Microbiology Quality Control
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Transcript of Microbiology Quality Control
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Saeeda. ChandioSenior technologist II
Microbiology
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Process or system for monitoring thequality of laboratory testing, and theaccuracy and precision of results
Routinely collect and analyze data fromevery test run or procedure
Allows for immediate corrective action
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IQC refers to the set of proceduresundertaken by the laboratory staff for the
continuousand immediate
monitoring of laboratory work in order todecide whether the results are reliable
enough to be released.
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Quality of specimen Validity of test method Performance of test procedure, reagents
and media Reports and interpretation Staff Equipment
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Lab Tech-The person who performs testing
Supervisor-The person who is responsible forday-to-day activities, training, delegation ofwork
Director-The person who is responsible forentire seamless operation, planning, andcontrol of all activities
Ministry of Health-Place responsible forinfrastructure, man power, and resources.
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Must be trained to perform the tests
Follow SOPs
Must run QC samples
Maintain all the record up to date Inform the Supervisor of any problems---
immediately
Corrective actions taken by them must benoted down and any implications on othersamples
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Train the Lab tech for the assigned job Provide periodic training to the lab techs
Prepare the controls and check the values
Prepare and update the SOPs -easy tounderstand
Keep record of Equipment Maintenance,problems, and repairs
Keep records of training, SOPs, equipment,etc.
Ensure all Lab tasks on time
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The Quality of the specimen
The Performance of test procedures,
reagents and media
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Appropriate specimen
Collection method
Delivery system
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Record amount prepared
Source
Lot number
Sterilization method
Preparation date and Expiration date
Prepared by
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Check:
Sterility
Ability to support growth
Indicative, selective, or inhibitory
Characteristics of the medium
Biochemical response
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Test QC organisms with each new batch orlot number
Check for growth of fastidious organismson media of choice incubate at time andtemp recommended
RECORD Results on Media QC form
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Biological indicators (BIs)
Chemical indicators (CIs)
Mechanical monitoring
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If you have not documented it,
you have NOT done it
If you have not documented,
it is a RUMOUR !!!
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Staining and Microscopy Culture Media
Incubation
Organism Identification Antimicrobial Susceptibility Testing
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Dating when received and when opened Weighing
Dissolving
Sterilizing Storage of prepared media
Addition of enrichments [e.g. Blood]
Sterility Testing
Incubation of inoculated media
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Agar Plates Haemolysis controls
Selective media controls
Liquid broths
Positive and negative reactions
Therefore Stock Cultures
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Organisms to be maintained must beadequate to check all media and test systems.
E. coli MacConkey, XLD, susceptibility
tests Staphylococcus aureus Blood agar,
Mannitol Salt, susceptibility tests Neisseria gonorrhoeae chocolate,
Thayer-Martin
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Gram stain QC Use gram positive and gram negative
organisms to check daily ZN QC
Use positive and negative AFB slide withevery batch stained. Other Stains
Check as used positive and negativereactions
Reagents Check as used with known positives and
negatives.
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Storage of antibiotic disks
CLSI Method
Detailed QC Procedures to ensurereproducibility of results
0.5 Mcfarlands standard
Depth of agar!!
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Many organisms have predictableantimicrobial test results
Staphylococcus spp. are usuallysusceptible to vancomycin
Streptococcus pyogenes are alwayssusceptible to penicillin
Klebsiella pneumoniae are resistant to
ampicillin
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If you encounter an unusual pattern rule out error by checking identification of
organisms
repeat antimicrobial susceptibility test
Report if repeat testing yields same result,or refer the isolate to a reference laboratory
for confirmation
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Clinician should be notified about panicvalues immediately. Panic values are lifepotential-threatening results,
Positive LPA
Positive gram stain for CSF
Positive KLB
Positive blood culture.
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thanks