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Introducing… The Ultimate Electrophysiology Workstation

Hiroshi NishidaNikon Instech Co. Ltd

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MethodDesigned with Electrophysiologists for Electrophysiology Research

1. The Development of the FN1 began by gathering specifications consulting with top scientists in Asia, Europe and the US market.

2. In the next phase, meetings were held to review the information gathered, and set the optical and mechanical specifications for prototype development

3. Prototypes were built, including optics, stages and translation, and they were evaluated by researchers in Asia, Europe, and the US.

4. These evaluations confirmed that the specifications were what the market wants and needs.

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University ResearchersStony Brook University, Stony Brook, NY. Dr. Lonnie P. Wollmuth

Cornell University, Ithaca, NY Dr. Joseph Fetcho

Cold Spring Harbor Labs, Cold Spring Harbor, NY Dr. Karel Svoboda, Dr Yasuda Ryohei

Memorial Sloan-Kettering Cancer Center, NY, NY Dr. Ricardo Toledo Crow

NYU Medical School, NY, NY Dr. Gan

Harvard Medical School, Boston, MA Dr. Bernardo Sabatini

Brown University, Providence, RI Dr. Mark Bear, Dr. Barry Connors, Dr. Anna Dunaevsky

Yale University Medical School, New Haven, CT Dr. Dejan Zecevic, Dr. John P Geibel

University of Pennsylvania, Philadelphia, PA Dr. Philip Haydon

University of California, Irvine, CA Dr. Ivan Soltesz

LSU, New Orleans, LA Dr. Jeff Magee

Kaiserslautern University, Germany Dr. E Friauf

Homberg University, Germany Dr. Peter Lipp

University College of London, London England Dr. Michael Hausser

Max Planck Institute, Heidelberg, Germany Dr. Bert Sakmann, Dr. Feltzmeyer

Tokyo University, Japan Dr. Tsujimoto, Dr Kudo, Dr. Miyakawa

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Main Market:FN1■Electrophysiology Market

Sample: Brain slice of small animal measuring weak electric signals of the brain neuron

■ Physiology market　 Sample: organ of a small animal (alive sample)Visualizing blood flow of the animal. Measuring the electrolyte concentration difference of the cell (Inside/outside) – Combination of medication or electric stimulation

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What is Patch Clamp?

• A method to fix cells with glass pipettes and measuring the electric current and potential of a cell, cell membrane or a group of cells

• Information are transferred to the cell by electric current and change of potential, therefore measured to know the activities and functions of a cell or cells

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×

Brain slice

Thickness : 300-500um

Petri Dish

Objective lens

electrode pipette Micromanipulator

Example of use : Electrophysiology

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Setting the sample

Making electrode

Sample preparation

Setting electrode pipette

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Mounting electrode pipette

electric stimulation

Around the stage

Noise reduction

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■Easy electrode mounting (Microscope shape)

■Anti-vibration during electrode operation or observation

■Anti-noise (During electrolyte measurement) 　　■Easy visualization and high resolution recording of the

minute structure of the cell 　　

■Ergonomics (operationality)

■Easy modification

Requirements from the market

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Open Space

■ ”I” shaped body– Minimum projection from microscope bod

y to give maximum space around the sample.

– Open space for manipulators, stimulators, flow chambers, etc. for electrophysiology

– Low eyepoint

■ Small filter cube turret– By using filter cube located nearside of th

e observer, turret body would not stick out. (*J-EPI)

Inherit good repute of E600FN, but also improved it.

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Open Space■ Open space under the condenser

– Having open space under the condenser would give users put extra filters or prisms.

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Easy sample setting■Open Space

– Main body pillar pushed back to allow close to 360 degrees access around the objective lens

■ Water dipping mechanism for both objectives– 15mm Up Down movement for both obj.

lens– No need to move focusing knob to pick u

p liquid surface for water dipping lens.– Wide variety of objective lens selection

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Easy sample setting■ Centering and parfocal adjustment assembly for water

dipping objective lens– Easy operations for centering and focusing to adjust the view

of both back and forward objectives, observer will not loose their needle tip.

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ErgonomicsFront (concentrated) operation assembly■ Most operations can be done on the lower

front part of the microscope– Coarse and fine focusing handle– Refocusing mechanism– Filters for IR-DIC– Shutters, and illumination for bright field and fluores

cence light sources.■ New LWD condenser

– Easy remove– Brightfield, DIC, Oblique Illumination is possible un

der one condenser. – The new water proof element condenser lens– Oblique diaphragm can be rotated 360 degrees to e

nhance specimen details and orientation

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Ergonomics

■ Raising objective height with extra arm enables larger specimen handling.

■ Specimen space can be increased over 40mm. Vivo imaging and Biochip experiments requiring larger and thicker specimen space is possible.

Mechanism to observe alive specimen

NikonPlan 100X 1.1NA Dipping LensTrancedental image with the new water dipping objective

In-vivo Multi-Photon imagingof dendritic spines in the cortexa live whole mouse(Michael Hausser, Ph.D. University College of London)

•The first dipping lens made with a correction collar for depth induced spherical aberration correction, providing exceptional IR-DIC resolution and superb confocal performance

•2.5mm of Working Distance

•High IR Transmission

Aberration adjustment works fine up to Two-photon excitation wavelength

Surface 100nm Aberration corrected

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New water dipping objective lens• 17% Slimmer objective lens. easy access f

or micromanipulator– Access angle

– 40x 45 degrees– 60x 27 degrees– 100x 24.4 degrees

• aberration corrected upto near infrared– High contrast IR imaging

New Slim CFI60 PL FL 10XW, 0.3NA, 3.5mm WDNew Slim CFI60 Apo 40XW NIR, 0.8NA, 3.5mm WDNew slim CFI60 Apo 60XW NIR, 1.0NA, 3.0mm WDCFI75 16X 0.8NA, 2.5mm WD

with a usable 45 Approach angleCFI60, Plan 100XW, 1.1NA, 2.5mWD

with temp calibrated correction collar. ultimate lens for Multi-Photon applicatio

ns

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Dedicated New objectives lens for electrophysiology

16x, NA0.8, WD3.0 Objective lens Large access angle – 38.2 degreeLong working distance – 3.0mm 　 Working Distance longest among the class ）

For easier operation and manipulator handling, WD 3.0mm WD new water dipping objectives is now available.

New Multi Magnification Tube lens unitWith the combination of 16x objective and Multi Magnification Tube lens unit (0.25x), wide field of view observation is possible. (0.35x,2x,4x)

2.0mm （ widest among the class ）

2.0mm view gives easy micro needle handlingLow mag. is effective for whole slice imaging

45° 45°3mm

45 approach angle!!

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0.35x for quick needle setting2x for easy cell searching4x for comfortable patch-clamp

The True Single Objective Solution: LWD 16X

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IR & Visible DIC System

IR and Visible Analyzer Slider

NCB Filter and ND turret w/ ND2, ND4, ND16 & open position

IR (850-950) and Visible Polarizer Turret (with alignment tool)

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Two IR options: IR-DIC and IR Oblique850-950nm IR for deeper specimen penetration

Two Visible options: DIC & ObliqueSatisfies all customer needs

Visible & IR DIC and Oblique Illumination

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You can select the contrast orientation without rotating your sample

IR-DIC (850-950nm) Oblique ILL. (850-950nm)

CFI 40x (NA0.8)

IR-DIC and Oblique Illumination

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Depth 0 um Depth 0 um

Depth 120 um IR-DIC (850 to 950nm)

Depth 120 um (Vis to 750nm)

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Avoiding Vibration noise■ Anti click vibration architecture

Improved Stand Rigidity (CAD)

Mechanism of avoiding vibration conduction is applied for cube turret, optical path changer, magnification changer, condenser filter changer.

Solid Objective changerBody tubes T & F with detent release lever.Mag changer with soft detentRear mag changer on dual port with soft deten

tCondenser Turret with soft detent

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Avoiding electrical noiseElectrical noise free■ Groundings　Special attention was made to

reduce electrical noise to an absolute minimum.Ground pin can be connected to the main components of the microscope to decrease electrical noise.

■ Fiber opticsAs a standard, Fiber optics has been employed as the light source. Power unit can be placed out of the cage to avoid electric noise.

Previous FN1

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Three Plate Stage

• Easy Access from front

• Support pillars on side

• Includes 20mm insert & special chamber

• Accepts all standard Nikon condensersBy removing the stop screw

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“Magnetic scale”

d

• Mounts in rear of stand• Measure depth of sample to 1µ• Refocusing when changing electrodes

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Two Illumination Systems• New 12V/100W Fiber Optic Source

Great using in the cage, reduced heat and noiseVariable Intensity, Remote Control unit, Heat Filter,Ushio JCR 12V/100WB lamp, 2 meter fiber.

Approximately 1000 hour lamp life

• I Series LH 100 w/o heat filter Requires extension cable & adapter

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IR-DIC and Confocal combinationConfocal microscope C1 & Confo

cal IR-DIC■ Z-motor drive

Motorized Z focus for FN1 will be ready shortly.

■ Merit of compact confocal: C1 Experiment by using FN1 with “compact” C1, is possible even in a small cage. No other competitors confocal microscope offers such system to a bright direct port.

C1 confocal IR-DIC

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Collaboration with NarishigeNew XY mover & isolation stage for FN1

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IR DIC Imaging

IR Polarizer and analyzer sets are available for near infrared Nomarski DIC imaging

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Wide accessories from third party

Combination with Burleigh products

Narishige Micromanipulator

More exciting accessories from Nikonand also from third party will be available.

Thank you

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