MI SoftwareHigh Performance Image Analysisand Publication Tools

Innovation with IntegrityPreclinical Imaging

Molecular Imaging (MI) Software provides high performanceimage analysis for a wide range of molecular imagingapplications. Featuring a comprehensive set of tools forquantitation, image display, databasing, and reporting,MI Software improves the quality and ease of image analysis forin vivo and ex vivo samples such as small animal tissue, plates andmore. An intuitive navigational structure featuring workflow driventool palettes further streamlines the optimization and analysis ofimages.

� Supplied with all Bruker In-Vivo imaging systems; also compatible withTIFF or JPEG image file formats

� Advanced image display control including image filters, pseudocolors,feature masking, histogram adjustment and image overlay capabilities.

� Comprehensive image analysis tools for quantitation of molecular imagingdata in features of interest such as tumors, cells, and more.

� User-friendly navigation features a palette-driven interface for convenientaccess to toolbars and commands

� Windows 2000/XP 7 and Macintosh OSX single user, multi-user andregulatory versions available

Comprehensive region of interest (ROI) analysis capabilityprovides intensity, size and position, and comparative

data for user-defined features of interest

Multiplex tools for image overlay and easy analysisof up to four images simultaneously

MI software provides users pushbutton accessto its many tools and applications throughthe Navigation bar:

Image - Tools for optimizing image presentationManual ROIs - Applications for drawing regionsof interest and quantify imaging dataAutomatic ROIs - Functions for automaticallyfinding and quantifying regions of interestGrid ROIs - Functions for quantifying plateand array dataLanes - Tools for quantifying gels and blotsAnnotation - Tools for presenting imaging datain notebooks and publicationsDatabase - Functions for searching and storingimaging datasets

Molecular Imaging Software

Region of Interest (ROI) PanelsDefine, measure, and count specific regions of interest such astumors, bacterial colonies, plates, cells in culture, gels and more

� Select ROIs manually, utilize themagic wand tool, oruse an automated detection algorithm (edge detection,threshold, density slice, or peak finder) to define specificimage features

� Selectively display a wide range of quantitative values for eachROI, including intensity, geometry, and positional values

� Produce comparative values versus a reference ROI for rapiddetermination of relative intensity, size, etc.

� Set two or more ROIs asmass standards from which massvalues for experimental ROIs can be derived

� Select proper fit for data points within a selected mass curve

MI Software’s manual and automated ROI definition tools allow fastand easy identification of a wide variety of features of interest

Manual-ROIs Auto-ROIs Grid-ROIs

Image PanelGain precise control of image appearanceon screen and in print

� Image display provides access to image histogram,brightness/contrast/gamma control, filters,pseudocolors, and more

� Feature masking and image overlay allow features of interestto be selected and merged with other image files

� Rotate, flip, and crop tools allow proper orientation and displayof features of interest

� Image math and image correction provide mathematical toolsthat can be applied to a single image or pair of images

Image

MI Software sets the standard for high-performanceimage analysis and simplicity of operation. The key?An innovative, user-friendly navigation system thatuses a collection of workflow-driven tool palettes.MI Software provides fast access to specific tool setsfor viewing and analysis of in vivo images, plates,bacterial colonies, westerns, gels and more.

Better results, faster results, easier results—they’reall within your grasp with Bruker’s MI Software suiteof products.

Powerful Tools, Just a Click Away

Sophisticated software algorithms remove autofluorescence forimproved signal to noise and detection sensitivity. This allows forimproved use of multiple fluorescent agents (i.e. multipexing) in asingle sample as well as the removal of non-specific fluorescence.Custom spectral models for various elements can be generated viaa graphical user interface that also facilitates simple managementof spectral models.

Pushbutton launch into the complementary Molecular ImagingSoftware allows for optional analysis of spectral elements and acontinuous workflow. Multimodal data sets including multispectral,X-ray, luminescent, and/or radioisotopic detections are displayedin registration and with image presentation features provided.Multispectral Software is designed for use with the In-VivoMS FX PRO and Xtreme systems.

MI Software Regulatory EditionSupports your compliance with U.S. FDA Code ofFederal Regulations, 21 CFR, Part 11

� Security features include user identification, data management,change logging, digital signatures, and more to assist with U.S.FDA 21 CFR, Part 11 compliance

� Seamlessly stores images, image meta-data, and analysisresults within MI Software and Bruker optical/X-rayimaging systems

� Intuitive controlsmake it easy to submit, review, and approveelectronic records

� Audit trail toolsmaintain and preserve a record of all raw dataand create a complete audit trail of any changes made to the filespecifying user ID, time, and date

� Cross-platform supports eitherMacintosh orWindows usersand is fully upgradeable from previous versions

Special Featuresfor Advanced Applications

Multispectral SoftwareSupports spectral modeling and unmixing of fluorescentsample elements in vivo

Bone Density SoftwarePerform bone density analysis of small animals in vivo

The Bone Density Software Module is designed for a wide range ofapplications ranging from the study of bone diseases, such asarthritis and osteoporosis, to the efficacy of drugs and othertreatment options that may affect bone mineral and other densities.Sophisticated numerical analysis using a cylindrical model providesmeasurements not only of the density of the bone, but also of thebone marrow, the size of the bone and the thickness of the bonewall, all important parameters for tracking and monitoring diseasedevelopment. Designed for use with the In-Vivo MS FX PRO,FX PRO, DXS PRO and Xtreme systems.

MARS Rotation SoftwareSupports 360° imaging of your subject so that you nevermiss an optical signal due to animal position

MARS rotation software, for use with theMARSMultimodalAnimal Rotation System, enables automatic co-registration andvisualization of multimodal and multispectral data sets from allacquired angles. Increase sensitivity by quantifying the perfectimage or simply export an entire rotation movie.

� Images can be cropped or zoomedto highlight specific features

� Drag & drop quantitative valuesfrom the analysis window; or typelane labels, figure legends, andother text information using avariety of colors and fonts

� Intensity profile, mass curve,and other project elements canbe added to the annotation canvasas desired

� An intensity scale can be displayed to provide a quantitativemap of pseudocolor intensity

Database Panel—Project DatabaseUtilizes image and file information to identifyspecific projects stored in the database

� Search terms include 19 different options, such as captureconditions, standards used, capture time and date,user-defined fields and more

� Image thumbnails and key file attributes for each projectmeeting the search criteria are displayed; one or moreprojects can be launched directly from the databaseresults window

Annotation

Database

Annotation PanelProvides a canvas for formatting images, text,graphics, and data for publication purposes

Lanes PanelProvides comprehensive analysis of nucleicacids and proteins in gels and blots

� Auto-lane definition and auto-band findingmake analysis ofelectrophoresis gels fast and easy

� Multiple lane set capability allows rapid analysis of high-throughput gel formats

� An intensity profile window allows viewing and editing of bandboundaries and background definition for each lane

� Gaussian deconvolution improves analysis accuracy ofsaturated and overlapping bands

� Over 25 nucleic acid and proteinmolecular weight and/or massstandards are conveniently pre-defined for use in quantitatingunknown bands. New standards can be added and saved quicklyand easily

Lanes

© Bruker Corporation Printed in U.S.A. December 2012

Find out moreFor more information or to place an order,

call 1-978-667-9580, Option 4.

Or visit us online at

www.bruker.com

Imaging System CompatibilityMolecular Imaging Software contains integrated interfaces for In-Vivo cameras. In addition, MI Software is

compatible with TIFF and JPEG image files captured using other imaging devices. MI Software’s n-bit file format

capability and floating point data utilize all image file data for quantitative analysis.

System RequirementsOperating Systems Windows XP Professional (sp 3 or greater)(Windows) Windows 7 Professional

Operating Systems (MAC) OS X (10.5 or higher) Not compatible with In-Vivo Xtreme

Memory 2 GB (minimum) 8 GB recommended for In-Vivo Xtreme

Monitor 1280 x 1024 minimum

Required Ports USB, Built-in Gigabit Ethernet

Available Software ConfigurationsMI Software is supplied as copy-protected single or multi-user packages forWindows and MAC

operating systems. MI Software Regulatory Edition is available in network versions only.

Worldwide Service, Training and Technical SupportAt Bruker, we want your research programs to succeed, so we are here to support you with a comprehensive suite

of service, training and technical support programs that are second to none:

— A comprehensive warranty, backed by an expert service team

—A choice of service packages from basic to premium and preventive maintenance

—A range of technical support options including phone support and remote access support

— Application support by our team of PhD scientists

— Problem solving assistance by our imaging experts and highly responsive world-wide support team

—Training programs for users at all skill levels.

About Bruker CorporationBruker Corporation, a $1.5 billion public company with 6,200 employees worldwide, is a global technology and

market leader in magnetic resonance imaging (MRI) magnetic particle imaging (MPI) and X-ray micro computer

tomography (micro CT). With the addition of high resolution Optical, X-ray, PET, SPECT and CT systems, the Bruker

BioSpin imaging portfolio becomes unparalleled in the market, offering you the most diversified choice of imaging

modalities for small animal preclinical research.

Bruker BioSpin Corporation • 4 Research Drive • Woodbridge, CT 06525