Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF...
Transcript of Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF...
![Page 1: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/1.jpg)
Membrane Structure and Function
![Page 2: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/2.jpg)
Fig. 7-3
Phospholipid
bilayer
Hydrophobic regionsof protein
Hydrophilicregions of protein
![Page 3: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/3.jpg)
Negative Feedback in a cell membrane
• The steroid cholesterol has different effects on
membrane fluidity at different temperatures
• At warm temperatures (such as 37°C),
cholesterol restrains movement of
phospholipids
• At cool temperatures, it maintains fluidity by
preventing tight packing
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 4: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/4.jpg)
Fig. 7-5c
Cholesterol
(c) Cholesterol within the animal cell membrane
![Page 5: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/5.jpg)
Fig. 7-7
Fibers ofextracellularmatrix (ECM)
Glyco-protein
Microfilamentsof cytoskeleton
Cholesterol
Peripheralproteins
Integralprotein
CYTOPLASMIC SIDEOF MEMBRANE
GlycolipidEXTRACELLULARSIDE OFMEMBRANE
Carbohydrate
Structure of Cell Membrane
![Page 6: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/6.jpg)
Membrane Proteins and Their Functions
• Peripheral proteins are bound to the surface
of the membrane
• Integral proteins penetrate the hydrophobic
core
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 7: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/7.jpg)
Selective permeability of cell membrane
• A cell must exchange materials with its
surroundings, a process controlled by the
plasma membrane
• Plasma membranes are selectively permeable,
regulating the cell’s molecular traffic
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 8: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/8.jpg)
The Permeability of the Lipid Bilayer
• Hydrophobic (nonpolar) molecules, such as
hydrocarbons, can dissolve in the lipid bilayer
and pass through the membrane rapidly
• Polar molecules, such as sugars and water,
do not cross the membrane on their own
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 9: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/9.jpg)
Transport Proteins
• Transport proteins allow passage of
hydrophilic substances across the membrane
• Some transport proteins, called channel
proteins, have a hydrophilic channel that
certain molecules or ions can use as a tunnel
• Channel proteins called aquaporins facilitate
the passage of water
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 10: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/10.jpg)
• Other transport proteins, called carrier
proteins, bind to molecules and change shape
to shuttle them across the membrane
• A transport protein is specific
for the substance it moves
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 11: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/11.jpg)
Passive transport is diffusion of a substance across a membrane with no energy investment
• Diffusion is the tendency for molecules to
spread out evenly into the available space
• The diffusion of a substance across a biological
membrane is passive transport because it
requires no energy from the cell to make it
happen
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 12: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/12.jpg)
Fig. 7-11Molecules of dye Membrane (cross section)
WATER
Net diffusion Net diffusion Equilibrium
(a) Diffusion of one solute
Net diffusion
Net diffusion
Net diffusion
Net diffusion
Equilibrium
Equilibrium
(b) Diffusion of two solutes
![Page 13: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/13.jpg)
Effects of Osmosis on Water Balance
• Osmosis is the diffusion of water across a
selectively permeable membrane
• Water diffuses across a membrane from the
region of lower solute concentration to the
region of higher solute concentration
• Ex. Kidney function
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 14: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/14.jpg)
Lower
concentrationof solute (sugar)
Fig. 7-12
H2O
Higher
concentrationof sugar
Selectivelypermeable
membrane
Same concentration
of sugar
Osmosis
![Page 15: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/15.jpg)
Water Balance of Cells Without Walls
• Tonicity is the ability of a solution to cause a
cell to gain or lose water
• Isotonic solution: Solute concentration is the
same as that inside the cell; no net water
movement across the plasma membrane
• Hypertonic solution: Solute concentration is
greater than that inside the cell; cell loses
water
• Hypotonic solution: Solute concentration is
less than that inside the cell; cell gains waterCopyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 16: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/16.jpg)
Fig. 7-13
Hypotonic solution
(a) Animal
cell
(b) Plant
cell
H2O
Lysed
H2O
Turgid (normal)
H2O
H2O
H2O
H2O
Normal
Isotonic solution
Flaccid
H2O
H2O
Shriveled
Plasmolyzed
Hypertonic solution
![Page 17: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/17.jpg)
• Hypertonic or hypotonic environments create
osmotic problems for organisms
• Osmoregulation, the control of water balance,
is a necessary adaptation for life in such
environments
• The protist Paramecium, which is hypertonic to
its pond water environment, has a contractile
vacuole that acts as a pump
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 18: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/18.jpg)
Fig. 7-14
Filling vacuole 50 µm
(a) A contractile vacuole fills with fluid that enters froma system of canals radiating throughout the cytoplasm.
Contracting vacuole
(b) When full, the vacuole and canals contract, expelling
fluid from the cell.
![Page 19: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/19.jpg)
Facilitated Diffusion: Passive Transport Aided by Proteins
• In facilitated diffusion, transport proteins
speed the passive movement of molecules
across the plasma membrane
• Channel proteins provide corridors that allow a
specific molecule or ion to cross the membrane
• Channel proteins include
– Aquaporins, for facilitated diffusion of water
– Ion channels that open or close in response
to a stimulus (gated channels)
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 20: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/20.jpg)
Fig. 7-15
EXTRACELLULAR FLUID
Channel protein
(a) A channel protein
Solute CYTOPLASM
Solute Carrier protein
(b) A carrier protein
![Page 21: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/21.jpg)
• Some diseases are caused by malfunctions in
specific transport systems, for example the
kidney disease cystinuria
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 22: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/22.jpg)
Active transport uses energy to move solutes against their gradients
• Facilitated diffusion is still passive because the
solute moves down its concentration gradient
• Some transport proteins, however, can move
solutes against their concentration gradients
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 23: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/23.jpg)
The Need for Energy in Active Transport
• Active transport moves substances against
their concentration gradient
• Active transport requires energy, usually in the
form of ATP
• Active transport is performed by specific
proteins embedded in the membranes
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 24: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/24.jpg)
• Active transport allows cells to maintain
concentration gradients that differ from their
surroundings
• The sodium-potassium pump is one type of
active transport system
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 25: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/25.jpg)
2
EXTRACELLULAR
FLUID[Na+] high
[K+] low
[Na+] low
[K+] high
Na+
Na+
Na+
Na+
Na+
Na+
CYTOPLASM
ATP
ADP
P
Na+
Na+
Na+
P
3
6 5 4
PP
1
Fig. 7-16-7
![Page 26: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/26.jpg)
Fig. 7-17Passive transport
Diffusion Facilitated diffusion
Active transport
ATP
![Page 27: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/27.jpg)
How Ion Pumps Maintain Membrane Potential
• Membrane potential is the voltage difference
across a membrane
• Voltage is created by differences in the
distribution of positive and negative ions
• Ex. Nerve signal transmission
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 28: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/28.jpg)
Bulk transport across the plasma membrane occurs by exocytosis and endocytosis
• Small molecules and water enter or leave the
cell through the lipid bilayer or by transport
proteins
• Large molecules, such as polysaccharides and
proteins, cross the membrane in bulk via
vesicles
• Bulk transport requires energy
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 29: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/29.jpg)
Exocytosis
• In exocytosis, transport vesicles migrate to the
membrane, fuse with it, and release their
contents
• Many secretory cells use exocytosis to export
their products
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 30: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/30.jpg)
Endocytosis
• In endocytosis, the cell takes in macromolecules
by forming vesicles from the plasma membrane
• There are three types of endocytosis:
– Phagocytosis (“cellular eating”)
– Pinocytosis (“cellular drinking”)
– Receptor-mediated endocytosis
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 31: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/31.jpg)
• In phagocytosis a cell engulfs a particle in a
vacuole
• The vacuole fuses with a lysosome to digest
the particle
• In pinocytosis, molecules are taken up when
extracellular fluid is “gulped” into tiny vesicles
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 32: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/32.jpg)
Fig. 7-20a
PHAGOCYTOSIS
CYTOPLASM EXTRACELLULAR
FLUID Pseudopodium
“Food” or
other particle
Foodvacuole Food vacuole
Bacterium
An amoeba engulfing a bacterium
via phagocytosis (TEM)
Pseudopodium
of amoeba
1 µm
![Page 33: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/33.jpg)
Fig. 7-20b
PINOCYTOSIS
Plasmamembrane
Vesicle
0.5 µm
Pinocytosis vesicles
forming (arrows) in
a cell lining a small
blood vessel (TEM)
![Page 34: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/34.jpg)
• In receptor-mediated endocytosis, binding of
ligands to receptors triggers vesicle formation
• A ligand is any molecule that binds specifically
to a receptor site of another molecule
• Ex: familial hypercholesterolmia
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
![Page 35: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/35.jpg)
Fig. 7-20cRECEPTOR-MEDIATED ENDOCYTOSIS
Receptor
Coat protein
Coatedpit
Ligand
Coatprotein
Plasmamembrane
0.25 µm
Coatedvesicle
A coated pitand a coatedvesicle formedduringreceptor-mediatedendocytosis(TEMs)
![Page 36: Membrane Structure and Function - Stagg High School ... · Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate Structure of Cell Membrane. Membrane Proteins and Their Functions](https://reader031.fdocuments.us/reader031/viewer/2022030702/5aede5977f8b9a572b8bf4cd/html5/thumbnails/36.jpg)
Fig. 7-UN3
Environment:
0.01 M sucrose
0.01 M glucose
0.01 M fructose
“Cell”
0.03 M sucrose
0.02 M glucose