^Murder mayhem and machines - CORE · 6.00 Stephanie2 #253 [modified by Administrator] WK 129...
Transcript of ^Murder mayhem and machines - CORE · 6.00 Stephanie2 #253 [modified by Administrator] WK 129...
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“Murder mayhem and machines”
Giorgio Blom
Alison Davidson
Jamie Pringle
John Cassella
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• Last year in the United Kingdom approximately 200,000 people went missing of which 5,500 of those cases had a fatal outcome, which can range from suicide to accidents and misadventure to violent crimes.
• How you find those persons who have been murdered and placed in shallow, hidden, clandestine graves is the main topic of this presentation
• The use of laboratory scientific techniques and how they move into the ‘real-world’ will demonstrate the power and impact of science on our society
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• The use of chemical analysis, in addition to other search methods ranging from Victim Recovery Dogs to ground penetrating radar, in order to locate human remains has found its way into the American juridical system, although the research and methodology were not ready at the time. This indicates that chemistry has a potential to aid in the detection of human remains, although this area of research still needs further exploration
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Where to start ?
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Soil Forensic Science Lorna Dawson video
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The officers, in Metropolitan Police uniform, searched the grass on their hands and knees
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• Current procedures to detect clandestine graves
• Limitations of these procedures
• Ideal procedure to detect clandestine graves
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No grant funding available for this
research
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Current techniques employed
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Mobile Technologytracking …..
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Current techniques employed
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A fast method to locate a body in a large area of earth…..
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• Four main clandestine burial decompositional stages. (A) Recent burial, surface expression is most obvious. (B) Early decomposition with search dogs and/or methane probes being optimal. (C) Late-stage decomposition with grave soil fluids. (D) Final skeletonised decomposition, GPR may locate.
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Is there a problem ?A famous case driven in part by the Media
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An aerial image shows the ground where police first started digging in the Madeleine McCann investigation yards from the apartment where she was last seen
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Metropolitan Police – news video- latest search for Madeline McCann
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If only murders would stand there and wait to be caught…….
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BBC TV COUNTRYFILE video
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• Putrescine and cadaverine are significantdecomposition products
• Putrescine and cadaverine are potentialbiomarkers for analytical instrument detectionof clandestine graves
• Prominent researchers did not detect thesecompounds in grave headspace or soil – why?
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Video on How GCMS works
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Giorgio video
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Analysis of leachate samples
Detection of putrescine and cadaverine the leachate samples over time.
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ION CHROMATOGRAPHY - AN EXAMINATION OF PORCINE LEACHATE
TO IDENTIFY CHEMICAL MARKERS FOR CLANDESTINE GRAVES
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Summary of the early Putrescine , Cadaverine and work
• Putrescine and cadaverine were detected in theleachate samples from 181 days up to 902 dayspost burial by GC-FID.
• Methylamine was also detected around this timeinterval.
• There is no linear relationship for theconcentration of putrescine and cadaverine overtime.
• Putrescine and cadaverine are less volatile ifdissolved in water – so they will hang aroundlonger in soil.
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ANALYTICAL METHODS (1)GC-MS CRANFIELD LEACHATE
, 23-Apr-2014 + 01:09:53
2.59 4.59 6.59 8.59 10.59 12.59 14.59 16.59 18.59 20.59 22.59 24.59 26.59 28.59 30.59 32.59 34.59Time0
100
%
CFG260911 220414 220414 Scan EI+ TIC
4.46e109.06
7.71
6.08
11.39
9.86
12.10
16.24
13.86
14.70
15.49
17.48
18.54
Total Ion Chromatogram:
Grave sample 5 weeks post
burial
, 23-Apr-2014 + 01:09:53
2.59 4.59 6.59 8.59 10.59 12.59 14.59 16.59 18.59 20.59 22.59 24.59 26.59 28.59 30.59 32.59 34.59Time0
100
%
CFG260911 220414 220414 Scan EI+ 208+249+263
1.92e97.64
17.48
12.10
10.12
9.58
11.18
16.96
13.86
20.2818.54
20.89
Reconstructed Ion Chromatogram:
Grave sample 5 weeks post burial
, 23-Apr-2014 + 02:49:28
2.59 4.59 6.59 8.59 10.59 12.59 14.59 16.59 18.59 20.59 22.59 24.59 26.59 28.59 30.59 32.59 34.59Time0
100
%
CFC260911 220414 220414 Scan EI+ TIC
4.35e109.04
6.09
7.65
11.73
Total Ion Chromatogram:
Control sample 5 weeks post burial
Putrescine
Cadaverine
Methylamine
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ANALYTICAL METHODS (1)GAS CHROMATOGRAPHY MASS SPECTROMETRY (GC-MS)
• Why?– Analysis of decomposition markers putrescine & cadaverine
• Methodology– Derivatisation of amines using pentafluorobenzaldehyde– DB-5MS column
• Outcomes– Methylamine another potential decomposition marker– RSD < 5% – Methylamine LOD(TIC) 4.2 ppb, LOQ(TIC) 12.6 ppb– Putrescine LOD(TIC) 15.9 ppb, LOQ(TIC) 48.3 ppb– Cadaverine LOD(TIC) 13.6 ppb, LOQ(TIC) 41.3 ppb(
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ANALYTICAL METHODS (2)ION CHROMATOGRAPHY (IC)
• Why?– Pringle 2012 demonstrated high levels of soil-water conductivity relating to the
gravesite samples
• Methodology– AS22 Column, Carbonate / bicarbonate Eluent, H2SO4 suppressor
• Outcomes– Fluoride & Acetate co-elute– RSD < 2% – Acetate LOD(TIC) 5.9ppm, LOQ(TIC) 18.2 ppm– Chloride LOD(TIC) 1.8 ppm, LOQ(TIC) 10.4 ppm– Nitrate LOD(TIC) 0.6 ppm, LOQ(TIC) 1.7 ppm– Phosphate LOD(TIC) 1.4 ppm, LOQ(TIC) 4.9 ppm– Carbonate LOD(TIC) 0.5 ppm, LOQ(TIC) 1.5 ppm
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ANALYTICAL METHODS (2)IC KEELE LEACHATE
0.0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0 11.3 12.5 13.8 15.0 16.3 17.5 18.8 20.0 21.3 23.0
-5.0
12.5
25.0
37.5
55.0Stephanie2 #255 [modified by Administrator] WK 129 (27/05/10) GS 18/12/2013 ECD_1µS
min
1 - 3.447
2 - 4.4603 - 4.707
4 - 7.213
5 - 8.953
6 - 10.263
7 - 11.870 8 - 13.147
Courtesy of Stephanie van Rens
0.0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0 11.3 12.5 13.8 15.0 16.3 17.5 18.8 20.0 21.3 23.0
-1.00
1.25
2.50
3.75
6.00Stephanie2 #253 [modified by Administrator] WK 129 (27/05/10) CS 18/12/2013 ECD_1µS
min
1 - 4.4532 - 6.750
3 - 8.453
4 - 9.703
Grave sample
129 weeks post burial
Control sample
129 weeks post burial
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ANALYTICAL METHODS (2)ION CHROMATOGRAPHY (IC)
• Why?– Pringle 2012 demonstrated high levels of soil-water conductivity relating to the
gravesite samples
• Methodology– AS22 Column, Carbonate / bicarbonate Eluent, H2SO4 suppressor
• Outcomes– Fluoride & Acetate co-elute– RSD < 2% – Acetate LOD(TIC) 5.9ppm, LOQ(TIC) 18.2 ppm– Chloride LOD(TIC) 1.8 ppm, LOQ(TIC) 10.4 ppm– Nitrate LOD(TIC) 0.6 ppm, LOQ(TIC) 1.7 ppm– Phosphate LOD(TIC) 1.4 ppm, LOQ(TIC) 4.9 ppm– Carbonate LOD(TIC) 0.5 ppm, LOQ(TIC) 1.5 ppm
Courtesy of Max Krens &
Stephanie van Rens
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CASEWORK SAMPLES A (WATER) IC
Courtesy of Vincent Voorwerk
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CASEWORK SAMPLES A (WATER) GC-MS
Courtesy of Vincent Voorwerk
, 28-Jul-2015 + 19:02:58
2.59 4.59 6.59 8.59 10.59 12.59 14.59 16.59 18.59 20.59 22.59 24.59 26.59 28.59 30.59 32.59 34.59Time0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
Positive control 280715 Scan EI+ 208+249+263
1.43e917.52
7.68
18.04 19.95
Sample A 280715 Scan EI+ 208+249+263
6.10e64.41
3.83
7.68
6.13
9.22
8.11 11.13 12.75 13.74
Sample B 280715 Scan EI+ 208+249+263
6.11e64.42
3.14
7.68
6.16
9.22
8.54 9.88 11.14 13.76
Sample C 280715 Scan EI+ 208+249+263
4.86e64.36
3.77
7.68
6.16
9.17
9.88 11.15 12.76
Control 1 280715 Scan EI+ 208+249+263
5.69e64.41
4.01
8.54
7.689.87
11.14 12.76 13.75
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CASEWORK SAMPLES (2)LOCATION B
• Sample type: Soil
• Victim went missing 15 years ago
• 12 sampling locations:
– 1 at body location
– 3 downslope (North)
– 2 upslope near dog indication (South)
– 3 further upslope (including control)
– 2 samples adjacent to the body (East & West)
• Samples taken from top and bottom of auger
– 24 samples including control samples
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SOIL EXTRACTION METHODOLOGY
1a. (IC) Dry 1g soil in oven at 60°C overnight or
1b. (GC) Mix 1g soil with 1g anhydrous sodium sulphate
2.Grind sample, sieve and place in centrifuge tube
3.Add 5ml distilled water and vortex sample for 10 seconds
4.Place samples in ultrasonic bath for 15 minutes, centrifuge for 10 minutes 2500rpm and filter aliquot
5.Re-do step 3 & 4 two times
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SOIL EXTRACTION RESULTS IC
Anion Spiked (mg·L-1) Found (mg·L-1) Control (mg·L-1) Recovery (%)RSD(%)
Fluoride 3.3245 0.9571 0.3005 19.75 3.0
Chloride 3.3245 0.9270 0.2457 20.49 2.4
Nitrite 3.3245 0.9419 0.4165 10.85 9.1
Nitrate 6.6490 5.9045 4.0984 17.16 7.9
Sulphate 3.3245 1.1875 0.4931 20.89 9.2
Courtesy of Vincent Voorwerk
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0
-2.0
2.5
5.0
7.5
12.0Vincent3 #142 Spiked A 5ml 08/05/15 ECD_1µS
min
1 - Fluoride - 3.463
2 - 4.140
3 - Chloride - 5.107
4 - Nitrite - 6.217
5 - Nitrate - 8.623
6 - Sulphate - 12.720
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CASEWORK SAMPLES B (SOIL) IC
0
5
10
15
20
25
30
35
40
45
50
1AL 1AU 2AL 2AU 3AL 3AU 4AL 4AU 5AL 5AU 6AL 6AU 7AL 7AU 8AL 8AU 9AL 9AU 10AL 10AU 11AL 11AU 12AL 12AU
Pea
k H
eigh
t µ
S
Sample
Ion composition lower vs upper soil fraction
Unk5
Unk4
Unk3
Unk2
Sulphate
Phosphate
Nitrate
Bromide
Nitrite
Unk1
Chloride
Bromate?
Formate?
Acetate
Fluoride
Grave
Control
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CASEWORK SAMPLES B (SOIL) GC, 28-Aug-2015 + 07:10:36
2.59 4.59 6.59 8.59 10.59 12.59 14.59 16.59 18.59 20.59 22.59 24.59 26.59 28.59 30.59 32.59 34.59Time0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
Positive control ext 270815 Scan EI+ 208+249+263
1.67e816.91
7.65
19.86
Negative control ext 270815 Scan EI+ 208+249+263
4.57e64.37
3.47
9.177.67
6.10 8.5111.119.85 13.7212.72 18.06
22.46 25.8224.35
Sample 1 AU 270815 Scan EI+ 208+249+263
5.63e716.92
4.40 9.107.68 22.40
Sample 4 AU 270815 Scan EI+ 208+249+263
4.32e64.37
3.823.06
9.07
7.676.115.3211.129.85 12.72 18.0613.72 19.52 26.9825.8520.89 22.95 28.87 31.53
Sample 6 AU 270815 Scan EI+ 208+249+263
4.70e64.40
3.67
9.127.68
6.135.34
11.119.85 12.71 18.0613.71
16.92 19.52 26.44
Sample 8 AU 270815 Scan EI+ 208+249+263
4.40e64.39
3.86
9.15
8.526.115.33
7.6711.11
9.85 18.0516.9212.72 13.71 19.51 22.75 24.72 33.0128.11
Sample 12 AU 270815 Scan EI+ 208+249+263
3.60e64.38
3.833.09
9.07
7.686.12
5.33
8.52 11.129.85 18.0612.72 13.72 19.52 27.3020.51 24.9222.30 23.36 26.05 29.6928.69 31.78
, 28-Aug-2015 + 07:10:36
2.59 4.59 6.59 8.59 10.59 12.59 14.59 16.59 18.59 20.59 22.59 24.59 26.59 28.59 30.59 32.59 34.59Time0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
Positive control ext 270815 Scan EI+ 208+249+263
1.67e816.91
7.65
19.86
Negative control ext 270815 Scan EI+ 208+249+263
4.57e64.37
3.47
9.177.67
6.10 8.5111.119.85 13.7212.72 18.06
22.46 25.8224.35
Sample 1 AU 270815 Scan EI+ 208+249+263
5.63e716.92
4.40 9.107.68 22.40
Sample 4 AU 270815 Scan EI+ 208+249+263
4.32e64.37
3.823.06
9.07
7.676.115.3211.129.85 12.72 18.0613.72 19.52 26.9825.8520.89 22.95 28.87 31.53
Sample 6 AU 270815 Scan EI+ 208+249+263
4.70e64.40
3.67
9.127.68
6.135.34
11.119.85 12.71 18.0613.71
16.92 19.52 26.44
Sample 8 AU 270815 Scan EI+ 208+249+263
4.40e64.39
3.86
9.15
8.526.115.33
7.6711.11
9.85 18.0516.9212.72 13.71 19.51 22.75 24.72 33.0128.11
Sample 12 AU 270815 Scan EI+ 208+249+263
3.60e64.38
3.833.09
9.07
7.686.12
5.33
8.52 11.129.85 18.0612.72 13.72 19.52 27.3020.51 24.9222.30 23.36 26.05 29.6928.69 31.78
, 28-Aug-2015 + 00:09:06
14.71 15.71 16.71 17.71 18.71 19.71 20.71 21.71 22.71 23.71 24.71Time0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
Sample 1 AU 270815 Scan EI+ TIC
6.09e822.40
18.06
16.9216.19
15.55 16.66
17.84
17.67
19.3318.80
18.25
19.85
20.4021.04
21.78 22.67 23.75
Sample 4 AU 270815 Scan EI+ TIC
4.30e818.06
17.8316.19
15.5416.65
17.58
18.63
20.4019.8419.53 21.04 21.78 22.66
Sample 6 AU 270815 Scan EI+ TIC
6.25e818.05
17.8316.19
15.54 16.65 19.3618.80 19.84 20.40 21.03 21.77
Sample 8 AU 270815 Scan EI+ TIC
5.50e818.05
17.8316.18
15.5416.65 19.3518.63 19.84 20.39 21.02 21.77
Sample 12 AU 270815 Scan EI+ TIC
4.72e818.06
17.84
16.19
15.5416.65 19.36
18.80 19.84 20.40 21.03 21.78
ChemicalSpiked (μg·L-1)
Found (μg·L-1)
Recovery %
Methylamine 15.5 1.7 10.72
Putrescine 44.1 4.0 9.17
Cadaverine 51.1 3.7 7.32
LocationPutrescine
(μg·L-1)Cadaverine
(μg·L-1)
1 AU (Grave) 149.2 < LOD
6 AU (Downhill) < LOQ < LOD
8 AU (Uphill) < LOQ < LOD
12 AU (Control) < LOD < LOD
Recovery
Quantification
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LABRADOR (Lightweight Analyzer
for Buried Remains and Decomposition Odor Recognition)
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Caylee Marie Anthony
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“Jose Baez harassing Dr. Vass about the handheld "sniffer machine" nicknamed "Labrador," designed to detect human
decomposition by reading such air samples. “
http://www.acandyrose.com/caylee_anthony_murder_trial_060611.htm
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Confounding variables& getting the research into the Criminal Justice System
Casey Anthony Trial - Dr. Vass and the hamburger theory
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Confounding variables& getting the research into the Criminal Justice System
Trial - Dr Vass tells Baez You can't take it out of context
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That Courtroom look………
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UK Taphonomy facility (?)
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Where to start ?
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The future – a simple chemistry set kit?• SIRCHIE® Fingerprint
Laboratories introduces the NARK® II Progressive System for Drug Identification. NARK® II has the capability of presumptively identifying several families of substances suspected of being abused drugs. Designed to function as a transportable narcotics laboratory, it is available for use wherever the need for its capability might arise.
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The way forward…..next steps
• Continue to investigate and develop thechemistry methodology
• Continue to work within the Burial ResearchConsortium
• Work more closely with Police colleagues todevelop and test the research further “in thefield”
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Thank you for listening