Manual de Servicio de Agilent

Maintaining YourAgilent LC andLC/MS Systems

Our measure is your success.

Agilent Systems for UHPLC and HPLC.............1

Pump Maintenance..........................................9Pump Maintenance Tips ....................................................................10Maintenance Procedures ..................................................................11Ordering Information .........................................................................19

Autosampler Maintenance ............................27Autosampler Maintenance Schedule ................................................28Maintenance Procedures ..................................................................28Ordering Information .........................................................................33

Fraction Collector Maintenance ....................39Fraction Collector Maintenance Schedule ........................................40Maintenance Procedures ..................................................................41Ordering Information .........................................................................42

Valve Maintenance ........................................45Valve Maintenance Notes .................................................................46Ordering Information .........................................................................46

Detector Maintenance...................................53Detector Maintenance Tips ...............................................................54Maintenance Procedures ..................................................................55Ordering Information .........................................................................59

LC/MS System Maintenance ........................69LC/MS Maintenance Schedule .........................................................70Ordering Information .........................................................................71

General Supplies ............................................79Ordering Information .........................................................................80

1Order online at www.agilent.com/chem/store

Welcome tothe next generation of HPLC

No matter what your application may be, the bottom line is results. And Agilent's UHPLCand HPLC platforms, combined with ZORBAX Rapid Resolution High Definition (RRHD)and ZORBAX Rapid Resolution High Throughput (RRHT) columns, help you achievethe best results.

This maintenance guide will help you keep your Agilent LC systems running at optimalperformance and peak efficiency. Inside this guide you'll find:

• Essential facts about Agilent supplies and accessories

• Vital maintenance procedures and troubleshooting tips

• All the Agilent part numbers you need in one easy-to-use guide

Agilent's UHPLC and HPLC Systemsare designed for the ultimate robustness,reliability, and performance.

2 Order online at www.agilent.com/chem/store

Agilent Systems for UHPLC and HPLC

Agilent 1290 Infinity LC System

An infinitely better UHPLC systemthat meets all your LC and LC/MS needs

The Agilent 1290 Infinity LC is designed to provide highest speed, resolution and sensitivity.A new power range allows you to deploy any particle type, any column dimensions, or anymobile and stationary phases. Innovative technology components offer the next level ofperformance for both UHPLC and HPLC applications. The 1290 Infinity LC is the first systemto provide the foundation for method transfer to or from any Agilent or non-Agilent UHPLCor HPLC system. In short, it gives you infinite capabilities to solve all your analytical challengesin LC and LC/MS.

Achieve stability up to 1200 bar for use with the Agilent 1290Infinity LC. Available in 50, 100 and 150 mm lengths for fast orhigh resolution separations of your most complex samples.

For more information, visitwww.agilent.com/chem/RRHD

ZORBAX Rapid Resolution High Definition(RRHD) 1.8 μm Columns



• Infinite power range of the Agilent 1290 Infinity LC combines ultra high pressure up to1200 bar and high flow rates up to 5 mL/min for maximum chromatographic performance,compatibility, flexibility and investment protection

• New 1290 Infinity Binary Pump combines active damping combined with lowest delayvolumes – facilitated by proprietary multi-layer technology in the new Agilent Jet WeaverMixer – to provide ultra-fast gradients and superior LC/UV and LC/MS performance

• New optical design in 1290 Infinity Diode Array Detector – including Agilent Max-LightCartridge Flow Cell – offers a new level of UV-sensitivity and baseline robustness, includingfast spectral acquisition at data rates up to 160 Hz

• Next generation flow-through design of the 1290 Infinity Autosampler achieves highestprecision for both large and small injection volumes without changing sample loops

• New 1290 Infinity Flexible Cube combines with Autosampler to facilitate fixed-loop injectionmode for ultra-fast cycle times, or automatic backflushing of needle seat for a newbenchmark in lowest carryover

• New Agilent Quick-Change Valves in the 1290 Infinity Thermostatted Column Compartmentboosts usability and paves the way for ultra high-throughput, multi-method and automatedmethod development solutions

Widest power range for infinitely betterperformance and flexibility

For more information,visit www.agilent.com/chem/1290

The power range of the Agilent 1290 Infinity LCis the widest available, combining ultra high pressures

up to 1200 bar and high flow rates up to 5 mL/minfor maximum chromatographic performance,

compatibility, flexibility and investment protection.

Agilent 1290 Infinity LC SystemDescription Part No.

1290 Infinity Binary Pump G4220A

1290 Infinity Autosampler G4226A

1290 Infinity Flexible Cube G4227A

1290 Infinity Thermostatted Column Compartment G1316C

1290 Infinity Diode Array Detector G4212A



Agilent 1200 SeriesRapid Resolution LC System

Discover the plus in performance

Increased productivity, confidence in analytical results and cost-effectiveness are keyobjectives in today’s analytical laboratories. The Agilent 1200 Series Rapid Resolution LC(RRLC) System is designed to meet these challenges by delivering significantly faster resultswith higher data quality.

On one system you can push RRLC performance to new limits and continue to run yourconventional methods, too. Discover now the plus in separation power and detectioncapabilities with new innovative system components, providing you with a new level of sampleinformation for more informed decisions in shorter time. Furthermore, the Agilent 1200 SeriesRapid Resolution LC System offers new levels of serviceability, uptime and compliance.

Get the maximum in speed, resolution, sensitivity and flexibility

• High resolution chromatography – 90,000 plates in 4 minutes

• Full compatibility with existing HPLC methods

• More detection capabilities – from UV-Visible and ELSD through LC/MS

• Near-zero sample carryover – for uncompromised data quality

• High system flexibility – for automated method development



Maximize your performance by combining the Agilent 1200 Series Rapid ResolutionSystem with Agilent ZORBAX Rapid Resolution HT columns. ZORBAX RRHT columnsuse a totally porous, 1.8 μm particle to provide maximum resolution in fast, ultra-fastand high resolution analyses.

Agilent ZORBAX Rapid Resolution High Throughput Columns

For more information, visitwww.agilent.com/chem/rrht

Agilent 1200 Series Rapid Resolution LC SystemDescription Part No.

1200 Series Micro Degasser G1379B

1200 Series Binary Pump SL G1312B

1200 Series High Performance Autosampler SL Plus G1367D

1200 Series Standard Autosampler SL G1329B

1200 Series Thermostat for Autosamplers (2x) G1330B

1200 Series Sample Capacity Extension G2257A

CTC Analytics HTC PAL Injection System G4270-CTC

CTC Analytics HTS PAL Injection System G4271-CTC

1200 Series Thermostatted Column Compartment SL G1316B

1200 Series Variable Wavelength Detector SL Plus G1314E

1200 Series Multiple Wavelength Detector SL G1365C

1200 Series Diode Array Detector SL G1315C

1200 Series Fluorescence Detector G1321A

1200 Series Evaporative Light Scattering Detector G4218A



Agilent 1200 SeriesStandard LC Systems

The leading industry-standard for quality,reliability and robustness

Whatever your liquid chromatography analysis needs are, you will find your individual solutionwithin the Agilent LC portfolio. The Agilent 1200 Series is the industry-leading standard,offering everything you need for analytical HPLC methodologies. From routine analysis throughnew product research and development in chemical analysis and pharmaceutical industries,the Agilent 1200 Series helps you achieve reliable and robust results for all your applications.Make a secure investment in the future of your lab with the scalable and open architecture ofthe Agilent 1200 Series HPLC solutions.

1200 Series Isocratic LC SystemThe LC workhorse for demanding QA/QC applications

• Entry-level system for world-class LC technology

• Easy and fast maintenance

• Flow rates up to 10 mL/min for a wide range of column dimensions and applications

• Upgrade options from isocratic to automated quaternary LC system

1200 Series Quaternary LC SystemFor routine method development and high-throughput gradient applications

• Greatest flexibility for multi-solvent gradients by accessing up to 4 solvents

• Flow rates up to 10 mL/min support narrow bore, standard andsemi-preparative applications

• Wide selection of autosamplers for full flexibility in injection volumes and sample containers



1200 Series Binary LC SystemFor research, high-throughput and fast applications

• High pressure gradient mixing for superior gradient performance even at lower flow rates

• Optimized delay volumes for fast chromatography

• Flow rates from 0.05 to 5 mL/min provide ideal support for narrow and standardbore applications

Related LiteratureOrder the Agilent HPLC Column Selection Guide from your local Agilent Representativeor Agilent Authorized Distributor using publication number 5990-4435EN.

Agilent 1200 Series Standard LC SystemsDescription Part No.

1200 Series Vacuum Degasser G1322A

1200 Series Micro Degasser G1379B

1200 Series Isocratic Pump G1310A

1200 Series Binary Pump G1312A

1200 Series Quaternary Pump G1354A

1200 Series Manual Injector G1328B

1200 Series High Performance Autosampler G1367B

1200 Series Standard Autosampler G1329A

1200 Series Thermostat for Autosamplers (2x) G1330B

1200 Series Thermostatted Column Compartment G1316A

1200 Series Variable Wavelength Detector G1314B

1200 Series Multiple Wavelength Detector G1365D

1200 Series Diode Array Detector G1315D

1200 Series Fluorescence Detector G1321A

1200 Series Refractive Index Detector G1362A

1200 Series Evaporative Light Scattering Detector G4218A



Related LiteratureOrder the complete 1200 Series brochure from your local Agilent Representativeor Agilent Authorized Distributor using publication number 5989-5200EN.

Agilent Rack for LC Systems

For more information, contact your local Agilent Representativeor Agilent Authorized Distributor.

Agilent rack for LC system, 5001-3726

This sturdy steel rack gives you fast, safe access to instruments and cables during maintenanceprocedures and module transfers. It is suitable for all Agilent LC module stacks, and features:

Agilent’s NEW LC system rack eliminatesclutter and keeps instruments secure

• Easy assembly that saves you time and trouble (see diagram below)

• Adjustable shelves that let you tailor the rack to suit your needs

• Open design that ensures proper airflow management and power distributionof equipment and cabling


Pump Maintenance

Regular pump maintenance will help you lower youroperating costs and generate precise results that makeyou feel confident.

You can count on Agilent isocratic, binary, quaternary, capillary, and preparative pumps forsuperior flow and composition stability. And by following a regular maintenance routine, youcan also count on maximum uptime and a steady, accurate solvent flow for the life of thepump.

On the following pages, you will find step-by-step instructions for these procedures:

• Exchanging the purge valve frit

• Replacing the active inlet valve cartridge

• Cleaning the solvent filter

• Exchanging the outlet ball valve

• Changing the pump seals

• Changing and inspecting the sapphire pistons

• Seal wear-in procedure

• Replacing the wash seals

You may perform these procedures all at once, or as needed. Keep in mind that some partsmay need replacing more often than others, depending on your application and solventpreparation procedures.


Pump Maintenance

Pump Maintenance Tips

Symptom Cause Solution

Solvent dripping out of waste outlet when valve closed Leak on pump head Exchange the purge valve frit or the purge valve

Pressure ripple unstable Dirty active inlet valve cartridge Run leak test for verification and exchange the activeinlet valve cartridge

Pressure ripple unstable Leak on pump head Run leak test for verification and exchange the outletball valve sieve or the complete valve

Gradient performance problems, intermittentpressure fluctuations

Solvent filter is blocked Change the solvent filter

A pressure drop of > 10 bar across the frit (5 mL/minH2O with purge valve open) indicates blockage

Dirty frit Exchange the purge valve frit or the purge valve

Leaks at lower pump head side High seal wear Run leak test for verification and exchangethe pump sealsUnstable retention time

Pressure ripple unstable

Seal life time shorter than normally expected Scratch on plunger Check plungers while changing the seals

Loss of wash solvent Leaky wash seals Exchange the wash seals

• Wrench 1/4-5/16 inch

• Wrench 14 mm

• Wrench 1/4 inch

• Pair of tweezersor toothpick

Tools required


Pump Maintenance

Maintenance Procedures

Exchanging the Purge Valve Frit

Maintenance Procedure

1. Using a 1/4 inch wrench, disconnect the pump outlet capillary at the purge valve.

2. Disconnect the waste tube. Beware of leaking solvents due to hydrostatic pressure.

3. Using a 14 mm wrench, unscrew the purge valve and remove it from the purgevalve holder.

4. Remove the plastic cap with the gold seal from the purge valve.

5. Use a pair of tweezers or a toothpick to remove the frit.

6. Place a new frit into the purge valve with the slit facing the gold seal.

7. Replace the cap with the gold seal.

Note: Before reinstallation, always check the gold seal. A deformed seal or a crackedcap should be replaced.

8. Insert the purge valve into the purge valve holder and orient the waste outletnozzle downward.

9. Tighten the purge valve and reconnect outlet capillary and waste tubing.


Pump Maintenance

Preparations

1. Switch off pump at the main power switch and unplug the power cable.

2. Unplug the active inlet valve cable from the connector.

3. Disconnect the solvent inlet tube at the inlet valve (beware of leaking solvents).

Note: Binary pumps without the solvent selection valve (SSV) have an adapter installedbetween the solvent line and the active inlet valve (AIV). Disconnect the solvent tubesat the adapter and remove the adapter from the AIV.


1. Using a 14 mm wrench, loosen the active inlet valve and remove the valve frompump head.

2. Use tweezers to remove the valve cartridge from the actuator assembly.

3. Clean the area in the actuator assembly. Flush the cartridge area thoroughly.

4. Insert a new valve cartridge into the actuator assembly (make sure the valve cartridgeis fully inserted into the actuator assembly).

5. Insert the valve into the pump head. Using the 14 mm wrench, turn the nut until it ishand tight.

6. Position the valve so that the solvent inlet tube connection points towards the front.

7. Using the 14 mm wrench, tighten the nut by turning the valve in its final position(do not overtighten the valve).

8. Reconnect the active inlet valve cable to the connector and the inlet tube to the valve.

Note: After an exchange of the valve cartridge, it may take several mL of pumping with thesolvent used in the current application before the flow stabilizes at a low percentage-rippleas low as when the system was working properly.

Replacing the Active Inlet Valve Cartridge

• Wrench 14 mm

• Pair of tweezers

Tools required

Active inlet valve body

Valve cartridge

Plug Active inlet valve Solvent inlet tube


1. Remove the blocked solvent filter from the bottle head assembly and place it in a beakerwith concentrated nitric acid (35%) for one hour.

2. Thoroughly flush the filter with bidistilled water (remove all nitric acid, some capillarycolumns can be damaged by nitric acid).

3. Replace the filter.


Pump Maintenance

Cleaning the Solvent Filter

If the filter is in good condition, the solvent will freely drip out of the solvent tube (hydrostaticpressure). If the solvent filter is partially blocked, only very little solvent will drip out of thesolvent tube.

Caution: Small particles can permanently block the capillaries and valves of the module.

• Always filter solvents

• Never use the module without solvent inlet filter


Pump Maintenance


1. Using a 1/4 inch wrench, disconnect the valve capillary from the outlet ball valve.

2. With a 14 mm wrench, loosen the valve and remove it from the pump body.

3. Remove the plastic cap with the gold seal.

4. Remove the sieve with a pair of tweezers.

Note: Check the gold seal. It should be exchanged when badly deformed. Place the valve inan upright position, insert the sieve into the recess and replace the gold seal with the cap.Make sure that the sieve cannot move and is away from the gold seal area.

5. Place a new sieve into the recess of the outlet ball valve and replace the cap withthe gold seal.

6. Reinstall the outlet ball valve and tighten the valve.

7. Reconnect the valve capillary.

• Wrench 1/4 inch

• Wrench 14 mm

• Pair of tweezers

Tools required

Valve body

Gold sealPlastic cap

Valve capillary

Outlet ball valve

Sieve (G1312A/B only)

Exchanging the Outlet Ball Valve

Before exchanging the outlet ball valve, you can try to clean it in a sonic bath. Remove thegold seal and the sieve. Place the valve in upright position (onto the plastic cap) in a smallbeaker with alcohol. Place in a sonic bath for 5 to 10 minutes. Insert a new sieve and replacethe gold seal.


Pump Maintenance

Sapphire piston and seals

Pistons and Seals

Agilent pistons are made from a high purity, monocrystalline sapphire for maximumdurability. Although ceramic pistons can be manufactured at a lower cost, ceramic isa sintered, polycrystalline material, which can cause undesirable variations during theproduction process. Agilent sapphire pistons are:

• Meticulously cut at just the right angle, making them the most durable – and longestlasting – pistons in the world

• Precisely aligned in their stainless steel holder to minimize wear on the piston and seal

Agilent seals are designed to fit snugly around our pistons, and are capable of adapting toa wide range of flow rates and pressures. Agilent piston seals are:

• Spring-loaded and engineered to deliver optimal performance over highly dynamic flowand pressure ranges

• Manufactured from a proprietary polymer blend, and feature a spring made from the samehigh-quality stainless steel that is used in our pump's flow path

The combination of our piston and seal has undergone extensive testing under temperaturestress, with all common HPLC solvents, and in many instruments. More importantly, they yieldconsistent, reproducible results.


Pump Maintenance

Each time you replace the seals, examine the pistons for scratches, which can cause smallbut damaging leaks.


All pumps except the G1361A preparative pump

1. Disassemble the pump head assembly.

2. Remove any deposits – particularly buffers – from the piston surface using alcohol ortoothpaste. Replace the pistons if scratched.

3. Rinse thoroughly in alcohol and HPLC-grade water.

4. Reassemble the pump head.

5. Check to make sure there are no fractures in the springs. Replace piston housingassembly if springs are damaged.

6. Reinsert the pistons.

Pump seals should be replaced when there are leaks on the bottom of the pump head,when retention times are inconsistent or when the pressure ripple is unstable.


All pumps except the G1361A preparative pump and the G4220A Infinity pump

1. Disassemble the pump head and use a piston to remove the two seals.

2. Clean the pump chambers and seal areas by flushing with solvent and wiping witha lint-free cloth.

3. Insert new seals.

4. Reassemble the pump head.

5. Perform the seal wear-in procedure (standard seals 5063-6589 only).

Note: The wear-in procedure involves flushing the seals with isopropanol at high pressurewhile they are in place.

Changing the Pump Seals

Changing and Inspecting the Sapphire Pistons


Pump Maintenance

Important: Do not perform this procedure when installing normal phase seals (0905-1420).It will cause severe damage to the seals.


Recommended after replacing standard seals only (5063-6589)

1. Fill the bottle with 100 mL of isopropanol, and place it in the solvent cabinet. Then, placethe new pump head tubing (including bottle head assembly) into the bottle.

2. Screw the adapter to the active inlet valve. Connect the inlet tube from the bottle headdirectly to the adapter (quaternary pumps and binary pumps with selection valves only).

3. Connect the restriction capillary to the purge valve. Insert the other end of the capillaryinto a waste container.

4. Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rateof 2 mL/min.

5. Close the purge valve and set the flow to a rate that is adequate to achieve a pressureof 350 bar. Pump for 15 minutes at this pressure to wear in the seals. You can monitorthe pressure at your analog output signal using the handheld controller, ChemStationor any other controlling device connected to your pump.

6. Turn OFF the pump flow and slowly open the purge valve to release pressurefrom the system.

7. Disconnect the restriction capillary and reconnect the outlet capillary to the purge valve.Reconnect the appropriate tubing to the active inlet valve.

8. Rinse your system with the solvent that you plan to use for your next application.

Seal Wear-In Procedure

• Solvent bottle or beaker

• Isopropanol

• AIV adapter (0100-1847)

• Restriction capillary(5022-2159)

Tools required


Pump Maintenance


1. Switch off binary pump at power switch.

2. Remove the seal keeper and the seal wash support rings from the plunger housing.Remove the seal keeper from the support ring assembly.

3. Using the blade of a flat head screwdriver, remove seal wash gasket and the secondaryseal from the support ring.

4. Use the insert tool to press the seal (spring pointing upwards) into the recess of thesupport ring. Place a seal wash gasket in the recess of the support ring and replacethe seal keeper.

5. Reassemble the pump head assembly.

Tools required Replacing the Wash Seals

• Hexagonal key 3 mm

• Hexagonal key 4 mm

• Insert tool

• Small flat headscrewdriver


Pump Maintenance

Purge valve assembly, G1311-60009

Purge ValveDescription Comments Part No.

Purge valve assembly For 1120 RRLC pump G4280-60031

Purge valve assembly, 400 bar For 1100/1200 pumpsIncludes PTFE frit

G1311-60009

Purge valve assembly, 600 bar For 1200 RRLC pumpIncludes PTFE frit

G1312-60023

5 position/7 port rotor seal For 1290 purge valve 5068-0005

5 position/7 port stator For 1290 purge valve 5068-0004

Purge valve actuator For 1120 RRLC pump G4280-60033

PTFE frits, 5/pk For 1100/1120/1200 RRLC,1120 and 1290 pumps

01018-22707

Seal for purge valve For 1120 RRLC pump 0905-1192

Gold seal, outlet For 1100/1200/1200 RRLC,1120 and 1290 pumps

5001-3707

Outlet caps, 4/pk For 1100/1200/1200 RRLCand 1120 pumps

5062-2485

Ordering Information

PTFE frits, 01018-22707


Pump Maintenance

Cartridge, 400 bar, 5062-8562

Outlet ball valve, G1311-60012

Active inlet valve, G1312-60025

Inlet ValvesDescription Comments Part No.

Active Inlet Valve (AIV)

Active inlet valve, without cartridge For 1100/1200/1200 RRLC pumps G1312-60025

Cartridge for active inlet valve, 400 bar For 1100/1200 pumps 5062-8562

Cartridge for active inlet valve, 600 bar For 1200 RRLC pump G1312-60020

Gold seal for inlet valve For 1100/1200/1200 RRLCand 1120 pumps

5001-3708

Passive Inlet Valve (PIV)

Passive inlet valve For 1120 Compact LC System G4280-60005

Inlet valve For 1290 Infinity LC G4220-60022

Valve

Valve assembly, inlet/outlet For 1200 Prep Pump G1361-60012

Outlet Ball ValveDescription Comments Part No.

Outlet ball valve For quaternary, isocratic 1100/1200 and 1120 pumps G1311-60012

For binary pump, 400 bar G1312-60012

For binary SL pump, 600 bar G1312-60022

For 1290 Infinity LC G4220-60028

Gold seal, outlet For 1100/1200/1200 RRLC and 1120 pumps 5001-3707

Outlet caps, 4/pk For 1100/1200/1200 RRLC and 1120 pumps 5062-2485

Sieves for outlet ballvalve, 10pk

For G1312-60012 valve 5063-6505


Pump Maintenance

Solvent reservoirs, 9301-1421

Solvent Reservoir and SuppliesDescription Part No.

Solvent Reservoir

Solvent reservoir, 1 L 9301-1420

Solvent reservoir, 1 L with cap 9301-1421

Solvent reservoir, 1 L 9301-0656

Solvent reservoir, amber, 1 L 9301-1450

Solvent bottle, clear, 2 L, 2 inlets 5065-4421

Solvent bottle, amber, 2 L 9301-6341

Solvent bottle, clear, 2 L 9301-6342

Bottle Head Assembly

Bottle head assembly for screw bottle G1311-60003

Bottle head assembly for F29/32 tapered solvent bottle G1312-68716

Bottle head assembly with tubing and filterFor capillary and nano systems (with stainless steel solvent filter)

G1376-60003

Bottle head assembly for prep system G1361-60022

Bottle head assembly for 1290 Infinity LC System G4220-60007

Bottle cap with 3-hole insert 5063-6531


Pump Maintenance

Pistons and SealsPiston Description Comments Part No. Seal Description Part No.

Sapphire piston For 1100/1120/1200/1200 RRLC pumps 5063-6586 Piston seals, graphite filled PTFE(reversed phase), 2/pk

5063-6589

Piston seals, polyethylene (normal phase) 0905-1420

Piston For 1290 Infinity LC 5067-4603 Pump seal PE for 1290 Infinity Binary Pump 0905-1719

Sapphire plunger For 1200 Prep Pump G1361-22402 Piston seal for G1361A Preparative Pump 5022-2188

Solvent FiltersDescription Recommended Use Part No. Frit Adapter Part No.

Glass filter, solvent inlet,20 μm pore size

Analytical scale, micro scale 5041-2168 Frit adapter, PTFE, 3 mm, 4/pk 5062-8517


Preparative LC 3150-0944 Frit adapter, PTFE, 4 mm G1361-23204


For G2258A Dual Loop Autosampler 3150-0944 Frit adapter, PTFE,for 1/8 in. OD tubing

G2258-23201

Stainless steel filter For use in capillary and nano systems 01018-60025


Pump Maintenance

Peristaltic pump, 5042-8507

Seal WashDescription Comments Part No.

Peristaltic pump cassettewith silicone tubing

For 1100/1200/1200 RRLC pumps 5042-8507

Silicone tubing, 1 mm ID,3 mm OD, 5 m

5065-9978

Peristalitic pumpwith ChemSure tubing

For 1100/1200/1200 RRLC pumps 5065-9952

ChemSure tubingfor peristaltic pump

5042-8954

Wash Seal and Wash Keeper

Wash seal For 1100/1200/1200 RRLC and 1120 pumps 0905-1175

Seal keeper For 1100/1200/1200 RRLC and 1120 pumps 5001-3743

Wash seal gasket, 6/pk For 1100/1200/1200 RRLC and 1120 pumps 5062-2484

Wash seal PE For 1290 Infinity Binary Pump 0905-1718

Support ring For 1290 Infinity Binary Pump with seal wash G4220-63010

Backup ring for sealholder

For 1290 Infinity LC Pump G4220-24013

Seal Wash Kit

Active seal wash kitfor isocratic orquaternary pumps

Includes 2 wash seal gaskets, 2 pump seals, peristalticpump (includes pump cassette and motor), 2 sealkeepers, 2 support ring assemblies, seal insert tooland silicone tubing

G1311-68711

Active seal wash kitfor binary pumps

Includes 4 wash seal gaskets, 4 pump seals,2 peristaltic pumps (includes pump cassette andmotor), 4 seal keepers, 4 support ring assemblies,seal insert tool and silicone tubing

G1312-68711

Continuous seal wash kit Includes 2 wash seal gaskets, 4 m flex tubing, 2 pumpseals, 1 flow regulator, 2 seal keepers, 2 support ringassemblies, 20 mL luer lock syringe, seal insert tooland abrasive paper

01018-68722


Pump Maintenance

Plastic tubing cutter, 8710-1930

Ferrules and rings, 5063-6598

PPS nuts, 5063-6599

Vacuum DegassersDescription Comments Part No.

Tubing kit, degasser to pump, 4/pk, 30 cm piecesof tubing with screws and bushings

For G1322A, G1379A/B G1322-67300

Mounting tool for flangeless nut For G1322A, G1379A/B 0100-1710

Plastic tubing cutter For G1322A, G1379A/B 8710-1930

Tefzel ferrules and SS lock rings, 1/8 in., 10/pk For G1322A, G1379A/B 5063-6598

PPS nuts, 1/8 in., 1/4-28 thread, 10/pk For G1322A, G1379A/B 5063-6599

Union, 1/4-28 thread, polypropylene For G1322A, G1379A/B 5022-2155

PTFE solvent tubing, 5 m, 1.5 mm ID, 3 mm OD For G1322A, G1379A/B 5062-2483

Disposable syringes, 20 mL, 10/pk For G1322A 5062-8534

Syringe adapter, 1/16 in. OD, 2 in. long For G1322A 9301-1337

Dual channel micro degasser vacuum chamber For G1379A G1379-60010

Micro vacuum degasser tubing kit for G1379A For G1379A G1379-67310

Micro vacuum degasser tubing kit for G1379B For G1379B 5042-8922

Online degasser accessory kitIncludes 8 screws, 8 bushings, 4 markers, tubing,syringe and syringe adapter

For G1322A, G1379A/B G1322-68705


Pump Maintenance

Maintenance KitsDescription Comments Part No.

Start-up Kits

Pump start-up kitFor 1100/1200/1290 RRLC pumps

Includes 1 outlet cap, 5 PTFE frits, 4 piston seals, 1 outlet gold seal, 2 glass solvent inletfilters, 20 μm, and 1 cartridge for active inlet valve

G1311-68710

Nanoflow LC start-up kit Includes PEEK coated fused silica capillaries, column and fittings to start upa Nanoflow LC System

G2228-68700

Seal Wash Kits

Active seal wash kit for isocratic orquaternary pumps

Includes 2 wash seal gaskets, 2 pump seals, peristaltic pump (includes pump cassetteand motor), 2 seal keepers, 2 support ring assemblies, seal insert tool and silicone tubing

G1311-68711

Active seal wash kit for binary pumps Includes 4 wash seal gaskets, 4 pump seals, 2 peristaltic pumps (includes pump cassetteand motor), 4 seal keepers, 4 support ring assemblies, seal insert tool and silicone tubing

G1312-68711

Continuous seal wash kitFor 1100/1200/1290 RRLC pumps

Includes 2 wash seal gaskets, 4 m flex tubing, 2 pump seals, 1 flow regulator, 2 sealkeepers, 2 support ring assemblies, 20 mL luer lock syringe, seal insert tool and abrasivepaper

01018-68722

Preventive Maintenance Kits

PM kit for isocratic/quaternary pump Includes piston seal, PTFE frits, 2 gold seals and 2 outlet caps G1310-68730

PM kit for binary pump Includes 4 piston seals, PTFE frits, 3 gold seals, 2 sieves and 3 outlet caps G1312-68730

Extended PM kitFor 1100/1200/1290 RRLC pumps

Includes 5063-6589 piston seals (2), 01018-22707 PTFE frits (5/pk), 5062-8562 cartridgeactive inlet valve, G1311-60012 outlet ball valve and 5063-6586 pistons (2)

5065-4499

Capillary pump PM kit Includes 4 pump seals, 4 stainless steel sieves, 4 gold seals, 1 stainless steel fritand 4 outlet caps

G1376-68710

PM kit for prep pump Includes 3150-0942 filter, 5022-2192 filter assembly, 5065-4445 peristaltic pump,0890-1764 tubing and 4 prep pump seals

G1361-68710

PM kit for seal wash option Includes 2 wash seals and 1 pack of 6 wash seal gaskets G1310-68731

PM kit for manual injector For 1120 manual injector systemsIncludes piston seals, PTFE frits, gold seals and rotor seal

G4280-68710

PM kit for automated injector For 1120 automated injector systemsIncludes piston seals, PTFE frits, gold seals, rotor seal, needle and needle seat

G4280-68730


Pump Maintenance

Accessory Kits

1100/1200 pump accessory kit Includes 3 wrenches, 5 PTFE frits, tubing, capillary and wrist strap G1311-68705

Pump configuration kit for G1312BNeeds G1158B 2 position/6 port valve

Includes side cover with fixed rail, top and right cover for pump housing, plus 6 connectingcapillaries. Allows automatic switching between different delay volumes to optimizethe system for 2.1 mm ID or 4.6 mm ID columns.

G1312-68726

Capillary pump accessory kit Includes purge valve and holder, hex keys 2.5, 3 mm, 2 wrenches 1/2 in. x 1/16 in.,wrenches 1/4 in. x 5/16 in. and 14 mm, wrist strap, torque adaptor and stainlesssteel frit, 0.5 μm

G1376-68705

Accessory kit prep pump/gradient G1361A Includes stainless steel connecting capillaries, solvent mixer, 2 L solvent bottle, bottle headassembly, filter, glass stop valve, stainless steel union, tubing and other parts

G1361-68707

Online degasser accessory kit Includes 8 screws, 8 bushings, 4 markers, tubing, syringe and syringe adapter G1322-68705

Extended flow range kit, 100 μL/min Includes all parts to go from 20 μL/min to 100 μL/min flow rate in a capillary LC system G1376-68707

Manual prep injection valve kit,stainless steel

Includes position sensing, 10 mL loop, 25 mL syringe, ring mounting bracket, start cableand SS connecting capillaries, 0.5 mm ID, 40 cm and 60 cm

5065-9922

Description Comments Part No.

Maintenance Kits (Continued)


Your Agilent autosampler is designed to deliver accurate measurements, precise injectionvolumes, and high-quality data. And by following a regular schedule of preventivemaintenance, you can ensure a lifetime of defensible results.

This section will give you step-by-step instructions on how to perform crucial proceduressuch as:

• Exchanging the needle

• Replacing the seat assembly

• Changing the rotor seal

You will also find the high-quality Agilent supplies you need to keep your autosampler runningat top efficiency.

Autosampler Maintenance

Ensuring peak performance, productivity,and peace-of-mind.




Warning: To avoid personal injury, keep fingers away from the needle area duringautosampler operation.

• Wrench 1/4 inch(supplied in accessory kit)

• Hexagonal key 2.5 mm(supplied in accessory kit)

• Pair of pliers

Exchanging the Needle

Tools required

Preparations

1. Select "Start" in the maintenance function "Change Needle."

2. When the needle is positioned approximately 15 mm above the needle seat, removethe front cover.

Autosampler Maintenance Schedule

Procedure Typical Procedure Time Required

Exchanging the needle assembly When needle shows indication of damage or blockage 15 minutes

Exchanging the seat assembly When the seat shows indication of damage or blockage 10 minutes

Exchanging the metering seal When autosampler reproducibility indicates seal wear 30 minutes




1. Select "Needle Down" until the needlescrew is aligned with the hole in thesafety cover.

2. Remove the sample loop fitting fromthe needle fitting.

3. Loosen the fixing screw and lift outthe needle.

4. Insert the new needle. Align theneedle in the seat, then tightenthe screw firmly.

5. Reconnect the sample loop fitting tothe needle fitting.

6. Use "Needle Up" to lift the needleto a position approximately 2 mmabove the seat.

7. Ensure the needle is aligned withthe seat.

8. On completion of this procedure,install the front cover.

9. Select "End" in the maintenancefunction "Change Needle."



Exchanging the Seat Assembly


• Flat head screwdriver

Tools required

Preparations

1. Select "Start" in the maintenance function "Change Needle."

2. Remove the front cover.

3. Use the "Needle Up" command in the "Change Needle" function to lift the needlean additional 1 cm.


1. Disconnect the seat capillary fittingfrom the injection valve (port 5).

2. Use a small flat head screwdriver toease out the needle seat.

3. Insert the new needle seat assembly.Press the seat firmly into position.

4. Connect the seat capillary fitting to port5 of the injection valve.

5. Use "Down" to position the needleapproximately 2 mm above the seat.

6. Ensure the needle is aligned withthe seat. If required, bend theneedle slightly until the needleis aligned correctly.

7. On completion of this procedure,install the front cover.

8. "Select "End" in the maintenancefunction "Change Needle."




• Hex key 9/64 inch(supplied in accessory kit)

Caution: The stator face is held in place by the stator head. When you remove the statorhead, the stator face can fall out of the valve. Carefully handle the valve to prevent damageto the stator face.

Replacing the Rotor SealTools required


1. Remove all capillary fittings from theinjection valve ports.

2. Loosen each fixing bolt two turns at atime. Remove the bolts from the head.

3. Remove the stator head, stator faceand stator ring.

4. Remove the rotor seal and isolation seal.

5. Install the new rotor seal and isolationseal. Ensure the metal spring insidethe isolation seal faces towards thevalve body.

6. Install the stator ring with the shorterof the two pins facing towards you atthe 12 o'clock position. Ensure the ringsits flat on the valve body.

(Continued)



7. Place the stator face in place on thestator head.

8. Install stator head and stator face.Tighten the bolts alternating two turnsat a time until the stator head is secure.

9. Reconnect the pump capillaries to thevalve ports.

10. Slide the waste tube into the wasteholder in the leak tray.

11. On completion of this procedure, installthe front cover.



Injection Valves

Valve Use With Part No.Rotor SealMaterial

Rotor SealPart No.

StatorPart No.

Stator FacePart No.

RheBuild KitPart No.

2 position/6 port injection valve G1313A,G1329A,G1367A, 1120

0101-0921 Vespel 0100-1853 0100-1850 0100-1851 0101-1257

Tefzel 0100-1849

PEEK 0100-2231

2 position/6 port micro injection valve G1377A,G1389A

0101-1050 Vespel 0100-2088 0100-2089

2 position/6 port injection valve,600 bar

G1367C SL,G1367D SL Plus,G1329B

0101-1422 PEEK 0101-1416 0101-1417

10 port, dual loop valve G2258A 0101-1385 Vespel 0101-2415 0101-1390

2 position/6 port MBB injection valve G2260A 0101-1267 PEEK 0101-1268* 0100-2195 0101-1268

2 position/6 port ultra high pressurevalve, 1200 bar

G4226A 5067-4117 Vespel 5068-0007 5068-0006

*Includes seal and stator face




Needles and Needle SeatsAgilent Autosampler Needle Assembly Description Part No. Compatible with Needle Seat Part No.

G1313A, G1329A, G1120A(automated sampler only)

Needle assembly, standard autosampler G1313-87201 Standard needle seat0.17 mm ID capillary, 2.3 μL

G1313-87101

Standard needle seat0.12 mm ID capillary, 1.2 μL

G1313-87103


Needle assembly, for use with PEEK seat G1313-87203 Standard needle seat, PEEK0.17 mm ID capillary, 2.3 μL

G1313-87102


Needle assembly, 900 μL upgrade G1313-87202 Standard needle seat0.17 mm ID capillary, 2.3 μL

G1313-87101

G1389A Needle assembly, micro LC autosampler G1329-80001 Micro LC needle seat100 μm ID capillary, 1.2 μL

G1329-87101

Micro LC needle seat50 μm ID capillary, 0.3 μL

G1329-87103

G1367A/B Needle assembly,well plate autosampler (green)

G1367-87200 Needle seat, well plate autosampler0.17 mm ID capillary, 2.3 μL

G1367-87101

Needle assembly,well plate autosampler (new, blue)

G1367-87201 Needle seat, well plate autosampler0.12 mm ID capillary, 1.2 μL

G1367-87102

G1367C SL Needle assembly,well plate autosampler

G1367-87201 Needle seat, 600 bar, without capillary G1367-87104

Seat capillary, 0.17 x 100 mm,0.8 mm OD

G1367-87302

Seat capillary, 0.12 x 100 mm,0.8 mm OD

G1367-87303

G1367D SL Plus Needle assembly,well plate autosampler (black)

G1367-87202 Needle seat, 600 bar, with seat capillary,0.17 mm ID x 100 mm, 0.8 mm OD

G1367-87017

Needle seat, 600 bar, with seat capillary,0.12 mm ID x 100 mm, 0.8 mm OD

G1367-87012

G1377A Needle assembly,micro well plate sampler

G1377-87201 Micro needle seat with seat capillary,100 μm

G1377-87000

Micro needle seat with seat capillary,75 μm

G1377-87001

Micro needle seat with seat capillary,50 μm

G1377-87002

G2258A Needle assembly, dual loop autosampler G2258-68710 Twin needle seat, dual loop autosampler G2258-87102

G2260A Needle assembly, prep autosampler G2260-87201 Needle seat, prep autosampler0.5 mm ID, 20 μL

G2260-87101

G1313A, G1120A(automated sampler only)

Seat adapter G1313-43204

G1313A, G1329A,G1389A, G1120A(automated sampler only)

Finger caps, 15/pk 5063-6506

G1377A Tool for micro seal capillary mounting G1377-44900

G4226A Needle assembly,1290 Infinity Autosampler

G4226-87201 Seat assembly, 0.12 mm,1290 Infinity LC

G4226-87012

Agilent Autosampler Needle Assembly Description Part No. Compatible with Needle Seat Part No.

Needles and Needle Seats



(Continued)



Piston Description Use With Part No. Seal Description Part No.

Sapphire piston, 40 μL G1367D, G1389A,G1377A, G4226A

5064-8293 Piston seal, 2 mm, for G1367D, G1389A, G1377A 5022-2175

Piston seal for G4226A 0905-1717

Sapphire piston, 100 μL G1313A, G1329A,G1120A, G1367A/B/C

5063-6586 Piston seals, graphite filled PTFE (reversed phase), 2/pk 5063-6589

Sapphire piston, 900 μL G1313A, G1329A, 1120 5062-8587 Metering valve seal 0905-1294

Piston, 5 mL G2258A G2258-60003 Piston seal 0905-1599

Metering Device Supplies

Infrequently, the metering device seal and piston may need replacement if you see loss ininjection volume precision or metering device leaking.



Vial plate, G2255-68700 Tray, 5022-6538

Autosampler TraysDescription Comments Part No.

100 position tray for 2 mL vials For G1313A,G1329A, G1120A

G1313-44510

100 position tray for 2 mL vials, thermostattable G1329-60011

40 position tray for 2 mL vials G1313-44512

15 position tray for 6 mL vials G1313-44513

External vial tray for 17 vials (disposal position) G1313-60004

Disposal tube for external vial tray G1313-27302

Well plate tray, 2 well plates, 10 vials (supports 50 mm plates) For G1367A/B/C/D,G2258A, G4226A

G2258-60011

Vial plate for 54 x 2 mL vials, 6/pk G2255-68700

Vial plate for 15 x 6 mL vials 5022-6539

100 position tray for micro vials For G1367A/B/C/D,G4226A

G4226-60021

Tray for 27 Eppendorf safe lock tubes, 0.5/1.5/2 mL For G1367A/B/C/D 5022-6538

8.5 in. well plate rack, 2/pkFor 16 shallow well plates, 4 deep well plates (max 48 mm height) or 6 vial racks

For G2257A G2255-68709

10 in. well plate rack, 2/pkFor 20 shallow well plates (max height 16 mm), not compatible with deep well plates

G2255-68710

8.5 in. well plate rack extensionIncludes 3 racks for 3 x 16 shallow well plates, 2 x 4 deep well plates (max 48 mm height) or 3 x 6 vial racks

G2255-68720

10 in. well plate rack extensionIncludes 3 racks for 3 x 20 shallow well plates (max height 16 mm), not compatible with deep well plates

G2255-68730

205H rack, two 96 deep well plates For G2250A G2250-04504

200 rack, 13 x 100 mm tubes (9 mL), 96 G2250-04503



94A special holding 1100 tray G2250-04500



Autosampler KitsDescription Comments Part No.

PM kit for 1200 autosampler SL Includes PEEK rotor seal, needle seat, needle, 2 seals and 15 finger caps G1329-68719

G1313/27A autosampler maintenance kit Includes 1 rotor seal (Vespel), 1 needle, 1 needle seat, 2 metering seals and 15 finger caps G1313-68709

G1313/27A autosampler accessory kit Includes 3 hex keys, 2 wrenches, tubing, 1 label halftray, wrist strap, 15 finger caps,0.17 ID x 180 mm capillary, 100 screw top 2 mL vials and caps, tray for 40-2 mL vialsand tray for 15-6 mL vials

G1313-68705

PM kit for standard autosampler Includes 1 each: rotor seal, isolation seal, stator face, needle and needle seat 5065-4498

PM kit for standard autosampler Includes Vespel rotor seal, standard needle seat and needle G1313-68730

Light protection kit for G1329A Includes opaque front and side doors and front cover G1329-68718

Door replacement kit for G1329A Includes transparent front and side doors G1329-68727

Well plate sampler accessory kit Includes 100/pk 2 mL screw top vials and caps, 10/pk 0.5 mL 96 well plates, tools,380 mm 0.17 mm capillary and CAN cable

G1367-68705

PM kit for well plate autosamplers Includes Vespel rotor seal, needle, needle seat, peristaltic pump cartridge and seal tight nut G1367-68730

Micro well plate sampler accessory kit Includes 100/pk 2 mL screw top vials and caps, 10/pk 0.5 mL 96 well plates, tools,40 μL loop, 50 cm-50 μm fused silica/PEEK capillary, 75 μm seat capillary and CAN cable

G1377-68705

Accessory kit for G2260A prep autosampler Includes stainless steel connecting capillaries, hex keys, wrenches, 100/pk 2 mL screw capvials and caps, tray for 6 mL vials, stainless steel union and other parts

G2260-68705

Multidraw upgrade kit forG1313A/G1327A/G1329A autosamplers

Includes 500 μL capillary, 1500 μL capillary and ZDV union G1313-68711

PM kit, manual injector For 1120 manual injector systemsIncludes piston seals, PTFE frits, gold seals and rotor seal

G4280-68710

PM kit, auto injector For 1120 automated injector systemsIncludes piston seals, PTFE frits, gold seals, rotor seal, needle and needle seat

G4280-68730


When you are under pressure to generate better results faster, there is no time to compromiseon recovery and purity. And with Agilent’s 1100 and 1200 Series Fraction Collectors, you willnot have to.

Agilent fraction collectors are designed to process data in real time for instantaneousand precise fraction collection while increasing throughput on your purification system.So you can be certain that you are getting the highest degree of recovery and purity foryour fractions – even with low flow rates.

In this section, you will find essential details about our analytical scale (AS) and preparativescale (PS) fraction collectors, including:

• Fraction collector maintenance, including simple repairs you can perform withouta service call

• Guidelines for maintaining the micro fraction collector and spotter

• Agilent-engineered parts, such as trays, collecting tubes, plates, and needle assemblies

• MALDI spotting accessories

Fraction Collector Maintenance

A higher level of purityfor results that stand up to scrutiny.



Fraction CollectorMaintenance Schedule

Procedure When to Perform

Analytical and Preparative Fraction Collector Maintenance

Replace the inlet/waste tubing Once per year – or when you notice signsof damage or wear

Replace the valve-to-needle tubing Once per year – or when you notice signsof damage or wear

Exchange the preparative needle assembly When the needle shows signs of damageor blockage

Exchange the analytical needle assembly When the needle shows signs of damageor blockage or when using the short needleassembly with high test tubes (>45 mm)

Exchange the diverter valve When the valve is leaking or notswitching properly

Exchange the internal tray When the flow delay sensor no longer works

Repair or exchange a funnelwithin the internal tray or funnel tray

When defective, leaky, blocked or contaminated

Micro Fraction Collector/Spotter Maintenance

Replace fraction collector capillary At least every six months or when worn,blocked or damaged

Exchange the capillary guiding assembly When bent or damaged

Exchange the internal tray When the flow delay sensor no longerworks properly

Exchange the flap septum and waste tubing At least every six months or when defectiveor contaminated




There are two types of tubing available to connect the valve with the needle: preparativescale PTFE tubing (0.8 mm ID) and analytical scale tubing (0.25 mm ID). Both types shouldbe replaced when they become contaminated, worn or damaged.

Agilent offers three types of connecting capillaries – each with different internal diameters.We recommend replacing the capillaries and flap septum every six months – or whencontaminated, blocked, worn, or visibly damaged.

Consult your user's manual for detailed replacement instructions.


1. Position the fraction collector's transport unit in the "Home" position.

2. Remove all installed trays from the tray base.

3. Position the transport unit in the "Change Parts" position.

4. Turn off the instrument.


1. Place the transport unit of the micro fraction collector/spotter in the "Home" position.

2. Remove all installed trays from the tray base.

3. Place the transport unit in the "Change Parts" position.

4. Turn off the instrument.

5. Unscrew the micro fraction collector/spotter capillary from the flow cell of the detector.

Tubing Replacement

Capillary Replacement



Fraction Collector Capillary Kits and NeedlesModule Max Flow Rate Tubing ID (mm) Tubing Kit Needle Length Needle Typical Use

G1364B 100 mL/min 0.8 mm ID G1364-68711 G1364-87201 Tubes (max 100 mm)

G1364C 1 mL/min 0.15 mm ID G1364-68723 50 mm G1367-87200 Tubes (max 48 mm), well plates, vials

10 mL/min 0.25 mm ID G1364-68712 50 mm G1367-87200

10 mL/min 0.25 mm ID G1364-68712 20 mm G1364-87202 Funnel tray (tubes max 75 mm)

100 mL/min 0.8 mm ID G1364-68711 20 mm G1364-87202

G1364D 4 μL/min 25 μm ID G1364-87304 MALDI targets, well plates

4-30 μL/min 50 μm ID G1364-87305

30-100 μL/min 100 μm ID G1364-87306


Collecting Tubes and TraysTrayPart No. Hole Diameter (mm)

No. ofTubes Tube Dimensions

TubePart No. Unit

G1364-84523 30 40 30 x 48 mm 5042-6470 100/pk

G1364-84524 25 60 25 x 100 mm 5042-6459 100/pk

G1364-84525 16 126 16 x 48 mm 5022-6533 100/pk

G1364-84516 12 215 12 x 48 mm 5022-6534 100/pk

G1364-84532 Funnel tray 40 Any size



Collection plate, showing 96-position closing mat,5042-1389

Well Plate TraysDescription Part No.

Tray for 4 well plates, cooled G1364-84521

Tray for 4 plates, adjustable, cooled G1364-84531

Tray for 2 well plates, 10 funnels, cooled G1364-84522

Tray for 2 well plates, 10 vials, 2 mL G1367-60001

Well PlatesDescription Unit Part No.

96 well plates, 0.5 mL, polypropylene 120/pk 5042-1385

96 well plates, 0.5 mL, polypropylene 10/pk 5042-1386

96 deep well plates, 1 mL, polypropylene 50/pk 5042-6454

Closing mats for 96 well plates, silicone, pre-slit, fits 96 well plates5042-1385 and 5042-1386 only

50/pk 5042-1389

96 well plates, 150 μL, conical, polypropylene 25/pk 5042-8502

384 well plates, 90 μL, polypropylene 30/pk 5042-1388

384 well plates, max 45 μL, skirted 25/pk 5188-5375

96 deep well collection plates with glass inserts, caps, and septa,pre-assembled, 0.35 mL

5065-4402

Glass inserts, 350 μL 1000/pk 5188-5321

Caps/septa for glass inserts 1000/pk 5188-5322

Vial plate for 54 x 2 mL vials 6/pk G2255-68700

Tray for 27 Eppendorf safe lock tubes, 0.5/1.5/2 mL 5022-6538

Vial plate for 15 x 6 mL vials 5022-6539

Plate for 24 tubes with 18 mm diameter 5042-8544Tray, 5022-6538



MALDI spotting adapter, G1364-83205

Well plate adapter assembly, G1364-60021

Maldi plate carrier Bruker, 5022-6541

Calibration plate Bruker, 5023-0208

G1364D Micro Fraction Collector SuppliesDescription Part No.

MALDI spotting adapter for G1364D G1364-83205

Well plate adapter assembly for G1364C/D G1364-60021

Flap septum, PEEK, for internal tray G1364-27107

Fused silica/PEEK capillary, 25 μm, 50 cm G1364-87304



Waste tube, PTFE, 20 cm, 1.4 mm ID, 2.0 mm OD G1364-86711

MALDI plate carrier Bruker 5022-6541

MALDI plate carrier Bruker PAC 5022-6546

MALDI plate carrier ABI 5022-6542

MALDI plate carrier ABI Opti-TOF 5023-0238

MALDI plate carrier Agilent 5022-6543

MALDI plate carrier Micromass 5022-6544

Target plate for AP-MALDI LC/MS G1972-60025

Calibration plate Bruker 5023-0208

Calibration plate ABI 192 5023-0209

Calibration plate ABI 10x10 & 20x20 5023-0213

Calibration plate Agilent 5023-0214

Calibration plate Micromass 5023-0215

On-line matrix kit for MALDI spottingIncludes BCD board/cable, syringe, needles, adapters, connector and capillary

G1364-68706

Adapter, female to female 1/4-28 5042-8517

Adapter, male luer to female 1/4-28 5042-8518

Syringe, glass, 1 mL, 1/4-28 connector 5181-1541

Micro T-connector, PEEK, swept vol 29 nL, with 1/32 in. ID fittings 5042-8519

MALDI spotting tips, PTFE, 10/pk G1364-81701


Agilent's industry-leading Manual Injection Valves are designed to ensure trouble-freeoperation with your HPLC System.

Our valves also feature patented "Make-Before-Break" architecture that allows you to switchbetween LOAD and INJECT positions without interrupting the flow. So you can analyze moresamples in less time.

In this section, you will find the latest in LC injection valve technology, along with importantmaintenance information that will maximize reproducibility.

Valve Maintenance

Engineered for consistent resultsand optimal system performance.


Valve Maintenance

• PEEK offers a high chemical resistance and versatility and will tolerate the entire pH rangefrom 0 to 14.

• Tefzel is recommended for use in applications where PEEK cannot be used, such asmethylene chloride or DMSO in higher concentrations.

pH Range 0-7 7-10 10-14

Vespel

PEEK

Tefzel

Injection Valves

Valve Use With Part No.RheBuild KitPart No.

Rotor SealMaterial

Rotor SealPart No.

StatorPart No.

Stator FacePart No.

2 position/6 port injection valve G1313A,G1329A,G1367A, 1120

0101-0921 0101-1257 Vespel 0100-1853 0100-1850 0100-1851

Tefzel 0100-1849

PEEK 0100-2231

2 position/6 port micro injection valve G1377A,G1389A

0101-1050 Vespel 0100-2088 0100-2089

2 position/6 port injection valve,600 bar

G1367C SL,G1367D SLPlus, G1329B

0101-1422 PEEK 0101-1416 0101-1417

10 port, dual loop valve G2258A 0101-1385 Vespel 0101-2415 0101-1390

2 position/6 port MBB injection valve G2260A 0101-1267 0101-1268 PEEK 0101-1268* 0100-2195

2 position/6 port ultra high pressurevalve, 1200 bar

G4226A 5067-4117 Vespel 5068-0007 5068-0006

*Includes seal and stator face

• Vespel is a polyimide with low wear and high chemical resistance. Vespel tolerates a pHrange of 0 to 10. More basic solutions dissolve Vespel, which damages the rotor seal.

Valve Maintenance Notes



Valve Maintenance

External Switching Valve Replacement Parts

Description Use WithRheBuild KitPart No.

Rotor SealMaterial

Rotor SealPart No.

StatorPart No.

12 position/13 port preparative solvent selection valve G1160A 0101-1288 0101-1365

6 position/14 port column selection valve(six column selector)

G1159A 0101-1290 0101-1364

2 position/10 port valve dual-sided MBB G1157A 0101-13590101-1289

0101-1362

2 position/6 port switching valve G1158A 0101-1358(with PEEKrotor seal)

Vespel 0100-1855 0100-1850

Tefzel 0100-1854

PEEK 0100-2233

2 position/6 port switching valve, 600 bar G1158B HP PEEK blend 0101-1409 0101-1417

2 position/6 port micro switching valve G1162A 0100-2087 0100-2089

2 position/10 port micro switching valve G1163A 0101-1361 0101-1363

6 position/7 port selection valve G1156A 0101-1361 0101-1410

Internal Switching Valves

Description Use With Valve HeadRotor SealMaterial

Rotor SealPart No.

StatorPart No.

2 position/6 port column switching valve head, 400 bar G1316A 0101-0920 Tefzel 0100-1854 0100-1851

Vespel 0100-1855

PEEK 0100-2233

2 position/6 port ultra high pressure valve head, 1200 bar G1316C 5067-4117 5068-0008 5068-0006

2 position/6 port micro column switching valve head, 400 bar G1316A 0101-1051 0100-2087 0100-2089

8 position/9 port low pressure valve head, 400 bar G1316C 5067-4108 5067-4113 5067-4112

8 position/9 port high pressure switching valve head, 600 bar G1316C 5067-4107 5067-4111 5067-4110



2 position/10 port switching valve head, 400 bar G1316A 0101-1343 0101-1361 0101-1362

2 position/10 port micro column switching valve head, 600 bar G1316B 0101-1419 0101-1415 0101-1421


Valve Maintenance

Agilent provides the latest developments in LC injection technology from Rheodyne.

• Continuous flow path with "Make-Before-Break" design

• Sample capacity

• Choice of stainless or PEEK flow path

• Easy access to fittings due to wide 30° port angles

Manual Injection Valves with Position Sensing Switches

Description Comments Part No.Rotor SealMaterial

Rotor SealPart No.

StatorPart No.

Stator FacePart No.

RheBuild KitPart No.

7125 N/A Vespel 0101-0623 0101-0624

Tefzel 0101-0620

PEEK 0101-1255

7725i, stainless steel Analytical scale 5063-6502 PEEK 0101-1255 0100-1860 0100-1859 0101-1254

9725i, PEEK Analytical scale 0101-1253 PEEK 0101-1255

3725i, stainless steelprep valve

Preparative scale 0101-1232 PEEK 0101-1233

3725i, PEEKprep valve

Preparative scale 0101-1231 PEEK 0101-1233

7010/7000/7040 N/A PEEK 0101-1256

Manual prepinjection valve kit,stainless steel

Includes position sensing,10 mL loop, 25 mL syringe,ring mounting bracket, start cableand SS connecting capillaries,0.5 mm ID, 40 cm and 60 cm

5065-9922

2 position/6 portmanual injection valvefor 1120

Does not include 20 μL loopand needle port

5067-4104 Vespel 5067-4105 0100-1850

Manual Injection Valves


Valve Maintenance

The right mix of injection valve sample loops are available for your application needs.Agilent offers factory-cut and finished loops of the highest quality.

• Stainless steel loops are square cut, free of burrs, for a flush connection

• Flexible PEEK loops have a clean, straight cut for low dead volume connections

Manual Injection Valve Sample Loops

Manual Injection Valve Replacement Parts

Description Comments Part No.

Isolation seal For Rheodyne Series 7725, 9725, 3725 1535-4046

Ring stand mounting bracket 1400-3166

PEEK adapter, 1/8 in. to 1/16 in. For 3725i 5067-1503

Position sensor switch for manual valves 0490-1849

Volume ID (mm) Material Use With Part No.

5 μL 0.18 SS 7125 and 7010 1535-4860

0.18 SS 7725 0101-1248

0.18 PEEK 9725 0101-1241

10 μL 0.30 SS 7125 and 7010 0101-0376

0.30 SS 7725 0100-1923

0.25 PEEK 9725 0101-1240

20 μL 0.51 SS 7125 and 7010 0101-0377

0.30 SS 7725 0100-1922

0.25 PEEK 9725 0101-1239

50 μL 0.51 SS 7125 and 7010 0101-0378

0.51 SS 7725 0100-1924

0.51 PEEK 9725 0101-1238

100 μL 0.51 SS 7125 and 7010 0101-0379

0.51 SS 7725 0100-1921

0.51 PEEK 9725 0101-1242


Valve Maintenance

200 μL 0.76 SS 7125 and 7010 0101-1252

0.76 SS 7725 0101-1247

0.51 PEEK 9725 0101-1237

500 μL 0.76 SS 7125 and 7010 0101-1251

0.76 SS 7725 0101-1246

0.76 PEEK 9725 0101-1236

1 mL 0.76 SS 7125 and 7010 0101-1219

0.76 SS 7725 0101-1245

0.76 PEEK 9725 0101-1235

2 mL 1.00 SS 7125 and 7010 0101-1250

1.00 SS 7725 0101-1244

0.76 PEEK 9725 0101-1234

1.6 PEEK 3725 0101-1229

5 mL 1.00 SS 7125 and 7010 0101-1249

1.00 SS 7725 0101-1243

0.76 PEEK 9725 0101-1230

1.6 PEEK 3725 0101-1228

10 mL 2.0 PEEK 3725 0101-1227

20 mL 2.0 PEEK 3725 0101-1226

Volume ID (mm) Material Use With Part No.


Valve Maintenance

Syringes for Manual Injection

Syringes for manual injection

• Improved scale readability with a new vertical syringe scale orientation

• Unique package design that allows you to store syringes right on your lab bench

• Accuracy within ±1% of nominal volume, and with precision within 1%, measured at 80%of total scale volume

• Earth-friendly cardboard and recyclable plastic packaging

• Certificate of Conformance to ensure the highest quality construction and performance

• Lot traceable for accurate identification

LC Manual Syringes with Fitted Plungers

Volume (μL) Description Unit Needle Gauge/Length/Tip Part No.

5 Fixed 22 gauge/2 in./LC tip 5190-1480


Removable 22 gauge/2 in./LC tip 5190-1485

Replacement needlefor 10 μL syringe

3/pk 5190-1486






Agilent color-coded manual syringes allow you to determine syringe volume with one quickglance, so you can more efficiently perform manual dilution, extraction, and sample prep. Theyalso give you the advantages of:

New color-coded manual syringes let you identifycritical volumes at a glance and help boost productivityalong every step of your sample processing workflow

Related LiteratureTo select the best syringe for yourapplication, order the Agilent SyringeSpecifications and Selection Guide fromyour local Agilent Representative orAgilent Authorized Distributor usingpublication number 5990-3563EN.


Valve Maintenance

LC Manual Syringes with PTFE-Tipped Plungers

Volume (μL) Description Unit Needle Gauge/Length/Tip Part No.

10 Removable 22 gauge/2 in./LC tip 5190-1492

Replacement needle for10 μL syringe

3/pk 5190-1486

Replacement plunger withPTFE tip for 10 μL syringe

5190-1558


Replacement needle 3/pk 5190-1571


5190-1560




5190-1561




5190-1562






5190-1564


Agilent wavelength detectors combine exceptional flexibility with superior instrument control,data communication, and analytical capabilities. This section will show you how to maintainyour detector’s high level of selectivity and sensitivity by performing the following procedures:

• Choosing the most compatible flow cell for your application

• Disassembling, cleaning, and installing the flow cell

• Changing the Deuterium lamp

Of course, you will also find the precision-engineered Agilent parts you need to get the jobdone quickly and keep your downtime to a minimum.

Ensure uncompromised chromatographic performancewith these tips and techniques.

Detector Maintenance



Detector Maintenance Tips

Symptom What To Do Additional Information

Lamp does not ignite Exchange the lamp Perform a wavelength calibration test and an intensity test after thelamp replacement

Noise exceeds application limit Exchange the flow cell Perform a wavelength calibration test after the flow cell replacement

Drift exceeds application limit Exchange the lamp Perform a wavelength calibration test and a pressure tightness testafter the flow cell replacement

Leaky flow cell(For G4212 only)

Exchange the flow cell Perform a wavelength calibration test after the flow cell replacement

Leaky flow cell(For all G1314/G1315/G1365 detectors)

Clean or exchange the flow cell Perform a wavelength calibration test and a pressure tightness testafter the flow cell replacement

Lower intensity(For G4212 only)

Exchange the flow cell Perform a wavelength calibration test after the flow cell replacement

Lower intensity(For all G1314/G1315/G1365 detectors)

Clean or exchange the flow cell Perform a wavelength calibration test and a pressure tightness testafter the flow cell replacement



Tools required

Screwdriver (POZI 1 PT3) Preparations

1. Turn the lamp off.

Note: Allow lamp to cool down.

2. Remove the front cover and locate the lamp area.


1. Unscrew the three screws of the lamp housing cover and remove the cover.

2. Locate the two screws that secure the lamp and unscrew.

3. Disconnect the lamp connector and remove the lamp.

4. Place the lamp on a clean surface.

Note: Do not touch the glass bulb of the new lamp with fingers. Doing so mayreduce light output.

5. Insert the new lamp and reconnect the lamp connector.

6. Secure the lamp with the two screws.

7. Fit the lamp wires inside the lamp housing cover so that they are not scratchedby the cover.

8. Replace the lamp housing cover and secure the three screws.

Note: Perform a wavelength re-calibration after lamp warm up.


Replacing the Deuterium Lamp

Long Life Deuterium Lamp with RFID tag, 2140-0820



Preparations

1. Turn the pump off.

Note: If the flow cell has not been used for some time, flush it with isopropanol.

2. Disconnect the inlet capillary to CELL IN (left) and the waste tube to CELL OUT (right).


1. Unlock the cell cartridge holder by pulling the lever to the front. The lever should bein the final down position.

2. Pull the cell cartridge holder completely out towards the front.

3. Remove the cell from the cartridge holder.

4. Replace the black hoods (A) to the cell interfaces (IN/OUT) and insert plugs (B)for safe storage.

Note: The hoods and plugs should always be in place in order to protect the flow cell.For long-term storage, the flow cell should be flushed and filled with isopropanol to preventthe growth of algae.

5. Slide the cell cartridge holder completely into the module.

6. Lift the two levers into the upper final position to secure the cell.

7. Remove the plugs from the CELL IN and CELL OUT and set aside.

8. Connect the inlet capillary to CELL IN (left) and the waste tubing to CELL OUT (right).

Tools required

Hexagonal wrench

Replacing the Max-Light Cartridge Cellin a 1290 Infinity LC



Replacing the Flow Cell

Standard flow cell, G1315-60012

Flow Cell Maintenance (Procedure A)

1. Remove both capillaries from the flow cell.

2. Remove the flow cell from the holder.

3. Remove the cell screw with a 4 mm hex key.

4. Remove the gasket.

5. Use ethanol to clean the cell body.

6. Insert the new gasket.

7. Insert the new cell screw.

8. Carefully tighten the cell screw.

Flow Cell Maintenance (Procedure B)

1. Remove both capillaries from the flow cell.

2. Unscrew the cell screws and remove the flow cell.

3. Remove the screw with a 4 mm hex key.

4. Carefully remove the three washers and the gasket.

5. Carefully remove the cell window and the seal.

6. Use ethanol to clean the cell body.

7. Insert the new seal and the new cell window.

8. Insert the gasket and the three washers.

9. Insert the cell screw.

10. Carefully tighten the cell screw.



Installing the Detector Cell

Before you return the flow cell to the instrument module:

1. Connect the capillaries.

2. Switch on the pump and check the cell for leaks.

Once you are convinced the cell is leak free:

3. Install the flow cell into the instrument.

4. Screw down the cell.

We recommend the following tests after cell installation:

Zero-order calibration

Wavelength calibration

Because the flow cell is part of the optical system and has now been moved since these testswere last performed, we recommend running them again before your next analysis.



Long Life Deuterium Lamp with RFID tag, 2140-0820


Detector LampsDescription Comments Part No.

1100/1200 Series Variable Wavelength Detector (VWD)Long life Deuterium lamp with RFID tag For G1314D/E G1314-60101

Long life Deuterium lamp For G1314A/B/C/1120 G1314-60100

1100/1200 Series Diode Array Detector (DAD)/Multiple Wavelength Detector(MWD)Long life HiS Deuterium lamp(8-pin) with RFID tag

For G4212A 5190-0917

Long life Deuterium lamp with RFID tag For G1315D/E and G1365D/E 2140-0820

Long life Deuterium lamp For G1315A/B/C and G1365A/B/C 2140-0813

Long life Deuterium lamp For G1315A/B/C and G1365A/B/C 5181-1530

Deuterium lamp For G1315A/B/C and G1365A/B/C 2140-0590

Tungsten lamp For G1315A/B/C/D/E andG1365A/B/C/D/E

G1103-60001



Flow Cell and Repair Kits for VWD

Description Use With Comments Part No.Repair KitPart No.

RepairProcedure*

Standard flow cell, RFID G1314DE 10 mm, 14 μL, 40 bar G1314-60186 G1314-65061 B

Standard "D" type flow cell G1314A/B/C 10 mm, 14 μL, 40 bar G1314-60086

Semi-micro flow cell, RFID G1314D/E 6 mm, 5 μL, 40 bar G1314-60183 G1315-68713 A

Semi-micro flow cell G1314A/B/C 6 mm, 5 μL, 40 bar G1314-60083

Micro flow cell, 3 mm, RFID G1314D/E 2 μL, 120 bar G1314-60187 G1315-68713 A

Micro flow cell, 3 mm G1314A/B/C 2 μL, 120 bar G1314-60087

Micro flow cell, 5 mm G1314A/B/C 1 μL, 40 bar G1314-60081

High pressure flow cell, RFID G1314D/E 10 mm, 14 μL, 400 bar G1314-60182 G1314-65054 B

High pressure flow cell G1314A/B/C 10 mm, 14 μL, 400 bar G1314-60082*See page 57 for repair procedures

Capillaries for VWD Flow CellUse With Inlet Capillary Part No. Outlet Capillary Part No.

Standard flow cell, RFIDStandard "D" type flow cell

Inlet capillary, 600 mm, 0.17 mm ID 5062-8522 Waste capillary, PEEK, 0.25 mm ID 5062-8535

Fingertight fitting 0100-1516

Semi-micro flow cell, RFIDSemi-micro flow cell

Inlet capillary, 400 mm, 0.12 mm ID 5021-1823 Waste capillary, PEEK, 0.25 mm ID 5062-8535

Fingertight fitting 0100-1516

Micro flow cell, 3 mm, RFIDMicro flow cell, 3 mmMicro flow cell, 5 mm

Inlet capillary, 310 mm, 0.12 mm ID G1314-87301 Outlet capillary, 120 mm, 0.17mm ID G1314-87302

Inlet capillary, 400 mm, 0.12 mm ID 5021-1823

High pressure flow cell, RFID Inlet capillary, 380 mm, 0.17 mm ID G1315-87311High pressure flow cell



Flow Cell and Repair Kits for DAD/MWD

Description Use With Comments Part No.Repair KitPart No.

RepairProcedure*

Standard flow cell, RFID G1315C/D, G1365C/D 10 mm, 13 μL, 120 bar G1315-60022 G1315-68712 A

Standard flow cell G1315A/B, G1365A/B 10 mm, 13 μL, 120 bar G1315-60012

Semi-micro flow cell, RFID G1315C/D, G1365C/D 6 mm, 5 μL, 120 bar G1315-60025 G1315-68713 A

Semi-micro flow cell G1315A/B, G1365A/B 6 mm, 5 μL, 120 bar G1315-60011

Micro flow cell, RFID G1315C/D, G1365C/D 3 mm, 2 μL, 120 bar G1315-60024 G1315-68713 A

Micro high-pressure flow cell G1315A/B, G1365A/B 6 mm, 1.7 μL, 400 bar G1315-60015 N/A

Preparative flow cell G1315A/B, G1365A/B 3 mm, 120 bar, stainless steel G1315-60016 G1315-68712 A

Max-light cartridge cell G4212A Infinity DAD 10 mm, 1.0 μL G4212-60008 N/A

Max-light cartridge test cell Must be used to performdetector build-IN tests

Must be used to performdetector build-IN tests

G4212-60011 N/A

*See page 57 for repair procedures

Capillaries for DAD/MWD Flow CellFlow Cell Description Part No. Inlet Capillary Part No. Outlet Capillary Part No.

Standard flow cellwith RFID tag

G1315-60022 Inlet capillary with heatexchanger, 0.17 mmID, 590 mm long

G1315-87321 Outlet capillary, 0.17 mm ID, 200 mm long G1315-87302

Standard flow cell G1315-60012

Semi-micro flow cellwith RFID tag

G1315-60025 DAD heat exchangercapillary, 0.17 mm ID,310 mm long


Outlet capillary, 0.17 mm ID, 200 mm long G1315-87302

Semi-micro flow cell G1315-60011 Outlet capillary, 0.12 mm ID, 200 mm long G1315-87306


Micro flow cellwith RFID tag

G1315-60024 DAD heat exchangercapillary, 0.12 mm ID,310 mm long



Micro high-pressureflow cell

G1315-60015 Inlet capillary with heatexchanger, 0.12 mmID, 290 mm long




500 nL flow cell and replacement parts

80 nL and 500 nL Flow Cell SuppliesDescription Unit Part No.

Fitting screw 10/pk 5063-6593

Double winged nuts and 1/32 in. ferrules 10/pk 5065-4422

1/32 in. ferrule and SS lock ring, lite touch 10/pk 5063-6592

Union adjustment tool 2/pk 5022-2146

Universal ZDV union, stainless steel, no fittings 2/pk 5022-2184

Torque wrench adapter G1315-45003

Open end wrench, 4 mm 8710-1534

500 nL Flow Cell and Replacement PartsDescription Comments Part No.

500 nL flow cell Contains quartz flow cell with10 mm path length and 500 nLvolume and connecting capillaries,max 50 bar pressure

G1315-68724

Sealing kit Includes torque adapter, 2 cell sealassemblies, 5 litetouch front andback ferrules

G1315-68715

Quartz cell body, 10 mm G1315-80001

Cell seal assembly, 500 nL G1315-87101

Fused silica/PEEK capillary,100 μm ID, 30 cm long

Inlet G1315-87333


Inlet G1315-87323


Outlet G1315-87338


Outlet G1315-87328



80 nL Flow Cell and Replacement PartsDescription Comments Part No.

80 nL flow cell Contains quartz flow cell with 6 mm pathlength and 80 nL volume and connectingcapillaries, max 50 bar pressure

G1315-68716

Sealing kit for 80 nL flow cell Includes torque adapter, 2 cell sealassemblies, 5 litetouch front and backferrules and 5 sleeves for 360 μmOD capillaries

G1315-68725

Quartz cell body, 80 nL, 6 mm pathlength

G1315-80002

Fused silica/PEEK capillary, 50 μm ID,40 cm long

Inlet G1315-87323


Outlet G1315-87328


Inlet G1315-87313


Outlet G1315-87318

Preparative Flow Cells and Replacement PartsDescription Part No.

Prep flow cell, quartz, 0.3 mm, 20 bar G1315-60017

Prep flow cell, quartz, 0.06 mm, 20 bar G1315-60018

PTFE tubing, 0.8 mm ID, 2 m G1315-67301

PTFE tubing, 0.5 mm ID, 0.8 m G1315-67302

Cell housing G1315-27705

1/16 in. finger-tight PEEK fittings, beige 0100-1516

Quartz body, 0.3 mm G1315-80004

Quartz body, 0.06 mm G1315-80003

Prep flow cell stainless steel, 3 mm, 120 bar G1315-60016

Stainless steel connecting capillary, 0.5 mm, 250 mm G1315-87305



Detector Maintenance KitsDescription Comments Part No.

1100/1200 Series Variable Wavelength Detector (VWD)G1314A VW detector accessory kit Includes 1/4 in. waste tubing, 2 hex keys,

2 wrenches, outlet tubing, 1/16 in. PEEKmale fitting

G1314-68705

Standard "D" type flow cell kit Includes 2 windows, 2 gaskets #1,2 gaskets #2

G1314-65061

Semi-micro flow cell kit Includes 2 windows, 4 gaskets:2 standard #1, 1 semi-micro #1,1 semi-micro #2

G1314-65056

Micro flow cell kit Includes 2 windows, 2 gaskets #1,2 gaskets #2

G1314-65052

Cell repair kit, semi-micro Includes window screw kit, 4 mmhexagonal wrench and seal kits

G1315-68713

High-pressure flow cell kit Includes 2 windows, 2 Kapton gasketsand 2 PEEK rings

G1314-65054

1100/1200 Series Diode Array Detector (DAD)/Multiple Wavelength Detector (MWD)Cell repair kit Includes window screw kit, 4 mm

hexagonal wrench and seal kitG1315-68712

Cell repair kit, semi-micro Includes window screw kit, 4 mmhexagonal wrench and seal kits

G1315-68713

Sealing kit for 500 nL flow cell Includes torque adapter, 2 cell sealassemblies, 5 litetouch front andback ferrules

G1315-68715

Sealing kit for 80 nL flow cell Includes torque adapter, 2 cell sealassemblies, 5 litetouch front and backferrules and 5 sleeves for 360 μmOD capillaries

G1315-68725



G4218A 1200 SeriesEvaporating Light Scattering Detector SuppliesDescription Part No.

Standard flow nebulizer G4218-20000

Semi-micro flow nebulizer G4218-20001

Large flow nebulizer G4218-20002

Micro flow nebulizer G4218-20003

RRLC nebulizer G4218-20004

Nebulization chamber, glass G4218-40000

Black plastic nut, 13 mm diameter, glassware G4218-40010

Black plastic nut, 22 mm diameter, glassware G4218-40011

Black exhaust tube, 2.5 m G4218-40110

Bulkhead G4218-40130

Cartridge, 0.01 μm for gas regulator G4218-40150

Pneumatic tube with stainless steel fitting G4218-40220

Drain tube with stainless steel fitting G4218-40100

Gas regulator with 0.01 μm filter and manometer G4218-60100

Seal kit for nebulization chamber G4218-68010

Caffeine standard, 250 μg/mL G4218-85000



G1362A 1100/1200 SeriesRefractive Index Detector (RID) SuppliesDescription Part No.

Tubing kitIncludes 300 mm recycle valve to recycle port, 200 mm recycle valve to wasteport, 120 mm purge valve to recycle valve, 270 mm purge valve to sample cell,170 mm purge valve to reference cell

G1362-68709

Interface tubing kitIncludes 1/8 in. ferrule, 1/3 in. nut, PTFE tubing

G1362-68706

Interface capillary, 400 mm, 0.17 mm ID G1362-87300

Restriction capillary, 0.17 mm ID G1362-87301



Stainless steel front ferrules, 5180-4108

Flow cell for G1321A fluorescence detector, G1321-60005

Back ferrule 1/16 in., 5180-4114

G1321A 1100/1200 Series Fluorescence Detector(FLD) SuppliesDescription Part No.

Xenon flash detector lamp 2140-0600

Flow cell G1321-60005

Cuvette kit, 8 μL, 20 barIncludes tubing, SS fitting, front and back ferrule, PEEK fitting, syringe needleand syringe

G1321-60007

Cut-off filter kit:

389, 408, 450, 500, 550 nm 5061-3327

380, 399, 418, 470, 520 nm 5061-3328

280, 295, 305, 335, 345 nm 5061-3329

Corrugated tubing, polypropylene, 6.5 mm ID, 5 m 5062-2463

PTFE tubing, FEP, 0.7 mm ID, 5 m 5062-2462

1/16 in. finger-tight PEEK fittings, beige, 2/pk 0100-1516

Column connecting capillary with fittings, 380 x 0.17 mm G1315-87311

1/16 in. front ferrule, stainless steel, 10/pk 5180-4108

1/16 in. back ferrule, stainless steel, 10/pk 5180-4114

1/16 in. male fittings, stainless steel, 10/pk 5061-3303

Hex key, 4.0 mm, 10 cm long straight handle 5965-0027

Hex key, 2.5 mm, 10 cm long straight handle 5965-0028

Fluorescence detector calibration sample, 1 g glycogen 5063-6597

Hex key set, 1-5 mm 8710-0641

Open end wrench, 1/4 and 5/16 in. 8710-0510

Glass syringe 9301-1446

Syringe needle 9301-0407



1100/1200 Series Chip LC SuppliesDescription Part No.

Rotor, inner valve, 3 grooves, chip LC G4240-23705

Rotor, outer valve, 5 grooves, chip LC G4240-25206

PEEK fitting, special for chip LC G4240-43200

Fused silica/PEEK capillary, 15 μm, 90 cmNano pump to chip cube

G4240-87300

Fused silica/PEEK capillary, 25 μm, 105 cmMicro well plate sampler to chip cube

G4240-87301

Fused silica/PEEK capillary, 100 μm, 100 cmChip cube to waste

G4240-87302

Fused silica/PEEK capillary, 75 μm, 100 cmSyringe pump to chip cube

G4240-87303

Fused Silica/PEEK capillary, 50 μm, 50 cm G4240-87304

Inline micro filter kit, 0.5 μm, PEEKUse with chip cube LC system

5067-1582

Fitting with 0.5 μm PEEK frit, 10/pk 5067-1584

PEEK fitting for use with 1/32 in. OD, 10/pk 5067-1585


Combined with Agilent's industry-leading LC systems, our single quadrupole, ion trap,triple quadrupole, TOF and Q-TOF LC/MS solutions combine world-class performancewith legendary reliability and ease-of-use.

This section contains essential information to keep your LC mass spectrometer runningat peak performance, including:

• LC/MS maintenance, including routine procedures

• Supplies, chemicals, calibrants, and tools for maintaining your system

• Quiet Cover II for easy movement and maintenance of your rough pumps

LC/MS System Maintenance

A higher level of purity for resultsthat stand up to scrutiny.



LC/MS Maintenance Schedule

Procedure When to Perform

Flush the nebulizer Daily or at the end of each shift to flush traces of samplesand buffers out of the tubing, valves, and nebulizer.

Clean the electrosprayspray chamber

Daily or anytime you suspect carryover contamination fromone sample or analysis to another.

Replace the electrospraynebulizer needle

When the needle is plugged. Common symptoms of a pluggedneedle are increased LC back pressure, off-axis spraying, ordripping from the nebulizer.

Clean the APCI spraychamber

Daily or anytime you suspect carryover contamination from onesample or analysis to another.

Replace the APCInebulizer needle

When the needle is plugged. Common symptoms of a pluggedneedle are increased LC back pressure or off-axis spray fromthe nebulizer.

Clean the multimode source Daily or anytime you suspect carryover contamination from onesample or analysis to another, or when you must access the endcap and capillary cap for cleaning and inspection.

Check calibrant levels Monthly or weekly if you tune the LC/MS frequently.



LC/MS Supplies

Description

6100 SeriesSingle

QuadropoleLC/MS

6200 SeriesTOF LC/MS

6300 SeriesIon TrapLC/MS

6400 SeriesTriple

QuadropoleLC/MS

6500 SeriesAccurate-Mass

Q-TOFLC/MS Part No.

ES nebulizer assemblyUsed for analytical sprayer

] ] ] ] ] G1946-60098

API-ES nebulizer needlereplacement kit

] ] ] ] ] G2427A

APCI nebulizer assembly ] ] ] ] ] G1946-60037

APCI nebulizer needle replacement kit ] ] ] ] ] G2428A

Needle assembly ] ] ] ] G1947-60103

Corona needle APCI ] ] ] ] ] G1947-20029

Capillary cap (G1946A/B/C) ] G1946-20056

Capillary cap(G1946D and G1956A/B)

] ] ] ] ] G1946-20301

Capillary (G1946A/B/C and G1956A) ] ] G1946-80009

Capillary (G1946D, G1956B) ] ] ] ] ] 59987-20040

Capillary (G6140A, G6460A, G6530A) ] G1960-80060

Spring canted coil, 0.25 in. ID,0.53 mm

] ] ] ] ] 1460-2571

1/6 in. tee, low dead volume,stainless steel

] ] 0100-0969




Syringe adapter ] 9301-1291

Syringe pump ] 3162-0178

1/16 in. finger-tight PEEK fittings, beige ] ] ] 0100-1516

Female luer to female 10/32 adapter ] ] ] 0100-2304

PEEK tubing ] ] ] 0890-1915

Gas-tight syringe, PTFE luer lock ] ] ] 5182-9710

High-throughput skimmer, 2 mm ] ] ] G1969-20302

Skimmer 1 (G1946A/B/C) ] G1946-20089

Skimmer 1 (G1946D) ] G1946-20302

Skimmer 1 (G1956A/B) ] G1956-20302

Skimmer 2(G1946A/B/C and G1956A only)

] G1946-20087

HED electron multiplier(G1946A/B/C/D)

] G1946-80019

HED electron multiplier(G6140A, G6460A, G6530A)

] G2571-80103

Detector assembly (G1956A/B) ] G1956-80000

Electron multiplier replacement horn ] ] 05971-80103

Replacement horn and dynode ] ] G2441-80010

Description

6100 SeriesSingle

QuadropoleLC/MS

6200 SeriesTOF

LC/MS


6400 SeriesTriple

QuadropoleLC/MS


Q-TOFLC/MS Part No.

LC/MS Supplies (Continued)



LC/MS Chemicals

Description

6100 SeriesSingle

QuadropoleLC/MS

6200 SeriesTOF

LC/MS


6400 SeriesTriple

QuadropoleLC/MS


Q-TOFLC/MS Part No.

ES/APCI positive ion performancestandard, 5 x 1 mL ampoules

] ] ] ] G2423A

Electrospray LC demo sample(Sulfamix)

] ]* 59987-20033

ESI+APCI LC demo sample ] G1978-85000

ES Negative Ion PerformanceStandard, 5 x 1 mL ampoules

] ] G2424A

APCI Negative Ion PerformanceStandard, 5 x 1 mL ampoules

] G2425A

ES/APCI Positive IonPerformance Standard

] G1946-85004

Multiple-charge compoundperformance evaluation sample(horse heart myoglobin)

] G2426A

Caffeine Standards Kitfor LC/MS OQ/PV

] 8500-6917

ES/APCI positive ion performancestandard, 5 x 1 mL ampoules

] G2423A

ES-TOF reference mix,6 x 2 mL ampoules

] ] G1969-85001

ES-TOF Biopolymer referencestandard kit

] G1969-85003

Flushing solvent ] ] ] ] ] G1969-85026

High purity water, 4 L ] ] ] ] ] 8500-2236

Methyl alcohol, 1 L ] ] ] ] ] 8500-1867

Ammonium formate ] ] ] ] ] G1946-85021

Formic acid, 5 mL ] ] ] ] ] G2453-85060

Acetonitrile, 1 L ] ] ] ] ] G2453-85050*Recommended item for familiarization



LC/MS Calibrant Mix and Source Compatibility Matrix

Source

6100 SeriesSingle

QuadropoleLC/MS

6140 SeriesSingle

QuadropoleLC/MS

6200 SeriesTOF

LC/MS


6400 SeriesTriple Quadropole

LC/MS

6500 SeriesAccurate-MassQ-TOF LC/MS

ESI G2421A G1969-85000 G1969-85000 G2431A G1969-85000 G1969-85000

APCI G2432A G1969-85010 G1969-85010 G2432A G1969-85010** G1969-85010**

APPI G2432A G2432A G1969-85010 G2432A G2432A** G1969-85010**

MMI G2432A G1969-85000 G1969-85020 G2432A G1969-85020 G1969-85020

NanoESI G1969-85000**** G2431A G1969-85000****

HPLC chip cube G1969-85000** G1969-85000**** G2431A* G1969-85000*** G1969-85000*****5X dilution suggested

**No autotune

***ESI positive tune only

****Calibration only

LC/MS Calibrant MixesDescription Part No.

ESI tuning mix, 100 mL G2421A

APCI/APPI calibrant solution, 100 mL G2432A

ESI tuning mix for ion trap, 100 mL G2431A

ES-TOF tuning mix, 100 mL G1969-85000

APCI-L low concentration tuning mix, 100 mL G1969-85010

MMI-L low concentration tuning mix, 100 mL G1969-85020



LC/MS Pump SuppliesDescription Part No.

Oil mist filter kit for E2M18 3162-1056

Oil return kit 3162-1057

Foreline pump oil, Inland 45, 1 L 6040-0834

Rotary pump oil, 4 L 6040-0798

Foreline exhaust adapter 59980-20134

Hose clamp 1400-0563

Oil mist filter element for E2M18 1535-4970

KF25 clamp, stainless steel 0100-0549

KF25 coseal (inside clamp) 0100-1597

Exhaust tubing 0890-1727

Pump oil drip pan G1946-00034



*These parts are common to all LC MSD systems

Nitrogen gas generator, 5183-2003

LC/MS Common Supplies*Description Part No.

Common Parts

Filter element, 5 μm, 5/pk 0100-2051

Rotor seal, Tefzel, pH 0 to 14 0100-1854

Rotor seal, Vespel, pH 0 to 10 0100-1855

Inlet filter assembly G1946-60180

SSV long drain tubing assembly G1969-60086

Spring canted coil 1460-2571

Cleaning Supplies

Abrasive mesh, 4000 grit 8660-0827

Capillary cleaning wire for dip tube G1946-80054

Cleaning powder, dielectric capillary, Alconox 5190-1401

Cloths, lint free 05980-60051

Cotton swabs, 100/pk 5080-5400

Gas Purifiers and Generators

Big hydrocarbon trap, 1/4 in. fittings BHT-4

Big universal trap, 1/8 in. fittings, nitrogen RMSN-2

Nitrogen gas generator 5183-2003

Nitrogen gas generator with oxygen monitor, 120/220V 5183-2004

Maintenance kit for LC MSD nitrogen generator 5183-2014




(Continued)

Tools

G1946A tool kit G1946-60157

Nebulizer adjustment fixture G1946-20215

Nebulizer 25X magnifier G1946-80049

Plastic tubing cutter 8710-1930

Screwdriver, Torx T15 8710-1622

Screwdriver, Torx T20 8710-1615

Open end wrench, 1/4 and 5/16 in. 8710-0510

Wrench, 1/2 and 7/16 in. 8710-0806

Needle nose pliers, pointed serrated jaws 8710-0004

3 mm wrench for nebulizer needle adjustment 8710-2699

Maintenance Kit

LC/MS Preventive Maintenance kitIncludes vacuum pump oil, oil element, big hydrocarbon trap, 5 μm filter,rotor seal and canted coil spring

5190-1443

*These parts are common to all LC MSD systems

Description Part No.

LC/MS Common Supplies*



Quiet Cover

Agilent has a solution to the frequent maintenance and inherent noise of LC/MS rough pumps.The Quiet Cover II was designed for easy movement, maintenance, and better living withrough pumps used with Agilent and other LC/MS systems.

• Locking castors to move heavy pump for maintenance

• No tools necessary to remove sectioned cover for easy access to pump

• Built in Lift and Tilt lever raises end of pump to drain oil

• Removable drip pan with well and hand holds to collect and transport oil

• Sound absorbing cabinet with resistant foam insulation to reduce pump noise

• Pump mounted on cushioned grommets to minimize vibration

• 2 Integrated fans maintain temperature inside cover

• LEDs and audible alarm if temperature exceeds 35° C limit

• Maximum ambient temperature of 35° C when airflow is neither restricted nor recycled

• Standard one-year warranty. Installation and familiarization included with new LC/MS orders

The Quiet Cover II is compatible with these Agilent LC/MS Systems:

• 6100 Single Quads: G6110AA, G6120AA, G6130AA, G6140AA

• 6300 Traps: G2440DA, G2451AA, G4533AA, G2474SS

• 6410 QQQ: G6410AA

• 6210 TOF: G3250AA, G3252A

• 6510AA Q-TOF: G6510AA

Or any analytical system using BOC Edwards pumps (lbs/kg): E2M28, E2M18, E1M18.

Quiet Cover II for Agilent LC/MS Systems


Quiet Cover II for Agilent LC/MS Systems12.5 W x 17.3 H x 33.5 L

G3199B

Quiet Cover


While supplies and accessories are the least expensive components of your LC system, theycan contribute to problems such as injector damage, ghost peaks, and analyte degradation.

That is why Agilent supplies are engineered with the same reliability and reproducibility youexpect from Agilent instruments. And they are precisely designed to work with your 1100,1120, 1200 or 1290 Series HPLC System.

The following pages feature a wide selection of Agilent-engineered supplies, including:

• Connection capillaries

• Fittings and unions

• LC tubing

• In-line and solvent filters

• Frits and adapters

• Sample filtration supplies

These supplies are also designed for fast, easy replacement, so you can get your systemrunning again quickly.

General Supplies

They may be small, but they have a BIG effect on yourproductivity and results.


General Supplies

• Made of flexible stainless steel (0.6 mm OD) with 1/16 in. OD tubing at both ends to accept standard fittings

• Pre-swaged fittings are assembled according to Swagelok specifications


Capillaries for 1100 and 1200 LC SystemsFrom To ID (mm) Length (mm) Material* Color Code Fittings/Connection Part No.

Pump Autosampler 0.17 600 SS Green Pre-swaged G1312-67305

0.25 130 SS Blue Pre-swaged 01090-87308

0.25 320 SS Blue Pre-swaged 79835-87638

0.17 500 SS Green Non-swaged G1328-87600

Manual valve 0.17 900 SS Green 1 end pre-swaged G1329-87300

Universal 0.25 700 SS Blue 1 end pre-swaged 01018-67305

1 multi assembly 0.6 173 SS Male to male G1361-67300

1 multi assembly 0.6 175 SS Male to male G1361-67301

Restriction capillary SS A/A G1312-67302

SSV/MCGV AIV Pre-swaged PTFE Pre-swaged G1311-67304

OBV Piston 2 0.6 230 SS Pre-swaged G1312-67300

Mixing capillary Damper 0.17 495 SS Green Pre-swaged G1312-67304

Damper Mixer 0.25 130 SS Blue Pre-swaged 01090-87308

Damper Purge valve SS A/A G1312-67301

Filter EMPV SS A/A G1375-87400

Needle seat Injection valve SS -C G1329-87101

EMPV Next module 0.6 400 SS Male to male G1361-67302

EMPV Multi assembly 0.5 160 SS Male to male G1361-67303

Union EMPV2 0.6 111 SS Male to female G1361-67304

Union Mixer 0.6 40 SS Male to female G1361-67305

EMPV1 Union 367 0.6 SS Male to female G1361-67306


General Supplies

Autosampler Column 0.12 180 SS Red 1 end pre-swaged G1313-87304

0.12 280 SS Red 1 end pre-swaged 01090-87610

0.12 105 SS Red 1 end pre-swaged 01090-87611

0.17 180 SS Green 1 end pre-swaged G1313-87305

0.17 280 SS Green 1 end pre-swaged 01090-87304



0.5 600 SS G2260-87300

VWD Waste 0.25 48 PEEK Wastecapillary,

PEEK,0.25 mm ID

Without fittings 5062-8535

Injector valve Waste 0.25 120 SS Blue G1377-87301Injector valve Prep head 0.5 160 SS G2258-87301

Description ID (mm) Length (mm) Material* Color Code Fittings/Connection Part No.

Stainless steel capillary 0.12 210 SS Red Pre-swaged G1316-87328

Stainless steel capillary 0.17 250 SS Green Pre-swaged G1367-87304

Stainless steel capillary 0.12 250 SS Red 1 end pre-swaged G1373-87300*SS = stainless steel

Manual valve Column 0.17 500 SS Green Non-swaged G1328-87600

Column compartment Column 0.12 70 SS Red 1 end pre-swaged G1316-87303

Detector Waste 0.17 90 SS Green Non-swaged G1316-87300

Column DAD 0.12 150 SS Red Pre-swaged G1315-87312

0.17 380 SS Green Pre-swaged G1315-87311

Column VWD 0.18 40 PEEK Inlet capillary,600 mm,

0.17 mm ID

With fittings 5062-8522

0.12 105 SS Red Without fittings 5021-1820




0.12 70 SS Red 1 end pre-swaged G1316-87303

0.17 105 SS Green Without fittings 5021-1816




0.17 90 SS Green With fittings G1316-87300

From To ID (mm) Length (mm) Material* Color Code Fittings/Connection Part No.

Capillaries for 1100 and 1200 LC Systems (Continued)


General Supplies

1200 Rapid Resolution Stainless Steel Connection CapillariesFrom To ID (mm) Length (mm) Connection Fittings Part No.

Pump Autosampler 0.17 400 Male to male Pre-swaged G1312-87303

Cooled autosampler 0.17 700 Male to male Pre-swaged G1312-87304

Damper Pressure sensor 0.17 150 Male to male Pre-swaged G1312-87305

DamperMixer

MixerOutlet valve

0.17 105 Male to male Pre-swaged G1312-87306

Column Flow cell 0.17 150 Male to female Non-swaged G1315-87303

Cell out MS 0.12 500 Male to male 1 end pre-swaged G1315-87307

DAD heat exchanger capillary 0.17 310 Male to male 1 end pre-swaged G1315-87319


Valve Valve 0.12 100 Male to male Non-swaged G1316-27301*

Micro valve Regeneration pump 0.12 130 Male to female Non-swaged G1316-87304*

WPS Micro valve 0.12 340 Male to male 1 end pre-swaged G1316-87305*

Micro valve Heat exchanger 0.12 75 Male to male Non-swaged G1316-87306*

TCCVWD

MS 0.12 500 Male to male 1 end pre-swaged G1316-87309

Column DAD cell 0.12 50 Male to female Non-swaged G1316-87312

0.12 70 Male to female Non-swaged G1316-87313




WPS TCC 0.12 170 Male to male Non-swaged G1316-87316

0.12 210 Male to male Non-swaged G1316-87317*0.8 mm OD stainless steel capillaries – use 0.8 mm ID fittings


General Supplies

Cooled WPS TCC 0.12 300 Male to male Non-swaged G1316-87318

0.12 340 Male to male Non-swaged G1316-87319

Column Cooler (50-150 mm column) 0.17 105 Male to male Non-swaged G1316-87321

Cooler (20-30 mm column) 0.17 170 Male to male Non-swaged G1316-87323

Micro valve Detector 0.12 75 Male to female Non-swaged G1316-87326*

Column DAD cell 0.12 170 Male to female Non-swaged G1316-87327

WPS 10 port valve 0.12 210 Male to male Pre-swaged G1316-87328*

10 port valve Column 0.12 100 Male to male Non-swaged G1367-87303*

WPS TCC 0.17 250 Male to male Pre-swaged G1367-87304

Description ID (mm) Length (mm) Connection Fittings Part No.



Absorber capillary, 500 μL 0.17 1770 Male to male Pre-swaged G1312-87300

Calibration capillary assembly G1312-67500

Stainless steel restriction capillary, T-piece to pressure sensor 0.17 G1312-87301

Column connecting capillary with fittings 0.17 90 Male to male Non-swaged G1316-87300

Flexible capillary 0.17 280 Without fittings 5021-1818

Stainless steel capillary 0.17 230 Male to male Pre-swaged 5067-1570

Stainless steel capillary 0.17 320 Male to male Pre-swaged 5067-1571

Stainless steel capillary 0.17 150 Male to female Pre-swaged 5067-1572*0.8 mm OD stainless steel capillaries – use 0.8 mm ID fittings

From To ID (mm) Length (mm) Connection Fittings Part No.

1200 Rapid Resolution Stainless Steel Connection Capillaries (Continued)


General Supplies

1290 Infinity LC Stainless SteelConnection CapillariesFrom To ID (mm) Length (mm) Fittings Part No.

Pump Autosampler 0.17 300 Pre-swaged 5067-4657

Pump ThermostattedAutosampler

0.17 450 Pre-swaged 5067-4658

Autosampler TCC 0.12 340 Non-swaged 5067-4659

TCC DAD 0.12 220 Non-swaged 5067-4660

1290 LC System CTCAutosampler

0.17 600 Pre-swaged 5067-4670

CTC Autosampler Column 0.12 600 Non-swaged 5067-4669

Autosampler Valve 0.12 340 Pre-swaged 5067-4647

Second pump Valve 0.17 700 Pre-swaged 5067-4648

Valve HX1-HX2 0.12 90 Pre-swaged 5067-4649

Column Valve (short) 0.12 150 Non-swaged 5067-4650

Column Valve (long) 0.12 280 Non-swaged 5067-4651

Valve Valve 0.12 120 Pre-swaged 5067-4652

Valve Detector 0.12 200 Pre-swaged 5067-4653

Filter Purge valve 0.25 250 Pre-swaged 5067-4655

Pressure sensor Purge valve 0.25 80 Pre-swaged 5067-4656

Mixer Purge valve 0.17 140 Pre-swaged G4220-87000

Valve Meteringdevice

0.17 160 Pre-swaged G4226-60301


General Supplies

PEEK Coated Fused Silica Capillariesfor use with 20 μL/min Flow RangeFrom To ID (μm) Length (mm) Fitting Part No.

EMPV Flow sensor 50 220 B/B G1375-87301

Flow sensor Injection valve 50 550 B/C G1375-87310

Injection valve Metering device 50 200 B/C G1375-87302

Metering device Needle 100 110 B/B G1375-87303

Injection valve Column 50 500 C/D G1375-87304

Column Detector 50 400 D/E G1315-68703

Detector Waste 75 700 E/- G1315-68708

μ-switching valve Column 50 280 C/D G1375-87309

PEEK Coated Fused Silica Capillariesfor use with 100 μL/min Flow RangeFrom To ID (μm) Length (mm) Fitting Part No.



Injection valve Metering device 100 200 B/C G1375-87312

Metering device Needle 100 110 B/B G1375-87303

Injection valve Column 75 500 C/D G1375-87311

Column Detector 75 400 D/E G1375-87308

Detector Waste 75 700 E/- G1315-68708

μ-switching valve Column 50 280 C/D G1375-87309


General Supplies

PEEK Coated Fused Silica Capillariesfor Nano LCFrom To ID (μm) Length (mm) Fitting Part No.

Switching valve Column 25 100 C/D G1375-87320



Injection valve Flow sensor 25 550 C/D G1375-87323

Switching valve Column



Injection valve Injector seat or2nd pump

75 650 C/D G1375-87327

Replacement Fittings and Ferrulesfor Capillary and Nano Flow SystemGraphic Description Type Part No.

1/16 in. stainless steel fittings,front and back ferrules, 10/pk

A 5062-2418

1/16 in. stainless steel fittings, male,4 mm, 10/pk

B 5063-6593

1/32 in. ferrule and stainless steellock ring, 10/pk

B 5065-4423

6 fittings, 2 plugs, PEEK for μ-valves C 5065-4410

Double winged PEEK nuts and 1/32 in.ferrules, 10/pk

D 5065-4422

PEEK fitting, long for 1/32 in. OD capillary D 5022-6536


General Supplies

Stainless steel fittings, 5062-2418

Stainless steel long fittings, 5065-4454

PEEK fittings, 5063-6591

PEEK RheFlex fittings, 0100-1631

PEEK long fittings, 5062-8541

Finger-tight PEEK fittings, 5065-4426

ChromTrac identifiers, 0350-1402

Fittings for 1/16 in. OD CapillariesDescription Part No.

1/16 in. stainless steel fittings, front and back ferrules, 10/pk 5062-2418

1/16 in. stainless steel long fittings, front and back ferrules, 10/pk 5065-4454

1/16 in. extra long stainless steel fittings and ferrules, 10/pk 5065-9967

1/16 in. male fittings, stainless steel, 10/pk 5061-3303

1/16 in. front ferrule, stainless steel, 10/pk 5180-4108

1/16 in. back ferrule, stainless steel, 10/pk 5180-4114



1/16 in. finger-tight PEEK long fittings, beige, 10/pk 5062-8541

1/16 in. finger-tight PEEK fittings, 10/pk2 each: yellow, blue, black, green, red

5065-4426

1/16 in. double winged 2 piece fitting, 10/pk 5042-6500

PEEK RheFlex 2-piece fittings, 5/pk 0100-1631

PEEK RheFlex 2-piece fittings, colored, 10/pk 0100-2175

ChromTrac identifiers, 20/pk2 each: black, green, white; 4 each: yellow, blue, red

0350-1402

1/16 in. blanking nut, stainless steel 01080-83202

Stainless steel hex nut fitting, PEEK ferrule, max 600 bar, 6/pk 5067-1540

1/16 in. PEEK ferrule, max 600 bar, 6/pk, for use with 5067-1540 fitting 5067-1547

1/16 in. plastic fitting (plug) 0100-1259

1/16 in. finger-tight polyketone fittings, max pressure 600 bar, 10/pk 5042-8957

0.8 mm ID stainless steel ferrules, 6/pk 5067-1557

0.8 mm ID stainless steel fittings, M4/4 mm, 6/pk 5067-1558


General Supplies

Micro T-connector, PEEK, 5042-8519

ZDV Union with Fittings, 0100-0900

High Flow Union, 5022-2133

UnionsDescription Use With Part No.

True ZDV union, no fittings Nano LC 5022-2145

Universal ZDV union, stainless steel, no fittings Capillary/Nano/Standard LC 5022-2184

ZDV union, with fittings Standard LC 0100-0900

ZDV union, PEEK with fittings Bio applications 0100-2441

High flow union, no fittings Prep LC 5022-2133

PEEK adapter 1/4-28 to 10-32 0100-1847

Adapter, PEEK int. 1/4-28 to ext. 10-32 0100-2298

Barbed Y-Connector PP for 3/16 in. ID tube, 10/pk 5065-9971

Adapter, female to female 1/4-28 5042-8517

Adapter, male luer to female 1/4-28 5042-8518

T-connector, PEEK, swept volume 0.57 μL For 1/16 in. OD tubing 5022-2144

Micro T-connector, PEEK, swept vol 29 nL,with 1/32 in. ID fittings

5042-8519


General Supplies

Flexible Stainless Steel CapillariesWithout FittingsID (mm) Length (mm) Color Code Volume (μL) Part No.

0.12 105 Red 1.2 5021-1820

150 Red 1.7 5021-1821

200 Red 2.3 5065-9935

280 Red 3.2 5021-1822

400 Red 4.5 5021-1823

500 Red 5.6 5065-9964

0.17 105 Green 2.4 5021-1816

150 Green 3.4 5021-1817

200 Green 4.6 5065-9931

280 Green 6.4 5021-1818

400 Green 9.1 5021-1819

600 Green 13.6 5065-9933

700 Green 15.9 5065-9932

900 Green 20.5 5065-9963

0.25 250 Blue 12.3 5065-9979

280 Blue 13.8 5022-6508

320 Blue 15.8 5065-9980

800 Blue 39.3 5065-9930

0.50 105 None 20.6 5065-9927

150 None 29.5 5022-6509

280 None 55 5022-6510

800 None 157 5065-9926

PEEK TubingID (mm) Length (m) Color Code Part No.

0.50 1.5 Orange 0890-1761

0.25 1.5 Blue 0890-1762

0.25 5 Blue 5042-6463

0.18 1.5 Yellow 0890-1763

0.18 5 Yellow 5042-6462

0.13 1.5 Red 0890-1915

0.13 5 Red 5042-6461


General Supplies

Other TubingDescription Comments Length (m) ID (mm) OD (mm) Part No.

PTFE tubing, FEP, primary use for valve solutions 5 0.7 1.6 5062-2462

PTFE solvent tubing, primary use for flow pathfrom solvent bottle to degasser, to pump

5 1.5 3.1 5062-2483

Corrugated tubing, polypropylene 5 6.5 5062-2463

Silicone tubing 5 1 3 5065-9978

Clamps and micro clamps, 10/pk 5065-9976

Barbed Y-Connector PPfor 3/16 in. ID tube, 10/pk

5065-9971

Front seat tube, SS For G2258A 1100/1200Series Dual Loop Autosampler

0.1 0.5 G2258-87316

Back seat tube, SS 0.12 0.5 G2258-87315

Front seat tube, PTFE 0.1 0.2 G2258-87312

Back seat tube, PTFE 0.12 0.25 G2258-87313

Waste tube 0.15 0.8 G2258-87310

Waste tube 0.1 0.8 G2258-87311

Drawing tube assembly for flush solventwith filter and bottle cap

G2258-87307

Tubing assembly, solvent flush G2258-87314

Waste tube For G1313/27/29A1100/1200 SeriesAutosampler

G1313-87300

Waste tubing, 5 m, 6.5 mm ID,corrugated polypropylene

5 6.5 5062-2463

Waste tube, FEP For G1387A 1100/1200Series Micro Autosampler

0.8 1.6 G1375-87326


General Supplies

Capillary and Fittings KitsDescription Contents Part No.

SS flexiblecapillary tubing kit

Includes 0.12 mm ID capillaries:3 each 105 mm long, 1 each 150 mm long,1 each 280 mm long, plus fittings

5061-3304

SS flexiblecapillary tubing kit

Includes pre-swaged 0.12 mm ID capillaries:3 each 105 mm long, 1 each 280 mm long,1 each 35 mm long

5061-3315

Capillary/fittingstarter kit, 0.17 mm ID

Kit includes:Qty 1 – PEEK capillary 0.18 mm ID, 1.5 mQty 4 – Stainless steel capillary, 105 x 0.17 mmQty 4 – Stainless steel capillary, 150 x 0.17 mmQty 2 – Stainless steel capillary, 200 x 0.17 mmQty 2 – Stainless steel capillary, 280 x 0.17 mmQty 1 – Stainless steel capillary, 400 x 0.17 mmTubing cutter for PEEK capillaries1/16 in. stainless steel fittings, 10/pk1/16 in. PEEK fittings, color, 10/pk1/16 in. PEEK fittings, 10/pkQty 3 – ZDV union, stainless steelRheotoolCybertool

5065-9939

AccessoriesDescription Part No.

Plastic tubing cutter 8710-1930

Blades for plastic tubing cutter, 5/pk 8710-1931

Fitting screws, SS, 10-32, 4 mm, 5/pk 5065-9948

PEEK ferrule and stainless steel ring for 2 mm tube, 5/pk 5065-9950

Union, PEEK for 1/8 in. OD tubing 0100-2410

Waste adapter, 1200 Series autosamplers, gray G1313-43216



General Supplies

Capillary/fitting starter kit,0.12 mm ID

Kit includes:PEEK capillary, 0.13 mm ID, 1.5 mQty 4 – Stainless steel capillary, 105 x 0.12 mmQty 4 – Stainless steel capillary, 150 x 0.12 mmQty 2 – Stainless steel capillary, 200 x 0.12 mmQty 2 – Stainless steel capillary, 280 x 0.12 mmStainless steel capillary, 400 x 0.12 mmTubing cutter for PEEK capillaries1/16 in. stainless steel fittings, 10/pk1/16 in. PEEK fittings, color, 10/pk1/16 in. PEEK fittings, 10/pkQty 3 – ZDV fittingRheotoolCybertool

5065-9937

Capillary/fittingstarter kit for1100 Capillary LC System

Kit includes:Qty 2 – Fused silica/PEEK capillary, 50 μm, 55 cmQty 1 – Fused silica/PEEK capillary, 50 μm, 20 cmQty 1 – Fused silica/PEEK capillary, 100 μm, 110 cmQty 2 – Fused silica/PEEK capillary, 50 μm, 50 cmQty 2 – Fused silica/PEEK capillary, 50 μm, 40 cmQty 4 – 4 mm stainless steel fitting, male 10-32Qty 4 – 1/32 in. PEEK ferrule and stainless steel

lock ringQty 4 – PEEK fittings for μ-valvesQty 4 – Double winged PEEK nuts and 1/32 in. ferrulesQty 1 – Cybertool

5065-9938

Rapid ResolutionHigh Throughputcapillary kit

Kit includes:Qty 5 – Capillaries to optimize 1100 Series LCfor use with RRHT columns

5065-9947

1200 capillary kitfor 0.12 mm ID

Includes capillaries to optimize 1200 LCfor low dispersion operation

G1316-68716

Description Contents Part No.

Capillary and Fittings Kits (Continued)


General Supplies

HPLC In-Line Filters

DescriptionFrit Porosity

(μm)Frit Inlet ID

(mm) Comments Part No. Replacement Frits

RRLC in-line filter4.6 mm, 0.2 μm pore size filter, connecting capillary,max 600 bar

0.2 4.6 max 600 bar 5067-1553 5067-1562, 10/pk

RRLC in-line filter2.1 mm, 0.2 μm pore size filter, connecting capillary,max 600 bar

0.2 2.1 max 600 bar 5067-1551 5067-1555, 10/pk

Low dispersion in-line filterIncludes two frits, 2.1 mm, 2 μm pore size;filter holder with inserts; 60 x 0.12 mmconnecting capillary

20.5

2.1 < 1 mL/min 01090-68702 280959-904, 10/pk280959-907, 10/pk

Universal in-line filterIncludes two frits, 4.8 mm, 2 μm pore size;filter holder with inserts; 130 x 0.25 mmconnecting capillary

2 4.8 1-5 mL/min 01090-68703 01090-27609, 2/pk

Semi prep filter 0.5 12.7 1-5 mL/min 5064-8273 5022-2185

High pressure semi prep filter 10 19 5-10 mL/min 5022-2165 5022-2166, 10/pk

Prep filter 10 10-100 mL/min 5065-4500 5065-9901Replacement Glass Cartridge

In-line filter for G1311A Recommended whenhigh salt concentrations are used

G1311-60006

1290 in-line filter 0.3 2.0 1200 bar 5067-4638 5023-0271, 5/pk


General Supplies

Frits and AdaptersDescription Part No.

Solvent mixer, 1100 Series G1312-87330

Solvent mixer, short, 200 μL 5067-1565

Frit adapter, PTFE, for 4.7 mm OD tubing G1361-23205

Frit adapter, PTFE, for 1/8 in. OD tubingFor G2258A Dual Loop Autosampler

G2258-23201

O-ring, Viton, 30 mm 0905-1516

Stainless steel filter assembly with PEEK ring, 2 μm pore size 5022-2192

Solvent Filters

Description Recommended Use Part No. Frit Adapter Part No.Frit InletID (mm)

Frit InletID (mm)


Analytical scale, micro scale 5041-2168 Frit adapter, PTFE, 3 mm, 4/pk 5062-8517 5 3.2


Preparative LC 3150-0944 Frit adapter, 4 mm, PTFE G1361-23204 7 4


For G2258ADual Loop Autosampler

3150-0944 Frit adapter,PTFE for 1/8 in. OD tubing

G2258-23201 7 3.2

Glass filter, 5041-2168


General Supplies

Syringe Filters, Membrane Econofilters, 200/pkDescription Diameter (mm) Pore Size (μm) Part No.

Regenerated cellulose 25 0.2 5185-5830

Regenerated cellulose 25 0.45 5185-5831

Nylon 25 0.2 5185-5832

Nylon 25 0.45 5185-5833

PTFE 25 0.2 5185-5834

PTFE 25 0.45 5185-5835

Mini-UniPrep Syringeless Filters, 100/pk


0.45 μm PTFEFor aggressive samples

5190-1415

0.45 μm NylonFor aqueous and organic samples, pH 3-10

5190-1416

0.45 μm PPFor solvent based samples, low water breakthrough values

5190-1417

0.45 μm regenerated celluloseFor aqueous or organic solvents, very low non-specific protein binding membrane

5190-1418

0.20 μm PTFEFor aggressive samples

5190-1419

0.20 μm NylonFor aqueous and organic samples, pH 3-10

5190-1420

0.20 μm PPFor solvent-based samples, low water breakthrough values

5190-1421

0.20 μm regenerated celluloseFor aqueous or organic solvents, very low non-specific protein binding membrane

5190-1422

Membrane Econofilters, 5185-5830

Sample Filtration

For more information, including chemical compatibilities,visit www.agilent.com/chem/miniuniprep

Mini-UniPrep Syringeless Filters


General Supplies

Solvent Filters/Degassers


HPLC solvent filter/degasser assembly 3150-0577

Replacement Parts for 3150-0577

Glass funnel, 250 mL 5188-2743

PTFE coated sieve 5188-2744

PTFE seal 5188-2745

Funnel base, glass 5188-2746

Filter Membranes

Regenerated Cellulose filter membranesDiameter 47 mm, pore size 0.45 μm, 100/pk

3150-0576

Nylon filter membranesDiameter 47 mm, pore size 0.45 μm, 100/pk

9301-0895

PTFE filter membranesDiameter 47 mm, pore size 0.45 μm, 10/pk

3150-0509

The latest technology and newest developmentsare just a click away with free e-Seminarsfrom Agilent

With different series and topics for troubleshooting, maintenance, informatics, compliance,and industry applications, Agilent e-Seminars are the easy way to get the latest informationwithout the hassle or expense of travel. These free 60 to 90 minute online seminars areoffered continuously:

• Choose from live or recorded events to best fit your schedule

• Participate wherever you are – from the office, home, or hotel

• Ask Agilent experts and industry leaders questions during live Q&A sessions

For more information and to see a complete listing of availablesessions, visit www.agilent.com/chem/eseminars

Agilent e-Seminars

Information, descriptions, and specificationsin this publication are subject to change without notice.

© Agilent Technologies, Inc. 2009Printed in the USA November 9, 20095990-4957EN

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Maintaining

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