Microbiology Lab Report 2

8/17/2019 Microbiology Lab Report 2

1/13

FAR 121/4

MICROBIOLOGY FOR PHARMACY

PRACTICAL REPORT 2:

A) EXAMINATION OF CULTURES FOR MORTALITY

B) CULTURE TECHNIQUE

Name : Low Kah Jun

Ma!"# No : $%&'%(

No IC : '*(+%*,(*,++%'

-ae : +h O.o/e! %($+

Le.u!e! : A00o. 1!o2 -! H3h 1a4"5ah I/!ah"m


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I. EXAMINATION OF CULTURES FOR MOTILITY

Objectives:

$ To "6en"27 an6 6"22e!en"ae /eween !ue mo"5"7 an6 B!own"an mo"on% To 2am"5"a!"4e an6 8e!2e. he"! a0e8". e.hn"9ue0 "n

, han65"n a0e8". !an02e!0

, "0o5a"n a 8u!e .u5u!e

Intro!ction:

A 5a!e num/e! o2 m".!oo!an"0m0 a!e mo!e o! 5e00 a.";e57 mo"5e /7 25ae55a

F5ae55a a!e 5on h!ea6,5" 8ae +

Met$o:

%A&'"n(in( )ro* *re*"r"tion

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae *

%+& So,t "("r -st"b

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae *

Res!#t:

%A& '"n(in( ro* *re*"r"tion


3/13

O/0e!;a"on0 E#85ana"on

mo"on o2 Pseudomonas aeruginosa

Pseudomonas aeruginosa

Man"2".a"on : @( $(

•

Ro6,0ha8e6 Pseudomonas aeruginosamo;e0 "n a 4"4ae6,5"


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Pseudomonas aeruginosa

•

!owh o2 Pseudomonas aerugina "008!ea6 on he 0u!2a.e o2 he 0o2 aa!

an6 he 0"n5e 0a/ 5"ne D5"ne o2

"no.u5a"on) ma6e /7 he 0!a"h w"!e

• Ba.e!"a !owh a!ea "0 5a!e!

• Pseudomonas aerugina "0 a mo"5e

/a.e!"a

Staphylococcus aureus

• !owh o2 Staphylococcus aureus "0

5"m"e6> 3u0 a!oun6 he 0"n5e 0a/

ma6e /7 he 0!a"h w"!e on he 0o2

aa!

• The 0a/ 5"ne "0 ;e!7 0ha!8 an6 6e2"ne0

• Ba.e!"a !owh a!ea "0 0ma55e!

• Staphylococcus aureus "0 a non,mo"5e

/a.e!"um

)isc!ssion:

DA) Han"n -!o8 Meho6

Mo"5"7 o2 m".!oo!an"0m "0 one o2 he meho60 ha u0ua557 u0e6 o 0how0 he 8!e0en.e

o2 he 25ae55a "n /a.e!"a Fo! e#am85e0 0o2 aa! 0a/ meho6> we,moun e.hn"9ue an6


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han"n 6!o8 8!e8a!a"on an6 o/0e!;a"on un6e! he m".!o0.o8e Th"0 meho6 6oe0 no ";e

he 6ea"50 "n amoun an6 a!!anemen o2 he 25ae55a /u u0e6 o 0how he 8!e0en.e o2

25ae55a The mo"5"7 .an /e 6";"6e6 "no h!ee 78e0 a0 /e5ow:

$ 0how !ue mo"5"7 /7 mo;"n "n a 6e2"n"e 6"!e."on% /!own"an mo;emen 6ue o he .o55"0"on0 w"h 0u!!oun6"n 8a!".5e0

G non,mo"5"7 /7 ;"/!a"n on he 0ame 08o

A2e! he han"n 6!o8 8!e8a!a"on> he 05"6e a!e 8u on he 0ae o2 m".!o0.o8e>

Pseudomonas aeruginosa 0how0 he mo;emen "n 0ame an6 6e2"n"e 6"!e."on Th"0 8!o;e6

ha Pseudomonas aeruginosa 8o00e00 25ae55a o a"6 "n mo;emen The!e2o!e> he7 0how !ue

mo"5"7 B7 u0"n he man"2".a"on o2 @((> mo"5"7 o2 he Pseudomonas aeruginosa .an /e

o/0e!;e6

Fo! Staphylococcus aureus> " "0 mo;"n "n 6"22e!en "n6e2"n"e 6"!e."on0 an6 0ome

;"/!a"n ;"o!ou057 on he 0ame 08o Th"0 .a55e6 B!own"an mo;emen an6 "0 6ue o he

.o55"0"on o2 he /a.e!"a w"h he 0u!!oun6"n 8a!".5e0 5"


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% A0e8". e.hn"9ue0 0hou56 /e .a!!"e6 ou 6u!"n he e#8e!"men

G The 5"h "nen0"7 o2 he m".!o0.o8e 0hou56 /e m"n"m"4e6 o 8!e;en he /a.e!"a 2!om

6!7"n ou an6 67"n

@ The .u5u!e /!oh 0hou56 /e en57 0ha han"n 6!o8 meho6 nee60 a h"he! 0 Pseudomonas aeruginosa "0 a mo"5e /a.e!"a 6ue o he 8!e0en.e o2 25ae55a

Staphylococcus aureus "0 a non,mo"5e /a.e!"a

II. CULTURE TEC'NI/UE

Intro!ction:

A0 mo0 m".!oo!an"0m0 a!e 8oen"a557 8ahoen". " "0 e00en"a5 ha he7 /e han65e6

/7 8!o.e6u!e0 wh".h a;o"6 .onam"na"on o2 he m".!o/"o5o"0 A50o 0"n.e m".!oo!an"0m0


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a!e 0o u/"9u"ou0> " "0 ne.e00a!7 o a;o"6 .onam"na"on o2 m".!o/"a5 .u5u!e /7 0!a7

m".!oo!an"0m0

0& Inoc!#!1 tr"ns,er

"& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 one "("r *#"te to "not$er b3 stre"4in(

M"teri"#s:

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae &

Met$o:

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae &

b& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 "n "("r *#"te to " bott#e o, n!trient brot$

M"teri"#s:

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae

Met$o:

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae

Res!#t:

0& Inoc!#!1 tr"ns,er

"& Tec$ni!e o, tr"ns,errin( c!#t!re ,ro1 one "("r *#"te to "not$er b3

stre"4in(



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Escherichia coli

D "n aa! 85ae)

• The .o5on"e0 o2 Escherichia coli

a!e 08!ea6 2!om he 2"!0 0!ea


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o @ hou!0 -"22e!en /a.e!"a ha;e 6"22e!en .o5ou!0> e#u!e0> 0ha8e0 an6 0"4e0 The

.o5on"e0 o2 Escherichia coli a!e !oun6 "n 0ha8e an6 m"5 0o he 0!ea


10/13

Re2e! o FAR $%$=@ MICROBIOLOY FOR 1HARMACY 8!a.".a5 manua5> 8ae '

Res!#t:

"& Fro1 " 1i5e c!#t!re *#"te to " *"rtitione "("r *#"te


M"#e6 .u5u!e o2 Bacillus subtilis an6

Staphylococcus aureus .o5on"e0

• M"#e6 .u5u!e o2 Bacillus

subtilis an6 Staphylococcus

aureus.

• The .o5on"e0 o2 Bacillus

subtilis a!e 08he!".a5 "n 0ha8e>0ma55e! an6 wh"e D.!eam7) "n

.o5ou!

• The .o5on"e0 o2

Staphylococcus aureus a!e

0ma55> !oun6e6> 7e55ow an6

m"5


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A2e! "no.u5a"on

Bacillus subtilis an6 Staphylococcus aureus

• Be2o!e "n.u/a"on> he nu!"en

/!oh wa0 .5ea! 6ue o a/0en.e o2

/a.e!"a5 !owh• A2e! "n.u/a"on> he nu!"en /!oh

u!ne6 .5ou67 an6 u!/"6 6ue o

/a.e!"a5 !owh an6 mea/o5"e o2

Bacillus subtilis an6

Staphylococcus aureus.

• T!an02e! o2 /a.e!"a wa0 6one

0u..e002u557

)isc!ssion:

S!ea


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% Su22"."en "me mu0 /e ";en o .oo5 he w"!e 5oo8 /e2o!e han65"n /a.e!"a o 8!e;en

/a.e!"a 6eah /7 hea

G E;e!7 0!ea !oun6e6> m"5 /u " "0 no 0u"a/5e 2o! "0o5a"on o2 /a.e!"a a0 a55 he

.o5on"e0 w"55 /e m"#e6 "n he /!oh Th"0 e.hn"9ue wa0 0u..e002u557 8e!2o!me6

Res!#ts:

Part 1

a. Observe the streaked plate and make a sketch to show the distribution of growth.

15ea0e !e2e! o he o/0e!;a"on o2 "no.u5um !an02e! "n e.hn"9ue0 o2 !an02e!!"n .u5u!e 2!om

one aa! 85ae o anohe! /7 0!ea he nu!"en /!oh "0 .5ea! an6 !an08a!en an6 " /e.ome0 u!/"6

a2e! "no.u5a"on Th"0 "0 6ue o he !owh o2 Escherichia coli "n he nu!"en /!oh

an6 he /a.e!"a 0e.!ee wa0e 8!o6u.0 o2 mea/o5"0m 6u!"n "0 !owh


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6 !an you use a li"uid medium to isolate a pure culture? E#plain.

No> we .anno u0e a 5"9u"6 me6"um o "0o5ae a 8u!e .u5u!e /e.au0e " "0 no a 0u"a/5e

meho6 e .anno 6ee!m"ne whehe! he u!/"6"7 o2 he /!oh "0 .au0e6 /7 a 8u!e

.u5u!e o! he .onam"na"on o2 ohe! m".!oo!an"0m0

Part 2

a. Observe the streaked plate. Were you able to separate the $ organisms successfully? %f

not& why? 'escribe the morphology of the colonies produced( )colour& te#ture& shape

etc.

The m"#e6 .u5u!e o2 Bacillus subtilis an6 Staphylococcus aureus a!e 0u..e002u557

0e8a!ae6 The .o5on"e0 o2 Bacillus subtilis a!e 08he!".a5 "n 0ha8e> 0ma55e!> 0mooh "n

a88ea!an.e an6 wh"e D.!eam7) "n .o5ou!> wh"5e he .o5on"e0 o2 Staphylococcus

aureus a!e 08he!".a5 "n 0ha8e> /"e!> !ouh "n a88ea!an.e an6 7e55ow"0h "n .o5ou!

/ *ow would you know if your culture has been contaminated?

e w"55 he wae! 6!o85e0 w"55 6!"8 ono he 0u!2a.e o2aa! Th"0 w"55 a22e. he 2o!ma"on o2 .o5on"e0 o2 /a.e!"a /e.au0e he wae! w"55 .au0e he

/a.e!"a .o5on"e0 o /"n6 oehe! a0 a me6"um 2o! mo;emen o2 /a.e!"a> 5ea6"n o an

"na..u!ae !e0u5

Microbiology Lab Report 2

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Transcript of Microbiology Lab Report 2