Liver & Gall BladderLaboratoryTemple University School of MedicineIntroductionThe purpose of this laboratory is to be able to identify:The histological organization of the liver and the elements needed for the production of bile.The histological organization of the liver for the filtration of blood.The histological organization of the gall bladder.The histological organization of the pancreasLiverIt is difficult to appreciate the often described organization of the liver from slides of the human liver.Classical liver lobules are not readily visualized due in part to scant connective tissue in the human liver and because the human liver is not as regularly arranged as some other animals.However during this laboratory you can see the elements that ma!e up a classic liver lobule a portal lobule as well as a hepatic acinus.Slide 133Image 1Liver " low mag.# central vein $cv% lies in the center of a classical liver lobule. Connective tissue containing branches of portal veins hepatic arteries and bile ducts form the portal triad $pt% and lie at the corners ofa he&agonal classical liver lobule $blac! lines%. #triangular portal lobule $green lines% is arranged around a central bile duct within a portal triad with central veins at the corners. # hepatic acinus $red line% is diamond shaped with two portal triads and two central veins at the corners. It is arranged around branches of the artery vein and bile duct running between the portal triads.ptcvptcvSlide 133Image 2'iver " higher mag.Identify:Central vein Hepatic sinusoids Plates of hepatocytes.Portal veinHepatic artery Bile duct.Slide 133Image 3Liver " higher mag.Identify:Portal triad Portal veinHepatic artery Bile duct.Slide 133Image 4Liver " high mag.Identify:Central vein Hepatic sinusoidHepatocytesSlide 133Image 5Liver, high mag.Identify:Hepatic sinusoid HepatocytesEndothelial cellsKupffer cell. Space of DisseDemoLiver in(ectedIdentify:Central veinPortal triad Hepatic sinusoids. Slide 94Image 1Liver" high mag.This is a section through the liver of a rat that had been in(ected with carbon particles.Identify:Kupffer cells containing carbon of the liver that reside in the hepatic sinusoids.Endothelial cell Sinusoid Space of DisseM of !iverLiverIdentify:Sinusoids Endothelial cellsHepatocytesSpace of Disse"#$EM of LiverEM of LiverLiver Identify:HepatocytesTiht !unctions Bile canaliculus.M of !iverLiverIdentify:Hepatocyte Euchro"atic nucleus.#ouh endoplas"ic reticulu" S"ooth endoplas"ic reticulu" $itochondriaLysoso"es$icro%odiesDemoLiver - L.M. and E.M.Identify:Liver cells &lycoen $itochondria S"ooth endoplas"ic reticulu"Slide 13%Image 1Gall bladder, " low mag.Identify:)ucosa)uscular layer*erimuscular connective tissue +erosa.Slide 13%Image 2Gall bladder " higher mag.Identify:)ucosa of the gall bladder Columnar epithelial cellsSlide 135Image 1Pancreas, H & E, Low & very low mags.Identify:Islets of Lanerhans E'cretory pancreasLo( Lo()ery lo( )ery lo(Slide 135Image 2Pancreas, H & E, Low, med & high mags.Identify: Intercalated ducts +erous acini Centroacinar cells$ed $edHih HihHih HihLo( Lo(Slide 135 Slide 135Image 1 Image 1Pancreas, islets of Langerhans richrome stain - low & med. mags.Identify: Islet of Lanerhans Serous secretory acini$ed $ed Lo( Lo(Slide 135Image 2Pancreas, richrome, med & high mags.Identify: Secretory ranules Centroacinar cells Iintercalated intralo%ular ducts.Hih Hih$ed $ed $ed $edSlide 135Image 3Pancreas, richrome, Low, med & high mags.Identify:Intercalated intralo%ular ducts Interlo%ular ducts.Lo( Lo($ed $edHih Hih