Molecular Biology Products - POLATOM · patented Tyramide Signal Amplification (TSA ™)...

Molecular Biology Products

PerkinElmer has a complete selection of labeling and detection reagents for your research.

Our Always Fresh NEN Radionucleotides, chemiluminescent and chromogenic reagents,

and membranes and films provide the tools you need for traditional molecular biology

applications. Our exclusive TSA signal amplification technology provides unsurpassed

sensitivity for immunohistochemistry and in situ hybridization. Plus, we offer

a broad range of fluorescent- and hapten-labeled nucleotide analogs, SNP

detection systems, and nucleic acid labeling kits for microarray and other genomic

applications. And now, access advanced array technology for Comparative

Genomic Hybridization (CGH) with our Spectral Genomics products.

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Contents

Technology Overview: Spectral Genomics Array Comparative Genomic Hybridization 238

Technology Showcase: Tyramide Signal Amplification 239

Nucleic Acid Labeling & Detection 241Radiolabeled Nucleotides 241

Radionucleotides Application Guide 242

Nucleic Acid Labeling Systems 243

Hybridization Probes 243

Enzymes 243

Chemiluminescence & Chromogenic Substrates (DNA Thunder) 244

Protein Labeling & Detection 245Western Blotting Detection Options 245

Chemiluminescence & Chromogenic Substrates (Western Lightning) 245

NitroGlo Nitrosylation Kit 246

Protein Molecular Weight Markers 247

Recombinant 125I-Labeled Protein A and Protein G 248

Conjugated Secondary Antibodies 249

Blotting & Autoradiography 249Hybridization Transfer Membranes (GeneScreen, GeneScreen Plus, Colony/Plaque Screen, PolyScreen, Protran®) 249

KODAK™ Autoradiography Films, Screens & Accessories 252

Immunohistochemistry & In Situ Hybridization (TSA) 255

Nucleotide Analogs & cDNA Microarray Analysis 259Fluorescent & Hapten-Labeled Nucleotide Analogs 259

MICROMAX Labeling Systems 264

Array Comparative Genomic Hybridization (aCGH, Spectral Genomics) 265

SNP Detection Systems 266

Technology OverviewM

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ts PerkinElmer Spectral Genomics Products for ArrayComparative Genomic Hybridization (aCGH)

Generate high resolution molecular karyotypes in a single experiment with PerkinElmer’s array CGH technology and software from Spectral Genomics.Chromosomal abnormalities (deletions, duplications, and amplifications) are traditionally determined using whole chromosome preparations from cells (metaphase spreads). These are analyzed using G banding, FISH or by chromosomal Comparative Genomic Hybridization (CGH).

These traditional methods for detecting chromosomal abnormalities are well established, but they do not provide the speed, throughput, or resolution required for today’s cytogenetic laboratories. They are also time consuming and cumbersome, and require the skills of a trained cytogeneticist to analyze and interpret.

Rapid Analysis with High ResolutionPerkinElmer’s Spectral Genomics array CGH technology(aCGH) circumvents the considerable limitations of usingmetaphase spreads and takes karyotyping to a new level of performance and ease-of-use.

Traditional array-based methods for detecting genomic variation, such as SNP and oligo arrays often yield resultsthat are noisy, or unclear. Our Spectral GenomicsSpectralChip™ 2600 BAC arrays provide researchers with high quality results that facilitate genetic disease research.Because each BAC is printed in duplicate, and a dye swapreaction is performed, you obtain reliable results.

The SpectralChip 2600 allows investigators to rapidly pan for chromosomal biomarkers across the entire humangenome. You can generate a quantitative genome-widemolecular profile of chromosomal imbalances on a singlechip. Research applications* include:

• Molecular karyotyping: research congenital and acquiredgenetic changes.

• Biomarker discovery: identify chromosomal abnormalitiesthat are early stage predictors of certain cancers.

• Develop molecular fingerprints for specific tumors andtheir subtypes.

• Identify novel chromosomal regions for potential drug targeting.

* For Research Use Only. Not for use in diagnostic procedures.

Features & Benefits• Conduct high-resolution, high sensitivity molecular

karyotyping: research various cytogenetic abnormalitiesthat may exist as deletions, duplications, amplifications,or cryptic imbalances such as telomere anomalies. Theincreased sensitivity of our technology provides the capa-bility to study low level mosaic changes in the genome.

• Low background: facilitates data with very little noise,providing the clearest array results available today.

• Detect changes that occur throughout the entire genome inone test: our human arrays contain thousands of clones.

• DNA is analyzed directly by hybridization: no prior amplification of sample DNA.

• No harvesting of cells is required before analysis of chromosomal abnormalities, unlike conventionalmetaphase spread techniques for karyotyping.

• Analyze samples from which chromosome preparationsare difficult or impossible.

For details about our Spectral Genomics products,please see p. 265.

Our Spectral Genomicsmicroarray platformprovides the benefitsof the whole genomeperspective of karyotyping, with the increased resolution of FISH and the methodologyof CGH.

Increase your detection capabilities with the outstanding sensitivity enhancements of our proprietary,patented Tyramide Signal Amplification (TSA™) technology. TSA enhances the results of many detection systems, especially standard immunohistochemistry (IHC) and in situ hybridization (ISH) protocols.

Tyramide Signal Amplification (TSA)

The Ultimate Technology for Maximizing Detection Sensitivity

Technology Showcase

Invented by PerkinElmer scientists, TSAcan be used in any application allowingthe addition of horseradish peroxidase(HRP) into its protocol, such as immuno-histochemistry, in situ hybridization,ELISA and microarray-based differentialgene and protein expression studies.

In standard IHC and ISH protocols, TSAamplifies both chromogenic and fluores-cent signals, resulting in a significantincrease in sensitivity, without loss of resolution or increase in background. Use it in immunohistochemistry or ELISAapplications, and unamplified detectionlevels can be maintained while using upto 1,000-fold less primary antibody.

Innovative Amplification ApproachMaintains Signal Clarity

TSA is easily integrated into any protocolafter an initial addition of horseradishperoxidase (HRP). The HRP is used to catalyze the deposition and binding of alabeled (e.g., biotin, DNP or other labelingmoieties) tyramide onto tissue sections orcell preparations previously blocked withproteins. This reaction is quick (less than10 minutes). The binding is covalentbecause the reaction intermediatelydimerizes with tyrosine residues on thesurface-bound endogenous proteins. Theselabels can then be detected by standardchromogenic or fluorescent techniques.Since the added labels are only depositedproximal to the enzyme site, there is minimal, if any, loss of resolution.

Use Versatile, Flexible TSA in Many Applications

Immunohistochemistry: amplify chromogenic and fluorescent signals in standard IHC protocols. Use our complete systems or reagent packs.

In situ hybridization: amplify chromogenic and fluorescent signalsin ISH protocols. Eliminate the need for PCR (target amplification) when low or single copy genes are present. Use our complete systems or reagent packs.

ELISA: amplify signal in microplate-based assays, greatly increasing sensitivity or reducing the use of antibodies. Use our convenient ELAST ELISA Amplification System.

Protein microarrays: detect low abundance proteins in differential protein expression experiments. Use our TSA AUTOMATED Kits with our ProteinArray™ Workstation, a highly flexible, fully automated system for processing multiple protein microarrays.

Genomic microarrays: apply TSA to your cDNA differential gene expression studies using our MICROMAX™ TSA Labeling and Detection Kits.

Compatible with All Common Detection Methods

Chromogenic

BCIP/NBT (using alkaline phosphatase-conjugated secondary antibody or avidin)

DAB (using second application of HRP-conjugated secondary antibody or avidin)

ABC (avidin-biotinylated HRP complex)

PAP (peroxidase anti-peroxidase)

APAP (alkaline phosphatase anti-alkaline phosphatase)

Electron Microscopy (EM)

Gold conjugate

Immunogold detection systems

Visualize biotinyl tyramide directly

Fluorescence (Direct and Indirect)

239

Diagram of TSA direct fluorescence detection.

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Unparalleled sensitivity for challenging applications. Amplified signal reduces non-isotopic detection time.

Multi-target detection enables simultaneous visualizationof multiple targets.

Biotin-free formats eliminate background problems. Rivals IS-PCR results. Reduce the use of precious reagents.

Technology Showcase

Tyramide Signal Amplification (TSA)

Referenced by Leading Scientists WorldwideBecause TSA enables you to see previously undetectable levels of protein or nucleic acid, you gain invaluable new information and insight into your research. Hundreds of technical publications to date provide proof of the sensitivity, versatility and applicability of TSA technology to scientific research. For a listing, visit www.perkinelmer.com/TSA.

Put this outstanding technology to work for you — let PerkinElmer’s TSA amplify your research results!For a complete details about PerkinElmer’s Tyramide Signal Amplification (TSA) offerings, please see pp. 255–258 of this catalog or visit www.perkinelmer.com/TSA.

TSA is protected by US patents: 5,731,158, 5,583,001 and 5,196,306 and foreign equivalents.

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Radiolabeled Nucleotides

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Nucleic Acid Labeling & Detection

Customer Support e-mail: [email protected] Support e-mail: [email protected], [email protected]

Visit our website at www.perkinelmer.com/reagents

Radiolabeled NucleotidesPerkinElmer offers a complete line of 32P-, 33P- and 35S-labeled nucleotides, stocked in a broad variety of concentrations and specificactivities to meet your application needs.

All NEN Radionucleotides feature:

NENSure™ vial packaging for safety and convenience.

Generous pre-calibration.

Technical Data Sheet with safe handling, stability and storage information.

Always Fresh 32P Nucleotides: manufactured fresh everyMonday and Thursday.

• Shipped in a choice of blue plastic (BLU catalog number prefixes) or lead-lined plastic (NEG catalog number prefixes) packaging.

• Shipment in lead is required only for 32P quantities above 1.5 mCi.

• Help minimize hazardous waste: order lead-free packaging whenever feasible.

Always Fresh 33P Nucleotides: manufactured fresh everyFriday.

• Our 33P has very little 32P contamination: 0.3% versus1.2% or more from other manufacturers.

• Shipped in white plastic. Although shielding is neither necessary nor required, shipment in lead-lined plastic is available upon request.

35S Nucleotides: manufactured fresh the first Tuesday of every month.

• Shipped in yellow plastic. Although shielding is neither necessary nor required, shipment in lead-lined plastic is available upon request.

Our EasyTides® line of radionucleotides consists of stabilized,colored nucleotides prepared in a tricine-stabilized aqueoussolution that significantly extends the product shelf life(U.S. patent #4,390,517).

Shipped at ambient temperature: refrigerate upon receipt.

• Color coded by radionuclide:

• Emerald Green for 32P nucleotides

• Amber Gold for 33P nucleotides

• Sapphire Blue for 35S nucleotides

The NEN Radiochemicals Product Listing beginning on p. 46 of this catalog contains a complete listing of all NENRadionucleotides. In addition, at the beginning of the NENRadiochemicals section, you will find useful ReferenceGuides for each isotope containing summary informationabout our radionucleotide product range.

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Nucleic Acid Labeling & DetectionRadionucleotides Application Guide

Order by Phone: 1-800-762-4000 or 203-925-4600For country-specific ordering information, visit www.perkinelmer.com/lasoffices or see page 4

Order Online: www.perkinelmer.com/online242

Radionucleotides Application GuideUse this guide to help you select the optimal 32P, 33P or 35S nucleotide for your application.

Primary Application Compound Specific Activity Concentration Product Nos.

[Ci/mmol] [mCi/mL] [µM] EasyTides ShippedShipped ambient. frozen.

DNA Labeling (Random Primer dATP, [α-32P]- 800 10 12.5 BLU512A/NEG512A -

or Nick Translation) 3000 10 3.3 BLU512H/NEG512H BLU012H/NEG012H

6000 20 3.3 BLU512Z/NEG512Z BLU012Z/NEG012Z

DNA Sequencing (Klenow, T7, dCTP, [α-32P]- 800 10 12.5 BLU513A/NEG513A BLU013A

Thermophilic DNA Polymerases*) 3000 10 3.3 BLU513H/NEG513H BLU013H/NEG013H


dGTP, [α-32P]- 3000 10 3.3 BLU514H/NEG514H -


dTTP, [α-32P]- 800 10 12.5 BLU505A BLU005A

3000 10 3.3 BLU505H BLU005H/NEG005H

dATP, [α-33P]- 3000 10 3.3 NEG612H NEG312H

dCTP, [α-33P]- 3000 10 3.3 NEG613H NEG313H

dGTP, [α-33P]- 3000 10 3.3 NEG614H -

dTTP, [α-33P]- 3000 10 3.3 NEG605H -

dATPαS, [35S]- 500 10 20 - NEG034S

1250 12.5 10 NEG734H NEG034H

DNA 3' End Labeling 3'dATP, [α-32P]- 5000 10 2 - BLU026/NEG026

RNA Labeling ATP, [α-32P]- 800 10 12.5 - BLU003X

(SP6, T3, T7 RNA Polymerases) 3000 10 3.3 BLU503H BLU003H

CTP, [α-32P]- 800 10 12.5 NEG508X BLU008X/NEG008X

3000 10 3.3 BLU508H/NEG508H BLU008H

GTP, [α-32P]- 800 10 12.5 - BLU006X

3000 10 3.3 BLU506H/NEG506H BLU006H/NEG006H

UTP, [α-32P]- 800 10 12.5 BLU507X/NEG507X BLU007X/NEG007X

800 20 25 BLU507T/NEG507T -

800 40 50 NEG507C NEG007C



ATP, [α-33P]- 3000 10 3.3 NEG603H -

CTP, [α-33P]- 3000 10 3.3 NEG608H -

GTP, [α-33P]- 3000 10 3.3 NEG606H -

UTP, [α-33P]- 3000 10 3.3 NEG607H NEG307H

ATPαS, [35S]- 1250 12.5 10 - NEG033H

CTPαS, [35S]- 1250 12.5 10 - NEG064H

1250 70 56 - NEG064C

UTPαS, [35S]- 800 40 50 - NEG039C

1250 12.5 10 NEG739H NEG039H

RNA 3' End Labeling pCp, [γ-32P]- 3000 10 3.3 - BLU019A/NEG019A

Cycle Sequencing GTP, [γ-33P]- 3000 10 3.3 NEG604H -

Protein Kinase Assays ATP, [γ-33P]- 3000 10 3.3 NEG602H NEG302H

Protein Phosphorylation ATP, [γ-32P]- 10 2 200 - BLU002/NEG002


5' End Labeling 3000 10 3.3 BLU502A/NEG502A BLU002A/NEG002A

(Polynucleotide and Protein Kinases) 6000 10 1.7 BLU502Z/NEG502Z BLU002Z/NEG002Z

6000 150 0.04 - NEG035C

GTP, [γ-32P]- 6000 10 1.7 BLU504Z/NEG504Z BLU004Z/NEG004Z

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Nucleic Acid Labeling Systems/Hybridization Probes/Enzymes

243Customer Support e-mail: [email protected] Support e-mail: [email protected], [email protected]


Adenyl and Guanyl Cyclase Assays ATP, [α-32P]- 800 10 12.5 - BLU003X

3000 10 3.3 BLU503H BLU003H

GTP, [α-32P]- 800 10 12.5 - BLU006X

ADP Ribosylation of Proteins NAD, [32P]- 800 5 6.3 - BLU023X/NEG023X

G Protein/P2Y Receptor Studies GTPγS, [35S]- 1250 12.5 10 - NEG030H

Protein Thiophosphorylation ATPγS, [35S]- 25–100 12.5 125–500 - NEG027

1250 12.5 10 - NEG027H

* EasyTides should not be used with thermophilic polymerases. The high salt concentration of all the EasyTides dNTP’s interferes with this particular enzyme.

Primary Application Compound Specific Activity Concentration Product Nos.

[Ci/mmol] [mCi/mL] [µM] EasyTides ShippedShipped ambient. frozen.

Nucleic Acid Labeling SystemsThe Nucleic Acid Labeling Systems are used to generate radiolabeled probes for hybridization with complementary DNA or RNAsequences or for protein binding. Both the Oligonucleotide End Labeling Systems and the Random Primer Labeling Systems are widely used methods of labeling DNA probes.

PerkinElmer also offers some oligonucleotides for use as hybridization probes.

Product Description Product No.

Oligonucleotide 3' End Labeling System No isotope, 10 reactions NEP100001KT

Oligonucleotide 5' End Labeling System No isotope, 10 reactions NEP101001KT

Random Primer Extension Labeling System 20 reactions, 6 mer Primer NEP103X001KT

50 reactions, 6 mer Primer NEP103L001KT

Random Primer Plus Extension Labeling System 50 reactions, 14 mer Primer NEP112L001KT

Hybridization ProbesPerkinElmer also offers some oligonucleotides for use as hybridization probes.


Arginine-Vasopressin Oligonucleotide Hybridization Probe 100 pmol at 1 pmol/µL NEP505001EA

Enkephalin Oligonucleotide Hybridization Probe 100 pmol at 1 pmol/µL NEP502001EA

Neuropeptide Y Oligonucleotide Hybridization Probe 100 pmol at 1 pmol/µL NEP551001EA

Tumor Necrosis Factor-α Oligonucleotide Hybridization Probe Lyophilized, 5 µg at 100 mg/mL NLP053005UG

Enzymes


Glusulase (contains both β-Glucuronidase Glucuronidase >90,000 Units/mL NEE154001EA

and β-Glucuronide Sulfatase), 10 mL Glucuronide sulfatase >10,000 Units/mL

Glusulase (contains both β-Glucuronidase Glucuronidase >90,000 Units/mL NEE154B001EA

and β-Glucuronide Sulfatase), 100 mL Glucuronide sulfatase >10,000 Units/mL

Polynucleotide Kinase (T4) 2 x 100 Units NEE101001EA

5–10,000 Units/mL

500 Units NEE101A001EA

10–15,000 Units/mL

Terminal Transferase, 500 Units 10–16,000 Units/mL NEE114001EA

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Chemiluminescence & Chromogenic SubstratesNitro-Block II™ Luminescence EnhancerNitro-Block II™ Luminescence Enhancer provides enhanced signal intensity in blotting applications with dioxetane-based substratesolutions such as CDP- Star® on nitrocellulose or PVDF membranes.

Enhances signal on nitrocellulose membranes: obtain excellent results with CDP-Star ® in short exposures (10 to 45 minutes).

Reduces exposure times on PVDF membranes: up to ten-fold faster (as little as 15 seconds to 15 minutes for Western blots).

Available conveniently packaged with Western Lightning™ CDP-Star ® or DNA Thunder CDP-Star ®: ready for use with no extra reagent additions for easy conversion with 100% guaranteed performance.

Product Description Quantity Product No.

Nitro-Block II™ Luminescence Enhancer For 2,500 cm2 membrane 20 mL (20X concentrate) NEL615001EA

DNA Thunder CDP-Star ® with Nitro-Block II™ Enhancer For 2,000–12,500 cm2 of membrane 100 mL NEL618001KT

Ready to use.

Western Lightning CDP-Star ® with Nitro-Block II™ Enhancer For 2,000–12,500 cm2 of membrane 100 mL NEL616001KT

Ready to use.

DNA Thunder Chemiluminescence ReagentDNA Thunder™ taps the full power of high-sensitivity chemiluminescence detection and applies it to nucleic acid detection. It’s the first nucleic acid-specific chemiluminescence reagent, and the best choice for all your DNA and RNA imaging applications.

Achieve consistent, high-quality results: enables higher sensitivity and increased resolution with no change in protocol.

More for less: every bottle of reagent contains 20% more solution than most brands.

100% guarantee: reagent performance guaranteed for 12 months from ship date.


DNA Thunder Nucleic Acid Chemiluminescence Reagent For 2,500 cm2 of membrane 2 bottles (170 mL each) NEL201001EA

For 5,000 cm2 of membrane 4 bottles (170 mL each) NEL202001EA

DNA Thunder Nucleic Acid Chemiluminescence For 2,500 cm2 of membrane 2 bottles (170 mL each) NEL204001EA

Reagent Plus (Enhanced Luminol) For 5,000 cm2 of membrane 4 bottles (170 mL each) NEL205001EA

DNA Thunder CDP-Star ® Nucleic Acid For 5,000 cm2 of membrane 125 mL in 250 mL bottle NEL601001KT

Chemiluminescence Reagent

DNA Thunder CDP-Star ® with Nitro-Block II™ Enhancer For 2,000–12,500 cm2 of membrane 100 mL NEL618001KT

Ready to use.

Chromogenic Substrates

For colorimetric visualization of membrane-bound nucleic acids.


4CN Plus Chromogenic Substrate For up to 3,000 cm2 of membrane 2 bottles (15 mL of NEL300001EA

substrate, 75 mL of diluent)

BCIP/NBT Substrate Solution For up to 2,000 cm2 of membrane 2 x 250 mL NEL937001PK

(5-bromo-4-chloro-3-indolyl-phosphate

and nitroblue tetrazolium)

DAB Substrate Solution (3,3'-diaminobenzidine) 10 mL liquid concentrate NEL938001EA

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Protein Labeling & DetectionWestern Blotting Products/Chemiluminescence & Chromogenic Substrates



Protein Labeling & Detection

Western Blotting ProductsPerkinElmer offers a full line of products for Western blotting. Whether you prefer chemiluminescent or chromogenic detection,our products are the highest quality available, with the convenience of one-stop shopping and exceptional technical support.

Western Blotting Detection Options

Chemiluminescence Chromogenic Radiometric

Membrane PVDF or nitrocellulose PVDF PVDF or nitrocellulose PVDF or nitrocellulose

Reporter HRP conjugate AP conjugate HRP conjugate or AP conjugate 125I Protein A or 125I Protein G

Detection Substrate Western Lightning Western Lightning 4CN Plus, DAB or BCIP/NBT N/A

Chemiluminescence CDP-Star®

Reagent Plus Chemiluminescence

Reagent

Molecular Weight Markers Multicolored or Multicolored or Multicolored or biotinylated Multicolored or 14C-labeled

biotinylated biotinylated

Output Format KODAK™ Film KODAK™ Film Membrane KODAK™ Film

Chemiluminescence & Chromogenic Substrates

Western Lightning Chemiluminescence Reagent PlusAn enhanced luminol-based substrate for HRP-catalyzed detection of Western blots.


Western Lightning Western Blot Chemiluminescence For 1,000 cm2 of membrane 2 bottles (65 mL each) NEL103001EA

Reagent Plus (Enhanced Luminol) For 2,500 cm2 of membrane 2 bottles (170 mL each) NEL104001EA

For 5,000 cm2 of membrans 4 bottles (170 mL each) NEL105001EA

Western Lightning Chemiluminescence ReagentOur original enhanced luminol-based chemiluminescence reagent for rapid HRP-catalyzed protein detection.

Excellent reliability with the safety of nonisotopic protocols. Good sensitivity for many applications.


Western Lightning Western Blot Chemiluminescence For 1,000 cm2 of membrane 2 bottles (65 mL each) NEL100001EA

Reagent (Enhanced Luminol) For 2,500 cm2 of membrane 2 bottles (170 mL each) NEL101001EA

For 5,000 cm2 of membrane 4 bottles (170 mL each) NEL102001EA

High sensitivity.

Excellent signal-to-noise ratio using both PVDF and nitrocellulose membranes.

Excellent room temperature stability: stable unopened for at least 8 weeks.

Long shelf life: guaranteed for 12 months from ship date.

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Protein Labeling & DetectionChemiluminescence & Chromogenic Substrates/NitroGlo® Nitrosylation Kit



Western Lightning CDP-Star ® Chemiluminescence ReagentFor alkaline phosphatase-catalyzed protein detection.


Western Lightning CDP-Star ® Chemiluminescence Reagent For 5,000 cm2 of membrane 1 bottle (125 mL) NEL602001KT

Ready to use.

Western Lightning CDP-Star ® with Nitro-Block II™ Enhancer For 2,000–12,500 cm2 of membrane 100 mL NEL616001KT

Ready to use.

Chromogenic Substrates

For colorimetric visualization of membrane-bound proteins.


4CN Plus Chromogenic Substrate (4-chloro-1-naphthol) For up to 3,000 cm2 of membrane 2 bottles (15 mL of substrate, NEL300001EA

75 mL of diluent)

BCIP/NBT Substrate Solution For up to 2,000 cm2 of membrane 2 x 250 mL NEL937001PK

(5-bromo-4-chloro-3-indolyl-phosphate

and nitroblue tetrazolium)

DAB Substrate Solution (3,3'-diaminobenzidine) 10 mL liquid concentrate NEL938001EA

NitroGlo Nitrosylation KitFor detecting and quantifying nitrosylated proteins and proteins susceptible to nitrosylation — simply and accurately.

Total convenience in a single, step-by-step kit: everything you need to detect and fingerprint S-nitrosylated proteins in tissue samples,protein mixtures or purified proteins.

Visualize and quantify the resulting protein bands easily: use our Western Lightning Chemiluminescence Reagents and any commercial imaging system.


NitroGlo® Nitrosylation Kit 50 reactions/kit NOS101001KT

Fastest kinetics for short film exposures.

Signal develops in only 30 minutes: maximum signal strength in just two hours.

Continuous strong signal for 24 hours.

Substrate is well suited for re-exposure.

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Protein Labeling & DetectionMolecular Weight Markers



Protein Molecular Weight MarkersThese markers provide standards for molecular weight determinations in Western blotting applications.

Multicolor Protein Molecular Weight Markers

Provide standards for molecular weight determinations in Western blotting applications.

Provide a visual monitor of migration during electrophoresis and transfer efficiency for isotopic and nonisotopic applications.

Choice of high, low and wide molecular weight ranges.

Protein Mix of Multicolor Molecular Weight Markers

Protein Molecular Weight (Daltons) Color Range

High Wide Low

Myosin, rabbit muscle 205,000 Blue X X

β-Galactosidase, E. coli 116,000 Turquoise X X

Albumin, bovine serum 66,000 Pink X X

Ovalbumin, chicken egg white 45,000 Yellow X X X

Carbonic anhydrase, bovine erythrocyte 29,000 Orange X X X

Trypsin inhibitor, soybean 20,100 Green X X

α-Lactalbumin, bovine milk 14,200 Purple X X

Aprotinin, bovine lung 6,500 Blue X X


Multicolored Protein Markers, wide range 500 µL NEL311001EA

Multicolored Protein Markers, high range 500 µL NEL312001EA

Multicolored Protein Markers, low range 500 µL NEL313001EA

Biotinylated Protein Molecular Weight Markers

For nonisotopic Western blotting applications.

Result in a ladder of 6 equal intensity bands ranging from 12,300 to 97,400 Daltons.

Excellent for determining precise molecular weight measurements.


Biotinylated Protein Molecular Weight Markers 1 mg/mL in 50 µL in 50% glycerol NEL310001EA

Protein Mix of Biotinylated Molecular Weight Markers

Protein Molecular Weight (Daltons)

Phosphorylase B, rabbit muscle 97,400

Albumin, bovine serum 69,000

Ovalbumin, chicken egg white 46,000


Carbonic anhydrase, bovine erythrocyte 30,000

Trypsin inhibitor, soybean 20,100

Cytochrome C, horse heart 12,300

Wide range High range Low range

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Protein Labeling & DetectionMolecular Weight Markers/Recombinant 125I-Protein A and 125I-Protein G



14C Methlyated Molecular Weight Markers

For use in radiometric applications.

The electrophoretic mobility of methylated molecular weight markers is identical to the unmodified individual protein.


14C Methylated Molecular Weight Markers 1 µCi (37 kBq) NEC811001UC

5 µCi (185 kBq) NEC811005UC

Protein Mix of 14C Methylated Molecular Weight Markers


Phosphorylase B 97,400

Albumin, bovine serum 69,000

Gamma globulin (subunit 1) 53,000


Ovalbumin 46,000

Gamma globulin (subunit 2) 22,500

Cytochrome C 12,300

Recombinant 125I-Protein A and 125I-Protein GProtein A and Protein G are both suitable for direct autoradiographic detection of membrane-bound antibody in Western blotting applications.

Product Package Size Product No.

Protein A, [125I]-, (human, recombinant) 10 µCi (370 kBq) NEX146010UC

70 –100 µCi (2.59–3.7 MBq)/µg 25 µCi (925 kBq) NEX146025UC

Packaged in phosphate buffer (pH 4.0) containing 35% ethanol.

Protein A, [125I]-, (human, recombinant) 100 µCi (3.7 MBq) NEX146L100UC

2–10 µCi (74–370 kBq)/µg 250 µCi (9.25 MBq) NEX146L250UC

Packaged in phosphate buffer (pH 4.0) containing 35% ethanol.

Protein G, [125I]-, Bolton-Hunter labeled, (recombinant) 10 µCi (370 kBq) NEX237010UC

15 – 25 µCi (555–925 MBq)/µg 50 µCi (1.85 MBq) NEX237050UC

Packaged in sodium phosphate-buffered saline (pH 5.2) 100 µCi (3.7 MBq) NEX237100UC

containing glycine and BSA.

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Blotting & AutoradiographyConjugated Secondary Antibodies/Hybridization Transfer Membranes



Conjugated Secondary AntibodiesOur conjugated secondary antibodies provide high sensitivity and specificity in chromogenic or chemiluminescent applications.

Affinity purified from goat serum immunized with purified IgG.

Choose from alkaline phosphatase, horseradish peroxidase or biotin conjugates.

Antibody Label Quantity Concentration Product No.

Anti-human IgG (goat)* AP 1 mg 1 mg/mL NEF801001EA

Anti-human IgG (goat)* HRP 1 mg 1 mg/mL NEF802001EA

Anti-human IgG (goat) Biotin 0.5 mg Lyophilized NEF803001EA

Anti-mouse IgG (goat) AP 1 mg 1 mg/mL NEF821001EA

Anti-mouse IgG (goat) HRP 1 mg 1 mg/mL NEF822001EA

Anti-mouse IgG (goat) Biotin 0.5 mg Lyophilized NEF823001EA

Anti-rabbit IgG (goat) AP 1 mg 1 mg/mL NEF811001EA

Anti-rabbit IgG (goat) HRP 1 mg 1 mg/mL NEF812001EA

Anti-rabbit IgG (goat) Biotin 0.5 mg Lyophilized NEF813001EA

* Bovine serum albumin is added as a stabilizer.

Blotting & Autoradiography

Hybridization Transfer MembranesTo assure quality detection, PerkinElmer provides a variety of high performance membranes for your applications. These include our popular GeneScreen™, GeneScreen Plus®, GeneScreen Plus NR®, and Colony/Plaque Screen™ nylon membranes, and our PolyScreen®

PVDF membranes. Additionally, we are an authorized worldwide dealer of Schleicher & Schuell Protran® nitrocellulose membranes.

Nylon Transfer MembranesPerkinElmer’s GeneScreen, GeneScreen Plus, GeneScreenPlus NR, and Colony/Plaque Screen nylon membranes arepure white and perfectly smooth, with uniform pore sizes.They are available in positively charged and neutral membranes, in sheets, rolls, discs or custom cuts.

GeneScreen and GeneScreen PlusGeneScreen and GeneScreen Plus are excellent alternativesto nitrocellulose or other nylon membranes.

• Perfect for Northern and Southern blotting applications.

• Rugged, flexible, and easy to handle: no cracking or tearing.

• Both sides are the same: no more guessing which side is “up”.

• Wetting is instant and complete: every site is evenly prepared.

• Won’t shrink, distort, or curl: be confident in your molecular weight determinations.

• Non-flammable: no risk of fire during baking.

GeneScreen Plus Charged Nylon Membranes• Best choice for radiometric techniques.

• Highest sensitivity for radiometric detection in nucleicacid hybridization.

• High affinity for RNA: 500% greater retention than nitrocellulose.

• Binds DNA of all sizes, from oligonucleotides to fragments >23 kb.

• Positive charge creates a very strong bond: no need to fix nucleic acids to the membrane.

• High bond strength increases the number of re-probes possible.

• Bond strength and uniform pore size (0.45 µm) improvesresolution by minimizing diffusion during transfer.

GeneScreen & GeneScreen Plus NR Neutral Nylon Membranes• Best choice for chemiluminescent and chromogenic

techniques: provides low background.

• Cross linking occurs quickly: only two minutes of UV irradiation required.

• GeneScreen is excellent for DNA nylon arrays.

• GeneScreen Plus NR is ideal for chemiluminescencedetection of Northern and Southern blots, providing lower background with no change in protocol.

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yBlotting & AutoradiographyGeneScreen/Colony/Plaque Screen Discs & Membranes



Product Quantity & Size Product No.

GeneScreen Plus Charged Hybridization 1 (20 cm x 3 m) roll NEF1017001PK

Transfer Membranes 1 (30 cm x 3 m) roll NEF988001PK

5 (30 x 45 cm) sheets NEF976001PK



20 (8.5 x 12.4 cm) sheets NEF994001PK

GeneScreen Plus NR Hybridization Transfer Membrane 1 (30 cm x 3 m) roll NEF1016001PK

GeneScreen Neutral Hybridization Transfer Membranes 1 (20 cm x 3 m) roll NEF1018001PK

1 (30 cm x 3 m) roll NEF983001PK



Colony/Plaque Screen Nylon Membrane DiscsOur Colony/Plaque Screen nylon membrane discs are precut for use in plaque hybridization protocols and bacterial colony screenings.

Withstand rough treatment: won’t shrink, crack or tear.

Convenient orientation holes make alignment precise.

Nylon lift tags mean removal is easy and tear-resistant.

Lifts are complete: the discs fit right to the edge of the plate.

Colony/Plaque Screen Hybridization Transfer Membrane for Nonisotopic Detection

Neutral nylon membrane.

Great for chemiluminescent or chromogenic techniques.

Colony/Plaque Screen Hybridization Transfer Membrane

Charged nylon membrane.

Great for radiometric techniques.


Colony/Plaque Screen Hybridization Transfer Membrane 50 (82 mm diameter) discs interleaved with NEF1010001PK

for nonisotopic detection plastic sheets (0.45 µm pore size)

Colony/Plaque Screen Hybridization Transfer Membrane 50 (82 mm diameter) discs with lift tabs and orientation holes NEF978X001PK

50 (137 mm diameter) discs with lift tabs and orientation holes NEF978Y001PK

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Blotting & AutoradiographyPolyScreen PVDF Transfer Membranes/Nitrocellulose Transfer Membranes



PolyScreen PVDF Transfer MembranesA microporous polyvinylidene difluoride membrane, PolyScreen is an effective alternative to nitrocellulose membranes for protein transfers.

Sensitive: detects low levels of protein and decreases exposure times.

Easy to use: adapts to most nitrocellulose membrane protocols with the addition of a pre-wetting step.

Durable: stronger than nitrocellulose, it won’t tear, crack or shrink, so there are no rips or crackles on your autorads and your molecular weight markers are not distorted.

Reprobable: retains more protein than nitrocellulose, even during the most stringent washes.

Flexible: for use with standard blocking reagents (nonfat dry milk, casein, non-ionic surfactants or BSA) and all common immunostains,chromogenic and chemiluminescent substrates.

Wettable: entire membrane is prepared consistently, so transfers are more efficient.

Archivable: won’t shatter when dry, or discolor over time.

Safe: nonflammable, requires no special handling, storage or shipping precautions.

Versatile: can be used for protein staining (compatible with commonly used stains and withstands high solvent concentrations in rapid destaining), immunoblotting, protein sequencing and nitrosylation studies.

PolyScreen is ideal for use with PerkinElmer’s Western Lightning Chemiluminescence Reagents using KODAK™ BioMax®

autoradiography films.


PolyScreen PVDF Hybridization Transfer Membrane 1 (26.5 cm x 3.75 m) roll NEF1002001PK


PolyScreen PVDF Hybridization Transfer Membrane for “Mini Gels” 50 (7 x 8.4 cm) sheets NEF1003001PK

Nitrocellulose Transfer MembranesPerkinElmer Life and Analytical Sciences is an authorized, worldwide dealer of Schleicher & Schuell membranes. Choose from a fullselection of superior S & S Protran® nitrocellulose membranes to suit your needs.

Pure: 100% nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.

Low background: superior surface properties guarantee superior signal to noise ratios, without stringent washing conditions.

High retention of small proteins: 0.2 µm size membrane ensures high retention of small samples below 20 kD by reducing “blowthrough”. [0.45 µm pore size membrane (NBA085 series) is ideal for larger molecular weight samples.]

No methanol pre-wetting step: prior to transfer, the membrane is simply wet in water, and then in the transfer buffer.No other pre-treatment steps are necessary.

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yBlotting & AutoradiographyProtran® Membranes/KODAK™ Autoradiography Films, Screens & Accessories



Protran® Pure Nitrocellulose Membranes


Protran® Nitrocellulose (0.2 µm pore size) 1 (15 x 15 cm) sample sheet NBA083S001EA

1 (30 cm x 3 m) roll NBA083C001EA

5 (15 x 15 cm) sheets NBA083D001EA

5 (20 x 20 cm) sheets NBA083E001EA

5 (25 x 25 cm) sheets NBA083F001EA

5 (33 x 56 cm) sheets NBA083G001EA

Protran® Nitrocellulose (0.45 µm pore size) 1 (15 x 15 cm) sample sheet NBA085S001EA

1 (15 cm x 3 m) roll NBA085A001EA

1 (20 cm x 3 m) roll NBA085B001EA

1 (30 cm x 3 m) roll NBA085C001EA

5 (15 x 15 cm) sheets NBA085D001EA

5 (20 x 20 cm) sheets NBA085E001EA

5 (25 x 25 cm) sheets NBA085F001EA

5 (33 x 56 cm) sheets NBA085G001EA

Protran® Unsupported Nitrocellulose Circles (0.45 µm pore size) 50 circles, 25 mm NBA085H001EA

50 circles, 82 mm NBA085I001EA

50 circles, 132 mm NBA085J001EA

50 circles, 137 mm NBA085K001EA

KODAK™ Autoradiography Films, Screens & AccessoriesPerkinElmer is an authorized dealer of KODAK™ Molecular Imaging Systems. Choose from a full selectionof KODAK™ film/screen combinations to suit your needs. KODAK™ BioMax® films and screens are optimized to provide the resolution, clarity, sensitivity and speed you require for your applications.

BioMax® MR Film

Ideal choice for detection of sequencing gels.

Single-emulsion format increases resolution and background clarity, for easier reading and more accurate results.

Sensitivity two to four times that of other films, resulting in improved detection of low-intensity bands.

Product/Size Quantity/Packaging Product No.

BioMax® MR-1 Autoradiography Film, 13 x 18 cm (5 x 7 in.) 50 sheets, non-interleaved packaging 8941114001EA

BioMax® MR-1 Autoradiography Film, 18 x 24 cm (7 x 9.5 in.) 50 sheets, non-interleaved packaging 8736936001EA


BioMax® MR-1 Autoradiography Film, 20.3 x 25.4 cm (8 x 10 in.) 50 sheets, non-interleaved packaging 8701302001EA

BioMax® MR-1 Autoradiography Film, 24 x 30 cm (9.5 x 11.8 in.) 50 sheets, non-interleaved packaging 8912560001EA

BioMax® MR-1 Autoradiography Film, 30 x 40 cm (11.8 x 15.8 in.) 50 sheets, non-interleaved packaging 8929655001EA



BioMax® MR-2 Autoradiography Film, 13 x 18 cm (5 x 7 in.) 50-sheet ReadyPack; each sheet individually wrapped 8572786001EA

BioMax® MR-2 Autoradiography Film, 20.3 x 25.4 cm (8 x 10 in.) 50-sheet ReadyPack; each sheet individually wrapped 8952855001EA

BioMax® MR-2 Autoradiography Film, 35 x 43 cm (14 x 17 in.) 50-sheet ReadyPack; each sheet individually wrapped 8567232001EA

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Blotting & AutoradiographyBioMax® MS Film/BioMax® Light Film/BioMax® XAR Film



BioMax® MS Film

High performance all-purpose, maximum sensitivity autoradiography film, double-side coated with emulsion.

Ideal choice for detection of Northern, Southern and dot blots, especially when detecting low intensity samples or when needing to reduce exposure time.

Use with BioMax® TranScreen HE intensifying screen for four to eight times greater sensitivity detecting 32P than any other film/screen combination available.

BioMax® MS film and BioMax® MS intensifying screen used together deliver up to nine times greater sensitivity than some other film/screen combinations allowing significant reductions in exposure times.


BioMax® MS-1 Autoradiography Film, 13 x 18 cm (5 x 7 in.) 50 sheets, non-interleaved packaging 1111681001EA

BioMax® MS-1 Autoradiography Film, 18 x 24 cm (7 x 9.5 in.) 50 sheets, non-interleaved packaging 8222648001EA

BioMax® MS-1 Autoradiography Film, 20.3 x 25.4 cm (8 x 10 in.) 50 sheets, non-interleaved packaging 8294985001EA

BioMax® MS-1 Autoradiography Film, 24 x 30 cm (9.5 x 11.8 in.) 50 sheets, non-interleaved packaging 8326886001EA

BioMax® MS-1 Autoradiography Film, 30 x 40 cm (11.8 x 15.8 in.) 50 sheets, non-interleaved packaging 8220774001EA

BioMax® MS-1 Autoradiography Film, 35 x 43 cm (14 x 17 in.) 50 sheets, non-interleaved packaging 1435726001EA

BioMax® MS-2 Autoradiography Film, 20.3 x 25.4 cm (8 x 10 in.) 50-sheet ReadyPack; each sheet individually wrapped 8377616001EA

BioMax® MS-2 Autoradiography Film, 35 x 43 cm (14 x 17 in.) 50-sheet ReadyPack; each sheet individually wrapped 1764505001EA

BioMax® Light Film

Best choice for detection of chemiluminescence labels.

Maximum clarity and sensitivity.

Highest signal-to-noise ratio of any scientific film.


BioMax® Light-1 Autoradiography Film, 13 x 18 cm (5 x 7 in.) 50 sheets, non-interleaved packaging 8689358001EA

BioMax® Light-1 Autoradiography Film, 18 x 24 cm (7 x 9.5 in.) 50 sheets, non-interleaved packaging 8194540001EA

BioMax® Light-1 Autoradiography Film, 20.3 x 25.4 cm (8 x 10 in.) 50 sheets, non-interleaved packaging 1788207001EA

BioMax® Light-2 Autoradiography Film, 13 x 18 cm (5 x 7 in.) 50-sheet ReadyPack; each sheet individually wrapped 1917012001EA

BioMax® Light-2 Autoradiography Film, 20.3 x 25.4 cm (8 x 10 in.) 50-sheet ReadyPack; each sheet individually wrapped 8761520001EA

BioMax® XAR Film

Industry standard general purpose film for use with all commonly used isotopes and chemiluminescence labels.

Coated with emulsion on both sides of a clear base.

High sensitivity for direct autoradiography and for exposures with BioMax® MS or TranScreen intensifying screens.


BioMax® XAR Film, 13 x 18 cm (5 x 7 in.) 50 sheets, non-interleaved packaging 1651496001EA

BioMax® XAR Film, 18 x 24 cm (7 x 9.5 in.) 50 sheets, non-interleaved packaging 8532665001EA

BioMax® XAR Film, 20.3 x 25.4 cm (8 x 10 in.) 50 sheets, non-interleaved packaging 1651454001EA

BioMax® XAR Film, 24 x 30 cm (9.5 x 11.8 in.) 50 sheets, non-interleaved packaging 1501451001EA

BioMax® XAR Film, 30 x 40 cm (11.8 x 15.8 in.) 50 sheets, non-interleaved packaging 1506955001EA

BioMax® XAR Film, 35 x 43 cm (14 x 17 in.) 50 sheets, non-interleaved packaging 1651512001EA

BioMax® XAR Film Ready Pack, 20.3 x 25.4 cm (8 x 10 in.) 50-sheet ReadyPack; each sheet individually wrapped 1651579001EA

BioMax® XAR Film Ready Pack, 35 x 43 cm (14 x 17 in.) 50-sheet ReadyPack; each sheet individually wrapped 1651678001EA

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yBlotting & AutoradiographyX-OMAT Blue Film/BioMax® TranScreen HE & LE Screens & Accessories



X-OMAT™ Blue FilmA PerkinElmer exclusive!

Fine-grained, low-fog blue film that provides excellent sensitivity, sharp resolution, and high contrast images.

Economical choice for chemiluminescence detection and all commonly used isotopes.

Most sensitive blue film for luminol-based chemiluminescence and the fastest for dioxetane-based chemiluminescence.

Use with BioMax® MS screen for excellent results detecting 32P-labeled probes.


X-OMAT Blue (XB) Film, 13 x 18 cm (5 x 7 in.) 100 sheets, non-interleaved packaging NEF586001EA

X-OMAT Blue (XB) Film, 18 x 24 cm (7 x 9.5 in.) 100 sheets, non-interleaved packaging NEF585001EA

X-OMAT Blue (XB) Film, 20.3 x 25.4 cm (8 x 10 in.) 100 sheets, non-interleaved packaging NEF596001EA

X-OMAT Blue (XB) Film, 35 x 43 cm (14 x 17 in.) 100 sheets, non-interleaved packaging NEF595001EA

BioMax® TranScreen HE and LE Intensifying Screens

Five times faster results than conventional intensifying screens.

No autoradiography enhancers required.*

TranScreen LE: the first intensifying screens for low- and moderate-energy isotopes (e.g., 3H, 35S, 14C, 33P, 45Ca and 59Fe).

TranScreen HE: designed for use with high energy (HE) beta emitters such as 32P and 125I, providing high sensitivity and high resolution for these penetrating isotopes. Produces publication-quality images.

* For other autoradiography techniques, PerkinElmer offers a variety of enhancers to meet your individual needs. (See Autoradiography Enhancers, p. 255).


BioMax® TranScreen HE, 20.3 x 25.4 cm (8 x 10 in.) One screen 8563959001EA

BioMax® TranScreen HE, 35 x 43 cm (14 x 17 in.) One screen 8811457001EA

BioMax® TranScreen HE, with cassette, 20.3 x 25.4 cm (8 x 10 in.) One screen plus one cassette 8356800001EA

BioMax® TranScreen LE, 20.3 x 25.4 cm (8 x 10 in.) One screen 1622034001EA

BioMax® TranScreen LE, with cassette, 20.3 x 25.4 cm (8 x 10 in.) One screen plus one cassette 1030667001EA

AccessoriesPerkinElmer carries a range of KODAK™ accessories for autoradiography, including film cassettes, intensifying screens and film processing hangers.


BioMax® MS Intensifying Screen, 18 x 24 cm (7 x 9.5 in.) One screen 8757270001EA

BioMax® MS Intensifying Screen, 20.3 x 25.4 cm (8 x 10 in.) One screen 8518706001EA

BioMax® MS Intensifying Screen, 30 x 40 cm (11.8 x 15.8 in.) One screen 8770331001EA

BioMax® MS Intensifying Screen, 35 x 43 cm (14 x 17 in.) One screen 8981706001EA

BioMax® Cassette, with one BioMax® MS Screen, 20.3 x 25.4 cm (8 x 10 in.) One cassette plus one screen 8750499001EA

BioMax® Cassette, with one BioMax® MS Screen, 35 x 43 cm (14 x 17 in.) One cassette plus one screen 8578825001EA

BioMax® Cassette, with one BioMax® TranScreen HE, 20.3 x 25.4 cm (8 x 10 in.) One cassette plus one screen 8356800001EA

BioMax® Cassette, with one BioMax® TranScreen LE, 20.3 x 25.4 cm (8 x 10 in.) One cassette plus one screen 1030667001EA

BioMax® Cassette, without screen, 20.3 x 25.4 cm (8 x 10 in.) One cassette 8209140001EA

BioMax® Cassette, without screen, 35 x 43 cm (14 x 17 in.) One cassette 8197600001EA

Film Processing Hanger, 13 x 18 cm (5 x 7 in.) One hanger 1502723001EA

Film Processing Hanger, 20.3 x 25.4 cm (8 x 10 in.) One hanger 1502764001EA

All KODAK™ products are shipped and stored at ambient temperature.

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Autoradiography Enhancers/TSA Systems



Autoradiography EnhancersThe use of KODAK™ TranScreen LE or TranScreen HE intensifying screen eliminates the need for autoradiography enhancers. For other autoradiography techniques, PerkinElmer offers a variety of enhancers to meet your particular needs.

Product Size Description Product No.

EN3HANCE® Autoradiography Enhancer 1 L Use on gels for best band resolution; good sensitivity 6NE9701

on acrylamide; best sensitivity with agarose.

EN3HANCE Spray Surface Autoradiography Enhancer 2 oz. (59.1 mL) Use on solid supports such as paper, membranes 6NE970C

spray can and TLC.

ENLIGHTNING™ Rapid Autoradiography Enhancer 1 L Safer alternative to 6NE9701. Use on gels. 6NE9741

Immunohistochemistry & In Situ Hybridization

Tyramide Signal Amplification (TSA) SystemsPerkinElmer’s patented Tyramide Signal Amplification(TSA) technology, extensively referenced in scientific literature, is widely recognized as a valuable tool for amplifying both chromogenic and fluorescent signalsobtained with standard immunohistochemistry (IHC) and in situ hybridization (ISH) protocols.

Our TSA systems are formatted to enable every lab to benefit from the sensitivity enhancements made possible by applying this innovative technology.

Benefits in Use• Provides a significant increase in sensitivity, without

loss of resolution or increase in background.

• Eliminates need for IS-PCR (target amplification) sometimes used prior to ISH assays when low or single copy genes are present.

• Utilizes up to 1,000-fold less primary antibody and main-tains detection levels, when used in IHC assays, ofteneliminating the need for antigen retrieval procedures.

• Conserves limited or expensive probes when added to ISH protocols.

• Enables visualization of previously undetectable levels of protein or nucleic acid, possibly leading to newresearch insights or discoveries.

Choosing a TSA SystemChoose a system that best suits your research needs:

• Select chromogenic or fluorescence detection based on the equipment available to your laboratory and your need for a permanent record.

• Eliminate biotin interference: consider the level ofendogenous biotin in your specimens and your desire to eliminate biotin-quenching procedures.

• Choose from several fluorophores based on your preferredfluors and your interest in performing multi-target detection.

• Determine the degree of amplification you need, takinginto account the abundance of the protein or gene in yourspecimen and your need to conserve antibody or probe.

TSA is compatible with all common chromogenic and fluo-rescence detection methods, and is easily integrated into anyprotocol after an initial addition of horseradish peroxidase(HRP). Based on your experience and sensitivity require-ments, one of three formats will best fit your needs:

Complete Systems

• TSA Systems: complete tyramide signal amplificationkits for IHC or ISH applications.

• TSA Plus Systems: tyramide signal amplification kits thatprovide 10 to 20 times the sensitivity enhancement of regular TSA systems. Available in a biotin-free DNP alternative for chromogenic detection.

Reagent Packs

• TSA Reagents: stand-alone amplification reagents thatprovide researchers experienced with TSA the flexibilityto develop their own protocols.

Complete TSA SystemsAll TSA systems include tyramide labeled with either a haptenor a fluorophore, amplification diluent and a comprehensiveinstruction manual. Additional antibodies, conjugates such as streptavadin-HRP, and chromogens may be required foryour optimal protocol and may be purchased separately.

TSA Plus Fluorescence Systems provide very high sensitivityin ISH compared to traditional methods. While developedspecifically for ISH applications, these systems can also beused with IHC applications.

TSA Plus DNP systems eliminates the need for biotinquenching procedures, improving the sensitivity of detec-tion. These are specifically tested and optimized for use with digoxigenin probe labeling technology.

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Immunohistochemistry & In Situ HybridizationTSA Systems



TSA Biotin Systems

Designed for chromogenic detection in ISH and IHC applications.

Use streptavidin-conjugated chromogens or fluorophores for visualization.

TSA Biotin Systems

Product No. of Slides Product No.

TSA Biotin System 50–150 NEL700A001KT

200–600 NEL700001KT

TSA Plus DNP Systems

Designed for biotin-free chromogenic detection in ISH and IHC applications.

Use to reduce background associated with endogenous biotin.

Simple protocol eliminates biotin-quenching procedures.

When performing ISH procedures, any hapten-labeled probe can be used.

The ISH protocol included is specifically optimized for use with popular DIG-labeled probes.

Biotin-Free Kits for Chromogenic Detection


TSA Plus DNP (AP) System 25–75 NEL746B001KT

50–150 NEL746A001KT

TSA Plus DNP (HRP) System 25–75 NEL747B001KT

50–150 NEL747A001KT

IHC of EBV antigen in Hodgkin’s Lymphoma of mixed cellularity. Comparisonshows (A) Anti-EBV dilution 1:25, without TSA enhancement, (B) Anti-EBVdilution 1:25,000, enhanced by TSA. (Courtesy of R. Von Wasielewski and S. Gignac, Pathologisches Institut de Medizinischen Hochscule,Hannover, Germany.)

TSA Systems conserve precious antibody or probe

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TSA Plus DNP brings enhanced sensitivity to existing DIG labeling protocols

Detection of p53 mRNA in Lung Tissue. Digoxigenin-labeled p53 RNAprobes were hybridized to paraffin-embedded lung tissue. Comparisonshows (A) standard digoxigenin detection with alkaline phosphatase/BCIP-NBT (60 minute substrate incubation), and (B) TSA Plus with alkaline phosphatase/BCIP-NBT (15 minute substrate incubation).

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TSA Systems/Multi-Target Fluorescence Detection



TSA and TSA Plus Direct Fluorescence Systems

Designed for direct fluorescence detection in ISH or IHC procedures.

Choose from five different fluorophores: fluorescein tetramethylrhodamine (TMR), coumarin, Cyanine 3 or Cyanine 5,and either original TSA or TSA Plus systems.

TSA Kits for Fluorescence Detection


TSA Fluorescein System 50–150 NEL701A001KT

100–300 NEL701001KT

TSA TMR System 100–300 NEL702001001KT

TSA Coumarin System 100–300 NEL703001KT

TSA Cyanine 3 System 50–150 NEL704A001KT

TSA Cyanine 5 System* 50–150 NEL705A001KT

TSA Plus Kits for Fluorescence Detection


TSA Plus Fluorescein System 50–150 NEL741001KT

250–750 NEL741B001KT

TSA Plus TMR System 50–150 NEL742001KT

250–750 NEL742B001KT

TSA Plus Cyanine 3 System 50–150 NEL744001KT

250–750 NEL744B001KT

TSA Plus Cyanine 5 System* 50–150 NEL745001KT

250–750 NEL745B001KT

* Cyanine 5 tyramides cannot be visualized with most digital cameras.

Multi-Target Fluorescence DetectionTSA Plus Fluorescence Systems facilitate multi-target detection procedures. Choose from four different two-fluor combinations,as well as our four-fluor Palette System for evaluation.

TSA Plus Multi-Fluor Combination Kits


TSA Plus Cyanine 3/Cyanine 5 System* 50–150 NEL752001KT

TSA Plus Cyanine 3/Fluorescein System 50–150 NEL753001KT

TSA Plus Cyanine 5/Fluorescein System* 50–150 NEL754001KT

TSA Plus Fluorescein/TMR System 50–150 NEL756001KT

TSA Plus Evaluation Kit


TSA Plus Fluorescence Palette System 10–35 NEL760001KT

(contains one each of Fluorescein, TMR, Cyanine 3 and Cyanine 5)*


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Immunohistochemistry & In Situ HybridizationTSA Reagent Packs/Complementary Products for Use with TSA



TSA Reagent PacksTSA Reagent Packs are stand-alone tyramide (SAT) kits for signal amplification that provide flexibility to researchers for TSA protocol development.

Researchers must supply their own blocking buffer, HRP conjugate and other reagents as needed.

If you have never used TSA Signal Amplification reagents, we strongly suggest purchasing one of our full TSA Systems,which include a set of quality controlled reagents that have been optimized to work together.

TSA SAT Kits for Signal Amplification


TSA Biotin Tyramide Reagent Pack 200– 600 SAT700001EA

1,000–3,000 SAT700B001EA

TSA Fluorescein Tyramide Reagent Pack 100– 300 SAT701001EA

500–1,500 SAT701B001EA

TSA TMR Tyramide Reagent Pack 100– 300 SAT702001EA

TSA Cyanine 3 Tyramide Reagent Pack 50–150 SAT704A001EA

250–750 SAT704B001EA

TSA Cyanine 5 Tyramide Reagent Pack* 50–150 SAT705A001EA


Complementary Products for Use with TSAThese high quality conjugates, chromogens and secondary antibodies can be used with TSA Assay Systems and Reagent Packs.

Chromogens & Conjugates

Product Product No.

BCIP/NBT (AP substrate) NEL937001PK

DAB (HRP substrate) NEL938001EA

Antifluorescein-AP NEF709001PK

Antifluorescein-HRP NEF710001EA

Streptavidin Fluorescein NEL720001EA

Product Product No.

Streptavidin Texas Red® NEL721001EA

Streptavidin Coumarin NEL722001EA

Streptavidin-HRP NEL750001EA

Streptavidin-AP NEL751001EA

Secondary Antibodies

Antibody Label Size Concentration Product No.

Anti-human IgG (goat)* AP 1 mL 1 mg/mL NEF801001EA

Anti-human IgG (goat)* HRP 1 mL 1 mg/mL NEF802001EA

Anti-human IgG (goat) Biotin 0.5 mg Lyophilized NEF803001EA

Anti-mouse IgG (goat) AP 1 mL 1 mg/mL NEF821001EA

Anti-mouse IgG (goat) HRP 1 mL 1 mg/mL NEF822001EA

Anti-mouse IgG (goat) Biotin 0.5 mg Lyophilized NEF823001EA

Anti-rabbit IgG (goat) AP 1 mL 1 mg/mL NEF811001EA

Anti-rabbit IgG (goat) HRP 1 mL 1 mg/mL NEF812001EA

Anti-rabbit IgG (goat) Biotin 0.5 mg Lyophilized NEF813001EA

* Bovine serum albumin added as a stabilizer.

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Nucleotide Analogs & cDNA Microarray AnalysisDirect Fluorescence Detection Labeling Reagents: Dideoxynucleotide Analogs



PerkinElmer supports all genomic applications, from microarrays and single nucleotide polymorphisms (SNPs), to nucleic acid blotting and in situ hybridization. All four labeled base modifications are available in riboNTP, deoxyNTP and dideoxyNTP,so you can chose the best combination of dye/hapten nucleotides for your application.

PerkinElmer will custom synthesize and purify any labeled nucleotide for your research. You provide the amine-reactive label(NHS, isothiocyanate, sulfonyl chloride, etc.), and we will attach it to any of a wide variety of amino-modified nucleotide 5'-triphosphates. PerkinElmer’s proprietary purification system provides analogs of the highest purity available. In many cases, the analog can be provided isomerically pure.

Fluorescent & Hapten-Labeled Nucleotide Analogs

Direct Fluorescence Detection Labeling Reagents

Dideoxynucleotide AnalogsAvailable in package sizes indicated below. Larger quantities/bulk price quotations are available upon request.

Nucleotide Analogs & cDNA Microarray Analysis

PerkinElmer offers an extensive line of high quality fluorescent- and hapten-labeled nucleotides for applications using both direct and indirect detection.

Proprietary synthesis and purification process delivers:

• Consistently high purity (>95%)

• Consistent performance

• Superior results every time

Support all genomic applications:

• Microarrays

• Single nucleotide polymorphisms (SNPs)

• Nucleic acid blotting

• In situ hybridization

Choose the best combination of dye/hapten nucleotides foryour application. Custom synthesis and packaging is available.

Product Product No.

Fluorescein-12-ddATP NEL402001EA

Fluorescein-12-ddCTP NEL400001EA

Product Product No.

Fluorescein-12-ddGTP NEL403001EA

Fluorescein-12-ddUTP NEL401001EA

Fluorescein Labeled

(25 nmol package size) (1.0 mM concentration)

Product Product No.

ROX-ddATP NEL478001EA

ROX-ddCTP NEL477001EA

Product Product No.

ROX-ddGTP NEL479001EA

ROX-ddUTP NEL476001EA

ROX Labeled (Isomerically pure)

(2.5 nmol package size) (0.1 mM concentration)

Product Product No.

R6G-ddATP NEL490001EA

R6G-ddCTP NEL489001EA

Product Product No.

R6G-ddGTP NEL491001EA

R6G-ddUTP NEL488001EA

R6G Labeled (Isomerically pure)


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sNucleotide Analogs & cDNA Microarray AnalysisDirect Fluorescence Detection Labeling Reagents: Dideoxynucleotide Analogs



Product Product No.

TAMRA-ddATP NEL474001EA

TAMRA-ddCTP NEL473001EA

Product Product No.

TAMRA-ddGTP NEL475001EA

TAMRA-ddUTP NEL472001EA

TAMRA Labeled (Isomerically pure)


Product Product No.

Cyanine 3-ddATP NEL586001EA

Cyanine 3-ddCTP NEL584001EA

Cyanine 3-ddGTP NEL587001EA

Cyanine 3-ddUTP NEL585001EA

Product Product No.

Cyanine 5-ddATP NEL590001EA

Cyanine 5-ddCTP NEL588001EA

Cyanine 5-ddGTP NEL591001EA

Cyanine 5-ddUTP NEL589001EA

Cyanine Labeled


Fluorophore Labeled (Isomeric mixture)


Product Product No.

Texas Red®-5-ddATP NEL411001EA

Product Product No.

R110-ddATP NEL494001EA

R110-ddCTP NEL493001EA

Product Product No.

R110-ddGTP NEL495001EA

R110-ddUTP NEL492001EA

R110 Labeled


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Nucleotide Analogs & cDNA Microarray AnalysisDirect Fluorescence Detection Labeling Reagents: Deoxynucleotide Analogs



Deoxynucleotide AnalogsAll available in 25 nmol package sizes and 1.0 mM concentration unless noted. Larger quantities/bulk price quotations are available upon request.

Product Product No.

Cyanine 3-dUTP NEL578001EA

Cyanine 5-dUTP NEL579001EA

Diethylaminocoumarin-5-dUTP NEL455001EA

Fluorescein-12-dUTP NEL413001EA

Lissamine-5-dUTP NEL418001EA

Product Product No.

Tetramethylrhodamine-6-dUTP NEL416001EA

Texas Red®-5-dUTP NEL417001EA

Smart Pack dUTP NEL621001EA

(5 x Cyanine 3-dUTP and 5 x Cyanine 5-dUTP)

TTP Analogs

Product Product No.

Cyanine 3-dCTP NEL576001EA

Cyanine 5-dCTP NEL577001EA

Diethylaminocoumarin-5-dCTP NEL456001EA

Fluorescein-12-dCTP NEL424001EA

Product Product No.

Tetramethylrhodamine-6-dCTP NEL425001EA

Texas Red®-5-dCTP NEL426001EA

Smart Pack dCTP NEL620001EA

(5 x Cyanine 3-dCTP and 5 x Cyanine 5-dCTP)

dCTP Analogs

Product Product No.

Cyanine 3-dATP NEL592001EA

Cyanine 5-dATP NEL593001EA

Product Product No.

Fluorescein-12-dATP NEL465001EA

Texas Red®-5-dATP NEL471001EA

dATP Analogs

Product Product No.

Cyanine 3-dGTP NEL594001EA

Cyanine 5-dGTP NEL595001EA

Product Product No.

Fluorescein-12-dGTP NEL429001EA

dGTP Analogs

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sNucleotide Analogs & cDNA Microarray AnalysisDirect Fluorescence Detection Labeling Reagents: Ribonucleotide Analogs



Ribonucleotide AnalogsAll available in 25 nmol package sizes and 1.0 mM concentration unless noted.

Product Product No.

Cyanine 3-CTP* NEL580001EA

Cyanine 5-CTP* NEL581001EA

Fluorescein-12-CTP NEL434001EA

Product Product No.

Smart Pack CTP* NEL622001EA

(5 x Cyanine 3-CTP and 5 x Cyanine 5-CTP)

CTP Analogs

Product Product No.

Cyanine 3-ATP NEL596001EA

Cyanine 5-ATP NEL597001EA

Product Product No.

Fluorescein-12-ATP NEL439001EA

ATP Analogs

Product Product No.

Cyanine 3-GTP NEL598001EA

Cyanine 5-GTP NEL599001EA

Product Product No.

Fluorescein-12-GTP NEL496001EA

GTP Analogs

* Available as: 100 nmol package, 10.0 mM concentration.

Cyanine Smart Pack

Product Product No.

Cyanine 3-UTP* NEL582001EA

Cyanine 5-UTP* NEL583001EA

Fluorescein-12-UTP NEL414001EA

Product Product No.

Smart Pack UTP* NEL623001EA

(5 x Cyanine 3-UTP and 5 x Cyanine 5-UTP)

UTP Analogs

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Nucleotide Analogs & cDNA Microarray AnalysisIndirect Detection Labeling Reagents/MICROMAX Glass Slide Substrates



MICROMAX UltraClean™ & SuperChip™ Glass Slide SubstratesUltraClean™ I and SuperChip™ I premium glass slides are ready-to-use for cDNA microarray production. Manufactured using tightlycontrolled operation procedures, these slides give reliable, precise and accurate differential gene expression data.

Low intrinsic fluorescence.

Ultra-flat slides for accurate and precise signal generation.

Name Description Product No.

MICROMAX Glass Slides, UltraClean™ I 20 slides MPS692001KT

Shipped ambient. Store ambient.

MICROMAX Glass Slides, SuperChip™ I 20 slides MPS696001KT

Shipped ambient. Store ambient.

Indirect Detection Labeling ReagentsIndirect Chemiluminescence, Colorimetric, or Tyramide Signal Amplification (TSA) Detection Applications

Product Product No.

Aminodigoxigenin-9-dCTP NEL562001EA

Biotin-11-dATP NEL540001EA

Biotin-11-dCTP NEL538001EA

Product Product No.

Biotin-11-dGTP NEL541001EA

Biotin-11-dUTP NEL539001EA

DNP-11-dUTP NEL551001EA

Deoxynucleotide Analogs


Product Product No.

Aminodigoxigenin-9-ddGTP NEL573001EA

Biotin-11-ddATP NEL548001EA

Biotin-11-ddCTP NEL546001EA

Product Product No.

Biotin-11-ddGTP NEL549001EA

Biotin-11-ddUTP NEL547001EA

Dideoxynucleotide Analogs


Product Product No.

Biotin-11-ATP* NEL544001EA

Biotin-11-CTP NEL542001EA

Biotin-11-GTP* NEL545001EA

Product Product No.

Biotin-11-UTP NEL543001EA

DNP-11-UTP NEL555001EA

Ribonucleotide Analogs


* Available as: 25 nmol package, 1.0 mM concentration.

UltraClean™ I

Pristine glass surface for the production of coated slides

SuperChip™ I

Uniform, aminosilane substrate on UltraClean™ I glass slide

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Nucleotide Analogs & cDNA Microarray AnalysisMICROMAX Labeling Systems

MICROMAX Labeling Systems

Choosing a MICROMAX SystemChoose between MICROMAX direct labeling and TSA amplified systems by considering your level of experience with microarrays,the number of microarray assays you will run, and the types of genes you will study.

MICROMAX Direct Labeling Kits utilize conventional cDNA labeling protocols and direct fluorescence detection techniques. Recommended for users new to microarray technology.

MICROMAX ASAP RNA Labeling Kits contain all the key reagents to eliminate enzyme-mediated labeling bias.

MICROMAX TSA Labeling & Detection Kits, for experienced researchers successful with microarray technology,reduce starting RNA sample quantity requirements.

MICROMAX Direct Labeling Kit• Complete reagent system for direct cDNA labeling

and hybridization with Cyanine 3- or Cyanine 5- labeled dUTPs.

• Ideal for high volume applications using spotted microarrays.

MICROMAX ASAP miRNA Labeling Kit• A fast and easy system for the uniform labeling and

detection of in vivo synthesized miRNA.

• MICROMAX Accurate Sensitive and Precise (ASAP) miRNAChemical Labeling Kit enables microarray-based identifi-cation of miRNA targets, providing a high throughputalternative to Northern blotting of unparalleled sensitivity.

MICROMAX ASAP RNA Labeling Kit• The system ensures uniform labeling of two fluorophores

(Cyanine 3 and Cyanine 5) so that you get precise andaccurate results.

• Add a single reagent to your total RNA. Requires noenzyme addition, producing unbiased labeling for dual-color hybridizations.

TSA Microarray Labeling & Detection Kits• Contains key reagents necessary for labeling,

hybridization and detection for ultrasensitive geneexpression experiments on glass DNA microarrays.

• Uses PerkinElmer’s proprietary TSA amplification tech-nology to allow 50 to 200 times less starting material than with conventional cDNA labeling protocols.

• Works with RNA from either tissue sources or cell lines.

• Allows differential gene expression analysis of minutesamples such as tissue biopsies.

Name Description Product No.

MICROMAX Direct Labeling Kit - 10 reactions: 5 dual color labeling reactions MPS501001KT

Contains key reagents for direct cDNA labeling with cyanine nucleotides. 50 reactions: 25 dual color labeling reactions MPS502001KT

MICROMAX ASAP miRNA Labeling Kit 10 reactions: 5 dual color labeling reactions MPS545001KT

MICROMAX ASAP RNA Labeling Kit 50 reactions: 25 dual color labeling reactions MPS544001KT

MICROMAX TSA Labeling & Detection Kit 5 labeling experiments MPS521001KT

20 labeling experiments MPS522001KT

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Array Comparative Genomic HybridizationSpectralChip 2600/SpectralWare



Spectral Genomics SpectralChip 2600 SpectralChip 2600 arrays are 2600-element BAC arrays used to evaluate CGH or chromosomal abnormalities at locations approximately 1 megabase apart across the entirehuman genome. Our human BAC arrays generate a genome-wide molecular profile and quantification of chromosomalimbalances on a single chip.

Our arrays display high sensitivity with low background,facilitating image analysis for the qualitative analysis ofhybridization. DNA (obtained from blood, amniotic fluid,chronic villi or tissue samples) is analyzed directly byhybridization.

Key Features• Higher resolution molecular karyotyping: expands the res-

olution of genomic analysis by at least an order of magni-tude over conventional G-banding microscopic techniques.

• Use of array CGH for karyotyping provides high resolutionview of the entire genome: not limited by the requirementto pre-select the gene defects being analyzed, unlikeFluorescent In Situ Hybridization (FISH).

• No amplification of sample DNA is required prior tohybridization with the array spots.

• No harvesting of cells is required before analysis of chromosomal abnormalities, unlike conventionalmetaphase spread techniques for karyotyping.

• Arrays contain nearly 3,000 clones spanning the entirehuman genome: average one megabase intervals across the genome, mapped to a cytogenetic linear position.

• Fast turnaround: 16 hours to results.

Spectral Genomics SpectralWare Array AnalysisSystem SoftwareSpectralWare™ Array Analysis System software, used incombination with SpectralChip arrays, is the only commer-cially available, automated method to generate a genome-wide molecular karyotype profile in a single experiment.

SpectralWare software accepts data from a variety of arrayscanners, including PerkinElmer’s ScanArray product line. It converts two-color fluorescent dye signals into intensityratio profiles. The profiles for each chromosome are correlatedand displayed with ideograms of the chromosome to showthe loci of each feature detected in the genome. SpectralWaredisplays chromosomal deletions and amplifications in aunique graphic presentation for rapid interpretation of results.

Features & Benefits • Web-based: simple deployment and administration.

• Analysis logic downloads to your PC and runs on your data: faster analysis; your data remain local, secure and confidential.

Generate genome wide molecular profiles in a single experiment with our SpectralGenomics aCGH arrays and associated software. Our Array CGH (Comparative GenomeHybridization) technology circumvents the considerable limitations of using metaphasespreads by using microarray analysis.

Traditional karyotyping methods for detecting genomic variation, such as FISH or G-bandingare well established for detecting chromosomal aberrations, but they don’t provide the speed,throughput, or resolution required for today’s cytogenetic laboratories.

Our Spectral Genomics technology* takes karyotyping to a new level of performance and ease-of-use. You can survey the entirehuman genome with high resolution and specificity, and obtain abundant, reproducible data on the genomic loci of chromosomalamplifications, deletions and rearrangements.

* Protected by U.S. Patent No. 6,048,695 and foreign equivalents.


SpectralChip 2600 Includes 2 Bacterial Artificial SC2600

Human BAC Array Kit Chromosome (BAC) arrays slides

Keep all reagents except containing ~2600 BAC clones

HYB Buffer II at -20 °C from the RPCI BAC library,

until needed. Keep all each spotted in duplicate.

arrays in a dessicator Also includes hybridization

at room temperature and labeling buffers and

until needed. Dnase/Rnase-free solution.

Array Comparative Genomic Hybridization

ScanArray Gx

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sSNP Detection SystemsaCGH (cont.)/AcycloPrime-FP SNP Detection Systems



SNP Detection Systems

AcycloPrime-FP SNP Detection SystemsOur system is a simple and robust method for SNP detectionthat uses techniques and protocols familiar to every laboratory.

• Based on the process invented, published and patented byDrs. Chen, Levine and Kwok, the systems use fluorescencepolarization to analyze single base extension reactionproducts for SNP analysis.1,2,3

• Homogeneous, microplate-based assay that eliminateswashing and separation steps.

• All kits include a specially formulated reagent optimizedfor use with AcycloPrime™-FP for the PCR clean-up step.

• Optimized for use on our VICTOR3 V Multilabel PlateReader, which can be used to analyze SNPs in a highthroughput format. You can also use any commerciallyavailable fluorescence polarization reader that has the required sensitivity.

Features & Benefits• Easy to use: all reagents are provided in the kit along

with specific instructions.

• Simple protocol: homogeneous assay with no plate-to-plate transfers, centrifugation, wash or separation steps.

• Better sensitivity and accuracy than other methods:novel AcycloTerminators and AcycloPol™ enzyme provide results that are superior to dye-ddNTP’s andThermo Sequenase.

• Fast time to results: high throughput read times (15 minutes with the VICTOR3 V).

• More affordable than most other technologies:perfect for labs just starting SNP Detection.

PerkinElmer provides a total solution for SNPs scoring, suitable for labs of all sizes.

The AcycloPrime-FP method is a simple, homogeneous and robust approachto SNP detection using microplates and fluorescence polarization detection.

1 Chen, X., Levine, L. and Kwok, P-Y., “Fluorescence polarization in homogeneous nucleic acid analysis”, Genome Research (1999) 9, 492–298.

2 Hsu, T.M., Chen, X., Duan, S., Miller, R.D. and Kwok, P-Y., “Universal SNP genotyping assay fluorescence polarization detection”, BioTechniques (2001) 31, 560–570.

3 Gardner, A.F. and Jack, W.E. “Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases”, Nucleic Acids Research (2002) 30, 605–613.

• Server provides comprehensive human genome annotationfor analysis results: no need to download the entirehuman genome annotation set.

• Color-enhanced data visualization via chromosomalideograms, ratio plots, hi-lighted gains/deletions, detailed tabular view of clones overlapping the defects.

• Provides automated assistance in detecting and characterizing chromosomal defects.

• Semi-automated report generation: built-in Report editorauto-populated with starting information; speeds upreporting but still allows customized annotation.

For Research Use Only. Not for use in diagnostic procedures.

Upon completion of processing, slides are scanned and imaged with the ScanArray Gx. Files are then imported into SpectralWare where data

is presented visually and mapped to individual chromosomes. Changes in reference vs. test are indicated by a deflection from the central axis.

ScanArray Gx

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AcycloPrime-FP SNP Detection Systems/SNP Data Analysis Software



AcycloPrime-FP SNP Detection Systems can detect all possible combinations for SNPs. Each kit contains a terminator mix with theappropriate dye-labeled AcycloTerminators that will be incorporated into the SNP primer. These correspond to the alleles present atthe SNP to be detected.

Name Package Size Terminator Mix (R110/TAMRA) Product No.

AcycloPrime II-FP SNP Detection Kit, G/A 1K reactions 1K G/A ACP101A001KT

10K reactions 10K G/A ACP101B001KT

100K reactions 100K G/A ACP101C001KT

AcycloPrime II-FP SNP Detection Kit, G/C 1K reactions 1K G/C ACP102A001KT

10K reactions 10K G/C ACP102B001KT

100K reactions 100K G/C ACP102C001KT

AcycloPrime II-FP SNP Detection Kit, G/T 1K reactions 1K G/T ACP103A001KT

10K reactions 10K G/T ACP103B001KT

100K reactions 100K G/T ACP103C001KT

AcycloPrime II-FP SNP Detection Kit, C/A 1K reactions 1K C/A ACP104A001KT

10K reactions 10K C/A ACP104B001KT

100K reactions 100K C/A ACP104C001KT

AcycloPrime II-FP SNP Detection Kit, C/T 1K reactions 1K C/T ACP106A001KT

10K reactions 10K C/T ACP106B001KT

100K reactions 100K C/T ACP106C001KT

AcycloPrime II-FP SNP Detection Kit, A/T 1K reactions 1K A/T ACP109A001KT

10K reactions 10K A/T ACP109B001KT

100K reactions 100K A/T ACP109C001KT

AcycloPrime II-FP SNP Detection Combination Kit 10K reactions 3K G/A, 3K C/T, 1K G/C, ACP113B001KT1K G/T, 1K C/A, 1K A/T

100K reactions 30K G/A, 30K C/T, 10K G/C, ACP113C001KT10K G/T, 10K C/A, 10K A/T

SNP Data Analysis Softwarewww.SNPscoring.comPlease visit www.SNPscoring.com, your one stop AcycloPrime-FP SNP detection resource for:

TDI method overview

Primer design resource

AcycloPrime-FP literature references

Detailed instrumentation and software information

Molecular Biology Products - POLATOM · patented Tyramide Signal Amplification (TSA ™)...

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Transcript of Molecular Biology Products - POLATOM · patented Tyramide Signal Amplification (TSA ™)...