mmi® CellCut Plus® Laser Microdissection...
Transcript of mmi® CellCut Plus® Laser Microdissection...
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TechnologyTransition Workshop
mmi® CellCut Plus®Laser Microdissection System
Technology Transition Workshop| Lee Petruk
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TechnologyTransition Workshop
mmi® CellCut Plus®
mmi® SmartCut plus®
mmi® CellEctor®
mmi® CellManipulator®
MMI® Service & Support
MMI® Application Support
MMI® Product Range
Physical Separation of Forensic Mixtures Using Laser Microdissection Techniques2
mmi® CellCut Plus®
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mmi CellCut® System Components
• Olympus® IX71 or IX81
• Nikon® Eclipse Ti inverted microscope
• Standard objectives - 4X, 10X, 20X and 40X
• High precision XY stage
• Solid state UV laser
• Cooled CCD color digital camera
• Intuitive mmi® software
• Computer and monitor
• Pen touch screen
• Various options
Physical Separation of Forensic Mixtures Using Laser Microdissection Techniques3
mmi® CellCut Plus®
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Characteristics of the Laser
• Compact design
• Solid state diode-pumped YAG Laser, 355 nm
• Long life span – approximately 10,000 hours
• Quasi continous performance pulse time 0.6 ns
• Laser cutting sub-micron at 40X and higher
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mmi® CellCut Plus®
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Advantage of MMI® Laser
• MMI® uses less laser power at a higher frequency to minimize collateral damage
Physical Separation of Forensic Mixtures Using Laser Microdissection Techniques5
mmi® CellCut Plus®
®
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Cutting Examples of MMI® Laser
Physical Separation of Forensic Mixtures Using Laser Microdissection Techniques6
mmi® CellCut Plus®
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Materials Used for LCM
• Paraffin samples
• Cryo samples
• Archival slides
• Smears
• Cytospins
• Live cells
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mmi® CellCut Plus®
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Specimen
Membrane
- frame
Glass slide
Result:absolutely
“contamination-free” samples!
Slide Preparation
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mmi® CellCut Plus®
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Contamination-free Microdissection • mmi® MembraneSlide® and a regular glass slide
protect your sample from environmental contamination
• Sterilization/cleaning of the membrane before microdissection possible
• mmi® MembraneSlide® can be handled in the same way as a glass slide
• Superb image quality
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mmi® CellCut Plus®
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Automatic mmi® CapLift®
• Software-controlled
− Positions the cap above the cutting area
− Places the cap onto the membrane
− Lifts the cap
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mmi® CellCut Plus®
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mmi® IsolationCaps® Multicap Option
• Available in 0.2 µl eight cap strips
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mmi® CellCut Plus®
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mmi® IsolationCaps® – Three Collection Cap Sizes
• 0.2 µl
• 0.5 µl
• 1.5 µl
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mmi® CellCut Plus®
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User-friendly Software
• Single page
• Icon driven
• Multi-group drawing and cutting
• Whole slide scanning
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mmi® CellCut Plus®
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mmi® CellTools® Software
• Icon driven
• mmi® IsolationCap® automatically collects the samples over full slide
• Whole slide scan for easy navigation
• Automatic documentation
• Multi-user software
• Software modular with other MMI® instruments
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mmi® CellCut Plus®
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• Example for advanced mmi® CapLift® settings:
− Video panel
− Cap surface:
• PTP* algorithm
mmi® CapLift® Settings
* Predefined target positioning
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4 5
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mmi® CellCut Plus®
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mmi® CellTools® – Group Cuttings• Definition and editing
of groups
• UVCut → Draw and Cut → Group Editor
• Automated cutting of one, several, or all groups
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mmi® CellCut Plus®
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Pen Touch Screen Option
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mmi® CellCut Plus®
To see a video of the pen touch screen in action, go to: http://www.molecular-machines.com/download_all.html - from the list of video downloads select Cell Isolation – Pen Screen
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mmi® CellTools® – Documentation
• Pictures before and after microdissection
− Save as TIF, JPEG, BMP or TGA
− Number of cuts recorded
− Tissue area in square microns
− User name and file settings
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mmi® CellCut Plus®
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Isolation of Sperm
Anti-sperm ABAcridine orange
Gills
HE
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mmi® CellCut Plus®
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Old CaseTransferred cells on
membranestained with mmi® H&E
Staining Kit for LCM
Glass slide after transfer
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mmi® CellCut Plus®
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mmi® CellExplorer®
• Software solution developed for automatic cell identification with the mmi® CellCut®
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mmi® CellCut Plus®
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mmi® CellExplorer®
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mmi® CellCut Plus®
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mmi® SmartCut®
• Olympus® CKX41 microscope or Nikon® T100 base with 4x and 20x objectives
− Other objectives optional
• Computer controlled XY stage
• Solid state laser, 355 nm wavelength
− Same specs as with mmi® CellCut®
• Digital camera with computer and 17-inch monitor
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mmi® CellCut Plus®
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The mmi® CellEctor®
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mmi® CellCut Plus®
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mmi® CellEctor®
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mmi® CellCut Plus®
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Summary• Best laser in industry for microdissection, first in diode
UV laser technology
• Contamination-free sample due to membrane slide and collecting cap technology
• Easiest handling of the system due to intuitive icon driven software
• Solution for manual and automated systems
• Modular system can be expanded and can be combined with mmi® CellEctor®
• Your choice of Nikon® Ti or Olympus® IX series microscope
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mmi® CellCut Plus®
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Thank you for your attention.
Questions?
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mmi® CellCut Plus®
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Contact Information
Lee Petruk
Molecular Machines & Industries
www.molecular-machines.com
All images copyright Molecular Machines and Industries®
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mmi® CellCut Plus®