Module 2 - Wayne State UniversityBiohazard An agent of biological origin that has the capacity to...
Transcript of Module 2 - Wayne State UniversityBiohazard An agent of biological origin that has the capacity to...
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Module 2
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BiohazardAn agent of biological origin that has the capacity to produce deleterious effects on humans, i.e. microorganisms, toxins and allergens derived from those organisms; and allergens and toxins derived from higher plants and animals.
Principles Definition
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IntroductionDevelopment of Biosafety Practices
� 1941 - Meyer and Eddie� 74 lab associated brucellosis infections
in US� 1949 - Sulkin and Pike
� 222 viral infections (21 fatal)� Only 27% related to known accidents
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IntroductionDevelopment of Biosafety Practices
� 1951, 1965, 1976 - Sulkin and Pike� Surveys for lab-associated infections� More than 5,000 labs� Cumulative total of 3,921 cases cited� Most commonly reported:
• Hepatitis• Tuberculosis• Typhoid
• Venezuelan Equine Encephalitis• Brucellosis • Tularemia
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� 1951,1965, 1976 - Sulkin and Pike� Fewer than 20% associated with known
accidents� Exposure to infectious aerosols plausible
(but unconfirmed) for >80% of reported cases
IntroductionDevelopment of Biosafety Practices
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Protection:� workers� “products”� co-workers� lab support personnel� environment
IntroductionWhy Biosafety Practices?
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IntroductionChain of Infection
Reservoir of pathogenReservoir of pathogen
Portal of escapePortal of escape
TransmissionTransmission
Route of entry/infectious doseRoute of entry/infectious dose
Susceptible hostSusceptible host
Incubation periodIncubation period
Risk Assessment
PPE
Immunization
Surveillance
Practices/
Equipment
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Principles General Lab Requirements
� Knowledgeable supervisor
� Knowledgeable personnel� Aware of potential hazards� Proficient in practices &
techniques� Lab specific biosafety
manual
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Principles General Lab Requirements
� Biosafety Levels (BSLs)� Laboratory Practice and Technique
� Standard Practices� Special Practices
� Safety Equipment (Primary Barriers)� Facility Design and Construction
(Secondary Barriers)
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Principles General Lab Requirements
� Biosafety cabinets (BSCs) - BSL 2/3� Personal protective clothing
� Gloves� Gowns� Eye and face protection
� Pipetting Devices � Safety centrifuge cups and rotors
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BiosafetyThe application of combinations of laboratory practice and procedure, laboratory facilities, and safety equipment when working with potentially infectious microorganisms.
Principles Definition
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� BSL1 - agents not known to cause disease.� BSL2 - agents associated with human
disease.� BSL3 - indigenous/exotic agents associated
with human disease and with potential for aerosol transmission.
� BSL4 - dangerous/exotic agents of life threatening nature.
Principles Biosafety Levels
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Biosafety Level 1Introduction
uitable for work involving well-characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment.
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Biosafety Level 1 Introduction
Examples:� Bacillus subtilis� Naegleria gruberi� Infectious canine hepatitis virus� E. coli
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Biosafety Level 1 Facility Design (Secondary Barrier)
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Biosafety Level 1 Facility Design (Secondary Barrier)
Requirements:� Laboratories have doors� Sink for hand washing� Work surfaces easily cleaned� Bench tops are impervious to water� Sturdy furniture� Windows fitted with flyscreens
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Biosafety Level 1 Facility Design (Secondary Barrier)
Easily cleanedand
decontaminated
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Requirements:� Location - not separated � Structure - normal construction� Ventilation - none
Biosafety Level 1 Facility Construction (Secondary Barrier)
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Biosafety Level 1 Standard Microbiological Practices
� Restrict or limit access when working
� Prohibit eating, drinking and smoking
� Prohibit mouth pipetting
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Biosafety Level 1 Standard Microbiological Practices
Use mechanical
pipettingdevices
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Biosafety Level 1 Standard Microbiological Practices
Wash hands
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Biosafety Level 1 Standard Microbiological Practices
� Minimize splashes and aerosols� Decontaminate work surfaces daily� Decontaminate wastes � Maintain insect & rodent control
program
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Biosafety Level 1Safety Equipment (Primary Barriers)
Protective clothing� Lab coat � Gloves
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Biosafety Level 1Safety Equipment (Primary Barriers)
Personal protective equipment� Face protection� Eye protection
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Biosafety Level 1 Special Practices
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� Supervisor� Scientist with general training in
microbiology or related science
� Lab Personnel� Specific training in lab procedures
Biosafety Level 1 Training Requirements
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Biosafety Level 2Introduction
uitable for work involving agents of moderate potential hazard to personnel and the environment.
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Biosafety Level 2Introduction
Examples:� Measles virus� Salmonellae� Toxoplasma spp.� Hepatitis B virus
* Immunization or antibiotic treatment is available
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Biosafety Level 2Introduction
Examples:� Bloodborne pathogens� Human body fluids/particularly when visibly
contaminated with blood
* Extreme precaution with contaminated needles or sharp instruments
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Biosafety Level 2 Facility Design (Secondary Barriers)
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Biosafety Level 2 Facility Design (Secondary Barriers)
Requirements:� Laboratories have lockable doors� Sink for hand washing� Work surfaces easily cleaned� Bench tops are impervious to water� Sturdy furniture
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Biosafety Level 2 Facility Design (Secondary Barriers)
Requirements (cont.):� Biological safety cabinets installed as
needed� Adequate illumination� Eyewash readily available� Air flows into lab without re-circulation to
non-lab areas� Windows fitted with flyscreens
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Biosafety Level 2 Facility Design (Secondary Barrier)
Restrictedaccess when
work inprogress
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Biosafety Level 2Laboratory Facilities (Secondary Barriers)
� BSL-1 Facilities PLUS:� Autoclave
available� Eyewash station
available
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Requirements:� Location - separated from public areas � Structure - normal construction� Ventilation - directional
Biosafety Level 2 Facility Construction (Secondary Barrier)
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Biosafety Level 2Standard Microbiological Practices
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Biosafety Level 2Safety Equipment (Primary Barriers)
� Use biosafety cabinets (class II) for work with infectious agents involving:� Aerosols and splashes� Large volumes � High concentrations
2.4
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Biosafety Level 2Safety Equipment (Primary Barriers)
� Class II BiosafetyCabinet
� Airflow
2.4
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Biosafety Level 2Safety Equipment (Primary Barriers)
� Class II Biosafety Cabinet � Equipment layout
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Biosafety Level 2Safety Equipment (Primary Barriers)
� Class II BiosafetyCabinet
� Technique
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Biosafety Level 2 Special Practices
Supervision� Supervisor is a competent scientist with
increased responsibilities� Limits access if immunocompromised� Restricts access to immunized
Lab Personnel� Aware of potential hazards� Proficient in practices/techniques
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Biosafety Level 2Special Practices
Needles & Sharps Precautions� Use sharps containers� DON’T break, bend, re-sheath or reuse
syringes or needles
2.4
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Biosafety Level 2Special Practices
Needles & Sharps Precautions (cont.)� DON’T place needles or sharps in office
waste containers
2.4
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Biosafety Level 2Special Practices
Needles and Sharps Precautions (cont.)� DON’T touch broken glass with hands
2.4
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Biosafety Level 2Special Practices
Needles and Sharps Precautions (cont.)� Use plasticware
2.4
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Biosafety Level 2Special Practices
� Policies and procedures for entry� Biohazard warning signs� Biosafety manual specific to lab� Training with annual updates
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Biosafety Level 2Special Practices
� Use leak-proof transport containers
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Biosafety Level 2Special Practices
� Immunizations� Baseline serum samples
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� Decontaminate work surfaces� Report spills and accidents� No animals in laboratories
Biosafety Level 2Special Practices
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Suitable for work with infectious agents which may cause serious or potentially lethal disease as a result of exposure by the inhalation route.
Biosafety Level 3Introduction
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� Exposure potential to pathogens spread by aerosol
� Infection serious, possibly lethal� Examples:
� M. tuberculosis� St. Louis encephalitis virus� Coxiella burnetii
Biosafety Level 3Introduction
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Biosafety Level 3 Laboratory Facilities (Secondary Barriers)
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Biosafety Level 3Laboratory Facilities (Secondary Barriers)
� BSL-1 and 2 Facilities PLUS:� Separate building or isolated zone
� Double door entry
� Directional inward airflow
� Single-pass air; 10-12 air changes/hour
2.5
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Biosafety Level 3Laboratory Facilities (Secondary Barriers)
� BSL-1 and 2 Facilities PLUS (cont.):
� Enclosures for aerosol generating equipment
� Room penetrations sealed� Walls, floors and ceilings are water
resistant for easy cleaning
2.5
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Biosafety Level 3Laboratory Facilities ( Secondary Barriers)
� BSL-1 and 2 Facilities PLUS:� Vacuum lines protected with liquid
disinfectant traps or HEPA filters
2.5
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Facility Design(Tertiary Barriers)
CDCContainmentLaboratory
2.5
BSL 4
BSL 3
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Facility Design(Tertiary Barriers)
� Lab structure� Lab ventilation
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Biosafety Level 3Standard Microbiological Practices
2.5
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Biosafety Level 3Safety Equipment (Primary Barriers)
� BSL-1 and 2 Safety Equipment PLUS:� BSC class
II or III to manipulate infectious material
2.5
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Biosafety Level 3Safety Equipment (Primary Barriers)
� BSL-1 and 2 Safety Equipment PLUS:� Respiratory protection
may be indicated
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� BSL-2 Special Practices PLUS:� Work in certified BSC� Use bioaerosol-
containing equipment� Decontaminate spills
promptly
Biosafety Level 3Special Practices
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Biosafety Level 3 Special Practices
Supervision� Supervisor is a competent scientist
experienced working with agents�Establishes criteria for entry�Restricts access�Develops policies/procedures� Trains lab personnel
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Biosafety Level 3 Special Practices
Lab Personnel� Strictly follow guidelines� Demonstrate proficiency� Receive appropriate training� Report incidents� Participate in medical surveillance
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Suitable for work with dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease.
Biosafety Level 4Introduction
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� Exposure potential to pathogens spread by aerosol or with unknown risk of transmission
� Infection possibly lethal� Examples:
� Ebola Zaire� Sin Nombre virus� Rift Valley Fever
Biosafety Level 4Introduction
Ebola ZaireEbola Zaire2.6
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Biosafety Level 4Laboratory Facilities (Secondary Barriers)
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Biosafety Level 4Laboratory Facilities (Secondary Barriers)
� BSL-1, 2, and 3 Facilities PLUS:� Separate building or
isolated zone� Double door entry� Directional inward airflow� Single-pass air� Dedicated supply and
exhaust, vacuum, and decon systems
2.6
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Biosafety Level 4Laboratory Facilities (Secondary Barriers)
� BSL-1, 2 and 3 Facilities PLUS (cont.):
� Enclosures for aerosol generating equipment
� Double door autoclaves� Room penetrations sealed� Walls, floors and ceilings are sealed to
form an internal seal
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Biosafety Level 4Laboratory Facilities (Secondary Barriers)
� BSL-1, 2 and 3 Facilities PLUS (cont.):� Connecting inner and outer doors -
interlocked to prevent simultaneous opening
� Liquid effluents are decontaminated by an approved method and certified before discharge
� Communication system between inside and outside of the lab
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Biosafety Level 4Laboratory Facilities ( Secondary Barriers)
� BSL 1, 2, and 3 Facilities PLUS:� Emergency breathing air� Emergency generator� Emergency exit
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Biosafety Level 4Facility Design
CDCContainmentLaboratory
BSL 4BSL 4
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Biosafety Level 4Facility Design
� Clean� “Hot”
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Biosafety Level 4Standard Microbiological Practices
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Biosafety Level 4Safety Equipment (Primary Barriers)
� BSL 1, 2, and 3 Safety Equipment PLUS:� Class II (B2) or III biological safety cabinets
to manipulate infectious material
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Biosafety Level 4Safety Equipment (Primary Barriers)
� BSL 1, 2, and 3 Safety Equipment PLUS:
� Positive pressure personnel suit
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� BSL 3 Special Practices PLUS:
� Decontaminate all liquid effluent
� Decontaminate all solid wastes
Biosafety Level 4Special Practices
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Biosafety Level 4Special Practices
� Controlled access � Personnel enter facility through
changing room where they are required to change into laboratory clothing
� Showers are required upon exit from the laboratory
� Supplies enter lab through double-door autoclave or fumigation chamber
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Biosafety Level 4 Special Practices
Supervision� Supervisor is a competent scientist
trained and experienced working with agents�Establishes criteria for entry�Restricts access�Develops policies/procedures� Trains lab personnel
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Biosafety Level 4 Special Practices
Lab Personnel� Strictly follow guidelines� Demonstrate proficiency� Receive appropriate training� Report incidents� Receive available immunizations� Participate in medical surveillance
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Principles of BiosafetySummary
� BSL 1 - 4� Standard Practices� Special Practices� Safety Equipment (Primary Barriers)� Laboratory Facilities (Secondary
Barriers)� Building (Tertiary Barriers)
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Biological Safety CabinetsPurpose
� Product protection� Personal protection� Environmental
protection
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Biological Safety CabinetsTypes
A. Class I� inward airflow protects worker� exhaust to outside (w/wo HEPA filter)
B. Class II� worker, product, environmental protection� “sterile” work area� use for work with aerosol-transmissible micro-
organisms� use also for tissue culture/ virology
C. Class III� totally enclosed, ventilated, air-tight� suitable for work with BSL3/4 agents 2.7
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Biological Safety CabinetsTypes
Class II� Type A 30% exhausted to room� Type B3 30% exhausted to outside� Type B1 70% exhausted to outside� Type B2 100% exhausted to outside
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Biological Safety CabinetsComponent
HEPA Filter� “High efficiency particulate air” filter� Traps particulates only; chemicals,
fumes, vapors pass through� Traps particulates 0.3u
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Biological Safety CabinetsComponent
HEPA Filter� Metal or wood framed� Continuous sheet of
flat filter medium with aluminum separators
� Gasket sealed� Adhesive bond
between filter pack and frame
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� Isolated from other work areas
� Removed from high traffic areas
� Away from airflow ducts
� Away from laboratory entry doors
Biological Safety CabinetsOperating Location
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Exhaust
Intake100
ft/min
Typical Class II
Biological Safety CabinetsAirflow
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1. Load BSC with all needed supplies.2. Turn BSC on and allow to run for 10-15 minutes.3. Check inward airflow with a piece of tissue.4. Enter straight into cabinet and perform work in a
slow, methodical manner.5. At end of work, decontaminate all items to be
taken out of cabinet.6. Decontaminate interior of BSC.7. Allow cabinet to run for 10-15 minutes.8. Shut off.
Biological Safety CabinetsOperating Procedure
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■ Always enter straight into cabinet - no sweeping motions
■ Place materials well within the cabinet - not on front grill
■ Place discard pan within cabinet■ Watch for disruptions of laminar air flow■ Decontaminate materials before removal
from cabinet
Biological Safety CabinetsSafe Operation
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■■ Not designed for chemical use■ May use for non-volatile toxic chemicals or
low-level radioactive materials■ May use for “minute” amounts of volatile
chemicals■ Ensure annual certification■ Place all work materials into cabinet before
starting
Biological Safety CabinetsSafe Operation
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CAUTIONS� Chemicals may damage HEPA filter
� Exposure risk - chemical/infectious agents� Volatile chemicals NOT retained by HEPA
filter� Exposes personnel if not exhausted
� BSC fans NOT spark proof� Chemical use may result in fire/ explosion� Never use NFPA 4 flammables
Biological Safety CabinetsSafe Operation
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Centrifuges
TypesTypes
MicrocentrifugesMicrocentrifuges
Low/high speedLow/high speed
UltracentrifugesUltracentrifuges
Speeds (rpm)Speeds (rpm)
~15,000~15,000
2,000 2,000 –– 20,00020,000
~ 120,000~ 120,000
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CentrifugesHazards
�� Mechanical failure of machineMechanical failure of machine�� Lab equipment failure (tubes etc.)Lab equipment failure (tubes etc.)�� Aerosol generationAerosol generation�� Operator errorOperator error
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1. Check tubes for cracks/chips.2. Use matched sets of tubes, buckets etc.3. Tightly seal all tubes and safety cups.4. Ensure that rotor is locked to spindle
and bucket seated.5. Close lid during operation.6. Allow to come to complete stop before
opening.
CentrifugesOperating Procedure
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� Use safety cups whenever possible� Disinfect weekly and after all spills or
breakage's� Lubricate O-rings and rotor threads
weekly� Do not use rotors that have been dropped� Contact your centrifuge rep for specific
information
CentrifugesSafe Operation
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� Recommendations of the United Nations Committee on Dangerous Goods
Shipping Biological SpecimensGuideline Documents
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� PHS: 42 CFR Part 72. � DOT: 49 CFR Part 171-178� USPS: Domestic Mail
Manual� IATA: International Air
Transport Association� ICAO: International Civil
Aviation Organization
Shipping Biological SpecimensRegulations
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Definition� Contains or has high probability of
containing an infectious material…known or reasonably believed to cause disease in humans or animals� virus, prion, genetic elements� bacterium, rickettsia, parasite, fungus
� Contains a microbial toxin known to be pathogenic
Shipping Biological SpecimensInfectious Substance
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Packaging� Primary Container
� Positive seal
� Absorbent material
Shipping Biological SpecimensInfectious Substance
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Packaging� Secondary packaging
� Watertight/leakproof
Shipping Biological SpecimensInfectious Substance
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Packaging� Between Secondary and Outer
Container � List of Contents� Shippers label
� Name� Address� Phone number
Shipping Biological SpecimensInfectious Substance
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Packaging� Outer container
Shipping Biological SpecimensInfectious Substance
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Packaging� Performance tests
� 49 CFR 178.609
Shipping Biological SpecimensInfectious Substance
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Packaging label
Shipping Biological SpecimensInfectious Substance
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Shipping Biological SpecimensInfectious Substance
Containers
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DefinitionHuman or animal material…collected
for the purpose of diagnosis or research….not known to contain viable infectious agents
Shipping Biological SpecimensClinical Specimen
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Packaging� Primary receptacle
� Positive seal
� Biohazard label
� Absorbent material
Shipping Biological SpecimensClinical Specimen
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Packaging� Between the secondary and outer
packaging� List of contents
Shipping Biological SpecimensClinical Specimen
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Packaging� Outer packaging
Shipping Biological SpecimensClinical Specimen
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Package Label
Shipping Biological SpecimensClinical Specimen
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Packaging� Performance test
Shipping Biological SpecimensClinical Specimen
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� Biosafety Level Descriptions� Standard Practices & Principles� Special Practices & Procedures� Containment Devices� Facility Design
� Animal Safety Practices� Agent Summary Statements
Biosafety ManualsComponents
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� Equipment Descriptions� Specimen Handling� Security� Waste� Special Lab Practices
� Tissue culture� Toxins
Biosafety ManualsComponents
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DecontaminationDefinition
� SterilizationThe use of a physical or chemical procedure
to destroy all microbial life, including large numbers of highly resistant bacterial spores.
2.10
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� DisinfectionThe use of a physical or chemical procedure
to virtually eliminate all recognized pathogenic microorganisms but not all microbial forms (bacterial endospores) on inanimate objects.
2.10
DecontaminationDefinition
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� AntisepsisA germicide that is used on skin or living
tissue for the purpose of inhibiting or destroying microorganisms.
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DecontaminationDefinition
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� Degree of microbial killing required� Nature of item/surface to be treated� Ease of use� Safety � Cost
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DecontaminationAgent Selection
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� Type of organism� Number of organisms� Amount of organic material present� Type & configuration of material to be treated� Type & concentration of germicide� Time and temperature or exposure� pH� Humidity
2.10
DecontaminationAgent Efficacy
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� Heat� Chemical � Radiation
2.10
DecontaminationMethods
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� Types� Moist – steam� Dry� Incineration
*The most effective method of sterilization
2.10
DecontaminationHeat
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� Steam sterilization practices� Ensure proper functioning of autoclave� Vessels should not be capped or plugged� Large loads require longer contact time� Excessive amounts of liquid should not be
added to load
2.10
DecontaminationHeat
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� Steam sterilization verification� Direct assay� Thermocouples� Chemical indicators� Biological indicators
(Bacillus stearothermophilis)
2.10
DecontaminationHeat
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� Dry heat sterilization� Denaturation of proteins: 1600 – 1700 C/2-4
hours� Effective on impervious non-organic
materials like glass
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DecontaminationHeat
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� Incineration� Method of choice
for animal carcasses
� Requires certified incinerator
2.10
DecontaminationHeat
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� Types� Liquids, I.e. chlorox,
hydrogen peroxide� Gases, I.e. ethylene
oxide
2.10
DecontaminationChemical
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� Agent selection - complexity� Over 14,000 registered products� Over 300 active ingredients� 14 ingredients present in 92% of products
2.10
DecontaminationChemical
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� Agent selection - activity� HLD – high level disinfection� ILD – intermediate level disinfection� LLD – low level disinfection
2.10
DecontaminationChemical
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� High level disinfection - sporocides� Kills all microorganisms except high
numbers of bacterial spores� Require 5-10 min. exposure� Examples: aldehydes, hydrogen peroxide,
paracetic acid
2.10
DecontaminationChemical
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� Intermediate level disinfection -tuberculocides� Kills M. tuberculosis var. bovis and all
vegetative bacteria, fungi, and most viruses
� Require minimum 20 min. exposure� Examples: phenolics, iodophores, chlorine
compounds, alcohols
2.10
DecontaminationChemical
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� Low level disinfection – hospital germicides used for housekeeping� Kills most vegetative bacteria and some
fungi, but not M. tuberculosis var. bovis� Require minimum 20 min. exposure� Examples: quartenary ammonium
compounds
2.10
DecontaminationChemical
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Bacterial SporesB. subtilis
MycobacteriumMTB var. bovis
Non-lipid VirusesPolio-Rhino-
FungiCryptococcus sp,Candida sp.
Vegatative BacteriaPseudomonas sp.Staphylococcus sp.Salmonella sp.
Lipid VirusesHerpesCMVHBVHIV 2.10
DecontaminationSummary
SterilizationHLD
LLD
ILD
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� General Lab Use - Hypochlorite Solutions� Large Spills/Large Organic Load
� undiluted from bottle� Small Spills/Virus Inactivation
� 10% - 1:9� General Surface Disinfection
� 1% - 1:99
2.10
DecontaminationChemical
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Decontamination
2.10
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2.11
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Biological Waste
� Types� cultures, stocks, isolates� materials containing or contaminated with
blood� sharps� pipettes, wrappers, tips� All materials used in the lab
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Biological Waste
� puncture-proof, leak-proof, sealablereceptacles
� avoid over-filling� dispose properly
2.11
�Disposal
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�� DisposalDisposal- Never place lab waste into office waste containers- Place sharps into “sharps” container- Line discard containers with autoclave bag- Decontaminate discard pans before they leave thelab:
Biological Waste
2.11
1. Disinfect outside2. Label3. Tape ends with
autoclave4. Tape5. Secure for transport to
autoclave
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Biological Waste
Decontamination� To render the object/material safe by
reducing or removing the bioburden � Methods
� chemical ... match, contact time� physical ... Heat, steam and pressure� incineration� other choices, i.e. shredding + chemical
2.11
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2.12
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Medical SurveillanceCriteria
� Based on risk assessment� Pre-placement
� evaluate physical requirements� Periodic review
2.12
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� The probability of infection� Implies an estimate of numbers exists� Predict an outcome given similar events
Medical SurveillanceRisk Assessment
2.12
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� What is the natural host?� Does agent cross species barriers?� Wild-type agent or attenuated?� Infectious for normal healthy adult?� What if adult is immunocompromised?
Medical SurveillanceRisk Assessment
2.12
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� Mode of transmission?� contact� fomites� mucous membrane exposure� ingestion� inoculation or insect bites� inhalation� sex
Medical SurveillanceRisk Assessment
2.12
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� Volume being manipulated?� Concentration of agent?� Infectious dose?� Past history of lab-associated infection?� Secondary spread in community?
Medical SurveillanceRisk Assessment
2.12
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� Prophylaxis� Immunizations available?� Pharmaceuticals?� Effectiveness?
� Post-Exposure� Anti-microbial agents?� Pharmaceuticals?� Effectiveness?
Medical SurveillanceRisk Assessment
2.12
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� Dealing with an unknown agent?� epidemiological data� patterns parallel to other agents� data from animal studies� route of infection
Medical SurveillanceRisk Assessment
2.12
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� Top management� overall safety policy� resource allocation
� Supervisor� implement policies� training, practices & procedures, access
� Workers� strict & rigorous attention to details of
practices and procedures� report incidents and exposures
Medical SurveillanceRisk Management
2.12
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� Occupational Health Clinic� Immunizations, chemotherapy� Medical surveillance programs� Incident (emergency) response� Incident investigation
Medical SurveillanceRisk Management
2.12
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2.13
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1. Alert co-workers2. Clean exposed surface with soap/water,
eyewash (eyes), or saline (mouth)3. Apply first aid and treat as an emergency4. Notify supervisor or security desk (after
hours)5. Report to medical clinic for
treatment/counseling
Emergency ResponsePersonal Contamination
2.13
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Emergency ResponseSurface Contamination
1. Alert co-workers2. Define/isolate contaminated area 3. Put on appropriate PPE4. Remove glass/lumps with forceps or scoop5. Apply absorbent towel(s) to spill; remove bulk
& reapply if needed6. Apply disinfectant to towel surface6. Allow adequate contact time (20”)8. Remove towel, mop up; clean with alcohol or
soap/water9. Properly dispose of materials10. Notify supervisor
2.13