Monthly Report: Surveillance Project SB4008 IFNg tests for ...
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Monthly Report:Surveillance ProjectSB4008 IFNg tests forbovine tuberculosis
(TC0651 and TC0751)
Number
Report Period
Issued 10 February 2019
Operational notes on terminology, definitions, re-test and re-samples testoutcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which issubsequently submitted for testing. It is generally assumed that the number of samples andthe number of animals are analogous as any duplicate sample blood tube from the sameanimal will not be tested. However, minor discrepancies may occur where the same animal istested more than once due to a request for a resample or the animal is tested twice underdifferent categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.Submissions may comprise 1 or more samples (for example, if an entire herd is being tested itis usually sent as 1 submission). It is generally assumed that the number of submissions andthe number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on anew ELISA plate. Each sample can be retested only once. A retest is not a reportable testoutcome.
• Resamples are where a sample has been retested and failed a second time, so that the labrequests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: bloodcollected into wrong type of vacutainer, samples that have not been maintained at theappropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, bloodthat is extensively clotted (small clots are OK), samples received after 4pm on the day aftersample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure ofthe quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) mayindicate compromised blood quality as a result of collection/transportation conditions or duethe animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of thebackground antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)may indicate a laboratory procedures problem (normally resolved during re-test) or that ananimal has unusually high background levels of IFN-g.
Submission Reason Explanation Mandatory?
Possible Herd Slaughter(PAR-HERD-S)
Parallel interferon-gamma blood testing of skintest negative cattle to inform whole or partialherd slaughters decisions
YES, if APHA arecontemplating a herdslaughter
Persistent TB breakdowns(OTF status withdrawn)(PAR-P-CONF) N.B.PAR-PERSIS from 1st April2017
Parallel blood testing of skin test-negative cattlein persistently infected herds that have failed toresolve by repeated short-interval skin testingand fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence(PAR-LOW-IN) N.B.PAR-NEW-IN from 1st April2017
Parallel blood testing to maximise theprobability of removing all infected cattle in anew herd breakdown as soon as possible afterconfirmation of TB.
YES in Area of LowIncidence
Rapid Testing of twice IR's(PAR-RAPID)
Parallel blood testing of two-times IRs identifiedunder the severe interpretation of the skin testused in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise theprobability of removing all infected cattle in anew breakdown as soon as possible afterconfirmation of TB where herd is situated inbadger control areas of England that havecompleted at least 2 effective culls.
YES (in eligible badgercontrol areas of England)
PAR-CUL-P
Parallel blood testing to maximise theprobability of removing all infected cattle in apersistent confirmed breakdown where herd issituated within badger control areas of Englandthat have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other(PAR-OTHER)
Other parallel blood testing not covered in anyof the other scenarios NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skintest reactors and/or IRs in unconfirmed TBbreakdown herds to clarify their infection statuswhere there is evidence of non-specificsensitisation to bovine tuberculin (the“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud(SER-FRAUD)
Modified serial blood test of suspectedfraudulent reactors to the skin test (animals withabnormal skin swellings), in confirmed orunconfirmed TB incidents.
NO (APHA discretion tofirm up or rule out anysuspicion of fraud andsupport anyinvestigations)
Serial Other(SER-OTHER) Serial test – other reasons NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible testreadout in a confirmed M. bovis-infected herdwhere Johne's (M. a. paratuberculosis) infectionor vaccination is suspected to be interferringwith M. bovis infection detection
NO (APHA discretion)
Test Criteria
NumsamplesJanuary2019
% Total 2019 %
Submissions 289 289
Samples Total (%) 27513 100.00 % 27513 100.00 %
England 20585 74.82 % 20585 74.82 %
Wales 6928 25.18 % 6928 25.18 %
Scotland
Parallel Tests PAR-CUL-N 2848 10.35 % 2848 10.35 %
PAR-CUL-P 2527 9.18 % 2527 9.18 %
PAR-HERD-S 634 2.30 % 634 2.30 %
PAR-NEW-IN 13956 50.73 % 13956 50.73 %
PAR-OTHER 1605 5.83 % 1605 5.83 %
PAR-PERSIS 5313 19.31 % 5313 19.31 %
PAR-RAPID 173 0.63 % 173 0.63 %
Total(% of all samples) 27056 98.34 % 27056 98.34 %
Serial/Extended Tests SER-FLEXI 450 1.64 % 450 1.64 %
SER-FRAUD 0 0.00 %
SER-NSR 7 0.03 % 7 0.03 %
SER-OTHER 0 0.00 %
Total(% of all samples) 457 1.66 % 457 1.66 %
Total 27513 100.00 % 27513 100.00 %
Retests Total (% of all samples) 1937 7.04 % 1937 7.04 %
England 1526 5.55 % 1526 5.55 %
Wales 411 1.49 % 411 1.49 %
Scotland
Resamples Total (% of all samples) 1657 6.02 % 1657 6.02 %
England 1335 4.85 % 1335 4.85 %
Wales 322 1.17 % 322 1.17 %
Scotland
Rejects Total (% of all samples) 974 3.54 % 974 3.54 %
England 860 3.13 % 860 3.13 %
Wales 114 0.41 % 114 0.41 %
Scotland 0 0.00 %
Table 1. Monthly Totals for January 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother
PAR-CUL-N
PAR-CUL-PPAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPIDSER-FLEXI
SER-NSR
Breakdown ofReason forsubmission
PAR-CUL-N 2848 10.4 %
PAR-CUL-P 2527 9.2 %
PAR-HERD-S 634 2.3 %
PAR-NEW-IN 13956 50.7 %
PAR-OTHER 1605 5.8 %
PAR-PERSIS 5313 19.3 %
PAR-RAPID 173 0.6 %
SER-FLEXI 450 1.6 %
SER-NSR 7 0.0 %
Total 27513 100.00 %
Figure 1. Number of samples submitted in each category during January 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother
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Breakdown ofSamples
submitted byCounty
Figure 2. Number of samples submitted in each category by County during January 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallelother, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serialpotential fraud, SER-OTHER: serial other
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SER-NSR SER-FLEXI PAR-RAPID PAR-PERSIS PAR-OTHER PAR-NEW-IN PAR-HERD-S PAR-CUL-P
PAR-CUL-N
Breakdownof Samplessubmittedby County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrentJohne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
Table 2a. Summary by County for January 2019
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
England - HRA Avon PAR-PERSIS 2 200 0.73 % 12 6.00 % 169 84.50 % 20 10.00 % 17 8.50 % 2 1.00 %
Corn & Scilly PAR-CUL-N 7 697 2.53 % 27 3.87 % 638 91.54 % 41 5.88 % 32 4.59 % 0 0.00 %
PAR-CUL-P 2 989 3.59 % 17 1.72 % 873 88.27 % 119 12.03 % 97 9.81 % 2 0.20 %
PAR-PERSIS 2 106 0.39 % 1 0.94 % 9 8.49 % 1 0.94 % 1 0.94 % 95 89.62 %
SER-FLEXI 2 15 0.05 % 0 0.00 % 15 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Devon PAR-CUL-N 8 810 2.94 % 65 8.02 % 694 85.68 % 59 7.28 % 50 6.17 % 1 0.12 %
PAR-CUL-P 2 441 1.60 % 18 4.08 % 401 90.93 % 29 6.58 % 22 4.99 % 0 0.00 %
PAR-NEW-IN 1 344 1.25 % 67 19.48 % 242 70.35 % 42 12.21 % 35 10.17 % 0 0.00 %
PAR-PERSIS 3 396 1.44 % 25 6.31 % 330 83.33 % 42 10.61 % 41 10.35 % 0 0.00 %
SER-FLEXI 2 62 0.23 % 6 9.68 % 25 40.32 % 0 0.00 % 0 0.00 % 31 50.00 %
Dorset PAR-CUL-N 5 378 1.37 % 23 6.08 % 314 83.07 % 41 10.85 % 40 10.58 % 1 0.26 %
PAR-CUL-P 2 869 3.16 % 38 4.37 % 795 91.48 % 37 4.26 % 36 4.14 % 0 0.00 %
Gloucs PAR-CUL-N 6 620 2.25 % 30 4.84 % 446 71.94 % 53 8.55 % 44 7.10 % 100 16.13 %
Heref PAR-CUL-N 2 343 1.25 % 15 4.37 % 311 90.67 % 18 5.25 % 17 4.96 % 0 0.00 %
Shropshire PAR-OTHER 2 454 1.65 % 24 5.29 % 392 86.34 % 44 9.69 % 38 8.37 % 0 0.00 %
PAR-PERSIS 1 72 0.26 % 2 2.78 % 56 77.78 % 15 20.83 % 14 19.44 % 0 0.00 %
Somerset PAR-CUL-P 1 228 0.83 % 21 9.21 % 196 85.96 % 17 7.46 % 9 3.95 % 2 0.88 %
PAR-OTHER 2 238 0.87 % 34 14.29 % 155 65.13 % 8 3.36 % 8 3.36 % 41 17.23 %
Wiltshire PAR-OTHER 1 36 0.13 % 1 2.78 % 33 91.67 % 4 11.11 % 2 5.56 % 0 0.00 %
SER-FLEXI 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %
England - Edge Berks PAR-NEW-IN 7 1,009 3.67 % 23 2.28 % 921 91.28 % 72 7.14 % 65 6.44 % 0 0.00 %
PAR-PERSIS 1 92 0.33 % 4 4.35 % 87 94.57 % 2 2.17 % 1 1.09 % 0 0.00 %
Bucks PAR-NEW-IN 1 153 0.56 % 12 7.84 % 138 90.20 % 7 4.58 % 3 1.96 % 0 0.00 %
Cheshire PAR-NEW-IN 23 4,679 17.01 % 108 2.31 % 4172 89.16 % 294 6.28 % 249 5.32 % 150 3.21 %
PAR-PERSIS 1 13 0.05 % 1 7.69 % 12 92.31 % 0 0.00 % 0 0.00 % 0 0.00 %
Derbyshire PAR-NEW-IN 18 2,797 10.17 % 85 3.04 % 2365 84.55 % 154 5.51 % 134 4.79 % 213 7.62 %
E Sussex PAR-NEW-IN 5 807 2.93 % 46 5.70 % 719 89.10 % 30 3.72 % 29 3.59 % 13 1.61 %
Hampshire PAR-NEW-IN 6 55 0.20 % 1 1.82 % 51 92.73 % 3 5.45 % 3 5.45 % 0 0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2a. Summary by County for January 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
SER-NSR 1 7 0.03 % 0 0.00 % 7 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Leics & Rut PAR-NEW-IN 5 475 1.73 % 22 4.63 % 316 66.53 % 54 11.37 % 50 10.53 % 87 18.32 %
Northants PAR-NEW-IN 1 6 0.02 % 0 0.00 % 5 83.33 % 2 33.33 % 1 16.67 % 0 0.00 %
Notts PAR-NEW-IN 3 377 1.37 % 15 3.98 % 259 68.70 % 44 11.67 % 40 10.61 % 63 16.71 %
Oxon PAR-NEW-IN 17 1,858 6.75 % 101 5.44 % 1570 84.50 % 198 10.66 % 187 10.06 % 0 0.00 %
Warks PAR-NEW-IN 10 546 1.98 % 24 4.40 % 428 78.39 % 38 6.96 % 35 6.41 % 59 10.81 %
PAR-PERSIS 2 157 0.57 % 9 5.73 % 137 87.26 % 12 7.64 % 11 7.01 % 0 0.00 %
England - LRA Cambs PAR-NEW-IN 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %
Cumbria PAR-NEW-IN 1 3 0.01 % 0 0.00 % 2 66.67 % 1 33.33 % 1 33.33 % 0 0.00 %
Lancashire PAR-NEW-IN 1 22 0.08 % 0 0.00 % 21 95.45 % 1 4.55 % 1 4.55 % 0 0.00 %
Lincs PAR-NEW-IN 3 60 0.22 % 8 13.33 % 41 68.33 % 11 18.33 % 11 18.33 % 0 0.00 %
S Yorks PAR-NEW-IN 1 162 0.59 % 4 2.47 % 150 92.59 % 10 6.17 % 8 4.94 % 0 0.00 %
England 162 20585 74.82 % 889 4.32 % 17501 85.02 % 1526 7.41 % 1335 6.49 % 860 4.18 %
Wales - High TB Carms PAR-HERD-S 5 455 1.65 % 38 8.35 % 401 88.13 % 24 5.27 % 15 3.30 % 1 0.22 %
PAR-PERSIS 17 2,952 10.73 % 133 4.51 % 2702 91.53 % 139 4.71 % 104 3.52 % 13 0.44 %
PAR-RAPID 11 63 0.23 % 25 39.68 % 34 53.97 % 8 12.70 % 4 6.35 % 0 0.00 %
SER-FLEXI 4 105 0.38 % 6 5.71 % 93 88.57 % 6 5.71 % 6 5.71 % 0 0.00 %
Ceredigion PAR-PERSIS 4 310 1.13 % 17 5.48 % 279 90.00 % 17 5.48 % 14 4.52 % 0 0.00 %
PAR-RAPID 4 5 0.02 % 4 80.00 % 1 20.00 % 1 20.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Gwent PAR-PERSIS 3 392 1.42 % 18 4.59 % 347 88.52 % 31 7.91 % 26 6.63 % 1 0.26 %
PAR-RAPID 8 36 0.13 % 10 27.78 % 22 61.11 % 4 11.11 % 3 8.33 % 1 2.78 %
Pembs PAR-HERD-S 3 49 0.18 % 3 6.12 % 45 91.84 % 1 2.04 % 1 2.04 % 0 0.00 %
PAR-OTHER 5 857 3.11 % 30 3.50 % 767 89.50 % 70 8.17 % 60 7.00 % 0 0.00 %
PAR-PERSIS 6 397 1.44 % 18 4.53 % 357 89.92 % 27 6.80 % 21 5.29 % 1 0.25 %
PAR-RAPID 13 33 0.12 % 7 21.21 % 25 75.76 % 2 6.06 % 1 3.03 % 0 0.00 %
SER-FLEXI 1 3 0.01 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %
Powys PAR-HERD-S 3 3 0.01 % 0 0.00 % 2 66.67 % 0 0.00 % 0 0.00 % 1 33.33 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2a. Summary by County for January 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
PAR-NEW-IN 2 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %
PAR-PERSIS 2 213 0.77 % 31 14.55 % 172 80.75 % 14 6.57 % 10 4.69 % 0 0.00 %
PAR-RAPID 4 34 0.12 % 4 11.76 % 11 32.35 % 2 5.88 % 2 5.88 % 17 50.00 %
SER-FLEXI 1 14 0.05 % 1 7.14 % 13 92.86 % 0 0.00 % 0 0.00 % 0 0.00 %
S Wales PAR-HERD-S 1 3 0.01 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
W Glamorgan PAR-HERD-S 2 117 0.43 % 7 5.98 % 108 92.31 % 5 4.27 % 2 1.71 % 0 0.00 %
PAR-PERSIS 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %
Wales - Intermediate TB Carms PAR-OTHER 1 20 0.07 % 2 10.00 % 12 60.00 % 6 30.00 % 6 30.00 % 0 0.00 %
Ceredigion PAR-HERD-S 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %
NE Wales PAR-NEW-IN 7 515 1.87 % 18 3.50 % 456 88.54 % 37 7.18 % 31 6.02 % 10 1.94 %
PAR-PERSIS 1 6 0.02 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-RAPID 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 8 248 0.90 % 19 7.66 % 166 66.94 % 10 4.03 % 10 4.03 % 53 21.37 %
W Glamorgan PAR-HERD-S 1 6 0.02 % 2 33.33 % 4 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %
Wales - Low TB Gwynedd PAR-NEW-IN 2 28 0.10 % 0 0.00 % 26 92.86 % 1 3.57 % 1 3.57 % 1 3.57 %
NE Wales PAR-NEW-IN 4 46 0.17 % 0 0.00 % 31 67.39 % 1 2.17 % 0 0.00 % 15 32.61 %
Wales 127 6928 25.18 % 394 5.69 % 6098 88.02 % 411 5.93 % 322 4.65 % 114 1.65 %
Grand Total Sum: 289 27513 1283 23599 1937 1657 974
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
England - HRA Avon PAR-PERSIS 2 200 0.73 % 12 6.00 % 169 84.50 % 20 10.00 % 17 8.50 % 2 1.00 %
Corn & Scilly PAR-CUL-N 7 697 2.53 % 27 3.87 % 638 91.54 % 41 5.88 % 32 4.59 % 0 0.00 %
PAR-CUL-P 2 989 3.59 % 17 1.72 % 873 88.27 % 119 12.03 % 97 9.81 % 2 0.20 %
PAR-PERSIS 2 106 0.39 % 1 0.94 % 9 8.49 % 1 0.94 % 1 0.94 % 95 89.62 %
SER-FLEXI 2 15 0.05 % 0 0.00 % 15 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Devon PAR-CUL-N 8 810 2.94 % 65 8.02 % 694 85.68 % 59 7.28 % 50 6.17 % 1 0.12 %
PAR-CUL-P 2 441 1.60 % 18 4.08 % 401 90.93 % 29 6.58 % 22 4.99 % 0 0.00 %
PAR-NEW-IN 1 344 1.25 % 67 19.48 % 242 70.35 % 42 12.21 % 35 10.17 % 0 0.00 %
PAR-PERSIS 3 396 1.44 % 25 6.31 % 330 83.33 % 42 10.61 % 41 10.35 % 0 0.00 %
SER-FLEXI 2 62 0.23 % 6 9.68 % 25 40.32 % 0 0.00 % 0 0.00 % 31 50.00 %
Dorset PAR-CUL-N 5 378 1.37 % 23 6.08 % 314 83.07 % 41 10.85 % 40 10.58 % 1 0.26 %
PAR-CUL-P 2 869 3.16 % 38 4.37 % 795 91.48 % 37 4.26 % 36 4.14 % 0 0.00 %
Gloucs PAR-CUL-N 6 620 2.25 % 30 4.84 % 446 71.94 % 53 8.55 % 44 7.10 % 100 16.13 %
Heref PAR-CUL-N 2 343 1.25 % 15 4.37 % 311 90.67 % 18 5.25 % 17 4.96 % 0 0.00 %
Shropshire PAR-OTHER 2 454 1.65 % 24 5.29 % 392 86.34 % 44 9.69 % 38 8.37 % 0 0.00 %
PAR-PERSIS 1 72 0.26 % 2 2.78 % 56 77.78 % 15 20.83 % 14 19.44 % 0 0.00 %
Somerset PAR-CUL-P 1 228 0.83 % 21 9.21 % 196 85.96 % 17 7.46 % 9 3.95 % 2 0.88 %
PAR-OTHER 2 238 0.87 % 34 14.29 % 155 65.13 % 8 3.36 % 8 3.36 % 41 17.23 %
Wiltshire PAR-OTHER 1 36 0.13 % 1 2.78 % 33 91.67 % 4 11.11 % 2 5.56 % 0 0.00 %
SER-FLEXI 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %
England - Edge Berks PAR-NEW-IN 7 1,009 3.67 % 23 2.28 % 921 91.28 % 72 7.14 % 65 6.44 % 0 0.00 %
PAR-PERSIS 1 92 0.33 % 4 4.35 % 87 94.57 % 2 2.17 % 1 1.09 % 0 0.00 %
Bucks PAR-NEW-IN 1 153 0.56 % 12 7.84 % 138 90.20 % 7 4.58 % 3 1.96 % 0 0.00 %
Cheshire PAR-NEW-IN 23 4,679 17.01 % 108 2.31 % 4172 89.16 % 294 6.28 % 249 5.32 % 150 3.21 %
PAR-PERSIS 1 13 0.05 % 1 7.69 % 12 92.31 % 0 0.00 % 0 0.00 % 0 0.00 %
Derbyshire PAR-NEW-IN 18 2,797 10.17 % 85 3.04 % 2365 84.55 % 154 5.51 % 134 4.79 % 213 7.62 %
E Sussex PAR-NEW-IN 5 807 2.93 % 46 5.70 % 719 89.10 % 30 3.72 % 29 3.59 % 13 1.61 %
Hampshire PAR-NEW-IN 6 55 0.20 % 1 1.82 % 51 92.73 % 3 5.45 % 3 5.45 % 0 0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
SER-NSR 1 7 0.03 % 0 0.00 % 7 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Leics & Rut PAR-NEW-IN 5 475 1.73 % 22 4.63 % 316 66.53 % 54 11.37 % 50 10.53 % 87 18.32 %
Northants PAR-NEW-IN 1 6 0.02 % 0 0.00 % 5 83.33 % 2 33.33 % 1 16.67 % 0 0.00 %
Notts PAR-NEW-IN 3 377 1.37 % 15 3.98 % 259 68.70 % 44 11.67 % 40 10.61 % 63 16.71 %
Oxon PAR-NEW-IN 17 1,858 6.75 % 101 5.44 % 1570 84.50 % 198 10.66 % 187 10.06 % 0 0.00 %
Warks PAR-NEW-IN 10 546 1.98 % 24 4.40 % 428 78.39 % 38 6.96 % 35 6.41 % 59 10.81 %
PAR-PERSIS 2 157 0.57 % 9 5.73 % 137 87.26 % 12 7.64 % 11 7.01 % 0 0.00 %
England - LRA Cambs PAR-NEW-IN 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %
Cumbria PAR-NEW-IN 1 3 0.01 % 0 0.00 % 2 66.67 % 1 33.33 % 1 33.33 % 0 0.00 %
Lancashire PAR-NEW-IN 1 22 0.08 % 0 0.00 % 21 95.45 % 1 4.55 % 1 4.55 % 0 0.00 %
Lincs PAR-NEW-IN 3 60 0.22 % 8 13.33 % 41 68.33 % 11 18.33 % 11 18.33 % 0 0.00 %
S Yorks PAR-NEW-IN 1 162 0.59 % 4 2.47 % 150 92.59 % 10 6.17 % 8 4.94 % 0 0.00 %
England 162 20585 74.82 % 889 4.32 % 17501 85.02 % 1526 7.41 % 1335 6.49 % 860 4.18 %
Wales - High TB Carms PAR-HERD-S 5 455 1.65 % 38 8.35 % 401 88.13 % 24 5.27 % 15 3.30 % 1 0.22 %
PAR-PERSIS 17 2,952 10.73 % 133 4.51 % 2702 91.53 % 139 4.71 % 104 3.52 % 13 0.44 %
PAR-RAPID 11 63 0.23 % 25 39.68 % 34 53.97 % 8 12.70 % 4 6.35 % 0 0.00 %
SER-FLEXI 4 105 0.38 % 6 5.71 % 93 88.57 % 6 5.71 % 6 5.71 % 0 0.00 %
Ceredigion PAR-PERSIS 4 310 1.13 % 17 5.48 % 279 90.00 % 17 5.48 % 14 4.52 % 0 0.00 %
PAR-RAPID 4 5 0.02 % 4 80.00 % 1 20.00 % 1 20.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Gwent PAR-PERSIS 3 392 1.42 % 18 4.59 % 347 88.52 % 31 7.91 % 26 6.63 % 1 0.26 %
PAR-RAPID 8 36 0.13 % 10 27.78 % 22 61.11 % 4 11.11 % 3 8.33 % 1 2.78 %
Pembs PAR-HERD-S 3 49 0.18 % 3 6.12 % 45 91.84 % 1 2.04 % 1 2.04 % 0 0.00 %
PAR-OTHER 5 857 3.11 % 30 3.50 % 767 89.50 % 70 8.17 % 60 7.00 % 0 0.00 %
PAR-PERSIS 6 397 1.44 % 18 4.53 % 357 89.92 % 27 6.80 % 21 5.29 % 1 0.25 %
PAR-RAPID 13 33 0.12 % 7 21.21 % 25 75.76 % 2 6.06 % 1 3.03 % 0 0.00 %
SER-FLEXI 1 3 0.01 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %
Powys PAR-HERD-S 3 3 0.01 % 0 0.00 % 2 66.67 % 0 0.00 % 0 0.00 % 1 33.33 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
PAR-NEW-IN 2 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %
PAR-PERSIS 2 213 0.77 % 31 14.55 % 172 80.75 % 14 6.57 % 10 4.69 % 0 0.00 %
PAR-RAPID 4 34 0.12 % 4 11.76 % 11 32.35 % 2 5.88 % 2 5.88 % 17 50.00 %
SER-FLEXI 1 14 0.05 % 1 7.14 % 13 92.86 % 0 0.00 % 0 0.00 % 0 0.00 %
S Wales PAR-HERD-S 1 3 0.01 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
W Glamorgan PAR-HERD-S 2 117 0.43 % 7 5.98 % 108 92.31 % 5 4.27 % 2 1.71 % 0 0.00 %
PAR-PERSIS 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %
Wales - Intermediate TB Carms PAR-OTHER 1 20 0.07 % 2 10.00 % 12 60.00 % 6 30.00 % 6 30.00 % 0 0.00 %
Ceredigion PAR-HERD-S 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %
NE Wales PAR-NEW-IN 7 515 1.87 % 18 3.50 % 456 88.54 % 37 7.18 % 31 6.02 % 10 1.94 %
PAR-PERSIS 1 6 0.02 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-RAPID 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 8 248 0.90 % 19 7.66 % 166 66.94 % 10 4.03 % 10 4.03 % 53 21.37 %
W Glamorgan PAR-HERD-S 1 6 0.02 % 2 33.33 % 4 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %
Wales - Low TB Gwynedd PAR-NEW-IN 2 28 0.10 % 0 0.00 % 26 92.86 % 1 3.57 % 1 3.57 % 1 3.57 %
NE Wales PAR-NEW-IN 4 46 0.17 % 0 0.00 % 31 67.39 % 1 2.17 % 0 0.00 % 15 32.61 %
Wales 127 6928 25.18 % 394 5.69 % 6098 88.02 % 411 5.93 % 322 4.65 % 114 1.65 %
Grand Total Sum: 289 27513 1283 23599 1937 1657 974
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 3a. Summary of IFN Gamma results by country and protocol January 2019
Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject
n % of total n % n % n % n % n %
England Parallel 156 20499 99.6% 883 4.3% 17453 85.1% 1525 7.4% 1334 6.5% 829 4.0%
Serial/Extended 6 86 0.4% 6 7.0% 48 55.8% 1 1.2% 1 1.2% 31 36.0%
Total 162 20585 100.0% 889 4.3% 17501 85.0% 1526 7.4% 1335 6.5% 860 4.2%
Scotland Parallel 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Total 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Wales Parallel 112 6557 94.6% 367 5.6% 5823 88.8% 395 6.0% 306 4.7% 61 0.9%
Serial/Extended 15 371 5.4% 27 7.3% 275 74.1% 16 4.3% 16 4.3% 53 14.3%
Total 127 6928 100.0% 394 5.7% 6098 88.0% 411 5.9% 322 4.6% 114 1.6%
GB Parallel 268 27056 98.3% 1250 4.6% 23276 86.0% 1920 7.1% 1640 6.1% 890 3.3%
Serial/Extended 21 457 1.7% 33 7.2% 323 70.7% 17 3.7% 17 3.7% 84 18.4%
Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0% 1657 6.0% 974 3.5%
162.00
127.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.
Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject
n % of total n % n % n % n % n %
England Parallel 156 20499 99.6% 883 4.3% 17453 85.1% 1525 7.4% 1334 6.5% 829 4.0%
Serial/Extended 6 86 0.4% 6 7.0% 48 55.8% 1 1.2% 1 1.2% 31 36.0%
Total 162 20585 100.0% 889 4.3% 17501 85.0% 1526 7.4% 1335 6.5% 860 4.2%
Scotland Parallel 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Total 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Wales Parallel 112 6557 94.6% 367 5.6% 5823 88.8% 395 6.0% 306 4.7% 61 0.9%
Serial/Extended 15 371 5.4% 27 7.3% 275 74.1% 16 4.3% 16 4.3% 53 14.3%
Total 127 6928 100.0% 394 5.7% 6098 88.0% 411 5.9% 322 4.6% 114 1.6%
GB Parallel 268 27056 98.3% 1250 4.6% 23276 86.0% 1920 7.1% 1640 6.1% 890 3.3%
Serial/Extended 21 457 1.7% 33 7.2% 323 70.7% 17 3.7% 17 3.7% 84 18.4%
Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0% 1657 6.0% 974 3.5%
162.00
127.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.
Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject
n % of total n % n % n % n % n %
GB
Extended Flexible IFN-GAMMA test 20 450 1.6% 33 7.3% 316 70.2% 17 3.8 % 17 3.8 % 84 18.7 %
Parallel - Herd or Group Slaughter 16 634 2.3% 50 7.9% 563 88.8% 31 4.9 % 19 3.0 % 2 0.3 %
Parallel New Infection 119 13956 50.7% 534 3.8% 11923 85.4% 1004 7.2 % 888 6.4 % 611 4.4 %
Parallel - new infection cull area 28 2848 10.4% 160 5.6% 2403 84.4% 212 7.4 % 183 6.4 % 102 3.6 %
Parallel - Other 11 1605 5.8% 91 5.7% 1359 84.7% 132 8.2 % 114 7.1 % 41 2.6 %
Parallel Persistent Infection 46 5313 19.3% 271 5.1% 4668 87.9% 322 6.1 % 262 4.9 % 112 2.1 %
Parallel - persistent infection cull area 7 2527 9.2% 94 3.7% 2265 89.6% 202 8.0 % 164 6.5 % 4 0.2 %
Parallel - Rapid Testing Two-times IRs 41 173 0.6% 50 28.9% 95 54.9% 17 9.8 % 10 5.8 % 18 10.4 %
Serial - Suspected NSR Herd 1 7 0.0% 0 0.0 % 7 100.0% 0 0 0
Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0 % 1657 6.0 % 974 3.5 %
Table 4a. Summaryof IFN Gamma results by submission reason (January 2019)
England - HRA Extended Flexible IFN-GAMMA test 5 79 0.3% 6 7.6% 41 51.9% 1 1.3 % 1 1.3 % 31 39.2 %
Parallel New Infection 1 344 1.3% 67 19.5% 242 70.3% 42 12.2 % 35 10.2 % 0
Parallel - new infection cull area 28 2848 10.4% 160 5.6% 2403 84.4% 212 7.4 % 183 6.4 % 102 3.6 %
Parallel - Other 5 728 2.6% 59 8.1% 580 79.7% 56 7.7 % 48 6.6 % 41 5.6 %
Parallel Persistent Infection 8 774 2.8% 40 5.2% 564 72.9% 78 10.1 % 73 9.4 % 97 12.5 %
Parallel - persistent infection cull area 7 2527 9.2% 94 3.7% 2265 89.6% 202 8.0 % 164 6.5 % 4 0.2 %
England - HRA 54 7300 26.5% 426 5.8% 6095 83.5% 591 8.1 % 504 6.9 % 275 3.8 %
England - Edge Parallel New Infection 96 12762 46.4% 437 3.4% 10944 85.8% 896 7.0 % 796 6.2 % 585 4.6 %
Parallel Persistent Infection 4 262 1.0% 14 5.3% 236 90.1% 14 5.3 % 12 4.6 % 0
Serial - Suspected NSR Herd 1 7 0.0% 0 0.0 % 7 100.0% 0 0 0
England - Edge 101 13031 47.4% 451 3.5% 11187 85.8% 910 7.0 % 808 6.2 % 585 4.5 %
England - LRA Parallel New Infection 7 254 0.9% 12 4.7% 219 86.2% 25 9.8 % 23 9.1 % 0
England - LRA 7 254 0.9% 12 4.7% 219 86.2% 25 9.8 % 23 9.1 % 0
Wales - High TB Extended Flexible IFN-GAMMA test 7 123 0.4% 8 6.5% 109 88.6% 6 4.9 % 6 4.9 % 0
Parallel - Herd or Group Slaughter 14 627 2.3% 48 7.7% 559 89.2% 30 4.8 % 18 2.9 % 2 0.3 %
Parallel New Infection 2 7 0.0% 0 0.0 % 5 71.4% 2 28.6 % 2 28.6 % 0
Parallel - Other 5 857 3.1% 30 3.5% 767 89.5% 70 8.2 % 60 7.0 % 0
Parallel Persistent Infection 33 4271 15.5% 217 5.1% 3862 90.4% 230 5.4 % 177 4.1 % 15 0.4 %
Parallel - Rapid Testing Two-times IRs 40 171 0.6% 50 29.2% 93 54.4% 17 9.9 % 10 5.8 % 18 10.5 %
Wales - High TB 101 6056 22.0% 353 5.8% 5395 89.1% 355 5.9 % 273 4.5 % 35 0.6 %
Wales - Intermediate TB Extended Flexible IFN-GAMMA test 8 248 0.9% 19 7.7% 166 66.9% 10 4.0 % 10 4.0 % 53 21.4 %
Parallel - Herd or Group Slaughter 2 7 0.0% 2 28.6% 4 57.1% 1 14.3 % 1 14.3 % 0
Parallel New Infection 7 515 1.9% 18 3.5% 456 88.5% 37 7.2 % 31 6.0 % 10 1.9 %
Parallel - Other 1 20 0.1% 2 10.0% 12 60.0% 6 30.0 % 6 30.0 % 0
Parallel Persistent Infection 1 6 0.0% 0 0.0 % 6 100.0% 0 0 0
Parallel - Rapid Testing Two-times IRs 1 2 0.0% 0 0.0 % 2 100.0% 0 0 0
Wales - Intermediate TB 20 798 2.9% 41 5.1% 646 81.0% 54 6.8 % 48 6.0 % 63 7.9 %
Wales - Low TB Parallel New Infection 6 74 0.3% 0 0.0 % 57 77.0% 2 2.7 % 1 1.4 % 16 21.6 %
Wales - Low TB 6 74 0.3% 0 0.0 % 57 77.0% 2 2.7 % 1 1.4 % 16 21.6 %
Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject
n % of total n % n % n % n % n %
GB
Extended Flexible IFN-GAMMA test 20 450 1.6% 33 7.3% 316 70.2% 17 3.8 % 17 3.8 % 84 18.7 %
Parallel - Herd or Group Slaughter 16 634 2.3% 50 7.9% 563 88.8% 31 4.9 % 19 3.0 % 2 0.3 %
Parallel New Infection 119 13956 50.7% 534 3.8% 11923 85.4% 1004 7.2 % 888 6.4 % 611 4.4 %
Parallel - new infection cull area 28 2848 10.4% 160 5.6% 2403 84.4% 212 7.4 % 183 6.4 % 102 3.6 %
Parallel - Other 11 1605 5.8% 91 5.7% 1359 84.7% 132 8.2 % 114 7.1 % 41 2.6 %
Parallel Persistent Infection 46 5313 19.3% 271 5.1% 4668 87.9% 322 6.1 % 262 4.9 % 112 2.1 %
Parallel - persistent infection cull area 7 2527 9.2% 94 3.7% 2265 89.6% 202 8.0 % 164 6.5 % 4 0.2 %
Parallel - Rapid Testing Two-times IRs 41 173 0.6% 50 28.9% 95 54.9% 17 9.8 % 10 5.8 % 18 10.4 %
Serial - Suspected NSR Herd 1 7 0.0% 0 0.0 % 7 100.0% 0 0 0
Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0 % 1657 6.0 % 974 3.5 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA Extended Flexible IFN-GAMMA test 5 79 0.3% 6 7.6% 41 51.9% 1 1.3 % 1 1.3 % 31 39.2 %
Parallel New Infection 1 344 1.3% 67 19.5% 242 70.3% 42 12.2 % 35 10.2 % 0
Parallel - new infection cull area 28 2848 10.4% 160 5.6% 2403 84.4% 212 7.4 % 183 6.4 % 102 3.6 %
Parallel - Other 5 728 2.6% 59 8.1% 580 79.7% 56 7.7 % 48 6.6 % 41 5.6 %
Parallel Persistent Infection 8 774 2.8% 40 5.2% 564 72.9% 78 10.1 % 73 9.4 % 97 12.5 %
Parallel - persistent infection cull area 7 2527 9.2% 94 3.7% 2265 89.6% 202 8.0 % 164 6.5 % 4 0.2 %
England - Edge Parallel New Infection 96 12762 46.4% 437 3.4% 10944 85.8% 896 7.0 % 796 6.2 % 585 4.6 %
Parallel Persistent Infection 4 262 1.0% 14 5.3% 236 90.1% 14 5.3 % 12 4.6 % 0
Serial - Suspected NSR Herd 1 7 0.0% 0 0.0 % 7 100.0% 0 0 0
England - LRA Parallel New Infection 7 254 0.9% 12 4.7% 219 86.2% 25 9.8 % 23 9.1 % 0
England 162 20585 74.8% 889 4.3% 17501 85.0% 1526 7.4 % 1335 6.5 % 860 4.2 %
Wales - High TB Extended Flexible IFN-GAMMA test 7 123 0.4% 8 6.5% 109 88.6% 6 4.9 % 6 4.9 % 0
Parallel - Herd or Group Slaughter 14 627 2.3% 48 7.7% 559 89.2% 30 4.8 % 18 2.9 % 2 0.3 %
Parallel New Infection 2 7 0.0% 0 0.0 % 5 71.4% 2 28.6 % 2 28.6 % 0
Parallel - Other 5 857 3.1% 30 3.5% 767 89.5% 70 8.2 % 60 7.0 % 0
Parallel Persistent Infection 33 4271 15.5% 217 5.1% 3862 90.4% 230 5.4 % 177 4.1 % 15 0.4 %
Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Parallel - Rapid Testing Two-times IRs 40 171 0.6% 50 29.2% 93 54.4% 17 9.9 % 10 5.8 % 18 10.5 %
Wales - Intermediate TB Extended Flexible IFN-GAMMA test 8 248 0.9% 19 7.7% 166 66.9% 10 4.0 % 10 4.0 % 53 21.4 %
Parallel - Herd or Group Slaughter 2 7 0.0% 2 28.6% 4 57.1% 1 14.3 % 1 14.3 % 0
Parallel New Infection 7 515 1.9% 18 3.5% 456 88.5% 37 7.2 % 31 6.0 % 10 1.9 %
Parallel - Other 1 20 0.1% 2 10.0% 12 60.0% 6 30.0 % 6 30.0 % 0
Parallel Persistent Infection 1 6 0.0% 0 0.0 % 6 100.0% 0 0 0
Parallel - Rapid Testing Two-times IRs 1 2 0.0% 0 0.0 % 2 100.0% 0 0 0
Wales - Low TB Parallel New Infection 6 74 0.3% 0 0.0 % 57 77.0% 2 2.7 % 1 1.4 % 16 21.6 %
Wales 127 6928 25.2% 394 5.7% 6098 88.0% 411 5.9 % 322 4.6 % 114 1.6 %
Table 5a. Summary of PM and culture results for IFNg positive animals.
Year Month Herdssampled
Samplestested
Herdswithpositives
IFNg+samples
WrongEartags
No PMNoCult
No PMCultPend
No PMCultNeg
No PMCultMb
NoPMCultOther
VL NoCult
VLCultNeg
VLCultPend
VLCultMb
VLCultOther
NVLNoCult
NVLCultPend
NVLCultNeg
NVLCultMb
NVLCultOther
2018 February 206 20791 130 1229 3 22 0 0 0 0 72 7 1 8 0 1073 1 35 0 5
March 189 17292 131 1199 1 3 0 0 0 0 121 6 0 4 0 984 0 72 1 2
April 195 20517 118 953 4 16 0 0 0 0 116 1 0 0 0 748 0 66 1 1
May 250 21066 131 947 2 2 0 0 0 0 121 1 0 4 0 780 0 35 1 1
June 187 18891 110 701 2 2 0 0 0 0 78 2 0 12 0 579 0 25 0 0
July 207 24111 119 806 1 12 0 0 0 0 67 4 0 2 0 675 3 38 0 2
August 228 19599 120 682 4 3 0 0 0 0 64 2 0 8 1 569 0 31 0 0
September 216 21441 137 946 6 6 0 0 0 0 33 1 0 2 0 840 0 51 1 4
October 274 23976 152 1417 12 8 0 0 0 0 99 1 0 3 1 1231 5 40 0 1
November 282 24570 192 1580 2 5 0 0 0 0 42 0 4 7 4 1329 84 87 0 7
December 228 21554 147 1241 6 21 0 0 0 0 91 0 8 2 0 1025 87 0 0 0
2019 January 289 27513 179 1283 474 412 0 0 0 0 26 0 3 0 0 237 14 0 0 0
Totals (last12 months) 2751 261321 1666 12984 517 512 0 0 0 0 930 25 16 52 6 10070 194 480 4 23
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)
Country Herdssampled
Samplestested
Herdswithpositives
IFNg+samples
No PMNoCult
No PMCultPend
No PMCultNeg
No PMCultMb
NoPMCultOther
VL NoCult
VLCultNeg
VLCultPend
VLCultMb
VLCultOther
NVLNoCult
NVLCultPend
NVLCultNeg
NVLCultMb
NVLCultOther
England 1530 200003 1049 9852 271 0 0 0 0 775 15 7 34 4 8193 25 51 1 3
Scotland 26 1828 17 146 30 0 0 0 0 22 4 0 0 0 49 0 36 0 3
Wales 1195 59490 600 2986 211 0 0 0 0 133 6 9 18 2 1828 169 393 3 17
Totals 2751 261321 1666 12984 512 0 0 0 0 930 25 16 52 6 10070 194 480 4 23
Figure 4a. Number of herds sampled for IFNg and numbers of herds with anypositive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
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81
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97
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108
33
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67
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88
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JanuaryFebruaryM
archAprilM
ayJuneJulyAugustSeptem
berO
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archAprilM
ayJuneJulyAugustSeptem
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2019
IFNg Positive herds IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
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10097
560
10063
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19186
1229
15561
1199
18776
953
19795
947
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701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
0
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30000
January
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January
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2017
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2019
IFNg positive animals IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals withpositive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes
0
1
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Janu
ary
Febr
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ch
April
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%Rejected %Resampled % NEG Fail % POS Fail
% of totalsubmitted
Figure 5. Rates of resamples including the fail rates broken down for positive(POS) and negative (NEG) controls.
0 10 20 30 40 50 60 70
Wiltshire
Warks
S Wales
Shropshire
Pembs
Notts
NE Wales
Leics & Rut
Heref
Gwynedd
Gloucs
Dorset
Derbyshire
Corn & Scilly
Ceredigion
Cambs
Berks
%Retested %Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during January 2019 (% submission perCounty) County %Resampled %Retested
Avon 8.50 % 10.00 %
Berks 5.99 % 6.72 %
Bucks 1.96 % 4.58 %
Cambs 28.57 % 28.57 %
Carms 3.76 % 5.09 %
Ceredigion 4.73 % 5.99 %
Cheshire 5.31 % 6.27 %
Corn & Scilly 7.19 % 8.91 %
Cumbria 33.33 % 33.33 %
Derbyshire 4.79 % 5.51 %
Devon 7.21 % 8.38 %
Dorset 6.09 % 6.26 %
E Sussex 3.59 % 3.72 %
Gloucs 7.10 % 8.55 %
Gwent 6.78 % 8.18 %
Gwynedd 3.57 % 3.57 %
Hampshire 4.84 % 4.84 %
Heref 4.96 % 5.25 %
Lancashire 4.55 % 4.55 %
Leics & Rut 10.53 % 11.37 %
Lincs 18.33 % 18.33 %
NE Wales 5.02 % 5.88 %
Northants 16.67 % 33.33 %
Notts 10.61 % 11.67 %
Oxon 10.06 % 10.66 %
Pembs 6.20 % 7.47 %
Powys 5.17 % 6.64 %
Shropshire 9.89 % 11.22 %
Somerset 3.65 % 5.36 %
S Wales
S Yorks 4.94 % 6.17 %
Warks 6.54 % 7.11 %
W Glamorgan 3.08 % 5.38 %
Wiltshire 7.89 % 13.16 %
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 20087 889 4.4% 20585 889 4.3%
Wales 6848 394 5.8% 6928 394 5.7%
GB 26935 1283 4.8% 27513 1283 4.7%
Table 6a (i): Number of animals and submissions tested in January 2019
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 20087 889 4.4% 20585 889 4.3%
Wales 6848 394 5.8% 6928 394 5.7%
GB 26935 1283 4.8% 27513 1283 4.7%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of January
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 14147 657 4.6% 14220 657 4.6%
Wales 4813 277 5.8% 5045 277 5.5%
Scotland 410 35 8.5% 413 35 8.5%
GB 19370 969 5.0% 19678 969 4.9%
Table 6a (ii): Number of animals and submissions tested in January 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of January
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 14147 657 4.6% 14220 657 4.6%
Wales 4813 277 5.8% 5045 277 5.5%
Scotland 410 35 8.5% 413 35 8.5%
GB 19370 969 5.0% 19678 969 4.9%
* Test type NumSubmissions Num Samples Num Positives
Private % Positive
Gloucestershire PRV-SP 1 4 0
Somerset excl North PRV-SE 1 4 1 25.00 %
Wiltshire PRV-SP 1 3 0
Total 3 11 1 9.09 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;PRV-SP: Private High Specificity test – England
Table 7a Number of private samples January 2019
Table 7b Number of private samples 2019
* Test type NumSubmissions
NumSamples
NumPositivesPrivate
% Positive
Gloucestershire PRV-SP 1 4 0
Somerset excl North PRV-SE 1 4 1 25.00 %
Wiltshire PRV-SP 1 3 0
Total 3 11 1 9.09 %
Isle of Man SER-IOM 3 19 0
Isle of Man SER-IOM 3 19 0
Test County Num Submissions Num Samples Tested Num Positive Samples % Positive
TC0077 North East Wales 2 97 5 5.2 %TC0077 Sum: 97 5 5.2 %
Table 8a Number of Cattle Antibody Tests January 2019
Test County Num Submissions Num Samples Tested Num Positive Samples % Positive
TC0077 North East Wales 2 97 5 5.2 %TC0077 Sum: 97 5 5.2 %
Table 8b Number of Cattle Antibody Tests 2019