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Lo spettrometro NIR MPA II: potenzialità applicative in campo agroalimentare

MPA II. NIR IN A NEW LIGHT.

Innovation with Integrity

Bruker Corporation Groups and Divisions(NASDAQ: BRKR)

Bruker BioSpin Group

Innovation with Integrity

Magnetic Resonance Spectroscopy (MRS)

Preclinical Imaging (PCI)

Bruker CALID Group

Mass spectrometry (Daltonics)

Infrared & Raman spectroscopy (Optics)

Bruker NANO Group

Bruker Nano Surface

Bruker EST (BEST)

Supercon wire and devices

Applied, Industrial and Clinical (AIC)

Bruker Nano Analytics

X-ray analysis

CBRNE (Detection)CALID = Chemical, Applied Markets, Life Science, In-Vitro Diagnostics, Detection

BEST: Bruker Energy and Supercon Technologies

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Bruker Optics – Innovation Leader

FT-NIR Spectrometer

Research & Development Spectrometer

IFS

11

3v,

mod

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vac

uum

rese

arch

FT-

IR

IFS

11

2se

ries

, Bru

ker

Opt

ics

first

FT-

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pect

rom

eter

IFS 85, first FT-IR for Routine Industry Applications

IRscope I, first FT-IR Microscope

VECTOR/N MATRIX MPA

IFS 1

20IF

S 8

8

IFS 28/N

FT-IR Routine Spectrometer

TENSORVECTORIFS 45 IFS 25

FT-IR Microscopes

IRscope II HYPERION

FT-Raman SpectrometerFRA 106 RFS 100

1998

Sentinel

Dispersive Raman

Senterra

ConfocheckIFS 28/BLife Science Systems

IFS 2

8

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Bru

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-IR S

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1974

1976

1981

1984

1985

1986

1988

2004

2002

2000

1990

1992

1994

1996

2006

2008

2011

2012

LUMOS

TANGO

ALPHA

Mobile-IR

2015

2016

Senterra II

MPAII

2018

INVENIO

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Bruker Optics - Market Segments

CPPChemical, Pharma & Polymer

AFFAgri, Food & Feed

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NIR: principio di base della tecnica

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Principi della spettroscopia NIR

0 1 2 3 4 5 6 7Distanzadilegame

0

2

4

6

8

10

12

14

elaiznetoPid

esroM

v=0v=1

v=2v=3

-+

d B

A

0x

xµ∂ ≠ ∂

• Nella spettroscopia (N)IR si fornisce al sistema l’energia per passare dallo stato fondamentale a primo stato eccitato

• Non tutte le possibili vibrazioni sono in grado di interagire con le radiazioni elettromagnetiche

• regole di selezione

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NIR: assorbimenti caratteristici

i legami chimici che assorbono (principalmente) in questa regione sono:• OH• CH• NH

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NIR: Tecnologie disponibili

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Perché FT?

• Eccellente rapporto segnale/rumore: tutte le lunghezze d’onda vengono raccolte simultaneamente vantaggio di Fellgett.

• Maggiore efficienza ottica Vantaggio di Jacquinot.• Elevata accuratezza della misura della lunghezza d’onda: è

garantita dall’utilizza di un riferimento interno (laser) che emette ad una lunghezza d’onda nota e stabile Vantaggio di Connes.

• Elevata risoluzione ottica: permette di ottenere spettri ad alta risoluzione, utili nel NIR non tanto per scopi applicativi quanto per permettere la verifica dell’accuratezza degli strumenti Standardizzazione degli strumenti

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La trasformata di Fourier

OPD

Segn

ale

Det

ecto

rl

5001,0001,5002,0002,5003,0003,5004,000Numero d’onda, cm-1

0.10

0.20

0.30

0.40

Inte

nsità

Sin

gle-

chan

nel

Interferogramma SpettroFTIFT

2( ) ( ) i xS I x e dxπνν∞

−∞

= ∫

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Interferometro

Sorgente

Detector

Specchiomobile M2

Specchiofisso M1

Beamsplitterx=0

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ROCKSOLID Interferometer

Plane Mirrors in a classical Michelson Interferometer

Cube-Corner Mirrors in a ROCKSOLID-Interferometer

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ROCKSOLID Interferometer

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Bruker’s wear-free cube corner interferometer is state-of-the-art for modern near infrared spectrometers: Non-sensitive to vibrations Non-sensitive to temperature

fluctuations Highest repeatability: 0.006 cm-1

Excellent accuracy: 0.1cm-1

Highest resolution: 2 cm-1

…and permanently aligned!

ROCKSOLID Interferometer

Tecniche di campionamento

25/05/2018 17

Campione

Rivelatore

Sorgente

Trasmissione

Campione

RivelatoreSorgente

Riflettanza diffusa

Campione

RivelatoreSorgente

Specchio

Transflettanza

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Each customer gets exactly the instrument that matches the application. There is no need to purchase anything that is not wanted or needed.

MPAII: Tailor-made Production

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Maximum Flexibility

Sample Compartment

IntegratingSphere

Fiber OpticProbes

ExternalTransmission

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Configurazione strumentale disponibile

MPA II MPA II basic, with integrated heatable and movable sample compartment for liquidsMPA II-R Extension module MPA-R (integrating sphere)IN312/C Rotating sample cup holder, 97mm w/o sample cupIN312-B Adaption for Petri dishesIN312-P Sample cup for IN312 (ABS)IN311-O/C Rotating sample cup holder, 51 mm w/o sample cupIN311-P Sample cup for IN311, 51mm diameterO/NIR_8.0+ OPUS/IR ver. 8.0O/QU-N OPUS Software "Quant"IN481S-2 Accessory for transflection measurements of liquidsIN441-001+ Glass vials, plain top, with flat bottom, 186 pieces w/o capsIN601CM Reduction insert for sample compartment for polymer cuvettes (IN455)IN601CRA Insert for cuvette-holder 10mmIN415QX-01 Rectangular flow cell (HELLMA 170-QX)

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• Customizable workspaces• Easy measurement option• Setup of the instrument with

a few mouse clicks• Display informs user about

instrument status• Chemometrics quant

modeling (PLS) with self optimization function

Software OPUS: Easy Operation

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• The MPA II comes with a new generation of light sources with a MTTF of 25,000 hours:

More peace of mind for the daily routine operation

Longer service intervals lead to reduced maintenance cost

Long-life NIR Light Source

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• All detectors inside the MPA II are temperature-controlled and work linearly over the whole spectral range.

• This is essential for a successful quantitative analysis:

Highest accuracy and reliability

Highest reproducibility

High-sensitivity InGaAs detectors

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• New durable diode laser for:

Highest wavenumber accuracy

Extra long lifetime (MTTF > 10 years)

Solid state laser technology

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• easy sample insertion, no drawer or lid to open

• Moving insert for automated background measurements

• Temperature control as standard• A wide choice of vials, cuvettes

and flow cells available

Sample Compartment

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• Ideal for the measurement of heterogeneous samples

• Wide range of accessories like sample rotators and vial holders available

• 30-position sample wheel for automated measurements

• Fully compatible to the compact TANGO-R and Matrix-I analyzers

Integrating Sphere

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Integrating Sphere

Ideal for powders, granules or heterogeneous samples

Detector

Gold-coated integrating sphere

NIR Beam

Sample

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• Small Rotator: Ø 50 mm• Large Rotator: Ø 97 mm• XXL Rotator: Ø 370 mm

Accessories: Rotators, Cups and Petri dishes

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The MPA II is equipped with an integrated multiplexer so that two different probes can be attached:• investigation of solids and liquids

with only one instrument • no unplugging and changing of

the probes• quick and easy handling –

reproducible, reliable, and robust

Fiber Optic Probes

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LSM Module

Liquid Samplig Module

• Fully automated liquid analysis and cleaning

• Homogenizer for raw milk

• Bypass pump for other liquid samples, e.g. cream and whey

• Handles high viscosity liquids, e.g. milk drinks, whey protein concentrates and permeates

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LSM Module

Liquid Samplig Module

• 1 mm quartz Flowcell, easy to clean

• Calibrations can be easilytransferred to in-line applications

• Automatic routine for Homogenizer valve efficiency test

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Performance example

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Milk and Dairy Products with MPAII-D

MPA II: The tailor-made Solution for your Quality Control

http://www.niritalia2018.sisnir.org/

http://www.nir2019.com/

Innovation with Integrity