Magnetic Preparation Kit VI - BIOTECON Diagnostics · 2020-04-25 · Norovirus (Caliciviridae)...

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Applicable for a wide range of matrices: Swabs Sputum Feces Whole blood Serum Tissue and many more Tested at BIOTECON Diagnostics and external labs with: SARS-CoV-2 (Coronaviridae) Norovirus (Caliciviridae) Hepatitis A virus (Picornaviridae) PEDV (Coronaviridae) ASFV (Asfarviridae) Adenovirus (Adenoviridae) PRRSV (Arteriviriae) Influenza type A virus (Orthomyxoviridae) and many more Safe Virus Detection – BIOTECON Diagnostics – simply builds up trust! Integrated: No external lysis or heat treatment Automated: Reduces hands-on time Easy storage and shipping: Most components can be stored at room temperature Sensitive and rapid: Excellent RNA / DNA quality within 45 minutes Reproducible and precise: Minimizes human error Easy: Quick and simple to implement The Magnetic Preparation Kit VI provides reliable purification of viral nucleic acids (RNA and DNA) from a variety of samples. These include nasopharyngeal and throat swabs, sputum, feces, serum and tissue. This newly developed magnetic bead-based isolation kit is designed for automated high-throughput extraction in combination with the RoboPrep ® 32, the KingFisher TM Flex or other automation systems. It greatly speeds up the workflow and decreases manual handling, without the need for external lysis or external heat treatment and centrifugation steps. The kit contains all required reagents, including enzyme, buffer and carrier tRNA for the lysis step, as well as magnetic beads to carry out extraction from complex matrices. Using the flexible RoboPrep ® 32 allows for the immediate processing of various sample numbers as they arrive in the lab (1-32 per unit at a time). Automated Extraction of RNA / DNA from Viruses Magnetic Preparation Kit VI

Transcript of Magnetic Preparation Kit VI - BIOTECON Diagnostics · 2020-04-25 · Norovirus (Caliciviridae)...

Applicable for a wide range of matrices:

� Swabs

� Sputum

� Feces

� Whole blood

� Serum

� Tissue

� and many more

Tested at BIOTECON Diagnostics and external labs with:

� SARS-CoV-2 (Coronaviridae)

� Norovirus (Caliciviridae)

� Hepatitis A virus (Picornaviridae)

� PEDV (Coronaviridae)

� ASFV (Asfarviridae)

� Adenovirus (Adenoviridae)

� PRRSV (Arteriviriae)

� Influenza type A virus (Orthomyxoviridae)

� and many more

Safe Virus Detection – BIOTECON Diagnostics –simply builds up trust!

Integrated: No external lysis or heat

treatment

Automated: Reduces hands-on time

Easy storage and shipping: Most

components can be stored at room

temperature

Sensitive and rapid: Excellent RNA / DNA

quality within 45 minutes

Reproducible and precise: Minimizes

human error

Easy: Quick and simple to implement

The Magnetic Preparation Kit VI provides reliable purification of viral nucleic acids (RNA and DNA) from a variety of samples. These include nasopharyngeal and throat

swabs, sputum, feces, serum and tissue.

This newly developed magnetic bead-based isolation kit is designed for automated high-throughput extraction in combination with the RoboPrep® 32, the KingFisherTM Flex or other automation systems. It greatly speeds up the workflow and decreases manual handling, without the need for external lysis or external heat treatment and centrifugation steps. The kit contains all required reagents, including enzyme, buffer and carrier tRNA for the lysis step, as well as magnetic beads to carry out extraction from complex matrices. Using the flexible RoboPrep® 32 allows for the immediate processing of various sample numbers as they arrive in the lab (1-32 per unit at a time).

Automated Extraction of RNA / DNA from Viruses

Magnetic Preparation Kit VI

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-MP

KV

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V02

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0

BIOTECON Diagnostics GmbH

Hermannswerder 17 14473 Potsdam Germany

Phone: +49 (0)331-2300-200 Fax: +49 (0)331-2300-299

[email protected] www.bc-diagnostics.com

Magnetic Preparation Kit VI- 480 Reactions

Order No. S 400 20.1 L

Workflow

1. Preparation 2. Sample 3. Extraction

Ext

ract

ion

in 4

5 m

in.

� Washing Buffer I and II � Elution Buffer � Lysis Buffer

� Add 200 μl Sample Material � Press Start � Fully Automated Extraction

The above mentioned instruments are registered trademarks of their respective holders.

Validation

MPK VI was validated with RNA and DNA viruses (enveloped and non-enveloped) from a variety of different samples and species. Results showed excellent sensitivity compared to other kits on the market and high linearity as well as reproducibility even at low virus concentrations (Figure 1 and Figure 2).

Available instruments

D 210 32 RoboPrep® 32 (low to medium throughput, semi-automated)D 220 01 KingFisherTM Flex (medium to high throughput, semi-automated)D 250 00 RoboPrep Fusion® (high throughput, full automated)

Graph shows high linearity and reproducibility even at low virus concentrations

Fig. 1: Amplification plot (PRRSV)

Fig. 2: Standard curve (PRRSV)

For research use only, not intended for diagnostic procedures.

Available PCR-Kits

microproof® SARS-CoV-2 Identification Kitmicroproof® SARS-CoV-2 Screening Kit

Additional PCR-Kits from other manufacturers are available, depending on your region.For further information regarding our SARS-CoV-2 detection kits please contact us.