MAS.S62 FAB 2 4.3.12 Self Replicating Biochemical Systems
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Transcript of MAS.S62 FAB 2 4.3.12 Self Replicating Biochemical Systems
MAS.S62 FAB2
4.3.12
Self Replicating Biochemical Systems
Holliger et. al
Threshold for LifeWhat is the Threshold for Self Replicating Systems?
Measurement Theory
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Step 1 Step 2 Step 3
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Parts
Template
Machine
Replication Cycle
http://en.wikipedia.org/wiki/File:Stem-loop.svg
Error Correcting Exonuclease
(Ruler)
DNA
Number of Nucleotides
Prob
abili
ty o
f Sel
f Rep
licati
on
NN
N
N
kT
qp
qQp
kq
q
N
Bond/E-
-1 P :Yield Total
11 :Yield StepPer
:open bonds N ally that Probabilit
3E e :Where
:open is bond single ay that ProbabilitBond
Watson Crick .18 nm
How Well Can N Molecules Measure Distance?
/sandwalk.blogspot.com/2007/12/dna-denaturation-and-renaturation-and.html
200 400 600 800 1000 1200 1400
0.2
0.4
0.6
0.8
1.0
Threshold length: 691 bp for 50% yield. 315 bp for 10-6 yield.
220 ntCombinatorics1:1E132Library Diversity1:1E15
Pligase = 1:2E13
Minimum ribozyme length 165 nt14 nt extensionError rate: 1%Extension rate : 1nt per 153 minutes
Minimum ribozyme length:20 nt extensionError rate: Extension rate :
95 nt base extension