MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the Cap-Binding Complex eIF4F...
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Transcript of MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the Cap-Binding Complex eIF4F...
MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the
Cap-Binding Complex eIF4FNahum Sonenberg et al.
Science, September 2007
Presentation byAnsuman sahoo
Advanced Mol. Bio Class07.10.14
Fig. 1. Repression of protein synthesis by let-7 miRNA in an ascites extract.
G Mathonnet et al. Science 2007;317:1764-1767
Published by AAAS
Fig. 2. let-7 miRNA inhibits translation without affecting mRNA stability in an ascites extract.
G Mathonnet et al. Science 2007;317:1764-1767
Published by AAAS
Fig. 3. let-7 miRNA inhibits ribosome binding.
G Mathonnet et al. Science 2007;317:1764-1767
Published by AAAS
Fig. 4. let-7 miRNA inhibits cap-dependent, but not cap-independent, translation in an ascites extract.
G Mathonnet et al. Science 2007;317:1764-1767
Published by AAAS
Figure : Possible Mechanisms of miRISC-Mediated Repression Nonrepressed mRNAs recruit initiation factors and ribosomal subunits and form circularized structures that enhance translation (top). When miRISCs bind to mRNAs, they can repress initiation at the...
Richard W. Carthew , Erik J. Sontheimer
Origins and Mechanisms of miRNAs and siRNAs
Cell, Volume 136, Issue 4, 2009, 642 - 655