8/7/2019 Millicell uMigration Assay Kit

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A powerful, slide-based platform

that measures the effects of 

chemoattractants on the migration

of adherent single cells through

real-time imaging.

To see how the Millicellµ-migration slide works, visit:www.millipore.com/umigration

Millicell® µ-Migration Assay Kit

Benefts

• Establishes a stable and linear concentration

gradient after 1 hour that lasts ≥48 hours

• Distinguish chemotaxis from random

movement

• Multiparametric analysis enables greater

mechanistic insight

• Enhanced optical imaging for slow- and fast-

migrating cells• Analyze three chemoattractants in parallel for

increased throughput and analytical power

• Ready to use, no assembly needed

A typical example of cells with tracked migration paths. Enhanced

optical imaging makes the µ-migration slide especially suitable for

high-quality and fluorescence microscopy, perfect for observing

single cells.

8/7/2019 Millicell uMigration Assay Kit

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70 µm1 mm

8 hrs

24 hrs

48 hrs

Millipore, Millicell, EmbryoMax, and Advancing Life Science Together are registered trademarks

and the “M” mark, Scepter, and Accutase are trademarks of Millipore Corporation.

Lit. No. PR3196EN00 Rev. B 02/11 LS-SBU-10-03833 Printed in U.S.A.

© 2011 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.

Use the Millicell µ-migration slide to measure parameters

 you’ve never been able to quantify before, such as:

• Migration velocity

• Migration paths of individual cells

• Directionality

• Migration index of tracked cells

You won’t get this kind of information with traditional

migration assay platforms such as Boyden chambers or

scratch (wound healing) assays.*

HT-1080, HUVEC, MDA-MB-231, and NIH-3T3 cell lines

have been shown to work effectively with the Millicell

µ-migration kit.

Center of mass [µm]: x = 0.97 y = 6.41

Number of tracks: 80Counts up: 51Counts down: 29

x axis [µm]

  y  a  x   i  s   [  µ  m   ]

300

200

100

0

-100

-200

-300-300 -200 -100 0 100 200 300

Center of mass [µm]: x = 0.97 y = 37.25

Number of tracks: 78Counts up: 44Counts down: 34

x axis [µm]

  y  a  x   i  s   [  µ  m   ]

150

100

50

0

-50

-100

-150-150 -100 -50 0 50 100 150

ell Tracks% FCS 10% FCS

Side view of a chamber.

0% FCS 10% FCS

Positivex-axismigration

Positivey-axismigration

   M   i  g  r  a   t   i  o  n  v  e

   l  o  c   i   t  y   (  µ  m   /  m   i  n   )

0.14

0.12

0.1

0.08

0.06

0.04

0.02

0

-0.02

The Millicell µ-migration slide establishes a stable linear

concentration gradient across the observation window th

lasts 48 hours.**

**The calculated maximum concentration is only 70% of the applied

concentration. The final maximum concentration is only 33% of the

applied concentration. Compare this with a Boyden chamber where

very steep gradients may form along a single axis perpendicular to th

surface of the membrane.

Image examples o luorescence measurements

Relative intensity across a 1 mm slit (raw and smoothed data

www.millipore.com

-0.05

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

0.2 0.4 0.6 0.8 1

 

t = 2hrs

t = 4hrs

t = 8hrs

t = 24hr

t = 48hr

Linear (t

Linear (t

Linear (t

Linear (t

Linear (t

  n  o  r  m  a   l   i  z  e   d   i  n   t  e  n  s   i   t  y

distance (mm)

HT-1080 cells migrate aster in presence o 10% FCSchemoattractant

*Data was analyzed and graphed using a free Image J software plug-in.RRI IFRMATI

Description Qty/Pk Catalogue No.

NEW Millicell µ-Migration Assay Kit 1 kit (4 slides,

12 assays)

MMA205

Required Equipment/Reagents

Description Qty/Pk Catalogue No.

Accutase™ Cell Dissociation

Solution

100 mL SCR005

Dulbecco’s Phosphate Buffered

Saline, 1X ES Cell Qualified

500 mL BSS-1005-B

EmbryoMax® ES Cell Qualified Ultra

Pure Water, sterile H2O

500 mL TMS-006-B

EmbryoMax ES Cell Qualified Ultra

Pure Water, sterile H2O

100 mL TMS-006-C

Scepter™ Handheld Automated

Cell Counter

1 ea PHCC00000