Module 2 - Infusion Safety
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Transcript of Module 2 - Infusion Safety
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Protocol For Inserting A Peripheral Line
Only staff nurses/trained medical professionalswith assistance will insert a peripheral cannula.
All peripheral lines should be replaced in 72
hrs. (96 hrs if vialon material used)
Sterile dressing should be reinforced as and
when necessary.
Make a record of person inserting peripheral
line, including time and date of insertion.
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Cannulating staff nurses and head nurses should
know infusion therapy process model. Anatomy & Physiology of the part to be
cannulated should be known
Patient consideration (thin, fat, hydration status)
Therapy consideration Initiation of therapy
Dressing
After care of catheter
Managing complications
Protocol For Inserting A Peripheral Line
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1. Successful completion
of infusion therapy
2. Reduced catheter
related complications
3. Minimized number of
venipunctures per
patient
4. Reduced supplyrelated costs
5. Reduced labor related
costs
6. Patient satisfaction
7. Reduced needlestick
injuries
Outcomes
Patient
Considerations
Device
Selection
Site
Preparation
Site
Maintenance
Site Assessment
& EvaluationTherapy
Considerations
Clinician
ConsiderationsInsertion
Considerations
Purpose
Administration Rate
Duration
Nature of Fluids/Meds Insertion Difficulty
Access Limitations
Vein
Skin Status
Interventional Radiologist
Insertion Angle
Insertion Speed
Skin Traction
Vein Dilation
Safety Practices
Needle Disposal
Catheter Advancement
Infection Control Practices
Education Needs
Disease State
Orientation
Care Setting
MobilityAge
Therapy Considerations
Risk Assessment
Device Material
Safety Features
Cost-in-Use
Patient Preferences
Antimicrobial Awareness
Appropriate Application
Local AnestheticHair Removal Asepsis
Documentation
Flushing Protocols
Monitor/Assessment
Dressing Integrity
Administration Set Protocols
Maintenance Protocols
Catheter Stabilization
Delivery Equipment Requirements
Types
Allergies
Gauge & Length
Complication Prevention
BD DECISIV
Process Model-
for Safe InfusionSpecialized Areas
Generalist
Physician
IV Specialist
Copyright 1997
Updated: March
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1. Successful completion
of infusion therapy
2. Reduced catheter
related complications
3. Minimized number of
venipunctures per
patient
4. Reduced supplyrelated costs
5. Reduced labor related
costs
6. Patient satisfaction
7. Reduced needlestick
injuries
Outcomes
Patient
Considerations
Device
Selection
Site
Preparation
Site
Maintenance
Site Assessment
& EvaluationTherapy
Considerations
Clinician
ConsiderationsInsertion
Considerations
Purpose
Administration Rate
Duration
Nature of Fluids/Meds Insertion Difficulty
Access Limitations
Vein
Skin Status
Interventional Radiologist
Insertion Angle
Insertion Speed
Skin Traction
Vein Dilation
Safety Practices
Needle Disposal
Catheter Advancement
Infection Control Practices
Education Needs
Disease State
Orientation
Care Setting
MobilityAge
Therapy Considerations
Risk Assessment
Device Material
Safety Features
Cost-in-Use
Patient Preferences
Antimicrobial Awareness
Appropriate Application
Local AnestheticHair Removal Asepsis
Documentation
Flushing Protocols
Monitor/Assessment
Dressing Integrity
Administration Set Protocols
Maintenance Protocols
Catheter Stabilization
Delivery Equipment Requirements
Types
Allergies
Gauge & Length
Complication Prevention
BD DECISIV
Process Model-
for Safe InfusionSpecialized Areas
Generalist
Physician
IV Specialist
Copyright 1997
Updated: March
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Choose correct catheter
COLOUR COMMON APPLICATIONS SIZE/GAUGE CRYSTALLOID PLASMA BLOOD SANG
OrangeUsed in theatres or emergency for rapid
transfusion of blood or viscous fluids14G 16.2 13.5 10.3
GreyUsed in theatres or emergency for rapid
transfusion of blood or viscous fluids16G 10.8 9.4 7.1
GreenBlood transfusions, parenteral nutrition,stem cell harvesting and cell separation,
large volumes of fluids
18G 4.8 4.1 2.7
Pink Blood transfusions, large volumes of fluids 20G 3.2 2.9 1.9
Blue
Blood transfusions, most medications and
fluids 22G 1.9 1.7 1.1
YellowMedications, short term infusions, fragile
veins, children24G 0.8 0.7 0.5
Approximate Flow Rates (l/hr)
A uide to choosin the correct catheter for our atient
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Steps of the cannula Insertion procedure
Preparation of equipment (Tray)
Preparation of environment
Preparation of patient
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Tourniquet
Examination Gloves
Sterile Drapes Surgical Scissors
Cotton Swabs
Betadine Swabs
Spirit Swabs
5ml / 10ml Syringe Bivalve
Normal saline flush (10ml)
Safety cannulas
Gauze Squares
Sterile dressing Site label (to record time & date of insertion)
IV sets (as required)
IV bottles (as required)
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Preparation of environment
Provide privacy
Well lit room
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Preparation of patient
Inform the patient about the procedure
Site selection Vein should be visible, soft, elastic, straight, palpable
& without valve (metacarpal ,basilic ,cephalic)
Observe skin for abrasions, hematoma, local skin
infection etc. Vein visibility
a. Clip the selected site if required
b. Apply tourniquet 6-8 inches above the site
c. Palpate the selected veind. Instruct the patient to pump his fist 2-3 times
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Preparation of patient
Cleaning the site - Clean the chosen areacovering about 2-3 inches radius, with spirit /alcohol, and let it dry. Thereafter betadine solutionis used to clean the same area in circular motionand allow to dry. Do not repalpate or touch the
cleaned site
Safety Cannulas should be selected according tothe purpose, vein size and fluid / medication
requirement.
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Destroys bacteria by denaturingcell proteins
Alcohol is fast drying and provides
immediate kill
WHY ALCOHOL ALONE IS NOT
THE BEST PRACTICE
Residual Ac t iv i ty : None, so
has no long term
ant imicrob ial effect iveness
70% Alcohol
Combines with proteins of the cellcausing the organism to die
WHY IODOPHORS ALONE IS NOT
THE BEST PRACTICE
Does not provide immediate
k i l l of m icroorganisms
Requires minimum 2 minutes of
skin contact to begin anti-
microbial effect
10% Iodophor
Choosing the right skin preparation
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Use alcohol followed by application of main
disinfectant - 10% Povidone Iodine or 2%Chlorhexidine prep.
Provides immediate kill as well as residual activity
For Iodophor - 2-3 hrs
For Chlorhexidine prep. - 6 hrs
Process - 2 Steps
1. Apply alcohol in circular motion outwards, allow it todry
2. Apply Povidone Iodine or Chlorhexidine in circularmotion outwards, allow it to dry
Best practice for Site Preparation
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CLSI - Clinical and Laboratory Standards Institute, USA Cleansing the site first with 70% isopropyl alcohol, Allow it to air dry
Followed by application of the main disinfectant - Povidone Iodine or CHG
INS (1998, S53) - Infusion Nursing Society, USAAntimicrobial solution containers should be in a single-unit of use and that
they should be discarded after individual use
Excess hair over venipuncture site should be clipped instead of shaved
CDC - Centre for Disease Control and Prevention, USA 2% Chlorhexidine based antimicrobial preparation is preferred*
Palpation of catheter insertion site should not be performed after
application of antiseptic*
Allow the antiseptic to remain on the insertion site and to air dry beforecatheter insertion*
* CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 (
No. RR 10 )
** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society, INS
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CLSI - Clinical and Laboratory Standards Institute, USA Cleansing the site first with 70% isopropyl alcohol, Allow it to air dry
Followed by application of the main disinfectant - Povidone Iodine or CHG
INS (1998, S53) - Infusion Nursing Society, USAAntimicrobial solution containers should be in a single-unit of use and that
they should be discarded after individual use
Excess hair over venipuncture site should be clipped instead of shaved
CDC - Centre for Disease Control and Prevention, USA 2% Chlorhexidine based antimicrobial preparation is preferred*
Palpation of catheter insertion site should not be performed after
application of antiseptic*
Allow the antiseptic to remain on the insertion site and to air dry beforecatheter insertion*
* CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 (
No. RR 10 )
** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society, INS
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CLSI - Clinical and Laboratory Standards Institute, USA Cleansing the site first with 70% isopropyl alcohol, Allow it to air dry
Followed by application of the main disinfectant - Povidone Iodine or CHG
INS (1998, S53) - Infusion Nursing Society, USAAntimicrobial solution containers should be in a single-unit of use and that
they should be discarded after individual use
Excess hair over venipuncture site should be clipped instead of shaved
CDC - Centre for Disease Control and Prevention, USA 2% Chlorhexidine based antimicrobial preparation is preferred*
Palpation of catheter insertion site should not be performed after
application of antiseptic*
Allow the antiseptic to remain on the insertion site and to air dry beforecatheter insertion*
* CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 (
No. RR 10 )
** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society, INS
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Procedure
Assemble all articles at patient bedside. Thorough hand washing (Follow 6 steps)
Wear clean gloves.
Support the chosen limb.
Apply the tourniquet.
Assess and select the vein by gently tapping
the site.
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Insertion
Disinfect the injection site according to local hospital
policy Remove the catheter from the packaging and lower the
wings.
Adopt your preferred grip and remove the needle cover
Insert the catheter at 15-30 degree angle Upon primary flashback (back flow), lower the angle
almost parallel to the skin
Advance the catheter slightly, 2- 3 millimeters, to ensure
catheter tip is in vein You might consider stabilizing the catheter by holding one
of the wings
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Insertion
Ease the needle back 2- 3 millimeters. Secondary flashback between the needle &
catheter will confirm correct placement of the
catheter in the vein.
Advance the catheter completely into the vein. Remove the tourniquet.
Stabilize the catheter by holding one wing.
Occlude the vein just above catheter tip &withdraw the needle holding the needle grip or grip
plate.
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Recording
Upon Insertion of the catheter one should always
- Record the date of insertion.
- Record the site of insertion.
- Record the name of the person who has
inserted the catheter.- Record the Safety cannula Number
(Gauge)
- Signature of the person who has entered
the catheter.
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Line Management
Use aseptic technique at all times.
All IV ports should be closed. Verify patency of line by gently flushing with normal
saline.
Ensure that lines are labeled (date, time, signature)
Remove line on any sign of redness ,swelling orpain.
Change IV set after every 24- 48 hrs.
Cannula to be inspected after every 6- 8 hrs inadults. In Neonates it should be every 2- 4 hrs.
For giving antibiotics SAS (Saline followed byAntibiotic followed by Saline) must be followed.
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Line Management
Use aseptic technique at all times.
All IV ports should be closed. Verify patency of line by gently flushing with normal
saline.
Ensure that lines are labeled (date, time, signature)
Remove line on any sign of redness ,swelling orpain.
Change IV set after every 24- 48 hrs.
Cannula to be inspected after every 6- 8 hrs inadults. In Neonates it should be every 2- 4 hrs.
For giving antibiotics SAS (Saline followed byAntibiotic followed by Saline) must be followed.
P t P d
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Post Procedure
Remove all equipment and dispose in proper
manner. Document
Date and time
Gauge and length of catheter Site of placement
The patients response
Initials.
Inform the patient about signs and symptoms that
should be reported such as pain or swelling.
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Dressings
Replace dressing when dressing becomesdamp, loosened or visibly soiled.
Use clean gloves while changing dressing.
Document dressing changes with date, time.
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Dressing Guidelines
Use either sterile gauze or steriletransparent, semi permeabledressing to cover the catheter site
Replace catheter dressing if the
dressing becomes damp, loose, orvisibly soiled
Replace dressings at every 2 daysfor gauze dressing and 7 days fortransparent (TSM) dressing
CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR,
2002: 51 ( No. RR 10 )
Sit C
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Site Care
Inspect through transparent dressing on each shift.
If transparent dressing is not used palpate insertion sitefor pain or tenderness.
Remove an opaque/ gauze dressing and inspect visually
if patient develops local tenderness or signs of infection.
Replace catheter as soon as possible or with in 48 hrs
when aseptic technique during insertion can not be
ensured.
Clean access port with antiseptic and access port only
with sterile device.
Cap stop cocks when not in use.
Si C
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Site Care
Document the status of IV access device and the site.
Flush IV cannula at least once in 8hrs with NormalSaline/heplock if not in use.
Restart peripheral IV sites every 72-96 hours inadults.
Peripheral catheter can be retained in pediatricpatients
until development of any complication.
If the patient is febrile without another obvious cause
remove the dressing to usually inspect the site.
Remove the IV catheter as soon as its use is over.
Si id
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Sites to avoid
Veins in the lower extremities
Points of flexion Veins close to arteries
Obvious valves
Median cubital veins
Small visible superficial veins Veins irritated from previous use
Sclerosed vein
Limbs affected by clinical condition
Infected sites
Broken skin
Ri k d ti th h C th t
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Risk reduction through Catheter
Flushing
C h Fl hi
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Catheter care - Flushing
All vascular access devices used should be flushed with 0.9% sodiumchloride (normal saline) or heparin to* Maintain catheter patency Prevent contact between incompatible fluids and medications
Appropriate Flushing helps to reduce catheter thrombosis and thus CR-BSIrisk** As thrombi or fibrin deposits could serve as a nidus for microbial colonization
When catheter flushing is to be performed Just after catheter insertion
Before and after each administration of medication
Blood sampling
Every 6-8 hours when catheter is not in use (Once a day - home care PICCs )
INS standards, 2006 Single use flushing systems to be used, that is, do not use multiple use vials
~ 8% Syringes prepared by nurses are contaminated - Syringe tip, Fluid*** Touch contamination
Multiple use vials or their inappropriate usage
* Infusion Therapy in clinical practice Judy Hankins et al, 2nd
Edition, The Infusion Nursing Society,page 394, ** CDC, Centre for Disease Control and Prevention, Guidelines for prevention of IV catheter related Infections,
MMWR, 2002: 51, Page 9 ( No. RR 10 ), ** APIC, Lynn Hadaway, Webinar series 2006
C h Fl hi
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Catheter care - Flushing Heparin lock usually not required in short peripheral catheters
Heparin, in lowest possible concentration, is the accepted solution for Centralvenous catheters
When using heparin, use smallest dose possible so that it does not alterpatients coagulation factors
Use saline flush between medication and heparin, to avoid any incompatibilitywith other IV medications
Volume for flushing
Minimum should be twice the internal volume of catheter system (Catheter +
add on devices ) Follow the hospital policy on volume and concentration
Maintain positive pressure techniques for flushing
Close the clamp on the ext. set before disconnecting syringe, Leave the clampclosed before until catheter is used again
As the last ml of fluid is flushed in, withdraw the syringe
Catheter should not be flushed to remove an occlusion
* Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society,page 394, ** CDC, Centre for Disease Control and Prevention, Guidelines for prevention of IV catheter related Infections,
MMWR, 2002: 51, Page 9 ( No. RR 10 )
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Appropriate Usage of
Equipments
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Appropriate use of equipmentIntravasular Access
Monitor and inspect catheter site regularly, the site should
be observed for any signs of inflammation, infection ormalfunction
Use vented IV sets with plastic non collapsible IV bags orglass bottles
Non vented IV set can only be used with collapsibleplastic bags**
Use single dose Vials for parenteral additives or anymedications
If multi-dose Vials are used, cleanse the accessdiaphragm of the multi-dose vial with 70% alcoholbefore inserting a device into the vial
CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 ( No. RR 10 )
** Infusion Therapy in clinical practice Judy Hankins et al, 2nd Edition, The Infusion Nursing Society,
a e 394
Appropriate se of eq ipment
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Appropriate use of equipment
For any intravascular access
Replace IV tubing and add on devices no more frequently than72 hours
Replace tubing used to administer blood products or lipids within 24 hrs.
Clean injection ports with 70% alcohol or an iodophor beforeaccessing
IVD replacement
Peripheral Venous : 72-96 hrs. in adults / first signs of phlebitis,
In pediatric patients, Do not routinely replace peripheralvenous catheters unless clinically indicated
CVCs / PICC / Hemodialysis / PA / Peripheral Arterial : NOT
routinely*
CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 ( No. RR 10 )
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Closed Port for infection
prevention
Open Ports
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Open Ports CDC - Centre for Disease Control and Prevention*
Stopcocks represent a potential portal of entry formicroorganisms into vascular access
Stopcock contamination is common, occurring in45-50% cases
INS - Infusion Nursing Society, USA**
Studies have shown stopcocks have often beencause of microorganisms entering the IV systemthrough
hands of personnel,
syringes used to flush or draw blood
residual blood that remains in the port afteruse, serving as breeding ground for bacteria
Failure to keep a sterile cap on when not inuse
* CDC, Centre for Disease Control and Prevention, Guidelines for prevention of Intravascular catheter related Infections, MMWR, 2002: 51 ( No. RR 10 )
** Infusion Therapy in Clinical Practice, Infusion Nurses Society, 2nd Edition, Judy Hankins, et al, chapter 24, Page 429, Pearson ML : Guidelines for prevention of IV device related
infections, Infect Control Hosp Epidemiol.17(7):438,1996
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Closed port
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Closed port
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Venipuncture &
Blood Collection
Where do the laboratory errors happen?
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Where do the laboratory errors happen?
St i Bl d C ll ti
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Steps in Blood Collection
Patient
interaction
Standard
precautions
Selecting
equipment
Positioning
the patient
Tourniquet
application
Site
selection
Site
cleansing
Perform
venipuncture
Sample
handling /
mixing
Sharps
disposalSample
transport
Patient
requisition
A Laboratory TEST is no better than the SPECIMEN, & the
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A Laboratory TEST is no better than the SPECIMEN, & the
specimen no better than the manner in which it was collected
Hemolysis Fibrin Mass
EDTA Under fillRed cells in
suspension
Fibrin
threads& poor
barrier
formation
Sample Quality is a challenge
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e beyond the control of phlebotomists
Age
Race
Gender
Pregnancy
Diet
Exercise
Environment/Lifestyle
These are important variables but not of particular relevance
to phlebotomists and laboratory staff as physicians will
generally take these factors into account when interpreting
test result data
The PRE ANALYTICAL PHASE
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The PRE-ANALYTICAL PHASE
within control of phlebotomist / lab worker
Phlebotomy Related Causes
Patient and Specimen Identification
Dietary Status, Medications
Collection time (interval from last
meal, timing in context of drug
administration and other therapies -
eg dialysis, transfusion, surgery
Site of Phlebotomy
Tourniquet (placement, duration)
Cleansing of the site
Quality of Phlebotomy (trauma,
duration, needle gauge etc.)
Transport Related Causes
Improper handling
Temperature & Humidity
Time
Specimen Integrity
Exposure to LightProcessing Related Causes
Time
Centrifugation
Temperature Storage
re erre a r u es o ve ns or
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Large enough to support good flow
Easily visible
Close to the skin surface
Elastic do not feel too hard
Well anchored in surrounding tissue
re erre a r u es o ve ns or venipuncture
Site selection in Arm
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Site selection in Arm
1. Median cubital vein
This is the first choice because
It is large Well-anchored
Generally least painful
Least likely to bruise
2. Cephalic vein
This is the second choice
It is large
Not as well-anchored
May be more painful than the median cubital vein
3. Basilic vein
This is the third choice
It is generally large It is easy to palpate (elastic / feel)
Often not well anchored (slippery)
1. It lies near brachial artery and median nerve either of whichcould be accidentally punctured
Inappropriate Sites for Venipuncture
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Inappropriate Sites for Venipuncture
Arms on side of mastectomy
Edematous areas
Hematomas
Scarred areas
Burns
Tattoos
Damaged veins (e.g thrombosed, non-elastic veins)
Sites downstream (proximal) from an IV line*
* Note that whilst it is always preferable to draw blood from the opposite arm in this situation,phlebotomy may be performed upstream (distal) on the same arm when a suitable site is not
available on the opposite arm. Where there is no alternative to drawing downstream (on the same
arm), the line may be switched off** and the specimen collected after a minimum wait of 2minutes. These specimens must be labeled accordingly (e.g. intra-transfusion) (Ref: CLSI H03-
A6 Procedures for the Collection of Diagnostic Blood Specimens by venipuncture; Approved
Standard - Fifth Edition, p. 23, paragraph 11.5.1)
** with permission of the doctor in charge of the case!
E i t d S li
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Tourniquet
GlovesAntiseptic & Cotton
Needle
Syringe or Needle
holder
Specimen
Container
Gauze Tape or Bandage
Sharps Container
Equipment and Supplies
Patient Identification
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Patient IdentificationThis is the most important step in the venipunctureprocedure*
Out-patients:
Patient to be asked to state his/her fullname, spell the last name and date of birth
This is verified with the information on therequisition
In-Patients:
The patient's identification band to bechecked in order to verify the name andhospital identification number and match theorder.
The patients ID to be verified with ward staffif identification band is not available
Young or Mentally incompetent patient: May ask patients nurse, attendant, relative
to identify him / her
* patient ID error is the ultimate pre-analytical error!
Tourniquets
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Tourniquets
Stretchable strip of material 35 to 45
cm (15 to 18 inches) in length and maybe single-use or re-usable.
Different types of tourniquets are:
Latex
Vinyl usefulwhere the healthcare
workeror patient is allergic to latex
Elastic band with Velcro or buckleclosure
Use of a Tourniquet
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Use of a Tourniquet
Makes the veins easier to locate and feel
Slows down venous flow
Enlarges the veins
Should not restrict arterial blood flow into the limb
Wrap 7.5 to 10.0 cm ( 3 - 4 inches) above intended
venipuncture site
Tied to be releasable with one hand
Tourniquet time = maximum 1 minute
Gloves
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GlovesKey element of standard
infection control precautionsProvide a barrier to spread of
infection
Part of personal protectiveequipment (PPE) against contact
with blood during phlebotomy
Good fit is essential
Washing or reuse of gloves can compromise integrity of material to serve as barrierwithout visible changes.
Gauze Pads & Bandages
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Gauze Pads & Bandages
Gauze Pads
Should be clean
Used to apply pressure on site after
needle removal
Adhesive Bandages/Tape
Used to secure gauze
Do not apply directly on the site
Cotton not recommended as fibers can stick to site and initiate bleeding
when removed Do not use alcohol swab
Antiseptic and Disinfectant
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Antiseptic and DisinfectantAntiseptic
Inhibit or prevent the growth of bacteria
Approved for used on the skin
Used to clean venipuncture site
70% isopropyl alcoholmost commonly used
Disinfectant
Kills bacteria and inhibits some viruses
Check manufactures label
For use on surfaces and instruments, not on skin
Used to clean up all blood spills
1:10 hypochlorite (bleach) solution commonly used
Isopropyl alcohol
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70% optimal concentration asantiseptic
Store in closed container
Active ingredient evaporates fromopen container leaving water behind
Antiseptic properties diminished Bacteria from hands, container can
multiply and cause infection inpatient if used to clean intendedpuncture site
Cleaned site may not dry quickly
Isopropyl alcohol
Never pre-soak cotton balls
Cleaning the Site
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Cleaning the Site
Use pre-packaged swab or cotton ballthat has been moistened with antiseptic
at time of use on patient
Clean area in a spiral starting at theintended site of puncture moving
outward
Let air dry
Do not blow or fan site
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Clinical And Laboratory Standards Institute
Syringe and Needle Blood Collection
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Syringe
Variety of sizes:
2ml, 5ml,10ml, 20 ml Selection depends on
Patient
Volume of blood to collect
Strength of vacuum created
Advantage
Blood flash can be seen on
entering vein
Disadvantage
Specimen can clot
Specimen must be transferred
Never exert pressure on plunger in
transfer to vacuum tube
Vacuum to draw blood
from vein throughneedle and into syringe
created as plunger
withdrawn
User controls thevacuum
Syringe and Needle Blood Collection
Venipuncture Procedure Using Needle
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p gand Syringe
After needle is in the vein,slowly pull the plunger back
to start filling the syringe.
While blood flows into the
syringe, release the
tourniquet.
Transfer of Blood From Syringe into
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Remove the cap of the container
and gently transfer specimen
into it by pushing the plunger
Ensure there is no froth
formation during the blood flowinto the container
Do not overfill
Replace the container cap
y gSpecimen Container
Transfer of Blood from a Syringe into a
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Do not remove rubber stopper when using
evacuated tubes. Place the tube upright in a rack.
Slowly pierce the stopper of the evacuated tube
with the needle.
Allow the tube to fill (without applying pressure
to the plunger) until blood flow into the tube
ceases. This technique helps to maintain the
correct ratio of blood to additive.
Follow the same order for filling tubes as the
order of draw for an evacuated system.
y g
Evacuated Tube
1. WHO guidelines for drawing blood: best practices in phlebotomy, November 2009
Evacuated Closed Blood Collection
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Evacuated Closed Blood Collection
Vacuum in tube allowsblood to be drawn directly
from vein into evacuated
tube
No need to transfer blood
Tube Filling evacuated system
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g y Maintain downward position so blood and additive do not
touch non-patient end of multi-sample needle.
Allow to fill until vacuum is exhausted and flow stops. Remove from holder by applying pressure against wings*
of holder with thumb and index finger. This assists inholding needle steady as tubes are removed and inserted.
Invert gently several times after removal to mix blood andadditive. Additional mixing per tube type can be performedwhile next tubes are filling.
Continue to draw tubes following correct order of draw.
* As for tube placement above, this is critical to thesuccess of the procedure.
Order of Draw
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Order of Draw
(with clot activator)
1. Sterile samples (eg: Blood Cultures)
2. Citrate tubes*
3. Plain Serum tubes
4. Heparin tubes
5. EDTA tubes
6. Fluoride Oxalate (glucose tubes)
7. ACD tubes
* this tube is acceptable for routine coagulation testing (e.g. aPTT and PT/INR). For some special coagulation testing where low level activation of coagulation
factors may be of particular concern**, the use of a non-additive discard tube may be considered1. Because of dead space in the tubing of winged (butterfly)
sets, a discard tube should also be used where citrate tubes are the first drawn and a winged collection set is used. This is particulally important when 1.8mL and
2.7mL tubes are used.
** activation may be induced by tissue factor (tissue thromboplastin) introduced into the initial sample stream as the needle traverses the subcutaneous tissue.
Note regarding ESR: in accordance with CLSI1 recommendations, ESR tubes should be placed at position 2 above.
1. CLSI document H3-A6 Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture. 6th Edition, 2007
Mixing of Tubes
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Why
All tubes contain
additive that needs to be
mixed with the blood
sample.
Tubes withanticoagulants such as
EDTA need to be mixed
to ensure the specimen
does not clot.
How
Holding tube
upright, gently
invert 180 andback.
When Immediately after
drawing.
Consequences if not mixed:
Tubes with anticoagulants will clot
Specimen will often need to be redrawn
Mixing of Tubes
Needle Disposal
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Needle Disposal Ifa single-use holder is used, the complete assembly should
bediscarded into a puncture-proof sharps container.
Otherwise - The contaminated needles must be safely removed from the
holder and discarded in a suitable sharps container1,2
Nevercut, bend, break, burn, or re-cap needles.
A variety of containers is available. Most have a facility3to
provide safe removal of the needle from the (re-usable) holderwhen using an evacuated tube system.
1. Needles must not be removed with fingers. If needle doesnot separate from the holder, the entire assembly may bediscarded in the sharps container.
2. Place needle in the proper slot in the lid, and rotate anti-
clockwise until it unscrews from the holder. Allow theneedle to drop into the container
Sharpcontainers should not be overfilled
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Post venipuncture
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p
The patients arm is examined to see if bleeding hasstopped.
An adhesive bandage or tape is applied on the site(following institutional policies).
The patient is instructed to leave the bandage on for aminimum of 15 minutes.
Out patients should be advised not to carry a purse or otherheavy object or lift heavy objects with that arm for 1 hour.
The patient should be thanked for his or her cooperationthis helps leave the patient with a positive feeling.*
(Contaminated materials should be disposed of in approvedbiohazard containers following the institutional policiesbefore attending to the next patient.
*Reference: Ruth E McCall & Cathee M. Tankersley. "Phlebotomy Essentials" 3rd edition (2003) p 272.
Tube Transportation
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p Tubes transported through public hallways should be placed in a
secondary container to minimize the risk of leakage and spillage.
The secondary container shouldbe clearly labeled "BIOHAZARD.
Once the tubes are in the container, they should be sealed prior totransport.
Once primary containers are inside the externally uncontaminatedsecondary container, they can be handled without gloves2.
Personnel who transport specimens should be trained in safe handlingpractices and decontamination procedures2.
Paper requisition(s) and other documents (if present) mustbe attachedsecurely to the secondary transport container.
All necessary steps shouldbe taken to avoid exposure of tubes tomechanical trauma, temperature extremes and delays duringtransportation as these can introduce significant pre-analytical error1.
Once received in the laboratory, gloves should be worn while removingspecimens from the secondary container and for all manipulations ofprimary container2.
1. CLSI Document H18-A3. Procedures for the Handling and Processing of Blood Specimens; Approved Guideline, 3rdEdition, 2004
2. CLSI Document M29-A3 Protection of Laboratory workers from occupationally acquired infections; Approved Guidelines,3rd Edition, 2005
Special Collection Procedures
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p Use of winged collection set is recommended for all blood
culture collection procedures.
Thorough skin preparation is essential* The site should first be cleansed with 70% alcohol
The site should then be swabbed with 1-10% povidine-iodine solution or chlorhexidine gluconate (the latter isrecommended for infants greater than 2 months of ageand patients with iodine sensitivity) by circular motion,
starting in the middle. The site must then be allowed to dry. The iodine /
chlorhexidine can then be removed with an alcoholswab.
* institutional policies and procedures must befollowed
The culture bottle stopper should be disinfected followingmanufacturers instructions.
Summary
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y
Prepare the tray. Ensure all the logistic/equipment areavailable in the tray
Choose the correct site for cannulation.Avoidjoint/obliterated vein
Follow aseptic technique, wash hand,clean cannulationsite and wear clean gloves
Maintain record of cannulation
Watch for complication like phlebitis/haematoma etc.
Replace cannula every 72-96 hours
Dispose sharps and other materials appropriately as per
guideline(CPCP/SPCB)