MAKING NEW BODY PARTS

BIOT 412, 2011 student

2/6/12

Making a New Bladder• Neurogenic bladder disorder• Cystoplasty; a surgical treatment

• What we did???• Human bladder was engineered!

How???1. Perform bladder biopsy; for urothelial and

muscle cells.2. In vitro culturing and expansion of cells.3. Seeding of cells on a biodegradable bladder-

shaped scaffold.4. New organ was ready after 8 weeks.

Regenerated bladder

Contd…• 5. Anastomosis• 6. Apply fibrin glue.• 7. Omental

wrapping. Anastomosis

Omentum layer

Omental wrapping

Results• After 46 months: • Improved function & capacity of ureter.• Renal function was normal through out the

follow up.• No metabolic complication occured.

• Positives:• No immuno-suppression required!

• Negatives:• No immediate Reconstruction of Vascular supply.• Instant Oxygen and nutrients supply is by

diffusion.• Inflammation before re-angiogenesis.

2/6/12

2. Applying Regenerative Medicine to

Upper Airways

Making a New Trachea

• End stage bronchio-malacia in a 30 year old woman, for example.

• What we did???• Ex- vivo bioengineering of trachea.

How???1. Retrieving of deceased donor

trachea.2. Decellularization.3. Obtaining epithelial cells from

bronchial mucosa.4. Isolation of stem cells from

patient’s bone marrow.5. Differentiation of SCs into

chondrocytes.6. 4 days maturation.7. Human trachea was used as

natural scaffold.8. Seeding with autologous

epithelial cells and chondrocytes.9. Implantation of construct to

replace the diseased one.

Trachea scaffold

Culturing the trachea

Results• After 18 months:• Imaging showed normal architecture.• Patient was doing well.

• Advantage!• Use of natural/ human scaffold.