Microscope Introduction

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Introduction to the Microscope Care Parts Focusing

Transcript of Microscope Introduction

Page 1: Microscope Introduction

Introduction to the Microscope

CarePartsFocusing

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• Magnification – making the object bigger

• Resolution – how clearly you can see the object

• http://brunelleschi.imss.fi.it/esplora/microscopio/dswmedia/simula/esimula1.html

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“Light” because we use light to see the images

“Compound” because it uses two lenses to view a specimen.

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Light vs. Electron

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Light vs. Electron

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Light vs. Electron

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SEM vs. TEM

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Eyepiece orOcular Lens

Body Tube

Revolving NosepieceArm

Objective Lenses

StageStage Clips

Coarse Focus

Fine Focus

Base

Diaphragm

Light

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• Always carry with 2 hands - by the arm and the base.

• Only use lens paper for cleaning and don’t touch the lenses to anything (including your fingers.)

• Always store covered• Don’t let cords hang where the

microscope could be pulled off the table.

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• Ocular lens X Objective lens

• If the ocular lens is 10X and the objective lens is 5X, the total magnification would be:

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• Place the Slide on the Microscope• Use stage clips only when you are sure

you don’t want to move the slide around.

• Click Nosepiece to the lowest (shortest) setting

• Start with the objective closest to the specimen.

• Use the Coarse Focus to move objective AWAY from specimen.

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• Have focused on low power.

• Click the nosepiece to the next biggest objective.

• Do NOT use the Coarse Focusing Knob

Use the fine focus knob to bring the slide

• Do NOT force any knobs. They should all move easily.

• Do NOT turn the fine focus more than a full turn. Start over on low power if you have to.

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Elodea

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Onion Cells

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Cheek Cells


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Microscope introduction

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