Loose Connective Tissue
description
Transcript of Loose Connective Tissue
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Loose Connective Tissue
(LCT)
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Areolar Tissue
Pink = collagen
Purple = elastin
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Masson’s Trichrome Stain – blue = collagen
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Orecin Stain for Elastin – purple “ribbon candy”
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Sliver Stain for Reticular Fibers
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Collagen synthesis
and organization
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TEM of extracellular collagen fibers (Cl)
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TEM of collagen fibers with 64 nm periodicity
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Glycosaminoglycan Structure
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Proteoglycan
A schematic representation of a proteoglycan. It consists of a protein core molecule bound with many different types of Glycosaminoglycans (GAGs).
For example, a proteoglycan from cartilage matrix can have 30 keratan sulfate and 100 chondroitin sulfate GAG chains.
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Aggregan – the largest extracellular proteoglycan complex
Core hyaluronic acid (GAG in blue) complexed with numerous proteoglycans .
Aggregan – the largest extracellular proteoglycan complex
Core hyaluronic acid (GAG in blue) complexed with numerous proteoglycans .
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PAS stain of epithelial basement membranes (BM)
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TEM of a basement membrane (BL)
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Diagram of the Basal Lamina
Laminin
Nidogen
Heparan Sulfate
PGsCollagen Type IV
Basal cell memb w/ Integrins
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Cells of the LCT
Fibroblasts
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LM of fibroblasts (arrows)
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SEM of fibroblasts
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Live fibroblasts in culture – viewed with phase microscopy
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SEM image of a long fibroblast cell projection in culture
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Adipose and Adipocytes
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Adipocytes (fat cells)
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Adipose Tissue – fat cells + CT septa (arrows)
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Adipose Tissue fixed and stained w/ Osmium Tetroxide
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Defense cells of the LCT
Neutrophils, Plasma cells, Mast cells, and Macrophage
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LCT – a = pericytes; b = mast cell; c = plasma cells; d = capillary
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LCT – M = mast cell; P = plasma cells; F = fibroblasts; Eo = eosinophils, N = neutrophils
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TEM of a Plasma Cell
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TEM of a Mast Cell
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TEM of a Macrophage