabrina

Tafuro, Florian Kreppel, Stefan Kochanek, Fatima Bosch. Aplicaciones de vectores adenoasociados de serotipo 8 y de vectores adenovirales de tipo gutless para la transferencia de genes a páncreas. IV Congreso de la Sociedad Española de Terapia Génica y Celular (SETGyC). Facultad de Medicina, Universida Autónoma de Madrid, 19-21 Juny 2007.

-Eduard Ayuso, Joel Montane, Xavier M. Anguela, Chris Mann, David Callejas, Veronica Jimenez, Sabrina Tafuro, Ana Andaluz, Felix Garcia, Shangzhen Zhou, Federico Mingozzi, Katherine A. High and Fatima Bosch. Chemical and physical methods to achieve enhanced liver transduction by AAV1 vectors. Annual meeting of American Society of Gene Therapy. Boston. 2008. -Eduard Ayuso, Joel Montane, Federico Mingozzi, Xavier M. Anguela, Xavier León, Chris Mann, David Callejas, Veronica Jimenez, Sabrina Tafuro, Ana Andaluz , Felix Garcia, Shangzhen Zhou, Fraiser Wright, Katherine A. High and Fatima Bosch. Optimized vector purification and delivery methods to achieve enhanced liver transduction by AAV vectors. XVIth Annual Congress of the European Society of Gene and Cell Therapy. Brugge, Belgium. November. 2008. - Eduard Ayuso, Judith Agudo, Veronica Jimenez, Ariana Salavert, Alba Casellas,

Sabrina Tafuro, Virginia Haurigot, Jesus Ruberte, Jose Carlos Segovia, Juan Bueren

and Fatima Bosch. Regeneración del páncreas endocrino mediada por igf-i: incremento de la replicación de las células ß y modificaciones en las proteínas de ciclo celular. CIBERDEM Annual Training Course 2008. 1ª Reunión del área 3: disfunción, destrucción y regeneración del islote. Autoinmunidad. Bellaterra. 2008.

- J. Agudo, A. Salavert, E. Ayuso, V. Jiménez, A. Casellas, A. Ruzo, S. Tafuro, J.

Ruberte and Fatima Bosch. Papel del vegf y del endotelio vascular en la función y la viabilidad del pancreas endocrino. Ciberdem Annual Training Course 2008. 1ª Reunión del área 3: disfunción, destrucción y regeneración del islote. Autoinmunidad. Bellaterra. 2008.

- V. Jiménez, E. Ayuso, J. Agudo, A. Salavert, S. Muñoz, M. Moya, X. Leon and

Fatima Bosch. Transferencia de genes a páncreas in vivo mediante vectores adenoasociados. CIBERDEM Annual Training Course 2008. 1ª Reunión del área 3: disfunción, destrucción y regeneración del islote. Autoinmunidad. Bellaterra. 2008.

- J. Agudo, A. Salavert, A. Casellas, V.Jimenez, E. Ayuso and F. Bosch. IGF-II

expression in beta-cells induces susceptibility to develop diabetes. 69th Annual meeting of the American Diabetes Association. San Francisco, USA. June, 2008. (Póster)

- David Callejas, Christopher J. Mann, Joel Montane, Eduardo Ayuso, Xavier M.

Anguela, Marta Moya, Xavier Leon, Anna Andaluz ,Federico Mingozzi, Katherine A. High , Felix Garcia and Fatima Bosch. Gene therapy for diabetic hyperglycaemia by expressing insulin and glucokinase (gk) in skeletal muscle: pre-clinical studies in diabetic dogs. 4th Eugene 2 Training Course. Capri, September 29-30, 2008

- Eduard Ayuso, Joel Montane, Xavier M. Anguela, Chris Mann, David Callejas,

Veronica Jimenez, Sabrina Tafuro, Ana Andaluz , Felix Garcia, Shangzhen Zhou,

Federico Mingozzi, Katherine A. High and Fatima Bosch. Impact of different factors in aav-mediated liver transduction in vivo. Clinigene 2nd anniversary meeting. Annecy. France. 2008.

- Albert Ruzo, Miquel García, Virginia Haurigot, Antonella Chiandetti, Xavier M.

Anguela, Carles Roca, Sara Marcó, Xavier León, Eduard Ayuso, Fàtima Bosch. AAV-mediated sulfamidase expression in the liver prevents development of somatic alterations in mps iiia mice. 16th ESGCT Annual Congress of the European Society of Gene and Cell Therapy (ESGCT). 13-16 November 2008. Brugge, Belgium.

- Albert Ruzo, Miquel García, Virginia Haurigot, Antonella Chiandetti, Xavier M.

Anguela, Carles Roca, Xavier León, Eduard Ayuso, Fàtima Bosch. AAV-mediated sulphamidase expression in liver or skeletal muscle prevents development of somatic alterations in mps IIIa mice. 11th Annual Meeting of the American Society of Gene Therapy. May 28-June 1, 2008. Boston, Massachusetts, USA.

-Eduard Ayuso, Federico Mingozzi, Joel Montane, Xavier Leon, Xavier M. Anguela, Virginia Haurigot, Shyrie Edmonson, Shangzhen Zhou, Katherine A. High, J. Fraser Wright, Fatima Bosch. An optimized gradient purification method that results in enhanced AAV transduction in vivo. Annual Congress of the Spanish Society of Gene and Cell Therapy. Granada. Spain. 2009. -Verónica Jiménez, Eduardo Ayuso, Judith Agudo, Ariana Salavert, Sergio Muñoz,

Marta Moya, Xavier Leon, David Ramos, Jesús Ruberte and Fàtima Bosch. In vivo gene transfer to the pancreas using adeno-associated viral vectors. X Congrés de la Asociación Catalana de Diabetes, 2009

- V. Jiménez, E. Ayuso, J. Agudo. A. Salavert. S. Muñoz, M. Moya, X. León, J. Ruberte and F. Bosch. In vivo gene transfer to the pancreas using adenoassociated viral vectors. V Congreso de la Sociedad Española de Terapia Génica y Celular, Granada, 2009 - A. Ruzo, M. García, V. Haurigot, S. Marcó, P. Villacampa, A. Ribera, C. Roca, X. Anguela, X. León, E. Ayuso and F. Bosch. AAV-mediated sulfamidase expression in the liver prevents development of somatic alterations in mps IIIa mice. V Congreso de la Sociedad Española de Terapia Génica y Celular, Granada, 2009. - Judith Agudo, Gemma Pascual, Xavier Gregori, Miquel Garcia, Eduard Ayuso,

Ariana Salavert, Alba Casellas, Verónica Jiménez, Pedro J. Otaegui and Fàtima Bosch. Expression of IGF-1 recovers ß-cell mass in transgenic mice overexpressing gk specifically in ß-cells. 2nd CIBERDEM Annual Meeting, Arnes, 26-28 octubre 2009

- Verónica Jiménez, Sergio Muñoz, Eduard Ayuso, Xavier Leon, Ivet Elias, Tura Ferre, Sylvie Franckhauser and Fàtima Bosch. Efficient gene transfer to white adipose tissue using adeno-associated viral vectors. V Congreso de la Sociedad Española de Terapia Génica y Celular, Granada, 2009

- A. José, M. Huch, N. Andreu, E. Ayuso, M. Moreno, P. Navarro and C. Fillat. optimization of tk/gcv suicide gene therapy for pancreatic cancer in tgela1-myc

mice. V Congreso de la Sociedad Española de Terapia Génica y Celular, Granada, 2009

- C. Isiegas, M. Marchena, E. Ayuso, F. Bosch, P. de la Villa, E.J. de la Rosa. Systemic delivery of human proinsulin by aav delays retinal degeneration in mouse models of retinitis pigmentosa. V Congreso de la Sociedad Española de Terapia Génica y Celular. Granada, 30 setembre-2 octubre 2009.

- Alba Casellas, Ariana Salavert, Judith Agudo, Mercè Obach, Verónica Jimenez, Eduard Ayuso and Fàtima Bosch. IGF-II expression in beta-cells induces susceptibility to develop diabetes. 2nd CIBERDEM Annual Meeting, Arnes, 26-28 octubre 2009

- Verónica Jiménez, Sergio Muñoz, Eduard Ayuso, Xavier Leon, Ivet Elias, Tura Ferre, Sylvie Franckhauser and Fàtima Bosch. Efficient gene transfer to white adipose tissue using adeno-associated viral vectors. European Society of Gene and Cell Therapy, Hanover, November 2009

- Judith Agudo, Eduard Ayuso, Ariana Salavert, Veronica Jimenez, Sabrina Tafuro, Alba Casellas, Mercè Obach, Albert Ruzo, Marta Moya, Xavier M. Anguela, Jesus Ruberte, and Fatima Bosch. increased vegf expression in ß-cells leads to islet inflammation and diabetes. 45th EASD Annual Meeting, Viena, 30 Sept-02 Oct 09

- Alba Casellas, Ariana Salavert, Judith Agudo, Mercè Obach, Verónica Jimenez,

Eduard Ayuso and Fàtima Bosch. IGF-II Expression in beta-cells induces susceptibility to develop diabetes. 45th EASD Annual Meeting, Viena, 30 Sept-02 Oct 09

- Carolina Isiegas, Miguel Marchena, Eduard Ayuso, Violeta Gómez-Vicente, Román Blanco, Flora de Pablo, Fátima Bosch, Pedro de la Villa and Enrique J. de la Rosa. Direct intravitreal administration and systemic aav-mediated delivery of proinsulin prevents retinal degeneration in the rd10 mouse model of retinitis pigmentosa. Association for Research in Vision and Ophthalmology. Annual meeting, 2009. Fort Lauderdale, Florida, USA.

-E. Ayuso, V. Jimenez , J. Agudo, A. Salavert, S. Muñoz, A. Casellas, M. Obach, M. Molas, M. Moya, X. Leon, J. Ruberte, and F. Bosch. Efficient gene transfer to the pancreas using ssAAV for prevention of Type 1 diabetes. Annual meeting of American Society of Gene Therapy. Washington DC. 2010. - D. Callejas, C.J. Mann, J. Montane, E. Ayuso, X. Leon, A. Andaluz, F. Mingozzi,

K.A. High, F. Bosch. gene therapy for diabetic hyperglycaemia by expressing insulin and glucokinase in skeletal muscle: pre-clinical studies in diabetic dogs. 3rd CIBERDEM Annual Meeting. Prullans (España), 25-27 octubre del 2010

- A Ruzo, M. Garcia, S Marcó, P. Villacampa, A. Ribera, E. Ayuso, V. Haurigot, J.

Ruberte, F. Bosch. AAV9-based sulfamidase gene transfer reverses somatic and

neurological pathology in mps IIIa mice. XVIII Annual Congress of the European Society of Gene and Cell Therapy (ESGCT). Milán, Italia, Octubre 2010

- Alba Casellas, Verónica Jiménez, Eduard Ayuso, Judith Agudo, Mercè Obach,

Ariana Salavert, Sergio Muñoz, Marta Moya, Xavier León, Maria Molas, David Ramos, Jesús Ruberte y Fàtima Bosch. Efficient gene transfer to the pancreas using adeno-associated viral vectors for prevention of type 1 diabetes in mice. 46º Congreso Anual de la Asociacion Europea para el Estudio de la Diabetes (EASD). Estocolmo (Suecia), 2010

- Verónica Jiménez, Sergio Muñoz, Eduard Ayuso, Xavier Leon, Ivet Elias, Tura

Ferre, Sylvie Franckhauser y Fàtima Bosch. Efficient gene transfer to white adipose tissue using adeno-associated viral vectors. CLINIGENE, 4º aniversary Annecy (Francia), 2010

- E. Ayuso, V. Blouin, C. Darmon, X. Leon, R. Snyder, F. Bosch, P. Moullier. The manufacture of an AAV8 reference standard material. Clinigene meeting. Paris. France. 2011. -Meritxell Morro, Ricardo Lage, Alba Casellas, Veronica Jimenez, Cristina Mallol, Serena Marletta, Joan Teichenne, Xavier Leon, Maria Molas, Florian Kreppel, Stefan Kochanek, Fatima Bosch and Eduard Ayuso*. *corresponding author. Non-invasive monitoring of insulin-producing cells in vivo with viral vectors. Annual meeting of the American Society of Gene of Cell Therapy. Philadelphia. USA. 2012 -Meritxell Morro, Ricardo Lage, Alba Casellas, Veronica Jimenez, Cristina Mallol, Serena Marletta, Joan Teichenne, Xavier Leon, Maria Molas, Florian Kreppel, Stefan Kochanek, Fatima Bosch and Eduard Ayuso*. *corresponding author. Non-invasive monitoring of pancreas transduction and β-cell mass in vivo with viral vectors. Annual meeting of the European Association for the Study of Diabetes (EASD). Berlin. Germany. 2012 F. Bosch, S. Marco, A. Ribera, M. Garcia, P. Villacampa, S. Motas, C. Roca, E. Ayuso, L. Maggioni, S. Anor, A. Andaluz, J. Ruberte, M. Pumarola, F. Mingozzi, and V. Haurigot. Towards a Gene Therapy for Neurological and Somatic MPSIIIA. European Society for cell and Gene Therapy. Madrid. 2013 S. Marco, A. Ribera, M. Garcia, P. Villacampa, S. Motas, C. Roca, E. Ayuso, L. Maggioni, S. Anor, A. Andaluz, J. Ruberte, V. Haurigot, F. Bosch. Long-term efficacy following intra-CSF administration of AAV9-sulfamidase in the treatment of MPSIIIA. European Society for cell and Gene Therapy. Madrid. 2013 Véronique Blouin, Aurelien Jacob, Lucie Menard, Achille François, Cécile Robin, Lionel Galibert, Otto-Wilhelm Merten, Dominique Aubert, Christophe Cheve, Philippe Moullier, Eduard Ayuso1,2. *corresponding author. Scalable upstream and downstream process for AAVrh10 vectors produced with baculovirus vectors in insect cells. Annual Meeting Amercian Society of Gene and Cell Therapy. Washington DC. USA. May 2014.

PARTICIPATION IN COURSES 2007- Cold Spring Harbor Laboratory. In vivo Barriers for Gene delivery. NY. USA. 2006.- EMBO Course. Programming pancreatic ß-cells. El perello. Spain. 2001.- Theoretical and practical course in small animal anesthesia. Barcelona, Spain. 2000.-Workshop Real time PCR: Identification of Genetically Modified Organisms (GMO). Monza , Italy. 1998.-XXII Animal nutrition course. Barcelona, Spain.