MCP Newsletter

Department of Molecular &

Cellular Physiology

MCP Newsletter

September 2021

Inside this issue:

New Students 2

Publications 3-4

Grants 5

Highlights 6-8

Seminar Speaker 9

Congratulations to Our 2021 MCP Graduate

Welcome New Technicians, Research Associates and Post-Doctoral Fellows

New Technicians

Isaiah Crosbourne– Barroso Lab

Austin Lown– Goo/Paul Lab

Rachel Mannino– Wang Lab

Kailie Matteson– Barroso Lab

Sean Walis– Fredman Lab

Research Associate

Yidi Wu– Tang Lab

Yinna Wang– Tang Lab

Post Doctoral Fellows

Ed Wilson Cavalcante Oliveira

Santos– Wang Lab

Raymond Abini-Agbomson Raymond Abini-Agbomson, MS thesis title: “The complex role of rab4 in endo-somal-related processes in breast cancer cells”; Ray has been accepted into AMC Medical School program

Please Join us in Welcoming New Students of MCP Dr. Singer, MCP Chair

“I am delighted to welcome our new graduate students onto

the MCP team. As trainee’s in our program they are key ele-

ments of both our educational and research missions. Just as

we have in the past, I ask everyone on the MCP team to open

your doors and minds to the benefit of our new students –

“paying forward” the educational, research and mentoring

experiences that have shaped each of us.”

Dr. Ginnan, Graduates Studies Director

“We are excited to welcome two MS students and three PhD

students to MCP this year. Some are originally from the Capital

region and attended a local college or university while others are

international students who are continuing their training here in

Albany. We are looking forward to seeing them grow and what

they will achieve in MCP.”

MCP Newsletter 2

Please Join us in Welcoming New Students of MCP

Kurrim Gilliard Hi! My name is Kurrim (Kuh-Rim), and I’m from a small town in Sullivan County NY. I

moved to Albany to get my bachelor’s in biology from SUNY Albany where I graduated in

2019. After spending two years as a lab tech in the Jourd’heuil lab, I decided it would

be a great idea to continue my education here at Albany Medical College.

Giesse Albeche Duarte

I got my first master's degree in Rehabilitation Science in 2015 from UFCSPA in Brazil. I

decided to pursue a graduate degree in Basic Sciences because, from my clinical experi-

ence, I found there is a lack of scientific and medical information available for treat-

ments of diseases and I want to contribute to this field.

Hannah Fitzgerald I graduated from SUNY Cortland this spring with a degree in Biomedical Science and a

minor in Chemistry. My plans after graduation are to attend medical school and pursue

service abroad. I chose AMC because I loved the community created by the faculty at

the college and the opportunities provided to students at every level. I look forward to a

incredible learning experience.

Catherine Sherry - Ph.D. Program

I plan on continuing with research as a post doc. As an undergrad I attended Clarkson

University where I got my degree in bio-molecular science in 2015, then to Albany Col-

lege of Pharmacy where I got a masters degree in clinical laboratory science in 2017. I

chose this program because I've always found biochemistry, molecular biology, and ge-

netics very interesting and I loved the idea of applying my curiosity in a way that helps

people.

Neelam Maheshwari– Ph.D. Program

I am a Pakistani native. I graduated from Liaquat University of Medical and Health Sci-

ences in Jamshoro Pakistan, with a Bachelors of Medicine and Bachelors of Surgery

(MBBS) in December 2017. I joined a lab in Karachi Pakistan after my one-year clinical

internship to get research experience. This experience helped me choose a career in re-

search over a career in clinical. Dr. Dale Tang research area piqued my interest, there-

fore I chose Molecular and Cellular Physiology Ph.D. program at Albany Medical College.

MCP Newsletter 3

Publications

Ajay P. Nayak, John Lim, Eylon Arbel, Ruping Wang, Dominic R. Villalba, Tahn L. Nguyen, Niccole Schai-ble, Ramaswamy Krishnan, Dale Tang, Raymond Penn. -Abl in-hibitors in regulating airway smooth muscle relaxation. FASEB Journal. 2021;35:e21674 Wang Y, Liao G, Wang R, Tang DD. Acetylation of Abelson interactor 1 at K416 regulates actin cytoskele-ton and smooth muscle contraction. FASEB Journal. 2021;35:e21811 Kenney J, Ndoye A, Lamar JM, DiPersio CM: Comparative use of CRISPR and RNAi to modulate integrin α3β1 in triple negative breast cancer cells reveals that some pro-invasive/pro-metastatic α3β1 functions are independent of global regulation of the transcriptome. PLOS ONE 16(7): e0254714, 2021. [PMID:34270616; PMCID:PMC8284828] Longmate WM, Miskin RP, Van De Water L, DiPersio CM: Epidermal integrin α3β1 regulates tumor-derived proteases BMP-1, MMP-9 and MMP-3. JID Innov, in press Lajoie JE, Lau KW, Adam AP, Wladis EJ. Cytotoxic T-Lymphocyte-Associated Protein-4 and Lymphocyte Activation Gene-3 Expression in Orbitally-Invasive Versus Nodular Basal Cell Carcinoma. Ophthalmic Plast Reconstr Surg. 2021 37(3S):S109-S111. Wladis EJ, Arunachalam T, LaJoie JE, Lau KW, Adam AP. Myeloid differentiation factor 88 expression in eyelid specimens of rosacea. Orbit. 2021 Mar 31;:1-6. Bossardi Ramos R, Adam AP. Molecular Mechanisms of Vascular Damage During Lung Injury. Adv Exp Med Biol. 2021;1304:95-107 Martino N, Bossardi Ramos R, Lu S, Leyden K, Tomaszek L, Sadhu S, Fredman G, Jaitovich A, Vincent

P, Adam AP. Endothelial SOCS3 maintains homeostasis and promotes survival in endotoxemic mice. J Clin Invest Insight 2021 6(14):147280 Seavey C.N., Pobbati A.V., Hallett A., Ma S., Reynolds J.P., Kanai R., Lamar J.M., Rubin B.P. WWTR1(TAZ)-CAMTA1 gene fusion is sufficient to dysregulate YAP/TAZ signaling and drive epithelioid hemangi-oendothelioma tumorigenesis. Genes Dev. 2021 Apr 1;35(7-8):512-527 (Epub) PMID: 33766982 Sadhu S, Decker C, Sansbury BE, Marinello M, Seyfried A, Howard J, Mori M, Hosseini Z, Arunachalam

T, Finn AV, Lamar JM, Jourd’heuil D, Guo L, MacNamara KC, Spite M, Fredman G. Radiation-induced macrophage senescence impairs resolution programs and drives cardiovascular inflammation. J Immunol. 2021.

Decker C, Sadhu S, Fredman G. Pro-Resolving Ligands Orchestrate Phagocytosis. Front Immunol. 2021 Jun 10;12:660865. doi: 10.3389/fimmu.2021.660865. eCollection 2021. (PMID: 34177900). Kasikara C, Schilperoort M, Gerlach B, Xue C, Wang X, Zheng Z, Kuriakose G, Dorweiler B, Zhang H, Fredman G, Saleheen D, Reilly MP, Tabas I. Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis. J Clin Invest. 2021 Apr 15;131(8):e145275. doi: 10.1172/JCI145275

Imaging from Cells to Animals In Vivo” (2020), New York: Barroso, M. (Ed.) and Intes, X. (Ed.), Chapman and Hall/CRC.

Dmitriev, R.I.; Intes, X.; Barroso, M. Luminescence lifetime imaging of three-dimensional biological ob-jects. J. Cell Sci. 2021, May 1;134(9):1-17.

PAGE 4 MCP NEWSLETTER

Publications

Tubbesing, K.; Khoo, T.C.; Bahreini, S.; Sharikova, A.; Barroso, M.; Khmaladze, A. Iron-binding cellular profile of transferrin using label-free Raman hyperspectral imaging and singular value decomposition (SVD). Free Radic. Biol. Med. 2021, 169, 416–424. Roberge, C.L.; Kingsley, D.M.; Faulkner, D.E.; Sloat, C.J.; Wang, L.; Barroso, M.; Intes, X.; Corr, D.T.

NonDestructive Tumor Aggregate Morphology and Viability Quantification at Cellular Resolution, During De-velopment and in Response to Drug. Acta Biomater. 2020, 117, 322–334.

Rudkouskaya, A.; Smith, J.T.; Intes, X.; Barroso, M.; Xavier, I. Quantification of Trastuzumab-HER2 en-gagement in vitro and in vivo. Molecules 2020, 25, 5976, doi:10.3390/molecules25245976 January Weiner, Phillip Suwalski, Manuel Holtgrewe, Alexander Rakitko, Charlotte Thibeault, Melina Müller, Dimitri Patriki, Claudia Quedenau, Ulrike Krüger, Valery Ilinsky, Iaroslav Popov, Joseph Balnis, Ariel Jaitovich, et al. Increased risk of severe clinical course of COVID-19 in carriers of HLA-C*04:01 EClin-icalMedicine, 2021,101099, ISSN 2589-5370, https://doi.org/10.1016/j.eclinm.2021.101099. Juan Guardela B, Sun J, Zhang T, Xu B, Balnis J, Huang Y, Ma SF, Molyneaux PL, Maher TM, Noth I, Michaud G, Jaitovich A, Herazo-Maya JD. 50-gene risk profiles in peripheral blood predict COVID-19 out-comes: A retrospective, multicenter cohort study EBioMedicine,Volume 69, 2021, 103439, ISSN 2352-3964 doi.org/10.1016/j.ebiom.2021.103439 Balnis J, Madrid A, Hogan KJ, Drake LA, Chieng HC, Tiwari A, Vincent CE, Chopra A, Vincent PA, Robek

MD, Singer HA, Alisch RS, Jaitovich A. Blood DNA methylation and COVID-19 outcomes. Clin Epigenetics. 2021 May 25;13(1):118. doi: 10.1186/s13148-021-01102-9. PMID: 34034806; PMCID: PMC8148415. Jaitovich A. Hypercapnic Respiratory Failure-Driven Skeletal Muscle Dysfunction: It Is Time for Animal Model-Based Mechanistic Research. Adv Exp Med Biol. 2021;1303:129-138. doi: 10.1007/978-3-030-63046-1_8. PMID: 33788191. Joseph Balnis , Lisa A Drake , Catherine E Vincent , Tanner C Korponay , Diane V Singer , David Laco-mis , Chun Geun Lee , Jack A. Elias , David Jourd'heuil , Harold A Singer , and Ariel Jaitovich ATS Jour-nal Of Respiratory Cell and Molecular Biology PMID: 33909984 DOI: 10.1165/rcmb.2020-0551OC Succinate Dehydrogenase (SDH)-subunit C Regulates Muscle Oxygen Consumption and Fatigabil-ity in an Animal Model of Pulmonary Emphysema

Dai W, Choubey M, Patel S, Singer HA, Ozcan L. Adipocyte CAMK2 deficiency improves obesity-associated glucose intolerance. Mol Metab. 2021 Jul 22;53:101300. doi: 10.1016/j.molmet.2021.101300. Epub ahead of print. PMID: 34303021; PMCID: PMC8365526. Balnis J, Drake LA, Vincent CE, Korponay TC, Singer DV, Lacomis D, Lee CG, Elias JA, Jourd'heuil D, Singer HA, Jaitovich A. SDH Subunit C Regulates Muscle Oxygen Consumption and Fatigability in an Ani-mal Model of Pulmonary Emphysema. Am J Respir Cell Mol Biol. 2021 Sep;65(3):259-271. doi: 10.1165/rcmb.2020-0551OC. PMID: 33909984. O'Brien BJ, Singer HA, Adam AP, Ginnan RG. CaMKIIδ is upregulated by pro-inflammatory cytokine IL-6 in a JAK/STAT3-dependent manner to promote angiogenesis. FASEB J. 2021 Apr;35(4):e21437. doi: 10.1096/fj.202002755R. PMID: 33749880; PMCID: PMC8223739.

MCP Newsletter 5

Grants

Dr. Antonio Paul $3.3M NIH Grant to investigate circadian disruption-induced cardi-ometabolic diseases

Continued circadian disruption, such as in over 8.6 million Ameri-can shift workers, is strongly associated with obesity, insulin re-sistance, and type 2 diabetes, and significantly increases the rates of coronary and carotid atherosclerosis and subsequent vascular events such as myocardial infarction and stroke. Antoni Paul, PhD, associ-ate professor at MCP, is part of a multidisciplinary team of 3 PIs with complementary expertise in molecular clock and diabetes (Vijay Yechoor, MD, University of Pittsburgh), light and circadian biology (Mariana Figueiro, PhD, Mount Sinai) and atherosclerosis (Dr. Paul)

that are investigating the mechanisms linking shift work and cardiometabolic diseases and testing phar-macological and environmental (lighting) preventive interventions.

Dr. Yong-Xiao Wang $326,000 R03 grant from the National Institute on Aging to study cerebral vascular calcium signaling in diabetic vascular contributions to cognitive impairment and de-mentia (VCID).

Dr. Yong-Xiao Wang was recently awarded a 2-year R03. The award is aimed at demonstrating the potential important role of cerebral vascular Rieske iron-sulfur pro-tein-mediated mitochondrial reactive oxygen species (ROS) in VCID and FK506 bind-ing protein 12.6/type-2 ryanodine receptor calcium release channel complex as a downstream molecule of the mitochondrial ROS.

Dr. Dale Tang $1.9 million NIH R01 grant to investigate the role of an intermediate filament pro-tein in airway smooth muscle function and asthma pathogenesis.

Dr. Dale Tang, Professor of Department of Molecular and Cellular Physiology, has re-cently been awarded a 4-year NIH R01, this is a competitive R01 renewal. Dr. Tang’s research is focused on the role and mechanisms of cytoskeleton-associated proteins and genes in smooth muscle, asthma, and hypertension. His research has been fund-ed by multiple NIH R01 grants.

Dr. Margarida Barroso

NIH 3R01 CA250636-02S1, NCI Innovative Molecular and Cellular Analysis Technol-ogies Supplement Program. Title: In vivo Macroscopic Fluorescence Lifetime Molecu-lar Optical Imaging; MPIs: Intes (Primary, RPI); Barroso (AMC); Williams (City of Hope). NIH R50, NCI Research Specialist Award, Title: Quantitative Imaging of Target En-gagement in Breast Cancer Models; PI: Dr. Rudkouskaya;

Dr. Alejandro Adam

Manhattan Eye Foundation. SHP2 in ocular rosacea. PI: Apoorv Chebolu. Role: co-mentor.

MCP Newsletter 6

Congratulations to Dr. Harold Singer who was presented with the 2021Jiri Turinsky

Outstanding Research Mentor Award.

Highlights

Dr. Ramon Bossardi Ramos

postdoctoral fellow at the Adam Lab at MCP, was awarded the early-career investigator scholarship for Columbia University’s Quantitative Genomics Training. Ramon will par-ticipate in seminars and hands-on analytical sessions for whole-genome and transcrip-tome analyses in human health studies. This scholarship will provide tools for Ramon’s research projects that involve the identification of the endothelial transcriptional pro-gram in response to severe inflammation.

Dr. Ramon Bossardi Ramos for receiving an ASIP 2021 Marion and Lawrence (Larry) Muller Trainee Scholar Award for Excellence in Inflammation Research!

John Lamar co-organized the fourth annual TSRC scientific meeting focused on targeting YAP/TAZ-TEAD in cancer. The workshop, which is held in Telluride, Colorado, is attended by many of the leaders in the Hippo-YAP/TAZ-TEAD field.

Both John Lamar and Ryan Kanai gave invited talks at the meeting.

John Lamar was invited to speak at the virtual EHE360 Scien-

tific Conference held January 2021. Talk title: Identification of negative regulators of the TAZ-CAMTA1 fusion protein focused mostly on work from Ryan Kanai.

Dr. Whitney Longmate

was invited to present an interactive poster (short talk) at the 2021 Annual Meeting of the Society for Investigative Dermatology (May 3-8, 2021; virtual meeting). Talk title: Epi-

dermal integrin α3β1 promotes a tumor-supportive protease secretome

Dr. Ramon Bossardi Ramos and Shuhan Lu

Post Doctorate Fellow and PhD student in the Adam lab, earned two Histochemical

Society EB Trainee Awards in recognition to their abstracts submitted for Experi-

mental Biology 2021. Both were also selected for virtual Minisymposia oral presenta-

tions.

MCP Newsletter 7

Masharh Lipscomb

Masharh recently received a NHLBI Research Diversity Supplement.

Ariel Jaitovich Technology Networks credited pulmonologist Ariel Jaitovich MD, with the foresight that triggered one of the first large-scale multi-omic analyses of COVID-19. Dr. Jai-tovich’s research, in collaboration with his AMC colleagues from the department of medicine, MCP and IMD, University of Wisconsin-Madison and the Morgridge Insti-tute for Research, identified more than 200 molecular features that drive the sever-ity of COVID-19 infections. Their findings were published in the journal Cell Sys-tems last fall.

Highlights

Dr. Gabrielle Fredman Invited Speaker 10/2022; 17th Int'l Conf. on Bioactive Lipids in Cancer 01/2022; LSU Health Shreveport, Center for Cardiovascular Diseases 12/2021; The 44th Annual Meeting of the Molecular Biology Society of Ja-pan

NIH study sections:

10/2021 Reviewer for CMAD (Cellular Mechanisms of Aging and De-

velopment)

06/2021 Reviewer: Special emphasis panel, NIH-NIEHS, Panel for RFA

-ES-20-013

Dr. Alejandro Adam

Grant review:

May 2021 AHA CDA Vascular Sciences 3 Committee

June 2021 Ad hoc reviewer SAT study section

Standing member, MRAC study section, starting in November 2021

Trainee presentations:

Role of endothelial SOCS3 in leukoembolization (Shuhan Lu presenter). Ex-

perimental Biology April 2021

SOCS3 Limits Pro-Inflammatory Signature in Septic Endothelium (Ramon

Bossardi Ramos presenter). Experimental Biology April 2021

MCP Newsletter 8

Highlights

Alena Rudkouskaya Ph.D.

Invited oral presentations “Multiscale quantification of drug-target engagement

in breast cancer models using Fluorescent Lifetime FRET imaging”, Capital Re-

gion Cancer Research New Frontiers Symposium 2021: Precision Medicine in

Cancer Treatment and Prevention, invited oral presentation.

Ling Wang, Ph.D.

Invited oral presentations “Organelle topology is a new breast cancer cell classi-

fier”, European Light Microscopy Initiative 2021 meeting virtual, poster and

flash talk presentation.

Jonathan Barra Ph.D.

Invited oral presentations “Iron transporter DMT1 regulates endosome-

mitochondria dynamics, mitochondrial metabolism and invasive migration in

breast cancer cells". Experimental Biology meeting 2021. Oral presentation.

Dr. Margarida Barroso

Society Memberships

Community Champion, SPIE (2020-2021)

Community Champions are SPIE Members who are recognized for the work

they do annually to both give back to the optics and photonics community and

advocate for SPIE.

Approximately 10 percent of Members are awarded the Community Champion

distinction every year.

Past-Past President, Histochemical Society (2021-2022)

Councilor, International Federation of Societies for Histochemistry and Cytochemistry (IFSHC) (2019-

present)

MCP Newsletter 9 Questions or Comments please contact Christina Schools at Schoolc@amc.edu

MCP Seminar Speaker

DEPARTMENT OF MOLECULAR &

CELLULAR PHYSIOLOGY

of

ALBANY MEDICAL C

Presents

Anthony Koleske Ph.D.

Ensign Professor,

Molecular Biophysics and Biochemistry of

Neuroscience,

Deputy Dean for Research

Basic Science Departments

Yale University of Medicine

Thursday, October 14, 2021

MS Teams, 9:00 a.m. Host: Dale Tang, Ph.D.

Dr. Anthony Koleske