LS174T p-EGFR MEK 1/2 p-MEK 1/2 AKT actina RAS-T RAS-Act pAKT EGFR GEO HCT15 HCT116 SW480 SW620...
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Transcript of LS174T p-EGFR MEK 1/2 p-MEK 1/2 AKT actina RAS-T RAS-Act pAKT EGFR GEO HCT15 HCT116 SW480 SW620...
LS174T
p-EGFR
MEK 1/2
p-MEK 1/2
AKT
actina
RAS-T
RAS-Act
pAKT
EGFR
GEOHCT15
HCT116
SW48
0
SW62
0
p-MEK 1/2
actina
RAS-Act
p-EGFR
MEK 1/2
AKT
RAS-T
pAKT
GLC82
A549
Calu3
H460
H1299
EGFR
Fig. S1
AB
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.G12D c.35G>A LS174T
p.G12A c.35G>C GEO
p.G12V c.35G>T SW480;SW620
p.G13D c.38G>A HCT116; HCT15
p.G13A c.38G>C
p.G13V c.38G>T
p.G13G c.39C>G
p.G13G c.39C>A
p.G13G c.39C>T
p.G12S c.34G>A A549
p.G12R c.34G>C
p.G12C c.34G>T
p.A59T c.175G>A
p.Q61K c.181C>A
p.Q61E c.181C>T
p.Q61E c.181C>G
p.Q61P c.182A>C
KR
AS
Table S.1A Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.Q61R c.182A>G
p.Q61L c.182A>T
p.Q61H c.183A>C H460
p.Q61H c.182A>T
KR
AS
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.Q61P c.182A>C
p.Q61L c.182A>T
p.Q61R c.182A>G
p.Q61H c.183A>C
p.Q61Q c.183A>G
p.Q61H c.183A>T
p.G61K c.181C>A H1299
p.G61E c.181C>G
p.G13S c.37G>A
p.G13R c.37G>C
p.G13C c.37G>T
p.G12R c.34G>C
p.G12S c.34G>A
NR
AS
Table S.1B Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.K601E c.1801A>G
p.V600E c.1799T>A
p.D594G c.1781A>G
BR
AF
Table S.1C Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE
NUCLEOTIDE MUTATION
EXON CELL LINE
p.H1047R c.3140A>G exon 20 HCT116; GLC82; LS174T
p.H1047L c.3140A>T exon 20
p.P539R c.1616C>G exon 9
p.Q546K c.1636C>A exon 9 A549
p.Q546E c.1636C>G exon 9
p.E81K c.241G>A exon 1
p.R88Q c.263G>A exon 1
p.G106V c.317G>T exon 1
p.N345K c.1035T>A exon 4
p.R93W c.277C>T exon 1
p.S158L c.473C>T exon 2
p.H160N c.478C>A exon 2
p.R38H c.113G>A exon 1
p.E542K c.1624G>A exon 9
p.E542Q c.1624G>C exon 9
p.E545K c.1633G>A exon 9 H460;HCT15
p.E545Q c.1633G>C exon 9
PIK
3CA
Table S.1D Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE
NUCLEOTIDE MUTATION
EXON CELL LINE
p.G118D c.353G>A exon 2
p.G12D c.35G>A exon 1
p.H1047Y c.3139C>T exon 20
p.C420R c.1258T>C exon 7
p.H701P c.2102A>C exon 13
p.K184E c.550A>C exon 2
p.C901F c.2702G>T exon 18
p.M1004I c.3012G>T exon 20
p.G1007R c.3019G>C exon 20
p.G1049R c.3145G>C exon 20
p.R108H c.323G>A exon 1
PIK
3CA
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.R248W c.742C>T
p.Y220C c.659A>GP53
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.F241S c.722T>C
p.Y65C c.194A>G
p.E150Q c.448G>C
p.K62R c.185A>G
p.K267fs*9 c.800delA
PT
EN
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
p.R154W c.460C>T
p.E221 c.661G>T
p.S251N c.752G>A
p.I295fs*23 c.882_882delGME
K 1
/2
Table S.1E Mutation Status of Cell Lines Used in This Study
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
AKT p.A377V c.1130C>T
GENE AMINO ACID CHANGE NUCLEOTIDE MUTATION CELL LINE
EGFR p.E746_A570 c.2236_2250del15
Table S.1F Mutation Status of Cell Lines Used in This Study
KRAS
PI3Kca
BRAF
NRAS
NO YES
RE 2 5
SE 3 1
Χ-test p =
RE SE
No mut 1 2
G12 2 3
G13 1 1
Χ-test p =
NO YES
RE 7 0
SE 3 1
Χ-test p = 1
NO YES
RE 1 4
SE 2 4
Χ-test p =
KRAS mutation NRAS mutationPIK3Ca mutationKRAS (G12and G13) mutationFig. S2
8
1 6
0
0 50
00
0.8466 0.72730.1939
Fig. S7
H460A549LS174THCT15GLC82CALU3HCT116H1299SW620SW480GEO H460A549LS174THCT15GLC82CALU3HCT116H1299SW620SW480GEO
Fig. S3
αs
β γ
GPCRRTK
Ras
Raf-1
MEK1/2
MAPK
ATP cAMP
PKAR
PKAC
AKAP13
RA signaling
Cell proliferationCell differentiation
Cell survivalCell proliferatiom
TAF3
RXRANR1H2
NCOA3
Fig. S4
P
PP P
Grb2
SOSShc
ADCY7