Live Imaging and Other Capabilities of the Bioscience Imaging Facility (BIF)
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Live Imaging and Other Capabilitiesof the Bioscience Imaging Facility (BIF)
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The BIF provides the instruments and expertise needed to visualize molecules in preparations ranging from single cells to entire animals.
• Project specific instrument training & advice
• Consultation on sample preparation, image processing, and data analysis
• Charge-back rates; Collaborations are encouraged
The Bioscience Imaging Facility
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• High NA oil objectives 10x, 20x, 40x, 60x• Seven visible laser lines 446, 458, 488, 514, 561, 640 nm• Four channels or 32-bin spectral detection• Resonant scanner*• Piezo stage*• Live imaging chamber*
*Ideal for live imaging monolayer cell cultures
Rotate and Adj
• High NA oil objectives 10x, 20x, 40x, 60x• Seven visible laser lines 446, 458, 488, 514, 561, 640 nm• Four channel detection
• High NA oil objectives 10x, 20x, 40x, 60x
• Seven visible laser lines 446, 458, 488, 514, 561, 640 nm
• Four channels or 32-bin spectral detection• Resonant scanner & Piezo stage*• Live imaging chamber*
*Ideal for live imaging monolayer cell cultures
• High NA oil objectives 10x, 20x, 40x, 60x
• Seven visible laser lines 405, 458, 488, 514, 561, 640 nm
• Four channel detection
Nikon A1R
Nikon C1+
Confocal Microscopy
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Resonant Galvanometer Piezoelectric z-stage
High Speed Image Acquisition
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Spectral Acquisition
--Document spectra--Remove autofluorescence--Spectral FRET--Create virtual channels
T(λ) = C1•R1(λ) + C2•R2(λ)
Other Uses:
Spectral Unmixing
Fluorophoresin sample
Spectral Detector
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• LWD (500 um) water 40x objective*• Mai Tai DeepSee tunable IR laser*• Four channel non-descanned detection*• Resonant scanner*• Live imaging chamber*• Confocal option Two laser lines (405, 488); four channel detection.
*Ideal for live imaging deep into living tissues
Nikon A1R_MP
Multiphoton Microscopy
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• Phase and PlanFluor objectives* • DS-Ri1 color camera (standard)• Photometrics QuantEM CCD (optional)*• Live imaging chamber*
*Ideal for high sensitivity or high frame rate imaging of monolayer cell cultures
Nikon Ti-S
Wide-field Microscopy
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Microscopic ImagesJuan Martinez, Mark HallBiochemistry
Pablo ZavattieriCivil Engineering
Shawn Liu, Xiaoqi LinBiology
Aaron TaylorBindley
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• Cooled CCD• Range of excitation and emission filters Spans the 450-770 nm
• Isofluorane anesthesia provided
*Ideal for collecting signal from within an organism
IVIS Lumina II
In vivo Optical Imaging
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In vivo Optical Images
Cansu Cimen, Tim RatliffComparative Pathobiology
Dan SzymanskiAgronomy
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• Combined SPECT and x-ray CT Enables SPECT and CT image registration
• Multi-pinhole collimators High resolution imaging (350 um voxels) High sensitivity imaging (kBeq source)
• Data binning relative to biological rhythms Reduces blur
• List mode acquisition and statistical 3-D image reconstruction algorithm. Improves image quality
MiLabs U-SPECT-II/CT
Micro SPECT / CT Imaging
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Principles of microSPECT
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Principles of Statistical Reconstruction
?
Object Space
Projection Space
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Micro SPECT / CT Images
Xin Lu, Shuang LiuHealth Sciences
Charity Wayua, Phil LowChemistry
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Live Imaging Accessories
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For more information:Email: [email protected]: http://www.purdue.edu/discoverypark/bioscience/facilities/imaging/index.php
Thank You!