Rudolf Valenta

Div. of Immunopathology

Department of Pathophysiology

Center of Pathophysiology, Infectiology & Immunology

Medical University of Vienna, Austria

Nahrungsmittelallergien

www.oegai.org

www.meduniwien.ac.at/

allergy-research-program

www.meduniwien.ac.at/

allergy-research-christian-doppler

Adverse reactions to food

Toxic Non Toxic

Non Immune Mediated Immune Mediated

Enzymatic

Pharmacological

Undefined

IgE

Mixed

Non IgE

Classification of food intolerance

Clinical manifestations of adverse food reactions

1. Skin reactionsContact urticaria

Urticaria / angioedema

Flush, pruritus

Oral allergy syndrome (local itching and tingling and/or edema of lips,

tongue, palate and pharynx)

Protein contact dermatitis

Atopic eczema

2. Reactions of respiratory systemRhinoconjunctivitis

Asthma bronchiale

Laryngeal and/or pharyngeal edema

Hoarseness

3. Reactions of the gastrointestinal tractAbdominal pain

Abdominal distension

Nausea, vomiting

Gastritis

Gastroenteritis with diarrhea

Anorexia

Flatulence

Colitis

4. Reactions of the cardiovascular

systemCardiac dysarhythmia

Hypotension

Vascular collaps

Anaphylactic shock

4. Reactions of the cardiovascular

systemCardiac dysarhythmia

Hypotension

Vascular collaps

Anaphylactic shock

Urticaria

Angioedema

Atopic Dermatitis

Mechanisms of allergic inflammation

Larche M, et al., Nat Rev Immunol 2006, 6:761-71

Valenta R, et al., Adv Immunol 2004, 82:105-53

Diagnosis of type I allergy

Provocation testing Detection of allergen-

specific IgE antibodies

ALLERGEN EXTRACTS

Allergen

MC

Allergen source

Definition: Allergen source, allergen extract, allergen

Mixture of allergenic

and non-allergenic

components

Allergen extract

Disease-eliciting

molecule

Definition and production of recombinant allergens

DNA sequence

analysis

Expression of the

recombinant

allergen

IgE Immunoscreening

Isolation of

Phage DNA

cloning into a

vector

Isolation of the mRNA

Synthesis of cDNA

Phage library

Infection of E.coli

Allergen source (e.g., pollen,

animal dander, house dust mite....)

Serum IgE

panel of recombinant

allergens

Three-dimensional structures of important allergens

7 8 9 10 11 12

13 14 15 16 17 18

1 2 3 4 5 6

19 20 21 22 23 24

25 26 27 28 29 30

Bet v 1, the major birch pollen allergen:

Predictor for tree pollen (e.g., birch alder, hazel,

hornbeam, oak) and plant food sensitization

apple: Mal d 1

cherry: Pru av 1

alder: Aln g 1

hazel: Cor a 1

hornbeam: Car b 1

celery: Api g 1

carrot: Dau c 1

Kazemi-Shirazi L, et al., Int Arch Allergy Immunol.2002;127:259-68

Reading the IgE reactivity profiles of allergic patients

for the better managment

of allergic diseases

Hiller R, et al., FASEB J. 2002;16:414-6

Skin testing versus chip testing

Skin testing versus chip testing

Recombinant fish parvalbumin for diagnosis

of fish allergy

Reduced allergenic activity of the parvalbumin mutants

Hypoallergenic food allergens for

toleranceinduction and vaccination

Acknowledgements

Thank you for your attention!

Rudolf Valenta