M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli....

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M2D3: Tools for System Engineering 10/18/12 Thursday, October 18, 2012

Transcript of M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli....

Page 1: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

M2D3: Tools for System Engineering10/18/12

Thursday, October 18, 2012

Page 2: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Review of M2D2: Measuring System Performance

Inspect your photograph and document!

Thursday, October 18, 2012

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Page 3: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Part I: Library Screen -- Improving our photography system

Thursday, October 18, 2012

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Page 4: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Part I: Library Screen -- Electroporation

http://www.onemedplace.com/blog/archives/854

MacConkey Media - Agar plates

Thursday, October 18, 2012

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Page 5: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Part 2: Registry of Standard Biological Parts -- Building a protein generating system in e.coli

Web address: http://partsregistry.org

3 Parts --> Protein Production

Thursday, October 18, 2012

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Page 6: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Part 2: Registry of Standard Biological Parts -- Building a protein generating system in e.coli

Thursday, October 18, 2012

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Page 7: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Part 3: Biology as a circuit?

Circuit designed by Steve WassermanThursday, October 18, 2012

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Page 8: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Plans for today:

1. Lab & Hand-in Lab Notebooks

2. Document your picture of Butterstick!

3. Electroporation of mutant library

4. Build a protein production system from standard parts

5. Does Electrical Engineering = Biology?

6. Finish up your TinkerCell simulations (Part 4)

Thursday, October 18, 2012

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Page 9: M2D3: Tools for System Engineering - Amazon S3€¦ · Most parts the Registry function in E. coli. Yeast Yeast are simple eukaryotes. Bacteriophage T7 Bacteriophage T7 an Obligate

Group Color Miller Units in Dark Miller Units in Light

Data!

Thursday, October 18, 2012

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