MICROBIOLOGY

COSMETICS

The SOLABIA group

Founded in 1972, SOLABIA is specialized in the manufacturing of ingredients and

actives for cosmetic, pharmaceutical and biotechnological industries.

Major actor in the field of cosmetic, SOLABIA develops actives and concepts-formulae

for major cosmetic brands.

Trough BIOKAR Diagnostics, SOLABIA is a major player in microbiology.

Leader of peptone manufacturing, SOLABIA supplies principals cultural medium

manufacturing in Europe.

With 350 collaborators in the worldwide, the group is comprised of 5 manufacturing

sites and 3 R&D centers.

BIOKAR Diagnostics develops solutions for microbiological control in :

Foodstuffs

Water and environment

Cosmetic

Animal health

Our portfolio is composed of more than 300 ready-to-use and dehydrated culture

media, and associated supplements.

Our expertise in microbiology is well known in more than 40 countries.

BIOKAR Diagnostics

BIOKAR Diagnostics

Our cosmetics portfolio

Tryptone-salt broth

50 tubes of 9 mL - BM00808 10 vials of 90 mL - BM11408

Eugon LT 100 broth

50 tubes of 9 mL - BM00608 50 tubes of 9 mL with glass beads - BM17508 10 vials of 100 mL - BM04308

Diluent and suspension

Permits a high level of microbial resuscitation

Tryptone-salt broth

Diluent and enrichment medium for all type of micro-organisms

Contains neutralizers to offset preservatives

Eugon LT 100 broth

Diluent/neutralizer

1 g or 1 mL of 1/10 diluted samples

Eugon LT 100 Broth

SABOURAUD Dextrose Chloramphenicol agar

Enumeration of yeasts and molds

3 to 5 days at 25 ± 2.5°C

NF EN ISO 16212 : 2011-08 SABOURAUD Dextrose Chloramphenicol agar (SDCA)

Growth of yeasts and molds

Inhibition of bacterial growth

SABOURAUD Dextrose Chloramphénicol Agar (SDCA)

20 Plates - BM17608 120 Plates - BM17708 10 vials of 200 mL - BM17208

Enumeration of yeasts and molds

Eugon LT 100 broth

Trypto-casein soy agar

Enumeration

72 ± 6 h at 32.5 ± 2.5°C

Eugon LT 100 agar

at least 20 h At 32.5 ± 2.5°C

48 to 72 h at 32.5 ± 2.5°C

Presence/ Absence

NF EN ISO 21149 : 2009-09

Trypto-casein soy agar (TSA)

20 Plates - BM05008 120 Plates - BM17808 10 vials of 100 mL - BM01708 10 vials of 200 mL - BM04908

Eugon LT 100 agar

20 Plates - BM17008 10 vials of 200 mL - BM04508

Trypto-casein soy agar (TSA)

Nutritive agar for all types of microorganisms

Eugon LT 100 agar

Nutritive agar

Neutralization of the antimicrobial activity of preservatives

Enumeration and detection of aerobic mesophilic bacteria

1 g or 1 mL of 1/10 diluted samples

Eugon LT 100 broth

Trypto-casein soy agar

24 to 48 h at 32.5 ± 2.5°C

Identification of micro-organisms :

Escherichia coli

Pseudomonas aeruginosa

Staphylococcus aureus

Candida albicans

Non-specified micro-organisms

20 to 72 h at 32.5 ± 2.5°C

NF EN ISO 18415 : 2011-08

Trypto-casein soy agar (TSA)

20 Plates- BM05008 120 Plates - BM17808 10 vials of 100 mL - BM01708 10 vials of 200 mL - BM04908

Trypto-casein soy agar (TSA)

Nutritive agar for all typed of microorganisms

Conforms to the formula described in European and

American Pharmacopoeia

Detection of specified and non-specified micro-organisms

1 g or 1 mL of 1/10 diluted samples

Eugon LT 100 broth

SABOURAUD Dextrose Chloramphenicol agar

24 to 48 h at 32.5 ± 2.5°C

Identification tests

20 to 72 h at 32.5 ± 2.5°C

NF EN ISO 18416 : 2009-09

SABOURAUD Dextrose Chloramphenicol Agar (SDCA)

20 Plates - BM17608 120 Plates - BM17708 10 vials of 200 mL - BM17208

SABOURAUD Dextrose Chloramphenicol agar (SDCA)

Growth of yeasts and molds

Inhibition of bacterial growth

Detection of Candida albicans

Candida albicans

Characteristic colonies white creamy color

1 g or 1 mL of 1/10 diluted samples

Eugon LT 100 broth

MacCONKEY agar

24 to 48 h at 32.5 ± 2.5°C

Identification tests

20 to 72 h at 32.5 ± 2.5°C

NF EN ISO 21150 : 2009-09

MacCONKEY agar

10 vials of 100 mL - BM18008

MacCONKEY agar

Isolation of Enterobacteria such as E. coli

Corresponds to the formula described in European and

American Pharmacopoeia

Escherichia coli

Characteristic colonies Metallic sheen color under reflected light and a blue-black appearance under transmitted light

1 g or 1 mL of 1/10 diluted samples

Detection of Escherichia coli

Eugon LT 100 broth

Cetrimide agar

24 to 48 h at 32.5 ± 2.5°C

Identification tests

20 to 72 h at 32.5 ± 2.5°C

NF EN ISO 22717 : 2009-09

Cetrimide agar

500 g vial - BK049HA

Cetrimide agar

Isolation of P. aeruginosa.

Conforms to the formula described in European and

American Pharmacopoeia

Pseudomonas aeruginosa

Characteristic colonies Yellow-green color (pyocyanin), which fluoresces under UV light

Detection of Pseudomonas aeruginosa

1 g or 1 mL of 1/10 diluted samples

Eugon LT 100 broth

BAIRD-PARKER agar

24 to 48 h at 32.5 ± 2.5°C

Identification tests

20 to 72 h at 32.5 ± 2.5°C

NF EN ISO 22718 : 2009-09 BAIRD-PARKER agar

Isolation of S. aureus.

Conforms to the formula described in European and

American Pharmacopoeia

BAIRD PARKER agar

20 Plates - BM01808 120 Plates - BM09108

Staphylococcus aureus

Characteristic colonies Black, shiny colonies, surrounded by clear zones (2 mm to 5 mm)

Detection of Staphylococcus aureus

1 g or 1 mL of 1/10 diluted samples

Evaluation of the antimicrobial protection of a cosmetic product - Challenge test

Trypto-casein soy agar (TSA)

20 Plates - BM05008 120 Plates - BM17808 10 vials of 100 mL - BM01708 10 vials of 200 mL - BM04908

SABOURAUD Dextrose agar (SDA)

20 Plates - BM17308 120 Plates - BM17408 10 vials of 200 mL - BM05308

POTATO Dextrose agar (PDA)

500 g vial - BK095HA

30 ± 15 min at room temperature

TSA SDA PDA

48 to 72 h at 32.5 ± 2.5°C

3 to 5 days at 22.5 ± 2.5°C

Enumeration of total bacteria

Enumeration of Candida albicans

Enumeration of Aspergillus brasiliensis

22.5 ± 2.5 °C

20 g or 20 mL of formulation + 0.2 mL of calibrated inoculum

At each specified sampling interval take 1 mL or 1 g of inoculated formulation

7 days : T7 14 days : T14 28 days : T28

Add 9 mL of Eugon LT 100 broth to each samples.

NF EN ISO 11930 : 2012-06

Agars

Pre-poured

In Petri dishes - Pack of 120 - Pack of 20

Ready-to-melt

In vials - 10 x 100 mL - 10 x 200 mL

Dehydrated

In vials - 500 g - 5 kg

Our presentations

Broths

Ready-to-use Dehydrated

In vials - 500 g - 5 kg

In vials - 10 x 90 mL - 10 x 100 mL - 10 x 200 mL

In tubes - 50 x 9 mL - 50 x 10 mL

In flexible bags - 3 x 3 L - 2 x 5 L

Our presentations

Product Presentation BAIRD-PARKER agar BM01808 - 20 Petri dishes Ø 90 mm

BM09108 - 120 Petri dishes Ø 90 mm

Cetrimide agar BK049HA - Vial 500 g

CHAPMAN agar BK030HA - Vial 500 g

Peptoned water BM16408 - 3 flexible bags 3 L

EMB (LEVINE) Agar BK046HA - Vial 500 g

Eugon LT 100 broth BM00608 - 50 tubes 9 mL BM17508 - 50 tubes 9 mL + glass beads BM04308 - 10 vials100 mL

Eugon LT 100 agar BM17008 - 20 Petri dishes Ø 90 mm BM04508 - 10 vials200 mL

Poteto Dextrose agar (with antibiotics) BK095HA - Vial 500 g BS02108 - 10 vials Chloramphenicol 50 mg

MacCONKEY agar BM18008 - 10 vials100 mL

Lactose broth BK082HA - Vial 500 g

SABOURAUD Dextrose agar (SDA) BM17308 - 20 Petri dishes Ø 90 mm BM17408 - 120 Petri dishes Ø 90 mm BM05308 - 10 vials200 mL

SABOURAUD Dextrose Chloramphenicol agar (SDCA)

BM17608 - 20 Petri dishes Ø 90 mm BM17708 - 120 Petri dishes Ø 90 mm BM17208 - 10 vials200 mL

Tryptone salt broth BM00809 - 50 tubes 9 mL BM11408 - 10 vials90 mL BM13508 - 3 poches souples 3 L

Trypto-casein soy broth (TSB) BM03008 - 50 tubes 10 mL BM00908 - 10 vials100 mL BM17908 - 10 vials90 mL

Trypto-casein soy agar (TSA) BM05008 - 20 Petri dishes Ø 90 mm BM17808 - 120 Petri dishes Ø 90 mm BM01708 - 10 vials100 mL BM04908 - 10 vials200 mL

Our presentations

Our controls certify the performance of our products in strict

conformity with ISO standards :

Performance tests

Conditions of transport

Sterility tests

Neutralization tests

Analysis of physical characteristics (aspect, pH...)

BIOKAR Diagnostics quality

BIOKAR Diagnostics is certified according to ISO 9001:2008 quality management standards.

We propose a direct access to technical documents on our web site :

Technical Data Sheets

Safety Data Sheets

Quality Control Certificates

Accessible on our web site : www.biokar-diagnostics.com

BIOKAR Diagnostics - support and services