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![Page 1: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/1.jpg)
Microscopy and Cytology
p 73
![Page 2: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/2.jpg)
Compound Light Microscope
• Review proper use and care of microscope by watching video.
• Principles of microscopy p 78
![Page 3: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/3.jpg)
A.Magnification
• Factor by which specimen is enlarged
![Page 4: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/4.jpg)
B. Parfocal and parcentric
• Image will remain in focus and centered as you switch from one objective to the next.
![Page 5: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/5.jpg)
C. Size of microscopic field
• Using a ruler, you will measure the diameter of the field using 4X scanning objective.
• Your measurement will be somewhere near 4.2 mm
• Ruler is in mm. Convert to micrometers
• 4200 micrometers (um)
![Page 6: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/6.jpg)
• Formula to determine unknown field of view if field of view of scanning objective is known.
![Page 7: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/7.jpg)
40 X X 4200 um
Total magnification of unknown field
Field of view of scanning objective
Total mag with scanning objective
![Page 8: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/8.jpg)
40 X 4200 um
100Total magnification with low power objective
Field of view of scanning objective
Total mag with scanning objective
Answer: 1680 um
![Page 9: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/9.jpg)
Calculating the field of view
Objective mag
Ocular mag
Total mag Diameter (mm)
Diameter (um)
Scan 4X 10X 40X 4.2 4200
Low 10X 10X 100X N/A 1680
High 40X 10X 400X N/A 420
Oil 100X 10X 1000X N/A 168
![Page 10: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/10.jpg)
• If the size of bacterial cells is in the range of 1-10 micrometers, which objective should be used for viewing bacterial cells?
![Page 11: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/11.jpg)
D. Depth of focus
Remember to focus on each individual thread to determine which is on top.
Defined as the vertical distance that an object remains in focus at one time.
![Page 12: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/12.jpg)
E. Image orientation
• View the letter E slide
If you place the slide face up and right side up on the stage, how does the “e” appear when viewed through the microscope?
![Page 13: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/13.jpg)
F. Resolving Power
• Degree at which 2 points on a specimen are seen as separate images
![Page 14: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/14.jpg)
G. Contrast
• The degree to which details of a specimen stand out against the background
![Page 15: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/15.jpg)
• Omit section “V. Other types of microscopes”
![Page 16: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/16.jpg)
Microscopy
• Answer questions on microscopy on p 84-86.
• Label parts of microscope.
• You will need to know these concepts for the lab practical.
![Page 17: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/17.jpg)
Part II:The Cell p 89
• Review information on cellular structures and organelles. This material will be a review of what you have covered in lecture.
• For the lab practical, I will only ask questions about the structures that we view in the lab.
![Page 18: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/18.jpg)
Exercise 7.5 p 95
• Look over drawing and models of plant and animal cells.
• Concentrate on identifying these structures:– Nucleus, nucleolus, plasma membrane,
cytoplasm, chloroplast (in plant cells), cell wall (in plants), vacuole, cilia, flagella
![Page 19: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/19.jpg)
Exercise 7.6 p 96
• A. Cyanobacteria-omit• B. Elodea leaf- prepare a wet mount, view and
sketch (use a drop of water, then add safranin)• C. Onion leaf- prepare wet mount using iodine.
Use a very thin section. Draw and label.• D. Stained cheek cell- prepare wet mount
stained with methylene blue. Draw and label.• E. Ear swab- omit
![Page 20: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/20.jpg)
Cheek cells at 10X
![Page 21: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/21.jpg)
Cheek cell at 1000X (using 100 X oil objective)
![Page 23: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/23.jpg)
All photos by Jeff Beck, CCCCD unless otherwise noted.
![Page 24: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/24.jpg)
http://commons.wikimedia.org/wiki/Image:Microphoto-cells-onion2.jpg
Onion cell
![Page 25: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/25.jpg)
Exercise 7.7 Bacterial slides p.102
• You must use OIL IMMERSION objective to view bacteria.
• If you are not sure how to use oil, ask!• Find the area to view using scanning, 10X and 40X
objectives, then add oil and move to 100X objective.
• Always find the area with scanning objective first, then move to higher magnifications
• Detailed instructions for using the oil immersion objective http://biology.clc.uc.edu/fankhauser/Labs/Microscope/Oil_Immersion.htm
![Page 26: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/26.jpg)
• View prepared slides of bacterial cells.
• Gram-stain– Purple- gram positive– Pink to red- gram negative
• Cell shapes– Coccus– Bacillus– Spirillum
![Page 27: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/27.jpg)
Rods- bacillus
![Page 28: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/28.jpg)
Spherical-coccus
![Page 29: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/29.jpg)
Corkscrew- spirillum
![Page 30: Microscopy and Cytology p 73. Compound Light Microscope Review proper use and care of microscope by watching video. Principles of microscopy p 78.](https://reader035.fdocuments.us/reader035/viewer/2022081514/56649ecf5503460f94bdd666/html5/thumbnails/30.jpg)
p103
• Study drawing of bacterial cell
• Answer lab questions #2 and #3 on p 104-105.