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1 UNO STAGE TOP INCUBATOR All In One Stage Top Incubator Manual Vers. 3-2019
Transcript of Manual - Okolaboko-lab.com/images/okaImages/PDF-files/UNO-STAGE-TOP-INCUBA… · UNO STAGE TOP...
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UNO STAGE TOP INCUBATOR
All In One Stage Top Incubator
Manual
Vers. 3-2019
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Index
Index PREFACE .............................................................................................................. 5
2 SYMBOLS DESCRIPTION ............................................................................................ 6
SYMBOLS IN THIS MANUAL AND ON THE PRODUCT ................................................................. 6 SYMBOLS ON THE PRODUCT LABEL ................................................................................. 6
3 SAFETY NOTES ........................................................................................................ 7
4 SYSTEM OVERVIEW ................................................................................................... 9
UNO CONTROL UNIT ............................................................................................. 9 4.1.1 BUNDLE 1) UNO-T. Overview and supplied equipment ............................................... 11 4.1.2 BUNDLE 2) UNO-T-H-PREMIXED. Overview and supplied equipment ............................. 12 4.1.3 BUNDLE 3) UNO-T-H-CO2. Overview and supplied equipment ...................................... 13
STAGE TOP CHAMBER ......................................................................................... 15
5 INSTALLATION GUIDE ............................................................................................... 15
ASSEMBLY AND GAS CONNECTION OF THE BUBBLING COLUMN ................................................. 15 CONNECTIONS OF THE HEATING DEVICES ......................................................................... 16
5.2.1 Connection of the Chamber and of the Lid of the Incubating Chamber .............................. 16 5.2.2 Connection of the Humidifier and of the Heated Tube .................................................. 17 5.2.3 Connection of the Objective Heater (optional) ........................................................... 18
CONNECTION OF THE GAS TUBES .................................................................................. 18 5.3.1 Gas connection for UNO-T-H-PREMIXED ............................................................... 18
5.3.1.1 How to use the GF Regulator .......................................................................... 19 5.3.2 Gas Connection for UNO-T-H-CO2 ........................................................................ 20
5.3.2.1 How to use the Air/CO2 Mixer ......................................................................... 20 CONNECTION OF THE TEMPERATURE SENSOR ................................................................... 22 MINI-USB PORT .................................................................................................... 23
5.5.1 Connection of a USB drive .................................................................................. 23 5.5.2 PC Connection via USB ..................................................................................... 23
RS232 PORT – PC CONNECTION VIA RS232 ..................................................................... 24 INSTALLATION OF THE STAGE TOP CHAMBER ..................................................................... 24
6 USER INTERFACE .................................................................................................... 25
HOMEPAGE .......................................................................................................... 25 6.1.1 Colors meaning ............................................................................................... 26 6.1.2 Changing the set point temperature ....................................................................... 26 6.1.3 Graph view ..................................................................................................... 26
SYSTEM INFORMATION .............................................................................................. 27 SETTINGS ............................................................................................................ 27
6.3.1 Control Mode .................................................................................................. 28 6.3.1.1 Open Incubator ........................................................................................... 30
6.3.2 Chamber & Insert ............................................................................................. 31 6.3.2.1 Compatible chambers ................................................................................... 32
6.3.3 Objective Heater .............................................................................................. 34 6.3.4 Humidity ........................................................................................................ 35 6.3.5 Calibration ..................................................................................................... 36
6.3.5.1 Chamber Calibration .................................................................................... 36 6.3.5.2 T sensor Calibration ..................................................................................... 37 6.3.5.3 Objective Heater Calibration ........................................................................... 39
6.3.6 Logging ......................................................................................................... 40 6.3.7 Alarms .......................................................................................................... 42 6.3.8 Display .......................................................................................................... 44
6.3.8.1 Options .................................................................................................... 44 6.3.8.2 Brightness ................................................................................................. 45 6.3.8.3 Calibration ................................................................................................ 45
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6.3.8.4 Date & Time .............................................................................................. 47 6.3.8.5 Visual Effects ............................................................................................. 47 6.3.8.6 Factory Reset ............................................................................................ 48
6.3.9 Password....................................................................................................... 48 6.3.10 Summary .................................................................................................... 49 6.3.11 Auto on/off ................................................................................................... 49
STATUS ............................................................................................................... 51 GLANCE MODE ...................................................................................................... 51
7 MAINTENANCE ........................................................................................................ 52
8 SUPPORT ............................................................................................................... 53
SUPPORT PAGE ...................................................................................................... 53 SUPPORT CONTACTS ............................................................................................... 54 WEB CONFERENCE FOR ASSISTANCE AND TRAINING ............................................................ 54
9 TECHNICAL SPECIFICATION ...................................................................................... 55
10 TROUBLESHOOTING .............................................................................................. 56
11 FIGURE LIST ......................................................................................................... 57
12 TABLE LIST .......................................................................................................... 58
13 MANUAL REVISION TABLE ...................................................................................... 59
14 LIST OF SPARE PARTS ........................................................................................... 60
UNO-T-H-PREMIXED .......................................................................................... 60 UNO-T-H-CO2 ................................................................................................... 60
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Preface
UNO STAGE TOP INCUBATOR is a Digital Temperature (and Humidity) Controller and a data log device
enabling the control of the environment inside the top stage incubator and is composed by UNO CONTROL
UNIT and a STAGE TOP CHAMBER.
UNO CONTROL UNIT ensures the highest temperature accuracy on sample temperature by controlling
simultaneously up to 6 independent channels with a modern multichannel controller.
UNO CONTROL UNIT comes in three different setups:
UNO-T
• UNO CONTROL UNIT
UNO-T-H-PREMIXED
• UNO CONTROL UNIT
• Humidity module
• Flow rate regulator for premixed gas
UNO-T-H-CO2
• UNO CONTROL UNIT
• Humidity module
• CO2/Air gas mixer
• Air Pump
The OBJECTIVE HEATER is an optional device which can be purchased upon request. It ensures the absence
of a heat sink when the microscope objective is in close contact with the specimen. Please contact Okolab
for more information on this device.
The end user can define the tolerance values, which can trigger an alarm.
UNO CONTROL UNIT allows you also to log your experimental data via USB connection (or RS232 connection),
using an OKOLAB software, DATALOG (purchased on request) or a third party software. Moreover, UNO
CONTROL UNIT can store data on its internal memory and these data can be then downloaded on a USB stick
at user request.
Reading this manual is essential to familiarize yourself with the installation, the functions and the operation
of the device before use.
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2 SYMBOLS DESCRIPTION
Symbols in this manual and on the product
The following symbols identify important information to note:
CAUTION or WARNING: This symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: This symbol warns you of circumstances or practices
that can affect the instrument’s functionality. Please refer to accompanying documents.
Tip - Supplies you with helpful suggestions.
Note - Supplies you with important information to successfully setup and use the instrument.
Symbols on the product label
CE MARKING: This symbol indicates a product’s compliance with EU legislation.
PRODUCT DISPOSAL: This symbol indicates that this product must not be disposed as urban
solid waste.
This symbol indicates the product production date
This symbol indicates the manufacturer data
This symbol indicates the protection degree against ingress of solids or liquids inside the
product.
This symbol warns you to read the user manual before starting the device
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3 SAFETY NOTES
Before operating the equipment please read carefully the instructions and the safety notes. If you have any
questions, please contact Okolab.
− The equipment must be used only as intended and described in this Manual.
− Equipment should be operated only by technically qualified personnel.
− Do not start up the equipment if some of its parts are damaged.
− This instrument is not intended for use in locations subject to flammable or explosive
gases.
− Transport the equipment with care.
− The equipment and its internal parts can be damaged by dropping and by shock.
− Do not use a volatile solvent such as paint thinner to clean the instrument. Deformation
or discoloration will occur. Use a soft, dry cloth to remove stains from the instrument.
Not following these instructions can damage or breakdown the device and its
accessories.
− The product labels can be found on the bottom panel of the Main Unit.
− Do NOT make in close contact with or breathe any gas stream whose composition is
different from that of ambient air.
− Do not exceed the voltage indicated in this manual and on the product label.
− Avoid excessive induction noise, static electricity, magnetic fields.
− Do not expose this instrument to water or moisture.
− Prevent throttling and kinking of tubing.
− Check tubing from time to time for possible wear and tear.
− Check that all tubing are well inserted into connectors so they cannot slip off.
− This device has not been designed for use for medical applications.
− Install safety valves and adequate pressure regulators on gas lines before the Unit input
connectors.
− The power cord of the unit should be unplugged from the electrical outlet when left
unused for a long time period.
− The unit should be placed far from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.
− Do not start up the equipment if the supply cable is damaged.
− Connect the equipment only to a grounded mains power socket.
− Do not disconnect cables while in operation.
− Prevent metal fragments or lead wire scraps from falling inside the instrument case to
avoid electric shock, fire or malfunction.
− Before starting, assemble the equipment far from any electrical devices.
− The unit should never be used where it can fall or be pushed into water.
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International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions can
result in damage or breakdown of the device and its accessories.
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES
OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY
PRODUCT.
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4 SYSTEM OVERVIEW
This Chapter describes the provided equipment. For your UNO STAGE TOP INCUBATOR that comes in three
different bundles:
Bundle 1) UNO-T. Stage Top Incubator for application where only thermal control is required (no
gas/no humidity), which includes (see paragraph 4.1.1):
a. UNO CONTROL UNIT
b. Stage Top Chamber
Bundle 2) UNO-T-H-PREMIXED. Stage Top Incubator for application with pre-mixed gas, which includes
(see paragraph 4.1.2):
a. UNO CONTROL UNIT
b. Humidity module
c. Flow rate regulator for premixed gas
d. Stage Top Chamber
Bundle 3) UNO-T-H-CO2. Stage Top Incubator for application with 100% CO2/Air mixer, for application
with 100% CO2 and background Air, which includes (see paragraph 4.1.3):
a. UNO CONTROL UNIT
b. Humidity module
c. CO2/Air gas mixer
d. Air Pump
e. Stage Top Chamber
UNO CONTROL UNIT
Figure 1 shows the UNO CONTROL UNIT overview; with reference to the highlighted pointers:
1. MINI-USB port. It allows to connect the UNO CONTROL UNIT to a PC via USB or to a USB stick, see
paragraph 5.5.
2. RS 232 port. It allows to connect the UNO CONTROL UNIT to a PC when the PC is at a distance
greater than 10 meters from the UNO CONTROL UNIT, see paragraph 5.6.
3. Power Input.
4. Base/lid. It is the power outlet for the Chamber’s Base and Lid, see paragraph 5.2.1.
5. Humidifier/Heated Tube. It is the power outlet for the Humidifier/Bubbling Column and the Heated
Tube, see paragraph 5.2.2.
6. Objective Heater. It is the power outlet for the Objective Heater, see paragraph 5.2.3.
7. Temperature Sensor port. It allows to connect the Temperature Sensor to UNO CONTROL UNIT,
see paragraph 5.4.
8. ON/OFF button.
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Figure 1. UNO CONTROL UNIT overview.
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4.1.1 BUNDLE 1) UNO-T. Overview and supplied equipment
The equipment that comes with UNO-T is composed by:
1. T-Sensor
2. UNO CONTROL UNIT
3. Y-CABLE-1 (see paragraph 5.2.1)
4. Objective Heater (optional see paragraph 5.2.3)
5. STAGE TOP CHAMBER (see paragraph 4.2)
6. Power adapter (voltage: 24V; max power consumption: 110W; max current: 4.6A)
7. MINI-USB-OTG (see paragraph 5.5)
Figure 2. UNO-T.
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4.1.2 BUNDLE 2) UNO-T-H-PREMIXED. Overview and supplied equipment
The equipment that comes with UNO-T-H-PREMIXED is composed by:
1. T-Sensor
2. UNO CONTROL UNIT
3. GF-REGULATOR (see paragraph 5.3.1 and 5.3.1.1)
4. TUBE-C (see Table 1)
5. Humidifier (see paragraph 5.2.2)
6. Heated Tube (see paragraph 5.2.2)
7. Objective Heater (optional, see paragraph 5.2.3)
8. STAGE TOP CHAMBER (see paragraph 4.2)
9. Y-CABLE-1 (see paragraph 5.2.1)
10. Y-CABLE-2 (see paragraph 5.2.2)
11. Power adapter (voltage: 24V; max power consumption: 110W; max current: 4.6A)
12. MINI-USB-OTG (see paragraph 5.5)
Figure 3. UNO-T-H-PREMIXED.
Description Photo
TUBE-C (X1)
Composed tube 300 cm long: Transparent PU O.D. 6mm
I.D. 4mm, Length 200 cm + Plastic Connector Promepla
4,8-3,2. + Sil O.D. 7mm I.D. 4mm, Length 50 cm + PTFE
0.2 micron + Sil O.D. 7mm I.D. 4mm, Length 50 cm
Table 1. Tubes for UNO-T-H-PREMIXED.
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4.1.3 BUNDLE 3) UNO-T-H-CO2. Overview and supplied equipment
The equipment that comes with UNO-T-H-CO2 is composed by:
1. OKO-AP (Air Pump) (see paragraph 5.3.2 and 5.3.2.1)
2. TUBE-A (see Table 2)
3. Air/CO2 Mixer (Gas Manual Mixer to mix Air and CO2) (see paragraph 5.3.2 and 5.3.2.1)
4. Pressure Gauge (see paragraph 5.3.2)
5. TUBE-A (see Table 2)
6. TUBE-C (see Table 2)
7. Glass Bottle – Humidifying Module (attention: FRAGILE, see paragraph 5.2.2)
8. Heated Tube (see paragraph 5.2.2)
9. Objective Heater (optional see paragraph 5.2.3)
10. STAGE TOP CHAMBER (see paragraph 4.2)
11. Y-CABLE-1 (see paragraph 5.2.1)
12. Y-CABLE-2 (see paragraph 5.2.2)
13. Power adapter (voltage: 24V; max power consumption: 110W; max current: 4.6A)
14. UNO CONTROL UNIT
15. MINI-USB-OTG (see paragraph 5.5)
16. T-Sensor
Figure 4. UNO-T-H-CO2.
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Description Photo
Tube-A (X2)
Single Tube 300 cm long: Transparent PU O.D. 6 mm I.D.
4mm, Length 300 cm.
TUBE-C (X1)
Composed tube 300 cm long: Transparent PU O.D. 6mm
I.D. 4mm, Length 200 cm + Plastic Connector Promepla
4,8-3,2. + Sil O.D. 7mm I.D. 4mm, Length 50 cm + PTFE
0.2 micron + Sil O.D. 7mm I.D. 4mm, Length 50 cm
Table 2. Tubes for UNO-T-H-CO2.
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STAGE TOP CHAMBER
For the equipment description of the Stage Top Chamber, refer to the dedicated attached Stage Top
Chamber manual.
5 INSTALLATION GUIDE
Assembly and Gas connection of the Bubbling Column
The bubbling column comes with UNO-T-H-PREMIXED and UNO-T-H-CO2. Figure 5 illustrates the bubbling
column components.
Figure 5. Bubbling Column components.
Follow the instructions below to correctly connect the gas tubes to the Bubbling Column (see Figure 6):
1. Plug the silicon end of TUBE-C to the Bubbling Column inlet (metal plug).
2. Push the Heated Tube from the PU end (yellow tube) all the way in to the push-to-fit Bubbling
column output.
3. Secure the Heated Tube silicon end to the chamber, making sure the tube is all the way in.
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Figure 6. Gas connections of the bubbling column.
Connections of the heating devices
This section describes how to connect the heating devices to UNO CONTROL UNIT.
5.2.1 Connection of the Chamber and of the Lid of the Incubating Chamber
Follow the instructions below to connect the Chamber and the Lid of the Incubating Chamber to UNO
CONTROL UNIT (see Figure 7):
1. Connect the Y-CABLE-1 to power output of Base and Lid on the back panel of UNO CONTROL UNIT.
The power output for the Chamber and for the Lid is the one on the left, labeled Base/Lid;
2. Connect the Chamber electric cable to its connector on the Y-CABLE-1 (Chamber color label is red);
3. Connect the Lid electric cable to its connector on the Y-CABLE-1 (Lid color label is yellow).
Figure 7. Chamber and Lid of the Incubating Chamber connection.
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5.2.2 Connection of the Humidifier and of the Heated Tube
Follow the instructions below to connect the Humidifier and the Heated Tube of the Incubating Chamber to
UNO CONTROL UNIT (see Figure 8):
1. Connect the Y-CABLE-2 to power output of Humidifier and Heated Tube on the back panel of UNO
CONTROL UNIT. The power output for the Humidifier and for the Heated Tube is the one in the
middle, labeled Humidifier/Heated Tube;
2. Connect the Humidifier cable to its connector on the Y-CABLE-2 (Humidifier color label is green);
3. Connect the Heated Tube cable to its connector on the Y-CABLE-2 (Heated Tube color label is gray).
Figure 8. Humidifier and Heated Tube connection.
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5.2.3 Connection of the Objective Heater (optional)
Connect the Objective Heater on the back panel of UNO CONTROL UNIT. The power output for the Objective
Heater is the one on the right, labeled Objective Heater (see Figure 9).
Figure 9. Objective Heater connection.
The cables connecting heating devices are not only color coded, but there is also a label
glued to their end.
Connection of the gas tubes
5.3.1 Gas connection for UNO-T-H-PREMIXED
UNO-T-H-PREMIXED works for application with pre-mixed gas. To make the gas flow into the chamber the
steps are the following:
1. Connect your Premixed Gas tank to the supplied GF Regulator inlet (During this operation make sure
that both the tank and the GF Regulator are closed).
2. Connect the GF Regulator output to the Humidifier bottle using TUBE-C.
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Figure 10. UNO-T-H-PREMIXED gas connection.
5.3.1.1 How to use the GF Regulator
The GF regulator allows to deliver the exact amount of gas flow, in fact since it is provided with a calibrated
orifice, it is possible to control the pressure in order to have the desired gas flow. Table 3 shows the relation
between Pressure and Flow for the GF Regulator.
Pressure [barg] Flow [l/min]
0,2 0,07
0,4 0,10
0,6 0,12
0,8 0,14
1,0 0,17
1,2 0,18
1,4 0,20
1,6 0,22
1,8 0,23
2,0 0,25
2,2 0,27
2,4 0,29
2,6 0,31
2,8 0,32
3,0 0,34
3,2 0,36
3,4 0,38
3,6 0,39
3,8 0,4 Table 3. UNO-T-H-PREMIXED Pressure/Flow relation.
Example: the desired gas flow is 0,2, hence the GF Regulator has to be set to 1,4 barg.
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5.3.2 Gas Connection for UNO-T-H-CO2
UNO-T-H-CO2 includes the Air/CO2 mixer and OKO-AP, it works for application with 100% CO2 and
background Air. To make the gas flow into the chamber the steps are the following:
1. Connect your CO2 Gas line/tank to the supplied Pressure Gauge inlet (During this operation make
sure that both the tank and the GF Regulator are closed). The CO2 tank Pressure Gauge has to be
set at 2 bar.
2. Connect the Pressure gauge to the “CO2 In”, which is located on the left of the rear panel of the
Air/CO2 Mixer using TUBE-A.
3. Connect OKO-AP to the “Air In”, which is located on the right of the rear panel of the Air/CO2 Mixer
using TUBE-A.
4. Connect the “Air+CO2 OUT”, located on the rear panel of the Air/CO2 Mixer, to the Humidifier
bottle using TUBE-C.
Figure 11. UNO-T-H-CO2 gas connection.
5.3.2.1 How to use the Air/CO2 Mixer
The Air/CO2 Mixer allows to generate CO2-Air mixtures with an adjustable CO2 concentration in the range
0-15%. To obtain the desired CO2 concentration regulate the air and CO2 flow rate using the two floating
ball flow meters. Air and CO2 flows can be regulated in the range 0.1 – 1 Nl/min and 0.013 – 0.13 Nl/min,
respectively. Refer to Air/CO2 Mixer user manual to set the proper air and CO2 flow rate in order to obtain
the desired CO2 percentage.
Tip Open the gas valves about 15 minutes before inserting the sample in the Micro Environmental
Chamber. Please follow carefully scheme in Figure 11.
Do not use the flow meters as closing-opening valves. Once regulated the CO2 flow pressure
(at P= 1bar, see Figure 11), use the flow meters carefully only for fine regulations.
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Table 4 reports a set of flow rates to obtain a concentration of CO2 equal to 5%. In Table 4, Target Gas
Percentage indicates the stream gas percentage that the operator wants during the experiment. Gas flow
rate values to set, in Normal litre per minute [NL/min], are the values of gas flow rate to set on the
graduated scale (see Figure 12). Total indicates the measured total gas stream flow rate.
Target Gas Percentage [%]
Gas flow rate values to set [Nl/min]
Air CO2 Air CO2 Total
95.00 5.00 0.60 0.03 0.63
95.00 5.00 0.80 0.04 0.84
95.00 5.00 1.00 0.05 1.05 Table 4. Flow meter regulation, two flow meters control unit. [Nl = Normal litre]
Tip If you need, ask Okolab for personalized Gas Percentage Tables
Example: the target Air percentage is 95.00%, the target CO2 percentage is 5.00%. In Table 4 three
possibilities that realize the required gas percentage are. Notice that the values are a compromise between
CO2 consumption, condensation (normally it decreases if Total gas stream flow rate increases) and medium
evaporation (normally it decreases if Total gas stream flow rate decreases). We could consider the second
line in Table 4. So, we regulate the two flow meters to obtain the desired relative quantity of air and CO2,
as shown in Figure 12. Notice that the correct position of the indicator (ball) is about in its middle.
Figure 12. Flow meters graduated scales, example for two gas lines.
Note If you use a single reservoir of air+5% CO2 the total gas steam flow rate suggested is 0.1 L/min.
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Connection of the Temperature Sensor
Connect the Temperature Sensor to the real panel, precisely to the green housing on the right, as indicated
in Figure 13.
Figure 13. Temperature Sensor connection.
Tip The Temperature Sensor has to be connected with “+” on top.
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MINI-USB port
The MINI-USB port allows the UNO CONTROL UNIT to be connected with the outside world.
5.5.1 Connection of a USB drive
Use MINI-USB-OTG cable to connect a USB Drive (not supplied) to the UNO CONTROL UNIT, as shown in Figure
14.
Figure 14. USB drive connection.
5.5.2 PC Connection via USB
Use a standard MINI-USB cable to connect UNO CONTROL UNIT to a PC, as shown in Figure 15. You can
communicate with UNO CONTROL UNIT via PC by using:
• DATA-LOG software. The software allows to log and analyze the data provided by UNO CONTROL
UNIT via PC. Refer to DATA-LOG user manual for more info.
• SDK (OKOLAB API) for integration in third party software http://www.oko-
lab.com/support#soft_int_tools.
Figure 15. PC connection via USB.
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RS232 port – PC connection via RS232
Use a standard RS232 cable to connect UNO CONTROL UNIT to a PC as shown in Figure 16. This option is
advisable when the PC is at a distance greater than 10 meters from the UNO CONTROL UNIT.
You can interface with UNO CONTROL UNIT via PC by using:
• DATA-LOG software. The software allows to log and analyze the data provided by UNO CONTROL
UNIT via PC. Refer to DATA-LOG user manual for more info.
• SDK (OKOLAB API) for integration in third party software http://www.oko-
lab.com/support#soft_int_tools.
Figure 16. PC connection via RS232.
Without a serial port COM, to install the Serial Communication cable to your PC, you need to
install a USB to serial converter following the manufacturer instructions. If you want to log,
you have to use the COM port number that Windows assigned to the drive.
Installation of the Stage Top Chamber
For the installation of the Stage Top Chamber refer to the dedicated attached Stage Top Chamber manual.
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6 USER INTERFACE
This chapter describes the user interface of the UNO CONTROL UNIT.
Homepage
Figure 17 shows the main control panel, in particular it highlights 19 pointers detailed below.
Figure 17. Home page.
1. System Information. Press here to know generic info about UNO and its running time. See paragraph
6.2.
2. Home. Press here to return to the home page.
3. Settings. Press here to access system options and settings. See paragraph 6.3.
4. Status. Press here to see the status of your system (you will need this page in case of assistance).
See paragraph 6.4.
5. Glance mode. Press here to switch the display to glance mode. See paragraph 6.3.8.5 and 6.5.
6. UNO status led. See paragraph 6.1.1 for the color mapping.
7. Chamber (or T Sensor) current temperature.
8. Temperature set point.
9. Minimum and Maximum temperature values in the actual time window.
10. Temperature at the heating tube.
11. Temperature at the T sensor.
12. Temperature at the lid.
13. Temperature at the base.
14. Temperature at the Objective heater.
15. Support. Press here to learn how to contact OKOLAB. See paragraph 8.1.
16. Temperature at the Bubbling Column.
17. Open Incubator.
18. PC or USB Connection indicator.
19. Logging icon.
20. Temperature evolution graph.
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6.1.1 Colors meaning
The GREEN indicator indicates that the set-point value has been reached (within the
tolerance you have set in the alarm subpage, see paragraph 6.3.7) and that the system is
working properly.
The YELLOW indicator means that the system is working towards reaching the set-points
(transient state). No actions on your part are required. Please note the Yellow light will
also appear every time you change temperature set point.
The ORANGE indicator means that the current temperature is out of the tolerance you have
set (see paragraph 6.3.7). Verify that all cables are properly connected. Check all the
enclosures to verify that they are properly closed.
The RED indicator means that there is a problem with the unit itself (for example the
Temperature sensor has been damaged). Turn the system off, wait 5 minutes, and turn it
back on. If the problem continues, contact Okolab at www.oko-lab.com
6.1.2 Changing the set point temperature
To input a new set-point temperature for a heating device, touch the corresponding tab, indicated as Pointer
1 in Figure 18(a).
The set point regulation page will appear as in the Figure 18(b). You can modify the set point by clicking on
+/-, see Pointer 2. Once you have input the new set point temperature, press Set to save (or Cancel to
undo), see Pointer 3.
Tip After any change in the set point value UNO CONTROL UNIT enters into a transient regime, the Status
Indicator and the Top LED turn yellow (see paragraph 6.1.1). During the transient regime, U
will not trigger on alarm. The maximum length of the transient regime is set by the operator as indicated in
paragraph 6.3.7.
(a)
(b)
Figure 18. Changing the set point temperature.
6.1.3 Graph view
By pressing on the Temperature evolution chart (pointer 1 in Figure 19(a)), the Graph view page will open.
By checking or unchecking the checkbox Grid (pointer 2 in Figure 19(b)), it is possible to see or to remove,
respectively, the grid behind the graph.
27
(a)
(b)
Figure 19. Graph view.
System Information
Press the icon to access the Info Page, as shown in Figure 20. This page contains the information
related to UNO CONTROL UNIT version.
Figure 20. Info page selection
Tip Please have this information handy when contacting Okolab for support.
Settings
Press on Settings icon to enter the Settings menu, as shown in Figure 21.
Figure 21.How to access the Settings page.
28
The settings menu has 11 items, as Figure 22(a) and Figure 22(b) show:
1. Control Mode. See paragraph 6.3.1.
2. Chamber & Insert. See paragraph 6.3.2.
3. Obj. Heater. See paragraph 6.3.3.
4. Humidity. See paragraph 6.3.4.
5. Calibrations. See paragraph 6.3.5
6. Logging. See paragraph 6.3.6.
7. Alarms. See paragraph 6.3.7.
8. Display. See paragraph 6.3.8.
9. Password. See paragraph 6.3.9.
10. Summary. See paragraph 6.3.10.
11. Auto on/off. See paragraph 6.3.11.
(a)
(b)
Figure 22. Graph view.
Each of the aforementioned item will be detailed in the following.
6.3.1 Control Mode
Press on to enter the Control Mode menu, as Figure 23 shows.
Figure 23. Control Mode menu.
Once you are in the Control Mode submenu it is possible to choose:
1. Sample Control mode. Figure 24 shows how to select Sample Control mode:
• Press on the Sample icon;
• Press Save.
29
Now the UNO CONTROL UNIT will start operating in Sample Control Mode.
Figure 24. Sample Control mode.
Note Sample Control mode allows you to take the temperature feedback from the specimen with the
dedicated Temperature Sensor. This mode gives the opportunity to control the environment directly inside
the plate with the drawback of having a plate dedicated to the temperature acquisition.
2. Chamber Control mode. Figure 25 shows how to select Chamber Control mode:
• Press on the Chamber icon;
• Press Save.
Figure 25. Chamber Control mode.
Note Chamber Control mode allows you to take the temperature feedback directly from the chamber
temperature sensor, without the need of having floating wires for a dedicated specimen’s Temperature
Sensor.
At this point the page Chamber Offset will pop out, see Figure 26. In fact, there are two options for operating
in Chamber Control mode:
1. Factory: by pressing on the Factory button the UNO CONTROL UNIT will use the calibration
parameters already optimized by Okolab.
2. Calibrate: by pressing on the Calibrate button the UNO CONTROL UNIT will start a routine to
calibrate its parameters with the help of the external Temperature Sensor, to be placed inside the
chamber before the operation. Figure 27(a) shows the UNO CONTROL UNIT during the calibration
procedure; by clicking Options, the menu Advanced calibration parameters will appear and, here, it is
possible to set the calibration Accuracy and Precision, see Figure 27(b).
30
Figure 26. Chamber Control mode – Chamber Offset.
(a)
(b)
Figure 27. Chamber Control mode – Self Calibration.
6.3.1.1 Open Incubator
During the normal operation it could be necessary to open the incubator. Figure 28 shows the procedure to
follow when opening incubator:
1. Press on Open Incubator, before removing the lid, as Figure 28 (a) shows. Now you can open the
incubator;
2. Once you are done close the chamber and wait for 2 minutes in order to let the environment achieve
steady state; press on Incubator Closed, as Figure 28 (b) shows, and the UNO CONTROL UNIT will
resume operating normally.
(a)
(b)
Figure 28. Control mode – Open Incubator.
31
Opening the incubator can lead to controller instability since both the specimen and the
inside of the chamber are exposed to the external environment. In order to avoid the
problem, UNO CONTROL UNIT has to stay in a holding state until the incubator is closed and
its environment is in a steady state. This can be achieved using the Open Incubator
procedure.
6.3.2 Chamber & Insert
UNO CONTROL UNIT is available for a plethora of chambers to perfectly fit your specific microscope setup
and the plates you need to use. Since the bottom of the chamber is in contact with the external
environment, the UNO CONTROL UNIT has to modify its controlling parameters in dependence of the specific
couple Chamber/Insert.
To setup your Chamber/Insert configuration press on Chamber & Insert, as Figure 29(a) shows. At this
point the Chamber & Insert submenu in Figure 29(b) will appear.
(a)
(b)
Figure 29. Chamber & Insert.
Figure 30(a) and (b) show the selection menus for Chamber and Insert, respectively. You can navigate
through the menus to choose the objects present in your UNO CONTROL UNIT configuration.
(a)
(b)
Figure 30. Chamber & Insert configuration. (a)Chamber selection; (b) Insert selection.
32
6.3.2.1 Compatible chambers
Table 5 reports all the available chambers for UNO STAGE TOP INCUBATOR.
Chambers: H301-K-FRAME H301-NIKON-TI-S-ER H301-OLYMPUS-IX3-SVR H301-PRIOR-H117 Detail: Fits standard XY stages with k-frame insert (160x110mm).
Chambers: H301-LUDL96A602 H301-MCL-Z100/500 H301-NIKON-NZ100/200/500-N H301-PI-736-160x110 Detail: Fits Z-piezo stages models listed.
Chambers: H301-MINI Detail: Fits in any MW plate holder.
Chambers: H301-NIKON-TI-SR H301-OLYMPUS-IX-SUSP Detail: Fits rectangular stages with circular opening.
Chambers: H301-EC-LG-BL Detail: Fits Leica Super Z Galvo Stage (SP5/SP8).
Chambers: H301-PRIOR-NZ500 Detail: Fits Prior Z-piezo stages models NZ250/400/500.
33
Chambers: H301-PRIOR-NZ100-H107 H301-PRIOR-NZ100-H117 H301-PI-736-ZR15/ZR25 Detail: Fits Prior Z-piezo stages models listed.
Chambers: H301-UP Detail: Fits any Upright Microscopes. It allows the usage of dipping lenses.
Chambers: H301-EC-LG-UP-BL Detail: Fits Upright Leica Super Z Galvo Stage. It allows the usage of dipping lenses.
Chambers: H301-DUAL-GAS Detail: Chamber with two independent gas compartments.
Chambers: CUSTOM Detail: Parameters for custom chambers.
Table 5. Compatible chambers.
34
6.3.3 Objective Heater
Press on Objective Heater icon to enter the Objective Heater menu, see Figure 31(a).
The Objective Heater is disabled by default, to activate it press on the Enabled icon, see Figure 31(b). At
this point you can choose:
1. Ramp Rate. By pressing on this button the submenu in Figure 32 will appear; here you can tune the
ramp rate for the temperature of the Objective Heater.
2. Cancel. To Cancel your choice.
3. OK. To confirm your choice; moreover, after pressing this button, the menu in Figure 33 will appear
giving you further options.
(a)
(b)
Figure 31. Objective Heater. (a) How to access the Objective Heater menu; (b) Objective Heater menu.
Figure 32. Ramp rate.
Before enabling the Objective Heater, make sure that it is correctly mounted around your
objective and that it is connected to the UNO CONTROL UNIT.
Figure 33 shows the menu that appears when selecting the Objective Heater feature, with reference to the
Pointers 4 options are available:
1. Log: by pressing this button you can recall parameters obtained with previously performed
calibrations, see Figure 34(a);
2. Factory: by pressing this button UNO CONTROL UNIT will resume the factory offset;
3. Manual: by pressing this button can find the best offset value manually, step by step, see Figure
34(b);
4. Calibrate: by pressing this button UNO CONTROL UNIT will perform a calibration routine for your
specific environment, see paragraph 6.3.5.
35
Figure 33. Objective Heater Enabled.
(a)
(b)
Figure 34. Objective Heater options. (a) Log; (b) Manual.
6.3.4 Humidity
Press on Humidity icon to enter the Humidity menu, see Figure 35.
Figure 35. How to access the Humidity menu.
Figure 36(a) shows the Humidity menu. Once the Humidity control enabled checkbox is checked, UNO
CONTROL UNIT will perform humidity control acting on the temperatures of both the humidifier’s bottle
and the heated tube.
In order to make the humidity control work properly, the user has to set the actual room temperature, see
Figure 36(a).
UNO CONTROL UNIT, by default, supplies a humidity level of 90%; whereas when you require a different
humidity level, it is possible to act on the Offset temperature, see Figure 36(b): rising this value will lead
to rising the humidity level as well.
36
(a)
(b)
Figure 36. Humidity menu. (a) Main menu; (b) Offset.
6.3.5 Calibration
Press on Calibration icon to enter the Settings menu, as shown in Figure 37.
Figure 37. How to access Calibration menu.
Once you are in the Control Mode submenu it is possible to choose:
1. Chamber Calib. Here you can perform the calibration of the chamber, see paragraph 6.3.5.1.
2. T Sensor. Here you can perform the calibration of the Temperature Sensor, see paragraph 6.3.5.2.
3. Obj calibration. Here you can perform the calibration of the Objective Heater, see paragraph
6.3.5.3.
6.3.5.1 Chamber Calibration
Press on Chamber Calib icon , as Figure 38 shows, to enter the Chamber Calibration submenu in Figure
39.
Figure 38. Chamber calibration.
37
Two options are available to the Chamber Calibration, see Figure 39:
1. Factory: by pressing on the Factory button the UNO CONTROL UNIT will use the calibration
parameters already optimized by Okolab.
2. Calibrate: by pressing on the Calibrate button the UNO CONTROL UNIT will start a routine to
calibrate its parameters with the help of the external Temperature Sensor, to be placed inside
the chamber before the operation. Figure 40(a) shows the UNO CONTROL UNIT during the
calibration procedure; by clicking Options, the menu Advanced calibration parameters will
appear and, here, it is possible to set the calibration Accuracy and Precision, see Figure 40(b).
Figure 39. Chamber Control mode – Chamber Offset.
(a)
(b)
Figure 40. Chamber Control mode – Self Calibration.
6.3.5.2 T sensor Calibration
The Temperature Sensor of UNO CONTROL UNIT is calibrated in Okolab at three different temperatures,
i.e. 35.0, 37.0 and 40.0°C. To view the factory calibration data, press on the T sensor calibration icon
as shown in Figure 41(a) and then on the tab labeled Factory as shown in Figure 41(b); the page with the
calibration data will open, as shown in Figure 42.
38
(a)
(b)
Figure 41. Calibration data. (a) T sensor calibration page selection; (b) T sensor calibration page.
Figure 42. Free sensor factory offset
To re-calibrate the T sensor, follow the instructions below.
1. Set a water bath to the temperature you use as set point for the heating devices during standard
operation (most of the times it is 37°C);
2. Measure water temperature by immersing the Temperature Sensor and a Certified Thermometer;
3. Type in the reading of the certified thermometer by pressing on the pencil , as shown in Figure
43(a);
4. Press on Set to adjust to reading of the Temperature Sensor against the reading of the certified
thermometer, as shown in Figure 43(b).
(a)
(b)
Figure 43. How to re-calibrate the T sensor
Tip Press on icon to receive instructions on how to calibrate the T-Sensor.
Tip Okolab recommends using a certified thermometer as external reference.
39
6.3.5.3 Objective Heater Calibration
Press on Obj calibration icon , as Figure 38 shows, to enter the Objective Heater Calibration.
Figure 44. Objective Heater calibration.
To perform the calibration, refer to the following steps:
1. Stick the Temperature Sensor (green wire) at the bottom of the dish under observation;
2. Fill the dish with distilled water until the Temperature Sensor is completely immersed in water;
3. Close both this dish and the incubating chamber with their own respective lids;
4. Touch the CALIBRATE button (pointer 4 in Figure 33);
5. The warning dialog in Figure 45(a) will appear, after double checking the previous steps, click OK
and the calibration will start as Figure 45(b) shows.
Note 1. To calibrate the Objective Heater be sure that Chamber calibration has already been
performed.
(a)
(b)
Figure 45. Objective Heater calibration.
Figure 46 shows the Advanced calibration parameters for the procedure, that are accessible by pressing on
Options during the calibration procedure.
40
Figure 46. Objective Heater calibration. Advanced calibration parameters.
6.3.6 Logging
UNO CONTROL UNIT is equipped with local memory to store the temperature and calibration data on its
internal memory. These data can be then downloaded on a USB stick at user request. In order to activate
the logging, follow the steps indicated below:
1. Press on Logging icon and flag Internal memory in the page that appears, see Figure 47.
Tip When activating the logging on the internal memory, you can access the Logging page also by pressing
the activated logging icon on the Homepage, as shown in Figure 48.
(a)
(b)
Figure 47. Logging. (a) How to enter in the logging menu; (b) logging in internal memory
Figure 48. How to access the Logging page from the Homepage
2. Press on the icon to insert the logging time, see Figure 49. You can change this time in the range
1-60 seconds.
41
(a)
(b)
Figure 49. How to set the logging time
3. Choose how you want to split the data when downloaded, by pressing on Day, Week or Month, see
Figure 49 (a).
Tip If you select Day, the data will be split in files, each one containing the data on one day. If you select
Week, the data will be split in files, each one containing the data on one week.
4. Write the file name suffix to be reported in the downloaded files by pressing on the keyboard icon
, see Figure 50(a). Then press on Save, see Figure 50(b).
Tip With the selections done in Figure 49 and in Figure 50, the data will be downloaded in the USB drive
as shown in Figure 51.
(a)
(b)
Figure 50. Data Logging. How to define the suffix for the downloaded files
Figure 51. Downloaded files on the pen drive
Note If you press on the screwdriver , see Figure 52(a) you can view the logging starting date, the
available memory and the latest download, see Figure 52(b).
Note The available memory depends on the time interval you have inserted in the Logging page. The
default logging time is 30 seconds.
42
(a)
(b)
Figure 52. Internal memory status. (a) Logging page settings; (b) Internal log page settings
To download the data, press on To USB or on Erase if you want to delete them, see Figure 52(b).
(a)
(b)
Figure 53. Download to USB
You can activate the logging also on the USB stick, by flagging on USB pen drive, see Figure 54(a).
(a)
(b)
Figure 54. Logging on USB drive. (a) USB drive selection; (b) Reminder to connect the USB drive
In this case, UNO advices you that a pen drive must be connected to the USB port, see Figure 54(b), and
will store the data on the USB drive.
6.3.7 Alarms
When UNO CONTROL UNIT triggers on alarm, it may activate both visual and audible alarms.
To set the alarms specifications, press on the Alarms icon ; see Figure 55(a) then follow the indications
below:
1. Insert the alarm time; i.e. the time for which the temperature of the Heating Devices may remain
outside the allowed tolerance before the controller triggers in alarm, see 1 in Figure 55(b)
43
Note The alarm time range is 5-20 minutes.
2. Insert the Tolerance value, see 2 in Figure 55(b). This value defines the allowed tolerance form the set
point.
Note The tolerance value range is 0.1-2.0 °C
3. Insert the transient time; i.e. the maximum time that the system can take to reach a new set point,
without triggering the alarm, see 3 in Figure 55(b).
(a)
(b)
Figure 55. Alarms settings. (a) How to enter the Alarm setting menu; (b) Alarm configuration
Note The transient time range is 30-240 minutes.
Tip After any change in the set point value the device enters into a transient regime.
4. Flag the tab labeled Buzzer if you want the alarm to be acoustical, additionally to being visual, see
Figure 55(b)
5. Flag the tab labeled Enabled to enable the Alarms logic, see Figure 55(b)
6. To disable the visual and acoustical alarms, remove the flag from the tab labeled Enabled. In this case
the Alarms page becomes as shown in Figure 56.
Figure 56. Alarms page when the alarms are disabled
Tip When disabling the alarms, UNO CONTROL UNIT goes into alarm condition only when the status LED
becomes red (for example is a device is accidentally disconnected).
Note By Pressing on Log (see Figure 57(a)), you can view all the times that the status LED of the two devices
have changed color (see Figure 57(b)).
44
(a)
(b)
Figure 57. Logging alarms page
Press on To USB if you want to download the alarms data to a USB drive or on Erase if you want to delete
them.
6.3.8 Display
To enter in the Display configuration menu, press on the icon , as shown in Figure 58(a) and the Display
page shown in Figure 58(b) will appear.
1. Options. See paragraph 6.3.8.1.
2. Brightness. See paragraph 6.3.8.2.
3. Calibration. See paragraph 6.3.8.3.
4. Date & Time. See paragraph 6.3.8.4.
5. Visual Effects. See paragraph 6.3.8.5.
6. Factory Reset. See paragraph 6.3.8.6.
(a) (b)
Figure 58. Display settings page. (a) How to enter the Display menu. (b) Display menu.
6.3.8.1 Options
The Display Options menu allows t set the time frame in which the minimum and maximum temperature
values are collected. To insert the time frame, press on the icon and slide your finger along the
Displayed time frame bar or click the +/- icons, then press Save, see Figure 59.
45
(a)
(b)
Figure 59. Options subpage configuration. (a) Select options; (b) Setting display options
Note The max and minimum temperature value will be displayed in the top left box in the Homepage (see
pointer 9 in Figure 17).
To change the touch sound frequency press and slide your finger along the setting bar indicated with Sound
frequency, then press Save, see Figure 59(b)
Note To activate a sound at each touch, flag the tab labeled Touch Sound
6.3.8.2 Brightness
To modify display Brightness press the icon and scroll the bar or press the +/- icons in the page that
opens, as shown in Figure 60.
(a)
(b)
Figure 60. Brightness display page
Set the required configuration and then press on Save to obtain the right configuration of Brightness, see
Figure 60(b).
6.3.8.3 Calibration
When pressing the calibration icon , the procedure for the calibration of the touch screen will start.
46
Figure 61. Touch screen calibration.
If the touch screen is not calibrated and it is not possible to navigate in the menu, keep pressed the ON/OFF
button on UNO-CONTROL-UNIT for 7 seconds to start the touch screen calibration (see Figure 62). While
holding the button, the pop-up message shown in Figure 63 a appears. Then tap blue calibration dots until
the calibration is complete (see Figure 63 b).
Figure 62. How to enter in touch screen Calibration.
47
Figure 63. Start touch screen calibration (a – b).
6.3.8.4 Date & Time
To set Date & Time, follow the instructions below:
1. Press the Date & Time icon , see Figure 64(a)
2. Set the values, as shown in Figure 64(b)
3. Click on Save to save or Cancel to undo
(a)
(b)
Figure 64. Select Date&Time configuration.
Tip Flag the 24 hours box, if you want to use the hour format based on 24 hours.
6.3.8.5 Visual Effects
To enter in the Display visual effects menu, press on the icon , see Figure 65 (a).
(a)
(b)
Figure 65. Display Visual Effects. (a) How to enter in Visual Effects menu; (b) Visual Effects menu.
48
The Top LED settings allows you to when the Top LED should be illuminated: if you select Never, the Top
LED will never illuminate, if you select On Alarm, the Top LED will illuminate only when the UNO CONTROL
UNIT is in Alarm Status (see paragraph 6.1.1), if you select Always, the Top LED will always illuminate.
Glance Mode Setting (see also paragraph 6.5)
The Glance mode setting defines the color of the Top LED and of the digits displayed in Glance Mode. If you
select White, the Top LED illuminates in white color (according to the criterion selected in Top LED setting)
and the digits displayed in Glance Mode are white. If you select Dynamic the color of the Top LED and the
color of the digits in Glance Mode follow the color coding reported in paragraph 6.1.1.
Tip To swap between Icon and Glance mode press on the icon .
6.3.8.6 Factory Reset
To reset the display settings, press on the icon , see Figure 66(a). At this point Figure 66(b) will appear,
asking to confirm the Factory Reset of the display.
(a)
(b)
Figure 66. Factory reset.
6.3.9 Password
With the UNO CONTROL UNIT, as well as with many Okolab’s products, the user have the chance to secure
his laboratory setups by using a password. The password, when enabled, will be asked, in the following
procedures:
• Heating devices calibration procedures
• T sensor calibration
• Data download
• Data erase
To enable the password protection, follow the steps below:
1. Press on the Password icon , see Figure 67(a)
2. Flag the tab Password enabled as shown in Figure 67(b)
49
(a)
(b)
Figure 67. Password. (a) Password settings menu; (b)password subpage
Note The default password is okolab, and it is disabled. Okolab recommends changing this password.
Note If you forgot the password, use the restore password reported on the certificate attached to UNO
CONTROL UNIT.
3. To change the password, press on the icon and follow the indications that will appear.
6.3.10 Summary
The Summary Page reports technical information. To enter the Summary menu, press on the settings icon
, as shown in Figure 68(a), then press on Summary icon . The page reported in Figure 68(b) will
appear.
Note This page is useful essentially for technical/control reasons. During a support case the engineers of
Okolab’ s team may ask you to read some of the data reported in Summary page
(a)
(b)
Figure 68. Auto on/off. (a) Auto on/off menu; (b) Auto on/off subpage.
6.3.11 Auto on/off
Press on to enter the Auto on/off menu, see Figure 69(a). Once you are in the Auto on/off menu, you
can choose between, see Figure 69(b):
• Auto turn on: to schedule the automatic turn on of UNO CONTROL UNIT;
50
• Auto turn on: to schedule the automatic turn on of UNO CONTROL UNIT.
(a)
(b)
Figure 69. Auto on/off. (a) Auto on/off menu; (b) Auto on/off subpage.
We will now describe the available options for the Auto turn on procedure only, but the description perfectly
fits also to the Auto turn off procedure, whose point to point description has been omitted for the sake of
simplicity.
Figure 70(a) shows how to access the Auto turn on subpage. As Figure 70(b) depicts, by default the Auto
turn on/off is disabled.
(a)
(b)
Figure 70. Auto on/off options.
It is possible to set a time when UNO CONTROL UNIT has to start Every day by pressing on its tab, or it is
possible to select the specific days in the week by choosing Specific days, see Figure 71(a).
Pressing Save will make your choice effective.
(a)
(b)
Figure 71. Auto on/off on specific days.
51
Status
On this page you can read the Status of UNO CONTROL UNIT; this is useful to check that the system is
working properly. Most of the parameters displayed are technical/control parameters that you may be asked
to access in case you have requested for Okolab support.
(a)
(b)
Figure 72. Status page.
Glance Mode
Pressing on ‘Glance Mode’ (see Figure 73(a)), you can change the data visualization format on the Homepage
that turns into a brief information about the state of the atmosphere inside the incubator, as Figure 73(b)
shows.
(a)
(b)
Figure 73. Glance Mode view.
This visualization type allows you to have a quick overview of the device from far away. See paragraph
6.3.8.5 for Glance Mode settings.
52
7 MAINTENANCE
To get a correct UNO CONTROL UNIT operation over time:
• Use a polishing cloth or dry cloth to wipe off dust and dirt.
• Never use thinners, benzene, solvents on or near the devices, since these could corrode the
surfaces.
• Verify the status of all hoses/tubing and replace those damaged.
• After 2 years, disconnect all polyurethane tubing, cut the last 1 cm of the tubing and reconnect
them to the connectors.
Before cleaning the unit, make sure it is unplugged form the power source.
Water must not enter the system.
53
8 SUPPORT
Support page
The support page can be reached pressing on the question mark in Homepage (see Figure 74(a)), the page
in Figure 74(b) will open. Here you can see the Italian and American Okolab contacts, moreover you can use
the QR code to get to the Okolab site.
(a)
(b)
Figure 74. Support page.
In order to send directly an email to the Okolab staff, you can use the QR codes shown in the following
images:
• For commercial info:
Figure 75. QR code for commercial info contact.
• For technical info:
Figure 76.QR code for technical info contact.
54
Support Contacts
Please, do not hesitate to contact Okolab if you need any further commercial information or technical
support.
Please, check Okolab web site www.oko-lab.com for news, events, new products and general FAQs.
For COMMERCIAL SUPPORT: [email protected]
Phone +39 081 806 2624
Fax: +39 081 876 4410
Mobile: +39 348 9680717
For TECHNICAL SUPPORT: [email protected]
Phone +39 081 806 3470
Mobile: +39 348 9680718
Okolab S.r.l.
Via A. Olivetti, 1 - 80078 Pozzuoli, NA
Italy
Web Conference For Assistance And Training
In case you requested on line support and/or web conference assistance for system assembling and
experiment support, you received a web cam. Please follow these guidelines to receive optimum service.
• Webcam installation (instruction and Cdrom included)
• Latest Skype® software installed (www.skype.com)
• Register yourself on www.skype.com to have an account (Skype_ID)
• Set the audio and video and test them using Skype.
• Contact our technical support ([email protected]) by e-mail to take an appointment for the web
assistance.
55
9 TECHNICAL SPECIFICATION
UNO CONTROL UNIT Technical Specifications
Operating Temperature +3°C above ambient ~+60°C
Storage Temperature -5°C ~ +60°C
Operating Humidity 90%
Power Consumption 24V- 5A, 120 W max
Weight 0,4 Kg
10 TROUBLESHOOTING
Incorrect operations are often mistaken for trouble and malfunctions. If you think that there is something
wrong with a component, check the points below. Sometime the trouble may lie in another component.
Investigate the others components, electrical appliance and pneumatic system being used. If the trouble
cannot be rectified even after exercising the checks listed below, ask for Okolab support at info@oko-
lab.com
Symptom Probable cause Remedy
Acoustic alarm sounds The current temperature values
are far temperature set-points
Check the cable connections and
if the problem is still present
contact Okolab Bubbling column is not showing
bubbles Not properly inserted tubes
Strongly push the tube into the
bubbling column inlet
Data download does not start
USB pendrive is not correctly
formatted
Make sure that USB pendrive is
FAT32 formatted
USB pendrive is damaged Format your USB pendrive using
FAT32 file system
Calibration/alarm events are not
logged
Internal memory is full (a
warning icon in homepage is
present)
Erase the full memory. If you
need this information, download
it before erasing the data
The device does not log the data
on the internal memory but only
on the USB pendrive
Internal memory is full (a
warning icon in homepage is
present)
Erase the full memory. If you
need this information, download
it before erasing the data
The screen does not respond
properly or not at all The display is not calibrated
Press and hold the power button
for more than 10 seconds and the
touch calibration will start
I checked the previous
troubleshooting but I cannot solve
the problem
57
11 FIGURE LIST
Figure 1. UNO CONTROL UNIT overview. .............................................................................. 10 Figure 2. UNO-T. ............................................................................................................ 11 Figure 3. UNO-T-H-PREMIXED. .......................................................................................... 12 Figure 4. UNO-T-H-CO2. ................................................................................................... 13 Figure 5. Bubbling Column components. ................................................................................ 15 Figure 6. Gas connections of the bubbling column. .................................................................... 16 Figure 7. Chamber and Lid of the Incubating Chamber connection. ................................................ 16 Figure 8. Humidifier and Heated Tube connection. .................................................................... 17 Figure 9. Objective Heater connection. .................................................................................. 18 Figure 10. UNO-T-H-PREMIXED gas connection. ..................................................................... 19 Figure 11. UNO-T-H-CO2 gas connection. .............................................................................. 20 Figure 12. Flow meters graduated scales, example for two gas lines. .............................................. 21 Figure 13. Temperature Sensor connection. ............................................................................ 22 Figure 14. USB drive connection. ......................................................................................... 23 Figure 15. PC connection via USB. ....................................................................................... 23 Figure 16. PC connection via RS232. .................................................................................... 24 Figure 17. Home page. ..................................................................................................... 25 Figure 18. Changing the set point temperature. ........................................................................ 26 Figure 19. Graph view. ...................................................................................................... 27 Figure 20. Info page selection ............................................................................................. 27 Figure 21.How to access the Settings page. ............................................................................ 27 Figure 22. Graph view. ...................................................................................................... 28 Figure 23. Control Mode menu. ........................................................................................... 28 Figure 24. Sample Control mode. ......................................................................................... 29 Figure 25. Chamber Control mode. ....................................................................................... 29 Figure 26. Chamber Control mode – Chamber Offset. ................................................................ 30 Figure 27. Chamber Control mode – Self Calibration. ................................................................. 30 Figure 28. Control mode – Open Incubator. ............................................................................. 30 Figure 29. Chamber & Insert. .............................................................................................. 31 Figure 30. Chamber & Insert configuration. (a)Chamber selection; (b) Insert selection. ......................... 31 Figure 31. Objective Heater. (a) How to access the Objective Heater menu; (b) Objective Heater menu. ... 34 Figure 32. Ramp rate. ....................................................................................................... 34 Figure 33. Objective Heater Enabled. .................................................................................... 35 Figure 34. Objective Heater options. (a) Log; (b) Manual. ............................................................ 35 Figure 35. How to access the Humidity menu. .......................................................................... 35 Figure 36. Humidity menu. (a) Main menu; (b) Offset. ................................................................. 36 Figure 37. How to access Calibration menu. ............................................................................ 36 Figure 38. Chamber calibration. ........................................................................................... 36 Figure 39. Chamber Control mode – Chamber Offset. ................................................................ 37 Figure 40. Chamber Control mode – Self Calibration. ................................................................. 37 Figure 41. Calibration data. (a) T sensor calibration page selection; (b) T sensor calibration page. ........... 38 Figure 42. Free sensor factory offset ..................................................................................... 38 Figure 43. How to re-calibrate the T sensor ............................................................................. 38 Figure 44. Objective Heater calibration. .................................................................................. 39 Figure 45. Objective Heater calibration. .................................................................................. 39 Figure 46. Objective Heater calibration. Advanced calibration parameters. ....................................... 40 Figure 47. Logging. (a) How to enter in the logging menu; (b) logging in internal memory ...................... 40 Figure 48. How to access the Logging page from the Homepage ................................................... 40 Figure 49. How to set the logging time ................................................................................... 41 Figure 50. Data Logging. How to define the suffix for the downloaded files ....................................... 41 Figure 51. Downloaded files on the pen drive ........................................................................... 41 Figure 52. Internal memory status. (a) Logging page settings; (b) Internal log page settings ................... 42 Figure 53. Download to USB ............................................................................................... 42 Figure 54. Logging on USB drive. (a) USB drive selection; (b) Reminder to connect the USB drive .......... 42 Figure 55. Alarms settings. (a) How to enter the Alarm setting menu; (b) Alarm configuration ................. 43 Figure 56. Alarms page when the alarms are disabled ................................................................ 43 Figure 57. Logging alarms page ........................................................................................... 44 Figure 58. Display settings page. (a) How to enter the Display menu. (b) Display menu. ....................... 44 Figure 59. Options subpage configuration. (a) Select options; (b) Setting display options ...................... 45
58
Figure 60. Brightness display page ....................................................................................... 45 Figure 61. Touch screen calibration. ..................................................................................... 46 Figure 62. How to enter in touch screen Calibration. .................................................................. 46 Figure 63. Start touch screen calibration (a – b). ....................................................................... 47 Figure 64. Select Date&Time configuration. ............................................................................. 47 Figure 65. Display Visual Effects. (a) How to enter in Visual Effects menu; (b) Visual Effects menu. ......... 47 Figure 66. Factory reset..................................................................................................... 48 Figure 67. Password. (a) Password settings menu; (b)password subpage ........................................ 49 Figure 68. Auto on/off. (a) Auto on/off menu; (b) Auto on/off subpage. ............................................. 49 Figure 69. Auto on/off. (a) Auto on/off menu; (b) Auto on/off subpage. ............................................. 50 Figure 70. Auto on/off options. ............................................................................................. 50 Figure 71. Auto on/off on specific days. .................................................................................. 50 Figure 72. Status page. ..................................................................................................... 51 Figure 73. Glance Mode view. ............................................................................................. 51 Figure 74. Support page. ................................................................................................... 53 Figure 75. QR code for commercial info contact. ....................................................................... 53 Figure 76.QR code for technical info contact. ........................................................................... 53
12 TABLE LIST
Table 1. Tubes for UNO-T-H-PREMIXED. ............................................................................... 12 Table 2. Tubes for UNO-T-H-CO2. ....................................................................................... 14 Table 3. UNO-T-H-PREMIXED Pressure/Flow relation. ............................................................... 19 Table 4. Flow meter regulation, two flow meters control unit. [Nl = Normal litre] .................................. 21 Table 5. Compatible chambers. ........................................................................................... 33
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13 MANUAL REVISION TABLE
Revision Number Additions or changes Date Editor
01 Edited May 2016 Francesco Marino
02 Changes in the connection
of the heating devices Nov 2016 Francesco Marino
03 Updating supplied
equipment Feb 2019 Martina Mauriello
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14 LIST OF SPARE PARTS
UNO-T-H-PREMIXED
Product Spare part type Spare part code Frequency of replacement
UNO-T-H-PREMIXED 4x 0.2 micron filter SP-FILTER-002 Every 3 months of usage
for each filter.
UNO-T-H-PREMIXED Tubes + Filter kit SP-UNO-THP-TUBES-KIT In case of damage
Humidifier Glass bubbling column SP-DRYER-BOTTLE In case of damage
UNO-T-H-CO2
Product Spare part type Spare part code Frequency of replacement
UNO-T-H-CO2 4x 0.2 micron filter SP-FILTER-002 Every 3 months of usage
for each filter.
UNO-T-H-CO2 Tubes + Filter kit SP-UNO-THCO2-TUBES-KIT In case of damage
Humidifier Glass bubbling column H301-BC In case of damage
61
WARRANTY Okolab S.r.l. warrants its products to be free of defects in materials and workmanship for a period of one
year starting from invoice date. If the unit malfunctions, it must be returned to the factory for evaluation.
If the equipment has to be returned to the factory, please ensure that is carefully and properly packed.
Okolab S.r.l. accepts no responsibility for damage due to unsatisfactory packing.
Upon examination of Okolab S.r.l., if the unit is found to be defective, it will be repaired or replaced at no
charge. This warranty does not apply to defects resulting from any actions of the purchaser. Okolab S.r.l.
neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result
from any action of the purchaser that discord from instructions listed in the operation manual. This warranty
does not cover or involve any other equipment that may be used along with the Okolab System (i.e. mini-
incubators, any gas tank, etc.), whose usage should be considered independent and performed according to
their own operational instructions.
Okolab S.r.l. warrants only the parts manufactured by it will as specified and free of defects. Okolab S.r.l.
makes no other warranties or representations of any kind whatsoever, express or implied, except that of
title, and all implied warranties including any warranty of merchantability and fitness for a particular
purpose are hereby disclaimed. LIMITATION OF LIABILITY: the total liability of Okolab S.r.l. shall not exceed
the purchase price of the component upon which liability is based. In NO event shall Okolab S.r.l. be liable
for consequential, incidental or special damages.
IST 872_REV 01
SV 1.0.0.14
