Multifactorial Traits
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Transcript of Multifactorial Traits
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Multifactorial TraitsChapter Seven
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Multifactorial• Not all diseases are Mendelian• Multifactorial = many factorsIn Genetics:• Multifactorial = both environment and
genetics (usually more than one gene)• Polygenic = more than one gene• Each gene separately follows Mendel’s
laws, but the trait overall does not
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Complex Traits• Disorder that is proven heritable, yet has
no clear mode of inheritance– Doesn’t follow Mendel’s laws
• More than one gene - polygenic• Interaction between genes - epistasis• Interaction between genes and
environment - multifactorial
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Why Common Complex Disorders and Rare Mendelian Disorders?
• Evolution can act upon a single detrimental gene – negative selection
• Gene functions that are good for some things, but can be harmful in excess rational fear vs. anxiety disorders
• Normal alleles only predisposing – other mutations/environment present
ex
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Genes vs. EnvironmentP
erce
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enet
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ontro
l 100
50
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Eye Color Car AccidentHeart Disease
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Genes vs. EnvironmentP
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nviro
nmen
tal C
ontro
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100
50
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Eye Color Heart Disease Car Accident
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Causation• There are only two factors that cause
any trait:1. Genetics2. Environment
• Nature vs. Nurture
• Interaction of two is multifactorial
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Multifactorial Traits:• Height• Weight• Eye color
– although basically no environmental component is involved
• Skin color• Heart Disease• What else?
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Quantitative Traits• Quantitative = measurable traits• Disease state is Qualitative
– Affected vs. unaffected– Show trait vs. do not show trait Autistic or normal?
• Multifactorial traits are often Quantitative– Height ? – quantify in inches– Weight ? – quantify in pounds “speaking ability is stuck at 2 years of age”ex
ex
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Quantitative Traits• Quantitative traits produce a continuum of
phenotypes:
Height (in)
# In
divi
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Quantitative Traits• Disease state may be beyond “threshold”
Speaking Ability
# In
divi
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Autistic
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Quantitative TraitsRather than genes people often talk about:• Quantitative Trait Loci (QTL) =
chromosomal regions that have been associated with a complex trait
• If a QTL is correct then one of the genes residing in this region should be directly involved in causing trait
Remember – More than one gene!therefore – more than one QTL too
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How to identify QTL
1. Linkage
2. Animal Models
3. Association Studies
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QTL Mapping• Start with a complex trait of interest• Phenotype a large group of individuals for
trait – quantitatively • Genotype everyone
• Do people who share the trait also share specific genomic regions (QTL) more often than chance?
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Wait a Second!?!• What if the trait we are interested in is
NOT genetic at all?• What would happen then?
• No matter how hard you studied, you would never find the genes, because there are none!
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1st: Is Trait Genetic?• Calculate Heritability
• Heritability (H2) = proportion of the trait that is controlled by genetics– H2 = 100 %– trait is fully genetic– H2 = 0 % – trait is fully environmental
• Complex traits are somewhere in between
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Heritability• Different ways to calculate/estimate• All based on this:
# of relatives that share the trait# expected to share if trait was 100% genetic
• Expected is calculated based on amount of genetic material shared between two relatives
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Heritability – Expected:• Use “Correlation Coefficient”• Fancy way of saying – how related are
these two people?
Relationship Percent Genes SharedMonozygotic Twins 100%
Dizygotic Twins 50%Siblings 50%
Parent and Child 50%Aunt or Uncle 25%Grandparent 25%First Cousin 12.5%
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Heritability• Gather a group of relatives
– 100 sibling pairs– 100 MZ twins
• Phenotype for trait: 40 of 100 sib pairs share trait– .40/.50 = 80 %– Therefore, trait is 80 % controlled by genetics– 20 % controlled by environment
ex
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Problems with Heritability?What do you think is wrong, or lacking, with
H2 estimate from relative pairs?
1. Families share genetics AND environment
2. More shared genetics usually correlates to more shared environment too:
Twins usually share bedroom Siblings raised in same house, unlike cousinsex
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Separate Genes from Environment
1. Adopted children • Compare birth parents to adopted parents• Sharing environment only, not genes
2. Twins• Compare MZ vs. DZ twins• Differences in twins is only difference
between amount of genes shared, environment is constant
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Concordance Rates• Compare the percent of MZ twins that are
concordant for the trait vs. the percent of DZ twins concordant
• If MZ twins are more concordant than difference is attributed to genetics Autism: 90% MZ vs. 2% DZ Homosexuality: 52% MZ vs. 20% DZDepression: 46% MZ vs. 9% DZ
ex
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Problem with Concordance?More shared genetics usually correlates to
more shared environment too– MZ twins can be raised in more similar
environments than DZ twins
Tuberculosis was found more often in MZ twins than DZ twins
– Obviously TB (caused by bacteria), is not genetic at all!
ex
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What would be ideal?
• MZ twins, separated at birth, raised completely apart
• Animal models, where one can separate the genetics from the environment
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Genetic Counselors• What happens when parents ask you the
risk of having a child with a complex disorder?
• Cannot calculate an exact statistical likelihood based on Mendel’s laws
Instead give:• Heritability estimates• Empiric Risk
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Empiric Risk• The incidence of the trait in a specific
population:Differs based on ethnic background:
Also depending on whether relative has trait:
Ethnic Background Prevalence of DiseaseCaucasian 50
African 3.8African-American 26Native American 5.9
Relationship to Affected Individual Risk of RecurrenceIdentical Twin 40%
Sibling 5%Child 3.5%
First Cousin 0.30%
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QTL Mapping• Start with a complex trait of interest
– That shows strong genetic contribution• Phenotype a large group of individuals for
trait – quantitatively • Genotype everyone
• Do people who share the trait also share specific genomic regions (QTL) more often than chance?
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QTL Mapping1. Linkage:
- Marker and phenotype are correlated
2. Animal Models- Animal can be manipulated to show
phenotype- Compare differences in genetics, matched to
differences in phenotypes
3. Association Studies:- Are certain alleles correlated with phenotype?
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Association Studies
“Linkage Disequilibrium” – alleles are inherited together (rather than genes)– LD only ranges a short distance – ~ 10,000 bases– Because alleles are so close they are always
inherited together (no crossing over)• Association comparing alleles• Linkage usually done in families,
association usually done case vs. control
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Case vs. Control• Gather a group of individuals with trait• Compare them to a group of individuals
who do not have trait• Whatever alleles (genes) are different =
cause of trait• Problems?
– What about ethnic genetic background?– Need to perfectly match the background of
the two groups
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Case vs. Control
• Perfect match = family membersAssociation studies are now often done in:– Sib pairs (two siblings, both affected)– Trios (two parents and one affected child)
• 2nd best = genotype both groups across entire genome– Make sure genetic backgrounds are the same– “Genomic Controls”
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Complexity of Trait
• More complex the trait, more difficult it will be to identify QTL or genes involved
# In
divi
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eede
d
100
50
1000
# Genes Causing Trait1 2 3 4 5
This is assumingenvironment is
completelycontrolled for
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Summary1. Figure out whether trait is genetic:
• Compare relatives vs. general population• MZ vs. DZ twins• Adoption Studies
2. Estimate how genetic:• Heritability• Empiric Risk
3. Try to identify genes involved in trait(QTL’s)
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SummaryQTL = regions associated with phenotype:
– Linkage – correlation between genomic regions and phenotype
– Association – correlation between alleles and phenotype
– Animal Models• Association Studies can be:
– Case vs. Control– Sib pairs or Trios
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Important Terms:Empiric Risk – known incidence of trait in
particular populationHeritability – estimate of percentage of trait
that is caused by geneticsCorrelation Coefficient – proportion of genes
two relatives shareConcordance – Percentage of twin pairs that
both show phenotype
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Example: Obesity• Qualitative = obese, overweight, normal• Quantitative = weight in pounds• Multifactorial
– Controlled by environment– Controlled by genetics:
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Next Class:• Read Chapter Eight
• Homework – Chapter Seven Problems;– Review: 1,4, 7,8,10– Applied: 1,3,5,6,10,11