Microbubble Generation for Medical Applications
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Transcript of Microbubble Generation for Medical Applications
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Microbubble Generation for Medical Applications
Shirley Zhang
Faculty Mentor: Abraham LeeGraduate Student: Kanaka Hettiarachchi
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Overview
Microfluidic devices for generating bubbles – test for optimal conditions
Create double layer bubbles to contain lipid and oil surrounding gas
Test stability and attachment of bubbles in vitro
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Making Devices
U.V.
Silicon wafer
PhotoresistMask
PDMS
Microfluidic channels
Develop
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Monolayer bubbles
Components: Lipid
(DSPE/DPPE-PEG2000)
Gas (PFC or N2)
Diameter: ~15 um
ColorCam10x magnification
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Fluorescence
DiI mixed into lipids
ColorCam40x magnification
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Double Layer Bubbles
Components: Lipid Oil Gas (PFC or N2)
No lipid shell – no concentrated fluorescence around bubbles
FastCam
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Problems
DSPC/DSPE-PEG2KTriacetin
N2
FastCam
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Future Direction
Successfully make stable double layer bubbles
Microbubbles as a drug delivery system in cell culture