Edwin J. Alvarado

José Antonio Cruz-Arzón

Environmental Data “Sample 3”

Date: 2013 March 4th 6:15 A.M.

Data: Location: 18.17336, –65.86055

Temperature: 25 ºC

Depth: 2.5 cm

Moisture: Dry

Geography: Rural Area

Water Proximity

Enrichment & Streaking Protocol

Date: 2013 March 5th Date: 2013 March 11th

Plaque Purification

Plaque Purification I

PP 1.1

PP 1.2

Date: 2013 April 3rd

Plaque Purification

Plaque Purification II

Plaque Purification I

PP 1.1

PP 1.2

PP 2.1.2

PP 2.2.1

PP 2.2.2

Date: 2013 April 4th

Preliminary Results & Conclusion

Results include two successful rounds of purification.

Third purification to be carried out in the coming weeks.

Positive soil samples seem to derive from areas with

dark soil and a high plant density.