8/9/2019 LiptoCitro - Phytobiotics and Prebiotics: a new alternative for sustainable aquaculturePhytobiotics and Prebiotics

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May | June 2010

Feature title: LiptoCitro - Phytobiotics and Prebiotics: a new alternative forsustainable aquaculturePhytobiotics and Prebiotics

The International magazine for the aquaculture feed industry

International Aquafeed is published five times a year by Perendale Publishers Ltd of the United Kingdom.All data is published in good faith, based on information received, and while every care is taken to prevent inaccuracies,the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis ofinformation published.Copyright 2009 Perendale Publishers L td. All rights reserved. No par t of this publication may be reproduced in any formor by any means without prior permission of the copyright owner. Printed by Perendale Publishers Ltd. ISSN: 1464-0058

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8/9/2019 LiptoCitro - Phytobiotics and Prebiotics: a new alternative for sustainable aquaculturePhytobiotics and Prebiotics

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Environmentally friendly

LiptoCitro is a prebi-

otic, immunoestimulant and

growth promoter with a

proved antibacterial activity. It can be

used at any stage of the fish produc-

tion cycle and no withdrawal period

is needed because it does not containany antibiotics. It is used at dosage

rates of 2.5Kg/M/tonne and it can be

incorporated in the feed mill during

diet manufacture, or top-dressed on the

feed using oil or molasses at the farm.

It is made of a combination of prebiotics,

plant extracts and plant essences and does

not develop off-flavours in either fish or

crustaceans. LiptoCitro is capable of improv-

ing nutrient digestibility, favouring beneficial

intestinal flora, eliminating pathogenic bacteria,

and promoting the immune system, resulting

in higher resistance to stress and disease and

improved growth, survival, FCR, production

and cost-efficiency, even in the event of bacte-

rial, viral and some parasitic disease outbreaks.

LiptoCitro trial dataTrials have been conducted in laborato-

ries, research centres and farms

with a range of fish and shrimp

species and pathogens.

Trials conducted in the

Veterinary Sanitary Vigilance

Centre (VISAVET) of theComplutense University of

Madrid, Spain showed that

LiptoCitro possessed strong in

vitro antibacterial activity against

a range of pathogenic bacterial

species commonly encountered in

aquaculture production systems

(Table 1).

Trials carried from May to

November in a commer-

cial farm in Spain showed the

efficacy of Liptocitro to con-

trol Haemorrhagic Enteritis

outbreaks in rainbow trout

(Oncorhynchus mykiss). A total

amount of 1,700 MT of commer-

cial feed with LiptoCitro added

at 2.5Kg/MT was

used. All the fish had

the same handling

and usual European

vaccine prophylaxis.

In the control lots,

which were not fed

Liptocitro, several

Enteritis outbreaks

occurred over the

six month trial peri-

od and the fish had

to be treated with

antibiotics. However,

the lots of infected fish that were fed

with LiptoCitro improved significantly,

Table 1: Minimum Inhibitory Concentration (MIC) ofLiptoCitro against bacterial strains tested in VISAVETlaboratory, Spain

Microorganism MIC

Aeromonas hydrophila 3 mg/ml

Aeromonas salmonicida subsp. salmonicida 1 mg/ml

Listonella anguillarum 6 mg/ml

Listonella anguillarum CECT 522 3 mg/ml

Leucothrix mucor DSM 2157T 1 mg/ml

Vibrio penaeicida DSM 14398T 3 mg/ml

Vibrio harveyi CECT 525T 1 mg/ml

Vibrio parahaemolyticus 6 mg/ml

Vibrio ordal i 6 mg/ml

Vibrio vulni ficus 1 mg/ml

Vibrio vulnificus CECT 529 0.1 mg/ml

Vibrio alginolyticus 6 mg/ml

Flavobacterium psycrophilum DSMZ 3660T 3 mg/ml

Edwarsiella ictaluri CECT 885T 13 mg/ml

Brevundimonas diminuta CECT 317T 1 mg/ml

Pseudomonas anguilliseptica 0.1 mg/ml

Pseudomonas fluorescens 3 mg/ml

Yersinia ruckeri 3 mg/ml

Photobacterium damselae subsp. piscicida 3 mg/ml

Photobacterium damselae subsp. damselae 6 mg/ml

Lactococcus garvieae 6 mg/ml

Streptococcus iniae CCUG 27303T 13 mg/ml

Streptococcus difficilis DSZM 16828 13 mg/ml

Streptococcus parauberis 6 mg/ml

Mycobacterium chelonae CCUG 47445T 13 mg/ml

Mycobacterium marinum 0.1 mg/ml

by Santiago de la Cuesta, Ignacio Lpez, Antonio Martnez and Laura Muoz, SpainEmail: liptosa@liptosa.com, Website: www.liptosa.com

7,97%

Phytobiotics and PrebioticsA new alternative for sustainable aquaculture

24 | ItrtIol AquAFeed | May-June 2010 -

F: LiptoCitro

recovered and did not need any antibiotic

treatment at any stage of the trials.

Trials conducted throughout a production

cycle on rainbow trout (Oncorhynchus mykiss),

under commercial conditions and using con-

trol lots, showed 14 percent improvement in

FCR in the fish that were fed with 2.5 Kg/MT

of LiptoCitro. This digestibility improvement

was evaluated in further trials using the same

inclusion rates of Liptocitro in the feed. The

results showed that the cost of the ration

could be reduced without altering FCR. This

was achieved by decreasing the amount of

expensive animal origin protein in the diet andincreasing the inclusion of cheaper vegetable

protein instead.

Six month trials were conducted in four

replicate tanks in the laboratories of the

Agricultural Development and Food Research

Institute IMIDA, in Murcia, Spain. These

trials were carried on European sea bass

(Dicentrarchus labrax) under ideal conditions

and without a disease challenge. The fish

fed three g/kg of Liptocitro showed three

percent improvement in growth and survival

rates and eight percent improvement in FCR

over the fish in the control tanks (statisti-

cally significant at 95 percent confidence level)

(Table 2). Improvements in the haemocrit and

haemoglobin levels of fish blood following a

stress test were also observed.

These trials have shown how a better

intestinal integrity, even under ideal conditions

of low density and very low mortality, results

in better nutrient assimilation thus improving

the feed conversion rate. Therefore, in the

field, a better nutrient

assimilation as a result

of a good intestinal

integrity will have

even more advanta-

geous effects than

those observed under

ideal conditions with

regards the improve-

ment of the growth

and survival rates.

LiptoCitro anti-

bacterial efficiency,

survival and FCR

improvement and

growth and immune

system promoter

effect have also

been tested in vari-

ous shrimp species

and under different

farming and envi-

ronmental condi-tions. These results

can be provided

upon request.

Trials carried

out with LiptoCitro

have shown that the

use of prebiotics

and phytobiotics in

fish and shrimp feed

leads into better

production param-

eters and enables

sustainable aquacul-

ture production on

a long term basis

by reducing the use

of antibiotics and

decreasing fish meal

content in diets.

R e f e r e n c e s

are available upon

request.

Table 2: Effect of prebiotic LiptoCitro on Daily Intake Rate (DIR), FeedConversion Ratio (FCR) and Survival Rate in sea bass (Dicentrarchus labrax).Data from six month laboratory trial using four replicates (eight tanks total)

with 100 fish per tank. *Dosages of 3g/Kg. Values shown are the mean standard error

FeedWithoutLiptoCitro(Control)

Feed WithLiptoCitro*

% Effect(LiptoCitro

vs.Control)

Significance

DIR 1.28 0.04 1.19 0.04 7.03% p

8/9/2019 LiptoCitro - Phytobiotics and Prebiotics: a new alternative for sustainable aquaculturePhytobiotics and Prebiotics

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Selenium is an essential nutrient

for all aquatic diets. The f orm used

directly impacts performance in and

out of the water.

Sel-Plex from Alltech is the only

FDA reviewed and EU approved

organic selenium source on the

market. Sel-Plex supports better

health, performance and product

quality of aquatic species.

Sel-Plex is an esSential part of

aqua diets worldwide.

Carc ass Qualit y, Grow t h, Safet y, H

Traceabil i t y, Genet ic Potent ial , Rel

Health, Prof i tabi l i ty, Ox idat ive Stre

Shelf Life, Nut rit ional Value of Foo

www.alltech.com

For more information, visit www.Sel-Plex.com

or e-mail aquasolutions@alltech.com.

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VOLUME 13 IS S UE 3 2010

Alternative Lipid Sources

in Aquafeeds

Phytobiotics andPrebiotics:

- a newalternative forsustainable

aquaculture

Innovative approachesto reduce feed cost in

aquaculture:- Optimizing nutrientutilization and guthealth

IAF10-03. indd 1 30/04/ 010 16: 17

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