MOVING LIQUIDS with SOUND - Jefferies Group Inc.p… · 2 Liquid Handling Billions have been...
Transcript of MOVING LIQUIDS with SOUND - Jefferies Group Inc.p… · 2 Liquid Handling Billions have been...
© 2016 LABCYTE INC. All rights reserved. Labcyte®, Echo, the Labcyte logo are registered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.
MOVING LIQUIDS with SOUNDLabcyte Overview
Mark Fischer-Colbrie
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Liquid Handling
▸ Billions have been invested in developing advanced analytical and detection tools
▸ Antiquated liquid handling has reached its physical limits and is a bottlenecj
▸ Urgent need for improved liquid transfer
▸ Precision Medicine needs precise liquid handling
▸ Achieving $1000 Genome (and going to $100 Genome) requires miniaturization of expensive
reagents
▸ Biology is complex – complexity requires more testing and lower cost per data point
Sample Collection
Advances in Analysis Have OutpacedSample Preparation
Healthcare Research and Diagnostic Workflow
Sample PrepDetection and
Analysis
MOVING LIQUIDS with SOUND | Labcyte Overview
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Positioned to disrupt existing markets / expand current markets
Acoustic Dispensing Addresses ~$3B Market
Robotics
Automated
Liquid
Handling
$640M $140M
Manual
Liquid
Handling
Echo
Benchtop
$600M
High / Mid
Throughput
Readers
Echo-MS
$600M
Genomics
OEM /
New
Configurations
$540M
Biobanking /
Tubes
Acoustic
Tubes
$165M
Disrupting
Access
Workstation
Echo Liquid
Hander
Enabling
$780M ~$2B
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Precision liquid handling -- for the first time
Labcyte Overview
▸ Established 2000 - Invented touchless liquid handling using focused sound waves.
- Digital acoustic dispensing
- First Echo® Liquid Handler Shipments 2004
- Set up to be a global company to initially support global pharmaceutical firms
▸ Hundreds of Customers- 20 of top 20 pharmaceutical companies
- Large and mid-size pharma, biotech, molecular diagnostics, genome centers,
synthetic biology, precision medicine centers
- Academic & research institutes
- Contract research organizations
▸ Hundreds of Applications- Drug Discovery – Precision Medicine
- Genomics – Molecular Diagnostics
- Proteomics – Mass Spectrometry Interface
▸ Patents- 58 Issued US patents / multiple global patents from in-house inventions
- Additional patent applications filedMOVING LIQUIDS with SOUND | Labcyte Overview
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Hundreds of Customers Worldwide
MOVING LIQUIDS with SOUND | Labcyte Overview
Numerous Prestigious Industry Awards
Labcyte has received a number of awards from organizations like The R&D 100, The World Economic Forum, Ernst & Young, Frost & Sullivan, among others, who are recognizing the Company’s impact
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Top 100 Global Companies (2013)
Select Industry Awards
2007
Top 3 Life Science
Company 2013
World Economic Forum
2015
Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
Problems with Traditional Systems
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Traditional Liquid Handling Issues
Human
Errors
55% 50% 44% 36% 28% 19% 8% 6%
Liquids Stick
to Tip
Viscosity
Reduces
Accuracy
Immersed
Tip Carry
Over
Liquid
Surface
Tension
Reduces
Accuracy
Pipettes
Take Up
More
Liquid
Liquids
Evaporate
Liquid
Temperature
Labcyte Acoustic Droplet Ejection
Eliminates the Top 6 Sources of Errors
Top Sources of Pipette Errors
MOVING LIQUIDS with SOUND | Labcyte Overview
Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
A Sound Solution
Enter Title of Presentation | Enter Date
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Echo Liquid Handler in Action
Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
The Labcyte System
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Innovative system centered around the transfer of precise amounts of fluids without contact
Groundbreaking System Solution
Labcyte® Automation
Incorporates Echo Liquid
Handlers for complete
workflow solution
Enables Labcyte to own the
sales process
Labcyte® Consumables
Proprietary, acoustically
qualified source plates
Precise manufacturing and
qualification process
Echo® Software
Applications
Array of applications that
maximize the utility and
flexibility of Echo Liquid
Handlers
Echo® Liquid Handler
Revolutionizes liquid handling
by using acoustic energy to
transfer liquids
MOVING LIQUIDS with SOUND | Labcyte Overview
Direct
Distribution
Both
Global Commercial Organization
Labcyte has been built with the vision to support global pharma from day one
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• 52-person global field team -- sales, field applications, service and support
• Fully integrated field support team with a single CRM for all activities
North America Field Team
Asia / Australia Field Team
Europe Field Team
Source: Company Management.
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Labcyte has experienced extraordinary momentum from industry-leading science publications. As of 2016,
the Echo acoustic liquid handler has been featured in over 600 cumulative publications.
Extraordinary Momentum Across Applications
4778
106
151
211
281
376
466
635
-
100
200
300
400
500
600
7001 Source: Company Management. Represents
calendar year ending December 31, 2016.
Cumulative Publications1 Journal of Laboratory Automation Special Issue on ADE
▸ Highlights the impact of acoustic
dispensing throughout research
▸ Key topics include:
• Personalized Medicine
• Synthetic Biology
• Mass Spectrometry Interface
• Combination Screening
• Antibody Screening
• Genotyping
• Impact on High-throughput Screening
▸ 23 papers total
Available at www.labcyte.com/jala
MOVING LIQUIDS with SOUND | Labcyte Overview
Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
Market / Application Spotlight
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Used in life sciences, research, drug discovery and personalized medicine
Broad Range of Applications
Genomics Drug Discovery
Synthetic
Biology
Increase
efficiency
and speed
while
reducing
costs
Low cost,
highly
efficient
library
preparation
Enabling
high-
throughput
epigenetic
screening
Cost-
effective,
high-
throughput
RT-qPCR
From
personalized
medicine
towards
precision
medicine
Discover the
right drugs
with
improved
transfer
performance
Biologically
relevant
assays with
unmatched
data quality
Simplify
assay
workflows
with precise
reagent
transfers
Enable cost-
effective,
earlier safety
screening
Increase
efficiency
and speed
while
reducing
costs
Sequencing Epigenetics
Gene
Expression
Precision
Medicine
Translational
HTS /
Secondary
Screening
Cell-based
Assays
Biochemical
Assays ADME-Tox
Combination
Screening
▸ Drug Discovery: Enabling miniaturization with unparalleled
throughput and accuracy
- Result: New drugs discovered
▸ Genomic Research: Overcoming traditional barriers by
dramatically reducing sample and reagent volume requirements
- Result: Better experimental data at a fraction of the cost
▸ Cancer Research: Helping research programs drive down assay costs
while enabling new experimental workflows
- Result: Patient sample drives therapy
▸ Molecular Diagnostics: Eliminating cross-contamination and other data
errors while dramatically reducing running costs
- Result: Lower operational costs (critical with reimbursement pressures)
MOVING LIQUIDS with SOUND | Labcyte Overview
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Finds Drugs Missed by Traditional Systems
TABLE A
Compound # Echo IC50 (µM) Tecan Genesis IC50
(µM)
4 0.003 0.146
5 0.002 0.553
6 0.007 0.973
7 0.003 0.778
8 0.004 0.445
9 0.052 0.170
10 0.064 0.817
11 0.486 3.03
▸ Therapeutics would have been missed without the Echo
▸ AstraZeneca patent #7,718,653 presents data showing
compounds ~10x – 300x more potent using acoustics
▸ Pipette tips inhibited accurate drug effectivity analysis
AstraZeneca discovers cancer drugs with the Echo that would
have been missed using traditional technologies
MOVING LIQUIDS with SOUND | Labcyte Overview
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Tips give the wrong answer….
MedImmune study finds touchless acoustic transfer insures quality results for peptides
B
B
AThe EC50
value
changes
when the
diluents are
changed
when using a
pipette
The EC50
value is
constant
when the
Echo
Liquid
Handler is
used
Tip-based System Echo System
A changing
concentratio
n of BSA in
the solution
changes the
apparent
EC50 when
using a
pipette
No such
shift is
seen with
the Echo
system
A BRAVOBSA Curve Shift in rGLP-1R
Echo SystemBSA Curve Shift in rGLP-1R
MOVING LIQUIDS with SOUND | Labcyte Overview
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Comparison of Traditional vs. Echo
Echo® Liquid Handling for Genomics | Labcyte Introduction
▸ Volumes
• Traditional: 20 µL
• Echo: 5 µL
▸ Reaction assembly
• gDNA on Traditional: 8 µM
• gDNA on Echo: 5 µM
• Primers: 625 nM
• Master Mix: 1x Type-It Fast
SNP PCR (Qiagen)
© 2016 LABCYTE INC. All rights reserved. Labcyte®, Echo®, and the Labcyte logo are registered trademarks or trademarks of Labcyte Inc.
Genomics Product PositioningNext-generation Sequencing
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Miniaturization in Genomics
Echo® Liquid Handling for Genomics | Labcyte Introduction
Application Cost per Reaction(at recommended)
Miniaturized
Cost per ReactionVolume Reduction
(up to…)
PCR / qPCR $0.27 $0.05 6X
NGS Library Prep $72.91 $0.73 100X
Sanger Sequencing $0.26 $0.06 5X
MDA Setup $17.64 $4.41 4X
Gibson/Golden Gate DNA Assembly $15.90 $0.16 100X
Use fewer tips
Generate less waste
Reduce cross-contamination
Eliminate carryover errors
Increase throughput
Increase pool density / multiplex
Key Benefits
• Synthetic Biology
• Cancer Research / Personalized Medicine
• Drug Discovery
• Academic Research
• Diagnostics
Areas Impacted
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Enabled Personalized Medicine
• Global leader in personalized medicine
• Real-time patient data decision making for
treatment
• Repurposing cancer drugs with pharma
• Individualized system medicine - seminal
advancement in cancer therapy
• Connecting patient testing at the cell level
with genomic profiling
• Result
• Rescue extremely high risk patients
• Comprehensive data sets driving drug
development and repurposing
• Pfizer renal cancer drug being re-purposed
• Only possible using acoustic liquid
handling
• Initial work in blood cancers now being
expanded into solid tumors
Miniaturization and flexible combinations enable new applications:
Institute of Molecular Medicine Finland (FIMM)
“This research opportunity is changing the way new drugs are introduced for patients: It is truly-game changing. Labcyte technology is a key platform for this next stage.”
Olli Kallioniemi, FIMM Director
MOVING LIQUIDS with SOUND | Labcyte Overview
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Multiple findings in AML and CML treatment published in leading publications
Enabled Repurposing of Cancer Drugs
May 1, 2015
• Novel drug candidates for advanced phase and blast crisis CML
• Three classes of drugs highly sensitive for most TKI resistant patient samples
February 1, 2015
• Joint development between FIMM and Pfizer
• Axitinib as a potential inhibitor to drug resistance
• Studied cancer cells from patients with CML and AML
• Study relied on FIMM’s Drug Sensitivity and Resistance Testing (DSRT) methods
• Lead the way for proof-of-concept clinical studies
• The market availability of axitinib, (trade name Inlyta) means testing clinical utility for leukemia can be fast-tracked
September 20, 2013
• Identified potential new pathway for targeting AML treatment
• Conducted one or more combination screening of drugs using patient cells
• Had an impact on all patients treated
• Identified 3 drugs that may be considered for re-purposing for AML purposes
MOVING LIQUIDS with SOUND | Labcyte Overview
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Enabled High-throughput Synthetic Biology
• Innovator in microbial engineering and
screening technologies
• Renewable products: fuels, cosmetics,
biopharmaceuticals
• Recent publication demonstrating significant
NGS prep cost savings
• American Chemical Society, April 25, 2015
• Illumina Nextera reagents were reduced 100-fold
($72 to 72 cents)
• Reduced time from 18 hours to 3 hours
Major improvements in cost and processing efficiency for
microbial engineering
"By combining an Illumina MiSeq platform with a Labcyte Echo acoustic liquid dispensing system, we have developed a rigorous, low-cost QC method…”
Elaine Shapland, Scientist
MOVING LIQUIDS with SOUND | Labcyte Overview
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Case Study:
• Progressive adoption of Echo Liquid Handler
and Access workstation
• Compound management
• Protein Crystallography
• Cell based assays (HTRF)
• GMP
• Mouse tail genotyping
Numerous expansion opportunities within
existing customer base will continue to drive
organic growth
“These instruments are phenomenal when comparing them to traditional liquid handling robots - they are much faster, much more accurate and precise, and highly reproducible. The company is a pleasure to interact with and is structured in a collaborative manner that easily facilitates research and partnership projects.”
Colin Cox, Science Manager
8 traditional systems replaced for one application
MOVING LIQUIDS with SOUND | Labcyte Overview
Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
Research and Development
(announced)
Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
Echo-MS
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▸ High-throughput, low cost, label-free analysis
Echo-MS: Acoustic Mass Spec Interface
▸ Large pent up demand for improved sample prep and delivery systems for mass
spectrometry
▸ Biopharma, government and academic accounts have limited adoption of MS
based detection for high throughput needs due to performance and cost barriers
▸ Key benefits of MS detection for these customers include:
– Label free detection with multiple end point measurements
– Wide range of end points (Proteins, lipids, sugars, DNA)
– True high content read out enable pathway analysis and metabolomics
MOVING LIQUIDS with SOUND | Labcyte Overview
• Large reagent cost savings on a 1 million compound screen are extremely large.
• Traditional cost: $150,000
• Acoustic MS Cost: $1500
• 10 times faster than fastest options
• Assay developed in 4 days as compared to usual time of 4 months
• Kinetic data acquisition enables assay optimization in 4 days instead of 4 months
• Major game changer in design of experiment
• False positives eliminated
• Z’ values at .70
• All active hits were confirmed with LCMS
Echo-MS SuccessesPresented by AstraZeneca at SLAS and later ASMS
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Echo® Acoustic
LIQUID HANDLING
The Future of Science is Sound
MOVING LIQUIDS with SOUND | Labcyte Overview
Acoustic Tubes and Instrumentation
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Acoustic Tubes: Product Overview
• Funding by AstraZeneca
• Partnership with Brooks
• New, 96 rackable acoustic storage tube as new recurring
revenue source for Labcyte
• Development of updated Echo 555 platform for tube and
plate dispensing
• AstraZeneca is completely revamping their tube storage
system to take advantage of acoustic transfer
Labcyte’s acoustic tube and instruments program delivers a solution to the unmet market need for fully
acoustic workflows for small molecule and biologic storage
Collaboration Overview
MOVING LIQUIDS with SOUND | Labcyte Overview
© 2016 LABCYTE INC. All rights reserved. Labcyte®, Echo, the Labcyte logo are registered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.
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