microbial ecology

13
A Study of Mixed Continuous Cultures of Sulfate-Reducing and Methane-Producing Bacteria TH. E. CAPPENBERG Limnological Institute "Vijverhof," Nieuwersluis, The Netherlands Abstract Ecological relationships between sulfate-reducing and methane-producing bacteria in mud of Lake Vechten have been studied by continuous culture studies using the chemostat technique. The maximum specific growth rate (/-~max) and saturation constant (K~) were, respectively, 0.36 hr -1 and 0.047 mM for lactate-limited growth of Desulfovibrio desulfuri- cans and 0,011 hr -~ and 0.17 mM for acetate-limited growth of Methanobacterium sp. Calculated values for the true molar growth yields(Ya ) and maintenance coefficients (m) were 30.6 g bacterial mass/mole of lactate and 0.53 g substrate/g dry wt hr for D.desulfuricans and 37.8 g bacterial mass/mole of acetate and 0.54 g substrate/g dry wt hr for Methanobacterium. No growth of Methanobacterium was observed at a pS z- value (the hydrogen sulfide potential) of more than 11 and there was no effect on the growth atpS 2- values above 13. In mixed continuous culture experiments the concentration of acetate decreased in the second- stage growth vessel, whereas that of methane increased stoichiometrically. If the substrate concentration in the reservoirs (St) was increased from 0.1 to 0.5 mg/ml, the population of Desulfovibrio increased and that of Methanobacterium was washed out of the culture vessel, since the concentration of hydrogen sulfide reached a pS 2- value of 10.5. From the mixed continuous culture experiments a commensalism between the two species can be described, i.e., the acetate-fermenting Methanobacteriura benefits from the acetate released by Desul- fovibrio which is, in turn, not affected in the presence of the former. Introduction Previous investigations indicated an ecological interrelationship bet- ween sulfate-reducing and methane-producing bacteria in mud of Lake Vechten [4-6]. It was demonstrated that maximum numbers of the two groups occur at different layers in the bottom deposits. It was suggested that this was due to sensitivity of methane producers to hydrogen sulfide pro- duced by sulfate-reducing bacteria. A substrate relationship between the two groups of bacteria was proposed, since acetate produced by sulfate reducers may be subsequently used by methane producers. Further data on this relationship can be obtained from continuous culture studies. The chemostat provides a model system which can be 6O MICROBIAL ECOLOGY, Vot. 2, 60-72 (1975) 1975 by Springer-Verlag New York Inc.

Transcript of microbial ecology

Page 1: microbial ecology

A Study of Mixed Continuous Cultures of Sulfate-Reducing and Methane-Producing Bacteria

T H . E . C A P P E N B E R G

Limnological Institute "Vijverhof," Nieuwersluis, The Netherlands

Abstract

Ecological relationships between sulfate-reducing and methane-producing bacteria in mud of Lake Vechten have been studied by continuous culture studies using the chemostat technique. The maximum specific growth rate (/-~max) and saturation constant (K~) were, respectively, 0.36 hr -1 and 0.047 mM for lactate-limited growth of Desulfovibrio desulfuri- cans and 0,011 hr -~ and 0.17 mM for acetate-limited growth of Methanobacterium sp. Calculated values for the true molar growth yields(Y a ) and maintenance coefficients (m) were 30.6 g bacterial mass/mole of lactate and 0.53 g substrate/g dry wt hr for D.desulfuricans and 37.8 g bacterial mass/mole of acetate and 0.54 g substrate/g dry wt hr for Methanobacterium.

No growth of Methanobacterium was observed at a pS z- value (the hydrogen sulfide potential) of more than 11 and there was no effect on the growth a t p S 2- values above 13. In mixed continuous culture experiments the concentration of acetate decreased in the second- stage growth vessel, whereas that of methane increased stoichiometrically. If the substrate concentration in the reservoirs (St) was increased from 0.1 to 0.5 mg/ml, the population of Desulfovibrio increased and that of Methanobacterium was washed out of the culture vessel, since the concentration of hydrogen sulfide reached a pS 2- value of 10.5. From the mixed continuous culture experiments a commensal i sm between the two species can be described, i .e. , the acetate-fermenting Methanobacteriura benefits from the acetate released by Desul-

fovibrio which is, in turn, not affected in the presence of the former.

Introduction

Previous investigations indicated an ecological interrelationship bet- ween sulfate-reducing and methane-producing bacteria in mud of Lake Vechten [4 -6] . It was demonstrated that maximum numbers of the two groups occur at different layers in the bottom deposits. It was suggested that this was due to sensitivity of methane producers to hydrogen sulfide pro- duced by sulfate-reducing bacteria. A substrate relationship between the two groups of bacteria was proposed, since acetate produced by sulfate reducers may be subsequently used by methane producers.

Further data on this relationship can be obtained from continuous culture studies. The chemostat provides a model system which can be

6O

MICROBIAL ECOLOGY, Vot. 2, 60 -72 (1975) �9 1975 by Springer-Verlag New York Inc.

Page 2: microbial ecology

Mixed Continuous Cultures of Bacteria 61

a p p l i e d w i d e l y in e c o l o g i c a l a n d p h y s i o l o g i c a l s t u d i e s ( f o r a r e c e n t r e v i e w ,

s ee V e l d k a m p a n d J a n n a s c h [ 1 8 ] ) . I f d i f f e r e n t g r o w t h - l i m i t i n g s u b s t r a t e s

a re u s e d b y d i f f e r e n t o r g a n i s m s , c o e x i s t e n c e w o u l d o c c u r , s i n c e t h e s e

o r g a n i s m s t h e n o c c u p y d i f f e r e n t e c o l o g i c a l n i c h e s . T h e i n f l u e n c e of the

a c e t a t e - p r o d u c i n g b a c t e r i u m , Desul fov ibr io desu l fur icans , o n the f e r m e n t a -

t i on o f l i m i t i n g a m o u n t s o f a c e t a t e b y a m e t h a n e - p r o d u c i n g b a c t e r i u m ,

Methanobac ter ium sp . , w a s i n v e s t i g a t e d in m i x e d c o n t i n u o u s c u l t u r e s . A

c a s e o f c o m m e n s a l i s m thus d i s c o v e r e d is d e s c r i b e d . T h i s s i t u a t i o n i s ,

h o w e v e r , c o m p l i c a t e d b y t h e r e l e a s e o f h y d r o g e n s u l f i d e b y

D.desu l fu r i cans , w h i c h i n h i b i t e d g r o w t h o f t he o t h e r o r g a n i s m .

Methods and Materials

Microorganisms and Media. A Desulfovibrio desulfuricans strain, isolated from mud of Lake Vechten, was used throughout this study. Enrichment and isolation of sulfate- reducing bacteria were performed according to the methods of Postgate (1965). The organism was identified as Desulfovibrio desulfuricans according to the criteria proposed by Campbell and Postgate [12]. Contamination tests for aerobes and anaerobes were performed by the methods described by Postgate [11]. The liquid medium was a modification of that used previously [3], that is, by omitting Fe(NH4)2SO4 and additing Na-ethylenediaminetetra- acetate (0.0005 % v/v) as chelating agent.

A Methanobacterium sp. was isolated from the mud of Lake Vechten according to enrichment methods proposed by Barker [2]. The inoculum was transferred to a glass- stoppered bottle, completely filled with the medium as described by the author in a recent paper [4] and incubated at 30~ for appropriate periods. Organisms from this enriched culture on acetate were isolated by serial dilution according to the roll-tube technique of Hungate [8] with an agar medium under an oxygen-free N2 atmosphere. A strain was obtained as a colony from the highest dilution (10 -r) showing growth and methane produc- tion. The organism produced methane from acetate and from hydrogen and carbon dioxide. The organism was a nonmotile, nonsporeforming, Gram-variable rod and was not indentical to the types of methane bacteria described by Wolfe [19] and Zeikus and Wolfe [20]. The strain, however, resembled that in the enrichment culture of Pretorius [13] using acetate as sole energy source. Morphologically, these bacteria were identical to the rod-shaped bacteria found by Mylroie and Hungate [9] in the acetate-enrichment cultures.

Lactate and acetate were added to the media in the concentrations as indicated in the text.

Apparatus. Single stage continuous culture experiments were performed using a Mic- roferm Fermentor and Continuous Culture Console (New Brunswick Scientific Co., Inc., New Brunswick, U.S.A. ). A 2-liter growth vessel made of Pyrex glass and having three extra ports for inserting electrodes was used. Autoclavable glass and gold electrodes (Leeds & Northrop Company, U.S.A.) were used, respectively, to measure the pH and redox potential. The pH and redox potential were measured simultaneously employ'ing an autoclavable calomel refer- ence electrode with a pH- and redox-transminer (Electrofact N.V., the Netherlands). The transmitter is equipped with two current alarm units, which can be preset at the desired values of pH and redox potential in the growth vessel, and with solenoid valves (Radiometer type MNV 2, Denmark) to deliver HCI (0.02 N), NaOH (0.02 N), perchloric acid (0 .1% v/v), and Na~S'9 H20 (0. l % v/v) in the vessel. A two-pen potentiometric recorder was used for continuous registration.

Page 3: microbial ecology

62 Th. E. Cappenberg

The apparatus for mixed culture experiments consisted of three all-glass, water-jacked culture vessels fitted with multi-socked flat-flanged lids and Quick-fit glass components (as proposed by Baker [1]) . Glass tubing was preferably used, and connections were made with heavy-wall butyl rubber tubing. The operating volume in the vessels could be controlled by adjusting the overflow. The fl~w rates between the reservoirs and the culture "vessels were controlled by peristaltic pumps (Vario Perpex 12000, LKB Producten AB, Sweden). Seven sockeds in the lid provided connections to the reservoirs and the buffering solutions, and fitted an overflow, an inoculation and sampling tube, electrodes, and gas inlet. Electrodes were autoclavable combined glass and reference electrodes (Radiometer, Denmark or Leeds & Northrop, U.S.A.). Redox- and pS z electrodes were designed and calibrated as described previously [4]. By simultaneous measurements of the pH and thepS 2 (the hydrogen sulfide potential) the H~Sg,~ concentration can be calculated from the equilibrium constant for the

reaction H-,S~,,s -~- 2 H+aq + S~.a~. = 10 ~z~'~

The methane-producing bacteria were cultured at constant pH and redox potential, and the sulfate reducing bacteria at constant pH. In mixed cultures only the redox potential was kept constant. The pH could not be kept constant but was fbund not to change significantly. ThepS z both in culture of sulfate-reducing bacteria and in mixed culture was measured with a pS z electrode. No "poisoning" of the electrodes by culture constituents has been noted by runs lasting a few months. The electrodes were connected to a Titrator TTT 2 (Two-channel Titrator module + Two way pH-stat module, Radiometer, Denmark). Meter and electrode drifts were corrected, if necessary, after checking the values of the routine dally samples externally. Each fermentor was connected to a nitrogen tank, the pressure being maintained at 1.1 aim, and was stirred by a Teflon-covered magnet. Traces of oxygen in the nitrogen gas were removed by leading it through a column of BASF catalyzer R3- 11 at 150~ The gas was sterilized by a micro-flow filter (Microflow Ltd., England). All liquids were thoroughly treated with this gas before they were added to the system. Resazurin in the medium remained

reduced throughout the experiments.

The apparatus was maintained at a temperature of 30 ~ -+ 0.01"C with a thermostat (TE 9, P.M. Tamson N.V., the Netherlands). The glassware, tubes, and liquids were sterilized by autoclaving (20 rain, 118~ except those for the energy and carbon sources, which were sterilized by filtration through a Sartorius filter (0.22/z). If wall-growth occurred in the vessels with Methanobacterium after runs of over 100 days, as happened occasionally, the experiment was immediately suspended. The rate at which the buffering solutions was added to the cultures was about 4% of the medium flow rate; dilution rates are given as the actual medium flow rate. For theoretical considerations on continuous cultures and for symbols used, reference is made to Tempest [17].

Analyses. The concentrations of acetate and lactate in supernatant fluids of the cultures were assayed spectrophotometrically by specific enzymatic methods as described by Cappen-

berg [5].

Sulfide was determined colometrically using the N,N-dimethyl-p-phenylenediamine method and sulfate turbidimetrically with a 30% BaCIz " 2 H20 solution as proposed by van

Gemerden [7].

The concentration of methane in the gas phase of the cultures was assayed using gas

chromatography [3].

The bacterial dry weight was determined by filtering 10 ml samples through a pre- washed, dried, and preweighed Sartorius membrane filter (0.22/.t). The sample was rir~sed

twice with 5 ml membrane-filtered water, and dried at 115~ for I hr and weighed.

Page 4: microbial ecology

Mixed Continuous Cultures of Bacteria 63

Bacterial concentrations are expressed either as units of optical density (2 cm cuvet, filter Hg 365, Zeiss Elko lid or as numbers of cells per milliliter obtained by differential counts of Desulfovibrio and Methanobacterium by microscopic examination (Biirker-Tiirk counting chamber, depth 0.01 ram).

The chemicals used were all of reagent grade quality and obtained from commercial sources.

Results

Batch Culures. The doubl ing time (t a) and specif ic growth rate (/x)

of Desulfovibrio and Methanobacter ium were determined for batch cultures

with various concentrat ions of lactate and acetate, respect ively (Table 1).

The specif ic growth rate was calculated from the fornmla, /~ = (1 /x ) (dx / dt), and the doubling t ime from the formula, ta = ln2//x.

The results show that the doubling t ime of Methanobacterium is

always very long, about 70 hr. In both organisms the doubl ing time in-

creases at l imiting substrate concentrat ions.

Continuous Cultures. In a cont inuous culture the dilution rate (D) was

never a l lowed to exceed the critical dilution rate (De), because this would

result in gradual wash out of all the microorganisms. The rate of exchange

Table 1 Specific Growth Rates (It) and Doubling Times (td) o f D. desulfuricans and

Methanobacterium sp. at Various Substrate Concentrations for Batch Cultures a

Substrate Substrate added used la t d

(mg/ml) (mg/ml) (hr- 1 ) (hr)

D. desul furicans ~

Methanobacterium sp. c

6 6 0.26 2.7

3 3 0.14 5.0

1 1 0.04 17.5

0.1 0.1 0.034 20.3

0.05 0.05 0.015 46.2

0.5 0.43 0.011 65.0

0. I 0.1 0.011 65.0

0.025 0.025 0.008 86.5

aCultures were grown at 30~ The optical density of the cultures was continu- ously recorded in a recording biophotometer for 2 and 14 days, respectively.

bLactate added as substrate CAcetate added as substrate.

Page 5: microbial ecology

64 Th. E. Cappenberg

in the growth vessel is the flow rate in volume units per hour (f) against the working volume of the vessel ( V ) : f / V = D. During steady state conditions the concentration of microorganisms is constant, in other words D = /z. Continuous cultures were performed under conditions corresponding to those in the batch cultures with substrate concentrations which were shown to be growth-limiting (Table 1). The practicable values found were a dilution rate of 0.013 hr -1 with a substrate concentration in the reservoir (S~) of 0.1 mg/ml lactate for Desulfovibrio, and a dilution rate of 0.01 hr- : and a substrate concentration of 0.1 mg/ml acetate in experiments for Methanobacterium .

In order to determine optimal values of pH and redox potential for growth, the bacteria were cultured under varying pH or redox potential values (Table 2). The results indicate pH values of 7.4 and 7.1, and of redox potentials of - 1 4 0 mV and - 3 8 0 mV, respectively, for optimal growth of Desulfovibrio and Methanobacter ium. Consequently, in all con- tinuous culture experiments these values of pH and redox potential were maintained.

Maximum specific growth rates (/-/,max) and saturation constants (K~) for lactate- and acetate-limited growth, respectively, of Desulfovibrio and Methanobacterium were estimated graphically as double reciprocal plots of specific growth rate against substrate concentration. The results obtained are given in Fig. I.

The extrapolated lines indicate a /,/,max of 0.36 hr -1 and a K.~ for lactate-limited growth of 4.23 mg/liter for Desulfovibrio, and a /Zma• of 0.011 hr -1 and a K~ for acetate-limited growth of 10.2 rag/liter for Methanobacteriurn .

Besides the fall in growth rate with decreasing substrate concentra- tion, the yield of organisms also decreased. To explain such a relation between yield and growth rate, Pirt [10] proposed the relationship

I I m Y Y(; /x

where Y is the observed growth yield, Ya is the " t r u e " growth yield, i.e., the yield that would be obtained in the absence of a maintenance require- ment, and m is the maintenance coefficient (g substrate/g dry wt hr).

Plotting observed values of I/Y against 1//x (for specific growth rates in the range of 0.01 to 0.005 hr -1) for Methanobacterium sp. , a straight line and values of 0.63 g/g and 0.54 g/g hr for Ya and m, respectively, were obtained. Calculated values for Ya and m for D.desul furicans were 0.34 g/g and 0.53 g/g hr, respectively (Fig. 2). The values obtained for the mainte-

Page 6: microbial ecology

Tab

le

2 E

ffec

t o

f V

aryi

ng t

he p

H o

r th

e R

edo

x P

oten

tial

(E

h in

m V

) V

alue

s on

the

Dry

Wei

ght

(mg/

lite

r) V

alue

s b

y C

onti

nuou

s C

ulti

vati

on o

f D

. d

esu

lfu

rica

ns

(D=

0.

O13

hr-

1,

Sr

= 0.

I m

g/m

l L

acta

te)

and

Met

han

ob

acte

riu

m

sp.

(D =

0.0

1 hr

-1,

Sr=

0.1

m

g/m

l A

ceta

te)

D.

desu

lfu~

cans

M

etha

noba

cter

ium

sp.

E

h

D.

desu

lfur

ican

s M

etha

noba

cter

ium

sp.

p

H

(dry

w

t m

g/l

iter

) (d

ry

wt

rag

/lit

er)

(mV

) (d

ry w

t m

g/l

iter

) (d

ry

wt

mg

/lit

er)

6.8

- 3

2

7.0

38

68

7.1

- 78

7.2

50

56

7.4

8

2

28

7.5

70

-

7.8

22

-

0 26

-

-50

6

3

-

- 1

00

76

-

- 1

40

8

4

-

-1

80

6

5

-

-20

0

41

35

-25

0

- 57

-30

0

- 68

-35

0

- 76

-38

0

- 8

2

-40

0

- 78

e~

=__

Page 7: microbial ecology

66 Th. E. Cappenberg

' I

--0.4 1

K m

1 9 ! A

5

3

/ ' I / 1 1 ' I i I -0 .2 0 0.2 0,4

1 mg/I Lactate

ta 200

100

I / ' I ! -0.1 0 0.1 0,2

1 1 mg/I Acetate K m

Fig. 1. Double reciprocal plots of specific growth rate (/,z, hr -1) of Desu!fovibrio desulfuricans (A) and Methanobacterium sp. (B) at different substrate concentra- tions (g, rag/liter). Data obtained in steady-state conditions, which were assumed to have been reached when there had been no significant change in cell number for a minimum of four volume changes.

2

1

0 I 5O

A

J

I I I | i 100 150 50 100 150

l/Specific Growth Rate (HR - 1 )

Fig, 2. Double reciprocal plots of yield (g bacterial mass/g substrate)of Methano- bacterium (A) and Desulfovibrio (B) at different specific growth rates (~, hr- ' ) . Intercepts at the ordinates are the reciprocal values of Yc (g/g), slopes of the lines are the values of m (g substrate/g dry wt hr).

Page 8: microbial ecology

Mixed Continuous Cultures of Bacteria 67

Table 3 Effect of Varying the Hydrogen Sulfide Potential Values ( p S 2 - ) on the

Dry Weight (rag~liter) Values by Continuous Cultivation of M e t h a n o b a c t e r i u m sp. (D = 0.01 hr-1, S r = 0.1 mg/mlAcetate)

Methanobacterium sp. Methanobacterium sp. pS 2 - (d ry wt mg] l i t e r ) pSZ- (dry wt mg / l i t e r )

15 95 12.5

14.5 92 12

14 96 11.5

13.5 95 " 1 1

13 91

59

28

17

14

( - -X~x~X- - 'L - -x~X~x~X X ~X~X----X~x~> . . ~ , ~ z ~ A z~ 14 2 ~,.----

_a ~ , / ' CH4 if

0 1 _z~--z~z~ 3

~: ~ o Acetate

0! t=0.01 HR - 1 D =0.01 HR - 1

10 ~0

~ E M

~ o 5 10 ~ _ . . . _ _ _ . . . i D

~ , ~ o

I 0 o. I

A I B 0 l J I i

0 1 2 3 4 Time (HR X 100)

Fig. 3. Populat ion levels o f MethanobacteHum (M) and Desulfovibrio (D), and concentra t ions of acetate, methane , and pS'-'- values in first-state (A) and second- stage (B) cont inuous cultures. The effect of di lut ion on the start of the second-stage cont inuous cultures was elirninated by mult iplying the numbers of bacteria by a factor of 2. In the first stage the working volume of the cont inuous cultures was 300 ml; in the second stage, 600 ml.

Page 9: microbial ecology

68 Th. E. Cappenberg

nance coefficient were in the same order of magnitude as those quoted by Pirt [10] for anaerobic growth of various bacterial species.

Previous investigations [4, 5] indicated that existence at different depth in the mud of the maximum numbers of sulfate-reducing and methane-producing bacteria may be due to sensitivity of the latter group of bacteria to hydrogen sulfide produced by the former group. To confirm this the sensitivity of Methanobacterium sp. to hydrogen sulfide was tested in a continuous culture experiment by altering the sulfide concentration in the growth vessel. Dry weight (rag/liter) and pS 2- values (the hydrogen sulfide potential measured in the growth vessel at pH 7.1) are given in Table 3. For calculations of the actual sulfide ion strength in the medium, see Cappen- berg [4] . No growth was observed at apS 2- value beyond 11 and no effect on the growth at pS z- values greater than 13 was detected.

Mixed Continuous Cultures. In order to examine the behavior of the two bacterial species together a number of steady-state mixed-population levels were established. The two first-stage fermentors were operated over a range of dilution rates (D < Dc = 0.36 hr -~ forDesulfovibrio andD < D,. = 0.011 hr -1 for Methanobacterium) to provide a source of bacteria for the second-stage fermentor. Soon after the start of the mixed continuous culture

8

o 4

E ~z,

10

g~ 5 E 0 0 E

�9 ~ ro

n

0

pS= X -X

CH4

Acetate I

\ o/ I 14

D =0.01 HR - 1 D =0.01 HR - 1 Sr =0.1 MG/ML S r =0.5 MG/ML

tO

M D .g.~

10 'c~

, r~

0 1 2 3 4 5 6 7 8 9 10 Time (HR X 100)

Fig.4. Effect of increasing the substrate concentrations (Sr) o n the population levels of Methanobacterium (M) and Desulfovibrio (D), and concentrations of acetale, methane, and pS 2- values in mixed continuous cultures.

Page 10: microbial ecology

Mixed Continuous Cultures of Bacteria 69

an adjustment in the population levels occurred until a new steady-state was reached, It was assumed that steady-states was reached when there had been four volume changes during which time bacterial and substrate concentra- tions did not change significantly. The results of concentrations of bacteria and substrates are shown in Fig. 3. The population of Methanobacterium strongly increased, whereas that of Desulfovibrio showed a relatively small increase. Moreover, the concentration of acetate decreased, whereas that of methane increased. These results confirm that acetate produced by Desul-

fovibrio is indeed used by Methanobacterium.

If the substrate concentration in the reservoir was increased to 0.5 mg/ml, an inhibitoy effect on the growth of Methanobacterium was ob- served (Fig. 4). Further, when the concentration of hydrogen sulfide pro- duced by D.desulfuricans reached a pS 2- value of 10.5, the Methano- bacterium organisms were washed out of the culture vessel. Consequently, the concentration of acetate increased and the production of methane came to a standstill,

Discuss ion

The main object of this investigation was to supplement the existing information on the ecological relationship between sulfate-reducing and methane-producing bacteria in the mud of Lake Vechten [4-6] . In this study continuous cultures of both bacterial species were used. An acetate- fermenting methane bacterium was chosen, since previous studies have shown that acetate is an important precursor of methanogenesis in mud and that about 75% of the mixed population of methanogenic bacteria in Lake Vechten are of the acetate-fermenting type. Field observations showed that maximum numbers of methanogenic bacteria were recorded at depths of 3 to 6 cm in the mud, where the redox potential values of - 2 5 0 to - 3 0 0 mV and the pS 2- values were high (13.4 to 14.0). On the other hand, the maximum numbers of sulfate-reducing bacteria were found at depths of 0 to 2 cm in the mud, concomitantly with redox potential values of - 1 0 0 to - 1 5 0 mV andpS z- values of 11.4 to 12.2 [4, 5]. It was suggested that this difference in localization may be due to sensitivity of methanogenic bacteria to hydrogen sulfide.

In a continuous culture experiment in which the sensitivity of Methanobacterium sp. to hydrogen sulfide was tested, more evidence was found for this hypothesis (Table 3). No growth was observed at a pS z- value of less than 11 (hydrogen sulfide concentration of about O. 1 raM) and no effect was observed at pS 'z- values greater than 13 (hydrogen sulfide conc. of about 0.001 raM). Furthermore, when the substrate concentration was increased, the sulfide concentration increased and the methane bacteria were washed out from the mixed continuous culture (Fig. 4). From these

Page 11: microbial ecology

70 Th. E. Cappenberg

results we may conclude that the absence of methanogenic bacteria at depths in the mud where sulfate-reducing bacteria are abundant is due to the production of hydrogen sulfide by the latter to which the former are sensi- tive. Furthermore, since metbanogenic bacteria do not grow at a redox potential higher than - 2 0 0 mV (Table 2) this is an additional factor responsible for the different localities.

As very high values of pS ~ were measured in deeper layers in the mud [4] , it is likely that the hydrogen suffide is used either for the reduction of ferric to ferrous ions or is precipitated as FeS. Acetate, produced by the sulfate reducers, may diffuse and may be subsequently used as a substrate by methane producers. From the results of the mixed continuous culture experiments (Fig. 3) of the two bacterial species the existence of a commensalism can be noted. On the start of the mixed continuous culture an adjustment in the population levels occurred until a new steady-state was reached in the second stage. Particularly, a large increase in the level of the Methanobacterium population was observed. The concentration of acetate decreased, whereas that of methane increased stoichiometrical ly. The interaction of D.desulfuricans and Methano- bacterium sp. is a true commensalism in that one organism, the acetate- fermenting Methanobacterium, benefits from the products (acetate) released by Desulfovibrio, which, in turn, is effected in no way by the presence of the former. Such a substrate relationship between the two groups in the sediments of Lake Vechten has already been suggested [6] , since labeled methane was detected when mud containing both methanogenic and sulfate-reducing bacteria was incubated with uniformly labeled lactate.

The ecological association of the two species is, however, compli- cated by the release by D. desulfuricans of hydrogen sulfide which inhibited the growth of the Methanobacterium (Fig. 4). If the substrate concentration in the reservoir (St) was increased to 0.5 mg/ml, the concentration of hydrogen sulfide reached a level of a pS" - value of 10.5, consequently growth of the methanogenic bacteria is inhibited, It has already been shown by selective inhibition and by enhancement of sulfate-reducing and methanogenic processes in mud [4, 5] that SO42- is the limiting factor for the abundance of sulfate-reducing bacteria. Addition of sulfate to mud samples caused an increase in the concentration of hydrogen sulfide and inhibition of methanogenesis.

Summarizing the ecological relationship between the two bacterial types in the lake mud, we find that hydrogen sulfide and acetate produced by sulfate reducers which are in the upper layers sets up two diffusion gradients-- those of H2S and acetate. Hydrogen sulfide would be partly or completely oxidized depending on the concentration of ferric ions in the mud. On the other hand, the shape of the acetate gradient would be

Page 12: microbial ecology

Mixed Continuous Cultures of Bacteria 71

influenced by the depth at which methane producers are present and would also determine the concentration of acetate-fermenting methanogenic bac- teria.

Last, quantifying the growth of the bacteria in the first-stage continu- ous cultures the yield corrected for maintenance of Methanobacterium sp. growing on acetate was high (Ya = 37.8 g/mole). Molar growth yields of D.desulfuricans growing on lactate recorded in this study are substantially higher (Y~; = 30.6 g/mole) than those reported for batch cultures by Senez [14]. Stouthamer and Bettenhausen [16] and Stouthamer [15] calculated a production of approximately 30 g bacterial mass/mole of ATP. This seems to indicate a production of about I mole of ATP per mole of acetate fermented by Methanobacterium and a somewhat higher ATP yield per mole of lactate fermented by Desulfovibrio.

Acknowledgments

The skillful technical assistance of Mr. H. Korthals is gratefully acknowledged. The author is indebted to Professor A.H. Stouthamer for his critical review of the manuscript.

References

1. Baker, K. 1968. Low cost continuous culture apparatus. Lab. Pra t t . 17: 817-824.

2. Barker, H.A. 1936. Studies upon the methane-producing bacteria. Arch. Mikrobiol . 7: 420-438.

3. Cappenberg, Th. E. 1972. Ecological observations on heterotrophic, methane oxidiz- ing and sulfate reducing bacteria in a pond. Hydrobiologia 40:471-485.

4. Cappenberg, Th. E. 1974. Interrelations between sulfate-reducing and methane- producing bacteria in bottom deposits of a fresh-water lake. I. Field observa- tions. Antonie van Leeuwenhoek 40: 285-295.

5. Cappenberg, Th. E. 1974. lnterrehttions between sulfate-reducing and inethane- producing bacteria in bottom deposits of a fresh-water lake. II. Inhibition exper- iments. Antonie van Leeuwenhoek 40: 297-306.

6. Cappenberg, Th. E. and Prins, R.A. 1974. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. IIl. Exper- iments with HC-labeled substrates. Antonie van Leeuwenhoek 40: 457-469.

7. Gemerden van, H. 1967. On the bacterial sulphur cycle of inland waters. Thesis,

Leiden.

8. Hungate, R.E. 1969. A roll tube inethod for cultivation of strict anaerobes. In:

Methods in Microbiology. J.R. Noris and D.W. Ribbons, editors. Vol. 3B, pp. 117-132. Acadelnic Press, New York.

9. Mylroie, R.L. and Hungate, R.E. 1954. Experiments on the methane bacteria in sludge. Can. J. Microbiol . l: 55-64.

10. Pirt, S.J. 1965. The maintenance energy of bacteria in glowing cultures. Proc. Royal

Soc. B 163: 224-231.

Page 13: microbial ecology

72 Th. E. Cappenberg

11. Postgate, J. 1965. Enrichment and isolation of sulfate-reducing bacteria. Zbl. Bakt. 1. Abtl., Supplementheft 1: 190-197.

12. Postgate, J. and Campbell, L.L. 1966. Classification of Desulfovibrio species, the nonsporulating sulphate-reducing bacteria. Bact. Rev. 30: 732-738.

13. Pretorius, W.A. 1972. The effect of formate on the growth of acetate utilizing methanogenic bacteria. Water Res. 6: 1213-1217.

14. Senez, J.C. 1962. Some considerations on the energetics of bacterial growth. Bact. Rev. 26: 95-107.

15. Stouthamer, A.H. 1973. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie van Leeuwenhoek 39: 545-565.

16. Stouthamer, A.H. and Bettenhaussen, C. 1973. Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. A reevalua- tion of the method for the determination of ATP production by measuring molar growth yields. Biochim. Biophys. Acta 301: 53-70.

17. Tempest, D.W. 1970. The cultivation of micro-organisms. 1. Theory of the chemostat. In: Methods in Microbiology. J.R. Noris and D.W. Ribbons, editors. Vol. 2, pp. 259-276. Academic Press, New York.

18. Veldkamp, H. and Jannasch, H.W. 1972. Mixed culture studies with the chemostat. J. Appl. Chem. Biotechnol. 22" 105- 123.

19. Wolfe, R.S. 1971. Microbial formation of methane. Advan. Microbial Physiol. 6: 107- 146.

20. Zeikus, J,G. and Wolfe, R.S. 1972. Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J. Bacteriol. 109: 707-713.