Microscopy and Photomicrography

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During Dr. Robert Ross’s workshop, we worked with different microscopies and micro techniques. Some examples of the microscopies are Bright-field, Phase Contrast, and Dark-Field. Dr. Ross explained the function and use of Scanning Electron Microscopy and the Transmission Electron Microscopy. He also explained micro-techniques such as whole mount, cross section,longitudinal-section, and squash. As part of this workshop, we used the Dissecting Scope, Phase Contrast Microscope and the Bright Field Microscope. Dr. Ross taught us a little about the history of the different microscopes. Later, each one of us instructed our partner in the usage of a particular microscope. In my case, I learned how to use the Phase Contrast Microscope and then I taught Felix how to use it. By using the Phase Contrast, you can discover structures that are not visible with a Bright-field microscope. Fernando taught me how to use the Dissecting Scope. I looked for the fern Polypodium, and then I used a blade to cut a small piece of the spores and a piece of the entire leaf with all the sporangia in order to observe it under the microscope. Next, I compared my pictures with Internet images. As part of the results, I did not find in Internet a Phase Contrast image of Polypodium. Instead, I looked for an analogous picture with a similar magnification. The usage of microscopes is very important, because with it we can observe and investigate many things such as microorganisms and parts of cells. Microscopes also help in the discovery of diseases and in how they can be treated, because diseases are too small to be seen by the human eye. Microscopes provide us the ability to see at a cellular and a molecular level.

Transcript of Microscopy and Photomicrography

Page 1: Microscopy and Photomicrography

During Dr. Robert Ross’s workshop, we worked with different microscopies and micro techniques. Some examples of the microscopies are Bright-field, Phase Contrast, and Dark-Field. Dr. Ross explained the function and use of Scanning Electron Microscopy and the Transmission Electron Microscopy. He also explained micro-techniques such as whole mount, cross section,longitudinal-section, and squash. As part of this workshop, we used the Dissecting Scope, Phase Contrast Microscope and the Bright Field Microscope. Dr. Ross taught us a little about the history of the different microscopes. Later, each one of us instructed our partner in the usage of a particular microscope. In my case, I learned how to use the Phase Contrast Microscope and then I taught Felix how to use it. By using the Phase Contrast, you can discover structures that are not visible with a Bright-field microscope. Fernando taught me how to use the Dissecting Scope. I looked for the fern Polypodium, and then I used a blade to cut a small piece of the spores and a piece of the entire leaf with all the sporangia in order to observe it under the microscope. Next, I compared my pictures with Internet images. As part of the results, I did not find in Internet a Phase Contrast image of Polypodium. Instead, I looked for an analogous picture with a similar magnification. The usage of microscopes is very important, because with it we can observe and investigate many things such as microorganisms and parts of cells. Microscopes also help in the discovery of diseases and in how they can be treated, because diseases are too small to be seen by the human eye. Microscopes provide us the ability to see at a cellular and a molecular level.