Medical School Histology Basics Epithelium and junctions · Epithelium - function Epithelial...
Transcript of Medical School Histology Basics Epithelium and junctions · Epithelium - function Epithelial...
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Medical School Histology Basics Epithelium and junctions
VIBS 289 lab
Larry Johnson Texas A&M University
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Epithelium - function • Epithelium forms the outer protective surface of the
body and all the glands. It lines the walls of most closed cavities of the body and all passages leading to the exterior, and it lines blood and lymphatic vessels.
skin gland kidney cavities bladder vessels
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Epithelium - function
Epithelial tissues participate in the metabolism of the body through: i) the absorption of substances from the exterior, e.g., from the lumen of the intestines; and ii) the elimination of other substances to the outside. All substances, including gases, normally received and given off from the body must pass through epithelia. For the performance of secretory functions, the epithelial tissues form specialized structures called glands.
Intestine Sweat gland
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Origin and Distribution of Epithelium
Ectoderm - epidermis of skin and epithelium of cornea together covers the entire surface of the body; sebaceous and mammary glands
Endoderm - alimentary tract,
liver, pancreas, gastric
glands, intestinal glands • Endocrine glands - lose
connection with surface
Mesoderm • Endothelium - lining of blood vessels
• Mesothelium - lining serous cavities
ECTODERM
ENDODERM
MESODERM
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CLASSIFICATION OF EPITHELIA
NUMBER OF LAYERS – SIMPLE - ONE LAYER
– STRATIFIED - MORE THAN ONE LAYER
SHAPE OF SUPERFICIAL CELLS – SQUAMOUS - FLAT
– CUBOIDAL - CUBE
– COLUMNAR – COLUMN
OTHER
– PSEUDOSTRATIFIED - NOT TRULY STRATIFIED
– TRANSITIONAL - URINARY TRACT
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Slide 109: Skin, hand, monkey Simple squamous endothelial cells lining
blood lymph vessels – Stratified squamous epithelium of skin – Stratified cuboidal of sweat ducts
Stratified cuboidal
dermis
dermis
epidermis Sweat gland
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Rat intestine Smooth muscle
Small intestinal villi
Simple columnar epithelium
Goblet cells
148
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EM 3: region of basal lamina and
connective tissue beneath intestinal
epithelial absorptive cells
1. Plasma membrane
2. Basal lamina
3. Connective tissue Goblet cell
Intestinal absorptive cell nuclei
Brush border
32409 EM 3
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EM 3: Intestine (Basal part of cell)
Basal lamina
Intestinal
absorptive cells Connective
tissue
148
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Slide 258: Kidney (PAS) Basement membrane of epithelium
Simple squamous epithelium
Simple cuboidal in medulla and cortex
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Ileum 148
Simple columnar epithelium
Intestinal absorptive cells and goblet cells
Goblet cells
Brush border
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Ileum, monkey (PAS)
249
Basement membrane of epithelium Simple columnar epithelium
Goblet cells
Brush border
Connective tissue
Cell nuclei
Basement membrane, brush border and goblet cells are PAS positive for sugars
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EM 10f
The basal lamina of epithelial cells usually is thin (EM 10f); however, under pathological conditions, it is thickened as in the lymphatic vessel and seminiferous epithelium (Sertoli cell) of an aged-rat testis (EM 8h).
EM 8h
basal lamina
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SPECIALIZATION OF EPITHELIA
MAINTAIN EXTENSIVE
CONTACTS AMONG CELLS
STRUCTURALLY AND FUNCTIONALLY POLARIZED
JUNCTIONS ZONULA OCCLUDENS - TIGHT JUNCTION (BELT)
ZONULA ADHERENS –
ADHERING BELT
DESMOSOME (MACULA
ADHERENS) - SPOT ATTACHMENT
GAP JUNCTIONS - COMMUNICATION
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Terminal bars
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148 250
250
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Terminal bars
Terminal bars are part of a belt (zonula) around the cells.
belt
Intestinal absorptive cells
Goblet cells
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EM 4: Intestine (Apical)
Tight junction,
zonula adherens,
terminal web
148
Goblet cells
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EM 2: Liver
– Gap junction,
– desmosome,
tight junction
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EM 2a: Liver- Gap junctions
EM 2
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133 133
250
Simple squamous epithelium cells viewed on their sides
vein
Lumen of lymphatic vessel
valve
valve capillary
Simple squamous epithelium cells viewed “face on”
venule
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Simple squamous epithelium
Lumen of lymphatic vessels
arteriole
valve 109
19680 testis
196
valve
vein
capillary
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EM 10A showing capillary endothelial cells
109 Simple squamous epithelium : cells viewed on the side
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Stratified squamous epithelium 109
Keratinized dead flattered cells at surface
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Slide 109: Skin, hand, monkey prickle cell" layer (desmosomes)
dermis
epidermis
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Slide 109 : Skin, hand, monkey
Stratified cuboidal is only found in sweat ducts of skin (Slide 109).
Fat cells
epidermis dermis
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HISTO052 tongue Vagina (Slide 178)
non-keratinized stratified squamous epithelium
Nuclei present in flat cells at surface
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Slide 133: Trachea, monkey
– Simple squamous lining the air spaces of lungs
– Pseudostratified epithelium lining tracheal lumen
– Ciliated epithelium of trachea, goblet cells, thick basement membrane
Lung air space
432
133
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tracheal lumen (Slide 133),
transition of the luminal epithelium from stratified squamous of vocal cords to pseudostratified, ciliated epithelium
human larynx, Slide 429
stratified squamous
Note thickened basement membrane typical of this epithelium
pseudostratified, ciliated epithelium called respiratory epithelium
Plasma cells 133
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Transitional epithelium urinary bladder (Slide 160) ureter (Slide 262)
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Slide 160 Image of the Urinary bladder
Transitional epithelium
Mesothelium
Lumen of bladder
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HISTO052 (tongue) to observe mucus and serous glands
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In summary
All substances, including gases, normally received and given off from the body must pass through epithelia.
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Which junction - description pair match? a. Zonula occludes – tight junction around the cell b. Zonula adherens – adhering junction around the cell c. Hemidesmosome – spot attachment of cells to the CT below d. a & b e. a, b, & c
Which item(s) is/are characteristic of epithelia? a. secretory cells of glands b. covers organs c. line urinary tract d. a & b e. a, b, & c
Questions on Epithelia
Which embryonic origin - distribution of epithelium do not match? a. endoderm - endothelium b. endoderm - alimentary tract c. mesoderm - mesothelium d. ectoderm - mammary gland e. ectoderm - epidermis
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• Bruce Alberts, et al. 1983. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY. • Bruce Alberts, et al. 1994. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY.
• William J. Banks, 1981. Applied Veterinary Histology. Williams and Wilkins, Los Angeles, CA.
• Hans Elias, et al. 1978. Histology and Human Microanatomy. John Wiley and Sons, New York, NY.
• Don W. Fawcett. 1986. Bloom and Fawcett. A textbook of histology. W. B. Saunders Company, Philadelphia, PA. • Don W. Fawcett. 1994. Bloom and Fawcett. A textbook of histology. Chapman and Hall, New York, NY.
• Arthur W. Ham and David H. Cormack. 1979. Histology. J. S. Lippincott Company, Philadelphia, PA.
• Luis C. Junqueira, et al. 1983. Basic Histology. Lange Medical Publications, Los Altos, CA. • L. Carlos Junqueira, et al. 1995. Basic Histology. Appleton and Lange, Norwalk, CT.
• L.L. Langley, et al. 1974. Dynamic Anatomy and Physiology. McGraw-Hill Book Company, New York, NY.
• W.W. Tuttle and Byron A. Schottelius. 1969. Textbook of Physiology. The C. V. Mosby Company, St. Louis, MO.
• Leon Weiss. 1977. Histology Cell and Tissue Biology. Elsevier Biomedical, New York, NY. • Leon Weiss and Roy O. Greep. 1977. Histology. McGraw-Hill Book Company, New York, NY.
• Nature (http://www.nature.com), Vol. 414:88,2001. • Arthur C. Guyton,1971.Textbook of Medical Physiology W.B. Saunders company, Philadelphia, PA • WW Tuttle and BA Schottelius 1969 Textbook of Physiology C.V. Mosby Co.
• A.L. Mescher 2013 Junqueira’s Basis Histology text and atlas, 13th ed. McGraw
Many illustrations in these VIBS Histology YouTube videos were modified from the following books and sources: Many thanks to original sources!
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Ranch just south of Fort Stockton, TX
On road from Fort Stockton to Big Bend National Park, TX
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The end of