Mass Spectrometry Sample Preparation Tools Thermo ... · extraction and collection. Our sample...
Transcript of Mass Spectrometry Sample Preparation Tools Thermo ... · extraction and collection. Our sample...
Mass Spectrometry Sample Preparation ToolsTo ensure the best possible analytical results, it remains crucial to have your protein samples in the right chemical environment and at a proper concentration after extraction and collection. Our sample preparation tools provide quick handling and exceptional recovery for small to bigger volumes. Find the best product to suit your needs in our broad portfolio of products for dialysis, desalting, concentration, ion exchange, etc.
Dialysis
Do you need to dialyze large-volume samples and do you want to avoid the hassle of loading your sample into slippery tubing? If so, pre-opened dry Thermo Scientific™ SnakeSkin™ Dialysis Tubing will work for you.Sample volume: 10-100 mL
SnakeSkin Dialysis Tubing
Completely remove salts or other small molecules from your precious low-volume sample using Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Units. The very convenient and safe devices provide maximal sample recovery.Sample volume: 10 μL-2 mL
Slide-A-Lyzer MINI Dialysis Units
If you need to completely remove salts or other small molecules from your sample, Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes are a convenient and safe device and they provide maximum sample recovery.Sample volume: 0,1-30 mL
Slide-A-Lyzer Cassettes
The Thermo Scientific™ Slide-A-Lyzer™ G2 Dialysis Cassettes offer easy sample loading and removal by using a serological pipette. The built-in air chamber provides buoyancy and vertical orientation of the cassette during dialysis. The new Thermo Scientific™ Slide-A-Lyzer™ Dialysis Flasks are designed to process sample volumes of 70 to 250 mL.Sample volume: 0,1-250 mL
Slide-A-LyzerG2 DialysisCassettes and Flasks
Desalting
Concentration
Quickly remove salts and small molecules from your various protein solutions with Thermo Scientific™ Zeba™ Spin Columns and Plates. The easy-to-handle devices provide exceptional protein recovery.Sample volume: 2 μL-4 mL
Zeba Spin Columns
The unique design of Thermo Scientific™ Pierce™ Concentrators provides the best solution for concentrating your protein without losing sample. Concentrators with a polyethersulfone (PES) membrane are also available.Sample volume: 1-20 mL
Concentrators Concentrators PES
Desalt your sample in a cost-efficient way using regenerable Thermo Scientific™ Desalting Columns available with different gels to best suit your protein’s properties.Sample volume: 0,2-1 mL
Polyacrylamide and Dextran Columns
Do you need to pre-fractionate or purify your target protein from a crude biological sample? Thermo Scientific™ Pierce™ Strong Ion Exchange Columns enable rapid protein fractionation and sample preparation based on charge differences. Choose between mini or maxi size and anionic or cationic format of these columns.Sample volume: mini up to 500 μL and maxi up to 20 mL
Strong Ion Exchange Spin Columns
www.thermoscientific.com/pierce
you have the samples we have the tools
Proper sample preparation for mass spectrometry-based analysis is a critical step in the proteomics workflow. The quality and reproducibility of sample extraction and preparation for downstream analysis significantly impacts the separation and identification capabilities of mass spectrometers.
• C18 Tips, Spin Tips and Spin ColumnsThermo Scientific™ Pierce™ C18 Tips are ready-to-use pipette-tip columns of C18 resins. Thermo Scientific™ Pierce™ C18 Spin Columns are ready-to-use centrifuge columns of porous C18 resins. C18 resins enable fast and efficient capture, concentration, desalting and elution of peptides for MALDI mass spectrometry and other methods. The treatable sample volume varies from 10 to 150 µL according to the type of tips or columns used.
• Graphite Spin Columns Thermo Scientific™ Pierce™ Graphite Spin Columns improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before mass spectrometry analysis. The sample volume that can be processed is up to 500 µL per column.
• Abundant Protein Depletion Spin Columns Thermo Scientific™ Pierce™ Abundant Protein Depletion Spin Columns are optimized to decrease the abundant albumin and antibody components during the preparation of human plasma samples for mass spectrometry. Two resin options for top two or top 12 abundant protein depletion are available.
• Detergent Removal Spin Columns and Plates Thermo Scientific™ Pierce™ Detergent Removal Resins efficiently bind to and remove high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss and are available prepacked into spin columns and 96-well spin filter plates.
• HiPPR Detergent Removal Resin, Columns and Plates Thermo Scientific™ HiPPR Detergent Removal Resins used in spin column or 96-well filter plate formats improve mass spectrometry results by efficiently removing detergents from 25 to 200 µL samples with low protein or peptide concentrations.
Thermo Scientific Sample Clean-up Products
Thermo Scientific Sample Clean-up Products
© 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries. PB_2012_84
Africa /Belgium/Europe/Middle East +32 53 85 71 84France 0800 50 82 15Germany 0228 9125650Netherlands 076 50 31 880Switzerland 0800 56 31 40 UK 0800 252 185
Email: [email protected] www.thermoscientific.com/pierce
For other regions, visit www.thermoscientific.com/piercedistributors
USA +815-968-0747 or +800-874-3723 Customer Assistance E-mail:[email protected] www.thermoscientific.com/pierce
G2 Dialysis Cassettes and Flasks
Thermo Scientific™ SnakeSkin™ Dialysis Tubing 3,5K MWCO:
88242 16 mm 35 ft. (10,5 m)
68035 22 mm 35 ft. (10,5 m)
88244 35 mm 35 ft. (10,5 m)
SnakeSkin Dialysis Tubing 7K MWCO:
68700 22 mm 35 ft. (10,5 m)
SnakeSkin Dialysis Tubing 10K MWCO:
88243 16 mm 35 ft. (10,5 m)
68100 22 mm 35 ft. (10,5 m)
88245 35 mm 35 ft. (10,5 m)
SnakeSkin Dialysis Accessories:
68011 Clips 6 clips
Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Device, 2K MWCO:
69580 0,1 mL 50 devices
69553 0,1 mL 250 devices
Slide-A-Lyzer MINI Dialysis Device, 3,5K MWCO:
69550 0,1 mL 50 devices
69552 0,1 mL 250 devices
88400 0,5 mL 25 devices
88403 2 mL 25 devices
Slide-A-Lyzer MINI Dialysis Device, 7K MWCO:
69560 0,1 mL 50 devices
69562 0,1 mL 250 devices
Slide-A-Lyzer MINI Dialysis Device, 10K MWCO:
69570 0,1 mL 50 devices
69572 0,1 mL 250 devices
88401 0,5 mL 25 devices
88404 2 mL 25 devices
Slide-A-Lyzer MINI Dialysis Device, 20K MWCO:
69590 0,1 mL 50 devices
69555 0,1 mL 250 devices
88402 0,5 mL 25 devices
88405 2 mL 25 devices
Slide-A-Lyzer MINI Dialysis Device Floats:
69588 25-unit capacity 4 floats
Thermo Scientific™ Protein Concentrators PES:
88512 3K MWCO, 0,5 mL 25 units
88513 10K MWCO, 0,5 mL 25 units
88502 30K MWCO, 0,5 mL 25 units
88503 100K MWCO, 0,5 mL 25 units
Thermo Scientific™ Protein Concentrators, 9K MWCO:
87748 7 mL 10 units
89884A 7 mL 25 units
87749 20 mL 10 units
89885A 20 mL 25 units
Protein Concentrators, 20K MWCO:
87750 7 mL 10 units
89886A 7 mL 25 units
87751 20 mL 10 units
89887A 20 mL 25 units
Protein Concentrators, 150K MWCO:
89920 7 mL 10 units
89922 7 mL 25 units
89921 20 mL 10 units
89923 20 mL 25 units
Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO:66205 0,5 mL 10 cassettes
66203 3 mL 10 cassettes
66212 12 mL 8 cassettes
66230 30 mL 6 cassettes
Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 3,5K MWCO:66333 0,5 mL 10 cassettes
66330 3 mL 10 cassettes
66110 12 mL 8 cassettes
66130 30 mL 6 cassettes
Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 7K MWCO:66373 0,5 mL 10 cassettes
66370 3 mL 10 cassettes
66710 12mL 8 cassettes
Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 10K MWCO:66383 0,5 mL 10 cassettes
66384 0,5 mL 5 x 10 cassettes
66380 3 mL 10 cassettes
66381 3 mL 5 x 10 cassettes
66810 12 mL 8 cassettes
66811 12 mL 5 x 8 cassettes
66830 30 mL 6 cassettes
Sterile - Gamma irradiated Slide-A-Lyzer Dialysis Cassettes: check website
Slide-A-Lyzer Dialysis Cassettes, 20K MWCO:66005 0,5 mL 10 cassettes
66003 3 mL 10 cassettes
66012 12 mL 8 cassettes
66030 30 mL 6 cassettes
Thermo Scientific™ Slide-A-Lyzer™ G2 Dialysis Cassettes and Flasks, 2K MWCO:87717 0,5 mL 10 cassettes
87718 3 mL 10 cassettes
87719 15 mL 8 cassettes
87720 30 mL 6 cassettes
87721 70 mL 6 cassettes
87760 250 mL 4 flasks
Slide-A-Lyzer G2 Dialysis Cassettes, 3,5K MWCO:87722 0,5 mL 10 cassettes
87723 3 mL 10 cassettes
87724 15 mL 8 cassettes
87725 30 mL 6 cassettes
87726 70 mL 6 cassettes
87761 230 mL 4 flasks
Slide-A-Lyzer G2 Dialysis Cassettes, 7K MWCO:87727 0,5 mL 10 cassettes
87728 3 mL 10 cassettes
Slide-A-Lyzer G2 Dialysis Cassettes, 10K MWCO:87729 0,5 mL 10 cassettes
87730 3 mL 10 cassettes
87731 15 mL 8 cassettes
87732 30 mL 6 cassettes
87733 70 mL 6 cassettes
87762 250 mL 4 flasks
Sterile - Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes: check website
Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 20K MWCO:87734 0,5 mL 10 cassettes
87735 3 mL 10 cassettes
87736 15 mL 8 cassettes
87737 30 mL 6 cassettes
87738 70 mL 6 cassettes
87763 250mL 4 flasks
Slide-A-Lyzer Flotation Disk:87759 Slide-A-Lyzer Flotation Disks 6 disks
Thermo Scientific™ Pierce™ Strong Cation Exchange Spin Column:
90008 Mini 24 columns
90009 Maxi 8 columns
Thermo Scientific Pierce™ Strong Anion Exchange Spin Column:
90010 Mini 24 columns
90011 Maxi 8 columns
Dialysis Desalting Concentration Dialysis Tubing and Clips Mini Dialysis Devices Dialysis Cassettes
Strong Ion Exchange Spin Columns
• Convenient – ready-to-use, pre-opened, pre-wetted and pleated tube
• High recovery – greater than 90% protein recovery
• Speed – dialysis is generally completed in four to six hours
• Stability – compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols
• Three convenient sizes – dialysis devices for 0,1; 0,5 and 2 mL samples fit standard 1,5mL-microcentrifuge, 15 mL and 50 mL-conical tubes, respectively
• Five membrane cut-offs – color coded to easily find the one you want 2K; 3,5K; 7K; 10K and 20K
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• 100% leak-tested – innovative design does not permit “wicking” that can occur in homemade devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
• Five color-coded membrane cut-offs and volume sizes – select the cassette that best suits your sample volume
• Thickness – 0,9 to 1,2 mL (22,5 to 30 µm)
• High recovery – rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Fast dialysis – flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• Easy to use – no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Superior design – thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple cassette sizes – select the cassette that best suits your sample volume
• Easy loading – pipette-accessible for easy sample loading and retrieval
• Self-floating – integrated air chambers eliminate the need for float buoys (cassettes only)
Thermo Scientific™ Polyacrylamide Desalting Columns 1,8K MWCO:
43426 5 mL 5 columns
Polyacrylamide Desalting Columns 6K MWCO:
43240 5 mL 5 columns
43243 10 mL 5 columns
Polyacrylamide Spin Desalting Columns 7K MWCO:
89849 0,7 mL 25 columns
89862 0,7 mL 50 columns
Thermo Scientific Dextran Desalting Columns 5K MWCO:
43230 5 mL 5 columns
43233 10 mL 5 columns
Polyacrylamide and Dextran Columns
• Chemical stability – stable in water, salt solutions, organic solvents and alkaline or acidic conditions
• Flow rate – approx. 1mL/min (relatively fast compared to other desalting resins)
• Heat stability – autoclavable dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties
Concentrators
• Rapid protein concentration – achieve greater than 100-fold protein concentration in less than 45 minutes
• Excellent protein recovery – remove buffer and small molecules while retaining more than 90% of proteins larger than 9kDa
• Convenient – two sizes designed to fit 15 mL and 50mL conical tubes; ideal for simple concentration of 1 to 20 mL of sample
• Instrument-compatible – use standard centrifuges with swinging-bucket or fixed-angle rotors; collect and recover sample without invert spinning
• Versatile – the easiest way to perform sample concentration, diafiltration or buffer-exchange of biological samples
Concentrators PES
• Rapid protein concentration – sample con-centration of 10- to 30-fold can be achieved in 10 minutes or less
• Excellent protein recovery – unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
• Instrument-compatible – use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
• Versatile – easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples
• Fast and simple – membrane-based spin format eliminates column packing
• Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel
• Robust – membrane adsorber spin columns do not crack or run dry
• Low bed volume – small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions
Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO:
89877 75 µL 25 columns
89878 75 µL 50 columns
89882 0,5 mL 25 columns
89883 0,5 mL 50 columns
89889 2 mL 5 columns
89890 2 mL 25 columns
89891 5 mL 5 columns
89892 5 mL 25 columns
89893 10 mL 5 columns
89894 10 mL 25 columns
Thermo Scientific™ Zeba™ 96-well Spin Desalting Plates, 7K MWCO:
89807 96-well Plates 2 plates
89808 96-well Plates 4 plates
Thermo Scientific™ Zeba™ Chromatography Cartridges, 7K MWCO:
89934 Zeba Desalting Chromatography Cartridges, 1 mL 5 cartridges
89935 Zeba Desalting Chromatography Cartridges, 5 mL 5 cartridges
Zeba Spin Desalting Columns, 40K MWCO:
87764 75 µL 25 columns
87765 75 µL 50 columns
87766 0,5 mL 25 columns
87767 0,5 mL 50 columns
87768 2 mL 5 columns
87769 2 mL 25 columns
87770 5 mL 5 columns
87771 5 mL 25 columns
87772 10 mL 5 columns
87773 10 mL 25 columns
Zeba 96-well Spin Desalting Plates, 40K MWCO:
87774 96-well Plates 2 plates
87775 96-well Plates 4 plates
Zeba Spin Columns
• High recovery – low-binding resin maximizes protein recovery
• Fast – no fraction screening or waiting for protein to emerge by gravity-flow
• Easy to use – no cumbersome column preparation or equilibration
• Flexible – available in spin columns, filter spin plates and cartridges for a range of needs
• Economical – great performance at lower cost than other commercially available cartridges