Modulation of the human gut microbiota by short chain ... · Modulation of the human gut microbiota...
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Modulation of the human gut microbiota by short chain Fructo-oligosaccharides
Ashok Kumar Dubey, Ph.D. Tata Chemicals Ltd Innovation Centre
ILSI Conference, 6th December 2018
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Humans: More Microbes Than Human Cells?
100 trillion bacterial cells 100X more bacterial cells than host cells 1000X more bacterial genes than host genes
MICROBIAL ROOTS
SUPRA-ORGANISMS: CONGLOMERATES OF MAMMALIAN AND MICROBIAL CELLS
Archaea Bacteria Fungi Protozoans
THE INTESTINE (~100 trillion organisms)
SITE OF LARGEST COLLECTION
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Prebiotics: Beneficial Modulation of the Gut Microbiome
Gut Microbes Prebiotics Consume Producing
Metabolites (SCFAs)
Nutrient absorption and metabolism
Immune response
Cell-to-Cell signaling
Can be applied in
Metabolic Syndromes (Obesity, Type II Diabetes, Cardiovascular Diseases)
Cognitive Health (motor activity and memory retention)
Chronic Disorders (ulcerative colitis, IBS, and Crohn’s Disease)
Creation of acidic environment (decreased
pH) Increasing good bacteria
Reduce pathogenic microbial abundance
through
This influences
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TATA-Yale Collaborative Study: Design
Germ free Mice
Colonized with human gut microbiota
Fed customized diet with FOS
Fed customized diet without FOS
FOS Group
Control Group
-7d Gut microbiota
(Gavage)
0 d
7 d
14 d
21 d
28 d
Fecal Sample Collection
Mice euthanized
Intestinal content
collected
DNA sequencing on Illumina
Miseq
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FOS Supplementation response
Snapshot of microbial relative abundance
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FOS promoted the growth of Bifidobacterium , Faecalibacterium prausnitzii,
Blautia and bacteria belonging to Lachnospiraceae family
Bio-geographical and Spatial arrangement of gut bacteria play a role in
determining the impact of prebiotics
Utilization of FOS by good gut bacteria results in reduction of Sutterella, a
pathogenic bacteria
Consumption of FOS enhanced production of SCFAs
Summary
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Impact of Fructo-oligosaccharides (FOS) on human gut microbiota: A clinical trial
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71.5
18.7
3.8 0.6 5.4 Relative Abundance (%)
Bacteroidetes
Firmicutes
Proteobacteria
Actinobacteria
Others
95% of sequences were found to belong to microbes belonging to the four phyla
Remaining 5% belonged to 16 other phyla
More than 80% of sequences were assigned to 5 genera - Prevotella, Faecalibacterium, Alloprevotella, Roseburia, and Bacteroides
Two distinct clusters were obtained Cluster 1 was further divided into 2
subclusters subcluster 1a (core taxa): Bacteroides,
Faecalibacterium, and Roseburia Subcluster 1b (core taxa): Bacteroides,
Faecalibacterium, Bifidobacterium, Blautia, Dorea, Lachnospiraceaincertaesedis, and Streptococcus
Cluster 2 (core taxa): Prevotella and Faecalibacterium
Analysis of basal phase sample revealed uniqueness
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Prevotella, Faecalibacterium were predominantly present in the Western
Indian subjects
FOS consumption increased the relative abundance of OTUs belonging to
Bifidobacterium and Lactobacillus.
FOS at higher dosage appear to promote the selective proliferation of OTUs
belonging to Lactobacillus.
A significant change was also observed in certain butyrate-producing
microbes
Positive impact of FOS on butyrate-producing bacteria reinforces the role of
prebiotics in conferring beneficial functions to the host.
Summary
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Acknowledgement Noah Palm Deguang Song David Urbonas Gary Cline Sharmila Mande Anirban Bhaduri
Funding by Tata Chemicals as a part of TATA-YALE Collaboration
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Thank you