monsanto 08-23-05b
-
Upload
finance28 -
Category
Economy & Finance
-
view
427 -
download
0
description
Transcript of monsanto 08-23-05b
Corn Breeding OverviewAnkeny Investor Field Day
August 22, 2005Mark Messmer, Corn Commercial Breeding Lead
Monsanto Company
2
MON Corn Breeding Keys To Success
Line Review Worksheet
Show selected lines only ExportLines Nursery notes
Select
YLD % of Check Mean Keep Plot Lineage Trait 1 Trait 2 Trait 3 Comments
x 110 14011 :@>0003.1001.4001. 7 5 22 nice95 X 14012 :@>0003.1001.4002. 6 6 24 better85 Breeder Comments
x 120 Advance this line into PCM1 on 6 testers
101x 105
87Data GCA SCA
Line Data Double click entry to show Set data sheetSelect Set Entry Tester Gen # Loc SELIN YLD MST TWT YM RTLP STLP PHT EHT CZ INT FSP DRP ERM
GCA All 39 120 120 105 97 120 220 105 100 75 75 100 114X C7201 5 19HGZ1 F4 13 140 250 18.5 56.3 14 15 115 53 1 99 113
C8202 4 80IDM2 F4 13 103 229 19.1 56.7 12 2 116 44 3 4 100 114X C8207 12 LH283 F4 13 113 226 19 57 12 1 118 42 5 4 100 115
C8101 26 :@>0003+ F3 7 132 241 18 56 13 6 112 51 2 99 113
Rank Data Ranks DataSet Entry Tester Overall Loc 1 Loc 2 Loc 3 Loc 4 Loc 5 Loc 6 Loc 7 Loc 8 Loc 9 Loc 10 Loc 11 Loc 12 Loc 13 Loc 14 Loc 15
Mean Yield 140 150 160 165 170 175 180 185 190 200 210 215 300C7201 5 19HGZ1 1 45 55 66 33 20 5 4 4 3 2 2 1 2C8202 4 80IDM2 18 50 66 33 21 30 40 5 6 7 8 2 1 3C8207 12 LH283 16 60 12 20 30 8 8 5 4 10 12 2 8 4
C7201 5 19HGZ1 2 1 5 8 25 47 36 22 1 1 5 4 3 8C8202 4 80IDM2 4 2 5 22 44 56 12 11 5 8 22 14 16 55C8207 12 LH283 16 14 22 11 10 1 17 27 26 5 4 9 8 7
08_01DKD2*2/7640:@>0126.1001.08_01DKD2*2/7640:@>0160.1002.08_01DKD2*2/7640:@>0171.1003.
08_01DKD2*2/7640:@>0003.1001.
Pedigree08_01DKD2*2/7640:@>0003.1001.08_01DKD2*2/7640:@>0102.1001.
08_01DKD2*2/7640:@>0175.1002.
Checks Check mean08_01DKD2*2/7640:@>0179.1003.
Add Filter?
Select Below and update above?
Sort by Yield Levels
Green - Hybrid above chk mRed - Hybrid below chk mea
• Structured to support commercial strategy
– Multiple channels
• Automating the Breeding Process
– Driving experimental product numbers game
• Germplasm Diversity– Broad and deep
• Marker Assisted Line Development
– An SOP Enhancing the probability of breeding success
• Pipeline Depth– More improved products
3
00.10.20.30.40.50.60.70.80.9
1
Breeding TechnologyLine Development
Commercial Development Linkages andExchange of informationAre key to Success.
NewTechnology
LINEDEVELOPMENT
Breeding
BREEDINGTECHNOLOGY
COMMERCIAL BREEDING
• Marker Labs• Statistics• Disease and Insect• Winter Nurseries• Discovery Breeding
• F1 to national testing
• Quality grain programs
• Product Characterization
• Channel Tech Support
• Testing• QA/QC
Corn Breeding Structure is Designed to Support MultipleCommercial Channels
PRO
DU
CT
MAN
AGEM
ENT
CHANNEL DEPLOYMENT
• Channel Strategy• Channel Needs• Comm Development
• MON Brands• National Partners• Regional Partners• Local Partners
4
Regional Breeding Teams Tie Breeding Functions Together
NorthNorth
W. CentralW. Central E. CentralE. Central
SouthSouthRegional Breeding Team Objectives:
1. Focus on most elite germplasm
2. Maintain important germplasm types
3. Enhance Product diversity
4. Enhance breeder germplasm knowledge and info exchange
5. Breeder synergy to identify and support customer needs
5
Expansive breeding and testing geography enables effective product identification and placement
6
Shelling Grading Waste Discard
Color Sorting
Culls and KeepsSeed TreatmentFinished Product Ready to Packet
State of the Art Automated Experimental Seed Processing Sets the Stage for Outstanding Field Data Quality
7
Investment in State of the Art Field Plot Equipment Enables Direct Mimicking of Farmer Practices and Timing
~50% Increase in Plot Testing Capacity Over the Past Two Years
8
Plant Breeding: What do we actually do?
• Plant Breeding – is the science, art, and businessof improving plants for human benefit.
• Easy Description– Cross good germplasm by good germplasm– Generate a series of genetically different exptl. parents– Pick the right tester – Select the best experimental line via extensive field
testing• More complicated Description
delta(G)=1/C(0.25*k1*V(a)1.2/sqrt(V(P)1.2) + 0.25*k2*V(a)2.1/sqrt(V(P))2.1)
9
Vast germplasm resources enable deployment of differentiated product portfolios
Holden’s
DEKALBUSA
Asgrow USA
Cargill Int’l
Sensako Agroceres
DEKALB Int’l
AsgrowInt’l
50% of breeding pop’n are “inter-company” crosses
Inter-companyCrosses
12 countries and 3 companies/country = 36 major germplasm acquisitions
10
Yield from Parent 2Yield from Parent 1
1 2 5 6 7 8 9 103 4
Corn has many thousands of genes, hundreds of which probably affect yield
Over 3 billion different genotypes result from just 2 forms of each of 20 genes
The Dilemma:Germplasm Sources X Genes
Marker-Assisted Recurrent Selection (MARS) rapidly increases the frequency of favorable genes
TissueSampling
DNA extraction
Generate Marker Fingerprint
Data Analysis
Selection and Recombination
GenotypingLab
Breeder
Multiple cycles of MARS increases the frequency of favorable marker alleles associated with agronomic traits
Multiplecycles
0.4
0.5
0.6
0.7
0.8
0.9
1.0
Freq
uenc
y of
Fav
orab
le G
ene
C0 C1 C2 C3
12
Marker Assisted Breeding:Sorting the Germplasm and Genes…A Vastly Enhanced Numbers Game
Frequency of:Cycle of Selection
A Favorable Allele
The Best Genotype
0 0.51 2 per tr illion1 0.62 5 per billion2 0.81 1 per 50003 0.96 1 in 5
*Assumes 20 independent regions and 5% selection intensity
Start
End
Yield from Parent 2Yield from Parent 1
Mapping QTL position, size, and favorable parent
13
RUBICON: Integrate and Analyze Field and Marker data
DataData Decisions
Breeders Build an Optimized Genetic Model for Every Breeding Population and use MARS to Toward the Model
Select Traits
Quality metricsPredicted Model Response
Parental Contribution
Identify and Select QTLs
14
Year 1
Marker Characterization Early in the Breeding Enhances Probability of Success Late in the Process
Recombine genes
Select best based on phenotype
Yield test
Select best
Yield test
Commercialize
X6-to 8-year
process6-to 8-year
process
Years 2-6
Year 7 Commercial product success rate < 1%
Commercial product success rate < 1%
Marker Marker CharacterizationCharacterization
MarkerMarker--Assis
tedAssis
ted
Recurrent Selection
Recurrent Selection
120,000+
10’s
Genotypic and phenotypic selection of parents
15
Marker Assisted Breeding Drives Increased Genetic Gain
MTI = Multiple Trait Index that combines multiple traits like grain yield, grain moisture, standability, and test weight.
0.00.20.40.60.81.01.21.41.61.8
MTI
val
ue (P
aren
t = 0
)
2002 2003 2004 All Years
Conventional Markers
2x Improvement
16
MON New 2005 Commercials Competitive Leader HybridsMON 2005 PCM4sMON Brand Leader hybridsDenotes YG hybrid
85
90
95
100
105
110
115
120
DK53
7
DKC57
-01
NA56
05NRR1
NB55
01
NB59
01
NC53
01
NC53
05
NC53
08
NC53
10
NC54
06
NB54
05
NC55
02
NC56
07
NC57
02
NC57
06
PION36
B08
PION35
Y54
PION35
Y65
85
90
95
100
105
110
115
120
DKC51
-43
DK53
7
NB46
02
NC48
01
NB51
01NRR1
NC52
05
NC52
08
NC52
09
NC52
10
NB51
01
PION38
H67
PION36
N70
85
90
95
100
105
110
115
120
DK44
0
DKC46
-26
DKC44
-46
DKC47
-10
NC45
04
NC47
02
NC45
05
NC45
01EZ
A1
NB46
01
NB47
03
NC46
03
NC49
02
PION38
A24
PION38
A25
85
90
95
100
105
110
115
120
DKC60-15
RX7
40
DKC60-19
RX7
18RR/YG
NC6104
NC5801
NC5802
NC5808
NC5809
NC5902
NC5903
NC5907
NC5910
PION34H31
PION34M94
PION34M95
PION33N44
`
95 RM 100 RM
105 RM 108-109 RM
A full pipeline enables continued support of growth via the commercial strategy (95 - 109 RM)
17
MON New 2005 Commercials
Competitive Leader Hybrids
MON 2005 PCM4s
MON Brand Leader hybrids
Denotes YG hybrid
110-112 RM
116-117 RM
113-115 RM
85
90
95
100
105
110
115
120
DKC60
-15
RX7
40
DKC60
-19
RX7
18RR/YG
NB61
04
NB61
06
NC60
09
NC62
10
NC62
13
NC62
14
NC62
15EZ
A1
PION34
H31
PION34
M94
PION34
M95
PION33
N44
`
85
90
95
100
105
110
115
120
DK
C63
-50
DK
C63
-79
DK
C63
-78
DK
C64
-10
NB
6402
NR
R1
NB
6504
EZA
1
NC
6304
NC
6307
NC
6313
NC
6315
NC
6403
NC
6407
NC
6505
NC
6509
NC
6510
PIO
N33
B50
PIO
N33
D31
PIO
N33
B51
PIO
N33
P67
New commercial and experimental products appear ready to allow growth in later RMs (110 - 117 RM)
85
90
95
100
105
110
115
120
DKC63
-50
DKC63
-79
DKC63
-78
DKC64
-10
NB66
02EZ
A1
NC66
07
NC66
08
NC66
09
NC66
11EZ
A1
PIO
N31
G98
PIO
N33
D31
PIO
N33
B51
PIO
N33
P67
18
Invention is the Best Way to Predict the Future
(10 cM between marks)
(5 lo
g(pv
alue
) uni
ts b
etw
een
mar
ks)
Yield
Test Weight
Total DamagedPlantsStay Green
RindPenetrometerProtogyny
Plant Height
Moisture
Intactness
GDU pollenshedEar Height
Gray Leaf Spot
Chrom
Markers