LPS, Microbial toxins - Hycult Biotech · SAP, Mouse, ELISA 1 x 96 det. HK215-01 sCD14, Human,...
Transcript of LPS, Microbial toxins - Hycult Biotech · SAP, Mouse, ELISA 1 x 96 det. HK215-01 sCD14, Human,...
LPS, Microbial toxins
Innate Immunity · Acute phase proteins · Western blot · TLR · Assays · Antimicrobial peptides · Flow
cytometry · LPS, Microbial toxins · Robust · Complement Research · Easy to use · Scavenger receptors ·
Inflammation · Sensitivity · Unique products · Immunohistochemistry · Coagulation molecules · Quality control ·
Cytokines · Chemokines · Antibodies · Cell & Tissue damage · Functional studies · Lipid binding proteins ·
Immunoprecipitation · Oxidative stress · Specificity · Organ & Tissue related research · Proteins · Value for money
General introduction
Hycult Biotech develops, produces and markets antibodies, antibody based products and more specifically immunoassays for innate immunity and directly related fields, with an emphasis on complement, neutrophil proteins, TLR, scavenger receptors and acute phase proteins. Furthermore, we have a strong interest in progressing research in the area of inflammation and cell damage caused by pathogens or oxidation factors.
Introduction product groupEndotoxin and other microbial toxins are involved in infectious diseases as well as in many other pathologies like atherosclerosis. The unraveling of the Toll-like receptor’s function in immunity has raised new interest in the innate immune response to bacterial toxins. Moreover, involvement of the acute phase response with LPS-binding proteins like LBP and Serum Amyloid A (SAA), has also contributed to this attention.
More information about products within the field of Innate Immunity, Antimicrobial peptides, Microbial toxins, TLR, Inflammation, Cell & Tissue damage, Lipid binding proteins and Oxidative stress can be found on our website. Please feel free to subscribe to our newsletter to remain up-to-date on Hycult Biotech’s product information!
Hycult Biotech has worldwide distribution through a network of over 45 distributors.
If you have any questions regarding pricing and ordering, please contact your local distributor. Distributor information can be found on our website.
We believe technical support is essential when using our products or when looking into buying them for your research needs. For product specific information please visit our website: www.hycultbiotech.com or contact us on [email protected].
In this Product Folder the following abbreviations for the applications have been used:FC: Flow CytometryFS: Functional studiesIA: ImmunoassaysIF: ImmunofluorescenceIHC: Immunohistochemistry F: Frozen sections P: Paraffin sectionsIP: ImmunoprecipitationW: Western Blot
macrophage
liver
tissue injury
TCC
PTX3
humoral innate immunity
bacteria
C1q
classicalcomplement
pathway
Factor H, Factor D, Factor Bbproperdin
alternativecomplement
pathway
IL-6
IL-1
SAA CRP haptoglobinfibrinogenceruloplasmin
HDL-3
C3, C4
SAP albumintransferrin
acute phaseresponse
complementactivation
increasedsynthesis
decreasedsynthesis
C3aC5a
Assays
Product description Quantity Cat.#
BPI, Human, ELISA kit 1 x 96 det. HK314-01
EndoCab IgA, Human, ELISA 1 x 96 det. HK504-IgA
EndoCab IgG, Human, ELISA 1 x 96 det. HK504-IgG
EndoCab IgM, Human, ELISA 1 x 96 det. HK504-IgM
EndoCab, Human, ELISA kit 1 x 96 det. HK504
LAL Chromogenic Endpoint Assay 3 x 96 det. HIT302
LBP, Human, ELISA kit 1 x 96 det. HK315-01
LBP, Mouse, ELISA kit 1 x 96 det. HK205-01
LBP, various species, ELISA kit 1 x 96 det. HK503
LL-37, Human, ELISA kit 1 x 96 det. HK321-01
Pentraxin 3, Human, ELISA kit 1 x 96 det. HK347-01
SAA, Human, ELISA kit 1 x 96 det. HK333-01
SAP, Mouse, ELISA 1 x 96 det. HK215-01
sCD14, Human, ELISA kit 1 x 96 det. HK320-01
SLPI, Human, ELISA kit 1 x 96 det. HK316-01
sTREM-1, Human, ELISA kit 1 x 96 det. HK348-01
TRACP5a, Human, ELISA kit 1 x 96 det. HK363-01
Product description Clone name Quantity Applications Cat. #
CD14, Human 18D11 100 µg FC FS HM2224CD14, Mouse Sa14-2 100 µg FC FS IF W HM1060CD14, Mouse, FITC Sa14-2 100 µg FC FS IF HM1060FCD14, Mouse, functional study antibody Sa14-2 0.5 mg FC FS IF HM1060-FSCD14, Mouse, R-PE labeled Sa14-2 25 tests FC FS IF HM1060PE-25CD14, Mouse, R-PE labeled Sa14-2 100 tests FC FS IF HM1060PE-100CNF1 NG8 100 µg FS IA W HM6005CNF1/CNF2 JC4 100 µg FS IA W HM6006EF-Tu, E.coli 900 100 µg IA IF W HM6010Lipid A 26-5 100 µg IA HM6009Lipid A, free 43 > 200 µg IA W HM2046Lipopolysaccharide Core WN1 222-5 100 µg FS IA P W HM6011Lipopolysaccharide Core, functional study antibody WN1 222-5 0.5 mg FS IA P W HM6011-FSLipopolysaccharide Core, immuno assay antibody WN1 222-5 0.5 mg FS IA P W HM6011-IALipoteichoic acid 55 > 200 µg F IA W HM2048LRG, Human 2F5.A2 100 µg IA HM2013Pentraxin 3, Mouse 2C3 100 µg FC FS IA IF IP W HM1130Pentraxin 3, Mouse 6B11 100 µg FC F FS IA W HM1131Polymyxin B 45 > 200 µg IA W HM2047Shigatoxin 1 13C4 100 µg FS IA W HM6007Shigatoxin 2 11E10 100 µg FS IA W HM6008sTREM-1, Human 15G7 100 µg IA HM2304TREM-1, Human 6B1 100 µg FC FS IA W HM2252TREM-1, Mouse L5-B8 100 µg FC FS IF W HM1098TREM-1, Mouse, FITC L5-B8 100 µg FC FS IF W HM1098FTREM-2, Mouse 6E9 100 µg FC IA IP HM1129TREM-2, Mouse, FITC 6E9 100 µg FC IA IP HM1129F
Antibodies
Figure 2: Serum Amyloid A deposits in glomerulus and arteriole of a kidney from a patient with amyloidosis. Staining with antibody Reu 86.1 (Cat.# HM2100).
Figure 3: Mouse CD14 in RAW cells. Staining with antibody Sa14-2 (Cat.# HM1060).
Figure 1: LPS staining within the colon lumen of SIV-infected rhesus macaques using antibody WN1 222-5 (Cat.# HM6011). Image courtesy of Dr. Jacob D. Estes from the Frederick National Laboratory for Cancer Research in Maryland, USA.
Hycult Biotech Frontstraat 2A5405 PB UdenThe Netherlands
T +31(0)413 251335F +31(0)413 248353E [email protected]
Hycult Biotech is distributed by:
Why scientists buy our products:· Robustness · Sensitivity · Specificity · Easy to use · Value for money · Two-plate kits · Functional studies · Technical support · · Validated assays · Quality control · Innate focus · Well documented products · Broad in applications ·
Hycult Biotech Inc.600 West Germantown PikeSuite 110Plymouth Meeting, PA 19462USAT: +1 484 5343566 Toll Free: 855-2-HYCULT (855-2-492858)F: +1 484 3683558E: [email protected]
Ligands
Proteins / Peptides
Product description Quantity Cat.#
Product description Quantity Cat.#
Lipid A from E.coli, Serotype R515 (Re) 1 mg HC4049
Lipid A from Salmonella minnesota, R595 (Re) 1 mg HC4057
LPS from E.coli Serotype O55:B5 1 mg HC4045
LPS from E.coli, Serotype EH100 (Ra) 1 mg HC4046
LPS from E.coli, Serotype J5 (Rc) 1 mg HC4047
LPS from E.coli, Serotype O8:K27 1 mg HC4065
LPS from E.coli, Serotype R515 (Re) 1 mg HC4048
LPS from Salmonella abortus equi, S-form 1 mg HC4061
LPS from Salmonella enteriditis, S-form 1 mg HC4059
LPS from Salmonella minnesota, R345 (Rb) 1 mg HC4053
LPS from Salmonella minnesota, R5 (Rc) 1 mg HC4054
LPS from Salmonella minnesota, R595 (Re) 1 mg HC4056
LPS from Salmonella minnesota, R7 (Rd) 1 mg HC4055
LPS from Salmonella minnesota, S-form 1 mg HC4051
LPS from Salmonella Minnesota. R60 (Ra) 1 mg HC4052
LPS from Salmonella typhimurium, S-form 1 mg HC4060
LPS set: 2 x LPS, 1 x Lipid A and 1 x MPL-A from E.coli 2x 0.5 mg and 2x 0.25 mg HC4064
LPS set: 2 x LPS, 1 x Lipid A and 1 x MPL-A from Salmonella minnesota 2x 0.5 mg and 2x 0.25 mg HC4063
MPL-A from E.coli, R515 (Re) 0.5 mg HC4050
MPL-A from Salmonella minnesota, R595 (Re) 0.5 mg HC4058
LALF, Peptide 1 mg HC4031-10
LALF, Peptide 0.5 mg HC4031-05
LALF, Peptide 100 µg HC4031-01
LBP, Human, Peptide 100 µg HC4030-01
LBP, Human, Peptide 0.5 mg HC4030-05
LBP, Human, Peptide 1 mg HC4030-10
LL-37/CAP-18, Human, Peptide 50 µg HC4013
Polymyxin B (PMB) 10E5 units HC4020
Polymyxin B (PMB), biotinylated 0.2 ml HC4021
Polymyxin B (PMB), HRP-conjugated 0.2 ml HC4022
Figure 4: Western blot detection of differentconcentrations of purified Proteinase 3 with mAbPR3G-2. The band size is approximately 30kDa(Cat.#HM2172).
Figure 5: Flow cytometric detection of granulocytesand monocytes with monoclonal antibody to MouseTREM-1 (mAb L5-B8, Cat.#HM1098).