Measuring Amount Bacteria by MPN Method
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Transcript of Measuring Amount Bacteria by MPN Method
Measuring Amount of Bacteria by
Index of The Most Probable Number
Menu1 •Principle
2 •Equipments
3 •Material
4 •Procedure
5 •Work Chart
6 •Conclusion
Principle
– In determining amount of bacteria, solid or liquid sample is for diluting in 10-1 until 10-3 . Inoculation with TSB as media, and then will inkubating in 370 C for 24 hour. In every diluting also have to observeted carefully the muddy of solution, and check the result we get dapand on the The Most Logical Number Table
EquipmentsSterill of Erlenmeyer
Ulir tube
Serological pipette 1 ml
Serological pipette 5 ml
Serological pipette 10 ml
Colony counter
Spirtus burner
Plastic wash bottle
Bulb
• The Material which need:1. BPW (Bacto Peptone Water)2. LF3. Sample Solid4. TSB media (Tryptic Soy Broth)5. Rinse alcohol
Materials
Add 9 ml BPW
solution into tube I, II, III,
IV
Pipette 1 ml sample, get into tube I,
homogenize stick label10-
1 Pipette 1 ml sample from
tube I, get into three of
every ulir tube
Pipette 1 ml sample from
tube I, get into tube II.
Homogenize (stick label 10-
2)Pipette 1 ml
sample from tube II, get into three of
every ulir tube
Pipette 1 ml sample from tube II, get
into tube III. Homogenize
(stick label 10-
3)Do an
effectivity test
Do an sterillity test
Incubate all of ulir tube in
inkubator 370
Procedure
Work Chart
Conclusion
THANKS FOR YOUR KIND ATTENTION
: DD
Presented by:
Husnun Hanifah
Isbir Farhan
Mochammad Fajar
Nadia Nahla K
Nuruzzahra A