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FAR 121/4

MICROBIOLOGY FOR PHARMACY

 Practical 3: Bacterial Count

Nae : LOW KAH JUN (127920)

  NAVIN KUMAR A/L THAMILARAJAN (127933)

  FOONG PEI PEI (127899)

  LYDIA ADILA INTI I!KANDAR (127339)

  NUR KHADIJAH INTI A!HARUDIN (1279"#)

Grou! : A

"ate : 19$% NOVEMER 201&

Lecturer : A'P' DR' HJH PAILAH IRAHIM

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O#$ecti%e&

1' T* +,-*., -*.,$,$ ,$- $,-%45,

2' T* ,6*6. +-$,6 -*5$

Intro'uction

I$ *$, ,6+,: ,;, ,,$: $* ;? .-6**6?. 6,

 6,,$ 6$-56 ,;6*.,$' T%,6, 6, ;6*5 .,$%* =%-% - +, 5, $*

,$,6., $%, 5.+,6 * ;+, *6?.: .,>: *56@$, .,$%*: 6,@$, .,$%*:

6*@$5+, .,$%*: 6*@$,B .,$%* (M, M6): .,.+6, $6$* .,$%*: ,$-'

T%, .*$ =,> 5, $,-%45, * ;+, -*5$? 5$* $? ($%, $, -*5$)' W%,

$ % ,;,6 *+, *56-, * ,66*6: $% $,-%45, % 6*;, $* +, $%, .*$ 6,+,:

?;? -*$,$ 6,5$ =$% ., 6$5' E,$>: $%, $,-%45, -*$ * . -5+$* -*5$? * $%,

-**, =%-% %;, ?6*=' F6*. $%, 5.+,6 * -**, =%-% %;, ?6*= $ $%, ;6*5

5$*: $%, $*$ 5.+,6 * -, $%, ., - +, ,$.$,' I $% ,C,6.,$:

$5,$ 6, $6*5-, $* 2 * $%, +*;, .,$%* $* ,$.$, $%, 5.+,6 * ;+, *6?.

$%, ., ?;,'

(A) Pour*!late Met+o'

T% .,$%* -*..*> 5, *6 ;+, -*5$? +,-5, $ ;,6> 6-$-' O, * 

$%, .*6$$ -$*6 -66>? *5$ $%, 5$* $* ,6, > -5.? * -,' I $%

.,$%*: $%, $,.,6$56, $%, ,6* * -5+$* 6, * .*6$$' P,$6 %, $%$

>, 200@300 -**, 6, -*,6, $-$*6>' T% .,$%* .> * +, 5, *6

*$? +-$,6 -5$56,'

Material& R,,6 $* 6-$- .5 * FAR 121/" M-6*+**?> *6 P%6.-> (?, 10)

Proce'ure R,,6 $* 6-$- .5 * FAR 121/" M-6*+**?> *6 P%6.-> (?, 11)

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Re&ult&

D5$* N5.+,6 * +-$,6 -**,

P,$6 D% 1 P,$6 D% 2 A;,6?,

10@"

300˃ 300˃ 300˃

10@& 300˃ 300˃ 300˃

10@# 300˃ 21& 2&7'&

10@7 "1 #8 &"'&

I $%, *56@$, .,$%*: =, ,$,6., $%, 5.+,6 * +-$,6 1'0 . ., * +-$,6

-5$56, $%, ,$6 % =%-% % 6?, * 30 $* 300 +-$,6 -**,' T%, $*$ 5.+,6 *

;+, +-$,6 *6 ,-% ,$6 % --5$,' 10@7 5$* -%*, +,-5, $ -*$

&"'& && +-$,6 -**, ;,6?, $%$ ?;, 6,+, -*5$ * 30 $* 300 -**,' I$

5., $%$ ,-% -**> 6,6,,$ *, +-$,65.'

F*6 $? ;*5., 1'0 .

T*$ 5.+,6 * ;+, +-$,6

A;,6?, $, -*5$ C R,-6*- * 5$* C1

 plating volume

10@7 5$*

T*$ 5.+,6 * ;+, +-$,6 ,6 .

&& C1

10−7  C1

1.0   ,-, . 10 CF/l

(B) !rea'*!late et+o'

  O, *$%,6 => $* ,$,6., +-$,6 -*5$ $* 5, $%, 6,@$, .,$%*'

 N,;,6$%,,: $ %*5 +, *$, $%$ $%, 5, * $%, 6,,6 $% .,$%* $* 6, $%,

*6?. -*5 , $* 6,5-$* $%, $*$ -*5$ $%,6, *+$> $%$ *.,

 +-$,6 -, .?%$ $-< $* $%, 6,,6' J5$ $%, *56 $, .,$%*: $% 6*-,56,

.> * +, 5, $* *$, +-$,6 -5$56,'

Material&: R,,6 $* 6-$- .5 * FAR 121/" M-6*+**?> *6 P%6.-> (?, 11)

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Met+o': R,,6 $* 6-$- .5 * FAR 121/" M-6*+**?> *6 P%6.-> (?, 12)

Re&ult:

D5$* N5.+,6 * +-$,6 -**,

P,$6 D% 1 P,$6 D% 2 A;,6?,

10@" 300˃ 300˃ 300˃

10@& 300˃ 300˃ 300˃

10@# 37 2" 30'&

10@7 33 300

**>@*6.? 5$ (FU/.) 6,6,,$ 5.+,6 * +-$,6 ,6 . * $ -5$56,'

F*6.5 $* --5$, FU/. @A;,6?, 5.+,6 * +-$,6 * $%, $, C 6,-6*- * $%, 5$* C 1/$? ;*5.,

F*6 $% ,C,6.,$: =, - *> --5$, $%, $, =$% 5$* 10 @# *> +,-5, $%,>

6,,6+, 6?, (30 300 -**,)'

 !*: FU/. (37 2")/2 C 1/10@# C 1/0'1 .

  30'& C 10

#

 C 10  3-, . 10CF/l

"i&cu&&ion:

  A,$- $,-%45, =,6, 5, $% ,C,6.,$ $* 6,;,$ +-$,6 -*$.$*

$* 6,;,$ +*6$*6> ,-$* $*=6 $%, +-$,6 -5$56,' A,$- +-> $%,

.,$ *

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.,$%*' W%, 5? 6, $, .,$%* 0'1.L * 5$, +-$,6 ., = 6,

$%, ,$6 %' A$,6 $%$: $%, ,$6 %, 6, -5+$, 5,6 37 *6 "8 %*56' T%,6, 6,

,;,6 $, $%$ =,6, **=, *6 $%, ,$- $,-%45,' T%,, $, =,6, $

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: =, $ 5 $%, * $%, ,$6 % * =, =%, 6,? $%, +-$,6 5,* * $%,

,$6, 56-, * $%, ?6' F*6 $%$: =, %;, $* +, 6,> -6,5 =6, 5, $%, ,$-

$,-%45, 6,-*..,, =%, -*5-$? *56 ,C,6.,$ -, $%,6, *$ * *+$, * 

-*$.$* .> *--56 **6 ,$- $,-%45, +,? ,'

T%, 5,;, 6,5$ * .> +, -5, +> $%, .$ 5, =*6=, *6 ;+, -*5$? +,-5, $ ;,6>

 6-$-' I$ % $%, ;$?, * *$ 6,456? 6,;*5> 6,6, $,: *$, 5, $*

> +-$,6 -*$.$* **' T%, ;$?, * 5? *56 $, $%, 5.$*

$%$ ,-% -**> 6, 6*. ,-% -,' T%, $,.,6$56, .5$ +, *$., $* -%,;, $%,

6?,$ -**, *+, * $%$ $ ,> *6 -*5$?' T%, ;$?, * $% .,$%* $%$

=, - *+,6;, =%,$%,6 $%, +-$,6 ,6*+-: ,6*+- * -5$$;, ,6*+-' I *56

 $,: +-$,6 = +, $6, $%, ?6 $%, ,6*+, -5$$;, ,6*+, = ?6*=

*> =$% $%, ?6 .,'

H*=,;,6: $%,6, 6, ,= ;$?, * 6,@$, .,$%* -*.6, $* *56@

 $, .,$%* *6 ,6*6.? ;+, -*5$ * +-$,6' F6$>: *., * $%, +-$,6 .> $-<

$* $%, 6,,6 $% = 6,5-, $%, 5.+,6 * +-$,6 -*5$,: $%5 $%, 5.+,6 *

 +-$,6 -*5$, = +, --56$,' N,C$: *., +-$,6 .?%$ +, $%, -*%* * $%,

6,,6' ,,: 6*6$, $,-%45, * 6,? =*5 6,5$ -5.? * +-$,6

-**,'

Conclu&ion:

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W, - ,$.$, $%, 5.+,6 * ;+, *6?. $%, ., ?;, -,> +> -66>? *5$

6, $, *6 *56 $, .,$%*' T%, 5.+,6 * +-$,6 -, ,6 .L * ., - +,

,$,6., +> -66> *5$ +-$,6 -*5$' *$% * $%,, .,$%* 5, $%, ,6 5$*

.,$%* $* 6,5-, $%, $*$ 5.+,6 * +-$,6 * $%$ $%,> 6, -*5$+,' T%, 5.+,6 * 

 +-$,6 ,6 . * ., - +, ,$.$, +> $%, **=? *6.5

T%, 5.+,6 * +-$,6 A;,6?, $, -*5$ C 1/5$* -$*6 C 1/$?

;*5.,

Reerence&:

1' J R,>*' !,6 D5$* P6*$*-*' A!MM-6*+,L+66>'L$ 5$, * 1$ A6

2013' R,$6,;, * 31$ O-$*+,6 201&' A;+, 6*.

%$$//==='.-6*+,+66>'*6?/-*.*,$/6,*56-,/+*6$*6>@$,$/288"@,6@

5$*@6*$*-*

2' !6,@P$, T,-%45,' $, * 1$ N*;,.+,6 201&' A;+, 6*.

%$$//%>'%5',5'-//%>/10'%$.

3' Q5$>? -$,6 +> !6, P$,' M-6*+**?> T,-%45,' $, * 1$ 

 N*;,.+,6 201&' A;+, 6*.

%$$//===2'%,6C',5/+**?>/,W,+/T,-%45,/.-6*6,'%$.

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