More Performance, Less Space - Bruker...In comparison, the new single story Ascend Aeon 900 MHz...
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Single-Story Ascend Aeon 900 MHz New superconducting wire technology, combined with active refrigeration, has allowed a substantial reduction in the size of actively shielded 900 Mhz magnets. The first customer installation at UCSD (Professor S. Opella) was for a dedicated solid-state spectrometer in 2013. This is now followed by the first solution-state 900 Mhz Ascend Aeon at the University of Bayreuth (Professor P. Rösch). More Performance, Less Space 900 MHz magnet evolution: US2 (ultra-stabillized & ultra-shield) 900 MHz magnet generation, introduced at ENC 2004 (left). In comparison, the new single story Ascend Aeon 900 MHz magnet available now (right). Conventionally sampled 3D BEST-TROSY HNCO of a 7 kDa protein. 0.3 mM concentration, ns = 8, 64 x 64 increments for 15 N & 13 C evolution, 1 hour 36 minutes total acquisition time. 1 H- 15 N projection (left), 1 H- 13 C projection (center) and indicated 1 H traces of 3D (right) are shown. Data courtesy of Prof. P. Rösch and Dr. K Schweimer, University of Bayreuth. 25% sparse sampled 3D 15 N-edited 1 H- 15 N- 1 H NOESY-HSQC of a 0.3 mM 7 kDa protein. 64 ( 15 N) x 384 ( 1 H) increments, ns = 8, 20 hours 30 minutes total acquisition time. 1 H- 1 H projection (left), 1 H- 15 N projection (center) and selected strips (right) are shown. Data courtesy of Prof. P. Rösch and Dr. K. Schweimer, University of Bayreuth. 1 H- 13 C Methyl-TROSY of a 0.38 mM concentrated 43 kDa ILV-labeled protein. 2k ( 1 H) x 1k ( 13 C) data matrix acquired with ns = 4. 1 hour 40 minutes total acquisition time. Data courtesy of Prof. P. Rösch and Dr. K. Schweimer, University of Bayreuth. 900 MHz Ascend Aeon at University of Bayreuth. Hα region of 1D 1 H spectrum of Cyclosporin 5.3 m min. ceiling height 3.66 m min. ceiling height Dispersion @ 400, 600, 900 MHz P. Schanda, H. v. Melckebeke & B. Brutscher, J. Am. Chem. Soc. 128, 9042-9043 (2006) E. Lescop, P. Schanda & B. Brutscher, J. Magn. Reson. 187 163-169 (2007) 3D BEST-TROSY-HNCO experiments 15 N edited NOESY-HSQC: 900 MHz Ascend Aeon 3D BEST-TROSY-HNCO 1 H [ppm] 03_ENC15_AA900.indd 1 4/16/2015 5:33:06 PM
Transcript of More Performance, Less Space - Bruker...In comparison, the new single story Ascend Aeon 900 MHz...
Single-Story Ascend Aeon 900 MHz
New superconducting wire technology, combined with active refrigeration, has allowed a substantial reduction in the size of actively shielded 900 Mhz magnets. The first customer installation at UCSD (Professor S. Opella) was for a dedicated solid-state spectrometer in 2013. This is now followed by the first solution-state 900 Mhz Ascend Aeon at the University of Bayreuth (Professor P. Rösch).
More Performance, Less Space
900 MHz magnet evolution: US2 (ultra-stabillized & ultra-shield) 900 MHz magnet generation, introduced at ENC 2004 (left). In comparison, the new single story Ascend Aeon 900 MHz magnet available now (right).
Conventionally sampled 3D BEST-TROSY HNCO of a 7 kDa protein. 0.3 mM concentration, ns = 8, 64 x 64 increments for 15N & 13C evolution, 1 hour 36 minutes total acquisition time. 1H-15N projection (left), 1H-13C projection (center) and indicated 1H traces of 3D (right) are shown. Data courtesy of Prof. P. Rösch and Dr. K Schweimer, University of Bayreuth.
25% sparse sampled 3D 15N-edited 1H-15N-1H NOESY-HSQC of a 0.3 mM 7 kDa protein. 64 (15N) x 384 (1H) increments, ns = 8, 20 hours 30 minutes total acquisition time. 1H-1H projection (left), 1H-15N projection (center) and selected strips (right) are shown. Data courtesy of Prof. P. Rösch and Dr. K. Schweimer, University of Bayreuth.
1H-13C Methyl-TROSY of a 0.38 mM concentrated 43 kDa ILV-labeled protein. 2k (1H) x 1k (13C) data matrix acquired with ns = 4. 1 hour 40 minutes total acquisition time. Data courtesy of Prof. P. Rösch and Dr. K. Schweimer, University of Bayreuth.
900 MHz Ascend Aeon at University of Bayreuth.
Hα region of 1D 1H spectrum of Cyclosporin
5.3
m m
in. c
eilin
g he
ight
3.66
m m
in. c
eilin
g he
ight
Dispersion @ 400, 600, 900 MHz
P. Schanda, H. v. Melckebeke & B. Brutscher, J. Am. Chem. Soc. 128, 9042-9043 (2006) E. Lescop, P. Schanda & B. Brutscher, J. Magn. Reson. 187 163-169 (2007) 3D BEST-TROSY-HNCO experiments
15N edited NOESY-HSQC: 900 MHz Ascend Aeon
3D BEST-TROSY-HNCO
1H [ppm]
03_ENC15_AA900.indd 1 4/16/2015 5:33:06 PM
