Module 2 - Infusion Safety

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    Protocol For Inserting A Peripheral Line

    Only staff nurses/trained medical professionalswith assistance will insert a peripheral cannula.

    All peripheral lines should be replaced in 72

    hrs. (96 hrs if vialon material used)

    Sterile dressing should be reinforced as and

    when necessary.

    Make a record of person inserting peripheral

    line, including time and date of insertion.

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    Cannulating staff nurses and head nurses should

    know infusion therapy process model. Anatomy & Physiology of the part to be

    cannulated should be known

    Patient consideration (thin, fat, hydration status)

    Therapy consideration Initiation of therapy

    Dressing

    After care of catheter

    Managing complications

    Protocol For Inserting A Peripheral Line

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    1. Successful completion

    of infusion therapy

    2. Reduced catheter

    related complications

    3. Minimized number of

    venipunctures per

    patient

    4. Reduced supplyrelated costs

    5. Reduced labor related

    costs

    6. Patient satisfaction

    7. Reduced needlestick

    injuries

    Outcomes

    Patient

    Considerations

    Device

    Selection

    Site

    Preparation

    Site

    Maintenance

    Site Assessment

    & EvaluationTherapy

    Considerations

    Clinician

    ConsiderationsInsertion

    Considerations

    Purpose

    Administration Rate

    Duration

    Nature of Fluids/Meds Insertion Difficulty

    Access Limitations

    Vein

    Skin Status

    Interventional Radiologist

    Insertion Angle

    Insertion Speed

    Skin Traction

    Vein Dilation

    Safety Practices

    Needle Disposal

    Catheter Advancement

    Infection Control Practices

    Education Needs

    Disease State

    Orientation

    Care Setting

    MobilityAge

    Therapy Considerations

    Risk Assessment

    Device Material

    Safety Features

    Cost-in-Use

    Patient Preferences

    Antimicrobial Awareness

    Appropriate Application

    Local AnestheticHair Removal Asepsis

    Documentation

    Flushing Protocols

    Monitor/Assessment

    Dressing Integrity

    Administration Set Protocols

    Maintenance Protocols

    Catheter Stabilization

    Delivery Equipment Requirements

    Types

    Allergies

    Gauge & Length

    Complication Prevention

    BD DECISIV

    Process Model-

    for Safe InfusionSpecialized Areas

    Generalist

    Physician

    IV Specialist

    Copyright 1997

    Updated: March

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    1. Successful completion

    of infusion therapy

    2. Reduced catheter

    related complications

    3. Minimized number of

    venipunctures per

    patient

    4. Reduced supplyrelated costs

    5. Reduced labor related

    costs

    6. Patient satisfaction

    7. Reduced needlestick

    injuries

    Outcomes

    Patient

    Considerations

    Device

    Selection

    Site

    Preparation

    Site

    Maintenance

    Site Assessment

    & EvaluationTherapy

    Considerations

    Clinician

    ConsiderationsInsertion

    Considerations

    Purpose

    Administration Rate

    Duration

    Nature of Fluids/Meds Insertion Difficulty

    Access Limitations

    Vein

    Skin Status

    Interventional Radiologist

    Insertion Angle

    Insertion Speed

    Skin Traction

    Vein Dilation

    Safety Practices

    Needle Disposal

    Catheter Advancement

    Infection Control Practices

    Education Needs

    Disease State

    Orientation

    Care Setting

    MobilityAge

    Therapy Considerations

    Risk Assessment

    Device Material

    Safety Features

    Cost-in-Use

    Patient Preferences

    Antimicrobial Awareness

    Appropriate Application

    Local AnestheticHair Removal Asepsis

    Documentation

    Flushing Protocols

    Monitor/Assessment

    Dressing Integrity

    Administration Set Protocols

    Maintenance Protocols

    Catheter Stabilization

    Delivery Equipment Requirements

    Types

    Allergies

    Gauge & Length

    Complication Prevention

    BD DECISIV

    Process Model-

    for Safe InfusionSpecialized Areas

    Generalist

    Physician

    IV Specialist

    Copyright 1997

    Updated: March

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    Choose correct catheter

    COLOUR COMMON APPLICATIONS SIZE/GAUGE CRYSTALLOID PLASMA BLOOD SANG

    OrangeUsed in theatres or emergency for rapid

    transfusion of blood or viscous fluids14G 16.2 13.5 10.3

    GreyUsed in theatres or emergency for rapid

    transfusion of blood or viscous fluids16G 10.8 9.4 7.1

    GreenBlood transfusions, parenteral nutrition,stem cell harvesting and cell separation,

    large volumes of fluids

    18G 4.8 4.1 2.7

    Pink Blood transfusions, large volumes of fluids 20G 3.2 2.9 1.9

    Blue

    Blood transfusions, most medications and

    fluids 22G 1.9 1.7 1.1

    YellowMedications, short term infusions, fragile

    veins, children24G 0.8 0.7 0.5

    Approximate Flow Rates (l/hr)

    A uide to choosin the correct catheter for our atient

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    Steps of the cannula Insertion procedure

    Preparation of equipment (Tray)

    Preparation of environment

    Preparation of patient

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    Tourniquet

    Examination Gloves

    Sterile Drapes Surgical Scissors

    Cotton Swabs

    Betadine Swabs

    Spirit Swabs

    5ml / 10ml Syringe Bivalve

    Normal saline flush (10ml)

    Safety cannulas

    Gauze Squares

    Sterile dressing Site label (to record time & date of insertion)

    IV sets (as required)

    IV bottles (as required)

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    Preparation of environment

    Provide privacy

    Well lit room

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    Preparation of patient

    Inform the patient about the procedure

    Site selection Vein should be visible, soft, elastic, straight, palpable

    & without valve (metacarpal ,basilic ,cephalic)

    Observe skin for abrasions, hematoma, local skin

    infection etc. Vein visibility

    a. Clip the selected site if required

    b. Apply tourniquet 6-8 inches above the site

    c. Palpate the selected veind. Instruct the patient to pump his fist 2-3 times

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    Preparation of patient

    Cleaning the site - Clean the chosen areacovering about 2-3 inches radius, with spirit /alcohol, and let it dry. Thereafter betadine solutionis used to clean the same area in circular motionand allow to dry. Do not repalpate or touch the

    cleaned site

    Safety Cannulas should be selected according tothe purpose, vein size and fluid / medication

    requirement.

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    Destroys bacteria by denaturingcell proteins

    Alcohol is fast drying and provides

    immediate kill

    WHY ALCOHOL ALONE IS NOT

    THE BEST PRACTICE

    Residual Ac t iv i ty : None, so

    has no long term

    ant imicrob ial effect iveness

    70% Alcohol

    Combines with proteins of the cellcausing the organism to die

    WHY IODOPHORS ALONE IS NOT

    THE BEST PRACTICE

    Does not provide immediate

    k i l l of m icroorganisms

    Requires minimum 2 minutes of

    skin contact to begin anti-

    microbial effect

    10% Iodophor

    Choosing the right skin preparation

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    Use alcohol followed by application of main

    disinfectant - 10% Povidone Iodine or 2%Chlorhexidine prep.

    Provides immediate kill as well as residual activity

    For Iodophor - 2-3 hrs

    For Chlorhexidine prep. - 6 hrs

    Process - 2 Steps

    1. Apply alcohol in circular motion outwards, allow it todry

    2. Apply Povidone Iodine or Chlorhexidine in circularmotion outwards, allow it to dry

    Best practice for Site Preparation

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    CLSI - Clinical and Laboratory Standards Institute, USA Cleansing the site first with 70% isopropyl alcohol, Allow it to air dry

    Followed by application of the main disinfectant - Povidone Iodine or CHG

    INS (1998, S53) - Infusion Nursing Society, USAAntimicrobial solution containers should be in a single-unit of use and that

    they should be discarded after individual use

    Excess hair over venipuncture site should be clipped instead of shaved

    CDC - Centre for Disease Control and Prevention, USA 2% Chlorhexidine based antimicrobial preparation is preferred*

    Palpation of catheter insertion site should not be performed after

    application of antiseptic*

    Allow the antiseptic to remain on the insertion site and to air dry beforecatheter insertion*

    * CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 (

    No. RR 10 )

    ** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society, INS

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    CLSI - Clinical and Laboratory Standards Institute, USA Cleansing the site first with 70% isopropyl alcohol, Allow it to air dry

    Followed by application of the main disinfectant - Povidone Iodine or CHG

    INS (1998, S53) - Infusion Nursing Society, USAAntimicrobial solution containers should be in a single-unit of use and that

    they should be discarded after individual use

    Excess hair over venipuncture site should be clipped instead of shaved

    CDC - Centre for Disease Control and Prevention, USA 2% Chlorhexidine based antimicrobial preparation is preferred*

    Palpation of catheter insertion site should not be performed after

    application of antiseptic*

    Allow the antiseptic to remain on the insertion site and to air dry beforecatheter insertion*

    * CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 (

    No. RR 10 )

    ** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society, INS

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    CLSI - Clinical and Laboratory Standards Institute, USA Cleansing the site first with 70% isopropyl alcohol, Allow it to air dry

    Followed by application of the main disinfectant - Povidone Iodine or CHG

    INS (1998, S53) - Infusion Nursing Society, USAAntimicrobial solution containers should be in a single-unit of use and that

    they should be discarded after individual use

    Excess hair over venipuncture site should be clipped instead of shaved

    CDC - Centre for Disease Control and Prevention, USA 2% Chlorhexidine based antimicrobial preparation is preferred*

    Palpation of catheter insertion site should not be performed after

    application of antiseptic*

    Allow the antiseptic to remain on the insertion site and to air dry beforecatheter insertion*

    * CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 (

    No. RR 10 )

    ** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society, INS

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    Procedure

    Assemble all articles at patient bedside. Thorough hand washing (Follow 6 steps)

    Wear clean gloves.

    Support the chosen limb.

    Apply the tourniquet.

    Assess and select the vein by gently tapping

    the site.

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    Insertion

    Disinfect the injection site according to local hospital

    policy Remove the catheter from the packaging and lower the

    wings.

    Adopt your preferred grip and remove the needle cover

    Insert the catheter at 15-30 degree angle Upon primary flashback (back flow), lower the angle

    almost parallel to the skin

    Advance the catheter slightly, 2- 3 millimeters, to ensure

    catheter tip is in vein You might consider stabilizing the catheter by holding one

    of the wings

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    Insertion

    Ease the needle back 2- 3 millimeters. Secondary flashback between the needle &

    catheter will confirm correct placement of the

    catheter in the vein.

    Advance the catheter completely into the vein. Remove the tourniquet.

    Stabilize the catheter by holding one wing.

    Occlude the vein just above catheter tip &withdraw the needle holding the needle grip or grip

    plate.

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    Recording

    Upon Insertion of the catheter one should always

    - Record the date of insertion.

    - Record the site of insertion.

    - Record the name of the person who has

    inserted the catheter.- Record the Safety cannula Number

    (Gauge)

    - Signature of the person who has entered

    the catheter.

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    Line Management

    Use aseptic technique at all times.

    All IV ports should be closed. Verify patency of line by gently flushing with normal

    saline.

    Ensure that lines are labeled (date, time, signature)

    Remove line on any sign of redness ,swelling orpain.

    Change IV set after every 24- 48 hrs.

    Cannula to be inspected after every 6- 8 hrs inadults. In Neonates it should be every 2- 4 hrs.

    For giving antibiotics SAS (Saline followed byAntibiotic followed by Saline) must be followed.

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    Line Management

    Use aseptic technique at all times.

    All IV ports should be closed. Verify patency of line by gently flushing with normal

    saline.

    Ensure that lines are labeled (date, time, signature)

    Remove line on any sign of redness ,swelling orpain.

    Change IV set after every 24- 48 hrs.

    Cannula to be inspected after every 6- 8 hrs inadults. In Neonates it should be every 2- 4 hrs.

    For giving antibiotics SAS (Saline followed byAntibiotic followed by Saline) must be followed.

    P t P d

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    Post Procedure

    Remove all equipment and dispose in proper

    manner. Document

    Date and time

    Gauge and length of catheter Site of placement

    The patients response

    Initials.

    Inform the patient about signs and symptoms that

    should be reported such as pain or swelling.

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    Dressings

    Replace dressing when dressing becomesdamp, loosened or visibly soiled.

    Use clean gloves while changing dressing.

    Document dressing changes with date, time.

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    Dressing Guidelines

    Use either sterile gauze or steriletransparent, semi permeabledressing to cover the catheter site

    Replace catheter dressing if the

    dressing becomes damp, loose, orvisibly soiled

    Replace dressings at every 2 daysfor gauze dressing and 7 days fortransparent (TSM) dressing

    CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR,

    2002: 51 ( No. RR 10 )

    Sit C

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    Site Care

    Inspect through transparent dressing on each shift.

    If transparent dressing is not used palpate insertion sitefor pain or tenderness.

    Remove an opaque/ gauze dressing and inspect visually

    if patient develops local tenderness or signs of infection.

    Replace catheter as soon as possible or with in 48 hrs

    when aseptic technique during insertion can not be

    ensured.

    Clean access port with antiseptic and access port only

    with sterile device.

    Cap stop cocks when not in use.

    Si C

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    Site Care

    Document the status of IV access device and the site.

    Flush IV cannula at least once in 8hrs with NormalSaline/heplock if not in use.

    Restart peripheral IV sites every 72-96 hours inadults.

    Peripheral catheter can be retained in pediatricpatients

    until development of any complication.

    If the patient is febrile without another obvious cause

    remove the dressing to usually inspect the site.

    Remove the IV catheter as soon as its use is over.

    Si id

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    Sites to avoid

    Veins in the lower extremities

    Points of flexion Veins close to arteries

    Obvious valves

    Median cubital veins

    Small visible superficial veins Veins irritated from previous use

    Sclerosed vein

    Limbs affected by clinical condition

    Infected sites

    Broken skin

    Ri k d ti th h C th t

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    Risk reduction through Catheter

    Flushing

    C h Fl hi

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    Catheter care - Flushing

    All vascular access devices used should be flushed with 0.9% sodiumchloride (normal saline) or heparin to* Maintain catheter patency Prevent contact between incompatible fluids and medications

    Appropriate Flushing helps to reduce catheter thrombosis and thus CR-BSIrisk** As thrombi or fibrin deposits could serve as a nidus for microbial colonization

    When catheter flushing is to be performed Just after catheter insertion

    Before and after each administration of medication

    Blood sampling

    Every 6-8 hours when catheter is not in use (Once a day - home care PICCs )

    INS standards, 2006 Single use flushing systems to be used, that is, do not use multiple use vials

    ~ 8% Syringes prepared by nurses are contaminated - Syringe tip, Fluid*** Touch contamination

    Multiple use vials or their inappropriate usage

    * Infusion Therapy in clinical practice Judy Hankins et al, 2nd

    Edition, The Infusion Nursing Society,page 394, ** CDC, Centre for Disease Control and Prevention, Guidelines for prevention of IV catheter related Infections,

    MMWR, 2002: 51, Page 9 ( No. RR 10 ), ** APIC, Lynn Hadaway, Webinar series 2006

    C h Fl hi

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    Catheter care - Flushing Heparin lock usually not required in short peripheral catheters

    Heparin, in lowest possible concentration, is the accepted solution for Centralvenous catheters

    When using heparin, use smallest dose possible so that it does not alterpatients coagulation factors

    Use saline flush between medication and heparin, to avoid any incompatibilitywith other IV medications

    Volume for flushing

    Minimum should be twice the internal volume of catheter system (Catheter +

    add on devices ) Follow the hospital policy on volume and concentration

    Maintain positive pressure techniques for flushing

    Close the clamp on the ext. set before disconnecting syringe, Leave the clampclosed before until catheter is used again

    As the last ml of fluid is flushed in, withdraw the syringe

    Catheter should not be flushed to remove an occlusion

    * Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society,page 394, ** CDC, Centre for Disease Control and Prevention, Guidelines for prevention of IV catheter related Infections,

    MMWR, 2002: 51, Page 9 ( No. RR 10 )

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    Appropriate Usage of

    Equipments

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    Appropriate use of equipmentIntravasular Access

    Monitor and inspect catheter site regularly, the site should

    be observed for any signs of inflammation, infection ormalfunction

    Use vented IV sets with plastic non collapsible IV bags orglass bottles

    Non vented IV set can only be used with collapsibleplastic bags**

    Use single dose Vials for parenteral additives or anymedications

    If multi-dose Vials are used, cleanse the accessdiaphragm of the multi-dose vial with 70% alcoholbefore inserting a device into the vial

    CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 ( No. RR 10 )

    ** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society,

    a e 394

    Appropriate se of eq ipment

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    Appropriate use of equipment

    For any intravascular access

    Replace IV tubing and add on devices no more frequently than72 hours

    Replace tubing used to administer blood products or lipids within 24 hrs.

    Clean injection ports with 70% alcohol or an iodophor beforeaccessing

    IVD replacement

    Peripheral Venous : 72-96 hrs. in adults / first signs of phlebitis,

    In pediatric patients, Do not routinely replace peripheralvenous catheters unless clinically indicated

    CVCs / PICC / Hemodialysis / PA / Peripheral Arterial : NOT

    routinely*

    CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 ( No. RR 10 )

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    Closed Port for infection

    prevention

    Open Ports

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    Open Ports CDC - Centre for Disease Control and Prevention*

    Stopcocks represent a potential portal of entry formicroorganisms into vascular access

    Stopcock contamination is common, occurring in45-50% cases

    INS - Infusion Nursing Society, USA**

    Studies have shown stopcocks have often beencause of microorganisms entering the IV systemthrough

    hands of personnel,

    syringes used to flush or draw blood

    residual blood that remains in the port afteruse, serving as breeding ground for bacteria

    Failure to keep a sterile cap on when not inuse

    * CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 ( No. RR 10 )

    ** Infusion Therapy in Clinical Practice, Infusion Nurses Society, 2nd Edition, Judy Hankins, et al, chapter 24, Page 429, Pearson ML : Guidelines for prevention of IV device related

    infections, Infect Control Hosp Epidemiol.17(7):438,1996

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    Closed port

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    Closed port

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    Venipuncture &

    Blood Collection

    Where do the laboratory errors happen?

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    Where do the laboratory errors happen?

    St i Bl d C ll ti

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    Steps in Blood Collection

    Patient

    interaction

    Standard

    precautions

    Selecting

    equipment

    Positioning

    the patient

    Tourniquet

    application

    Site

    selection

    Site

    cleansing

    Perform

    venipuncture

    Sample

    handling /

    mixing

    Sharps

    disposalSample

    transport

    Patient

    requisition

    A Laboratory TEST is no better than the SPECIMEN, & the

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    A Laboratory TEST is no better than the SPECIMEN, & the

    specimen no better than the manner in which it was collected

    Hemolysis Fibrin Mass

    EDTA Under fillRed cells in

    suspension

    Fibrin

    threads& poor

    barrier

    formation

    Sample Quality is a challenge

    e -

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    e beyond the control of phlebotomists

    Age

    Race

    Gender

    Pregnancy

    Diet

    Exercise

    Environment/Lifestyle

    These are important variables but not of particular relevance

    to phlebotomists and laboratory staff as physicians will

    generally take these factors into account when interpreting

    test result data

    The PRE ANALYTICAL PHASE

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    The PRE-ANALYTICAL PHASE

    within control of phlebotomist / lab worker

    Phlebotomy Related Causes

    Patient and Specimen Identification

    Dietary Status, Medications

    Collection time (interval from last

    meal, timing in context of drug

    administration and other therapies -

    eg dialysis, transfusion, surgery

    Site of Phlebotomy

    Tourniquet (placement, duration)

    Cleansing of the site

    Quality of Phlebotomy (trauma,

    duration, needle gauge etc.)

    Transport Related Causes

    Improper handling

    Temperature & Humidity

    Time

    Specimen Integrity

    Exposure to LightProcessing Related Causes

    Time

    Centrifugation

    Temperature Storage

    re erre a r u es o ve ns or

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    Large enough to support good flow

    Easily visible

    Close to the skin surface

    Elastic do not feel too hard

    Well anchored in surrounding tissue

    re erre a r u es o ve ns or venipuncture

    Site selection in Arm

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    Site selection in Arm

    1. Median cubital vein

    This is the first choice because

    It is large Well-anchored

    Generally least painful

    Least likely to bruise

    2. Cephalic vein

    This is the second choice

    It is large

    Not as well-anchored

    May be more painful than the median cubital vein

    3. Basilic vein

    This is the third choice

    It is generally large It is easy to palpate (elastic / feel)

    Often not well anchored (slippery)

    1. It lies near brachial artery and median nerve either of whichcould be accidentally punctured

    Inappropriate Sites for Venipuncture

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    Inappropriate Sites for Venipuncture

    Arms on side of mastectomy

    Edematous areas

    Hematomas

    Scarred areas

    Burns

    Tattoos

    Damaged veins (e.g thrombosed, non-elastic veins)

    Sites downstream (proximal) from an IV line*

    * Note that whilst it is always preferable to draw blood from the opposite arm in this situation,phlebotomy may be performed upstream (distal) on the same arm when a suitable site is not

    available on the opposite arm. Where there is no alternative to drawing downstream (on the same

    arm), the line may be switched off** and the specimen collected after a minimum wait of 2minutes. These specimens must be labeled accordingly (e.g. intra-transfusion) (Ref: CLSI H03-

    A6 Procedures for the Collection of Diagnostic Blood Specimens by venipuncture; Approved

    Standard - Fifth Edition, p. 23, paragraph 11.5.1)

    ** with permission of the doctor in charge of the case!

    E i t d S li

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    Tourniquet

    GlovesAntiseptic & Cotton

    Needle

    Syringe or Needle

    holder

    Specimen

    Container

    Gauze Tape or Bandage

    Sharps Container

    Equipment and Supplies

    Patient Identification

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    Patient IdentificationThis is the most important step in the venipunctureprocedure*

    Out-patients:

    Patient to be asked to state his/her fullname, spell the last name and date of birth

    This is verified with the information on therequisition

    In-Patients:

    The patient's identification band to bechecked in order to verify the name andhospital identification number and match theorder.

    The patients ID to be verified with ward staffif identification band is not available

    Young or Mentally incompetent patient: May ask patients nurse, attendant, relative

    to identify him / her

    * patient ID error is the ultimate pre-analytical error!

    Tourniquets

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    Tourniquets

    Stretchable strip of material 35 to 45

    cm (15 to 18 inches) in length and maybe single-use or re-usable.

    Different types of tourniquets are:

    Latex

    Vinyl usefulwhere the healthcare

    workeror patient is allergic to latex

    Elastic band with Velcro or buckleclosure

    Use of a Tourniquet

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    Use of a Tourniquet

    Makes the veins easier to locate and feel

    Slows down venous flow

    Enlarges the veins

    Should not restrict arterial blood flow into the limb

    Wrap 7.5 to 10.0 cm ( 3 - 4 inches) above intended

    venipuncture site

    Tied to be releasable with one hand

    Tourniquet time = maximum 1 minute

    Gloves

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    GlovesKey element of standard

    infection control precautionsProvide a barrier to spread of

    infection

    Part of personal protectiveequipment (PPE) against contact

    with blood during phlebotomy

    Good fit is essential

    Washing or reuse of gloves can compromise integrity of material to serve as barrierwithout visible changes.

    Gauze Pads & Bandages

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    Gauze Pads & Bandages

    Gauze Pads

    Should be clean

    Used to apply pressure on site after

    needle removal

    Adhesive Bandages/Tape

    Used to secure gauze

    Do not apply directly on the site

    Cotton not recommended as fibers can stick to site and initiate bleeding

    when removed Do not use alcohol swab

    Antiseptic and Disinfectant

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    Antiseptic and DisinfectantAntiseptic

    Inhibit or prevent the growth of bacteria

    Approved for used on the skin

    Used to clean venipuncture site

    70% isopropyl alcoholmost commonly used

    Disinfectant

    Kills bacteria and inhibits some viruses

    Check manufactures label

    For use on surfaces and instruments, not on skin

    Used to clean up all blood spills

    1:10 hypochlorite (bleach) solution commonly used

    Isopropyl alcohol

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    70% optimal concentration asantiseptic

    Store in closed container

    Active ingredient evaporates fromopen container leaving water behind

    Antiseptic properties diminished Bacteria from hands, container can

    multiply and cause infection inpatient if used to clean intendedpuncture site

    Cleaned site may not dry quickly

    Isopropyl alcohol

    Never pre-soak cotton balls

    Cleaning the Site

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    Cleaning the Site

    Use pre-packaged swab or cotton ballthat has been moistened with antiseptic

    at time of use on patient

    Clean area in a spiral starting at theintended site of puncture moving

    outward

    Let air dry

    Do not blow or fan site

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    Clinical And Laboratory Standards Institute

    Syringe and Needle Blood Collection

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    Syringe

    Variety of sizes:

    2ml, 5ml,10ml, 20 ml Selection depends on

    Patient

    Volume of blood to collect

    Strength of vacuum created

    Advantage

    Blood flash can be seen on

    entering vein

    Disadvantage

    Specimen can clot

    Specimen must be transferred

    Never exert pressure on plunger in

    transfer to vacuum tube

    Vacuum to draw blood

    from vein throughneedle and into syringe

    created as plunger

    withdrawn

    User controls thevacuum

    Syringe and Needle Blood Collection

    Venipuncture Procedure Using Needle

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    p gand Syringe

    After needle is in the vein,slowly pull the plunger back

    to start filling the syringe.

    While blood flows into the

    syringe, release the

    tourniquet.

    Transfer of Blood From Syringe into

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    Remove the cap of the container

    and gently transfer specimen

    into it by pushing the plunger

    Ensure there is no froth

    formation during the blood flowinto the container

    Do not overfill

    Replace the container cap

    y gSpecimen Container

    Transfer of Blood from a Syringe into a

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    Do not remove rubber stopper when using

    evacuated tubes. Place the tube upright in a rack.

    Slowly pierce the stopper of the evacuated tube

    with the needle.

    Allow the tube to fill (without applying pressure

    to the plunger) until blood flow into the tube

    ceases. This technique helps to maintain the

    correct ratio of blood to additive.

    Follow the same order for filling tubes as the

    order of draw for an evacuated system.

    y g

    Evacuated Tube

    1. WHO guidelines for drawing blood: best practices in phlebotomy, November 2009

    Evacuated Closed Blood Collection

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    Evacuated Closed Blood Collection

    Vacuum in tube allowsblood to be drawn directly

    from vein into evacuated

    tube

    No need to transfer blood

    Tube Filling evacuated system

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    g y Maintain downward position so blood and additive do not

    touch non-patient end of multi-sample needle.

    Allow to fill until vacuum is exhausted and flow stops. Remove from holder by applying pressure against wings*

    of holder with thumb and index finger. This assists inholding needle steady as tubes are removed and inserted.

    Invert gently several times after removal to mix blood andadditive. Additional mixing per tube type can be performedwhile next tubes are filling.

    Continue to draw tubes following correct order of draw.

    * As for tube placement above, this is critical to thesuccess of the procedure.

    Order of Draw

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    Order of Draw

    (with clot activator)

    1. Sterile samples (eg: Blood Cultures)

    2. Citrate tubes*

    3. Plain Serum tubes

    4. Heparin tubes

    5. EDTA tubes

    6. Fluoride Oxalate (glucose tubes)

    7. ACD tubes

    * this tube is acceptable for routine coagulation testing (e.g. aPTT and PT/INR). For some special coagulation testing where low level activation of coagulation

    factors may be of particular concern**, the use of a non-additive discard tube may be considered1. Because of dead space in the tubing of winged (butterfly)

    sets, a discard tube should also be used where citrate tubes are the first drawn and a winged collection set is used. This is particulally important when 1.8mL and

    2.7mL tubes are used.

    ** activation may be induced by tissue factor (tissue thromboplastin) introduced into the initial sample stream as the needle traverses the subcutaneous tissue.

    Note regarding ESR: in accordance with CLSI1 recommendations, ESR tubes should be placed at position 2 above.

    1. CLSI document H3-A6 Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture. 6th Edition, 2007

    Mixing of Tubes

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    Why

    All tubes contain

    additive that needs to be

    mixed with the blood

    sample.

    Tubes withanticoagulants such as

    EDTA need to be mixed

    to ensure the specimen

    does not clot.

    How

    Holding tube

    upright, gently

    invert 180 andback.

    When Immediately after

    drawing.

    Consequences if not mixed:

    Tubes with anticoagulants will clot

    Specimen will often need to be redrawn

    Mixing of Tubes

    Needle Disposal

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    Needle Disposal Ifa single-use holder is used, the complete assembly should

    bediscarded into a puncture-proof sharps container.

    Otherwise - The contaminated needles must be safely removed from the

    holder and discarded in a suitable sharps container1,2

    Nevercut, bend, break, burn, or re-cap needles.

    A variety of containers is available. Most have a facility3to

    provide safe removal of the needle from the (re-usable) holderwhen using an evacuated tube system.

    1. Needles must not be removed with fingers. If needle doesnot separate from the holder, the entire assembly may bediscarded in the sharps container.

    2. Place needle in the proper slot in the lid, and rotate anti-

    clockwise until it unscrews from the holder. Allow theneedle to drop into the container

    Sharpcontainers should not be overfilled

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    Post venipuncture

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    p

    The patients arm is examined to see if bleeding hasstopped.

    An adhesive bandage or tape is applied on the site(following institutional policies).

    The patient is instructed to leave the bandage on for aminimum of 15 minutes.

    Out patients should be advised not to carry a purse or otherheavy object or lift heavy objects with that arm for 1 hour.

    The patient should be thanked for his or her cooperationthis helps leave the patient with a positive feeling.*

    (Contaminated materials should be disposed of in approvedbiohazard containers following the institutional policiesbefore attending to the next patient.

    *Reference: Ruth E McCall & Cathee M. Tankersley. "Phlebotomy Essentials" 3rd edition (2003) p 272.

    Tube Transportation

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    p Tubes transported through public hallways should be placed in a

    secondary container to minimize the risk of leakage and spillage.

    The secondary container shouldbe clearly labeled "BIOHAZARD.

    Once the tubes are in the container, they should be sealed prior totransport.

    Once primary containers are inside the externally uncontaminatedsecondary container, they can be handled without gloves2.

    Personnel who transport specimens should be trained in safe handlingpractices and decontamination procedures2.

    Paper requisition(s) and other documents (if present) mustbe attachedsecurely to the secondary transport container.

    All necessary steps shouldbe taken to avoid exposure of tubes tomechanical trauma, temperature extremes and delays duringtransportation as these can introduce significant pre-analytical error1.

    Once received in the laboratory, gloves should be worn while removingspecimens from the secondary container and for all manipulations ofprimary container2.

    1. CLSI Document H18-A3. Procedures for the Handling and Processing of Blood Specimens; Approved Guideline, 3rdEdition, 2004

    2. CLSI Document M29-A3 Protection of Laboratory workers from occupationally acquired infections; Approved Guidelines,3rd Edition, 2005

    Special Collection Procedures

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    p Use of winged collection set is recommended for all blood

    culture collection procedures.

    Thorough skin preparation is essential* The site should first be cleansed with 70% alcohol

    The site should then be swabbed with 1-10% povidine-iodine solution or chlorhexidine gluconate (the latter isrecommended for infants greater than 2 months of ageand patients with iodine sensitivity) by circular motion,

    starting in the middle. The site must then be allowed to dry. The iodine /

    chlorhexidine can then be removed with an alcoholswab.

    * institutional policies and procedures must befollowed

    The culture bottle stopper should be disinfected followingmanufacturers instructions.

    Summary

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    y

    Prepare the tray. Ensure all the logistic/equipment areavailable in the tray

    Choose the correct site for cannulation.Avoidjoint/obliterated vein

    Follow aseptic technique, wash hand,clean cannulationsite and wear clean gloves

    Maintain record of cannulation

    Watch for complication like phlebitis/haematoma etc.

    Replace cannula every 72-96 hours

    Dispose sharps and other materials appropriately as per

    guideline(CPCP/SPCB)