LOCUS pSicoR-GFP 7567 bp DNA circular...

LOCUS pSicoR-GFP 7567 bp DNA circular 18-JUN-2004SOURCE ORGANISMCOMMENT This file is created by Vector NTI http://www.informaxinc.com/COMMENT VNTDATE|330781285|COMMENT VNTDBDATE|341686736|COMMENT VNTNAME|pSicoR-GFP|COMMENT VNTAUTHORNAME|Demo User|COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!)COMMENT (SXFCOMMENT (CGexDoc "pSicoR-GFP" 0 7567COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList)(CObList)COMMENT (CObList))COMMENT (CDocSetData 1 1 0 0 1 1 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 40 50 01 0COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)COMMENT (CStringList "AatI" "AatII" "Acc65I" "AccI" "AccIII" "AciI""AcsI"COMMENT "AcyI" "AflII" "AflIII" "AgeI" "AluI" "Alw44I" "AlwI" "AlwNI""AosI"COMMENT "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsnI" "Asp700" "Asp718""AspEI"COMMENT "AspHI" "AspI" "AsuII" "AvaI" "AvaII" "AviII" "AvrII" "BamHI""BanI"COMMENT "BanII" "BbrPI" "BbsI" "BbvI" "BcgI" "BclI" "BfaI" "BfrI" "BglI"COMMENT "BglII" "BlnI" "BmyI" "BpmI" "Bpu1102I" "BpuAI" "BsaAI" "BsaBI""BsaHI"COMMENT "BsaI" "BsaJI" "BsaWI" "BseAI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI""BsiYI"COMMENT "BslI" "BsmAI" "BsmFI" "BsmI" "Bsp120I" "Bsp1286I" "BspDI""BspEI"COMMENT "BspHI" "BspMI" "BspWI" "BsrBI" "BsrDI" "BsrFI" "BsrGI" "BsrI""BssHII"COMMENT "Bst1107I" "BstBI" "BstEII" "BstNI" "BstUI" "BstXI" "BstYI""Bsu36I"COMMENT "CelII" "CfoI" "Cfr10I" "ClaI" "Csp45I" "Csp6I" "DdeI" "DpnI""DpnII"COMMENT "DraI" "DraII" "DraIII" "DrdI" "DsaI" "DsaV" "EaeI" "EagI""Eam1105I"COMMENT "EarI" "Ecl136II" "EclXI" "Eco47III" "Eco57I" "EcoNI" "EcoO109I"COMMENT "EcoRI" "EcoRII" "EcoRV" "Esp3I" "EspI" "Fnu4HI" "FnuDII" "FokI""FspI"COMMENT "HaeII" "HaeIII" "HgaI" "HgiAI" "HhaI" "HincII" "HindII""HindIII"COMMENT "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "ItaI" "KasI" "KpnI""Ksp632I"

COMMENT "KspI" "MaeI" "MaeII" "MaeIII" "MamI" "MboI" "MboII" "McrI""MfeI"COMMENT "MluI" "MluNI" "MnlI" "MroI" "MscI" "MseI" "MslI" "MspA1I""MspI"COMMENT "MunI" "MvaI" "MvnI" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI""NdeII"COMMENT "NgoMI" "NheI" "NlaIII" "NlaIV" "NotI" "NruI" "NsiI" "NspI""NspV"COMMENT "PacI" "PaeR7I" "PflMI" "PinAI" "PleI" "PmaCI" "PmeI" "PmlI""Ppu10I"COMMENT "PpuMI" "Psp1406I" "PstI" "PvuI" "PvuII" "RcaI" "RsaI" "RsrII""SacI"COMMENT "SacII" "SalI" "SapI" "Sau3AI" "Sau96I" "ScaI" "ScrFI" "SexAI""SfaNI"COMMENT "SfcI" "SfiI" "SfuI" "SgrAI" "SmaI" "SnaBI" "SnoI" "SpeI" "SphI"COMMENT "SspBI" "SspI" "StuI" "StyI" "SwaI" "TaqI" "TfiI" "ThaI" "Tru9I"COMMENT "Tsp509I" "Tth111I" "Van91I" "XbaI" "XcmI" "XhoI" "XhoII" "XmaI"COMMENT "XmaIII" "XmnI") (CStringList "atg" "gtg")COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0COMMENT 4294967295 0 0 0 0 0 0) (CMolPar 0 0 0 1 7567 1 7567 0 0 0 0 0 00 0)COMMENT (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7)COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7)COMMENT (CObListCOMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 45 0 4666 0 60690 7315COMMENT 0)COMMENT #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 2320 0 2960 0 55480)COMMENT #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2971 0)COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 4452 0 5047 0)COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4349 0)COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 5 922 0 1478 02068 0COMMENT 4445 0 5651 0)COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 623 0 3376 0 36270)COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2430 0)COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0)COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0)COMMENT #10=(CRSite (CStringList) "AatI" "aggcct" 4 0 0)COMMENT #11=(CRSite (CStringList) "AatII" "gacgtc" 6 0 9 388 0 441 0 5240 710 0COMMENT 3141 0 3194 0 3277 0 3463 0 4910 0)COMMENT #12=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 5183 0)COMMENT #13=(CRSite (CStringList) "AccI" "gtmkac" 3 0 4 12 0 1353 0 25060 5056COMMENT 0) #14=(CRSite (CStringList) "AccIII" "tccgga" 2 0 1 5561 0)

COMMENT #15=(CRSite (CStringList) "AciI" "ccgc" 2 4 73 8 1 74 0 321 0 3490 361COMMENT 0 375 0 542 0 633 1 666 1 770 0 791 1 1125 1 1160 1 1206 11523 0COMMENT 1534 1 1542 0 2622 0 2641 0 2656 0 2951 1 2982 1 2986 0 30060 3074COMMENT 0 3102 0 3114 0 3128 0 3295 0 3386 1 3419 1 3523 0 3544 13605 0COMMENT 3847 0 3888 0 3955 0 3994 0 4132 0 4245 0 4305 0 4308 0 47661 4779COMMENT 0 4792 0 4902 1 4943 1 4957 0 4959 1 4978 1 4988 0 5034 05042 0COMMENT 5450 0 5525 0 5800 0 5828 0 5955 0 5974 0 6095 0 6205 1 63400 6349COMMENT 1 6711 0 6802 0 6993 1 7039 0 7160 1 7204 0 7281 0 7390 07489 1COMMENT 7536 0)COMMENT #16=(CRSite (CStringList) "AcsI" "raatty" 2 0 7 140 0 1148 0 12350 2560COMMENT 0 2567 0 4349 0 4476 0)COMMENT #17=(CRSite (CStringList) "AcyI" "grcgyc" 3 0 12 385 0 438 0 5210 707 0COMMENT 1029 0 2638 0 3138 0 3191 0 3274 0 3460 0 4907 0 7185 0)COMMENT #18=(CRSite (CStringList) "AflII" "cttaag" 2 0 3 3 0 908 0 56370)COMMENT #19=(CRSite (CStringList) "AflIII" "acrygt" 2 0 3 241 0 2861 04402 0)COMMENT #20=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 3616 0)COMMENT #21=(CRSite (CStringList) "AluI" "agct" 3 0 37 148 0 880 0 924 01071 0COMMENT 1302 0 1371 0 1480 0 1700 0 1876 0 1924 0 2070 0 2691 0 27960 3582COMMENT 0 3649 0 3682 0 3754 0 3787 0 4003 0 4051 0 4162 0 4336 04447 0COMMENT 4723 0 5112 0 5214 0 5396 0 5555 0 5609 0 5653 0 5923 0 60130 6059COMMENT 0 6316 0 6837 0 6937 0 7000 0)COMMENT #22=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 4 45 0 4666 06069 0COMMENT 7315 0)COMMENT #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 26 25 0 28 1 978 11401 0COMMENT 1923 0 2376 1 2410 0 2592 1 2626 1 2966 1 2979 0 3596 1 41231 4322COMMENT 0 5027 0 5310 0 5367 0 5707 1 6316 1 6402 1 6404 0 6500 16501 0COMMENT 6964 1 7281 0 7285 1)

COMMENT #24=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 4 93 0 1843 01927 0COMMENT 6171 0) #25=(CRSite (CStringList) "AosI" "tgcgca" 4 0 1 68700)COMMENT #26=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2622 0)COMMENT #27=(CRSite (CStringList) "ApoI" "raatty" 2 0 7 140 0 1148 0 12350 2560COMMENT 0 2567 0 4349 0 4476 0)COMMENT #28=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0)COMMENT #29=(CRSite (CStringList) "AseI" "attaat" 3 0 3 270 0 3023 0 68200)COMMENT #30=(CRSite (CStringList) "AsnI" "attaat" 3 0 3 270 0 3023 0 68200)COMMENT #31=(CRSite (CStringList) "Asp700" "gaannnnttc" 6 0 2 1235 0 72470)COMMENT #32=(CRSite (CStringList) "Asp718" "ggtacc" 2 0 1 5183 0)COMMENT #33=(CRSite (CStringList) "AspEI" "gacnnnnngtc" 7 0 1 6648 0)COMMENT #34=(CRSite (CStringList) "AspHI" "gwgcwc" 6 0 9 49 0 882 0 10730 4238COMMENT 0 4670 0 5611 0 6073 0 7234 0 7319 0)COMMENT #35=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 0)COMMENT #36=(CRSite (CStringList) "AsuII" "ttcgaa" 3 0 0)COMMENT #37=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 6 2327 0 4285 04361 0COMMENT 4945 0 6786 0 7008 0)COMMENT #38=(CRSite (CStringList) "AviII" "tgcgca" 4 0 1 6870 0)COMMENT #39=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0)COMMENT #40=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 6 728 0 1028 0 34810 3664COMMENT 0 5183 0 6596 0)COMMENT #41=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 882 0 1073 02622 0COMMENT 4810 0 5611 0) #42=(CRSite (CStringList) "BbrPI" "cacgtg" 40 0)COMMENT #43=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 4985 1 5285 0)COMMENT #44=(CRSite (CStringList) "BbvI" "gcagc" 14 18 27 200 1 836 01736 0COMMENT 1757 0 1810 0 1813 0 1885 0 3774 1 3880 0 4164 0 4171 0 41971 4200COMMENT 1 4525 1 4781 1 4857 1 4953 1 5121 0 5223 0 5542 1 5764 06084 1COMMENT 6174 0 6177 0 6383 0 6686 1 7077 0)COMMENT #45=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 2 3753 07187 0)COMMENT #46=(CRSite (CStringList) "BclI" "tgatca" 2 0 0)COMMENT #47=(CRSite (CStringList) "BfaI" "ctag" 2 0 25 263 0 893 0 979 01020 0

COMMENT 1157 0 1167 0 1303 0 1986 0 2627 0 2650 0 2735 0 2793 0 29680 3608COMMENT 0 4426 0 5073 0 5136 0 5397 0 5529 0 5622 0 5708 0 5749 06250 0COMMENT 6503 0 6838 0)COMMENT #48=(CRSite (CStringList) "BfrI" "cttaag" 2 0 3 3 0 908 0 5637 0)COMMENT #49=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 7 353 0 475 0546 0COMMENT 3106 0 3228 0 3299 0 6768 0)COMMENT #50=(CRSite (CStringList) "BglII" "agatct" 2 0 4 25 0 864 0 52190 5593COMMENT 0) #51=(CRSite (CStringList) "BlnI" "cctagg" 2 0 0)COMMENT #52=(CRSite (CStringList) "BmyI" "gdgchc" 6 0 17 49 0 882 0 955 01073 0COMMENT 2622 0 3669 0 3798 0 4047 0 4238 0 4670 0 4810 0 4823 0 56110 5684COMMENT 0 6073 0 7234 0 7319 0)COMMENT #53=(CRSite (CStringList) "BpmI" "ctggag" 23 21 5 1581 0 1862 14073 0COMMENT 4313 0 6718 1)COMMENT #54=(CRSite (CStringList) "Bpu1102I" "gctnagc" 3 0 0)COMMENT #55=(CRSite (CStringList) "BpuAI" "gaagac" 9 13 2 4985 1 5285 0)COMMENT #56=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 2 603 0 3356 0)COMMENT #57=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 0)COMMENT #58=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 12 385 0 438 0 5210 707COMMENT 0 1029 0 2638 0 3138 0 3191 0 3274 0 3460 0 4907 0 7185 0)COMMENT #59=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 4 852 0 5062 15581 0COMMENT 6709 1)COMMENT #60=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 26 623 0 873 01249 0COMMENT 1472 0 1647 0 1708 0 1927 0 1976 0 2321 0 2708 0 2926 0 33760 3627COMMENT 0 3657 0 3797 0 3960 0 3984 0 4039 0 4761 0 4833 0 4865 04957 0COMMENT 5349 0 5355 0 5602 0 5915 0)COMMENT #61=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 6 3616 0 4416 05561 0COMMENT 5961 0 6108 0 6939 0)COMMENT #62=(CRSite (CStringList) "BseAI" "tccgga" 2 0 1 5561 0)COMMENT #63=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 6 1688 0 1817 02509 0COMMENT 3757 1 3854 0 4178 0)COMMENT #64=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 9 12 0 1538 0 15420 2986COMMENT 0 3621 0 5066 0 6095 0 7018 0 7167 0)

COMMENT #65=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 9 49 0 882 01073 0COMMENT 4238 0 4670 0 5611 0 6073 0 7234 0 7319 0)COMMENT #66=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0)COMMENT #67=(CRSite (CStringList) "BsiYI" "ccnnnnnnngg" 8 0 24 312 0 8430 1038COMMENT 0 1565 0 1897 0 2650 0 2709 0 2981 0 3065 0 3798 0 3961 04311 0COMMENT 4647 0 4698 0 4797 0 4866 0 4958 0 4969 0 5355 0 5383 0 57770 5795COMMENT 0 5961 0 6240 0)COMMENT #68=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 24 312 0 843 01038 0COMMENT 1565 0 1897 0 2650 0 2709 0 2981 0 3065 0 3798 0 3961 0 43110 4647COMMENT 0 4698 0 4797 0 4866 0 4958 0 4969 0 5355 0 5383 0 5777 05795 0COMMENT 5961 0 6240 0)COMMENT #69=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 11 697 0 852 02364 1COMMENT 2370 1 2905 1 3450 0 4623 0 5062 1 5581 0 6709 1 7485 0)COMMENT #70=(CRSite (CStringList) "BsmFI" "gggac" 16 20 17 438 0 589 0757 0COMMENT 1054 0 1379 0 1592 0 2057 0 2340 0 2601 0 3191 0 3342 0 35100 4374COMMENT 0 4747 0 4907 1 4918 0 5263 0)COMMENT #71=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 1 1987 0)COMMENT #72=(CRSite (CStringList) "Bsp120I" "gggccc" 2 0 1 2618 0)COMMENT #73=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 17 49 0 882 0955 0COMMENT 1073 0 2622 0 3669 0 3798 0 4047 0 4238 0 4670 0 4810 0 48230 5611COMMENT 0 5684 0 6073 0 7234 0 7319 0)COMMENT #74=(CRSite (CStringList) "BspDI" "atcgat" 3 0 2 4452 0 5047 0)COMMENT #75=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 5561 0)COMMENT #76=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 6475 0 7483 0)COMMENT #77=(CRSite (CStringList) "BspMI" "acctgc" 11 15 0)COMMENT #78=(CRSite (CStringList) "BspWI" "gcnnnnnnngc" 8 0 42 210 0 3530 475 0COMMENT 507 0 546 0 639 0 663 0 833 0 934 0 1108 0 1531 0 1543 01740 0COMMENT 1829 0 1846 0 2104 0 2414 0 2425 0 2756 0 3106 0 3228 0 32600 3299COMMENT 0 3392 0 3416 0 3720 0 3780 0 3793 0 3837 0 3846 0 4665 04787 0COMMENT 4791 0 4883 0 4975 0 4987 0 5118 0 5127 0 5663 0 5808 0 63800 6768

COMMENT 0) #79=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 2 2624 17489 0)COMMENT #80=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 4704 1 6709 06883 1)COMMENT #81=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 6 1537 0 2660 03616 0COMMENT 3779 0 4969 0 6728 0)COMMENT #82=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 4338 0)COMMENT #83=(CRSite (CStringList) "BsrI" "actgg" 7 5 22 87 1 558 1 847 01136 0COMMENT 1367 0 2601 1 3311 1 4240 1 4498 0 4699 0 5169 1 5257 0 54031 5576COMMENT 0 6163 0 6176 0 6288 1 6694 1 6812 1 6855 1 7124 0 7294 1)COMMENT #84=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 1 1102 0)COMMENT #85=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 0)COMMENT #86=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0)COMMENT #87=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 2953 0)COMMENT #88=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 26 353 0 546 0 8740 1250COMMENT 0 1325 0 1560 0 1880 0 1892 0 1928 0 2032 0 3106 0 3299 03674 0COMMENT 3799 0 3911 0 3986 0 4040 0 4609 0 4891 0 5132 0 5350 0 53560 5603COMMENT 0 5783 0 5904 0 5917 0)COMMENT #89=(CRSite (CStringList) "BstUI" "cgcg" 3 0 23 133 0 221 0 243 0323 0COMMENT 829 0 1104 0 1224 0 1544 0 2658 0 2949 0 2951 0 2988 0 30760 3957COMMENT 0 4275 0 4902 0 4959 0 4990 0 5802 0 6383 0 6713 0 7206 07538 0)COMMENT #90=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0)COMMENT #91=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 20 25 0 864 0 9830 2381COMMENT 0 2597 0 2631 0 2971 0 3601 0 4128 0 5019 0 5219 0 5302 05593 0COMMENT 5712 0 6396 0 6407 0 6493 0 6505 0 7273 0 7290 0)COMMENT #92=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 1 5179 0)COMMENT #93=(CRSite (CStringList) "CelII" "gctnagc" 3 0 0)COMMENT #94=(CRSite (CStringList) "CfoI" "gcgc" 4 0 26 133 0 213 0 829 01031 0COMMENT 1104 0 1106 0 1546 0 1745 0 1834 0 2419 0 2660 0 2951 0 36130 3918COMMENT 0 3959 0 4275 0 4902 0 5935 0 6002 0 6102 0 6276 0 6385 06778 0COMMENT 6871 0 7208 0 7540 0)COMMENT #95=(CRSite (CStringList) "Cfr10I" "rccggy" 2 0 6 1537 0 2660 03616 0COMMENT 3779 0 4969 0 6728 0)

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COMMENT 2052 0 2059 0 2085 0 2126 0 2135 0 2161 0 2179 0 2198 02421 0COMMENT 2453 0 2538 0 2551 0 2560 0 2567 0 2715 0 2897 0 3034 04349 0COMMENT 4433 0 4476 0 4828 0 5263 0 6515 0 6821 0 7076 0)COMMENT #221=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0)COMMENT #222=(CRSite (CStringList) "Van91I" "ccannnnntgg" 8 0 1 4698 0)COMMENT #223=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2626 0)COMMENT #224=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 0)COMMENT #225=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2960 0)COMMENT #226=(CRSite (CStringList) "XhoII" "rgatcy" 2 0 20 25 0 864 0 9830 2381COMMENT 0 2597 0 2631 0 2971 0 3601 0 4128 0 5019 0 5219 0 5302 05593 0COMMENT 5712 0 6396 0 6407 0 6493 0 6505 0 7273 0 7290 0)COMMENT #227=(CRSite (CStringList) "XmaIII" "cggccg" 2 0 3 1535 0 1539 02983 0)COMMENT #228=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 1235 0 72470))COMMENT (CObListCOMMENT #229=(CFSignal (CObList) "CMV-GFP" 51 0 0 2983 4353 0(CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #230=(CFSignal (CObList) "Psi" 21 0 0 1076 1213 0 (CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #231=(CFSignal (CObList) "RRE" 21 0 0 1707 1910 0 (CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #232=(CFSignal (CObList) "shRNA cloning site" 21 0 0 2945 2960 0COMMENT (CStringList) (CStringList) 1 1 1 1 "")COMMENT #233=(CFSignal (CObList) "U6 Promotor" 29 0 0 2631 2944 0(CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #234=(CFSignal (CObList) "5' LoxP" 21 0 0 2826 2859 0(CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #235=(CFSignal (CObList) "3' LoxP" 21 0 0 4367 4400 0(CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #236=(CFSignal (CObList) "WRE" 21 0 0 4456 4835 0 (CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #237=(CFSignal (CObList) "Lox replace for" 27 0 0 2481 2500 0COMMENT (CStringList) (CStringList) 1 1 1 1 "")COMMENT #238=(CFSignal (CObList) "Loop out for" 27 0 0 2708 2733 0(CStringList)COMMENT (CStringList) 1 1 1 1 "")COMMENT #239=(CFSignal (CObList) "Loop out rev" 27 0 0 4538 4561 0(CStringList)COMMENT (CStringList) 1 1 1 1 "")

COMMENT #240=(CFSignal (CObList) "Lox replace rev" 27 0 0 493 512 0COMMENT (CStringList) (CStringList) 1 1 1 1 "")COMMENT #241=(CFSignal (CObList) "Lox replace rev" 27 0 0 3246 3265 0COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList)(CObList)COMMENT (CObList) (CObList) (CObList) (CObList)COMMENT (CTextView 0COMMENT #242=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180)COMMENT (CObjectListCOMMENT #243=(CRefLineParCOMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSicoR-GFP" 2)COMMENT 5 "" 0 4)COMMENT #244=(CFolderParCOMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178)COMMENT (CObjectListCOMMENT #245=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "DNA 'pSicoR-GFP'" 1)COMMENT #246=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "Local object" 1)COMMENT #247=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "Created: 02/20/04 11:41 AM" 1)COMMENT #248=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "Last Modified: 06/18/04 04:58 PM" 1)COMMENT #249=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "length: 7567 bp" 1)COMMENT #250=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "storage type: Basic" 1)COMMENT #251=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)COMMENT "form: Circular" 1))) "General Description")COMMENT #252=(CFolderParCOMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178)(CObjectList))COMMENT "Standard Fields")COMMENT #253=(CRefLineParCOMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233)"Comments" 2) 1COMMENT "" 0 0)COMMENT #254=(CFolderParCOMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178)(CObjectList))COMMENT "Annotations")COMMENT #255=(CFolderParCOMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178)COMMENT (CObjectListCOMMENT #256=(CFolderParCOMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0178)

COMMENT (CObjectListCOMMENT #257=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 1076 (3 #230# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #258=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 1076 End: 1213"1))) "Psi")COMMENT #259=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 1707 (3 #231# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #260=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 1707 End: 1910"1))) "RRE")COMMENT #261=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 2826 (3 #234# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #262=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 2826 End: 2859"1)))COMMENT "5' LoxP")COMMENT #263=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 2945 (3 #232# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #264=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 2945 End: 2960"1)))COMMENT "shRNA cloning site")COMMENT #265=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 4367 (3 #235# 0) 1 2 20 194)COMMENT (CObjectList

COMMENT #266=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 4367 End: 4400"1)))COMMENT "3' LoxP")COMMENT #267=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 4456 (3 #236# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #268=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 4456 End: 4835"1))) "WRE")))COMMENT "Misc. Feature (6 total)")COMMENT #269=(CFolderParCOMMENT (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0178)COMMENT (CObjectListCOMMENT #270=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 493 (3 #240# 0) 1 2 2 0194)COMMENT (CObjectListCOMMENT #271=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 493 End: 512 "1)))COMMENT "Lox replace rev")COMMENT #272=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 2481 (3 #237# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #273=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 2481 End: 2500"1)))COMMENT "Lox replace for")COMMENT #274=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 2708 (3 #238# 0) 1 2 20 194)COMMENT (CObjectList

COMMENT #275=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 2708 End: 2733"1)))COMMENT "Loop out for")COMMENT #276=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 3246 (3 #241# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #277=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 3246 End: 3265"1)))COMMENT "Lox replace rev")COMMENT #278=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 4538 (3 #239# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #279=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 4538 End: 4561"1)))COMMENT "Loop out rev"))) "Primer (5total)")COMMENT #280=(CFolderParCOMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0178)COMMENT (CObjectListCOMMENT #281=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 2631 (3 #233# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #282=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 2631 End: 2944"1)))COMMENT "U6 Promotor")))COMMENT "Promoter Eukaryotic (1 total)")COMMENT #283=(CFolderParCOMMENT (CGroupPar (CParagraph 51 (7 51 0) 1 1 1 0178)

COMMENT (CObjectListCOMMENT #284=(CFolderParCOMMENT (CGroupParCOMMENT (CParagraph 2983 (3 #229# 0) 1 2 20 194)COMMENT (CObjectListCOMMENT #285=(CLineParCOMMENT (CParagraph 0 (0 0) 1 2 3 0180)COMMENT "Start: 2983 End: 4353"1)))COMMENT "CMV-GFP"))) "5' Clip (1 total)")))COMMENT "Feature Map")COMMENT #286=(CFolderParCOMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178)COMMENT (CObjectListCOMMENT #287=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SmaI: 0 sites") 3 3"CCCGGG"COMMENT "GGGCCC")COMMENT #288=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "XmaI: 0 sites") 1 5"CCCGGG"COMMENT "GGGCCC")COMMENT #289=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AatI: 0 sites") 3 3"AGGCCT"COMMENT "TCCGGA")COMMENT #290=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AscI: 0 sites") 2 6"GGCGCGCC"COMMENT "CCGCGCGG")COMMENT #291=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AspI: 0 sites") 4 5"GACNNNGTC"COMMENT "CTGNNNCAG")COMMENT #292=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AsuII: 0 sites") 2 4"TTCGAA"COMMENT "AAGCTT")COMMENT #293=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AvrII: 0 sites") 1 5"CCTAGG"COMMENT "GGATCC")COMMENT #294=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BbrPI: 0 sites") 3 3"CACGTG"COMMENT "GTGCAC")COMMENT #295=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BclI: 0 sites") 1 5"TGATCA"COMMENT "ACTAGT")COMMENT #296=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BlnI: 0 sites") 1 5"CCTAGG"COMMENT "GGATCC")COMMENT #297=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Bpu1102I: 0 sites") 2 5"GCTNAGC"COMMENT "CGANTCG")COMMENT #298=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsaBI: 0 sites") 5 5"GATNNNNATC"COMMENT "CTANNNNTAG")COMMENT #299=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsiWI: 0 sites") 1 5"CGTACG"COMMENT "GCATGC")COMMENT #300=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BspMI: 0 sites") 10 14COMMENT "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN")COMMENT #301=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Bst1107I: 0 sites") 3 3"GTATAC"COMMENT "CATATG")COMMENT #302=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BstBI: 0 sites") 2 4"TTCGAA"COMMENT "AAGCTT")COMMENT #303=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BstXI: 0 sites") 8 4COMMENT "CCANNNNNNTGG" "GGTNNNNNNACC")COMMENT #304=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "CelII: 0 sites") 2 5"GCTNAGC"COMMENT "CGANTCG")COMMENT #305=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Csp45I: 0 sites") 2 4"TTCGAA"COMMENT "AAGCTT")COMMENT #306=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Esp3I: 0 sites") 7 11COMMENT "CGTCTCNNNNNN" "GCAGAGNNNNNN")COMMENT #307=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EspI: 0 sites") 2 5"GCTNAGC"COMMENT "CGANTCG")COMMENT #308=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "MamI: 0 sites") 5 5"GATNNNNATC"

COMMENT "CTANNNNTAG")COMMENT #309=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "MluNI: 0 sites") 3 3"TGGCCA"COMMENT "ACCGGT")COMMENT #310=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "MscI: 0 sites") 3 3"TGGCCA"COMMENT "ACCGGT")COMMENT #311=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NspV: 0 sites") 2 4"TTCGAA"COMMENT "AAGCTT")COMMENT #312=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PacI: 0 sites") 5 3"TTAATTAA"COMMENT "AATTAATT")COMMENT #313=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PmaCI: 0 sites") 3 3"CACGTG"COMMENT "GTGCAC")COMMENT #314=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PmeI: 0 sites") 4 4"GTTTAAAC"COMMENT "CAAATTTG")COMMENT #315=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PmlI: 0 sites") 3 3"CACGTG"COMMENT "GTGCAC")COMMENT #316=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "RsrII: 0 sites") 2 5"CGGWCCG"

COMMENT "GCCWGGC")COMMENT #317=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SexAI: 0 sites") 1 6"ACCWGGT"COMMENT "TGGWCCA")COMMENT #318=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SfiI: 0 sites") 8 5COMMENT "GGCCNNNNNGGCC" "CCGGNNNNNCCGG")COMMENT #319=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SfuI: 0 sites") 2 4"TTCGAA"COMMENT "AAGCTT")COMMENT #320=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SgrAI: 0 sites") 2 6"CRCCGGYG"COMMENT "GYGGCCRC")COMMENT #321=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SphI: 0 sites") 5 1"GCATGC"COMMENT "CGTACG")COMMENT #322=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "StuI: 0 sites") 3 3"AGGCCT"COMMENT "TCCGGA")COMMENT #323=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SwaI: 0 sites") 4 4"ATTTAAAT"COMMENT "TAAATTTA")COMMENT #324=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Tth111I: 0 sites") 4 5COMMENT "GACNNNGTC" "CTGNNNCAG")COMMENT #325=(CRSFolderPar

COMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "XcmI: 0 sites") 8 7COMMENT "CCANNNNNNNNNTGG" "GGTNNNNNNNNNACC")COMMENT #326=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #327=(CGroupParCOMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #328=(CLineParCOMMENT (CParagraph 0 (1 #2# 1) 1 22 0 191)COMMENT " N1: 2971 " 1))))) "BamHI:1 site")COMMENT 1 5 "GGATCC" "CCTAGG")COMMENT #329=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #330=(CGroupParCOMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #331=(CLineParCOMMENT (CParagraph 0 (1 #4# 1) 1 22 0 191)COMMENT " N1: 4349 " 1))))) "EcoRI:1 site")COMMENT 1 5 "GAATTC" "CTTAAG")COMMENT #332=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #333=(CGroupParCOMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #334=(CLineParCOMMENT (CParagraph 0 (1 #7# 1) 1 22 0 191)COMMENT " N1: 2430 " 1))))) "PstI: 1site")COMMENT 5 1 "CTGCAG" "GACGTC")COMMENT #335=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #336=(CGroupParCOMMENT (CParagraph 0 (10 #12# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #337=(CLineParCOMMENT (CParagraph 0 (1 #12# 1) 1 22 0COMMENT 191) " N1: 5183 " 1)))))COMMENT "Acc65I: 1 site") 1 5 "GGTACC" "CCATGG")COMMENT #338=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #339=(CGroupParCOMMENT (CParagraph 0 (10 #14# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #340=(CLineParCOMMENT (CParagraph 0 (1 #14# 1) 1 22 0COMMENT 191) " N1: 5561 " 1)))))COMMENT "AccIII: 1 site") 1 5 "TCCGGA" "AGGCCT")COMMENT #341=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #342=(CGroupParCOMMENT (CParagraph 0 (10 #20# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #343=(CLineParCOMMENT (CParagraph 0 (1 #20# 1) 1 22 0COMMENT 191) " N1: 3616 " 1)))))COMMENT "AgeI: 1 site") 1 5 "ACCGGT" "TGGCCA")COMMENT #344=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #345=(CGroupParCOMMENT (CParagraph 0 (10 #25# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #346=(CLineParCOMMENT (CParagraph 0 (1 #25# 1) 1 22 0

COMMENT 191) " N1: 6870 " 1)))))COMMENT "AosI: 1 site") 3 3 "TGCGCA" "ACGCGT")COMMENT #347=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #348=(CGroupParCOMMENT (CParagraph 0 (10 #26# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #349=(CLineParCOMMENT (CParagraph 0 (1 #26# 1) 1 22 0COMMENT 191) " N1: 2622 " 1)))))COMMENT "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG")COMMENT #350=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #351=(CGroupParCOMMENT (CParagraph 0 (10 #32# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #352=(CLineParCOMMENT (CParagraph 0 (1 #32# 1) 1 22 0COMMENT 191) " N1: 5183 " 1)))))COMMENT "Asp718: 1 site") 1 5 "GGTACC" "CCATGG")COMMENT #353=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #354=(CGroupParCOMMENT (CParagraph 0 (10 #33# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #355=(CLineParCOMMENT (CParagraph 0 (1 #33# 1) 1 22 0COMMENT 191) " N1: 6648 " 1)))))COMMENT "AspEI: 1 site") 6 5 "GACNNNNNGTC""CTGNNNNNCAG")COMMENT #356=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #357=(CGroupPar

COMMENT (CParagraph 0 (10 #38# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #358=(CLineParCOMMENT (CParagraph 0 (1 #38# 1) 1 22 0COMMENT 191) " N1: 6870 " 1)))))COMMENT "AviII: 1 site") 3 3 "TGCGCA" "ACGCGT")COMMENT #359=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #360=(CGroupParCOMMENT (CParagraph 0 (10 #62# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #361=(CLineParCOMMENT (CParagraph 0 (1 #62# 1) 1 22 0COMMENT 191) " N1: 5561 " 1)))))COMMENT "BseAI: 1 site") 1 5 "TCCGGA" "AGGCCT")COMMENT #362=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #363=(CGroupParCOMMENT (CParagraph 0 (10 #71# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #364=(CLineParCOMMENT (CParagraph 0 (1 #71# 1) 1 22 0COMMENT 191) " N1: 1987 " 1)))))COMMENT "BsmI: 1 site") 7 5 "GAATGCNN" "CTTACGNN")COMMENT #365=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #366=(CGroupParCOMMENT (CParagraph 0 (10 #72# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #367=(CLineParCOMMENT (CParagraph 0 (1 #72# 1) 1 22 0COMMENT 191) " N1: 2618 " 1)))))COMMENT "Bsp120I: 1 site") 1 5 "GGGCCC" "CCCGGG")COMMENT #368=(CRSFolderPar

COMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #369=(CGroupParCOMMENT (CParagraph 0 (10 #75# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #370=(CLineParCOMMENT (CParagraph 0 (1 #75# 1) 1 22 0COMMENT 191) " N1: 5561 " 1)))))COMMENT "BspEI: 1 site") 1 5 "TCCGGA" "AGGCCT")COMMENT #371=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #372=(CGroupParCOMMENT (CParagraph 0 (10 #82# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #373=(CLineParCOMMENT (CParagraph 0 (1 #82# 1) 1 22 0COMMENT 191) " N1: 4338 " 1)))))COMMENT "BsrGI: 1 site") 1 5 "TGTACA" "ACATGT")COMMENT #374=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #375=(CGroupParCOMMENT (CParagraph 0 (10 #84# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #376=(CLineParCOMMENT (CParagraph 0 (1 #84# 1) 1 22 0COMMENT 191) " N1: 1102 " 1)))))COMMENT "BssHII: 1 site") 1 5 "GCGCGC" "CGCGCG")COMMENT #377=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #378=(CGroupParCOMMENT (CParagraph 0 (10 #87# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #379=(CLinePar

COMMENT (CParagraph 0 (1 #87# 1) 1 22 0COMMENT 191) " N1: 2953 " 1)))))COMMENT "BstEII: 1 site") 1 6 "GGTNACC" "CCANTGG")COMMENT #380=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #381=(CGroupParCOMMENT (CParagraph 0 (10 #92# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #382=(CLineParCOMMENT (CParagraph 0 (1 #92# 1) 1 22 0COMMENT 191) " N1: 5179 " 1)))))COMMENT "Bsu36I: 1 site") 2 5 "CCTNAGG" "GGANTCC")COMMENT #383=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #384=(CGroupParCOMMENT (CParagraph 0 (10 #104# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #385=(CLineParCOMMENT (CParagraph 0 (1 #104# 1) 12 2 0COMMENT 191) " N1: 5863 " 1)))))COMMENT "DrdI: 1 site") 7 5 "GACNNNNNNGTC""CTGNNNNNNCAG")COMMENT #386=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #387=(CGroupParCOMMENT (CParagraph 0 (10 #109# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #388=(CLineParCOMMENT (CParagraph 0 (1 #109# 1) 12 2 0COMMENT 191) " N1: 6648 " 1)))))COMMENT "Eam1105I: 1 site") 6 5 "GACNNNNNGTC"COMMENT "CTGNNNNNCAG")COMMENT #389=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)

COMMENT (CObjectListCOMMENT #390=(CGroupParCOMMENT (CParagraph 0 (10 #113# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #391=(CLineParCOMMENT (CParagraph 0 (1 #113# 1) 12 2 0COMMENT 191) " N1: 3612 " 1)))))COMMENT "Eco47III: 1 site") 3 3 "AGCGCT" "TCGCGA")COMMENT #392=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #393=(CGroupParCOMMENT (CParagraph 0 (10 #115# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #394=(CLineParCOMMENT (CParagraph 0 (1 #115# 1) 12 2 0COMMENT 191) " N1: 1563 " 1)))))COMMENT "EcoNI: 1 site") 5 6 "CCTNNNNNAGG""GGANNNNNTCC")COMMENT #395=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #396=(CGroupParCOMMENT (CParagraph 0 (10 #124# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #397=(CLineParCOMMENT (CParagraph 0 (1 #124# 1) 12 2 0COMMENT 191) " N1: 6870 " 1)))))COMMENT "FspI: 1 site") 3 3 "TGCGCA" "ACGCGT")COMMENT #398=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #399=(CGroupParCOMMENT (CParagraph 0 (10 #134# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #400=(CLineParCOMMENT (CParagraph 0 (1 #134# 1) 12 2 0

COMMENT 191) " N1: 2945 " 1)))))COMMENT "HpaI: 1 site") 3 3 "GTTAAC" "CAATTG")COMMENT #401=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #402=(CGroupParCOMMENT (CParagraph 0 (10 #138# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #403=(CLineParCOMMENT (CParagraph 0 (1 #138# 1) 12 2 0COMMENT 191) " N1: 1028 " 1)))))COMMENT "KasI: 1 site") 1 5 "GGCGCC" "CCGCGG")COMMENT #404=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #405=(CGroupParCOMMENT (CParagraph 0 (10 #139# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #406=(CLineParCOMMENT (CParagraph 0 (1 #139# 1) 12 2 0COMMENT 191) " N1: 5187 " 1)))))COMMENT "KpnI: 1 site") 5 1 "GGTACC" "CCATGG")COMMENT #407=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #408=(CGroupParCOMMENT (CParagraph 0 (10 #141# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #409=(CLineParCOMMENT (CParagraph 0 (1 #141# 1) 12 2 0COMMENT 191) " N1: 4960 " 1)))))COMMENT "KspI: 1 site") 4 2 "CCGCGG" "GGCGCC")COMMENT #410=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #411=(CGroupParCOMMENT (CParagraph 0 (10 #150# 0) 1 2 2 0180)

COMMENT (CObjectListCOMMENT #412=(CLineParCOMMENT (CParagraph 0 (1 #150# 1) 12 2 0COMMENT 191) " N1: 241 " 1)))))COMMENT "MluI: 1 site") 1 5 "ACGCGT" "TGCGCA")COMMENT #413=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #414=(CGroupParCOMMENT (CParagraph 0 (10 #153# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #415=(CLineParCOMMENT (CParagraph 0 (1 #153# 1) 12 2 0COMMENT 191) " N1: 5561 " 1)))))COMMENT "MroI: 1 site") 1 5 "TCCGGA" "AGGCCT")COMMENT #416=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #417=(CGroupParCOMMENT (CParagraph 0 (10 #164# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #418=(CLineParCOMMENT (CParagraph 0 (1 #164# 1) 12 2 0COMMENT 191) " N1: 1029 " 1)))))COMMENT "NarI: 1 site") 2 4 "GGCGCC" "CCGCGG")COMMENT #419=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #420=(CGroupParCOMMENT (CParagraph 0 (10 #169# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #421=(CLineParCOMMENT (CParagraph 0 (1 #169# 1) 12 2 0COMMENT 191) " N1: 3607 " 1)))))COMMENT "NheI: 1 site") 1 5 "GCTAGC" "CGATCG")COMMENT #422=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)

COMMENT (CObjectListCOMMENT #423=(CGroupParCOMMENT (CParagraph 0 (10 #172# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #424=(CLineParCOMMENT (CParagraph 0 (1 #172# 1) 12 2 0COMMENT 191) " N1: 2983 " 1)))))COMMENT "NotI: 1 site") 2 6 "GCGGCCGC" "CGCCGGCG")COMMENT #425=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #426=(CGroupParCOMMENT (CParagraph 0 (10 #174# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #427=(CLineParCOMMENT (CParagraph 0 (1 #174# 1) 12 2 0COMMENT 191) " N1: 3015 " 1)))))COMMENT "NsiI: 1 site") 5 1 "ATGCAT" "TACGTA")COMMENT #428=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #429=(CGroupParCOMMENT (CParagraph 0 (10 #178# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #430=(CLineParCOMMENT (CParagraph 0 (1 #178# 1) 12 2 0COMMENT 191) " N1: 2960 " 1)))))COMMENT "PaeR7I: 1 site") 1 5 "CTCGAG" "GAGCTC")COMMENT #431=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #432=(CGroupParCOMMENT (CParagraph 0 (10 #179# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #433=(CLineParCOMMENT (CParagraph 0 (1 #179# 1) 12 2 0COMMENT 191) " N1: 4698 " 1)))))

COMMENT "PflMI: 1 site") 7 4 "CCANNNNNTGG""GGTNNNNNACC")COMMENT #434=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #435=(CGroupParCOMMENT (CParagraph 0 (10 #180# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #436=(CLineParCOMMENT (CParagraph 0 (1 #180# 1) 12 2 0COMMENT 191) " N1: 3616 " 1)))))COMMENT "PinAI: 1 site") 1 5 "ACCGGT" "TGGCCA")COMMENT #437=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #438=(CGroupParCOMMENT (CParagraph 0 (10 #185# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #439=(CLineParCOMMENT (CParagraph 0 (1 #185# 1) 12 2 0COMMENT 191) " N1: 3011 " 1)))))COMMENT "Ppu10I: 1 site") 1 5 "ATGCAT" "TACGTA")COMMENT #440=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #441=(CGroupParCOMMENT (CParagraph 0 (10 #189# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #442=(CLineParCOMMENT (CParagraph 0 (1 #189# 1) 12 2 0COMMENT 191) " N1: 5214 " 1)))))COMMENT "PvuII: 1 site") 3 3 "CAGCTG" "GTCGAC")COMMENT #443=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #444=(CGroupParCOMMENT (CParagraph 0 (10 #194# 0) 1 2 2 0180)

COMMENT (CObjectListCOMMENT #445=(CLineParCOMMENT (CParagraph 0 (1 #194# 1) 12 2 0COMMENT 191) " N1: 4960 " 1)))))COMMENT "SacII: 1 site") 4 2 "CCGCGG" "GGCGCC")COMMENT #446=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #447=(CGroupParCOMMENT (CParagraph 0 (10 #196# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #448=(CLineParCOMMENT (CParagraph 0 (1 #196# 1) 12 2 0COMMENT 191)COMMENT " N1: 1493 (on complementarystrand)"COMMENT 1))))) "SapI: 1 site") 8 11COMMENT "GCTCTTCNNNNN" "CGAGAAGNNNNN")COMMENT #449=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #450=(CGroupParCOMMENT (CParagraph 0 (10 #209# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #451=(CLineParCOMMENT (CParagraph 0 (1 #209# 1) 12 2 0COMMENT 191) " N1: 262 " 1)))))COMMENT "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA")COMMENT #452=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #453=(CGroupParCOMMENT (CParagraph 0 (10 #211# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #454=(CLineParCOMMENT (CParagraph 0 (1 #211# 1) 12 2 0COMMENT 191) " N1: 4338 " 1)))))COMMENT "SspBI: 1 site") 1 5 "TGTACA" "ACATGT")

COMMENT #455=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #456=(CGroupParCOMMENT (CParagraph 0 (10 #222# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #457=(CLineParCOMMENT (CParagraph 0 (1 #222# 1) 12 2 0COMMENT 191) " N1: 4698 " 1)))))COMMENT "Van91I: 1 site") 7 4 "CCANNNNNTGG""GGTNNNNNACC")COMMENT #458=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #459=(CGroupParCOMMENT (CParagraph 0 (10 #223# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #460=(CLineParCOMMENT (CParagraph 0 (1 #223# 1) 12 2 0COMMENT 191) " N1: 2626 " 1)))))COMMENT "XbaI: 1 site") 1 5 "TCTAGA" "AGATCT")COMMENT #461=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178)COMMENT (CObjectListCOMMENT #462=(CGroupParCOMMENT (CParagraph 0 (10 #225# 0) 1 2 2 0180)COMMENT (CObjectListCOMMENT #463=(CLineParCOMMENT (CParagraph 0 (1 #225# 1) 12 2 0COMMENT 191) " N1: 2960 " 1)))))COMMENT "XhoI: 1 site") 1 5 "CTCGAG" "GAGCTC")COMMENT #464=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "ClaI: 2 sites") 2 4"ATCGAT"COMMENT "TAGCTA")COMMENT #465=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Asp700: 2 sites") 5 5COMMENT "GAANNNNTTC" "CTTNNNNAAG")COMMENT #466=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BbsI: 2 sites") 8 12COMMENT "GAAGACNNNNNNN" "CTTCTGNNNNNNN")COMMENT #467=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BcgI: 2 sites") 24 22COMMENT "CGANNNNNNTGCNNNNNNNNNNNNN"COMMENT "GCTNNNNNNACGNNNNNNNNNNNNN")COMMENT #468=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BpuAI: 2 sites") 8 12COMMENT "GAAGACNNNNNNN" "CTTCTGNNNNNNN")COMMENT #469=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsaAI: 2 sites") 3 3"YACGTR"COMMENT "RTGCAY")COMMENT #470=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BspDI: 2 sites") 2 4"ATCGAT"COMMENT "TAGCTA")COMMENT #471=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BspHI: 2 sites") 1 5"TCATGA"COMMENT "AGTACT")COMMENT #472=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsrBI: 2 sites") 3 3"GAGCGG"COMMENT "CTCGCC")COMMENT #473=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "DraII: 2 sites") 2 5"RGGNCCY"

COMMENT "YCCNGGR")COMMENT #474=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "DraIII: 2 sites") 6 3"CACNNNGTG"COMMENT "GTGNNNCAC")COMMENT #475=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EcoO109I: 2 sites") 2 5"RGGNCCY"COMMENT "YCCNGGR")COMMENT #476=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "MfeI: 2 sites") 1 5"CAATTG"COMMENT "GTTAAC")COMMENT #477=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "MunI: 2 sites") 1 5"CAATTG"COMMENT "GTTAAC")COMMENT #478=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NdeI: 2 sites") 2 4"CATATG"COMMENT "GTATAC")COMMENT #479=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NruI: 2 sites") 3 3"TCGCGA"COMMENT "AGCGCT")COMMENT #480=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PpuMI: 2 sites") 2 5"RGGWCCY"COMMENT "YCCWGGR")COMMENT #481=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Psp1406I: 2 sites") 2 4"AACGTT"

COMMENT "TTGCAA")COMMENT #482=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PvuI: 2 sites") 4 2"CGATCG"COMMENT "GCTAGC")COMMENT #483=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "RcaI: 2 sites") 1 5"TCATGA"COMMENT "AGTACT")COMMENT #484=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SalI: 2 sites") 1 5"GTCGAC"COMMENT "CAGCTG")COMMENT #485=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "ScaI: 2 sites") 3 3"AGTACT"COMMENT "TCATGA")COMMENT #486=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SnaBI: 2 sites") 3 3"TACGTA"COMMENT "ATGCAT")COMMENT #487=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SspI: 2 sites") 3 3"AATATT"COMMENT "TTATAA")COMMENT #488=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "XmnI: 2 sites") 5 5"GAANNNNTTC"COMMENT "CTTNNNNAAG")COMMENT #489=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AvaI: 3 sites") 1 5"CYCGRG"

COMMENT "GRGCYC")COMMENT #490=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NcoI: 3 sites") 1 5"CCATGG"COMMENT "GGTACC")COMMENT #491=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AflII: 3 sites") 1 5"CTTAAG"COMMENT "GAATTC")COMMENT #492=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AflIII: 3 sites") 1 5"ACRYGT"COMMENT "TGYRCA")COMMENT #493=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AseI: 3 sites") 2 4"ATTAAT"COMMENT "TAATTA")COMMENT #494=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AsnI: 3 sites") 2 4"ATTAAT"COMMENT "TAATTA")COMMENT #495=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BfrI: 3 sites") 1 5"CTTAAG"COMMENT "GAATTC")COMMENT #496=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsrDI: 3 sites") 8 6"GCAATGNNN"COMMENT "CGTTACNNN")COMMENT #497=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EagI: 3 sites") 1 5"CGGCCG"

COMMENT "GCCGGC")COMMENT #498=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Ecl136II: 3 sites") 3 3"GAGCTC"COMMENT "CTCGAG")COMMENT #499=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EclXI: 3 sites") 1 5"CGGCCG"COMMENT "GCCGGC")COMMENT #500=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EcoRV: 3 sites") 3 3"GATATC"COMMENT "CTATAG")COMMENT #501=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "HaeII: 3 sites") 5 1"RGCGCY"COMMENT "YCGCGR")COMMENT #502=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NaeI: 3 sites") 3 3"GCCGGC"COMMENT "CGGCCG")COMMENT #503=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NgoMI: 3 sites") 1 5"GCCGGC"COMMENT "CGGCCG")COMMENT #504=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NspI: 3 sites") 5 1"RCATGY"COMMENT "YGTACR")COMMENT #505=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SacI: 3 sites") 5 1"GAGCTC"

COMMENT "CTCGAG")COMMENT #506=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "XmaIII: 3 sites") 1 5"CGGCCG"COMMENT "GCCGGC")COMMENT #507=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "ApaLI: 4 sites") 1 5"GTGCAC"COMMENT "CACGTG")COMMENT #508=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AccI: 4 sites") 2 4"GTMKAC"COMMENT "CAKMTG")COMMENT #509=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Alw44I: 4 sites") 1 5"GTGCAC"COMMENT "CACGTG")COMMENT #510=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AlwNI: 4 sites") 6 3"CAGNNNCTG"COMMENT "GTCNNNGAC")COMMENT #511=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BglII: 4 sites") 1 5"AGATCT"COMMENT "TCTAGA")COMMENT #512=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsaI: 4 sites") 7 11COMMENT "GGTCTCNNNNNN" "CCAGAGNNNNNN")COMMENT #513=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EarI: 4 sites") 7 10COMMENT "CTCTTCNNNNN" "GAGAAGNNNNN")COMMENT #514=(CRSFolderPar

COMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "HincII: 4 sites") 3 3"GTYRAC"COMMENT "CARYTG")COMMENT #515=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "HindII: 4 sites") 3 3"GTYRAC"COMMENT "CARYTG")COMMENT #516=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Ksp632I: 4 sites") 7 10COMMENT "CTCTTCNNNNN" "GAGAAGNNNNN")COMMENT #517=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SnoI: 4 sites") 1 5"GTGCAC"COMMENT "CACGTG")COMMENT #518=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "HindIII: 5 sites") 1 5"AAGCTT"COMMENT "TTCGAA")COMMENT #519=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BanII: 5 sites") 5 1"GRGCYC"COMMENT "CYCGRG")COMMENT #520=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BpmI: 5 sites") 22 20COMMENT "CTGGAGNNNNNNNNNNNNNNNNN""GACCTCNNNNNNNNNNNNNNNNN")COMMENT #521=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "DraI: 5 sites") 3 3"TTTAAA"COMMENT "AAATTT")COMMENT #522=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AvaII: 6 sites") 1 4"GGWCC"COMMENT "CCWGG")COMMENT #523=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BanI: 6 sites") 1 5"GGYRCC"COMMENT "CCRYGG")COMMENT #524=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsaWI: 6 sites") 1 5"WCCGGW"COMMENT "WGGCCW")COMMENT #525=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsgI: 6 sites") 22 20COMMENT "GTGCAGNNNNNNNNNNNNNNNNN""CACGTCNNNNNNNNNNNNNNNNN")COMMENT #526=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsrFI: 6 sites") 1 5"RCCGGY"COMMENT "YGGCCR")COMMENT #527=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Cfr10I: 6 sites") 1 5"RCCGGY"COMMENT "YGGCCR")COMMENT #528=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "DsaI: 6 sites") 1 5"CCRYGG"COMMENT "GGYRCC")COMMENT #529=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "EaeI: 6 sites") 1 5"YGGCCR"COMMENT "RCCGGY")COMMENT #530=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "TfiI: 6 sites") 1 4"GAWTC"COMMENT "CTWAG")COMMENT #531=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 7 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AcsI: 7 sites") 1 5"RAATTY"COMMENT "YTTAAR")COMMENT #532=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 7 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "ApoI: 7 sites") 1 5"RAATTY"COMMENT "YTTAAR")COMMENT #533=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 7 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BglI: 7 sites") 7 4"GCCNNNNNGGC"COMMENT "CGGNNNNNCCG")COMMENT #534=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 7 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "MspA1I: 7 sites") 3 3"CMGCKG"COMMENT "GKCGMC")COMMENT #535=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 8 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "Eco57I: 8 sites") 22 20COMMENT "CTGAAGNNNNNNNNNNNNNNNNN""GACTTCNNNNNNNNNNNNNNNNN")COMMENT #536=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 8 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "PleI: 8 sites") 9 10COMMENT "GAGTCNNNNNN" "CTCAGNNNNNN")COMMENT #537=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 8 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "SfcI: 8 sites") 1 5"CTRYAG"COMMENT "GAYRTC")COMMENT #538=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)

COMMENT (CObjectList)) "AatII: 9 sites") 5 1"GACGTC"COMMENT "CTGCAG")COMMENT #539=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "AspHI: 9 sites") 5 1"GWGCWC"COMMENT "CWCGWG")COMMENT #540=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsiEI: 9 sites") 4 2"CGRYCG"COMMENT "GCYRGC")COMMENT #541=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "BsiHKAI: 9 sites") 5 1"GWGCWC"COMMENT "CWCGWG")COMMENT #542=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "HgiAI: 9 sites") 5 1"GWGCWC"COMMENT "CWCGWG")COMMENT #543=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "McrI: 9 sites") 4 2"CGRYCG"COMMENT "GCYRGC")COMMENT #544=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "NciI: 9 sites") 2 3"CCSGG"COMMENT "GGSCC")COMMENT #545=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 9 (8 0) 1 1 1 0 178)COMMENT (CObjectList)) "StyI: 9 sites") 1 5"CCWWGG"COMMENT "GGWWCC")COMMENT #546=(CRSFolderParCOMMENT (CFolderPar

COMMENT (CGroupPar (CParagraph 11 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BsmAI: 11 sites") 6 10COMMENT "GTCTCNNNNNN" "CAGAGNNNNNN")COMMENT #547=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 11 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MslI: 11 sites") 5 5"CAYNNNNRTG"COMMENT "GTRNNNNYAC")COMMENT #548=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 12 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "AcyI: 12 sites") 2 4"GRCGYC"COMMENT "CYGCRG")COMMENT #549=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 12 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BsaHI: 12 sites") 2 4"GRCGYC"COMMENT "CYGCRG")COMMENT #550=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 12 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "FokI: 12 sites") 14 18COMMENT "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN")COMMENT #551=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 13 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "HgaI: 13 sites") 10 15COMMENT "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN")COMMENT #552=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 14 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "HinfI: 14 sites") 1 4"GANTC"COMMENT "CTNAG")COMMENT #553=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 16 (8 0) 1 1 1 0178)

COMMENT (CObjectList)) "HphI: 16 sites") 13 12COMMENT "GGTGANNNNNNNNN" "CCACTNNNNNNNNN")COMMENT #554=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 16 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "SfaNI: 16 sites") 10 14COMMENT "GCATCNNNNNNNNNN" "CGTAGNNNNNNNNNN")COMMENT #555=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 17 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BmyI: 17 sites") 5 1"GDGCHC"COMMENT "CHCGDG")COMMENT #556=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 17 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BsmFI: 17 sites") 15 19COMMENT "GGGACNNNNNNNNNNNNNNN""CCCTGNNNNNNNNNNNNNNN")COMMENT #557=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 17 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "Bsp1286I: 17 sites") 5 1"GDGCHC"COMMENT "CHCGDG")COMMENT #558=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 18 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "DdeI: 18 sites") 1 4"CTNAG"COMMENT "GANTC")COMMENT #559=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 18 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "TaqI: 18 sites") 1 3"TCGA"COMMENT "AGCT")COMMENT #560=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 20 (8 0) 1 1 1 0178)

COMMENT (CObjectList)) "BstYI: 20 sites") 1 5"RGATCY"COMMENT "YCTAGR")COMMENT #561=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 20 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "XhoII: 20 sites") 1 5"RGATCY"COMMENT "YCTAGR")COMMENT #562=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 21 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MaeII: 21 sites") 1 3"ACGT"COMMENT "TGCA")COMMENT #563=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 22 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BsrI: 22 sites") 6 4"ACTGGNN"COMMENT "TGACCNN")COMMENT #564=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 22 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MboII: 22 sites") 13 12COMMENT "GAAGANNNNNNNNN" "CTTCTNNNNNNNNN")COMMENT #565=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BstUI: 23 sites") 2 2"CGCG"COMMENT "GCGC")COMMENT #566=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "FnuDII: 23 sites") 2 2"CGCG"COMMENT "GCGC")COMMENT #567=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)

COMMENT (CObjectList)) "HpaII: 23 sites") 1 3"CCGG"COMMENT "GGCC")COMMENT #568=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MspI: 23 sites") 1 3"CCGG"COMMENT "GGCC")COMMENT #569=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MvnI: 23 sites") 2 2"CGCG"COMMENT "GCGC")COMMENT #570=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "NlaIV: 23 sites") 3 3"GGNNCC"COMMENT "CCNNGG")COMMENT #571=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 23 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "ThaI: 23 sites") 2 2"CGCG"COMMENT "GCGC")COMMENT #572=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 24 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BsiYI: 24 sites") 7 4COMMENT "CCNNNNNNNGG" "GGNNNNNNNCC")COMMENT #573=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 24 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BslI: 24 sites") 7 4COMMENT "CCNNNNNNNGG" "GGNNNNNNNCC")COMMENT #574=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 25 (8 0) 1 1 1 0178)

COMMENT (CObjectList)) "BfaI: 25 sites") 1 3"CTAG"COMMENT "GATC")COMMENT #575=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 25 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MaeI: 25 sites") 1 3"CTAG"COMMENT "GATC")COMMENT #576=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 25 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "MaeIII: 25 sites") 1 6"NGTNACN"COMMENT "NCANTGN")COMMENT #577=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 25 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "Sau96I: 25 sites") 1 4"GGNCC"COMMENT "CCNGG")COMMENT #578=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 26 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "AlwI: 26 sites") 9 10COMMENT "GGATCNNNNNN" "CCTAGNNNNNN")COMMENT #579=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 26 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BsaJI: 26 sites") 1 5"CCNNGG"COMMENT "GGNNCC")COMMENT #580=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 26 (8 0) 1 1 1 0178)COMMENT (CObjectList)) "BstNI: 26 sites") 2 3"CCWGG"COMMENT "GGWCC")COMMENT #581=(CRSFolderParCOMMENT (CFolderParCOMMENT (CGroupPar (CParagraph 26 (8 0) 1 1 1 0178)

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/vntifkey="29" /label=U6\Promotor misc_feature 2826..2859 /vntifkey="21" /label=5'\LoxP misc_feature 4367..4400 /vntifkey="21" /label=3'\LoxP misc_feature 4456..4835 /vntifkey="21" /label=WRE primer 2481..2500 /vntifkey="27" /label=Lox\replace\for primer 2708..2733 /vntifkey="27" /label=Loop\out\for primer 4538..4561 /vntifkey="27" /label=Loop\out\rev primer 493..512 /vntifkey="27" /label=Lox\replace\rev primer 3246..3265 /vntifkey="27" /label=Lox\replace\revBASE COUNT 1999 a 1847 c 1901 g 1820 tORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta

1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2641 cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact 2701 cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg 2761 ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga taggcttgga 2821 tttctataac ttcgtatagc atacattata cgaagttata catgtcacaa aaggaaactc 2881 accctaactg taaagtaatt gtgtgttttg agactataaa tatcccttgg agaaaagcct 2941 tgttaacgcg cggtgaccct cgagtactag gatccattag gcggccgcgt ggataaccgt 3001 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc 3061 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 3121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 3181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 3241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 3301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 3361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 3421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 3481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 3541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca 3601 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 3661 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 3721 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 3781 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc 3841 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 3901 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 3961 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 4021 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc

4081 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 4141 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 4201 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac 4261 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 4321 ctcggcatgg acgagctgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag 4381 catacattat acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat 4441 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt 4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 4861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 5041 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag 5101 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 5161 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 5221 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 5281 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa 5341 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt 5401 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca 5461 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga 5521 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct 5581 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 5641 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5701 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg 5761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 6421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 6481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 6541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 6601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 6721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 6841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 6901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg

6961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 7201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 7261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 7321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 7381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 7501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 7561 acctgac//

LOCUS pSicoR-GFP 7567 bp DNA circular...

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Transcript of LOCUS pSicoR-GFP 7567 bp DNA circular...