Rapid Molecular Detection of

Tuberculosis and Rifampin

Resistance.

Presented By : Khaled Alkhodari

Supervised By :Dr. saeb aliwaini

Due to : 26/11/2014

Outline

I. Introduction.

II. Methods.

III. Results.

IV. Conclusion.

2

Introduction

Tuberculosis (TB): is a bacterial infection that can spread through the lymph

nodes and bloodstream to any organ in the body. It is most often found in the

lungs.

Rifampin : is a drug used to treat TB

inhibits bacterial RNA polymerase.

Bacterial resistance to it is caused by mutations in rpoB gene leading to a change

in the structure of the β subunit of RNA polymerase.

3

Methods

Xpert MTB/RIF : an automated molecular test for Mycobacterium

tuberculosis(MTB) and resistance to rifampin (RIF).

Three sputum specimens:

Two specimens were processed with:

N-acetyl-l-cysteine ( NALC ) and sodium hydroxide (NaOH ) before microscopy, solid and

liquid culture.

The MTB/RIF test.

One specimen was used for direct testing with microscopy and the MTB/RIF test.

4

Xpert MTB/RIF Test

5

an automated molecular test for

Mycobacterium tuberculosis(MTB) and

resistance to rifampin (RIF),

polymerasechain-reaction (PCR) assay to

amplify an MTB specific sequence of the

rpoB gene , gene encodes the β subunit of

bacterial RNA-polymerase.

6

(Fig. 1)

7

Results

(Fig. 1 Cont’d)

8

9

10

Conclusion

The MTB/RIF test provided sensitive detection of tuberculosis and rifampin

resistance directly from untreated sputum in less than 2 hours with minimal

hands-on time.

11

12

The authors

Catharina C. Boehme, M.D., Pamela Nabeta, M.D., Doris Hillemann, Ph.D.,

Mark P. Nicol, Ph.D.,

Shubhada Shenai, Ph.D., Fiorella Krapp, M.D., Jenny Allen, B.Tech., Rasim

Tahirli, M.D., Robert Blakemore, B.S.,

Roxana Rustomjee, M.D., Ph.D., Ana Milovic, M.S., Martin Jones, Ph.D., Sean

M. O’Brien, Ph.D.,

David H. Persing, M.D., Ph.D., Sabine Ruesch-Gerdes, M.D., Eduardo Gotuzzo,

M.D., Camilla Rodrigues, M.D.,

David Alland, M.D., and Mark D. Perkins, M.D.

13