Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield...

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Mastitis • Inflammation of the mammary gland • infection or injury • Important • reduced milk yield • increased culling • treatment cost & un-salable milk • lower milk quality

Transcript of Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield...

Page 1: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis

• Inflammation of the mammary gland

• infection or injury

• Important

• reduced milk yield

• increased culling

• treatment cost & un-salable milk

• lower milk quality

Page 2: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis

• Clinical

• acute or chronic

• abnormal milk, udder &/or cow appearance

• Subclinical

• can not detect by appearance

• somatic cells - epithelial cells & leukocytes

Page 3: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 4: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 5: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 6: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 7: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Milk Yield and SCCLact. Ave. Lact. Ave. Difference in yield (305d)

SCS SCC (1000/ml) Lact. 1 Lact. >1

0 12.5 - -

1 25 - -

2 50 - -

3 100 -200 -400

4 200 -400 -800

5 400 -600 -1200

6 800 -800 -1600

7 1600 -1000 -2000

Page 8: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 9: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis

• Contagious bacteria

• Staphylococcus aureus

• Streptococcus agalactiae

• Corynebacterium bovis

Page 10: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 11: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis

• Environmental bacteria• Escherichia coli• Klebsiella species• Citrobacter species• Enterobacter species• Streptococcus uberis, dysgalactiae,

equinus, & many others• Enterococcus faecalis & faecium

Page 12: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis

• Other mastitis pathogens

• Coagulase neg. staphylococci

• Pseudomonads

• Yeasts

• Mycoplasma species

Page 13: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 14: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Likelihood of New Infection

• Intensity of teat end exposure to pathogens (pathogen load)

• Likelihood of pathogens gaining entry to mammary gland

• Ability of pathogens to grow in mammary gland & survive host defense systems

Page 15: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 16: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Liner slip & Mastitis

0

10

20

30

40

Clinical Subclinical

High slip

Low slip

Page 17: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 18: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Air

Air - when liner slips

Air Air

Impacts

Droplet Impacts

Page 19: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Correlations between Udder Morphology & Liner SlipsCharacteristic Correlation

Front udder height -.23

Rear udder height -.28

Udder levelness .25

Front teat distance - before milking .44

Rear teat distance - before milking .34

Page 20: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Milking Speed & Mastitis (Phenotypic Relationship)

Slow Average flow rate Fast

Like

lihoo

d of

Mas

titis

Page 21: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Rates of New Infection by Stage of Lactation

Dry Calving Lactation Dry off

Immune suppressionRat

es o

f N

ew I

n fec

tion

Page 22: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 23: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Why New Infections Near Calving?

• Physiological stress associated with parturition

• Reduced concentration of nonspecific immune factors in secretions

• Edema & leaking of milk

• Components of colostrum that interfere with leukocyte function

Page 24: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Why New Infections at Beginning of Dry Period?

• Flushing of teat & ducts is terminated

• Increased udder pressure & leakage of milk

• Teat dipping & cleaning stopped

• Phagocyte function (immune cells) is impaired

Page 25: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Why Few New Infections During Middle of Dry Period?

• Low stress

• Decreased intramammary pressure

• Keratin plug

• Increased leukocyte concentration

• Increased concentrations of nonspecific immune factors (lactoferrin)

Page 26: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Exposure to Pathogens

• Contagious pathogens

• Reservoir - infected glands

• Milking equipment, hands, hygiene practices, teat conditions

• Environmental pathogens

• Exposure to teat surface is mostly between milkings

Page 27: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 28: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 29: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Detection & Diagnosis of Mastitis

• Herd level

• SCC

• Conductivity

• Bulk tank cultures

• Herd cultures

Page 30: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Detection & Diagnosis of Mastitis

• Cow level• Physical examination• Appearance of milk• California mastitis test (CMT)• SCC• Conductivity• Antibody tests (Staph. Aureus)• Individual cow/quarter cultures

Page 31: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 32: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 33: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 34: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

infected udder

Test tube to aseptically collect samples

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Possible infected udder

2 to 3 streams of foremilk

Page 36: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

.1 ml sample is drawn

Page 37: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 38: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 39: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Changes in Milk Composition Associated with Increased SCCConstituent Normal milk High SCC milk

SNF 8.9 8.8

Fat 3.5 3.2

Lactose 4.9 4.4

Total protein 3.6 3.6

Total casein 2.8 2.3

Whey protein .8 1.3

Page 40: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 41: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Changes in Milk Composition Associated with Increased SCCConstituent Normal milk High SCC milk

Serum albumin .02 .07

Lactoferrin .02 .10

Immunoglobulins .10 .60

Sodium .057 .105

Chloride .091 .147

Potassium .173 .157

Calcium .12 .04

Page 42: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 43: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 44: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Premilking preparation• Cleaning & drying teats

• Limit water & dry - use indiv. towel• Predipping

• Can help with environmental mastitis• Methods?• Hazards?

Page 45: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 46: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Teat dipping (post milking)

• Contagious pathogens mostly (50% reduction in new infections)

• Tested products only (Iodine or Chlorine based)

• Barrier dips

• Hazards/problems?

• Application methods?

Page 47: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Page 48: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Side spray does NOT cover all of teat touched by liner!!

Page 49: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 50: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Page 51: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Be sure to cover all the teat touched by the liner!!

Page 52: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 53: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Cup Dipping

Be sure to cover all the teat touched by the liner!!

Page 54: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Dry cow therapy• Treat all cows/quarters• Cures existing infections• Decreases new infections at drying off

(Strep. Ag. - not E. coli.)• Does not impact new infections at calving• Hazards/problems?• Application methods?

Page 55: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 56: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Partial Insertion

Short cannula Long cannula

Do NOT insert long cannula

all the way into the teat-will cause damage

to keratin

Page 57: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Insert cannula justthrough the streak canal

-further insertion may damage keratin

Page 58: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Segregation• Sick cows separate & milk last• Select replacements carefully

• Bedding• Clean & dry• Inorganic if possible• Stall design & housing• Bedded packs are a problem

Page 59: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 60: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 61: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 62: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Nutrition

• Vitamins (E is critical)

• Selenium (recommend .3 ppm)

• Keep cows standing after milking

• Feeding?

Page 63: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Vaccination

• Stimulate antibodies against mastitis

• Problem - many strains

• Coliform - J5 vaccines

• Reduces number of clinical events & severity (not fewer infections)

• Other vaccines (Staph. Aureus)

Page 64: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis Therapy

• Antibiotics

• 10-12 over the counter drugs

• 5-6 prescription drugs

• Extra-label use (need Rx)

• Careful with residues (withdrawl)

• Intramammary infusion or under skin

Page 65: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis Therapy - When & How to Use

• Subclinical mastitis (SCC or conductivity)?

• Clinical mastitis

• Know pathogens involved - critical

• Historical record is critical to success

• Severity is important

Page 66: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis Therapy - When & How to Use

• Coliform mastitis

• Acute or peracute - vet or intense treatment including fluid & anti-inflammatory

• Mild to moderate - oxytocin & milking

• Antibiotic therapy - not indicated

• Problems/issues?

Page 67: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 68: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 69: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis Therapy - When & How to Use

• Staph. aureus

• Antibiotic therapy - try initially

• Problems/issues?

• Strep. agalactiae

• Antibiotic therapy - usually works

• Problems/issues?

Page 70: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Mastitis Therapy - When & How to Use

• Strep. other than Strep. agalactiae

• Antibiotic therapy - usually indicated

• Problems/issues?

• Other species?

Page 71: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Heifer Mastitis

• More prevalent than most people think

• >50% of quarters infected

• Coag. neg. staph.• Strep. other than Strep. Agalactiae

• Staph. Aureus

• Antibiotic therapy before calving?

Page 72: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

No. Klebsiella Bacteria per ml of Bedding

Chopped straw

Sawdust

Sand

Limestone

100K

10K

1K

100

Page 73: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Straw

Sawdust

Corncobs

Limestone

Sand

No. Coliform Bacteria per ml of Bedding

10m

1m

100K

10K

100

Page 74: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Blood agar plate

Sample is smeared on a blood agar plate

Page 75: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Blood agar plate 1 per quarter

Mastitis-causing bacteria colonies

Page 76: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Streptococcus

Page 77: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Staphylococcus Aureus

Page 78: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Coliforms, and other gram negative

organisms

Page 79: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Control of Mastitis

• Backflushing

• Contagious pathogens

• Necessary?

Page 80: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Be sure to cover all the teat touched by the liner!!

Page 81: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Page 82: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Spraying

Page 83: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 84: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Cup Dipping

Page 85: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Cup Dipping

Page 86: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Cup Dipping

Page 87: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Cup Dipping

Page 88: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Teat Dipping Techniques

Cup Dipping

Be sure to cover all the teat touched by the liner!!

Page 89: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 90: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 91: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 92: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Streak canal

Sphincter muscle

Page 94: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 95: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 96: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 97: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 98: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.
Page 99: Mastitis Inflammation of the mammary gland infection or injury Important reduced milk yield increased culling treatment cost & un-salable milk lower milk.

Detacher Setting Flow Rates & Left Over Milk

0

0.5

1

1.5

.5 lbs./min

2.0 lbs./min

Left

Ove

r M

ilk in

lbs.